BLM cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	BLM
Entrez Gene ID	104399953
Full Name	Bloom syndrome RecQ like helicase
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010018827.1	XP_010017129.1	Bloom syndrome protein

Pathways from BioSystems
Gene Ontology

The following BLM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe116321	XM_010018827.1 Latest version!	Nestor notabilis Bloom syndrome, RecQ helicase-like (BLM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BLM ( XM_010018827.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010017129.1 Nestor notabilis Bloom syndrome, RecQ helicase-like (BLM), mRNA.

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CloneID	ONe116321
Clone ID Related Accession (Same CDS sequence)	XM_010018827.1
Accession Version	XM_010018827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	Bloom syndrome protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009937410.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA TCTGTAAGCT GGTAGAGGCC ATTCCACTGC AGGAGCTCAA GTCTCTGTCA 
TGTGCTAAAG ACCTTCTCCA GAAGCGAGAC CTCAGGAGAA AACTGCTCGC TAGTTCTGTT 
GGTTTGAACA AAAGTAGCTT CAAATGCAGC TTTCCCAGAG CCTGGCAGGT GCCTGTGGAG 
CGGGGCCCTC CCCCATGTCC TGGTGCTGCA CCATGTCCTG GCCTCAGCTG GAGCTTCTGT 
TCTGACAGCA ACTCGCCCAG AGCCACAAAC CCCCCATCCC TGCTGCTCGG TTCTGCTCAT 
TCAAGCAAGT TTTCTACGCC CAGAAATCAA ACCTTGGATA CCTCTTGTGC TTCAAAGCAA 
AGTGTTGAGG AGATCACCTG CCCAGGCACC ACGGGGCTGT CTTCCTCCAG GGTGAATGGT 
GCAAAGGGAG CTTCCCTCAG CCTCCATCCT GAGGTGACCC TCAACAGCTC CTGCTGTGAG 
CGCCCAGGGG TGAGGAACAG CGGAATCCAC CCTTTGCTGC AGAGGCCTCT TACAAGCACT 
GCTCTGAAGG GGCAGAGTAA GGCTCCCACT GACGTTCCCA CAGAAGACAC TTCAGGTATA 
AACGATGACT TTGACCTGGA CCCCTTCGAT ATTGATGATT TTGATGAGGG CTGGGAGGAC 
GTGGTGGATG TGTCGGCACC AAAGGGTCCA TCCACGCCGT CGTACCAGCC CATCAGGGAA 
GGGCCACCTG CCAAATCGCT GCTCTCAAAG ATAACGTCCA GGGCCAAGGG ATCTGCTGTT 
GTATCAAATC CTGCTGCTCC CAAGTCAAGC TCCTTGATGA CAACAAAGAA CTGCTCAGGT 
CCGGCAGTTG TTAACCCTGC ACTGGAGCGT TTCAGGGGTC TGAAATTTTC CCATTCTGAA 
GAAATGATGA CTATATTTCA CAAGAAGTTT GGGCTGCACT GCTTCAGGAC AAACCAGCTG 
GAGGCCATCA ACGCTGCTCT TCTGGGTGAA GACTGTTTCA TCTTAATGCC CACTGGTGGT 
GGTAAAAGCT TGTGCTACCA GTTACCAGCG TGTGTCTCTG CTGGAGTCAC TATTGTTATC 
TCTCCACTGA GGTCGCTGAT AATAGACCAG GTTCAGAAAC TGAAGACTTT AGATATTGCT 
GCAACATACC TGACTGGTGA TAGAACAGAT GCTGATGCCT CCCAAATATA CATGCAACTG 
TCAAAAAAAG ATCCTGTGAT AAAGCTCCTG TATGTCACCC CTGAGAAGGT CTGTGCGAGC 
AGCCGACTGA TGTCAGCCCT GGAGAACCTC TACAACAGGA AGCTCCTAGC ACGGTTTGTC 
ATCGATGAGG CCCACTGTGT CAGCCAGTGG GGTCACGACT TTCGACAAGA CTACAAGAGA 
CTGAACATGC TCCGCAGGAA GTTTCACGCT GTTCCCATGA TGGCCCTTAC TGCCACTGCC 
AACCCCAGAG TGCAAAAGGA TATCCAGAAT CAGCTTGAGA TGCTAAAGCC ACAAGTGTTT 
ACAATGAGCT TCAACAGGCA TAACTTGAAA TATGATGTAT TGCCCAAGAA GCCAAAGAAG 
GTGGCAATGG ATTGCTTGGA ATGGATTAAG AAATATCATC CATATGACTC TGGGATAATA 
TATTGCCTTT CCCGACACGA GTGTGACACC ACAGCAGCCA TTCTGCAGAA GGAGGGTCTC 
