ECE1 cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECE1
Entrez Gene ID	104410063
Full Name	endothelin converting enzyme 1
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010020119.1	XP_010018421.1	LOW QUALITY PROTEIN: endothelin-converting enzyme 1

Pathways from BioSystems
Gene Ontology

The following ECE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe116968	XM_010020119.1 Latest version!	Nestor notabilis endothelin converting enzyme 1 (ECE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECE1 ( XM_010020119.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010018421.1 Nestor notabilis endothelin converting enzyme 1 (ECE1), mRNA.

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CloneID	ONe116968
Clone ID Related Accession (Same CDS sequence)	XM_010020119.1
Accession Version	XM_010020119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	LOW QUALITY PROTEIN: endothelin-converting enzyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009939945.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 3 indels ##RefSeq-Attributes-END##

ATGTCGACCT ACAAGCGAGC AACGCTGGAT GACGAAGACC CCCTGGACTC CATTGGTGAT 
GGTGATGGGT ACCCCAATGG CTTCCAGGTG AACTTCCGCA GCTCAAGGAG CGCCCCGGGG 
TGCTGGGCTG AGCGGACACG CGCCGAGAAG CAGCTCGTGG TGCTGGGGGG GGCTGGGGGG 
CTGGGGGCCG TGCTGGTGGC GTGTCTGCTG GGTCTCATCT TCCAGTACAG AGCCCGGGTG 
CCGGCTGTGT GCCTGTCGGA AGCCTGCATC TCCGTCACCA GCTCCATCCT CAGCTCGCTG 
GACCGGGCGG TGAATCCCTG TGAGGACTTC TTCAGCTACG CCTGCGGGGG CTGGATCAAG 
GCCAACCCCC TCCCTGATGG CCACTCACGC TGGGGCACGT TCAACAACCT CTGGGAGCAC 
AACCAGGCCA TCATGAAGCA TCTGCTGGAA AACACCACGG CCAACGTGTC GAGCGAGGCA 
GAGCGCAAAG CGCAGCGTTA CTACCAAGCT TGCATGAATG AGAGCAAGAT CGAGGAGCTG 
CGTGCCACCC CGCTTGTGGA GCTCATCCAA AAGCTGGGTG GCTGGAACAT CACGGGTCCA 
TGGGCCGGTG ACAACTTCAA CNNTCGGGAG GTGACGGCGC ATTACCGCAC CTCCCCCTTC 
TTCTCCGTCT ACGTCAGTGC CGACTCCAAA AACTCCAACA GCAACGTGAT CCAGGTGGAT 
CAGTCGGGGT TGGGCCTGCC CTCACGGGAT TACTACCTCA ACAAGACAGA GAATGAGAAG 
GTGCTCGCCG GGTACCTGAA CTACATGGTG CAGTTGGGGA TGTTCTTGGG AGGCACTGAT 
GAGGAGTCGA CTCGGCAGCA GATGCAGCAG ATCTTGGACT TTGAGACGGC GTTGGCCAAC 
ATCACCATCC CACAGGAGAA GCACCGGGAT GAGGAGGTCA TCTACCATAA AATGACGGCT 
GGAGACCTAA AGGAGCTGGC ACCAGCCGTG GACTGGATGC CCTTCCTCAG CACGGTGTTC 
