eat-3 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	eat-3
Entrez Gene ID	3880386
Full Name	encodes anonymous transcript-3
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_959144.3	XP_964237.3	AMI1 protein

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_02961	XP_003720745.1
Neurospora crassa	NCU01953	XP_964237.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following eat-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the eat-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12740	XM_959144.3 Latest version!	Neurospora crassa OR74A AMI1 protein (eat-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

eat-3 ( XM_959144.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964237.3 Neurospora crassa OR74A AMI1 protein (eat-3), mRNA.

Price & Availability↑

CloneID	ONf12740
Clone ID Related Accession (Same CDS sequence)	XM_959144.3
Accession Version	XM_959144.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	AMI1 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959144.2.

ATGGCTTCGA GAAGAATGGC CAACTCTTTG GCACCCGACG ACCAGGTCGC GGTCGTGACC 
GCCAGCGACC TGCACGATCC CTTTCTGAGC CCACCGGGAC AAAACAACGC GCGACATTCC 
ACTTTTGACT CCCAACTCTT CTCGCTCGAC CATGGGGCCT CTGCCGAGCA AGTTAAACGA 
GCTCTCAAGG CTCACCTTCA AGATACGGAG CGAAGAATGG AGGAGGCTGG CAAATTGGGC 
ACCGCCTTGG TACAACAGCA ACGAGAACTT GCCGAGCGAC TACGAGAGGT GGAACAGCTA 
CAATCCGAAT CCGAGATAGA CCCCGATTTG CGCCAGAAAC TGGCTGAGAT TGAAAGGGAG 
TACAACGAGG TTGCCAGAGA AAGCGCGCGT GCTTTCCTGC CCAAACAGCG AGTTCCCAGT 
AATGACCCGG CTCAGGCTTC TCCGTCTCTC TTCTCACTAG AAGAGAGGGG CTCAAGGCAT 
CGCTCTGCCA GCCCGTCCAA ATTCGAGAGT CTCGCCTCGT CATCTCCTTC CAAGGTCAGT 
ATGCCGAATC GGAGATCACG CAATCAGCCC GCGAACCGCA TCCACGATAT CGAATTTGCT 
GCCGAAATTA GCACATCGTT GATTGCTCAG GTCCGGAACC TGCAAGCTCT GCTTGCCGAA 
AAGGAAGGAG AGCTCAAGAA CCTCAAGGTC GAGAGATCTC GGTTAGAACT TGAGGCAGAA 
GGCCTTCAAC AACGCATGCG GGCTTTGGAT GAAAGTGAGA GCCGATATAA GGATGAAAAC 
TGGAATCTTG AGACGCAGAT TCACGAACTT TTGGCTGCAC AACGGGAGGG TGCGGATCGC 
GAGAAAAAAC TTCAACAGAC GCTCAACATC GTCCAAGCCG AAAAGAACTC GACTCAGCGA 
GAGCTTGAGG AGCTCAAGCA TAATCAATCC AAACAAGATG AGAAGCATGC TGCCACCATC 