GCTGCACTTG CCTATCACGC TGGCCTCACC GACTCCAACA GGGATCTCGT GCAGAAGAAG 
TGGATTAACC AAGAGGGATG CCAGGTTATA TGTGCAACAA TTGCCTTTGG AATGGGAATC 
GACAAGCCTG ACGTGCGCTA CGTTATCCAC GCCTCTCTTC CTAAGTCCAT AGAAGGTTAC 
TACCAGGAGT CTGGCAGAGC TGGGCGGGAT GGGGAGATGT CCCACTGTGT CCTCTTCTAC 
AGCTACAGCG ATGTCACCAG ACTGCGGAGG CTAATCCTGA TGGAGAAAGA TGGGAACAGC 
CACACGAGGC AGACCCACTT CAACAACCTG TACAGCATGG TTCACTACTG CGAGAACGTG 
GTGGACTGCC GGAGAATCCA GCTTTTGGCC TACTTTGGGG AAACTGATTT TAATCCCACC 
TTCTGTAAAG ATCACCCAGA AGTAATCTGT GATAACTGCA GCAGGAAGAA GGATTACAAA 
TCAAGGGATG TAACAGATGA AGTGAAGAGC ATTATAAGAT TTGTACGAGA GCATTGTGGA 
CAAAACGGAC GAGCAAATGC AAAGAGAAAC GCAGGTTCTG GGAGATACAC CTTGAACATG 
ATGGTAGACA TTTTCCTAGG TACAGCAAGT GCGAAGATTC AGTCTGGAAT ATTTGGGAAG 
GGAGCTGCCT ACTCAAGGCA TAATGTGGAA AGGCTGTTTA GAAAACTGGT CCTGGACAAG 
ATCCTGGATG AAGACCTGTA TATCACAGCT AATGACCAAG CCGTGGCCTA TGTAATTCTG 
GGAGAGAAAG CCCAGGTTGT GCTAGATGGA TCACTACAGG TGGAGTTCCA TGAAACAGAA 
AGTGCCAGTG CCATCAGAAA GCAAAGGGCC TCCATGGCAA AAATGTCACA GCGGGAAGAG 
ATGGTCAAGA AGTGCCTCAG TGAGCTCACA GACACCTGCA AAACACTTGG GAAGGTCTTT 
GATGTGCATT ACTTCAATAT TTTCAGTACT TTGACGCTGA AGAAAATCGC AGAATCCTTG 
TCTTCTGATG CAGAGGTTTT ACTGCAGATT GATGGTGTCA CAGAAGACAA GCTGGAAAAA 
TACGGTGCAG AGATAATTAA AGTGATGGAG AAGTACTCTG AATGGACCAC ACCAGAAGAT 
GCTGGTGGCC AAAGTGTGGA CACAGCCCCG GGAAGTGCTG GCACACAAGG GAGTGATGAA 
GAGGCAGATG AAGGGACAAC CTCAACCTAT TTTTGCAATA ACACAAACCA AAGAAGGAAA 
AGAAAAAGGC CACCAAACTT CAAAGAATCC AAGAGGAAAA GGACAACTAA TGGTGGTGGC 
CAGCAGTTCC AGCACAGAGG TGGCTACAGC AAGTACAGAA GGACCAAGAG GCCGCTCAGC 
TCCAAGGCCC CGGCCCCATC CAGCAGCAGT GCAGCATGGC AGGGCCTCAG CACCACGCAG 
GGAGCTGCTG GGAAGCTGGG CATCATGGCA CTGCCCAAGC CCAAAACCAG ACACTTCCTC 
CAGCCTTCGT ATGCAGTACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010017129.1
CDS	31..3234
Translation	MEEICKLVEAIPLQELKSLSCAKDLLQKRDLRRKLLASSVGLNKSSFKCSFPRAWQVPVERGPPPCPGAAPCPGLSWSFCSDSNSPRATNPPSLLLGSAHSSKFSTPRNQTLDTSCASKQSVEEITCPGTTGLSSSRVNGAKGASLSLHPEVTLNSSCCERPGVRNSGIHPLLQRPLTSTALKGQSKAPTDVPTEDTSGINDDFDLDPFDIDDFDEGWEDVVDVSAPKGPSTPSYQPIREGPPAKSLLSKITSRAKGSAVVSNPAAPKSSSLMTTKNCSGPAVVNPALERFRGLKFSHSEEMMTIFHKKFGLHCFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSAGVTIVISPLRSLIIDQVQKLKTLDIAATYLTGDRTDADASQIYMQLSKKDPVIKLLYVTPEKVCASSRLMSALENLYNRKLLARFVIDEAHCVSQWGHDFRQDYKRLNMLRRKFHAVPMMALTATANPRVQKDIQNQLEMLKPQVFTMSFNRHNLKYDVLPKKPKKVAMDCLEWIKKYHPYDSGIIYCLSRHECDTTAAILQKEGLAALAYHAGLTDSNRDLVQKKWINQEGCQVICATIAFGMGIDKPDVRYVIHASLPKSIEGYYQESGRAGRDGEMSHCVLFYSYSDVTRLRRLILMEKDGNSHTRQTHFNNLYSMVHYCENVVDCRRIQLLAYFGETDFNPTFCKDHPEVICDNCSRKKDYKSRDVTDEVKSIIRFVREHCGQNGRANAKRNAGSGRYTLNMMVDIFLGTASAKIQSGIFGKGAAYSRHNVERLFRKLVLDKILDEDLYITANDQAVAYVILGEKAQVVLDGSLQVEFHETESASAIRKQRASMAKMSQREEMVKKCLSELTDTCKTLGKVFDVHYFNIFSTLTLKKIAESLSSDAEVLLQIDGVTEDKLEKYGAEIIKVMEKYSEWTTPEDAGGQSVDTAPGSAGTQGSDEEADEGTTSTYFCNNTNQRRKRKRPPNFKESKRKRTTNGGGQQFQHRGGYSKYRRTKRPLSSKAPAPSSSSAAWQGLSTTQGAAGKLGIMALPKPKTRHFLQPSYAVL

