nit-3 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	nit-3
Entrez Gene ID	3878217
Full Name	nitrate nonutilizer-3
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_956976.2	XP_962069.1	nitrate reductase

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06062	XP_003711915.1
Oryza sativa (rice)	Os02g0770800	NP_001048253.1
Neurospora crassa	NCU05298	XP_962069.1
Oryza sativa (rice)	Os08g0468100	NP_001062006.1
Arabidopsis thaliana (thale cress)	NIA2	NP_174901.1
Arabidopsis thaliana (thale cress)	NIA1	NP_177899.1
Oryza sativa (rice)	Os08g0468700	NP_001062009.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

analysis of molybdenum cofactor-free nitrate reductase from Neurospora crassa
Title: Biochemical characterization of molybdenum cofactor-free nitrate reductase from Neurospora crassa.

The following nit-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nit-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf03716	XM_956976.2 Latest version!	Neurospora crassa OR74A nitrate reductase (nit-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nit-3 ( XM_956976.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_962069.1 Neurospora crassa OR74A nitrate reductase (nit-3), mRNA.

Price & Availability↑

CloneID	ONf03716
Clone ID Related Accession (Same CDS sequence)	XM_956976.2
Accession Version	XM_956976.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	nitrate reductase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_956976.1.

ATGGAGGCTC CAGCTCTCGA GCAGCGTCAA AGTCTTCATG ACTCCTCCGA ACGTCAACAG 
AGATTCACAT CTCTCATCTT GCCAAATGGC GCTGCTGCTG CAGCAGCAGA GAAGAACCCC 
AGGGGAGCGG CGGACTACTG TCCGTCCGAC AATGACAATG ACATTGACAA CGACCTCGCC 
AGCACTCGCA CCGCCAGCCC GACTACAACG GACTTCTCTT CCTCCTCATC CGACGACAAC 
TCCACAACAT TAGAGACCTC GGTTAACTAC TCACACTCAT CCAACACCAA CACCAACACC 
TCCTGCCCGC CCTCCCCAAT AACCTCTTCA TCACTCAAAC CAGCCTACCC CCTCCCCCCA 
CCCTCCACCC GACTAACCAC CATCCTCCCC ACCGACCTCA AAACCCCCGA CCACCTCATC 
CCGCGCGACC CGCGCCTCAT TCGCCTCACG GGATCCCACC CCTTCAACGT CGAACCGCCG 
CTCACTGCGC TTTTCGAACA CGGATTCCTA ACCCCGCAAA ACCTCCATTA CGTGCGCAAC 
CACGGGCCCA TCCCTTCATC TGTCGCTACC CCTCCCGCTA CCATCAATAA GGAGGAAGAC 
GACTCGCTAC TAAACTGGGA ATTCACCGTC GAAGGACTCG TCGAGCACCC CCTAAAAATC 
AGCGTGCGGG AGTTGATGGA CGCTTCCAAA TGGGACAACG TCACGTACCC CGTCACTTTA 
GTGTGCGCGG GCAACAGGAG GAAAGAACAA AACGTGCTCC GAAAATCGAA GGGGTTCTCA 
TGGGGCGCGG GCGGGCTATC GACTGCGTTG TGGACGGGGG TGGGACTTTC TGAGATATTG 
GCGAGGGCAA AACCTCTAAC GAAAAAGGGA GGAGGGGCGA GGTACGTCTG CTTTGAGGGC 
GCCGATCAGC TGCCGAATGG GACGTACGGA ACCAGTGTCA AGCTTGCGTG GGCGATGGAT 
CCCAACAAGG GGATCATGGT GGCGCATAAG ATGAATGGAG AGAACTTGCA TCCTGATCAT 
GGACGGCCGG TCAGGGTGGT GGTCCCGGGA CAGATTGGTG GACGGAGTGT CAAGTGGTTG 
AAGAGGATTG TGGTCACCAA GGGGCCGAGC GAGAATTGGT ATCATGTTTT TGATAATAGG 
GTGTTGCCGA CGACGGTGGG GCCGGAGGAG AGTGGGGAGA AGACGGAGGA GATGGAGAGG 
GTGTGGAGGG ATGAGAGGTA TGCGATTTAT GATTTGAATG TGAATAGTGT GATTTGTGAG 
CCGGGGCATG GGGAGGTTGT GTCGCTGAGA GGGGACGAAG GGGCGGGGAC GTACAGGTTG 