TACCCTGTGG AGCTCAATGA ATCTGAGCCC GTCGTGGTCT ATGCCAAGGA GTACCTGGAG 
CAGGTCTCTG ACCTCATCCT GGCCACAGAT AAATGTCTCC TCAACAACTA CATGATCTGG 
AACCTGGTGC GGAAAACCAG CCCCTTCCTT GACCAGCGCT TCCAGGACGC TGAGGAAAAA 
TTCATGGAAG TGATGTATGG GACGAAGAAG ACCTGCCTCC CACGCTGGAA GTTCTGCATC 
AGTGACACTG ACAACAACCT GGGCTTCGCC CTGGGGGCCA TGTTTGTCAA GGCCACCTTC 
GCCGAGGACA GCAAGCAAGT GGCAGAGGAA ATGATTGCAG AGATTAAAAC AGCCTTTGAG 
GAAAGCCTGG AGACCCTGCA GTGGATGGAT GAAGAGACAA GGAAATCAGC CAAAGAGAAG 
GCAGATGCCA TCTACAACAT GATTGGCTAC CCCAAGTTCA TCATGGACCC CAAGGAGCTG 
GATAAAGTCT TTAATGACTA CGAGGCCGTG TCTGACCTCT ACTTTGAGAA CGTCATGCAG 
TTTTACAACT TCTCGGCCAG AGTCACCGCT GACCAGCTCC GGAAACCACC AAACCGGGAC 
CAGTGGAGCA TGACACCCCC GACGGTCAAT GCGTACTACT CTCCCACCAA GAATGAGATT 
GTCTTTCCTG CTGGCATCCT CCAGGCCCCT TTTTACACAC GTGCATCTCC CAAGTCACTG 
AATTTTGGTG GGATCGGCGT GGTCGTGGGC CATGAGTTGA CACATGCCTT TGATGATCAA 
GGCCGGGAAT ATGACAAGGA TGGCAACCTG CGTCCCTGGT GGAAGAATTC CTCTGTGGAG 
GCCTTCAAGC GTCAGACAGC GTGTATGGTG GAGCAGTATG GCAACTACAC CGTCAACGGA 
GAGGCTGTCA ATGGCAAGCA CACCCTCGGG GAGAACATCG CCGACAACGG GGGCCTCAAG 
GCGGCCTACC GGGCATATCA AAACTGGCTG AAAAAGAATG GGGATGAGGA AACCCTCCCA 
ACTCTCGGCC TCACCAACCA CCAGCTCTTC TTTGTTGGCT TCGCACAGGT GTGGTGCTCG 
GTTCGCACGC CGGAGAGCTC GCACGAGGGG CTCATCACAG ACCCCCACAG CCCCTCGCGA 
TTCCGGGTCA TCGGCACCGT CTCCAACTCC CAGGAATTCG CGGAGCATTT CGGCTGCCCC 
NNCTCCCCCG TGACCCCCCC CAGGAAATGT GAGGTCTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010018421.1
CDS	1..2262
Translation	MSTYKRATLDDEDPLDSIGDGDGYPNGFQVNFRSSRSAPGCWAERTRAEKQLVVLGGAGGLGAVLVACLLGLIFQYRARVPAVCLSEACISVTSSILSSLDRAVNPCEDFFSYACGGWIKANPLPDGHSRWGTFNNLWEHNQAIMKHLLENTTANVSSEAERKAQRYYQACMNESKIEELRATPLVELIQKLGGWNITGPWAGDNFNnullREVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLAGYLNYMVQLGMFLGGTDEESTRQQMQQILDFETALANITIPQEKHRDEEVIYHKMTAGDLKELAPAVDWMPFLSTVFYPVELNESEPVVVYAKEYLEQVSDLILATDKCLLNNYMIWNLVRKTSPFLDQRFQDAEEKFMEVMYGTKKTCLPRWKFCISDTDNNLGFALGAMFVKATFAEDSKQVAEEMIAEIKTAFEESLETLQWMDEETRKSAKEKADAIYNMIGYPKFIMDPKELDKVFNDYEAVSDLYFENVMQFYNFSARVTADQLRKPPNRDQWSMTPPTVNAYYSPTKNEIVFPAGILQAPFYTRASPKSLNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTACMVEQYGNYTVNGEAVNGKHTLGENIADNGGLKAAYRAYQNWLKKNGDEETLPTLGLTNHQLFFVGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGTVSNSQEFAEHFGCPnullSPVTPPRKCEVW

