NCU09552 cDNA ORF clone, Neurospora crassa OR74A

The following NCU09552 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU09552 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ONf00722 XM_953678.2
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Neurospora crassa OR74A topoisomerase 1-associated factor 1 (NCU09552), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf00722
    Clone ID Related Accession (Same CDS sequence) XM_953678.2
    Accession Version XM_953678.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product topoisomerase 1-associated factor 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505). On Feb 23, 2015 this sequence version replaced XM_953678.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGCCG CAGACATCAA CGACGATGTC GTCCATCCGG AAGTGCGGGC GCACATCACC 
    AACCTGGTTT CTGCGCTCGG TGGTTACAGC GCGGACGATG ATGGAAGCTA CAAGCTCGGT
    GACGAAGCGC TAGACGTCTT GCGCGACCTG AAGAAATGGA TCCGATTCTA CGATGAAAAG
    ACCAATCGCA TGGACGTCGC CAGATGTCTG GCTGAAGCGA ACCTCGTCGG CGGCGATCTG
    CTCCAGATCC TGACATTATG GCCTCAAAGC GAAACCGACA GCAAGTACAA GGCCCGTATT
    GCTCTGGCGT GTTTCGAGGT CATGGTGCCC CTTACTTGGC CCATAGAGAA GGAGCGCGCC
    GAAATGACCA TCAACCACCA TCGTCACATG CCCGTTCTGC AGCTTGCCCA GCTTGGCTAC
    AAGCGCGCCA TCATAAACTT CGATGCGATA CCCATCCTCA ACACCGCCGT CAGGGTAGCC
    CTTCCCTCCA TGGCCATGCC TATCGGCGAG CGGACACCCC GTGACCAGGC CATCATCAAG
    CTCATTCTCT TCTTTTTGAG GAACGTTGCC ATGATCGCAC CCCCGCAAGG TGTCAAATGC
    GAAGGCGACG AGACACAGGT ATCCAGGTCG GCAACCATTG ATGCCTTTTC GTATCAAGAC
    ATATTTCTCA CTCTGCTGAC CCTTGCCTCC AACATGGGCG AGGACTTCCG CACCGAAGAT
    GTAGTTATCA TGGAAATCAT CTTTCATCTT GTAAAAAGAG TTGACCCCAG CTCGCTCTTC
    GTCAGTGAAA AGCAACTCAA CAAAGCCAAG GGGCAAGAGC TGGCATCGGA GATGCGCAAG
    GAGGCTGCTA TGCTAAAATC CTACAACAAA ACCACCACCA CTCGGCACAG TCGATTTGGG
    ACCATGATCT GGGTGAAACG AGCAGATGGC AAGATGGTGA CGGTATCTGG CCAAGAGGCT
    CTTTTGGACG CAAAAACGCG AGAGAGGAAG ATGGATAACT CCAAAACATT CAGACCGCCA
    AGAAGAGCGA GGAAACCCGA GATGGAGCCC AAGGACCTTG GCCCTCCGGT GACGCTAGAT
    GAAAGAGCTC GTCAGCAGCT GCGATCATTC GTCCAAGACT TCCTCGACTC CGGTTTCAAC
    CCCTTGTTTC TGCACGTGAG GCAATCGATC GATCGGGAGG CATTGCATGC ATTGAACCAG
    CATAAGAGCC AGTTCTTTTA CCTTGTCGCC TGGTTCCTCG AAGCCGAGCG GATGCGGAGA
    AAGGCCAAGA GGGACGAGTC GAAGTCGACG TCCGCAGCCG GAGAGGAGGT GAACAGTTTT
    AACCTCGTTG CTGCTGTGCT CCAACAGGAA ATGTTCGCTT CCATGAATCG TGCGCTTGAC
    CGGTCCTATA GTGATAAGGA TTGGCAACTG CTCACGTCCG TCATGCGCTG TTACACCCAG