Target ORF information:

RefSeq Version	XM_010018827.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis Bloom syndrome, RecQ helicase-like (BLM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010018827.1

ORF Insert Sequence:

ATGGAGGAGA TCTGTAAGCT GGTAGAGGCC ATTCCACTGC AGGAGCTCAA GTCTCTGTCA 
TGTGCTAAAG ACCTTCTCCA GAAGCGAGAC CTCAGGAGAA AACTGCTCGC TAGTTCTGTT 
GGTTTGAACA AAAGTAGCTT CAAATGCAGC TTTCCCAGAG CCTGGCAGGT GCCTGTGGAG 
CGGGGCCCTC CCCCATGTCC TGGTGCTGCA CCATGTCCTG GCCTCAGCTG GAGCTTCTGT 
TCTGACAGCA ACTCGCCCAG AGCCACAAAC CCCCCATCCC TGCTGCTCGG TTCTGCTCAT 
TCAAGCAAGT TTTCTACGCC CAGAAATCAA ACCTTGGATA CCTCTTGTGC TTCAAAGCAA 
AGTGTTGAGG AGATCACCTG CCCAGGCACC ACGGGGCTGT CTTCCTCCAG GGTGAATGGT 
GCAAAGGGAG CTTCCCTCAG CCTCCATCCT GAGGTGACCC TCAACAGCTC CTGCTGTGAG 
CGCCCAGGGG TGAGGAACAG CGGAATCCAC CCTTTGCTGC AGAGGCCTCT TACAAGCACT 
GCTCTGAAGG GGCAGAGTAA GGCTCCCACT GACGTTCCCA CAGAAGACAC TTCAGGTATA 
AACGATGACT TTGACCTGGA CCCCTTCGAT ATTGATGATT TTGATGAGGG CTGGGAGGAC 
GTGGTGGATG TGTCGGCACC AAAGGGTCCA TCCACGCCGT CGTACCAGCC CATCAGGGAA 
GGGCCACCTG CCAAATCGCT GCTCTCAAAG ATAACGTCCA GGGCCAAGGG ATCTGCTGTT 
GTATCAAATC CTGCTGCTCC CAAGTCAAGC TCCTTGATGA CAACAAAGAA CTGCTCAGGT 
CCGGCAGTTG TTAACCCTGC ACTGGAGCGT TTCAGGGGTC TGAAATTTTC CCATTCTGAA 
GAAATGATGA CTATATTTCA CAAGAAGTTT GGGCTGCACT GCTTCAGGAC AAACCAGCTG 
GAGGCCATCA ACGCTGCTCT TCTGGGTGAA GACTGTTTCA TCTTAATGCC CACTGGTGGT 
GGTAAAAGCT TGTGCTACCA GTTACCAGCG TGTGTCTCTG CTGGAGTCAC TATTGTTATC 
TCTCCACTGA GGTCGCTGAT AATAGACCAG GTTCAGAAAC TGAAGACTTT AGATATTGCT 
GCAACATACC TGACTGGTGA TAGAACAGAT GCTGATGCCT CCCAAATATA CATGCAACTG 
TCAAAAAAAG ATCCTGTGAT AAAGCTCCTG TATGTCACCC CTGAGAAGGT CTGTGCGAGC 
AGCCGACTGA TGTCAGCCCT GGAGAACCTC TACAACAGGA AGCTCCTAGC ACGGTTTGTC 
ATCGATGAGG CCCACTGTGT CAGCCAGTGG GGTCACGACT TTCGACAAGA CTACAAGAGA 
CTGAACATGC TCCGCAGGAA GTTTCACGCT GTTCCCATGA TGGCCCTTAC TGCCACTGCC 
AACCCCAGAG TGCAAAAGGA TATCCAGAAT CAGCTTGAGA TGCTAAAGCC ACAAGTGTTT 
