NCU06484 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU06484
Entrez Gene ID	3873297
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_952031.2	XP_957124.1	hypothetical protein

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06380	XP_003717194.1
Neurospora crassa	NCU06484	XP_957124.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU06484 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU06484 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf04186	XM_952031.2 Latest version!	Neurospora crassa OR74A hypothetical protein (NCU06484), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU06484 ( XM_952031.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_957124.1 Neurospora crassa OR74A hypothetical protein (NCU06484), mRNA.

Price & Availability↑

CloneID	ONf04186
Clone ID Related Accession (Same CDS sequence)	XM_952031.2
Accession Version	XM_952031.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_952031.1.

ATGTCTAAGC GTAAAAAGGA CTTTCTCACG GATTTCGACC CTAACAAATC CGATCCCGAA 
GACGAAAACT TCGACCCCAT TGCCGAAAAG CAACGAACCC CGCGAAGTGC AAGGAAGCAA 
GCAAACACCC GCCGCCCAAA GAACCGAGGC GCTGCTGGCG GCGCGCGCAG GCGGAACAGC 
CGATACAAAG GATCCGACAT CGAAGACGAC GAAGATATCA GCGACAGCGA ACAAGACATC 
TCTTTCGGTG AAGAGGAAGA CGAGGAAGAA GAAGAAGACT CGGACGCTCC TGTCAACGCT 
ACGGGCAGGC GGATGCGCCA GGCTGCCGCA CGACACCAAT CGTACAGGGA AAGCAGTGAA 
GACGAAGACG GGATCCAGTC GTCTGACCAA GACAAAGACG TCGTCCAGGA GCCCGACGAA 
GAACATAGAG TGCCTTCGAT AGAGTCTCCT GAACAGAGTC CCAGCAAAAA GAAGTCTCGC 
ATCGTTGTGC TGAGTGTCAA AAAAGGCGCC AAGCCAAGCT CACAACGACC AAGACGTGGC 
GCTGCTGCCA AAGAACCAGC AACCATGGTT CCCCCGACCC GCCGCTCTAC GAGAGCCCGC 
ACGGAAGAGA CGGACGAGCC ATTGCTCGAG CTGTCCAACT CGGGAAGACA TACCCGCGTC 
ACCCGCGCAT CAACAAGAAG TAAAAGCCCG GAAGCCTTGA CCCACGCCAC CAGAGCTACC 
CGTGGTTCCA AGGGTGTTAA GAAACAACAA CCTCCACCCA TTCCCGAAGC CTCGCATGAG 
GAAGATGACG ACGAATTCAA GGTCGAGACG GATATACCCA ATGCTGACGA TGTGGACGAG 
CTGGCCGGTC AGGCGAACAT GGTCGATGCG CCCGCTGACC ATCCAGACGA ACACCAAGAG 
GGAAGGGCTG AACAGGTGGC CGAGGAAGGA GCTGCCCAAC AGGTTAGCGA AGAACAACCT 
GCACAGACCG AGAGCCAGCC AGCAGCAGAA GACGCAGACG GTGACGACGA AGACGATCGT 