    ATCTTCCTCA CAGTCCAGGA GATGTCAGAG TCTCCCAACG AGGAAGACCA GGAGATTGCC
    GAAAACACCC TGAGCCGTCT CTTTTACGAG GAGACAACCC ACGACCTTAT CGCGAATATT
    GCTCGAACAT ACAAGGATCA AGGCTTCGAA TATCTTGATG CCGCGACCGA GCTTGTCCAC
    ACTTTCCTCC GCATTTTAGA AGGTTACTCT AAGCAAAATG TCGATTTGCA GGTGCGCTCA
    CGGAAGCGAG CTCGCCGAAA GAAGAAGGCT GCCAAAGCTG CTGCTGCTGT TGCTGCAGCT
    AGAGCCGCCG GTGAGGAAGC AGAAGACGTT GGCGTTCCCG AGGACAATGA TGCCGATGAT
    TCTGGGGACG ATGAGCAACA TGCAGAGCGG GTCACTCAGG AGCGCAAGTT CGAGTTTGGG
    AAATTCGCCA TTCGATTCGC TCCCCAGGGT GTTGTCGACA CATTTGTCGC CTTCACCAAA
    TTCTACCGGG ATTTGAACGA CGCACAGCTG AAGCGCGCTC ATCGCTACTT CTACCGCGTT
    GCCTTCAAAC TCGAGCTGAG CATCATGCTA TTCCGACTAG ACATCATCAA TCTCTTTTAC
    AACATGGTCC AAGGTCCCGA GCCCCTCGAC AAAAGCTCGC CGATGTTCAA GGAATGGGAG
    GAGCTCTCCA AGCAGATCAT TCGGAAATGT GTCAAAAAGC TTCAAGAGCG GCCTGCGTTG
    TTCACCGAGT TGCTCTTTTC CAAGATTGGC AGCACGACGC ACTTCCTTGA GCACGGCTAT
    GAGAAGCCTG TCACCACAAC TACGCCACGA CCTGGTGCCG AGCTGGAGTT CAAGCGAGCC
    ACAGAGCGCG ACGAGCAGAT TGGCATTGCG GTCAGCGTAC TCATCGACAA ACAGCAAGTC
    GAGCATCTGC AGTGGCTCAA GGATCAGCTG ACAAGTGCAA TGAGTGAGCG GCAAGCATGG
    GAGAACGTAG ACAAGGCCAT GGCAGCCACT ACCGAGGGGG CGGCTGATGG TGAAGCTGCC
    GACGAGAGAT CCAACAAATC AGCACCACCA CATATCACGA TCCGCCCTGA TACCGAGGCT
    CGCCGCACAG CCATGTTCAA GAACCCGCAC CTCCGTCTTC TCATGCGTCT AGTCGGTATG
    GAGCGTCTCA CGCCTACTCT CGACGAGACC CCCGACTCGA CCTGGATTCT CCCAGGCAGC
    CACACCGCCG AGGCGATCCA AGATACCATC GACCTCATCA ACAAGGCCGA GTTCTCCCCT
    CCCACCTTTG AGGACGGCGG CTCAGCCGAA GACCAGCTCC GGCGCAAGTC CGCCGCCGCT
    TCTCGCAGGA CGCGTGCCGC ATATGACGAT GATGAGGAAG AAATTCGCGG ATTCCTCGGT
    GACGATGACG ACGAAGACTT TCTCTTTGCG CCCGGCGGTC CTACCGCTCG CAAGCCGGAC
    GCTAGGCCGC AGAAGAAGCG TCAGCGCAAG CGCCGAAGAG AGGCTGGTAG TGGAGACGAG
    GAAGATGAGG GGGTGTCGGA TGAAGTGTTG GCTGCGCGTG CGAAGAAGAG GAGGGAGAAG
    GAGCTGGAGA AGATTAGGAA GATCAAGAGT GAGATGTACG TGCACGCAAG CGACGATGAA
    ACGGACGATG AGAGGGATCG GGAGTTCTTT GAGCGGGAGA GAAAGAGGCA GGAGACGAAG
    GATTCAAAGT TCGATAGCAT GCTTGGGGCG TTGGGGTTGT CAGTGTTGTC GCAGGTGAAT
    GGAGGGGAAA AGAGCGCGTG GGAGGCGGTG CTGGATGATG AGCCCGAGAG TGATGAGAGT
    GAGAATGAAG GGCGGAAGAA TGCGAAGCGG AGGAAGAAGC AGGTCGCTTC TGGTTCCGAA
    GAAGAGCAAG AGGAGGAGGA GGAAGAGGAG GAAGAGGAGG ACAGCGATGA AGAATTGCCA
    ACGAAACAGG CCAAGAGTAA AACCTCGAAA AGGAAGGCGG CGGTACCGTC AAAACGACCG
    GCAAGGAGGC CTGGGACAGC CAAGAAAAGG GCTGTCGTGG AGCTCAGCGA CAATGATGAA
    GATGAAGATG AGGAAGAGGA TGCCATGGAC GTAGATTCGG CAAACGAACG CACGACCAGA
