NCU08132 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU08132
Entrez Gene ID	3875813
Full Name	alpha-1,3-glucan synthase Ags2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_954582.3	XP_959675.3	alpha-1,3-glucan synthase Ags2

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_09639	XP_003721444.1
Neurospora crassa	NCU08132	XP_959675.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU08132 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU08132 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf11137	XM_954582.3 Latest version!	Neurospora crassa OR74A alpha-1,3-glucan synthase Ags2 (NCU08132), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU08132 ( XM_954582.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_959675.3 Neurospora crassa OR74A alpha-1,3-glucan synthase Ags2 (NCU08132), mRNA.

Price & Availability↑

CloneID	ONf11137
Clone ID Related Accession (Same CDS sequence)	XM_954582.3
Accession Version	XM_954582.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	alpha-1,3-glucan synthase Ags2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507). On Feb 23, 2015 this sequence version replaced XM_954582.2.

ATGACTCGTC TAGCGTTGTT TTCGATTCGC GCTCTCTTCA CAATAGCGAC AACATTCACA 
CTCACTGACG CCCTCCGATA CGATGCCCAA GAGATAGAAT ACAATCTCAA CCGGAACAAA 
ACCGCCACAA GTCCTCTTGA CTACTGGGGC GACCGAACAG AGGATGACCC GAACTTTACA 
TATTTCCCTT CGCCCAAAAA CTGGCGAGTA CCCTTCTACA CCGTCTTCGT TGATCGTTGG 
GTAAACGGCG AGCCAGACAA CGACAATGCC AATGGCACGC TTTTCGAGGC CGATATCATG 
AGCAATCAGC TCCGATTCGG CGGTGACGTG GACGGAATCA TCGACTCGCT GGATTACATT 
CAGGGACAGG GAGTGAAGGG AATCTACATT GCAGGCTCCC CCTTTATCAA CCAACCGTGG 
GGTGCGGATT CGTACTCGCC ACTTGACTTG ACCCTTCTTG ATAAGCATTT CGGTAATATC 
AAAAAATGGC AGGAGGCGAT CGACGCCATT CACGACCATG GCATGTGGGT CATCATGGAT 
AACACTATGG CAACCATGGG TGATCTCATT GGTTTCGAGG GTTACCTGAA CACAAGTACG 
CCTTTCCTCG TGGAGGAACA CAAGGTTTTG TGGAAGAGCG ATCGGCGTTA TCTGGACTTC 
GACATCGGCA ACACATACAA CAAGACGTGC GACTATCCAG AGTTTTGGTA CGAAGATGGC 
AAGCGGATCA AGCCTGATGG GCTCAAGGGA TGCTACGACA GCGACTTCGA TCAATATGGT 
GATATCGAAG CCTTCGGTGT TTTCCCTGAT TGGCAACGTC AGCTTGCCAA GTTCGCCTCC 
GTCCAGGATC GTCTCCGCGA ATGGAAGCCC GACGTGATTG CTCGACTCGC CCGATTTTCC 
TGCATGACCA TCGGCATGTT GGACGTCGAC GGTTTCCGTA TCGACAAGGC CGTCCAGGTT 
ACGGTTGATG CTCAGGCGCA TTTTAGCAGC GCGATGCGAA AGTGCGCAAA GGAAAAGTTC 
AACAAGGACA ACTTTATCGT CGTCGGAGAA ATCACAAGTG GTAACACTCT TGGATCTATC 
TACCTTGGTC GCGGTCGCGA GCCTGAACCA GCCGAGGAGC TTTCGGATGA CCCCGTAAAG 
GCTCTGACCC TCGGCAACGC GACCACCGAT ACTTCCAAGT ACTTCGTTCG CGAGAAGGGC 