CCGATTACTA GGCGGACTCG TGGGGCTAGA GCCGTCACAT CTACCACAGC TGCCTCGACC 
ACGACCACTG TCACAGCAGT TGAACCAGAG GGCGAAGGAC TCAGGAGCCG CCTCAGGCGT 
GGGTCTCGCT TGAAGAAGAG GTCGCTCGCT GAACCTAGTA GTGACTTTGA GCCAGGCGAG 
GAGTCGGGCG ATAACGAATG CTATGCCTCG GAGGCTTCAG TTAAAAAGAG TGGACGTAAA 
GGTTCCAGTG CAGATGAGTC TGCCGCTACA CCCGTTCGCG GACGTTCCAC CCGTGCTCGC 
GCTGCCAAAA GGCCTCGCCG CAACCAAGAT TCTGGTGATG AGGAAGTTGA GCTGGATAGG 
GAGGAGCTTG CTGAAGAACT CGAGGAGCTT CGCGAGAGCT CACGCTCGCG GCCGCGAAGA 
GCTGCCACTC GCCGTCGCCG CTCACCTTCT ATTCAGTACG AGGAACCCGC GGGCAAGAGA 
CGTCGCACTA CCCAGCGTGT TGATTACACT ATCCCGGCGA TCGACTTGGC CGCCCTCGAG 
GCCGAGGAAG ATGAAGAGCC TGTGGCAACG CCAGCAAGAG GCCGCAAGAG GAACGGAGGA 
AATGCAGGAT GGGACCGTCC TTTAAACACC ACCTATGGTC CCTTCGGTGG CGCTGGAATT 
GGCGCTCTGC TTGGAGGCCC TTGGGGCACC GGTGCTACGG GCGGCGTTGA TTCCGACAGC 
AGCGACGACG AGATGGTGCA ACGAACCGGT CCTGGCCAGA ACCCTATTGG CATGACGCCC 
ACTTCTGCCG CTCCTCCGGT TGGCTTATCC AATCCTGCGG CTCAGACCCA TAATGTGGAC 
GGCCTGGGTG GTATTGGAGG CGCCACACCT AACGTCGGAA AGGTGAAGAA TCTCAAGGCG 
TTTGCGGATG CGGATCCGCT TGGCGTCGAT ACGAGTGTTG ACTTTAGCAA AGTCGGAGGC 
TTGCAAGGCC ACATCGACCA ACTCAAGGAG ATGGTTCAGC TTCCCCTTTT GTATCCCGAA 
CTTTTCACCA GATTTCATGT TACACCACCA CGAGGTGTTC TTTTCCACGG TCCGCCCGGT 
ACCGGTAAAA CTCTCCTCGC CAGAGCCTTG GCCAACTCGG TCGGCTCCGG AGGCCGCAAG 
ATCAGCTTCT ACATGCGCAA AGGAGCTGAC GCTCTCAGCA AGTGGGTTGG TGAGGCAGAG 
AAGCAGCTGC GGTTGCTCTT CGAGGAAGCT CGGAGGACGC AACCCAGTAT CATCTTCTTC 
GATGAGATCG ATGGTCTTGC CCCTGTGCGA TCCAGCAAGC AGGAGCAGAT CCATGCAAGC 
ATTGTCTCTA CCCTGCTGGC GTTGATGGAT GGCATGGACG GTCGTGGACA AGTAATTGTC 
ATCGGTGCCA CTAACCGCCC TGACAACATC GACCCTGCTT TGCGTCGCCC CGGCCGTTTC 
GATCGAGAAT TCTACTTCCC CCTTCCCGAC ATCGAAGGAA GGCGATCGAT TCTCGAGATT 
CACACTAAGG ACTGGGGTTT GTCGAACGAG TTCAAAGACC AGTTGGCCGA ATTCACCAAG 
GGATATGGCG GTGCCGATCT CCGTGCCCTT TGCACCGAGG CGGCTCTGAA TGCCATTCAG 
AGAACGTATC CCCAGATCTA TACATCCAAG GAAAAGCTTG TGGTCAATCC TCAGAAGATC 
AGCATTCAGG CGTCAGATTT CATGCACTCT ATCAAGAAGA TGGTGCCCTC GTCTGAGCGT 
TCGGCGTCAT CCAGCGCCAT GACCATCCCC AAAATGGTCA AGCCACTTCT GGAGAAGCAA 