    AATGAGGCAC CCTTACCCTC TAGCCCTGGT GAGGGGCTAG GGAGGAGAAT AGACAAGATG
    GCGATGGATG ATGGAGACGA GGATGAGGAT GATCAGCCGG TGGTCGCTGC TCGCCAACGG
    CCAAAAGCCA GGGGTGGATT CATCATTGAC AGCAGCGATG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_958771.1
    CDS170..3877
    Translation

    Target ORF information:

    RefSeq Version XM_953678.2
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A topoisomerase 1-associated factor 1 (NCU09552), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_953678.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGCCG CAGACATCAA CGACGATGTC GTCCATCCGG AAGTGCGGGC GCACATCACC 
    AACCTGGTTT CTGCGCTCGG TGGTTACAGC GCGGACGATG ATGGAAGCTA CAAGCTCGGT
    GACGAAGCGC TAGACGTCTT GCGCGACCTG AAGAAATGGA TCCGATTCTA CGATGAAAAG
    ACCAATCGCA TGGACGTCGC CAGATGTCTG GCTGAAGCGA ACCTCGTCGG CGGCGATCTG
    CTCCAGATCC TGACATTATG GCCTCAAAGC GAAACCGACA GCAAGTACAA GGCCCGTATT
    GCTCTGGCGT GTTTCGAGGT CATGGTGCCC CTTACTTGGC CCATAGAGAA GGAGCGCGCC
    GAAATGACCA TCAACCACCA TCGTCACATG CCCGTTCTGC AGCTTGCCCA GCTTGGCTAC
    AAGCGCGCCA TCATAAACTT CGATGCGATA CCCATCCTCA ACACCGCCGT CAGGGTAGCC
    CTTCCCTCCA TGGCCATGCC TATCGGCGAG CGGACACCCC GTGACCAGGC CATCATCAAG
    CTCATTCTCT TCTTTTTGAG GAACGTTGCC ATGATCGCAC CCCCGCAAGG TGTCAAATGC
    GAAGGCGACG AGACACAGGT ATCCAGGTCG GCAACCATTG ATGCCTTTTC GTATCAAGAC
    ATATTTCTCA CTCTGCTGAC CCTTGCCTCC AACATGGGCG AGGACTTCCG CACCGAAGAT
    GTAGTTATCA TGGAAATCAT CTTTCATCTT GTAAAAAGAG TTGACCCCAG CTCGCTCTTC
    GTCAGTGAAA AGCAACTCAA CAAAGCCAAG GGGCAAGAGC TGGCATCGGA GATGCGCAAG
    GAGGCTGCTA TGCTAAAATC CTACAACAAA ACCACCACCA CTCGGCACAG TCGATTTGGG
    ACCATGATCT GGGTGAAACG AGCAGATGGC AAGATGGTGA CGGTATCTGG CCAAGAGGCT
    CTTTTGGACG CAAAAACGCG AGAGAGGAAG ATGGATAACT CCAAAACATT CAGACCGCCA
    AGAAGAGCGA GGAAACCCGA GATGGAGCCC AAGGACCTTG GCCCTCCGGT GACGCTAGAT
    GAAAGAGCTC GTCAGCAGCT GCGATCATTC GTCCAAGACT TCCTCGACTC CGGTTTCAAC
    CCCTTGTTTC TGCACGTGAG GCAATCGATC GATCGGGAGG CATTGCATGC ATTGAACCAG
    CATAAGAGCC AGTTCTTTTA CCTTGTCGCC TGGTTCCTCG AAGCCGAGCG GATGCGGAGA
    AAGGCCAAGA GGGACGAGTC GAAGTCGACG TCCGCAGCCG GAGAGGAGGT GAACAGTTTT
    AACCTCGTTG CTGCTGTGCT CCAACAGGAA ATGTTCGCTT CCATGAATCG TGCGCTTGAC
    CGGTCCTATA GTGATAAGGA TTGGCAACTG CTCACGTCCG TCATGCGCTG TTACACCCAG
    ATCTTCCTCA CAGTCCAGGA GATGTCAGAG TCTCCCAACG AGGAAGACCA GGAGATTGCC
    GAAAACACCC TGAGCCGTCT CTTTTACGAG GAGACAACCC ACGACCTTAT CGCGAATATT
    GCTCGAACAT ACAAGGATCA AGGCTTCGAA TATCTTGATG CCGCGACCGA GCTTGTCCAC