AAGAGCCTCG AAATTGAGCT GGGCACCAGC CGACGAAACG CTGCTCTAGC AGAGAGTGAG 
CGCTTGGCGC TGCAAAAGAA GGTCGACGAG TTGACTCAAC AAAACCAAGA GCTTGCAAGG 
GCATTCGCCC AAGAGCGTGG ACGAGCACTG GAGCGCGAAT CTCGTCATGG CAGCAGCGAC 
GACGACTACC AGATGGCGGT TGATAGCGGC ACACCAGAGC ATTCACCACC ACCGTCGCCG 
GTCAAATTAA CCCCCAGACA CTCTGGACTC GAGTCTGAAA CCTTGAAGAC CTCTCTCGCC 
CATGCCCATC GCACAATTCA GACCCTCAGG ACCAACATTC ATCGCGAGAA AACCGAGAAG 
CTCGAACTGA AGCGCATGCT TCAGGATGCC CGTGATGAGG TTGAGAAGCT GAGGACCGGA 
CCTGATGGCC CACCTCACGG TCGCAAGAAT CGGAAAATGG AGTCCAGAGA ATTTAAGAAG 
CCGAGATTGG GTCAGCTGGG CCTTAACCGC TCGACCAAGA CCGAGGTATT GCCCGTTGCG 
CCCCCTAGCC CAGAGGATCC GGAGTGGGAA GACCAGAGTG AAGCTCCATC CCCTCGTACT 
GTGGCGACTA CCGGCCCGAA CTTGCGTCCC GTTCGCAGCT TCATCCCTAT TGCGGAATCT 
AGCAGCGACC ACTTCGAAAC AGCGAACGAA ACTAGCGATG CGGCCTTCGA GACTGCAGCA 
GAGCGTGCCA CGGAAACAGA AGATTTCCAG ACCGGGGCCG AGGAATTCTC GGATGATGAC 
TCCGACGCGC AAACAGAGAC TGAAGCTTCC ACTGGTGCTG GCCGCCTCAA GCGCCCTTCA 
ATGATGCCTT TGGATGATCC AATCCACCGG TACTCCTTTG ACAGTACCGC TTCGACGTCA 
GCAGACGATG AATATCCCGC TTCTAATGAC CCTCGGATGA TGACTCCATC AGCCCAGCCA 
GCCCAGAGGT TGCGGAGAGG CATCAGCCGT GCTACTCTTG CTTCCAACAG ACGGTCACGA 
CAGCATAGTG TCGATCCAGG CATGCATAGC AGCCCTGGAA GCTTCTTCAA CAACACATCC 
GAGTCCCCGC AACAAGGTCA AAGCTTGTTT GCCGAGCTCA ATGAGTTTGG TAGCGATGAT 
GAATCATCCG TGGGCGCCGC TACGCCAGGT CGAAGAAGCA TCCGGTCCGT TGCTAGCCAG 
AACCGCCCAG TTACACCGCC CCCCAGAGTT CCACGCCTGC CAAGGGCTGT GATGGTTGAT 
AGCGGAATGG TGACCGACCC AGTTGATTTC AGATCTTTGG TCAACACCCC CGGTACGCCT 
TCATCGACGG TGTCCGCCCA AGAAGGTGAT AGAAGTGCCA CCAATATCAA GCGACCTATT 
TCCATGGAAT CGGTCGTTGG ACCTAACGGG TCACCTGCTT CCACTCGAAT GCTCGGGGAC 
GAGCAGGGAC GGGCTGTTTT CGCACAGCCC GTACCGGCCG TATCCTATAG CGATGCCGGC 
GTGCAACATG ACATTCAAAC CACTGCCGTG CAGTTGCTGA GCATTTCTGT TGTTCAATCC 
GAGGACATTG AGCCAACACC TGTAGTACTG AATGTGACCT CGATCGTTTC GACAAACATC 
GAGCCTTTCT TGGACCCTGT TCCTGAACCG GCACCGCTTA CAATGTCAAC TCTCGTTTCA 
GAGGATGTTG AACCTGTACA GGAGCCAGAG CCTGAACCAC TTCCCCTTGG TCTTTCAGGC 
CTCGTTACCG AGGATATTGT ACCTGTTTCG GAACCCGAAC CTGAGCCAGT ACCCCTCATC 
TTGTCCAAGC TCGTTTGTGA GGACGTCGAA CCGGTCTTGG AATTTGTGCC AGAACCAGCA 
CCGCTCACCT TCTCGCAATG TGTTTCTGAA CACATTGAGC CTGTTCTGGA ACCTGTTCCC 