AACAGTGCCC TGGATGCTGG CGCGTTCCAT TATTCCGTCT ATCGTTTCAT GACCCGCTTC 
TTGGGCATGA GCGGTAACCT CAGAGCCGGC TACGATTTGC CCACGGACTG GATCGAAACC 
TGGCACGAAA TGATCGCAAC AAACGACTTC TGGAATGCCA ACACCGGTGA ATTCGATCCT 
CGCCATCTCT ACGGTACATC CAACCAGGAC GTGTTCCGTT GGCCAGCTAT CGAAAAGGGT 
ACCGAGCGTA TGCTTCTCGC CTACTTCATC ACCACCTTCC TCTTGCCAGG CGCCCCCCTC 
ATGTACTACG GTGAAGAACA GGAGCTATAT GCGCTGGACG GCACGGCCGA AAATTACGTC 
TACGGTCGTC AGGCTATGGC TCCCAGCCCA GCATGGATGG CCCACGGCTG TTTCTCGTTG 
GAAAACGATC TTTACGTCGA TTGGCCCGTC ACCAAGGCCA AAGATGGCTG CAAAGATAAG 
TCCGTGGGCT GGGATCACCG CAATCCTGCT GCGCCCGTGC GCAACATCTT GAAGAACATG 
TTCGCCATGC GCAACTCTTC CCAACTTGGT GTCCTGGAAC ACGGCTGGCT GCTTGAAAAG 
CTTTCCAATC AGACCGATTT CACGTTGCTC AAGGGCAGCG GTGACCCTAC GGAACGTGGC 
ATCTGGAGCG TCGTCCGTGG ACTGTTCCCT GGAGTGCAAG ACAAGCTCAA GGACAAACCT 
GTCTGGTTTG TTTATCATAA CCGAGAGAAC ACAACGACGT ACAAGTTTAA CTGCGACCAG 
AGGGAGGGCG CGTTCCTCTC GCCCTTTGAT GCCGGAGCCA AGGTGAAGAA CCTGATGGAC 
GATGGCGATG TTATTACACT GTCTTCGTCC AATGTCAAGA ACAACTATAC CAACTCGGGC 
AAGAACATGG GCTGTCTTTC AGAGGTCACC TTGAACCCAT TCGAGTTCCG TGTCTACGTG 
GATGACCCTG AGGACCGCTG GATGCAGCCG GCACCGATGA TCACCAAATT TACGCCAGGA 
CACGACGTGC CTATTGACTC GACAAAAGCA AACGGCCGGA TTGACATCTC GTTCGAGTTC 
AATACTGAGA TGGACTGCGA TAGCGTGACT AAGGCGCTCA CTGTCAACTT GACGCAAGAT 
GGCGTGAATG ACCCCACGCA GCTCTCTTGG ACCAAGCCCA ACTGCGAGCT CTTTACACCC 
CAAGATGCAG ACAAAATCAA GTATACGGGA GCAATCAACT CGGCCTGGAG GTGGAATGCT 
ACTTTGACTA ATGTTGCTGA CGGCATCATC AAGCTCACTC TCGATCCTAC CGCCGCAGCG 
AAGGGCGGCA AGGTGACGAA AGGCACTCGC GATCACTTCT TGCTCAGACT TGGCAAGCCT 
TCCAATCCGG TTGTGTGGGG AATGGATGCA ACCTACAACT GGGACCTTCT TCAGAGCGTA 
AGCAACAACT TGTACAGGAT CAAGCATAAC GCCCCTGGCG CAACGAAATG GCGTTACAGC 
ACCAACTTTG GATCTACGTG GAGCAAGTGG GTTGATTATG ACGGCAAAGA GACTTCGGTT 
ACCGGCCAGC AGTGGTCGGG AACTGCTCTG CAGGCCTGGG AGGGCAAGCA TATTCAAGTT 
CAATACTTCA GCAAACCTTT GGGCTCATCG GCGTTTATTC AGCACTCAGA CAAGGGTATC 
GACTACCAGC GTGTGTTCCC TCACATCAAG CTTCACGGTC CCTTCAACAA GTGGGGTTAC 
GACACCGGCC TGCCGGGCTC GATGGATCTC GTCGGTCACA ACACTTGGTC GTTGCATTAC 
ATGTATGAAT GGCCGGCCAA CTTCCAGCTC AACATCTGGG GTATCAACCC GGACAACCAG 
CCTGATGTCT CCTTCATCTT TGGCGATATT GATGGTGACC ACGTTGTGGA CCGCCTTCCC 
CCTAGCAGTC TTGCCAACAA CGTCATCAAC ATCACCGATC CTCCCCCGAC GCCAGCTCTT 
GCGTGGAAAG TTTTCTACAA TGACGCGACC TGGACGTACC ATTTGGAGCC GGCTGGCAAT 
ATGAACGTCC AGATTGCTCT CTTCTGGCTC TTCGCAATCC TTCCTGTGGC GCTGGCATGC 
TTGGCTGGCT GGATCTACGT CACCAGTTTC TACCAAGTCA AGGTCAACAA GTCTGGTTTC 
GCGACCAAGG GCTGGATGCC ACTCAAGATT GGCAACCTCT CGAAGCTCAA CATCGCTAAC 
