LOC107218920 cDNA ORF clone, Neodiprion lecontei(redheaded pine sawfly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107218920
Entrez Gene ID	107218920
Full Name	probable ATP-dependent RNA helicase kurz
Gene Type	protein-coding
Organism	Neodiprion lecontei(redheaded pine sawfly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015656960.1	XP_015512446.1	probable ATP-dependent RNA helicase kurz
XM_015656961.1	XP_015512447.1	probable ATP-dependent RNA helicase kurz

Pathways from BioSystems
Gene Ontology

The following LOC107218920 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107218920 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe15968	XM_015656960.1 Latest version!	Neodiprion lecontei probable ATP-dependent RNA helicase kurz (LOC107218920), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONe15968	XM_015656961.1 Latest version!	Neodiprion lecontei probable ATP-dependent RNA helicase kurz (LOC107218920), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107218920 ( XM_015656960.1 ) cDNA ORF clone, Neodiprion lecontei(redheaded pine sawfly) -> XP_015512446.1 Neodiprion lecontei probable ATP-dependent RNA helicase kurz (LOC107218920), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONe15968
Clone ID Related Accession (Same CDS sequence)	XM_015656960.1 , XM_015656961.1
Accession Version	XM_015656960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-18
Organism	Neodiprion lecontei(redheaded pine sawfly)
Product	probable ATP-dependent RNA helicase kurz
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015382794.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Neodiprion lecontei Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAA AAGGATACAA TTGGAAGGCA CGGAAGGTGA CGCAAATCGA AATTGACGAT 
ACTTTAACCA AAAAGATAGC AGTTGACATT GGATCTCAAC GTGAGAGTTA TGACCAGTGT 
AATGCTTTGG TTATCCCGAG CCAAAAGAGG AAAACTAAAT CCAAAGATAA AAAAATTACC 
ACAACTCGTT TGCTCTCGAA AAAACGTCGT AAAGAATTGG AAAAAGTTGT TGATCGAAAA 
AGAAAGAAAG CCAATAGGGC AACGCTTTTG GACAAGTTAG CTAAAGTCCA AGTCTCGGAA 
GAAGAGCTCA AACAGTACAC AACACTTGCG GCTGTACAAA CGAGAGGCTT AAAGCGCCAT 
TTCAATGAGG TGGACAAGGA GCTCAAGCCA AAACCACTCA CGTCTGAGCC TGAAGAGGAA 
CAAATAATCG TGAATGCTAT CAGAGGAGCC AAACAAACCA GACTTTCTCT TCTTGGTGCA 
GATGGAAAAC AAAATACGCA GTTTGATCCC AACATCGTCG GCTTTGATGA ATCTAGTGAT 
GATGAAACCA AAAGTGAAGA CGACTTTGAG CAGCCGAGCA GCATAGTTAA ACCTGCCGAA 
GAAAGTGTAA ATGCCAAAAT TCAACGAGTA AAAAATGATG TTACGGTAAA TAAACCACAT 
ACAACATCAG ATAAAAACCG AGATAAAATT CGCACCGACA AAGTTGCATT GAACAATGTT 
GACCCAGTGC AGCAAAAACC AGCTGTCTTT GTAATGTTGG ATCGCAAGCC AGAAATTCAG 
ACTGCACGGC TGAAGCTTCC CATTTTAGCG GAAGAGCAGT CAATCATGGA AACAATCAAT 
GACAATCCTG TGGTGATTGT TGCTGGTGAA ACGGGAAGTG GTAAAACGAC CCAAGTGCCA 