GAATCAGCGC CTCTGACTGT CGCGTCTTTC ATTTCAGAGC ACATTGAGCC GGTCCTCGAG 
CCAGAGCCAC CCGCACCTGA ACCGATTCCC CTCAGCGTAT CGGGCCTTGT TTCCGAGCAA 
GTCGAGCCTC GCGCTGATCC AGAGCCCGAG GTCCGCCCGC CGCCAACACT CAGCATACCA 
ACCATCCTTG CCCAGGACAT TAAGCCGGAT GCTAATGTGT CAGCAACTGT TGACCTTGCC 
TTTTCGCATG TTCTCTCGGA ACAAGTTGAA CCCGTTGAAA TACCAGTGGA AGCACCTACT 
GTGGTCACAG TGGTGGAGGC ACCGAAGGAA ATCAAAGAGA AGGCTCTGCC TGTCACCCTC 
GGCTTCTCGT CGATTGTATC AGAGGATATG GAACCACGAG TCGAGCCTGC GCCCTTGCCG 
GCGCCGTTGT CACTCTCGCC TATCAAGGCA GAAGTTTTGG AGCCCCGTCA GGAACCAGAA 
GAACCGTCAC CGGCTCTTGC TGTTTCGTCT ATCCGGTCAC ACGACTTCGA GCCCGTTGAG 
CTCCCGCCTG TTCCCTTGCC AGCATTGTCG GTATCTTCAG TCCAGGCAAG CGCCATTGAG 
CCCGTTGAGG AAGCACCCAC TCCTTTGCCG ACGTTGTCGA TCTCCTCGCT CCAAACGAAC 
GATATTGAGC CTATCGAGGA ACCGATCGCT CCCATGCCAA CACTGTCGGT CTCCTCAGTC 
CAAGCGAGTG ACATTGAGCC TGTCGAAGAA CCACCTGCTC CTCTGCCTCC CTTGTCAATC 
TCCTCAGTCC AAGCGAGTGA CATTGAGCCT GTCGAAGAAC CACCTGCTCC TCTGCCTCCC 
TTGTCGGTCT CCTCAGTCCA AGCGAGTGAC ATTGAGCCTG TCGAAGAACT GCCGGCTCCT 
TTGCCGCCAC TGTCTATCTC ATCAGTTCAG GCGAGTGACA TCCAACCTGT CGAGCTGCCA 
GCTACTCCCT TGCCACCGCT CTCCATCTCT TCAATCAAGG CGAGCGATAT CAAGCCAGTT 
GAACGACCCA TCGCCCCTCT ACCGGCGCTC TCACTCTCGT CGATACAGGC GAATGACATT 
GAGCCCGAGG AAAGACCATT GACACCACCC CCTGCTATGT CCGTTTCACA AATCCAGCTA 
TTGGATGTCG AACCAGAGAA TGTTCCCGCT ACTCTGCCGC CGACACTCTC TGTTTCAGAA 
ATGCTGGCGC AAGAGATCGA GCCTCTCGCC CTGGAGCCTG CTCTCAGCCT TCCTCCATCA 
ATGGCTTTTG CTGACTGGCA GTCACTGGCT ATCGAGCCTC CCTTGAATCG CAGTCCGAAG 
AGAAATGCTT TCATTATACC CAATGACTCA CAAACTGACG CACCTGTCGA GGGAGGCCTC 
TCGCGGTCTA CTCCCGAGCA AAAGGCCGAC TCACAGCAGA AGGTACAATT GGCTACTGTG 
GACCAGGAAG TGCAAACAGC ATTGACCTCG GAGGCTATTG ACATGCTTTT GATGTCAGGA 
ACTCATCAGT CCCACACTCG TGAATTGGAG AGGTCAGAGT CGTCTTTGAG TACCGGCAGT 
CTCTCGACTA TCAGGAGGCA CCGGAGTTTC CAGGAGATCC CTGAAAGCAT CGTTTCTTCA 
AAGGGCAAGA TGGCGGATTA CAGTACTGCT GTTAACGAAG GAACAACGAC CGTTAGGAGA 
CCAAATAGCT CTGCTAGTAG CAGGGCTTTC ATAACCGATG TTCCACCCCT ACCTTCTAAC 
CACAAGGAAG CCATTGAAGC TGCCAGAACT GGCTCCTCAA GTGGCAACCA ACCAATGACT 
GGTCTCATGG GGCCGCCATT ATTATCGGCC TCTGCGCTCA GGAATCAAAG TCAGGGATTA 