GTCGGCAAGG GCGTTGAGAT GAACGCCATC ACCCCACCGC CCCCGAGCAG TGCGCTTTCT 
CCCCTTGGTG CTGCCGGTGG CCGGAGAACC GTGTTGATTG CCACCATGGA GTACAACATT 
GATGACTTCA AGATCAAGAT CAAGATTGGT GGTTTGGGTG TCATGGCCCA GCTGATGGCG 
AACGCGTTGC ACCATATGGA CATCGTGTGG GTCATTCCTG TGGTCGGCGA CGTTGACTAT 
CCTCTTGAGA AGATGCAAAA GGCCGAGCCC ATGTACGTCG ATGTTATGGG CCAACCCTAT 
GAGATCGAGG TCTACTACTA TGTCGTCAAG AACATCACCT ACGTCCTTCT CGATGCTCCG 
ATCTTCCGCA AGCAGACTAA GGCCGATCCT TACATTGCCC GTATGGACGA TATTGAAAGT 
GCCATCTTGT ACGCTGCATG GAACTACTGT ATCGCCGAGT CCATCCGCCG CTTCCCGGTC 
GATATTTACC ATATCAACGA TTACCACGGT GCCGCCGCTC CCCTCTATCT GTTGCCCCAG 
ACTATCCCCG TGTGTCTTTC GCTCCACAAC GCCGAATTCC AGGGTATGTG GCCGATGCGT 
ACCCCCGAGG AAGCGAAGGA AGTCTGCGAG GTGTTCAACC TGCCGCCTGA GGTTGTCAAG 
GATTACGTTC AGTATGGTTC CGTCTTCAAC TTGTTGCACG CTGGCGCCAG TTATCTCCGT 
ATTCACCAGC GTGGTTTCGG TGCTGTCGGT GTGTCCCGCA AGTACGGTGA TCGTTCGTTG 
GCTCGTTACC CTATTTTCTG GAGTTTGAAG AACATTGGCC AGCTTCCCAA CCCCGATCCT 
TCCGATACCG CCGACTGGGA TCCCAGCCAG GATGCCAATG ACCAAGCCAA GGGTGTTGAG 
GTTGACCAGG CAGCTGAGGA GAAGAGAGGA GAGTACCGTC GCCAGGCACA GGAATGGGCT 
GGTCTCGAGG TTGACCCTTC GGCTGAGCTT TTCGTCTTCG TTGGTAGATG GTCTCTTCAG 
AAGGGTGTGG ATCTGATTGC TGATCTGTTC CCCAGCATTT TGGAAAAGTA CCCCAAGACG 
CAGCTCATCG CTGTTGGTCC CGTCATTGAT CTCTACGGTC GCTTCGCTGC CCTCAAGCTG 
GAGAAGCTCA TGCAGAAATA CCCCAAGCGT GTCTTCAGCA AGCCCGAGTT CACTTCGCTT 
CCCCCCTACA TCTTCACCGG TGCTGAGTTT GCCCTTATCC CCTCCCGTGA TGAGCCCTTC 
GGTTTGGTCG CTGTCGAGTT CGGAAGAAAG GGTGCCTTGG GTGTCGGTGC TCGTGTCGGT 
GGTCTCGGTC AAATGCCTGG TTTCTGGTAC ACTGTCGAGT CCATGACGCC CTCCCATTTG 
ATCCAACAGT TCAGACAGGC TATCGAATCC GCCCTTGCCT GCAAGCACAA CAAGAGAGCT 
GTCATGCGTG CATGGTCCGC CAAGCAGCGT TTCCCGGTCG CCCAGTGGGT TAAGCAGCTC 
GACGACTTGT ACAGCGAGTC TATCCGTATC CACATCAAAG AGCAGAAGAA GAAGAAGCTG 
GAAGTCCTGA GCCCGGGCCT TACTGTGCCC GGTGCCTCGT CGCGTGCTAG CTACGCTGAT 
CCTGGCAGCG GCCGTTCGTC TCCTGGTCCT CTCGCCGTTC CCTCCCGTCC CGTTACCCCC 
ACGCTCCGCG ATCTTGCTTC TCCCGATCTC CCGACGCCGG GTGCCCCCTG GGCAGGCGGA 
AGGTCCGAGT CACCGGGAGC CCGCGACTCG ACTGCGAGCT CCATTGCTGG AAACTTCTAC 
TTGCAGCCCG GCGGTGCTCG TGAGAGTACT ATGAGTGTAG ACAGCTTTGC GATCCGTGCC 
CAGAATGGAG GACCCATGTC GCCTGGCCTT GGTCCCGATG GCGGCCTTGC CTTCCCTCGT 
CCTGCGTTCA CGCAAGCCCA CCGCAACAGC AGCTTGCTCA GTCTTCCGGA CGTTGTCGGT 
GACCGCCACG ATCTTAAGCT CCAGAAGGTT GATCAGTTCT TCAACGATAC CAACGGCGAA 
TACTATGCGG AGTTCGAGCA GATGCTTGAG GCCTTGACCG CTAGCAACTC TTCCAGTGAC 
CTCTGCATTG AAACCTTCTT GAAGAGGAGT GAAAAGGAGT GGTTTGCTAG GTACAGAGAC 
GCGAAGCTTG GTCGCCACAG AGACGCCAGC CGTATGGGCC GGGGTGGTAG TGGCAGACCC 