CAATTCTTGT ATGAAGCTGG CTATGCAAAA GAGAAAATGA TCGGTGTCAC AGAGCCAAGA 
CGAGTCGCAG CAATTTCTAT GAGCAAACGT GTCGCAGAGG AAATGAATCT GTCATCTAAA 
GAAGTGTCGT ATCTCATTAG ATTCGAAGGC AACACTACTT CTGATACCAA AATTAAATTC 
ATGACCGACG GTGTATTGCT AAAGGAAATT CAAAGCGACT TTCTTCTCAA CAAATATTCG 
GTTATTATTT TGGATGAAGC ACACGAACGA AGTGTCTACA CAGATATCTT GATTGGTTTA 
TTGTCGCGAA TCGTTCCTTT GCGAAATAAA AGGAAATCTC CATTGAAATT AATTGTAATG 
TCTGCAACAT TGCGCATCGA AGATTTCACA GAAAATCCTA AACTCTTCAA AGTCAAACCA 
CCAGTCATCA AAGTTGAATC AAGGCAGTTT CCTGTTACTA TACATTTCAA TAAAAGGACT 
AGTCAAAATT ACGTGAAGGA TGCTTTGTAC AAGGCAGTAA AGATTCATAC TCAGCTGCCG 
GATGGAGGTA TTCTTATATT CTTAACAGGG CAGCAGGAAG TAAACACAAT AGTGCGCAAA 
CTACGTAAAG CGTTTCCTTT AAGAAACAAA AAATTCATGC TCAAAAAACA AGAAGCAAAA 
GAAATCCAAC AAGAGAAAGA ACCTAGCAGC AACAACAGCG AGTCAGACGA GGGTTTGGAT 
ATGGATCGAG TGGTAAAAAA TGCCAGGAAA CATAGAAAAA AGCTGAAGGA GGTCAAACTC 
CCTGAACTTA ATCTTGATAA TTATGCTGTA ATGCCTCCAG ATGATACTGA GGGTGATCTA 
CTCGATACAG TAGAAAATCG GGATGCAGAA GACAAAGAAG AAGAAGAAGA TGATGATGAT 
GATGATGAAG AAGAAGATTT ACAAAATCTT ATGGGACTGT CGTGCACACA GCCAATGTGG 
GTGTTACCAC TGTATTCGCT TCTTCCGAGT TACAAACAAG CAAAGGTATT TGATCCACCA 
CCTGAAGGCT GTCGATTATG TGTTGTATCG ACAAATGTTG CAGAAACTTC CCTCACAATT 
CCGAATGTAA AATACGTTGT GGATAGCGGC CGCTGCAAGA CCAGATTATA TGACAAAGTG 
ACGGGTGTTA ACGCGTTCCA AGTATGTTAC ACTAGTAAAG CAGCTGCTAA TCAGCGGGCT 
GGACGAGCAG GTAGAACTGG ACCGGGACAT TGTTACAGAT TATATTCATC AGCGGTTTTT 
AATGACCAGT TTGAGCAATT TTCTATGCCT GAAATACAGA GGAAACCGGT AGATGACTTG 
TTATTGCAAA TGAAAGTCAT GAATATTGAT AAAGTCGTCA ATTTTCCATT CCCATCATCA 
CCAGATGTGT TACAATTGAA ATCTGCTGAA AAACGTCTCT GCATTCTCGG AGCTCTAGAT 
CAATCTAAAC CTGGCAAAGA GAATGAATAT TGTGCCAAAG TAACGCCACT TGGCTACAGC 
ATAGCAGCGT TCCCAGTGGC ACCTCGATTT GGTAAAATGC TGGCGCTATC ACAGCAGCAC 
GATCTGTTAC AATACACAGT TTGCTTGGTG GCTGCTTTGT CTGTGCAAGA AGTGTTGATT 
GAATCAAATC CAATTCAAGG CGTTACTAAG AATCAGTGGA ATCAAACACG TCGAACTTGG 
GCAGGAACTG GGAACAGTTT ACTTCTGGGG GATGCGATGG TCTTAGTTCG AGCCGTTGGT 
GCTGCAGAAT ATGCTGGGTC AAAAGGGACG ATAGTCAAGT TTTGCGAAGA AAATGGACTA 
CGATACAAGG CTGTGACAGA AATTAGAAAA CTCAGGCAAC AACTGACCAA TGAAATAGTC 
CTAAATATAC CAGATTTGGA TTTGAATGTC AATCCAAGAA TGAATCCTCC AACGGATACA 
CAAGCCAAAT TACTTAGGCA AATTCTACTT TCTGGTATGG CAAATCAAGT CGCACGCAAA 
GTTTCTCCAG ATGAAGTTGA GGAAGATCAC GACAAAGTAA AGTGGAAATA TGCATACAGA 
TGTTTGGAAA TGGAAGATCC AGTGTTCATG CACTCTTCGT CTGTTCTAAA AAAAACTTAC 
CCTGAATGGG TAGTTTATCA GGAGGTTTAT GAAACTAATA AAATATACAT GCGAGGCATT 
ACTGCCATTC AGCCAGAATG GTTACCAAAA TTTGCCACAT CCTTATGCCA ACTGAGCGAA 
CCTGTGAGCG ACCCGCCGCC TAGGTACGAT TCAAGAACGG GAAAGGTGAT GTGCCATGTA 
ACAGGGACAT TCGGTCGAGG TGGATGGCAA CTGCCGCTGA TTGAAGTAGA GTATCCAAAT 