CGACCGCGAA CTCCCAATGG CGATACACGT CCCATGTCTC CCATGTCTTA CGTGTCTGGC 
CATGGCACGC CCACACCCCG TGCCATGGAC AGAGCTGGTT CCGCACATGG AACCATGGCT 
TCCGGTGTTC ATTCACCGTC TCGCTTGACG CAGCATAGCC GCAAGTCATC GGTCTCCTCT 
TTTGTTTCTG AAATGGACAC GCGGTTCCAG ATGCAACAAA ACGGCGACTT TGATCCACAT 
GGGTTCGGCA CGGGTACCGA CCCTCGCATG ATCCAGGCTA TTACCCAGAC CATGATCGGT 
GAATATCTGT GGAAGTATAC TCGCAAGGCT GGCCGTGGAG AGATGTCCGA CAACCGGCAT 
CGCAGATATT TCTGGGTTCA TCCTTACACA CGAACCTTGT ATTGGAGTGA TCGTGACCCC 
TCAGCTGCGG GTCGCAGCGA ATTGAGGGCG AAGAGCGTTC CTATCGAGGC TGTACGGGTT 
GTGACGGATG ATAATCCTAT GCCTCCTGGT TTGCACCGCA AGAGTCTGGT GATCATCTCG 
CCCGGCAGGT CAGTCAAAAT TACCTGTACA ACGGGGCAGC GGCACGAAAC ATGGTTCAAT 
GCACTCTCTT ATTTGCTCCT TCGGACCAAC GAACACGGCC ACGCAGACGC AGAGGAAATA 
GCCGGGCATA TCACGCGAGA TGACATCAAC GATTTCAACC CTCAATACTC GAGGCAAGCG 
CCAAGTGGCT CCCGTGGCGC ACCTTCGCTG TCGTCCTACA ACTCCAGGTC GCTTCGTGTT 
GACTCGCCGG CCCTTGACAT GTCAATGAAT ATTCCGACCC TTACGCCCAA CAGGCAAAGC 
GCCCAGAATC GCCCATCTGT CGGCACTCTT AGTCGGATCA GTGGCTATTG GAAGGAGAGC 
AAATTTGGCA GCATGAGAGG CACCAGGAGC GTTTCTGGTC GAGACACATC TCACGGCATC 
TACGAACAGA GTGAAGTCGA GGACAGCGCT GAGGATGTGA GACAAATGAT TGAACAGCAA 
GACCGAGAAG CCGACCGCCT TGAGAACGTC AGAGCTTGTT GTGATGGGAA ACATGATGTT 
GGCACGTTGA CTAGTGGTTC TAAGCGCGGC CACAATCACC GTACACACCA TCACCATCAC 
CATACACACA GAGGGTCTTC GAGGGGACCT TCAAGAGGAC CTAGTACTAC ACCATCACGG 
GCTGAAACCC TACGATCTGC GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964237.3
CDS	1075..7041
Translation	MASRRMANSLAPDDQVAVVTASDLHDPFLSPPGQNNARHSTFDSQLFSLDHGASAEQVKRALKAHLQDTERRMEEAGKLGTALVQQQRELAERLREVEQLQSESEIDPDLRQKLAEIEREYNEVARESARAFLPKQRVPSNDPAQASPSLFSLEERGSRHRSASPSKFESLASSSPSKVSMPNRRSRNQPANRIHDIEFAAEISTSLIAQVRNLQALLAEKEGELKNLKVERSRLELEAEGLQQRMRALDESESRYKDENWNLETQIHELLAAQREGADREKKLQQTLNIVQAEKNSTQRELEELKHNQSKQDEKHAATIKSLEIELGTSRRNAALAESERLALQKKVDELTQQNQELARAFAQERGRALERESRHGSSDDDYQMAVDSGTPEHSPPPSPVKLTPRHSGLESETLKTSLAHAHRTIQTLRTNIHREKTEKLELKRMLQDARDEVEKLRTGPDGPPHGRKNRKMESREFKKPRLGQLGLNRSTKTEVLPVAPPSPEDPEWEDQSEAPSPRTVATTGPNLRPVRSFIPIAESSSDHFETANETSDAAFETAAERATETEDFQTGAEEFSDDDSDAQTETEASTGAGRLKRPSMMPLDDPIHRYSFDSTASTSADDEYPASNDPRMMTPSAQPAQRLRRGISRATLASNRRSRQHSVDPGMHSSPGSFFNNTSESPQQGQSLFAELNEFGSDDESSVGAATPGRRSIRSVASQNRPVTPPPRVPRLPRAVMVDSGMVTDPVDFRSLVNTPGTPSSTVSAQEGDRSATNIKRPISMESVVGPNGSPASTRMLGDEQGRAVFAQPVPAVSYSDAGVQHDIQTTAVQLLSISVVQSEDIEPTPVVLNVTSIVSTNIEPFLDPVPEPAPLTMSTLVSEDVEPVQEPEPEPLPLGLSGLVTEDIVPVSEPEPEPVPLILSKLVCEDVEPVLEFVPEPAPLTFSQCVSEHIEPVLEPVPESAPLTVASFISEHIEPVLEPEPPAPEPIPLSVSGLVSEQVEPRADPEPEVRPPPTLSIPTILAQDIKPDANVSATVDLAFSHVLSEQVEPVEIPVEAPTVVTVVEAPKEIKEKALPVTLGFSSIVSEDMEPRVEPAPLPAPLSLSPIKAEVLEPRQEPEEPSPALAVSSIRSHDFEPVELPPVPLPALSVSSVQASAIEPVEEAPTPLPTLSISSLQTNDIEPIEEPIAPMPTLSVSSVQASDIEPVEEPPAPLPPLSISSVQASDIEPVEEPPAPLPPLSVSSVQASDIEPVEELPAPLPPLSISSVQASDIQPVELPATPLPPLSISSIKASDIKPVERPIAPLPALSLSSIQANDIEPEERPLTPPPAMSVSQIQLLDVEPENVPATLPPTLSVSEMLAQEIEPLALEPALSLPPSMAFADWQSLAIEPPLNRSPKRNAFIIPNDSQTDAPVEGGLSRSTPEQKADSQQKVQLATVDQEVQTALTSEAIDMLLMSGTHQSHTRELERSESSLSTGSLSTIRRHRSFQEIPESIVSSKGKMADYSTAVNEGTTTVRRPNSSASSRAFITDVPPLPSNHKEAIEAARTGSSSGNQPMTGLMGPPLLSASALRNQSQGLRPRTPNGDTRPMSPMSYVSGHGTPTPRAMDRAGSAHGTMASGVHSPSRLTQHSRKSSVSSFVSEMDTRFQMQQNGDFDPHGFGTGTDPRMIQAITQTMIGEYLWKYTRKAGRGEMSDNRHRRYFWVHPYTRTLYWSDRDPSAAGRSELRAKSVPIEAVRVVTDDNPMPPGLHRKSLVIISPGRSVKITCTTGQRHETWFNALSYLLLRTNEHGHADAEEIAGHITRDDINDFNPQYSRQAPSGSRGAPSLSSYNSRSLRVDSPALDMSMNIPTLTPNRQSAQNRPSVGTLSRISGYWKESKFGSMRGTRSVSGRDTSHGIYEQSEVEDSAEDVRQMIEQQDREADRLENVRACCDGKHDVGTLTSGSKRGHNHRTHHHHHHTHRGSSRGPSRGPSTTPSRAETLRSAA

Target ORF information:

RefSeq Version	XM_959144.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A AMI1 protein (eat-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_959144.3

ORF Insert Sequence:

ATGGCTTCGA GAAGAATGGC CAACTCTTTG GCACCCGACG ACCAGGTCGC GGTCGTGACC 
GCCAGCGACC TGCACGATCC CTTTCTGAGC CCACCGGGAC AAAACAACGC GCGACATTCC 
ACTTTTGACT CCCAACTCTT CTCGCTCGAC CATGGGGCCT CTGCCGAGCA AGTTAAACGA 
GCTCTCAAGG CTCACCTTCA AGATACGGAG CGAAGAATGG AGGAGGCTGG CAAATTGGGC 
ACCGCCTTGG TACAACAGCA ACGAGAACTT GCCGAGCGAC TACGAGAGGT GGAACAGCTA 
CAATCCGAAT CCGAGATAGA CCCCGATTTG CGCCAGAAAC TGGCTGAGAT TGAAAGGGAG 
TACAACGAGG TTGCCAGAGA AAGCGCGCGT GCTTTCCTGC CCAAACAGCG AGTTCCCAGT 
AATGACCCGG CTCAGGCTTC TCCGTCTCTC TTCTCACTAG AAGAGAGGGG CTCAAGGCAT 