AGGGGATATG CGTATGCGGG GGGAGGGAGG AGGGTGACGA GGTTGGAGGT GACGCTTGAT 
CAGGGGAAGA GCTGGAGGCT GGCGGGCATT GAGTACCCAG AGGATCGGTA TCGGGAAGCT 
CAAGATGGGG AGGAGTTGTT TGGTGGAAGG TTGGATGTGT CGTGGCGGGA GAGCTGCTTT 
TGTTGGTGTT TCTGGGATTT GGAGATTCCT CTTTCTGAGC TGCGCAAGGC AAAGGATGTG 
TGCATCCGAG CGATGGATGA GTCGCTGGCG CTACAGCCCA AGGAGATGTA TTGGAGTGTG 
CTGGGGATGA TGAATAACCC TTGGTTTCGG GTCGTTATTC ATCATGAGGG CGATACCTTG 
CGTTTCGAGC ATCCTACTCA ACCGATGCTG ACGTCGGACG GTTGGATGGA TCGAGTGAAG 
AAGGAAGGCG GGAATCTTGC TAATGGCTTT TGGGGCGAGA AAGTCCCCGG GGCTGAGGAG 
AACGTCGTCA AAGAAGAGCC TGTCAAGGAG ATCAGCATGG TGGATGAAAA AGTCACTCGA 
CTTATCACAC TTGAAGAGCT CCGCCAGCAC GATGGTGAGG AAGAGCCCTG GTTCGTCGTG 
AACGGCCAAG TTTACAACGG TACGCCGTTC CTCGAGGGTC ATCCAGGTGG TGCTGCCTCC 
ATCACCGGCG CTGCTGGACA GGACGTAACC GACGAGTTCC TAGCCATTCA CAGCGAAAAT 
GCCAAAGCAA TGATGCCAAC CTACCACATC GGCACCCTCA CTCCCTCCGC CCTCGCGGCG 
CTCAAGTCCT CCTCCACCTC GGACCCAGCT CTCAGCGATC CCAGCCGCCC CATCTTCCTC 
CAGTCCAAGA CATGGAACAG CGCCATCCTC ACCTTCAAAG AATCCGTCTC CCCGGATACC 
AAAATCTTCC ACTTCGCTTT GTCACACCCT GCCCAATCCA TTGGGCTCCC CGTCGGCCAA 
CATCTCATGA TGCGACTCCC TGACCCTGCT AAACCCACCG AATCTATCAT CCGCGCTTAC 
ACGCCCATCT CGGACGGCAC TTTGGAGCGG GGAACTCTCC GGGTGTTGGT CAAGATCTAC 
TACGCTTCTC CTACCGAAGA TATAAAGGGA GGACAAATGA CACAGGCGCT GGATGCTTTG 
GCACTGGGGA AAGCCGTGGA GTTCAAAGGA CCGGTGGGCA AGTTTGTCTA CCAAGGCCGG 
GGCGTCTGCT CTGTCAATGG ACGAGAGAGG AAGGTCAAGA GGTTTGTCAT GGTTTGTGGT 
GGGTCGGGCG TGACGCCTAT CTACCAGGTG CTGAGGGCAG TTGCCGTGGA TGACCAAGAC 
GGGACGGAGT GTTTGGTGTT GGATGGGAAT CGGGTAGAAG GAGACATTCT GATGAAGAGC 
GAGTTGGATG AGTTGGTGGA GAGGGCCAAA CCCGAGGGGA GGTGTAGGGT CAAGTATACT 
TTGAGTAGAC CGGGTGCAGA GTGGGAAGGG CTGAGAGGAA GGCTGGATAA GACGATGCTG 
GAAAGAGAGG TTGGAGAAGG GGATTTGAGG GGGGAGACGA TGGTGTTGCT GTGTGGGCCG 
GAGGGAATGC AGAATATGGT TCGGGAGGTG TTGAAGGGGA TGGGGTGGAA GGATGAGGAT 
GTATTAGTTT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_962069.1
CDS	144..3098
Translation	MEAPALEQRQSLHDSSERQQRFTSLILPNGAAAAAAEKNPRGAADYCPSDNDNDIDNDLASTRTASPTTTDFSSSSSDDNSTTLETSVNYSHSSNTNTNTSCPPSPITSSSLKPAYPLPPPSTRLTTILPTDLKTPDHLIPRDPRLIRLTGSHPFNVEPPLTALFEHGFLTPQNLHYVRNHGPIPSSVATPPATINKEEDDSLLNWEFTVEGLVEHPLKISVRELMDASKWDNVTYPVTLVCAGNRRKEQNVLRKSKGFSWGAGGLSTALWTGVGLSEILARAKPLTKKGGGARYVCFEGADQLPNGTYGTSVKLAWAMDPNKGIMVAHKMNGENLHPDHGRPVRVVVPGQIGGRSVKWLKRIVVTKGPSENWYHVFDNRVLPTTVGPEESGEKTEEMERVWRDERYAIYDLNVNSVICEPGHGEVVSLRGDEGAGTYRLRGYAYAGGGRRVTRLEVTLDQGKSWRLAGIEYPEDRYREAQDGEELFGGRLDVSWRESCFCWCFWDLEIPLSELRKAKDVCIRAMDESLALQPKEMYWSVLGMMNNPWFRVVIHHEGDTLRFEHPTQPMLTSDGWMDRVKKEGGNLANGFWGEKVPGAEENVVKEEPVKEISMVDEKVTRLITLEELRQHDGEEEPWFVVNGQVYNGTPFLEGHPGGAASITGAAGQDVTDEFLAIHSENAKAMMPTYHIGTLTPSALAALKSSSTSDPALSDPSRPIFLQSKTWNSAILTFKESVSPDTKIFHFALSHPAQSIGLPVGQHLMMRLPDPAKPTESIIRAYTPISDGTLERGTLRVLVKIYYASPTEDIKGGQMTQALDALALGKAVEFKGPVGKFVYQGRGVCSVNGRERKVKRFVMVCGGSGVTPIYQVLRAVAVDDQDGTECLVLDGNRVEGDILMKSELDELVERAKPEGRCRVKYTLSRPGAEWEGLRGRLDKTMLEREVGEGDLRGETMVLLCGPEGMQNMVREVLKGMGWKDEDVLVF