ACAATGAGCT TCAACAGGCA TAACTTGAAA TATGATGTAT TGCCCAAGAA GCCAAAGAAG 
GTGGCAATGG ATTGCTTGGA ATGGATTAAG AAATATCATC CATATGACTC TGGGATAATA 
TATTGCCTTT CCCGACACGA GTGTGACACC ACAGCAGCCA TTCTGCAGAA GGAGGGTCTC 
GCTGCACTTG CCTATCACGC TGGCCTCACC GACTCCAACA GGGATCTCGT GCAGAAGAAG 
TGGATTAACC AAGAGGGATG CCAGGTTATA TGTGCAACAA TTGCCTTTGG AATGGGAATC 
GACAAGCCTG ACGTGCGCTA CGTTATCCAC GCCTCTCTTC CTAAGTCCAT AGAAGGTTAC 
TACCAGGAGT CTGGCAGAGC TGGGCGGGAT GGGGAGATGT CCCACTGTGT CCTCTTCTAC 
AGCTACAGCG ATGTCACCAG ACTGCGGAGG CTAATCCTGA TGGAGAAAGA TGGGAACAGC 
CACACGAGGC AGACCCACTT CAACAACCTG TACAGCATGG TTCACTACTG CGAGAACGTG 
GTGGACTGCC GGAGAATCCA GCTTTTGGCC TACTTTGGGG AAACTGATTT TAATCCCACC 
TTCTGTAAAG ATCACCCAGA AGTAATCTGT GATAACTGCA GCAGGAAGAA GGATTACAAA 
TCAAGGGATG TAACAGATGA AGTGAAGAGC ATTATAAGAT TTGTACGAGA GCATTGTGGA 
CAAAACGGAC GAGCAAATGC AAAGAGAAAC GCAGGTTCTG GGAGATACAC CTTGAACATG 
ATGGTAGACA TTTTCCTAGG TACAGCAAGT GCGAAGATTC AGTCTGGAAT ATTTGGGAAG 
GGAGCTGCCT ACTCAAGGCA TAATGTGGAA AGGCTGTTTA GAAAACTGGT CCTGGACAAG 
ATCCTGGATG AAGACCTGTA TATCACAGCT AATGACCAAG CCGTGGCCTA TGTAATTCTG 
GGAGAGAAAG CCCAGGTTGT GCTAGATGGA TCACTACAGG TGGAGTTCCA TGAAACAGAA 
AGTGCCAGTG CCATCAGAAA GCAAAGGGCC TCCATGGCAA AAATGTCACA GCGGGAAGAG 
ATGGTCAAGA AGTGCCTCAG TGAGCTCACA GACACCTGCA AAACACTTGG GAAGGTCTTT 
GATGTGCATT ACTTCAATAT TTTCAGTACT TTGACGCTGA AGAAAATCGC AGAATCCTTG 
TCTTCTGATG CAGAGGTTTT ACTGCAGATT GATGGTGTCA CAGAAGACAA GCTGGAAAAA 
TACGGTGCAG AGATAATTAA AGTGATGGAG AAGTACTCTG AATGGACCAC ACCAGAAGAT 
GCTGGTGGCC AAAGTGTGGA CACAGCCCCG GGAAGTGCTG GCACACAAGG GAGTGATGAA 
GAGGCAGATG AAGGGACAAC CTCAACCTAT TTTTGCAATA ACACAAACCA AAGAAGGAAA 
AGAAAAAGGC CACCAAACTT CAAAGAATCC AAGAGGAAAA GGACAACTAA TGGTGGTGGC 
CAGCAGTTCC AGCACAGAGG TGGCTACAGC AAGTACAGAA GGACCAAGAG GCCGCTCAGC 
TCCAAGGCCC CGGCCCCATC CAGCAGCAGT GCAGCATGGC AGGGCCTCAG CACCACGCAG 
GGAGCTGCTG GGAAGCTGGG CATCATGGCA CTGCCCAAGC CCAAAACCAG ACACTTCCTC 
CAGCCTTCGT ATGCAGTACT GTAA