CGCTCTGCCA GCCCGTCCAA ATTCGAGAGT CTCGCCTCGT CATCTCCTTC CAAGGTCAGT 
ATGCCGAATC GGAGATCACG CAATCAGCCC GCGAACCGCA TCCACGATAT CGAATTTGCT 
GCCGAAATTA GCACATCGTT GATTGCTCAG GTCCGGAACC TGCAAGCTCT GCTTGCCGAA 
AAGGAAGGAG AGCTCAAGAA CCTCAAGGTC GAGAGATCTC GGTTAGAACT TGAGGCAGAA 
GGCCTTCAAC AACGCATGCG GGCTTTGGAT GAAAGTGAGA GCCGATATAA GGATGAAAAC 
TGGAATCTTG AGACGCAGAT TCACGAACTT TTGGCTGCAC AACGGGAGGG TGCGGATCGC 
GAGAAAAAAC TTCAACAGAC GCTCAACATC GTCCAAGCCG AAAAGAACTC GACTCAGCGA 
GAGCTTGAGG AGCTCAAGCA TAATCAATCC AAACAAGATG AGAAGCATGC TGCCACCATC 
AAGAGCCTCG AAATTGAGCT GGGCACCAGC CGACGAAACG CTGCTCTAGC AGAGAGTGAG 
CGCTTGGCGC TGCAAAAGAA GGTCGACGAG TTGACTCAAC AAAACCAAGA GCTTGCAAGG 
GCATTCGCCC AAGAGCGTGG ACGAGCACTG GAGCGCGAAT CTCGTCATGG CAGCAGCGAC 
GACGACTACC AGATGGCGGT TGATAGCGGC ACACCAGAGC ATTCACCACC ACCGTCGCCG 
GTCAAATTAA CCCCCAGACA CTCTGGACTC GAGTCTGAAA CCTTGAAGAC CTCTCTCGCC 
CATGCCCATC GCACAATTCA GACCCTCAGG ACCAACATTC ATCGCGAGAA AACCGAGAAG 
CTCGAACTGA AGCGCATGCT TCAGGATGCC CGTGATGAGG TTGAGAAGCT GAGGACCGGA 
CCTGATGGCC CACCTCACGG TCGCAAGAAT CGGAAAATGG AGTCCAGAGA ATTTAAGAAG 
CCGAGATTGG GTCAGCTGGG CCTTAACCGC TCGACCAAGA CCGAGGTATT GCCCGTTGCG 
CCCCCTAGCC CAGAGGATCC GGAGTGGGAA GACCAGAGTG AAGCTCCATC CCCTCGTACT 
GTGGCGACTA CCGGCCCGAA CTTGCGTCCC GTTCGCAGCT TCATCCCTAT TGCGGAATCT 
AGCAGCGACC ACTTCGAAAC AGCGAACGAA ACTAGCGATG CGGCCTTCGA GACTGCAGCA 
GAGCGTGCCA CGGAAACAGA AGATTTCCAG ACCGGGGCCG AGGAATTCTC GGATGATGAC 
TCCGACGCGC AAACAGAGAC TGAAGCTTCC ACTGGTGCTG GCCGCCTCAA GCGCCCTTCA 
ATGATGCCTT TGGATGATCC AATCCACCGG TACTCCTTTG ACAGTACCGC TTCGACGTCA 
GCAGACGATG AATATCCCGC TTCTAATGAC CCTCGGATGA TGACTCCATC AGCCCAGCCA 
GCCCAGAGGT TGCGGAGAGG CATCAGCCGT GCTACTCTTG CTTCCAACAG ACGGTCACGA 
CAGCATAGTG TCGATCCAGG CATGCATAGC AGCCCTGGAA GCTTCTTCAA CAACACATCC 
GAGTCCCCGC AACAAGGTCA AAGCTTGTTT GCCGAGCTCA ATGAGTTTGG TAGCGATGAT 
GAATCATCCG TGGGCGCCGC TACGCCAGGT CGAAGAAGCA TCCGGTCCGT TGCTAGCCAG 
AACCGCCCAG TTACACCGCC CCCCAGAGTT CCACGCCTGC CAAGGGCTGT GATGGTTGAT 
AGCGGAATGG TGACCGACCC AGTTGATTTC AGATCTTTGG TCAACACCCC CGGTACGCCT 
TCATCGACGG TGTCCGCCCA AGAAGGTGAT AGAAGTGCCA CCAATATCAA GCGACCTATT 
TCCATGGAAT CGGTCGTTGG ACCTAACGGG TCACCTGCTT CCACTCGAAT GCTCGGGGAC 