    ACTTTCCTCC GCATTTTAGA AGGTTACTCT AAGCAAAATG TCGATTTGCA GGTGCGCTCA
    CGGAAGCGAG CTCGCCGAAA GAAGAAGGCT GCCAAAGCTG CTGCTGCTGT TGCTGCAGCT
    AGAGCCGCCG GTGAGGAAGC AGAAGACGTT GGCGTTCCCG AGGACAATGA TGCCGATGAT
    TCTGGGGACG ATGAGCAACA TGCAGAGCGG GTCACTCAGG AGCGCAAGTT CGAGTTTGGG
    AAATTCGCCA TTCGATTCGC TCCCCAGGGT GTTGTCGACA CATTTGTCGC CTTCACCAAA
    TTCTACCGGG ATTTGAACGA CGCACAGCTG AAGCGCGCTC ATCGCTACTT CTACCGCGTT
    GCCTTCAAAC TCGAGCTGAG CATCATGCTA TTCCGACTAG ACATCATCAA TCTCTTTTAC
    AACATGGTCC AAGGTCCCGA GCCCCTCGAC AAAAGCTCGC CGATGTTCAA GGAATGGGAG
    GAGCTCTCCA AGCAGATCAT TCGGAAATGT GTCAAAAAGC TTCAAGAGCG GCCTGCGTTG
    TTCACCGAGT TGCTCTTTTC CAAGATTGGC AGCACGACGC ACTTCCTTGA GCACGGCTAT
    GAGAAGCCTG TCACCACAAC TACGCCACGA CCTGGTGCCG AGCTGGAGTT CAAGCGAGCC
    ACAGAGCGCG ACGAGCAGAT TGGCATTGCG GTCAGCGTAC TCATCGACAA ACAGCAAGTC
    GAGCATCTGC AGTGGCTCAA GGATCAGCTG ACAAGTGCAA TGAGTGAGCG GCAAGCATGG
    GAGAACGTAG ACAAGGCCAT GGCAGCCACT ACCGAGGGGG CGGCTGATGG TGAAGCTGCC
    GACGAGAGAT CCAACAAATC AGCACCACCA CATATCACGA TCCGCCCTGA TACCGAGGCT
    CGCCGCACAG CCATGTTCAA GAACCCGCAC CTCCGTCTTC TCATGCGTCT AGTCGGTATG
    GAGCGTCTCA CGCCTACTCT CGACGAGACC CCCGACTCGA CCTGGATTCT CCCAGGCAGC
    CACACCGCCG AGGCGATCCA AGATACCATC GACCTCATCA ACAAGGCCGA GTTCTCCCCT
    CCCACCTTTG AGGACGGCGG CTCAGCCGAA GACCAGCTCC GGCGCAAGTC CGCCGCCGCT
    TCTCGCAGGA CGCGTGCCGC ATATGACGAT GATGAGGAAG AAATTCGCGG ATTCCTCGGT
    GACGATGACG ACGAAGACTT TCTCTTTGCG CCCGGCGGTC CTACCGCTCG CAAGCCGGAC
    GCTAGGCCGC AGAAGAAGCG TCAGCGCAAG CGCCGAAGAG AGGCTGGTAG TGGAGACGAG
    GAAGATGAGG GGGTGTCGGA TGAAGTGTTG GCTGCGCGTG CGAAGAAGAG GAGGGAGAAG
    GAGCTGGAGA AGATTAGGAA GATCAAGAGT GAGATGTACG TGCACGCAAG CGACGATGAA
    ACGGACGATG AGAGGGATCG GGAGTTCTTT GAGCGGGAGA GAAAGAGGCA GGAGACGAAG
    GATTCAAAGT TCGATAGCAT GCTTGGGGCG TTGGGGTTGT CAGTGTTGTC GCAGGTGAAT
    GGAGGGGAAA AGAGCGCGTG GGAGGCGGTG CTGGATGATG AGCCCGAGAG TGATGAGAGT
    GAGAATGAAG GGCGGAAGAA TGCGAAGCGG AGGAAGAAGC AGGTCGCTTC TGGTTCCGAA
    GAAGAGCAAG AGGAGGAGGA GGAAGAGGAG GAAGAGGAGG ACAGCGATGA AGAATTGCCA
    ACGAAACAGG CCAAGAGTAA AACCTCGAAA AGGAAGGCGG CGGTACCGTC AAAACGACCG
    GCAAGGAGGC CTGGGACAGC CAAGAAAAGG GCTGTCGTGG AGCTCAGCGA CAATGATGAA
    GATGAAGATG AGGAAGAGGA TGCCATGGAC GTAGATTCGG CAAACGAACG CACGACCAGA
    AATGAGGCAC CCTTACCCTC TAGCCCTGGT GAGGGGCTAG GGAGGAGAAT AGACAAGATG
    GCGATGGATG ATGGAGACGA GGATGAGGAT GATCAGCCGG TGGTCGCTGC TCGCCAACGG
    CCAAAAGCCA GGGGTGGATT CATCATTGAC AGCAGCGATG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B