Target ORF information:

RefSeq Version	XM_956976.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A nitrate reductase (nit-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_956976.2

ORF Insert Sequence:

ATGGAGGCTC CAGCTCTCGA GCAGCGTCAA AGTCTTCATG ACTCCTCCGA ACGTCAACAG 
AGATTCACAT CTCTCATCTT GCCAAATGGC GCTGCTGCTG CAGCAGCAGA GAAGAACCCC 
AGGGGAGCGG CGGACTACTG TCCGTCCGAC AATGACAATG ACATTGACAA CGACCTCGCC 
AGCACTCGCA CCGCCAGCCC GACTACAACG GACTTCTCTT CCTCCTCATC CGACGACAAC 
TCCACAACAT TAGAGACCTC GGTTAACTAC TCACACTCAT CCAACACCAA CACCAACACC 
TCCTGCCCGC CCTCCCCAAT AACCTCTTCA TCACTCAAAC CAGCCTACCC CCTCCCCCCA 
CCCTCCACCC GACTAACCAC CATCCTCCCC ACCGACCTCA AAACCCCCGA CCACCTCATC 
CCGCGCGACC CGCGCCTCAT TCGCCTCACG GGATCCCACC CCTTCAACGT CGAACCGCCG 
CTCACTGCGC TTTTCGAACA CGGATTCCTA ACCCCGCAAA ACCTCCATTA CGTGCGCAAC 
CACGGGCCCA TCCCTTCATC TGTCGCTACC CCTCCCGCTA CCATCAATAA GGAGGAAGAC 
GACTCGCTAC TAAACTGGGA ATTCACCGTC GAAGGACTCG TCGAGCACCC CCTAAAAATC 
AGCGTGCGGG AGTTGATGGA CGCTTCCAAA TGGGACAACG TCACGTACCC CGTCACTTTA 
GTGTGCGCGG GCAACAGGAG GAAAGAACAA AACGTGCTCC GAAAATCGAA GGGGTTCTCA 
TGGGGCGCGG GCGGGCTATC GACTGCGTTG TGGACGGGGG TGGGACTTTC TGAGATATTG 
GCGAGGGCAA AACCTCTAAC GAAAAAGGGA GGAGGGGCGA GGTACGTCTG CTTTGAGGGC 
GCCGATCAGC TGCCGAATGG GACGTACGGA ACCAGTGTCA AGCTTGCGTG GGCGATGGAT 
CCCAACAAGG GGATCATGGT GGCGCATAAG ATGAATGGAG AGAACTTGCA TCCTGATCAT 
GGACGGCCGG TCAGGGTGGT GGTCCCGGGA CAGATTGGTG GACGGAGTGT CAAGTGGTTG 
AAGAGGATTG TGGTCACCAA GGGGCCGAGC GAGAATTGGT ATCATGTTTT TGATAATAGG 
GTGTTGCCGA CGACGGTGGG GCCGGAGGAG AGTGGGGAGA AGACGGAGGA GATGGAGAGG 
GTGTGGAGGG ATGAGAGGTA TGCGATTTAT GATTTGAATG TGAATAGTGT GATTTGTGAG 
CCGGGGCATG GGGAGGTTGT GTCGCTGAGA GGGGACGAAG GGGCGGGGAC GTACAGGTTG 
AGGGGATATG CGTATGCGGG GGGAGGGAGG AGGGTGACGA GGTTGGAGGT GACGCTTGAT 
CAGGGGAAGA GCTGGAGGCT GGCGGGCATT GAGTACCCAG AGGATCGGTA TCGGGAAGCT 
CAAGATGGGG AGGAGTTGTT TGGTGGAAGG TTGGATGTGT CGTGGCGGGA GAGCTGCTTT 