GAGCAGGGAC GGGCTGTTTT CGCACAGCCC GTACCGGCCG TATCCTATAG CGATGCCGGC 
GTGCAACATG ACATTCAAAC CACTGCCGTG CAGTTGCTGA GCATTTCTGT TGTTCAATCC 
GAGGACATTG AGCCAACACC TGTAGTACTG AATGTGACCT CGATCGTTTC GACAAACATC 
GAGCCTTTCT TGGACCCTGT TCCTGAACCG GCACCGCTTA CAATGTCAAC TCTCGTTTCA 
GAGGATGTTG AACCTGTACA GGAGCCAGAG CCTGAACCAC TTCCCCTTGG TCTTTCAGGC 
CTCGTTACCG AGGATATTGT ACCTGTTTCG GAACCCGAAC CTGAGCCAGT ACCCCTCATC 
TTGTCCAAGC TCGTTTGTGA GGACGTCGAA CCGGTCTTGG AATTTGTGCC AGAACCAGCA 
CCGCTCACCT TCTCGCAATG TGTTTCTGAA CACATTGAGC CTGTTCTGGA ACCTGTTCCC 
GAATCAGCGC CTCTGACTGT CGCGTCTTTC ATTTCAGAGC ACATTGAGCC GGTCCTCGAG 
CCAGAGCCAC CCGCACCTGA ACCGATTCCC CTCAGCGTAT CGGGCCTTGT TTCCGAGCAA 
GTCGAGCCTC GCGCTGATCC AGAGCCCGAG GTCCGCCCGC CGCCAACACT CAGCATACCA 
ACCATCCTTG CCCAGGACAT TAAGCCGGAT GCTAATGTGT CAGCAACTGT TGACCTTGCC 
TTTTCGCATG TTCTCTCGGA ACAAGTTGAA CCCGTTGAAA TACCAGTGGA AGCACCTACT 
GTGGTCACAG TGGTGGAGGC ACCGAAGGAA ATCAAAGAGA AGGCTCTGCC TGTCACCCTC 
GGCTTCTCGT CGATTGTATC AGAGGATATG GAACCACGAG TCGAGCCTGC GCCCTTGCCG 
GCGCCGTTGT CACTCTCGCC TATCAAGGCA GAAGTTTTGG AGCCCCGTCA GGAACCAGAA 
GAACCGTCAC CGGCTCTTGC TGTTTCGTCT ATCCGGTCAC ACGACTTCGA GCCCGTTGAG 
CTCCCGCCTG TTCCCTTGCC AGCATTGTCG GTATCTTCAG TCCAGGCAAG CGCCATTGAG 
CCCGTTGAGG AAGCACCCAC TCCTTTGCCG ACGTTGTCGA TCTCCTCGCT CCAAACGAAC 
GATATTGAGC CTATCGAGGA ACCGATCGCT CCCATGCCAA CACTGTCGGT CTCCTCAGTC 
CAAGCGAGTG ACATTGAGCC TGTCGAAGAA CCACCTGCTC CTCTGCCTCC CTTGTCAATC 
TCCTCAGTCC AAGCGAGTGA CATTGAGCCT GTCGAAGAAC CACCTGCTCC TCTGCCTCCC 
TTGTCGGTCT CCTCAGTCCA AGCGAGTGAC ATTGAGCCTG TCGAAGAACT GCCGGCTCCT 
TTGCCGCCAC TGTCTATCTC ATCAGTTCAG GCGAGTGACA TCCAACCTGT CGAGCTGCCA 
GCTACTCCCT TGCCACCGCT CTCCATCTCT TCAATCAAGG CGAGCGATAT CAAGCCAGTT 
GAACGACCCA TCGCCCCTCT ACCGGCGCTC TCACTCTCGT CGATACAGGC GAATGACATT 
GAGCCCGAGG AAAGACCATT GACACCACCC CCTGCTATGT CCGTTTCACA AATCCAGCTA 
TTGGATGTCG AACCAGAGAA TGTTCCCGCT ACTCTGCCGC CGACACTCTC TGTTTCAGAA 
ATGCTGGCGC AAGAGATCGA GCCTCTCGCC CTGGAGCCTG CTCTCAGCCT TCCTCCATCA 
ATGGCTTTTG CTGACTGGCA GTCACTGGCT ATCGAGCCTC CCTTGAATCG CAGTCCGAAG 
AGAAATGCTT TCATTATACC CAATGACTCA CAAACTGACG CACCTGTCGA GGGAGGCCTC 