TCCCTGGAAG GTATCAAGTG GTTTGCCCGA TTTTTTTTGG AAGGTCAAGT TTGCCCGGCA 
TTGGAACGAT TTGCTTCCTT GCTACTTTCT ACACCTGACA CAATGAACAA GACATGGGCA 
AAATTACTCC CGCGAACTGA AGCAATCACA AAAGCGCTAA TGGCTAATCA AGTTTCGTCA 
AAAGATCAAC TGTATCACAT TTGGAAAACG GATTCGAAAT TTCTTCTGAA TGCATACCAA 
AAGTGGTTGC CAGAATCGGC GCAGAATGAA GTTGCAATAC TTTGGCCACC GTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015512446.1
CDS	5..3541
Translation	MGKKGYNWKARKVTQIEIDDTLTKKIAVDIGSQRESYDQCNALVIPSQKRKTKSKDKKITTTRLLSKKRRKELEKVVDRKRKKANRATLLDKLAKVQVSEEELKQYTTLAAVQTRGLKRHFNEVDKELKPKPLTSEPEEEQIIVNAIRGAKQTRLSLLGADGKQNTQFDPNIVGFDESSDDETKSEDDFEQPSSIVKPAEESVNAKIQRVKNDVTVNKPHTTSDKNRDKIRTDKVALNNVDPVQQKPAVFVMLDRKPEIQTARLKLPILAEEQSIMETINDNPVVIVAGETGSGKTTQVPQFLYEAGYAKEKMIGVTEPRRVAAISMSKRVAEEMNLSSKEVSYLIRFEGNTTSDTKIKFMTDGVLLKEIQSDFLLNKYSVIILDEAHERSVYTDILIGLLSRIVPLRNKRKSPLKLIVMSATLRIEDFTENPKLFKVKPPVIKVESRQFPVTIHFNKRTSQNYVKDALYKAVKIHTQLPDGGILIFLTGQQEVNTIVRKLRKAFPLRNKKFMLKKQEAKEIQQEKEPSSNNSESDEGLDMDRVVKNARKHRKKLKEVKLPELNLDNYAVMPPDDTEGDLLDTVENRDAEDKEEEEDDDDDDEEEDLQNLMGLSCTQPMWVLPLYSLLPSYKQAKVFDPPPEGCRLCVVSTNVAETSLTIPNVKYVVDSGRCKTRLYDKVTGVNAFQVCYTSKAAANQRAGRAGRTGPGHCYRLYSSAVFNDQFEQFSMPEIQRKPVDDLLLQMKVMNIDKVVNFPFPSSPDVLQLKSAEKRLCILGALDQSKPGKENEYCAKVTPLGYSIAAFPVAPRFGKMLALSQQHDLLQYTVCLVAALSVQEVLIESNPIQGVTKNQWNQTRRTWAGTGNSLLLGDAMVLVRAVGAAEYAGSKGTIVKFCEENGLRYKAVTEIRKLRQQLTNEIVLNIPDLDLNVNPRMNPPTDTQAKLLRQILLSGMANQVARKVSPDEVEEDHDKVKWKYAYRCLEMEDPVFMHSSSVLKKTYPEWVVYQEVYETNKIYMRGITAIQPEWLPKFATSLCQLSEPVSDPPPRYDSRTGKVMCHVTGTFGRGGWQLPLIEVEYPNSLEGIKWFARFFLEGQVCPALERFASLLLSTPDTMNKTWAKLLPRTEAITKALMANQVSSKDQLYHIWKTDSKFLLNAYQKWLPESAQNEVAILWPPL

Target ORF information:

RefSeq Version	XM_015656960.1
Organism	Neodiprion lecontei(redheaded pine sawfly)
Definition	Neodiprion lecontei probable ATP-dependent RNA helicase kurz (LOC107218920), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015656960.1

ORF Insert Sequence:

ATGGGTAAAA AAGGATACAA TTGGAAGGCA CGGAAGGTGA CGCAAATCGA AATTGACGAT 
ACTTTAACCA AAAAGATAGC AGTTGACATT GGATCTCAAC GTGAGAGTTA TGACCAGTGT 
AATGCTTTGG TTATCCCGAG CCAAAAGAGG AAAACTAAAT CCAAAGATAA AAAAATTACC 
ACAACTCGTT TGCTCTCGAA AAAACGTCGT AAAGAATTGG AAAAAGTTGT TGATCGAAAA 
AGAAAGAAAG CCAATAGGGC AACGCTTTTG GACAAGTTAG CTAAAGTCCA AGTCTCGGAA 
GAAGAGCTCA AACAGTACAC AACACTTGCG GCTGTACAAA CGAGAGGCTT AAAGCGCCAT 
TTCAATGAGG TGGACAAGGA GCTCAAGCCA AAACCACTCA CGTCTGAGCC TGAAGAGGAA 
CAAATAATCG TGAATGCTAT CAGAGGAGCC AAACAAACCA GACTTTCTCT TCTTGGTGCA 
GATGGAAAAC AAAATACGCA GTTTGATCCC AACATCGTCG GCTTTGATGA ATCTAGTGAT 
GATGAAACCA AAAGTGAAGA CGACTTTGAG CAGCCGAGCA GCATAGTTAA ACCTGCCGAA 
GAAAGTGTAA ATGCCAAAAT TCAACGAGTA AAAAATGATG TTACGGTAAA TAAACCACAT 
ACAACATCAG ATAAAAACCG AGATAAAATT CGCACCGACA AAGTTGCATT GAACAATGTT 