TTTGAGGCTC TCGTTGCGCA GTTGGATAAG ATTCTGCCTC GATCAAAGAA GACCACCGCG 
CTGGAGGAGG CCATGTTTGA GCCGTATAAG GATCTCGATG GCGGGTTCGA GAGGGAGCAG 
ATGAGTCAGG ACTTCCAGCG GTCGCGTGTC TATCGCCCTC GCTTGCTCCT CTGTGGAGGG 
GCGGGCATGG GACACGGTTA CCTGTCGAAG GCTGTTTTGC ATTACTTGGA AAGTGTTCAT 
GTTCAGGATT TTGGTCTGCC TGTTCTACTG AATGATTCGA GGCCCCCTGA ACAAGTCATT 
GTGAGCCTCT TCACCGAGGT CAGACGTCAC AAGCCCAGTG TAATCTTTAT TCCCAATGTG 
GACGCTTGGT GGACAACCCT TGGCTATGCT ACCCTGACGA CATTCACGAC GATGCTTCGA 
CAGATATCGC CGACCGATCC TGTTCTTGTA TTCGGCACAG CGGAGTCGAT GCCTGACCTG 
CTCCCCCCCG AGATGTTAAC CGAACTGTTC GGGTTCTCTA AGAAGAACCG TGCCGCGATC 
TGGCGTCCGG ACCGTGAACA ACGTGTCGCC TTCTTTCTCC CTATCATCCA GAACCTGTGG 
AAGGCTCCCG AGGAATTTCC GGATCTCTCC AGCCGCAAGA AGCGGGTGCT TGAAATTTTG 
CCGGTTGCTC CGCCGCCGCC ACCCAGGGTT CCTACCAAGG AGGAGATTAA AGCTCAGCGC 
CAGATTGATT TCCACCACTT GAACTTACTG AAGGCGAGAC TGCAACCGAT CATGGATCAG 
ATTCAGCGCA GGTATCGCAA GTTCAGACAA CCTGTCATTC CTCTCAACAC GATCAGCTAT 
CTCTTCCAGG AGTCTGATCC CAACTTTGTC CGTCCGGATG TGGGTGAACA GGAGCAGCGG 
CCTTTCGTCA TTGCCAAGGA CGATAAGGGT GTTGACGGCA TCCTTGAAAC CAAGACGGGC 
AAGTTTTACT ACAACCTCGA CTCTACCACA ATTGAGGAGC GCTTGGCCAA TGGCTATTAC 
GCCCGCCCGA TGGACTTTTA TGAAGATATT AAGCGTTTAT TCCTCGATGT CAAGACTATC 
GGCGATAAGG ACTATCTCCC AAAGGCAAGC GAGATGGTTA CGAACGTTGA AATTGATGTC 
TACGATATAA ACCTTTCTTT CAAAACACAA GGCATCAACT TTGAGGATAT TTACCAAAGG 
CAGCTCGAAA GAACAAGGAA GGCCGAAGAG CGTCACCGCA AGAAGGCGGC TTTCCAGCCG 
ATGGTCGACA AGATCCAGTC TGACCTTGCC GAGACCCAAG ACGATAGCGA GTCTCAAGGC 
CCTGTTGGTA TTGGTTTTCC AATGAACAAG CCCGCCCACA CGACAGCGGC CAGATTTCAG 
GTCATCACAA GCCCCAAGTC GACCGGAAAC CCGAACTCTT CTGGATCGCA CCCGCTTACC 
AATGGCACAT CGCACCCTTC CAACCAAGGC GAGACCGGCC AGACGGTGGA AGAAGAAAGC 
ACTCTGCTCT TCTCTCAGGG AGTTGAAATC AGCTCGCCGC TTAGATATCG GCAAGCGCAA 
CGACAGCTTC AACAGAACAG AGTCATCACA GAGACAAATA GAGAAACCAT CGGTACATTC 
TCGCAGACAT CAGCACTTAC ATCTGTGCCT CCAGGAGTCT CCCCGGCCGC TATCCAGAAT 
GATGCTTCGA GTACCAGGAC TTCTGACCCA AGCTCAGGCC GTGGAGAGTG GAACACGCAA 
CAGACGAATG GCGTGCAGAG CATCTCTCGG GGAACATCCC AGTTGCTTGA GAGCCAGGAG 
ACGACTGTTA ACCCTCCTGC TGCGGCGTTC CCTGTGGAAA GCAACAGTCA GAGCCAAAGC 