GAGTCGAGAG GCGGGGATCG CAGCGAGTCG GTTGTCAGCC GTGGCCGCCA ACCTATGCGC 
AGCGCTACCC CCAGTGGCCT GGCTAGGTCT GTCTTTGAGG AGTCGCCACC GGGTGGGCGC 
AGCGATTACG ACGATGAGTT CTTGTTGGGT GACGGTTACC AGGCCCCCAC TGGTCTCAAG 
AAGACCCTTT CCGTTCGCAT CGGTGACTGG CCGCTCTACT CGTTCTTCCT CGCTCTTGGT 
CAAATCATCT CTGTCAACTC TTACCAGATC GTCCTGTTGA GCGGCTCGCA AGCCCAGACG 
CCTGAGAAGC TCTACACGGT CGGTGGTGTG TACATTGCTA CATCCCTCAT GTGGTGGGGT 
ATGGAGCGCA ACTTCAAGTC GGTCTACGCC CTGTCTGCTC CCTGGTTCTT CTTCGGTCTC 
GCCTTCCTTC TTCTCGGTGT CGCGCCATTC ATTGGCGACT GGAAGGTGGC TGACAGCGTG 
ACAAGCGCGG CTACCGCCTT TTACGCGGCC GGAGCCAGTC ATGGTGCCTT GTCTTTCGCC 
CTCAACTTTG GTGACGAAGG TGGTGCTCCT ACCAGGCAGT GGATTACTCG TGCCCTGATC 
GTTTCCGGTA TCGCTCAAGT CTACAGTCTT GGTCTTTGGT ATTGGGGCGC GACTGTCTCG 
AGCGAGGCTC AGACCATGAC CAAGTTCATT GGCGAGTCGG GCGTCCCCCT GGCCCTTGTC 
ATTTGCGTAC CGGTTGCCAT CTTCCTTTGG GCGATTGGAG CCATTCTTTA TGTTGGATTG 
CCAGACTTCT ACCGTCAGTC TCCGGCCACG ATTCCCGGCT TCTACATCTC GCTGTACAGG 
CGTAAGATTG TGCCTTGGTT CTTCGTCATG ATCATCCTCC AGAACTACTG GCTGTCGGCG 
CCCTATGGCC GCAGCTGGAG CTTCCTGTTC TCGACGCAGT TCATCCCCAA CTGGGGCATC 
TTCCTGATGG CCCTCGGTTT CTTCGTGGTG CTCTGGAGCA TCGTCCTCTA CGGCTTCTCC 
TACTTTTCGG ACGAACACAC GTGGCTCCTG CCCATCTTCG CCATCGGTCT CTGCGCTCCT 
CGCTGGGCCC AGGAGATGTG GGCCTGCAGC GGCATCGGCT GGTACCTCCC GTGGGCCGGC 
GGCCCCGTCG GCTCGGCCGT CCTCTCCAGG TGTCTCTGGT TGTGGCTGGG CCTGCTGGAT 
AACATCCAGG GCGTCGGTCT CGGTATGATG CTGCTGGCGA CACTGACGAG ACAACACGTG 
TTGGTGGTGT TGATCGGCGC CCAAGTGATC GGTTCCATGT TCACGTTGAT TGGACGCGCG 
ACAAGCCCCA ACGCGCTGTC GCCCAATACG ACTTTCCCCG ATTTCTCGCA GGGCCTGTAT 
CCCGGCATTG CGAGCCCGTA CTTCTGGGTT TGCTTGATCT TCCAGCTGAT CATTCCAGTT 
GGATTCTTCA AGTTCTTCAG AAAGGAACAG GTGGCGAAGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_959675.3
CDS	869..7993
Translation	MTRLALFSIRALFTIATTFTLTDALRYDAQEIEYNLNRNKTATSPLDYWGDRTEDDPNFTYFPSPKNWRVPFYTVFVDRWVNGEPDNDNANGTLFEADIMSNQLRFGGDVDGIIDSLDYIQGQGVKGIYIAGSPFINQPWGADSYSPLDLTLLDKHFGNIKKWQEAIDAIHDHGMWVIMDNTMATMGDLIGFEGYLNTSTPFLVEEHKVLWKSDRRYLDFDIGNTYNKTCDYPEFWYEDGKRIKPDGLKGCYDSDFDQYGDIEAFGVFPDWQRQLAKFASVQDRLREWKPDVIARLARFSCMTIGMLDVDGFRIDKAVQVTVDAQAHFSSAMRKCAKEKFNKDNFIVVGEITSGNTLGSIYLGRGREPEPAEELSDDPVKALTLGNATTDTSKYFVREKGNSALDAGAFHYSVYRFMTRFLGMSGNLRAGYDLPTDWIETWHEMIATNDFWNANTGEFDPRHLYGTSNQDVFRWPAIEKGTERMLLAYFITTFLLPGAPLMYYGEEQELYALDGTAENYVYGRQAMAPSPAWMAHGCFSLENDLYVDWPVTKAKDGCKDKSVGWDHRNPAAPVRNILKNMFAMRNSSQLGVLEHGWLLEKLSNQTDFTLLKGSGDPTERGIWSVVRGLFPGVQDKLKDKPVWFVYHNRENTTTYKFNCDQREGAFLSPFDAGAKVKNLMDDGDVITLSSSNVKNNYTNSGKNMGCLSEVTLNPFEFRVYVDDPEDRWMQPAPMITKFTPGHDVPIDSTKANGRIDISFEFNTEMDCDSVTKALTVNLTQDGVNDPTQLSWTKPNCELFTPQDADKIKYTGAINSAWRWNATLTNVADGIIKLTLDPTAAAKGGKVTKGTRDHFLLRLGKPSNPVVWGMDATYNWDLLQSVSNNLYRIKHNAPGATKWRYSTNFGSTWSKWVDYDGKETSVTGQQWSGTALQAWEGKHIQVQYFSKPLGSSAFIQHSDKGIDYQRVFPHIKLHGPFNKWGYDTGLPGSMDLVGHNTWSLHYMYEWPANFQLNIWGINPDNQPDVSFIFGDIDGDHVVDRLPPSSLANNVINITDPPPTPALAWKVFYNDATWTYHLEPAGNMNVQIALFWLFAILPVALACLAGWIYVTSFYQVKVNKSGFATKGWMPLKIGNLSKLNIANVGKGVEMNAITPPPPSSALSPLGAAGGRRTVLIATMEYNIDDFKIKIKIGGLGVMAQLMANALHHMDIVWVIPVVGDVDYPLEKMQKAEPMYVDVMGQPYEIEVYYYVVKNITYVLLDAPIFRKQTKADPYIARMDDIESAILYAAWNYCIAESIRRFPVDIYHINDYHGAAAPLYLLPQTIPVCLSLHNAEFQGMWPMRTPEEAKEVCEVFNLPPEVVKDYVQYGSVFNLLHAGASYLRIHQRGFGAVGVSRKYGDRSLARYPIFWSLKNIGQLPNPDPSDTADWDPSQDANDQAKGVEVDQAAEEKRGEYRRQAQEWAGLEVDPSAELFVFVGRWSLQKGVDLIADLFPSILEKYPKTQLIAVGPVIDLYGRFAALKLEKLMQKYPKRVFSKPEFTSLPPYIFTGAEFALIPSRDEPFGLVAVEFGRKGALGVGARVGGLGQMPGFWYTVESMTPSHLIQQFRQAIESALACKHNKRAVMRAWSAKQRFPVAQWVKQLDDLYSESIRIHIKEQKKKKLEVLSPGLTVPGASSRASYADPGSGRSSPGPLAVPSRPVTPTLRDLASPDLPTPGAPWAGGRSESPGARDSTASSIAGNFYLQPGGARESTMSVDSFAIRAQNGGPMSPGLGPDGGLAFPRPAFTQAHRNSSLLSLPDVVGDRHDLKLQKVDQFFNDTNGEYYAEFEQMLEALTASNSSSDLCIETFLKRSEKEWFARYRDAKLGRHRDASRMGRGGSGRPESRGGDRSESVVSRGRQPMRSATPSGLARSVFEESPPGGRSDYDDEFLLGDGYQAPTGLKKTLSVRIGDWPLYSFFLALGQIISVNSYQIVLLSGSQAQTPEKLYTVGGVYIATSLMWWGMERNFKSVYALSAPWFFFGLAFLLLGVAPFIGDWKVADSVTSAATAFYAAGASHGALSFALNFGDEGGAPTRQWITRALIVSGIAQVYSLGLWYWGATVSSEAQTMTKFIGESGVPLALVICVPVAIFLWAIGAILYVGLPDFYRQSPATIPGFYISLYRRKIVPWFFVMIILQNYWLSAPYGRSWSFLFSTQFIPNWGIFLMALGFFVVLWSIVLYGFSYFSDEHTWLLPIFAIGLCAPRWAQEMWACSGIGWYLPWAGGPVGSAVLSRCLWLWLGLLDNIQGVGLGMMLLATLTRQHVLVVLIGAQVIGSMFTLIGRATSPNALSPNTTFPDFSQGLYPGIASPYFWVCLIFQLIIPVGFFKFFRKEQVAKP

Target ORF information:

RefSeq Version	XM_954582.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A alpha-1,3-glucan synthase Ags2 (NCU08132), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_954582.3

ORF Insert Sequence:

ATGACTCGTC TAGCGTTGTT TTCGATTCGC GCTCTCTTCA CAATAGCGAC AACATTCACA 
CTCACTGACG CCCTCCGATA CGATGCCCAA GAGATAGAAT ACAATCTCAA CCGGAACAAA 
ACCGCCACAA GTCCTCTTGA CTACTGGGGC GACCGAACAG AGGATGACCC GAACTTTACA 
TATTTCCCTT CGCCCAAAAA CTGGCGAGTA CCCTTCTACA CCGTCTTCGT TGATCGTTGG 
GTAAACGGCG AGCCAGACAA CGACAATGCC AATGGCACGC TTTTCGAGGC CGATATCATG 
AGCAATCAGC TCCGATTCGG CGGTGACGTG GACGGAATCA TCGACTCGCT GGATTACATT 
CAGGGACAGG GAGTGAAGGG AATCTACATT GCAGGCTCCC CCTTTATCAA CCAACCGTGG 
GGTGCGGATT CGTACTCGCC ACTTGACTTG ACCCTTCTTG ATAAGCATTT CGGTAATATC 
AAAAAATGGC AGGAGGCGAT CGACGCCATT CACGACCATG GCATGTGGGT CATCATGGAT 
AACACTATGG CAACCATGGG TGATCTCATT GGTTTCGAGG GTTACCTGAA CACAAGTACG 