GACCCAGTGC AGCAAAAACC AGCTGTCTTT GTAATGTTGG ATCGCAAGCC AGAAATTCAG 
ACTGCACGGC TGAAGCTTCC CATTTTAGCG GAAGAGCAGT CAATCATGGA AACAATCAAT 
GACAATCCTG TGGTGATTGT TGCTGGTGAA ACGGGAAGTG GTAAAACGAC CCAAGTGCCA 
CAATTCTTGT ATGAAGCTGG CTATGCAAAA GAGAAAATGA TCGGTGTCAC AGAGCCAAGA 
CGAGTCGCAG CAATTTCTAT GAGCAAACGT GTCGCAGAGG AAATGAATCT GTCATCTAAA 
GAAGTGTCGT ATCTCATTAG ATTCGAAGGC AACACTACTT CTGATACCAA AATTAAATTC 
ATGACCGACG GTGTATTGCT AAAGGAAATT CAAAGCGACT TTCTTCTCAA CAAATATTCG 
GTTATTATTT TGGATGAAGC ACACGAACGA AGTGTCTACA CAGATATCTT GATTGGTTTA 
TTGTCGCGAA TCGTTCCTTT GCGAAATAAA AGGAAATCTC CATTGAAATT AATTGTAATG 
TCTGCAACAT TGCGCATCGA AGATTTCACA GAAAATCCTA AACTCTTCAA AGTCAAACCA 
CCAGTCATCA AAGTTGAATC AAGGCAGTTT CCTGTTACTA TACATTTCAA TAAAAGGACT 
AGTCAAAATT ACGTGAAGGA TGCTTTGTAC AAGGCAGTAA AGATTCATAC TCAGCTGCCG 
GATGGAGGTA TTCTTATATT CTTAACAGGG CAGCAGGAAG TAAACACAAT AGTGCGCAAA 
CTACGTAAAG CGTTTCCTTT AAGAAACAAA AAATTCATGC TCAAAAAACA AGAAGCAAAA 
GAAATCCAAC AAGAGAAAGA ACCTAGCAGC AACAACAGCG AGTCAGACGA GGGTTTGGAT 
ATGGATCGAG TGGTAAAAAA TGCCAGGAAA CATAGAAAAA AGCTGAAGGA GGTCAAACTC 
CCTGAACTTA ATCTTGATAA TTATGCTGTA ATGCCTCCAG ATGATACTGA GGGTGATCTA 
CTCGATACAG TAGAAAATCG GGATGCAGAA GACAAAGAAG AAGAAGAAGA TGATGATGAT 
GATGATGAAG AAGAAGATTT ACAAAATCTT ATGGGACTGT CGTGCACACA GCCAATGTGG 
GTGTTACCAC TGTATTCGCT TCTTCCGAGT TACAAACAAG CAAAGGTATT TGATCCACCA 
CCTGAAGGCT GTCGATTATG TGTTGTATCG ACAAATGTTG CAGAAACTTC CCTCACAATT 
CCGAATGTAA AATACGTTGT GGATAGCGGC CGCTGCAAGA CCAGATTATA TGACAAAGTG 
ACGGGTGTTA ACGCGTTCCA AGTATGTTAC ACTAGTAAAG CAGCTGCTAA TCAGCGGGCT 
GGACGAGCAG GTAGAACTGG ACCGGGACAT TGTTACAGAT TATATTCATC AGCGGTTTTT 
AATGACCAGT TTGAGCAATT TTCTATGCCT GAAATACAGA GGAAACCGGT AGATGACTTG 
TTATTGCAAA TGAAAGTCAT GAATATTGAT AAAGTCGTCA ATTTTCCATT CCCATCATCA 
CCAGATGTGT TACAATTGAA ATCTGCTGAA AAACGTCTCT GCATTCTCGG AGCTCTAGAT 
CAATCTAAAC CTGGCAAAGA GAATGAATAT TGTGCCAAAG TAACGCCACT TGGCTACAGC 
ATAGCAGCGT TCCCAGTGGC ACCTCGATTT GGTAAAATGC TGGCGCTATC ACAGCAGCAC 
GATCTGTTAC AATACACAGT TTGCTTGGTG GCTGCTTTGT CTGTGCAAGA AGTGTTGATT 
GAATCAAATC CAATTCAAGG CGTTACTAAG AATCAGTGGA ATCAAACACG TCGAACTTGG 
GCAGGAACTG GGAACAGTTT ACTTCTGGGG GATGCGATGG TCTTAGTTCG AGCCGTTGGT 
GCTGCAGAAT ATGCTGGGTC AAAAGGGACG ATAGTCAAGT TTTGCGAAGA AAATGGACTA 
CGATACAAGG CTGTGACAGA AATTAGAAAA CTCAGGCAAC AACTGACCAA TGAAATAGTC 
CTAAATATAC CAGATTTGGA TTTGAATGTC AATCCAAGAA TGAATCCTCC AACGGATACA 
CAAGCCAAAT TACTTAGGCA AATTCTACTT TCTGGTATGG CAAATCAAGT CGCACGCAAA 
GTTTCTCCAG ATGAAGTTGA GGAAGATCAC GACAAAGTAA AGTGGAAATA TGCATACAGA 
TGTTTGGAAA TGGAAGATCC AGTGTTCATG CACTCTTCGT CTGTTCTAAA AAAAACTTAC 
CCTGAATGGG TAGTTTATCA GGAGGTTTAT GAAACTAATA AAATATACAT GCGAGGCATT 
ACTGCCATTC AGCCAGAATG GTTACCAAAA TTTGCCACAT CCTTATGCCA ACTGAGCGAA 