CAGAGCAATT CTTCTAGTAG CGGCCCGTGG CCTCATTCAC AAATGGCTGG CATTGCGCAG 
GGCATCCTGC TGCCGGCCGT GCCTGAGGAG GCTGAGGCCC ACGATACTAA CGAAGTTCCC 
GCCGGTGGTC TCCGTTCGTC ATCCTCTAAG GACCAGCCGC AGCAGGGTAG CAGTAATAAT 
GACAGTCAAA ACAGCAAACC CAGCAATGAT GACAGTCAAA ACAGCAAACC CAGCAATGAC 
ACTCCTGATA GCCAGCGGAG TCTGTTAGAG GCCAGACGTC TTAGGGACCT TGCGATTGCG 
GACTGTATGG AGAGGGTTCT TCAAGGGCCC GTCCGCCCTG GCAGCACCGG CGGTAAGAAG 
ACGGATAGCT CCCCGTACAG CAACAACAGC CCGGCCCTTG GCCGGAACAA TGTCTATCTG 
CCCTTCGGCC GTCGCGACAC TTCGGTCTCA TCTCAGCAAC AGCCTATCCT TAACGACGGT 
AAGGTCTGCG AGTTCTGGTC CACGCTCGTG GACCGCACGG CCGGCTGCAA CATTGAGCAG 
ATGGAGCAGA TCCATAGAGA GCTGATGGAT GCCATCTGGC AGTACCGCCA CGAGTGGAAT 
CGTATGCGTG TCCTCTCCAC GCTCGCCGAC GTCTTTGACG ATACAGTTAC CGACATTGAG 
CTCGTGCAGG GCATCTTGAT TGATGCTCAG GGCAGAGAGC AGAGGGAGTA TGAAAGGAAG 
GAGCTTGAGA AGAGGGAGGC CGAGGCCGCC AAGAGTTCGT CGTCGACTTC TGCTCAGTCG 
CGGGCCGCGA CTCAGACTCA AGCTGCTGTT GCCGCTGTCC CCGTCGACGC TGCCGCTTCC 
TCCACCGGTG TCGCTGCCGG TTCAGTTGCT GCTCCTGGTC CAGCTGCTGT TGCTGCTGCT 
CGAGGTGTTC CCACCCCTGC TCCCACTGTT GTTTCAGCTT CAGCCCCTAG CGCGGTCTCC 
GCCAACGTTG CTGCTGTTGC TGGAAATGGC TCAGAAAGCC AGGGTACGAG CGGTAGCGGT 
ACTGGTAGCA CCAATAACAG CCAGAAGAGC CACAGCAGCC AGAAGAGCAA GAGCAACAAT 
AGTGGAAAGA GTAGTAGCCA GAAGACACCT ACTAGCAATA GTACGAGCCA GAAGCGCAAC 
AAGGCGGCAG CGGAGGAGGA GAGGTTGCCT TATATCTGGC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_957124.1
CDS	1100..6967
Translation	MSKRKKDFLTDFDPNKSDPEDENFDPIAEKQRTPRSARKQANTRRPKNRGAAGGARRRNSRYKGSDIEDDEDISDSEQDISFGEEEDEEEEEDSDAPVNATGRRMRQAAARHQSYRESSEDEDGIQSSDQDKDVVQEPDEEHRVPSIESPEQSPSKKKSRIVVLSVKKGAKPSSQRPRRGAAAKEPATMVPPTRRSTRARTEETDEPLLELSNSGRHTRVTRASTRSKSPEALTHATRATRGSKGVKKQQPPPIPEASHEEDDDEFKVETDIPNADDVDELAGQANMVDAPADHPDEHQEGRAEQVAEEGAAQQVSEEQPAQTESQPAAEDADGDDEDDRPITRRTRGARAVTSTTAASTTTTVTAVEPEGEGLRSRLRRGSRLKKRSLAEPSSDFEPGEESGDNECYASEASVKKSGRKGSSADESAATPVRGRSTRARAAKRPRRNQDSGDEEVELDREELAEELEELRESSRSRPRRAATRRRRSPSIQYEEPAGKRRRTTQRVDYTIPAIDLAALEAEEDEEPVATPARGRKRNGGNAGWDRPLNTTYGPFGGAGIGALLGGPWGTGATGGVDSDSSDDEMVQRTGPGQNPIGMTPTSAAPPVGLSNPAAQTHNVDGLGGIGGATPNVGKVKNLKAFADADPLGVDTSVDFSKVGGLQGHIDQLKEMVQLPLLYPELFTRFHVTPPRGVLFHGPPGTGKTLLARALANSVGSGGRKISFYMRKGADALSKWVGEAEKQLRLLFEEARRTQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLLALMDGMDGRGQVIVIGATNRPDNIDPALRRPGRFDREFYFPLPDIEGRRSILEIHTKDWGLSNEFKDQLAEFTKGYGGADLRALCTEAALNAIQRTYPQIYTSKEKLVVNPQKISIQASDFMHSIKKMVPSSERSASSSAMTIPKMVKPLLEKQFEALVAQLDKILPRSKKTTALEEAMFEPYKDLDGGFEREQMSQDFQRSRVYRPRLLLCGGAGMGHGYLSKAVLHYLESVHVQDFGLPVLLNDSRPPEQVIVSLFTEVRRHKPSVIFIPNVDAWWTTLGYATLTTFTTMLRQISPTDPVLVFGTAESMPDLLPPEMLTELFGFSKKNRAAIWRPDREQRVAFFLPIIQNLWKAPEEFPDLSSRKKRVLEILPVAPPPPPRVPTKEEIKAQRQIDFHHLNLLKARLQPIMDQIQRRYRKFRQPVIPLNTISYLFQESDPNFVRPDVGEQEQRPFVIAKDDKGVDGILETKTGKFYYNLDSTTIEERLANGYYARPMDFYEDIKRLFLDVKTIGDKDYLPKASEMVTNVEIDVYDINLSFKTQGINFEDIYQRQLERTRKAEERHRKKAAFQPMVDKIQSDLAETQDDSESQGPVGIGFPMNKPAHTTAARFQVITSPKSTGNPNSSGSHPLTNGTSHPSNQGETGQTVEEESTLLFSQGVEISSPLRYRQAQRQLQQNRVITETNRETIGTFSQTSALTSVPPGVSPAAIQNDASSTRTSDPSSGRGEWNTQQTNGVQSISRGTSQLLESQETTVNPPAAAFPVESNSQSQSQSNSSSSGPWPHSQMAGIAQGILLPAVPEEAEAHDTNEVPAGGLRSSSSKDQPQQGSSNNDSQNSKPSNDDSQNSKPSNDTPDSQRSLLEARRLRDLAIADCMERVLQGPVRPGSTGGKKTDSSPYSNNSPALGRNNVYLPFGRRDTSVSSQQQPILNDGKVCEFWSTLVDRTAGCNIEQMEQIHRELMDAIWQYRHEWNRMRVLSTLADVFDDTVTDIELVQGILIDAQGREQREYERKELEKREAEAAKSSSSTSAQSRAATQTQAAVAAVPVDAAASSTGVAAGSVAAPGPAAVAAARGVPTPAPTVVSASAPSAVSANVAAVAGNGSESQGTSGSGTGSTNNSQKSHSSQKSKSNNSGKSSSQKTPTSNSTSQKRNKAAAEEERLPYIWLK

Target ORF information:

RefSeq Version	XM_952031.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A hypothetical protein (NCU06484), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_952031.2

ORF Insert Sequence:

ATGTCTAAGC GTAAAAAGGA CTTTCTCACG GATTTCGACC CTAACAAATC CGATCCCGAA 
GACGAAAACT TCGACCCCAT TGCCGAAAAG CAACGAACCC CGCGAAGTGC AAGGAAGCAA 
GCAAACACCC GCCGCCCAAA GAACCGAGGC GCTGCTGGCG GCGCGCGCAG GCGGAACAGC 
CGATACAAAG GATCCGACAT CGAAGACGAC GAAGATATCA GCGACAGCGA ACAAGACATC 
TCTTTCGGTG AAGAGGAAGA CGAGGAAGAA GAAGAAGACT CGGACGCTCC TGTCAACGCT 
ACGGGCAGGC GGATGCGCCA GGCTGCCGCA CGACACCAAT CGTACAGGGA AAGCAGTGAA 
GACGAAGACG GGATCCAGTC GTCTGACCAA GACAAAGACG TCGTCCAGGA GCCCGACGAA 
GAACATAGAG TGCCTTCGAT AGAGTCTCCT GAACAGAGTC CCAGCAAAAA GAAGTCTCGC 