CCTTTCCTCG TGGAGGAACA CAAGGTTTTG TGGAAGAGCG ATCGGCGTTA TCTGGACTTC 
GACATCGGCA ACACATACAA CAAGACGTGC GACTATCCAG AGTTTTGGTA CGAAGATGGC 
AAGCGGATCA AGCCTGATGG GCTCAAGGGA TGCTACGACA GCGACTTCGA TCAATATGGT 
GATATCGAAG CCTTCGGTGT TTTCCCTGAT TGGCAACGTC AGCTTGCCAA GTTCGCCTCC 
GTCCAGGATC GTCTCCGCGA ATGGAAGCCC GACGTGATTG CTCGACTCGC CCGATTTTCC 
TGCATGACCA TCGGCATGTT GGACGTCGAC GGTTTCCGTA TCGACAAGGC CGTCCAGGTT 
ACGGTTGATG CTCAGGCGCA TTTTAGCAGC GCGATGCGAA AGTGCGCAAA GGAAAAGTTC 
AACAAGGACA ACTTTATCGT CGTCGGAGAA ATCACAAGTG GTAACACTCT TGGATCTATC 
TACCTTGGTC GCGGTCGCGA GCCTGAACCA GCCGAGGAGC TTTCGGATGA CCCCGTAAAG 
GCTCTGACCC TCGGCAACGC GACCACCGAT ACTTCCAAGT ACTTCGTTCG CGAGAAGGGC 
AACAGTGCCC TGGATGCTGG CGCGTTCCAT TATTCCGTCT ATCGTTTCAT GACCCGCTTC 
TTGGGCATGA GCGGTAACCT CAGAGCCGGC TACGATTTGC CCACGGACTG GATCGAAACC 
TGGCACGAAA TGATCGCAAC AAACGACTTC TGGAATGCCA ACACCGGTGA ATTCGATCCT 
CGCCATCTCT ACGGTACATC CAACCAGGAC GTGTTCCGTT GGCCAGCTAT CGAAAAGGGT 
ACCGAGCGTA TGCTTCTCGC CTACTTCATC ACCACCTTCC TCTTGCCAGG CGCCCCCCTC 
ATGTACTACG GTGAAGAACA GGAGCTATAT GCGCTGGACG GCACGGCCGA AAATTACGTC 
TACGGTCGTC AGGCTATGGC TCCCAGCCCA GCATGGATGG CCCACGGCTG TTTCTCGTTG 
GAAAACGATC TTTACGTCGA TTGGCCCGTC ACCAAGGCCA AAGATGGCTG CAAAGATAAG 
TCCGTGGGCT GGGATCACCG CAATCCTGCT GCGCCCGTGC GCAACATCTT GAAGAACATG 
TTCGCCATGC GCAACTCTTC CCAACTTGGT GTCCTGGAAC ACGGCTGGCT GCTTGAAAAG 
CTTTCCAATC AGACCGATTT CACGTTGCTC AAGGGCAGCG GTGACCCTAC GGAACGTGGC 
ATCTGGAGCG TCGTCCGTGG ACTGTTCCCT GGAGTGCAAG ACAAGCTCAA GGACAAACCT 
GTCTGGTTTG TTTATCATAA CCGAGAGAAC ACAACGACGT ACAAGTTTAA CTGCGACCAG 
AGGGAGGGCG CGTTCCTCTC GCCCTTTGAT GCCGGAGCCA AGGTGAAGAA CCTGATGGAC 
GATGGCGATG TTATTACACT GTCTTCGTCC AATGTCAAGA ACAACTATAC CAACTCGGGC 
AAGAACATGG GCTGTCTTTC AGAGGTCACC TTGAACCCAT TCGAGTTCCG TGTCTACGTG 
GATGACCCTG AGGACCGCTG GATGCAGCCG GCACCGATGA TCACCAAATT TACGCCAGGA 
CACGACGTGC CTATTGACTC GACAAAAGCA AACGGCCGGA TTGACATCTC GTTCGAGTTC 
AATACTGAGA TGGACTGCGA TAGCGTGACT AAGGCGCTCA CTGTCAACTT GACGCAAGAT 
GGCGTGAATG ACCCCACGCA GCTCTCTTGG ACCAAGCCCA ACTGCGAGCT CTTTACACCC 
CAAGATGCAG ACAAAATCAA GTATACGGGA GCAATCAACT CGGCCTGGAG GTGGAATGCT 
ACTTTGACTA ATGTTGCTGA CGGCATCATC AAGCTCACTC TCGATCCTAC CGCCGCAGCG 
AAGGGCGGCA AGGTGACGAA AGGCACTCGC GATCACTTCT TGCTCAGACT TGGCAAGCCT 
TCCAATCCGG TTGTGTGGGG AATGGATGCA ACCTACAACT GGGACCTTCT TCAGAGCGTA 
AGCAACAACT TGTACAGGAT CAAGCATAAC GCCCCTGGCG CAACGAAATG GCGTTACAGC 
ACCAACTTTG GATCTACGTG GAGCAAGTGG GTTGATTATG ACGGCAAAGA GACTTCGGTT 
ACCGGCCAGC AGTGGTCGGG AACTGCTCTG CAGGCCTGGG AGGGCAAGCA TATTCAAGTT 