CCTGTGAGCG ACCCGCCGCC TAGGTACGAT TCAAGAACGG GAAAGGTGAT GTGCCATGTA 
ACAGGGACAT TCGGTCGAGG TGGATGGCAA CTGCCGCTGA TTGAAGTAGA GTATCCAAAT 
TCCCTGGAAG GTATCAAGTG GTTTGCCCGA TTTTTTTTGG AAGGTCAAGT TTGCCCGGCA 
TTGGAACGAT TTGCTTCCTT GCTACTTTCT ACACCTGACA CAATGAACAA GACATGGGCA 
AAATTACTCC CGCGAACTGA AGCAATCACA AAAGCGCTAA TGGCTAATCA AGTTTCGTCA 
AAAGATCAAC TGTATCACAT TTGGAAAACG GATTCGAAAT TTCTTCTGAA TGCATACCAA 
AAGTGGTTGC CAGAATCGGC GCAGAATGAA GTTGCAATAC TTTGGCCACC GTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107218920 ( XM_015656961.1 ) cDNA ORF clone, Neodiprion lecontei(redheaded pine sawfly) -> XP_015512447.1 Neodiprion lecontei probable ATP-dependent RNA helicase kurz (LOC107218920), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONe15968
Clone ID Related Accession (Same CDS sequence)	XM_015656960.1 , XM_015656961.1
Accession Version	XM_015656961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-18
Organism	Neodiprion lecontei(redheaded pine sawfly)
Product	probable ATP-dependent RNA helicase kurz
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015382794.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Neodiprion lecontei Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAAAA AAGGATACAA TTGGAAGGCA CGGAAGGTGA CGCAAATCGA AATTGACGAT 
ACTTTAACCA AAAAGATAGC AGTTGACATT GGATCTCAAC GTGAGAGTTA TGACCAGTGT 
AATGCTTTGG TTATCCCGAG CCAAAAGAGG AAAACTAAAT CCAAAGATAA AAAAATTACC 
ACAACTCGTT TGCTCTCGAA AAAACGTCGT AAAGAATTGG AAAAAGTTGT TGATCGAAAA 
AGAAAGAAAG CCAATAGGGC AACGCTTTTG GACAAGTTAG CTAAAGTCCA AGTCTCGGAA 
GAAGAGCTCA AACAGTACAC AACACTTGCG GCTGTACAAA CGAGAGGCTT AAAGCGCCAT 
TTCAATGAGG TGGACAAGGA GCTCAAGCCA AAACCACTCA CGTCTGAGCC TGAAGAGGAA 
CAAATAATCG TGAATGCTAT CAGAGGAGCC AAACAAACCA GACTTTCTCT TCTTGGTGCA 
GATGGAAAAC AAAATACGCA GTTTGATCCC AACATCGTCG GCTTTGATGA ATCTAGTGAT 
GATGAAACCA AAAGTGAAGA CGACTTTGAG CAGCCGAGCA GCATAGTTAA ACCTGCCGAA 
GAAAGTGTAA ATGCCAAAAT TCAACGAGTA AAAAATGATG TTACGGTAAA TAAACCACAT 
ACAACATCAG ATAAAAACCG AGATAAAATT CGCACCGACA AAGTTGCATT GAACAATGTT 
GACCCAGTGC AGCAAAAACC AGCTGTCTTT GTAATGTTGG ATCGCAAGCC AGAAATTCAG 
ACTGCACGGC TGAAGCTTCC CATTTTAGCG GAAGAGCAGT CAATCATGGA AACAATCAAT 
GACAATCCTG TGGTGATTGT TGCTGGTGAA ACGGGAAGTG GTAAAACGAC CCAAGTGCCA 
CAATTCTTGT ATGAAGCTGG CTATGCAAAA GAGAAAATGA TCGGTGTCAC AGAGCCAAGA 
CGAGTCGCAG CAATTTCTAT GAGCAAACGT GTCGCAGAGG AAATGAATCT GTCATCTAAA 
GAAGTGTCGT ATCTCATTAG ATTCGAAGGC AACACTACTT CTGATACCAA AATTAAATTC 
ATGACCGACG GTGTATTGCT AAAGGAAATT CAAAGCGACT TTCTTCTCAA CAAATATTCG 
GTTATTATTT TGGATGAAGC ACACGAACGA AGTGTCTACA CAGATATCTT GATTGGTTTA 
TTGTCGCGAA TCGTTCCTTT GCGAAATAAA AGGAAATCTC CATTGAAATT AATTGTAATG 
TCTGCAACAT TGCGCATCGA AGATTTCACA GAAAATCCTA AACTCTTCAA AGTCAAACCA 