TGTTGGTGTT TCTGGGATTT GGAGATTCCT CTTTCTGAGC TGCGCAAGGC AAAGGATGTG 
TGCATCCGAG CGATGGATGA GTCGCTGGCG CTACAGCCCA AGGAGATGTA TTGGAGTGTG 
CTGGGGATGA TGAATAACCC TTGGTTTCGG GTCGTTATTC ATCATGAGGG CGATACCTTG 
CGTTTCGAGC ATCCTACTCA ACCGATGCTG ACGTCGGACG GTTGGATGGA TCGAGTGAAG 
AAGGAAGGCG GGAATCTTGC TAATGGCTTT TGGGGCGAGA AAGTCCCCGG GGCTGAGGAG 
AACGTCGTCA AAGAAGAGCC TGTCAAGGAG ATCAGCATGG TGGATGAAAA AGTCACTCGA 
CTTATCACAC TTGAAGAGCT CCGCCAGCAC GATGGTGAGG AAGAGCCCTG GTTCGTCGTG 
AACGGCCAAG TTTACAACGG TACGCCGTTC CTCGAGGGTC ATCCAGGTGG TGCTGCCTCC 
ATCACCGGCG CTGCTGGACA GGACGTAACC GACGAGTTCC TAGCCATTCA CAGCGAAAAT 
GCCAAAGCAA TGATGCCAAC CTACCACATC GGCACCCTCA CTCCCTCCGC CCTCGCGGCG 
CTCAAGTCCT CCTCCACCTC GGACCCAGCT CTCAGCGATC CCAGCCGCCC CATCTTCCTC 
CAGTCCAAGA CATGGAACAG CGCCATCCTC ACCTTCAAAG AATCCGTCTC CCCGGATACC 
AAAATCTTCC ACTTCGCTTT GTCACACCCT GCCCAATCCA TTGGGCTCCC CGTCGGCCAA 
CATCTCATGA TGCGACTCCC TGACCCTGCT AAACCCACCG AATCTATCAT CCGCGCTTAC 
ACGCCCATCT CGGACGGCAC TTTGGAGCGG GGAACTCTCC GGGTGTTGGT CAAGATCTAC 
TACGCTTCTC CTACCGAAGA TATAAAGGGA GGACAAATGA CACAGGCGCT GGATGCTTTG 
GCACTGGGGA AAGCCGTGGA GTTCAAAGGA CCGGTGGGCA AGTTTGTCTA CCAAGGCCGG 
GGCGTCTGCT CTGTCAATGG ACGAGAGAGG AAGGTCAAGA GGTTTGTCAT GGTTTGTGGT 
GGGTCGGGCG TGACGCCTAT CTACCAGGTG CTGAGGGCAG TTGCCGTGGA TGACCAAGAC 
GGGACGGAGT GTTTGGTGTT GGATGGGAAT CGGGTAGAAG GAGACATTCT GATGAAGAGC 
GAGTTGGATG AGTTGGTGGA GAGGGCCAAA CCCGAGGGGA GGTGTAGGGT CAAGTATACT 
TTGAGTAGAC CGGGTGCAGA GTGGGAAGGG CTGAGAGGAA GGCTGGATAA GACGATGCTG 
GAAAGAGAGG TTGGAGAAGG GGATTTGAGG GGGGAGACGA TGGTGTTGCT GTGTGGGCCG 
GAGGGAATGC AGAATATGGT TCGGGAGGTG TTGAAGGGGA TGGGGTGGAA GGATGAGGAT 
GTATTAGTTT TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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nit-3 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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