CAATACTTCA GCAAACCTTT GGGCTCATCG GCGTTTATTC AGCACTCAGA CAAGGGTATC 
GACTACCAGC GTGTGTTCCC TCACATCAAG CTTCACGGTC CCTTCAACAA GTGGGGTTAC 
GACACCGGCC TGCCGGGCTC GATGGATCTC GTCGGTCACA ACACTTGGTC GTTGCATTAC 
ATGTATGAAT GGCCGGCCAA CTTCCAGCTC AACATCTGGG GTATCAACCC GGACAACCAG 
CCTGATGTCT CCTTCATCTT TGGCGATATT GATGGTGACC ACGTTGTGGA CCGCCTTCCC 
CCTAGCAGTC TTGCCAACAA CGTCATCAAC ATCACCGATC CTCCCCCGAC GCCAGCTCTT 
GCGTGGAAAG TTTTCTACAA TGACGCGACC TGGACGTACC ATTTGGAGCC GGCTGGCAAT 
ATGAACGTCC AGATTGCTCT CTTCTGGCTC TTCGCAATCC TTCCTGTGGC GCTGGCATGC 
TTGGCTGGCT GGATCTACGT CACCAGTTTC TACCAAGTCA AGGTCAACAA GTCTGGTTTC 
GCGACCAAGG GCTGGATGCC ACTCAAGATT GGCAACCTCT CGAAGCTCAA CATCGCTAAC 
GTCGGCAAGG GCGTTGAGAT GAACGCCATC ACCCCACCGC CCCCGAGCAG TGCGCTTTCT 
CCCCTTGGTG CTGCCGGTGG CCGGAGAACC GTGTTGATTG CCACCATGGA GTACAACATT 
GATGACTTCA AGATCAAGAT CAAGATTGGT GGTTTGGGTG TCATGGCCCA GCTGATGGCG 
AACGCGTTGC ACCATATGGA CATCGTGTGG GTCATTCCTG TGGTCGGCGA CGTTGACTAT 
CCTCTTGAGA AGATGCAAAA GGCCGAGCCC ATGTACGTCG ATGTTATGGG CCAACCCTAT 
GAGATCGAGG TCTACTACTA TGTCGTCAAG AACATCACCT ACGTCCTTCT CGATGCTCCG 
ATCTTCCGCA AGCAGACTAA GGCCGATCCT TACATTGCCC GTATGGACGA TATTGAAAGT 
GCCATCTTGT ACGCTGCATG GAACTACTGT ATCGCCGAGT CCATCCGCCG CTTCCCGGTC 
GATATTTACC ATATCAACGA TTACCACGGT GCCGCCGCTC CCCTCTATCT GTTGCCCCAG 
ACTATCCCCG TGTGTCTTTC GCTCCACAAC GCCGAATTCC AGGGTATGTG GCCGATGCGT 
ACCCCCGAGG AAGCGAAGGA AGTCTGCGAG GTGTTCAACC TGCCGCCTGA GGTTGTCAAG 
GATTACGTTC AGTATGGTTC CGTCTTCAAC TTGTTGCACG CTGGCGCCAG TTATCTCCGT 
ATTCACCAGC GTGGTTTCGG TGCTGTCGGT GTGTCCCGCA AGTACGGTGA TCGTTCGTTG 
GCTCGTTACC CTATTTTCTG GAGTTTGAAG AACATTGGCC AGCTTCCCAA CCCCGATCCT 
TCCGATACCG CCGACTGGGA TCCCAGCCAG GATGCCAATG ACCAAGCCAA GGGTGTTGAG 
GTTGACCAGG CAGCTGAGGA GAAGAGAGGA GAGTACCGTC GCCAGGCACA GGAATGGGCT 
GGTCTCGAGG TTGACCCTTC GGCTGAGCTT TTCGTCTTCG TTGGTAGATG GTCTCTTCAG 
AAGGGTGTGG ATCTGATTGC TGATCTGTTC CCCAGCATTT TGGAAAAGTA CCCCAAGACG 
CAGCTCATCG CTGTTGGTCC CGTCATTGAT CTCTACGGTC GCTTCGCTGC CCTCAAGCTG 
GAGAAGCTCA TGCAGAAATA CCCCAAGCGT GTCTTCAGCA AGCCCGAGTT CACTTCGCTT 
CCCCCCTACA TCTTCACCGG TGCTGAGTTT GCCCTTATCC CCTCCCGTGA TGAGCCCTTC 
GGTTTGGTCG CTGTCGAGTT CGGAAGAAAG GGTGCCTTGG GTGTCGGTGC TCGTGTCGGT 
GGTCTCGGTC AAATGCCTGG TTTCTGGTAC ACTGTCGAGT CCATGACGCC CTCCCATTTG 
ATCCAACAGT TCAGACAGGC TATCGAATCC GCCCTTGCCT GCAAGCACAA CAAGAGAGCT 
GTCATGCGTG CATGGTCCGC CAAGCAGCGT TTCCCGGTCG CCCAGTGGGT TAAGCAGCTC 
GACGACTTGT ACAGCGAGTC TATCCGTATC CACATCAAAG AGCAGAAGAA GAAGAAGCTG 
GAAGTCCTGA GCCCGGGCCT TACTGTGCCC GGTGCCTCGT CGCGTGCTAG CTACGCTGAT 