ATCGTTGTGC TGAGTGTCAA AAAAGGCGCC AAGCCAAGCT CACAACGACC AAGACGTGGC 
GCTGCTGCCA AAGAACCAGC AACCATGGTT CCCCCGACCC GCCGCTCTAC GAGAGCCCGC 
ACGGAAGAGA CGGACGAGCC ATTGCTCGAG CTGTCCAACT CGGGAAGACA TACCCGCGTC 
ACCCGCGCAT CAACAAGAAG TAAAAGCCCG GAAGCCTTGA CCCACGCCAC CAGAGCTACC 
CGTGGTTCCA AGGGTGTTAA GAAACAACAA CCTCCACCCA TTCCCGAAGC CTCGCATGAG 
GAAGATGACG ACGAATTCAA GGTCGAGACG GATATACCCA ATGCTGACGA TGTGGACGAG 
CTGGCCGGTC AGGCGAACAT GGTCGATGCG CCCGCTGACC ATCCAGACGA ACACCAAGAG 
GGAAGGGCTG AACAGGTGGC CGAGGAAGGA GCTGCCCAAC AGGTTAGCGA AGAACAACCT 
GCACAGACCG AGAGCCAGCC AGCAGCAGAA GACGCAGACG GTGACGACGA AGACGATCGT 
CCGATTACTA GGCGGACTCG TGGGGCTAGA GCCGTCACAT CTACCACAGC TGCCTCGACC 
ACGACCACTG TCACAGCAGT TGAACCAGAG GGCGAAGGAC TCAGGAGCCG CCTCAGGCGT 
GGGTCTCGCT TGAAGAAGAG GTCGCTCGCT GAACCTAGTA GTGACTTTGA GCCAGGCGAG 
GAGTCGGGCG ATAACGAATG CTATGCCTCG GAGGCTTCAG TTAAAAAGAG TGGACGTAAA 
GGTTCCAGTG CAGATGAGTC TGCCGCTACA CCCGTTCGCG GACGTTCCAC CCGTGCTCGC 
GCTGCCAAAA GGCCTCGCCG CAACCAAGAT TCTGGTGATG AGGAAGTTGA GCTGGATAGG 
GAGGAGCTTG CTGAAGAACT CGAGGAGCTT CGCGAGAGCT CACGCTCGCG GCCGCGAAGA 
GCTGCCACTC GCCGTCGCCG CTCACCTTCT ATTCAGTACG AGGAACCCGC GGGCAAGAGA 
CGTCGCACTA CCCAGCGTGT TGATTACACT ATCCCGGCGA TCGACTTGGC CGCCCTCGAG 
GCCGAGGAAG ATGAAGAGCC TGTGGCAACG CCAGCAAGAG GCCGCAAGAG GAACGGAGGA 
AATGCAGGAT GGGACCGTCC TTTAAACACC ACCTATGGTC CCTTCGGTGG CGCTGGAATT 
GGCGCTCTGC TTGGAGGCCC TTGGGGCACC GGTGCTACGG GCGGCGTTGA TTCCGACAGC 
AGCGACGACG AGATGGTGCA ACGAACCGGT CCTGGCCAGA ACCCTATTGG CATGACGCCC 
ACTTCTGCCG CTCCTCCGGT TGGCTTATCC AATCCTGCGG CTCAGACCCA TAATGTGGAC 
GGCCTGGGTG GTATTGGAGG CGCCACACCT AACGTCGGAA AGGTGAAGAA TCTCAAGGCG 
TTTGCGGATG CGGATCCGCT TGGCGTCGAT ACGAGTGTTG ACTTTAGCAA AGTCGGAGGC 
TTGCAAGGCC ACATCGACCA ACTCAAGGAG ATGGTTCAGC TTCCCCTTTT GTATCCCGAA 
CTTTTCACCA GATTTCATGT TACACCACCA CGAGGTGTTC TTTTCCACGG TCCGCCCGGT 
ACCGGTAAAA CTCTCCTCGC CAGAGCCTTG GCCAACTCGG TCGGCTCCGG AGGCCGCAAG 
ATCAGCTTCT ACATGCGCAA AGGAGCTGAC GCTCTCAGCA AGTGGGTTGG TGAGGCAGAG 
AAGCAGCTGC GGTTGCTCTT CGAGGAAGCT CGGAGGACGC AACCCAGTAT CATCTTCTTC 
GATGAGATCG ATGGTCTTGC CCCTGTGCGA TCCAGCAAGC AGGAGCAGAT CCATGCAAGC 