TCGCGGTCTA CTCCCGAGCA AAAGGCCGAC TCACAGCAGA AGGTACAATT GGCTACTGTG 
GACCAGGAAG TGCAAACAGC ATTGACCTCG GAGGCTATTG ACATGCTTTT GATGTCAGGA 
ACTCATCAGT CCCACACTCG TGAATTGGAG AGGTCAGAGT CGTCTTTGAG TACCGGCAGT 
CTCTCGACTA TCAGGAGGCA CCGGAGTTTC CAGGAGATCC CTGAAAGCAT CGTTTCTTCA 
AAGGGCAAGA TGGCGGATTA CAGTACTGCT GTTAACGAAG GAACAACGAC CGTTAGGAGA 
CCAAATAGCT CTGCTAGTAG CAGGGCTTTC ATAACCGATG TTCCACCCCT ACCTTCTAAC 
CACAAGGAAG CCATTGAAGC TGCCAGAACT GGCTCCTCAA GTGGCAACCA ACCAATGACT 
GGTCTCATGG GGCCGCCATT ATTATCGGCC TCTGCGCTCA GGAATCAAAG TCAGGGATTA 
CGACCGCGAA CTCCCAATGG CGATACACGT CCCATGTCTC CCATGTCTTA CGTGTCTGGC 
CATGGCACGC CCACACCCCG TGCCATGGAC AGAGCTGGTT CCGCACATGG AACCATGGCT 
TCCGGTGTTC ATTCACCGTC TCGCTTGACG CAGCATAGCC GCAAGTCATC GGTCTCCTCT 
TTTGTTTCTG AAATGGACAC GCGGTTCCAG ATGCAACAAA ACGGCGACTT TGATCCACAT 
GGGTTCGGCA CGGGTACCGA CCCTCGCATG ATCCAGGCTA TTACCCAGAC CATGATCGGT 
GAATATCTGT GGAAGTATAC TCGCAAGGCT GGCCGTGGAG AGATGTCCGA CAACCGGCAT 
CGCAGATATT TCTGGGTTCA TCCTTACACA CGAACCTTGT ATTGGAGTGA TCGTGACCCC 
TCAGCTGCGG GTCGCAGCGA ATTGAGGGCG AAGAGCGTTC CTATCGAGGC TGTACGGGTT 
GTGACGGATG ATAATCCTAT GCCTCCTGGT TTGCACCGCA AGAGTCTGGT GATCATCTCG 
CCCGGCAGGT CAGTCAAAAT TACCTGTACA ACGGGGCAGC GGCACGAAAC ATGGTTCAAT 
GCACTCTCTT ATTTGCTCCT TCGGACCAAC GAACACGGCC ACGCAGACGC AGAGGAAATA 
GCCGGGCATA TCACGCGAGA TGACATCAAC GATTTCAACC CTCAATACTC GAGGCAAGCG 
CCAAGTGGCT CCCGTGGCGC ACCTTCGCTG TCGTCCTACA ACTCCAGGTC GCTTCGTGTT 
GACTCGCCGG CCCTTGACAT GTCAATGAAT ATTCCGACCC TTACGCCCAA CAGGCAAAGC 
GCCCAGAATC GCCCATCTGT CGGCACTCTT AGTCGGATCA GTGGCTATTG GAAGGAGAGC 
AAATTTGGCA GCATGAGAGG CACCAGGAGC GTTTCTGGTC GAGACACATC TCACGGCATC 
TACGAACAGA GTGAAGTCGA GGACAGCGCT GAGGATGTGA GACAAATGAT TGAACAGCAA 
GACCGAGAAG CCGACCGCCT TGAGAACGTC AGAGCTTGTT GTGATGGGAA ACATGATGTT 
GGCACGTTGA CTAGTGGTTC TAAGCGCGGC CACAATCACC GTACACACCA TCACCATCAC 
CATACACACA GAGGGTCTTC GAGGGGACCT TCAAGAGGAC CTAGTACTAC ACCATCACGG 
GCTGAAACCC TACGATCTGC GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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eat-3 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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