CCAGTCATCA AAGTTGAATC AAGGCAGTTT CCTGTTACTA TACATTTCAA TAAAAGGACT 
AGTCAAAATT ACGTGAAGGA TGCTTTGTAC AAGGCAGTAA AGATTCATAC TCAGCTGCCG 
GATGGAGGTA TTCTTATATT CTTAACAGGG CAGCAGGAAG TAAACACAAT AGTGCGCAAA 
CTACGTAAAG CGTTTCCTTT AAGAAACAAA AAATTCATGC TCAAAAAACA AGAAGCAAAA 
GAAATCCAAC AAGAGAAAGA ACCTAGCAGC AACAACAGCG AGTCAGACGA GGGTTTGGAT 
ATGGATCGAG TGGTAAAAAA TGCCAGGAAA CATAGAAAAA AGCTGAAGGA GGTCAAACTC 
CCTGAACTTA ATCTTGATAA TTATGCTGTA ATGCCTCCAG ATGATACTGA GGGTGATCTA 
CTCGATACAG TAGAAAATCG GGATGCAGAA GACAAAGAAG AAGAAGAAGA TGATGATGAT 
GATGATGAAG AAGAAGATTT ACAAAATCTT ATGGGACTGT CGTGCACACA GCCAATGTGG 
GTGTTACCAC TGTATTCGCT TCTTCCGAGT TACAAACAAG CAAAGGTATT TGATCCACCA 
CCTGAAGGCT GTCGATTATG TGTTGTATCG ACAAATGTTG CAGAAACTTC CCTCACAATT 
CCGAATGTAA AATACGTTGT GGATAGCGGC CGCTGCAAGA CCAGATTATA TGACAAAGTG 
ACGGGTGTTA ACGCGTTCCA AGTATGTTAC ACTAGTAAAG CAGCTGCTAA TCAGCGGGCT 
GGACGAGCAG GTAGAACTGG ACCGGGACAT TGTTACAGAT TATATTCATC AGCGGTTTTT 
AATGACCAGT TTGAGCAATT TTCTATGCCT GAAATACAGA GGAAACCGGT AGATGACTTG 
TTATTGCAAA TGAAAGTCAT GAATATTGAT AAAGTCGTCA ATTTTCCATT CCCATCATCA 
CCAGATGTGT TACAATTGAA ATCTGCTGAA AAACGTCTCT GCATTCTCGG AGCTCTAGAT 
CAATCTAAAC CTGGCAAAGA GAATGAATAT TGTGCCAAAG TAACGCCACT TGGCTACAGC 
ATAGCAGCGT TCCCAGTGGC ACCTCGATTT GGTAAAATGC TGGCGCTATC ACAGCAGCAC 
GATCTGTTAC AATACACAGT TTGCTTGGTG GCTGCTTTGT CTGTGCAAGA AGTGTTGATT 
GAATCAAATC CAATTCAAGG CGTTACTAAG AATCAGTGGA ATCAAACACG TCGAACTTGG 
GCAGGAACTG GGAACAGTTT ACTTCTGGGG GATGCGATGG TCTTAGTTCG AGCCGTTGGT 
GCTGCAGAAT ATGCTGGGTC AAAAGGGACG ATAGTCAAGT TTTGCGAAGA AAATGGACTA 
CGATACAAGG CTGTGACAGA AATTAGAAAA CTCAGGCAAC AACTGACCAA TGAAATAGTC 
CTAAATATAC CAGATTTGGA TTTGAATGTC AATCCAAGAA TGAATCCTCC AACGGATACA 
CAAGCCAAAT TACTTAGGCA AATTCTACTT TCTGGTATGG CAAATCAAGT CGCACGCAAA 
GTTTCTCCAG ATGAAGTTGA GGAAGATCAC GACAAAGTAA AGTGGAAATA TGCATACAGA 
TGTTTGGAAA TGGAAGATCC AGTGTTCATG CACTCTTCGT CTGTTCTAAA AAAAACTTAC 
CCTGAATGGG TAGTTTATCA GGAGGTTTAT GAAACTAATA AAATATACAT GCGAGGCATT 
ACTGCCATTC AGCCAGAATG GTTACCAAAA TTTGCCACAT CCTTATGCCA ACTGAGCGAA 
CCTGTGAGCG ACCCGCCGCC TAGGTACGAT TCAAGAACGG GAAAGGTGAT GTGCCATGTA 
ACAGGGACAT TCGGTCGAGG TGGATGGCAA CTGCCGCTGA TTGAAGTAGA GTATCCAAAT 
TCCCTGGAAG GTATCAAGTG GTTTGCCCGA TTTTTTTTGG AAGGTCAAGT TTGCCCGGCA 
TTGGAACGAT TTGCTTCCTT GCTACTTTCT ACACCTGACA CAATGAACAA GACATGGGCA 
AAATTACTCC CGCGAACTGA AGCAATCACA AAAGCGCTAA TGGCTAATCA AGTTTCGTCA 
AAAGATCAAC TGTATCACAT TTGGAAAACG GATTCGAAAT TTCTTCTGAA TGCATACCAA 
AAGTGGTTGC CAGAATCGGC GCAGAATGAA GTTGCAATAC TTTGGCCACC GTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015512447.1
CDS	5..3541