ATTGTCTCTA CCCTGCTGGC GTTGATGGAT GGCATGGACG GTCGTGGACA AGTAATTGTC 
ATCGGTGCCA CTAACCGCCC TGACAACATC GACCCTGCTT TGCGTCGCCC CGGCCGTTTC 
GATCGAGAAT TCTACTTCCC CCTTCCCGAC ATCGAAGGAA GGCGATCGAT TCTCGAGATT 
CACACTAAGG ACTGGGGTTT GTCGAACGAG TTCAAAGACC AGTTGGCCGA ATTCACCAAG 
GGATATGGCG GTGCCGATCT CCGTGCCCTT TGCACCGAGG CGGCTCTGAA TGCCATTCAG 
AGAACGTATC CCCAGATCTA TACATCCAAG GAAAAGCTTG TGGTCAATCC TCAGAAGATC 
AGCATTCAGG CGTCAGATTT CATGCACTCT ATCAAGAAGA TGGTGCCCTC GTCTGAGCGT 
TCGGCGTCAT CCAGCGCCAT GACCATCCCC AAAATGGTCA AGCCACTTCT GGAGAAGCAA 
TTTGAGGCTC TCGTTGCGCA GTTGGATAAG ATTCTGCCTC GATCAAAGAA GACCACCGCG 
CTGGAGGAGG CCATGTTTGA GCCGTATAAG GATCTCGATG GCGGGTTCGA GAGGGAGCAG 
ATGAGTCAGG ACTTCCAGCG GTCGCGTGTC TATCGCCCTC GCTTGCTCCT CTGTGGAGGG 
GCGGGCATGG GACACGGTTA CCTGTCGAAG GCTGTTTTGC ATTACTTGGA AAGTGTTCAT 
GTTCAGGATT TTGGTCTGCC TGTTCTACTG AATGATTCGA GGCCCCCTGA ACAAGTCATT 
GTGAGCCTCT TCACCGAGGT CAGACGTCAC AAGCCCAGTG TAATCTTTAT TCCCAATGTG 
GACGCTTGGT GGACAACCCT TGGCTATGCT ACCCTGACGA CATTCACGAC GATGCTTCGA 
CAGATATCGC CGACCGATCC TGTTCTTGTA TTCGGCACAG CGGAGTCGAT GCCTGACCTG 
CTCCCCCCCG AGATGTTAAC CGAACTGTTC GGGTTCTCTA AGAAGAACCG TGCCGCGATC 
TGGCGTCCGG ACCGTGAACA ACGTGTCGCC TTCTTTCTCC CTATCATCCA GAACCTGTGG 
AAGGCTCCCG AGGAATTTCC GGATCTCTCC AGCCGCAAGA AGCGGGTGCT TGAAATTTTG 
CCGGTTGCTC CGCCGCCGCC ACCCAGGGTT CCTACCAAGG AGGAGATTAA AGCTCAGCGC 
CAGATTGATT TCCACCACTT GAACTTACTG AAGGCGAGAC TGCAACCGAT CATGGATCAG 
ATTCAGCGCA GGTATCGCAA GTTCAGACAA CCTGTCATTC CTCTCAACAC GATCAGCTAT 
CTCTTCCAGG AGTCTGATCC CAACTTTGTC CGTCCGGATG TGGGTGAACA GGAGCAGCGG 
CCTTTCGTCA TTGCCAAGGA CGATAAGGGT GTTGACGGCA TCCTTGAAAC CAAGACGGGC 
AAGTTTTACT ACAACCTCGA CTCTACCACA ATTGAGGAGC GCTTGGCCAA TGGCTATTAC 
GCCCGCCCGA TGGACTTTTA TGAAGATATT AAGCGTTTAT TCCTCGATGT CAAGACTATC 
GGCGATAAGG ACTATCTCCC AAAGGCAAGC GAGATGGTTA CGAACGTTGA AATTGATGTC 
TACGATATAA ACCTTTCTTT CAAAACACAA GGCATCAACT TTGAGGATAT TTACCAAAGG 
CAGCTCGAAA GAACAAGGAA GGCCGAAGAG CGTCACCGCA AGAAGGCGGC TTTCCAGCCG 
ATGGTCGACA AGATCCAGTC TGACCTTGCC GAGACCCAAG ACGATAGCGA GTCTCAAGGC 
CCTGTTGGTA TTGGTTTTCC AATGAACAAG CCCGCCCACA CGACAGCGGC CAGATTTCAG 