CCTGGCAGCG GCCGTTCGTC TCCTGGTCCT CTCGCCGTTC CCTCCCGTCC CGTTACCCCC 
ACGCTCCGCG ATCTTGCTTC TCCCGATCTC CCGACGCCGG GTGCCCCCTG GGCAGGCGGA 
AGGTCCGAGT CACCGGGAGC CCGCGACTCG ACTGCGAGCT CCATTGCTGG AAACTTCTAC 
TTGCAGCCCG GCGGTGCTCG TGAGAGTACT ATGAGTGTAG ACAGCTTTGC GATCCGTGCC 
CAGAATGGAG GACCCATGTC GCCTGGCCTT GGTCCCGATG GCGGCCTTGC CTTCCCTCGT 
CCTGCGTTCA CGCAAGCCCA CCGCAACAGC AGCTTGCTCA GTCTTCCGGA CGTTGTCGGT 
GACCGCCACG ATCTTAAGCT CCAGAAGGTT GATCAGTTCT TCAACGATAC CAACGGCGAA 
TACTATGCGG AGTTCGAGCA GATGCTTGAG GCCTTGACCG CTAGCAACTC TTCCAGTGAC 
CTCTGCATTG AAACCTTCTT GAAGAGGAGT GAAAAGGAGT GGTTTGCTAG GTACAGAGAC 
GCGAAGCTTG GTCGCCACAG AGACGCCAGC CGTATGGGCC GGGGTGGTAG TGGCAGACCC 
GAGTCGAGAG GCGGGGATCG CAGCGAGTCG GTTGTCAGCC GTGGCCGCCA ACCTATGCGC 
AGCGCTACCC CCAGTGGCCT GGCTAGGTCT GTCTTTGAGG AGTCGCCACC GGGTGGGCGC 
AGCGATTACG ACGATGAGTT CTTGTTGGGT GACGGTTACC AGGCCCCCAC TGGTCTCAAG 
AAGACCCTTT CCGTTCGCAT CGGTGACTGG CCGCTCTACT CGTTCTTCCT CGCTCTTGGT 
CAAATCATCT CTGTCAACTC TTACCAGATC GTCCTGTTGA GCGGCTCGCA AGCCCAGACG 
CCTGAGAAGC TCTACACGGT CGGTGGTGTG TACATTGCTA CATCCCTCAT GTGGTGGGGT 
ATGGAGCGCA ACTTCAAGTC GGTCTACGCC CTGTCTGCTC CCTGGTTCTT CTTCGGTCTC 
GCCTTCCTTC TTCTCGGTGT CGCGCCATTC ATTGGCGACT GGAAGGTGGC TGACAGCGTG 
ACAAGCGCGG CTACCGCCTT TTACGCGGCC GGAGCCAGTC ATGGTGCCTT GTCTTTCGCC 
CTCAACTTTG GTGACGAAGG TGGTGCTCCT ACCAGGCAGT GGATTACTCG TGCCCTGATC 
GTTTCCGGTA TCGCTCAAGT CTACAGTCTT GGTCTTTGGT ATTGGGGCGC GACTGTCTCG 
AGCGAGGCTC AGACCATGAC CAAGTTCATT GGCGAGTCGG GCGTCCCCCT GGCCCTTGTC 
ATTTGCGTAC CGGTTGCCAT CTTCCTTTGG GCGATTGGAG CCATTCTTTA TGTTGGATTG 
CCAGACTTCT ACCGTCAGTC TCCGGCCACG ATTCCCGGCT TCTACATCTC GCTGTACAGG 
CGTAAGATTG TGCCTTGGTT CTTCGTCATG ATCATCCTCC AGAACTACTG GCTGTCGGCG 
CCCTATGGCC GCAGCTGGAG CTTCCTGTTC TCGACGCAGT TCATCCCCAA CTGGGGCATC 
TTCCTGATGG CCCTCGGTTT CTTCGTGGTG CTCTGGAGCA TCGTCCTCTA CGGCTTCTCC 
TACTTTTCGG ACGAACACAC GTGGCTCCTG CCCATCTTCG CCATCGGTCT CTGCGCTCCT 
CGCTGGGCCC AGGAGATGTG GGCCTGCAGC GGCATCGGCT GGTACCTCCC GTGGGCCGGC 
GGCCCCGTCG GCTCGGCCGT CCTCTCCAGG TGTCTCTGGT TGTGGCTGGG CCTGCTGGAT 
AACATCCAGG GCGTCGGTCT CGGTATGATG CTGCTGGCGA CACTGACGAG ACAACACGTG 
TTGGTGGTGT TGATCGGCGC CCAAGTGATC GGTTCCATGT TCACGTTGAT TGGACGCGCG 
ACAAGCCCCA ACGCGCTGTC GCCCAATACG ACTTTCCCCG ATTTCTCGCA GGGCCTGTAT 
CCCGGCATTG CGAGCCCGTA CTTCTGGGTT TGCTTGATCT TCCAGCTGAT CATTCCAGTT 
GGATTCTTCA AGTTCTTCAG AAAGGAACAG GTGGCGAAGC CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU08132 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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