Translation	MGKKGYNWKARKVTQIEIDDTLTKKIAVDIGSQRESYDQCNALVIPSQKRKTKSKDKKITTTRLLSKKRRKELEKVVDRKRKKANRATLLDKLAKVQVSEEELKQYTTLAAVQTRGLKRHFNEVDKELKPKPLTSEPEEEQIIVNAIRGAKQTRLSLLGADGKQNTQFDPNIVGFDESSDDETKSEDDFEQPSSIVKPAEESVNAKIQRVKNDVTVNKPHTTSDKNRDKIRTDKVALNNVDPVQQKPAVFVMLDRKPEIQTARLKLPILAEEQSIMETINDNPVVIVAGETGSGKTTQVPQFLYEAGYAKEKMIGVTEPRRVAAISMSKRVAEEMNLSSKEVSYLIRFEGNTTSDTKIKFMTDGVLLKEIQSDFLLNKYSVIILDEAHERSVYTDILIGLLSRIVPLRNKRKSPLKLIVMSATLRIEDFTENPKLFKVKPPVIKVESRQFPVTIHFNKRTSQNYVKDALYKAVKIHTQLPDGGILIFLTGQQEVNTIVRKLRKAFPLRNKKFMLKKQEAKEIQQEKEPSSNNSESDEGLDMDRVVKNARKHRKKLKEVKLPELNLDNYAVMPPDDTEGDLLDTVENRDAEDKEEEEDDDDDDEEEDLQNLMGLSCTQPMWVLPLYSLLPSYKQAKVFDPPPEGCRLCVVSTNVAETSLTIPNVKYVVDSGRCKTRLYDKVTGVNAFQVCYTSKAAANQRAGRAGRTGPGHCYRLYSSAVFNDQFEQFSMPEIQRKPVDDLLLQMKVMNIDKVVNFPFPSSPDVLQLKSAEKRLCILGALDQSKPGKENEYCAKVTPLGYSIAAFPVAPRFGKMLALSQQHDLLQYTVCLVAALSVQEVLIESNPIQGVTKNQWNQTRRTWAGTGNSLLLGDAMVLVRAVGAAEYAGSKGTIVKFCEENGLRYKAVTEIRKLRQQLTNEIVLNIPDLDLNVNPRMNPPTDTQAKLLRQILLSGMANQVARKVSPDEVEEDHDKVKWKYAYRCLEMEDPVFMHSSSVLKKTYPEWVVYQEVYETNKIYMRGITAIQPEWLPKFATSLCQLSEPVSDPPPRYDSRTGKVMCHVTGTFGRGGWQLPLIEVEYPNSLEGIKWFARFFLEGQVCPALERFASLLLSTPDTMNKTWAKLLPRTEAITKALMANQVSSKDQLYHIWKTDSKFLLNAYQKWLPESAQNEVAILWPPL

Target ORF information:

RefSeq Version	XM_015656961.1
Organism	Neodiprion lecontei(redheaded pine sawfly)
Definition	Neodiprion lecontei probable ATP-dependent RNA helicase kurz (LOC107218920), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015656961.1

ORF Insert Sequence:

ATGGGTAAAA AAGGATACAA TTGGAAGGCA CGGAAGGTGA CGCAAATCGA AATTGACGAT 
ACTTTAACCA AAAAGATAGC AGTTGACATT GGATCTCAAC GTGAGAGTTA TGACCAGTGT 
AATGCTTTGG TTATCCCGAG CCAAAAGAGG AAAACTAAAT CCAAAGATAA AAAAATTACC 
ACAACTCGTT TGCTCTCGAA AAAACGTCGT AAAGAATTGG AAAAAGTTGT TGATCGAAAA 
AGAAAGAAAG CCAATAGGGC AACGCTTTTG GACAAGTTAG CTAAAGTCCA AGTCTCGGAA 
GAAGAGCTCA AACAGTACAC AACACTTGCG GCTGTACAAA CGAGAGGCTT AAAGCGCCAT 
TTCAATGAGG TGGACAAGGA GCTCAAGCCA AAACCACTCA CGTCTGAGCC TGAAGAGGAA 
CAAATAATCG TGAATGCTAT CAGAGGAGCC AAACAAACCA GACTTTCTCT TCTTGGTGCA 
GATGGAAAAC AAAATACGCA GTTTGATCCC AACATCGTCG GCTTTGATGA ATCTAGTGAT 
GATGAAACCA AAAGTGAAGA CGACTTTGAG CAGCCGAGCA GCATAGTTAA ACCTGCCGAA 
GAAAGTGTAA ATGCCAAAAT TCAACGAGTA AAAAATGATG TTACGGTAAA TAAACCACAT 
ACAACATCAG ATAAAAACCG AGATAAAATT CGCACCGACA AAGTTGCATT GAACAATGTT 
GACCCAGTGC AGCAAAAACC AGCTGTCTTT GTAATGTTGG ATCGCAAGCC AGAAATTCAG 
ACTGCACGGC TGAAGCTTCC CATTTTAGCG GAAGAGCAGT CAATCATGGA AACAATCAAT 
GACAATCCTG TGGTGATTGT TGCTGGTGAA ACGGGAAGTG GTAAAACGAC CCAAGTGCCA 
CAATTCTTGT ATGAAGCTGG CTATGCAAAA GAGAAAATGA TCGGTGTCAC AGAGCCAAGA 
CGAGTCGCAG CAATTTCTAT GAGCAAACGT GTCGCAGAGG AAATGAATCT GTCATCTAAA 
GAAGTGTCGT ATCTCATTAG ATTCGAAGGC AACACTACTT CTGATACCAA AATTAAATTC 
ATGACCGACG GTGTATTGCT AAAGGAAATT CAAAGCGACT TTCTTCTCAA CAAATATTCG 