GTCATCACAA GCCCCAAGTC GACCGGAAAC CCGAACTCTT CTGGATCGCA CCCGCTTACC 
AATGGCACAT CGCACCCTTC CAACCAAGGC GAGACCGGCC AGACGGTGGA AGAAGAAAGC 
ACTCTGCTCT TCTCTCAGGG AGTTGAAATC AGCTCGCCGC TTAGATATCG GCAAGCGCAA 
CGACAGCTTC AACAGAACAG AGTCATCACA GAGACAAATA GAGAAACCAT CGGTACATTC 
TCGCAGACAT CAGCACTTAC ATCTGTGCCT CCAGGAGTCT CCCCGGCCGC TATCCAGAAT 
GATGCTTCGA GTACCAGGAC TTCTGACCCA AGCTCAGGCC GTGGAGAGTG GAACACGCAA 
CAGACGAATG GCGTGCAGAG CATCTCTCGG GGAACATCCC AGTTGCTTGA GAGCCAGGAG 
ACGACTGTTA ACCCTCCTGC TGCGGCGTTC CCTGTGGAAA GCAACAGTCA GAGCCAAAGC 
CAGAGCAATT CTTCTAGTAG CGGCCCGTGG CCTCATTCAC AAATGGCTGG CATTGCGCAG 
GGCATCCTGC TGCCGGCCGT GCCTGAGGAG GCTGAGGCCC ACGATACTAA CGAAGTTCCC 
GCCGGTGGTC TCCGTTCGTC ATCCTCTAAG GACCAGCCGC AGCAGGGTAG CAGTAATAAT 
GACAGTCAAA ACAGCAAACC CAGCAATGAT GACAGTCAAA ACAGCAAACC CAGCAATGAC 
ACTCCTGATA GCCAGCGGAG TCTGTTAGAG GCCAGACGTC TTAGGGACCT TGCGATTGCG 
GACTGTATGG AGAGGGTTCT TCAAGGGCCC GTCCGCCCTG GCAGCACCGG CGGTAAGAAG 
ACGGATAGCT CCCCGTACAG CAACAACAGC CCGGCCCTTG GCCGGAACAA TGTCTATCTG 
CCCTTCGGCC GTCGCGACAC TTCGGTCTCA TCTCAGCAAC AGCCTATCCT TAACGACGGT 
AAGGTCTGCG AGTTCTGGTC CACGCTCGTG GACCGCACGG CCGGCTGCAA CATTGAGCAG 
ATGGAGCAGA TCCATAGAGA GCTGATGGAT GCCATCTGGC AGTACCGCCA CGAGTGGAAT 
CGTATGCGTG TCCTCTCCAC GCTCGCCGAC GTCTTTGACG ATACAGTTAC CGACATTGAG 
CTCGTGCAGG GCATCTTGAT TGATGCTCAG GGCAGAGAGC AGAGGGAGTA TGAAAGGAAG 
GAGCTTGAGA AGAGGGAGGC CGAGGCCGCC AAGAGTTCGT CGTCGACTTC TGCTCAGTCG 
CGGGCCGCGA CTCAGACTCA AGCTGCTGTT GCCGCTGTCC CCGTCGACGC TGCCGCTTCC 
TCCACCGGTG TCGCTGCCGG TTCAGTTGCT GCTCCTGGTC CAGCTGCTGT TGCTGCTGCT 
CGAGGTGTTC CCACCCCTGC TCCCACTGTT GTTTCAGCTT CAGCCCCTAG CGCGGTCTCC 
GCCAACGTTG CTGCTGTTGC TGGAAATGGC TCAGAAAGCC AGGGTACGAG CGGTAGCGGT 
ACTGGTAGCA CCAATAACAG CCAGAAGAGC CACAGCAGCC AGAAGAGCAA GAGCAACAAT 
AGTGGAAAGA GTAGTAGCCA GAAGACACCT ACTAGCAATA GTACGAGCCA GAAGCGCAAC 
AAGGCGGCAG CGGAGGAGGA GAGGTTGCCT TATATCTGGC TGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU06484 cDNA ORF clone, Neurospora crassa OR74A

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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