Target ORF information:

RefSeq Version	XM_010020119.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis endothelin converting enzyme 1 (ECE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010020119.1

ORF Insert Sequence:

ATGTCGACCT ACAAGCGAGC AACGCTGGAT GACGAAGACC CCCTGGACTC CATTGGTGAT 
GGTGATGGGT ACCCCAATGG CTTCCAGGTG AACTTCCGCA GCTCAAGGAG CGCCCCGGGG 
TGCTGGGCTG AGCGGACACG CGCCGAGAAG CAGCTCGTGG TGCTGGGGGG GGCTGGGGGG 
CTGGGGGCCG TGCTGGTGGC GTGTCTGCTG GGTCTCATCT TCCAGTACAG AGCCCGGGTG 
CCGGCTGTGT GCCTGTCGGA AGCCTGCATC TCCGTCACCA GCTCCATCCT CAGCTCGCTG 
GACCGGGCGG TGAATCCCTG TGAGGACTTC TTCAGCTACG CCTGCGGGGG CTGGATCAAG 
GCCAACCCCC TCCCTGATGG CCACTCACGC TGGGGCACGT TCAACAACCT CTGGGAGCAC 
AACCAGGCCA TCATGAAGCA TCTGCTGGAA AACACCACGG CCAACGTGTC GAGCGAGGCA 
GAGCGCAAAG CGCAGCGTTA CTACCAAGCT TGCATGAATG AGAGCAAGAT CGAGGAGCTG 
CGTGCCACCC CGCTTGTGGA GCTCATCCAA AAGCTGGGTG GCTGGAACAT CACGGGTCCA 
TGGGCCGGTG ACAACTTCAA CNNTCGGGAG GTGACGGCGC ATTACCGCAC CTCCCCCTTC 
TTCTCCGTCT ACGTCAGTGC CGACTCCAAA AACTCCAACA GCAACGTGAT CCAGGTGGAT 
CAGTCGGGGT TGGGCCTGCC CTCACGGGAT TACTACCTCA ACAAGACAGA GAATGAGAAG 
GTGCTCGCCG GGTACCTGAA CTACATGGTG CAGTTGGGGA TGTTCTTGGG AGGCACTGAT 
GAGGAGTCGA CTCGGCAGCA GATGCAGCAG ATCTTGGACT TTGAGACGGC GTTGGCCAAC 
ATCACCATCC CACAGGAGAA GCACCGGGAT GAGGAGGTCA TCTACCATAA AATGACGGCT 
GGAGACCTAA AGGAGCTGGC ACCAGCCGTG GACTGGATGC CCTTCCTCAG CACGGTGTTC 
TACCCTGTGG AGCTCAATGA ATCTGAGCCC GTCGTGGTCT ATGCCAAGGA GTACCTGGAG 
CAGGTCTCTG ACCTCATCCT GGCCACAGAT AAATGTCTCC TCAACAACTA CATGATCTGG 
AACCTGGTGC GGAAAACCAG CCCCTTCCTT GACCAGCGCT TCCAGGACGC TGAGGAAAAA 
TTCATGGAAG TGATGTATGG GACGAAGAAG ACCTGCCTCC CACGCTGGAA GTTCTGCATC 
AGTGACACTG ACAACAACCT GGGCTTCGCC CTGGGGGCCA TGTTTGTCAA GGCCACCTTC 
GCCGAGGACA GCAAGCAAGT GGCAGAGGAA ATGATTGCAG AGATTAAAAC AGCCTTTGAG 
GAAAGCCTGG AGACCCTGCA GTGGATGGAT GAAGAGACAA GGAAATCAGC CAAAGAGAAG 
GCAGATGCCA TCTACAACAT GATTGGCTAC CCCAAGTTCA TCATGGACCC CAAGGAGCTG 
GATAAAGTCT TTAATGACTA CGAGGCCGTG TCTGACCTCT ACTTTGAGAA CGTCATGCAG 
TTTTACAACT TCTCGGCCAG AGTCACCGCT GACCAGCTCC GGAAACCACC AAACCGGGAC 
CAGTGGAGCA TGACACCCCC GACGGTCAAT GCGTACTACT CTCCCACCAA GAATGAGATT 
GTCTTTCCTG CTGGCATCCT CCAGGCCCCT TTTTACACAC GTGCATCTCC CAAGTCACTG 
AATTTTGGTG GGATCGGCGT GGTCGTGGGC CATGAGTTGA CACATGCCTT TGATGATCAA 
GGCCGGGAAT ATGACAAGGA TGGCAACCTG CGTCCCTGGT GGAAGAATTC CTCTGTGGAG 
GCCTTCAAGC GTCAGACAGC GTGTATGGTG GAGCAGTATG GCAACTACAC CGTCAACGGA 
GAGGCTGTCA ATGGCAAGCA CACCCTCGGG GAGAACATCG CCGACAACGG GGGCCTCAAG 
GCGGCCTACC GGGCATATCA AAACTGGCTG AAAAAGAATG GGGATGAGGA AACCCTCCCA 
ACTCTCGGCC TCACCAACCA CCAGCTCTTC TTTGTTGGCT TCGCACAGGT GTGGTGCTCG 
GTTCGCACGC CGGAGAGCTC GCACGAGGGG CTCATCACAG ACCCCCACAG CCCCTCGCGA 
TTCCGGGTCA TCGGCACCGT CTCCAACTCC CAGGAATTCG CGGAGCATTT CGGCTGCCCC 
NNCTCCCCCG TGACCCCCCC CAGGAAATGT GAGGTCTGGT GA