GTTATTATTT TGGATGAAGC ACACGAACGA AGTGTCTACA CAGATATCTT GATTGGTTTA 
TTGTCGCGAA TCGTTCCTTT GCGAAATAAA AGGAAATCTC CATTGAAATT AATTGTAATG 
TCTGCAACAT TGCGCATCGA AGATTTCACA GAAAATCCTA AACTCTTCAA AGTCAAACCA 
CCAGTCATCA AAGTTGAATC AAGGCAGTTT CCTGTTACTA TACATTTCAA TAAAAGGACT 
AGTCAAAATT ACGTGAAGGA TGCTTTGTAC AAGGCAGTAA AGATTCATAC TCAGCTGCCG 
GATGGAGGTA TTCTTATATT CTTAACAGGG CAGCAGGAAG TAAACACAAT AGTGCGCAAA 
CTACGTAAAG CGTTTCCTTT AAGAAACAAA AAATTCATGC TCAAAAAACA AGAAGCAAAA 
GAAATCCAAC AAGAGAAAGA ACCTAGCAGC AACAACAGCG AGTCAGACGA GGGTTTGGAT 
ATGGATCGAG TGGTAAAAAA TGCCAGGAAA CATAGAAAAA AGCTGAAGGA GGTCAAACTC 
CCTGAACTTA ATCTTGATAA TTATGCTGTA ATGCCTCCAG ATGATACTGA GGGTGATCTA 
CTCGATACAG TAGAAAATCG GGATGCAGAA GACAAAGAAG AAGAAGAAGA TGATGATGAT 
GATGATGAAG AAGAAGATTT ACAAAATCTT ATGGGACTGT CGTGCACACA GCCAATGTGG 
GTGTTACCAC TGTATTCGCT TCTTCCGAGT TACAAACAAG CAAAGGTATT TGATCCACCA 
CCTGAAGGCT GTCGATTATG TGTTGTATCG ACAAATGTTG CAGAAACTTC CCTCACAATT 
CCGAATGTAA AATACGTTGT GGATAGCGGC CGCTGCAAGA CCAGATTATA TGACAAAGTG 
ACGGGTGTTA ACGCGTTCCA AGTATGTTAC ACTAGTAAAG CAGCTGCTAA TCAGCGGGCT 
GGACGAGCAG GTAGAACTGG ACCGGGACAT TGTTACAGAT TATATTCATC AGCGGTTTTT 
AATGACCAGT TTGAGCAATT TTCTATGCCT GAAATACAGA GGAAACCGGT AGATGACTTG 
TTATTGCAAA TGAAAGTCAT GAATATTGAT AAAGTCGTCA ATTTTCCATT CCCATCATCA 
CCAGATGTGT TACAATTGAA ATCTGCTGAA AAACGTCTCT GCATTCTCGG AGCTCTAGAT 
CAATCTAAAC CTGGCAAAGA GAATGAATAT TGTGCCAAAG TAACGCCACT TGGCTACAGC 
ATAGCAGCGT TCCCAGTGGC ACCTCGATTT GGTAAAATGC TGGCGCTATC ACAGCAGCAC 
GATCTGTTAC AATACACAGT TTGCTTGGTG GCTGCTTTGT CTGTGCAAGA AGTGTTGATT 
GAATCAAATC CAATTCAAGG CGTTACTAAG AATCAGTGGA ATCAAACACG TCGAACTTGG 
GCAGGAACTG GGAACAGTTT ACTTCTGGGG GATGCGATGG TCTTAGTTCG AGCCGTTGGT 
GCTGCAGAAT ATGCTGGGTC AAAAGGGACG ATAGTCAAGT TTTGCGAAGA AAATGGACTA 
CGATACAAGG CTGTGACAGA AATTAGAAAA CTCAGGCAAC AACTGACCAA TGAAATAGTC 
CTAAATATAC CAGATTTGGA TTTGAATGTC AATCCAAGAA TGAATCCTCC AACGGATACA 
CAAGCCAAAT TACTTAGGCA AATTCTACTT TCTGGTATGG CAAATCAAGT CGCACGCAAA 
GTTTCTCCAG ATGAAGTTGA GGAAGATCAC GACAAAGTAA AGTGGAAATA TGCATACAGA 
TGTTTGGAAA TGGAAGATCC AGTGTTCATG CACTCTTCGT CTGTTCTAAA AAAAACTTAC 
CCTGAATGGG TAGTTTATCA GGAGGTTTAT GAAACTAATA AAATATACAT GCGAGGCATT 
ACTGCCATTC AGCCAGAATG GTTACCAAAA TTTGCCACAT CCTTATGCCA ACTGAGCGAA 
CCTGTGAGCG ACCCGCCGCC TAGGTACGAT TCAAGAACGG GAAAGGTGAT GTGCCATGTA 
ACAGGGACAT TCGGTCGAGG TGGATGGCAA CTGCCGCTGA TTGAAGTAGA GTATCCAAAT 
TCCCTGGAAG GTATCAAGTG GTTTGCCCGA TTTTTTTTGG AAGGTCAAGT TTGCCCGGCA 
TTGGAACGAT TTGCTTCCTT GCTACTTTCT ACACCTGACA CAATGAACAA GACATGGGCA 
AAATTACTCC CGCGAACTGA AGCAATCACA AAAGCGCTAA TGGCTAATCA AGTTTCGTCA 
AAAGATCAAC TGTATCACAT TTGGAAAACG GATTCGAAAT TTCTTCTGAA TGCATACCAA 
AAGTGGTTGC CAGAATCGGC GCAGAATGAA GTTGCAATAC TTTGGCCACC GTTATAG