NECAME_02769 cDNA ORF clone, Necator americanus

Gene
Clones
gRNAs

Summary

Gene Symbol	NECAME_02769
Entrez Gene ID	25342807
Full Name	thrombospondin type 1 domain protein
Gene Type	protein-coding
Organism	Necator americanus

mRNA and Protein(s)

mRNA	Protein	Name
XM_013445678.1	XP_013301132.1	thrombospondin type 1 domain protein

Pathways from BioSystems
Gene Ontology

The following NECAME_02769 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NECAME_02769 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe129543	XM_013445678.1 Latest version!	Necator americanus thrombospondin type 1 domain protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NECAME_02769 ( XM_013445678.1 ) cDNA ORF clone, Necator americanus -> XP_013301132.1 Necator americanus thrombospondin type 1 domain protein mRNA.

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CloneID	ONe129543
Clone ID Related Accession (Same CDS sequence)	XM_013445678.1
Accession Version	XM_013445678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-11
Organism	Necator americanus
Product	thrombospondin type 1 domain protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_013560250). COMPLETENESS: incomplete on both ends.

ATGTTCGAAG TGTCGGACAG CTATGTGCTT GAGGAGAACG GCATACGAAT AGGAATGTTC 
TCGGTTGGAC CGCCGGATAT CGAGATCCGT GTGCCGCAAA CACACCACAT GCTCCACGAA 
TTAAACGTGC AAATCGTGCC GAAATTATGC TTAGCGGAGA AATTCACCGT GAATCTTCTC 
TATCGGCCGA TAGATGTTCT TCCTGACGAG AGGTGGATCG TTATTGCAAC ATCTTCCGTT 
GTCTTCCACA TGGAATCGGT TCAAATCACT TTTCCGTGTG AAAATTTTAC AAATGCTGGC 
CTGTACAGAG TTGATCTAGT CAATTCTCAA GGATATGAGA TCCAGCTTTT TTTTTCAGAA 
GAGATCGATA TACCTCGTGG ACAATCATCG TTGAGTATAG GATGTTCACA ATTCGATATA 
TTCTATGTGA AATATTGTTT CGAATTGGTT TCCGTGGATC TGAGGTCATC AGTTTTTCAT 
TTATGGAAAT CAACGTGTGT TAATACAGAA CCGGAGAAAG GAGCGGAGGG GATATTCAAA 
CCAAAAGATT CTGCTTATTC GTCTCGTCAA TCTGCGTTTA CCTCATCACT TGAATCCAAG 
TGGATCTGTA GCCGAGAAGA AGAATCTACC GGAAACATTC CGGATATATT TAGAATTTTA 
GGATTCATAG CATATGTGCT CCTGTCAATA AAAGCGACCT GGGAAAGTTG GTCCGCTTGG 
TCGGAATGTT CAGCCACTTG CGGTGAATCT ATTCAGAAAC GTTATCGTTT TTGCGAAAAT 
CCCGAGCCGA AACGAGGAGT GGATTGTGTC GGAGAAATGA TTGAGACTCG AACGTGCGTG 
GTACCGGATT GTTTAACACT CATCAAGTCT CATGGAATGT TAACGAATTG TTCTTGTGGA 
TGTGCGCTGA ACGGGACATC TGGGTCGTTC TTCGCTACCG TAGCGGATGC GGAGAAATGT 
GGAGGAAATC AAACATGGTA CATGTCGGCA CGTACAAATT CTGTAGTCTC GGATTTTAGT 
GTCGCAATTG ATCCGAACGC CGCAGGGAAA TTGTTTTTCT TTCTGAGAGC TCCATATGAA 
GAACTTGTCT GGTTTTCCGG ATCGAATCAG GAACAAGCAT TCACGTTACG ATTGGACAGA 
CCAATATTTG TCGTTCTTTG GTATAAGGGA AATAATTCCG CTATAAAAGG CAGAAAAGGA 
TTCACTATTT CATTCTCAAC AAGAGAAGTG ACAAATCAAT TTCCAACACG AAACGCTCCG 
TCATGTCAAC CTCTATGTCC AGAAACTGTG ATCATTGCTC TACTCTCACT TATATTCCTC 
TTTATTATTT GTTTTCCTCC TTTTCTATGT GCCAGTTTGA CACGACGATT ACGGACTCGA 
AACTGCCACG ATCGTCCGCT TCTTGATAAA ATATACGATA GTGAAATGAT TCGATCAGGA 
AATACGGAAT ATACACAGGC AAGTGGTGAA AAACCGGCAG CATTCACTGC TCAAAGAAGT 
ATTGGCATTC AACTCAGTGT ACAGAGCACT CCAAGACCTA CTGTAAACCT TAAAATTCTA 
TCTTTTACTG TTAGCTCTCA TCCAGATCTA ATCCAAAATT CGAGTTTAAC AACTCGGGTT 
TCGAAAATAT TTTTTCTTCC GTTTCCAAGT GGTCAAGGAT ATTTCAGATG TGCTCGTACA 
TGTGTACCAA GCGATTCACC ACAACCACAT CATGGTACAT CAATTTCAAC GACAGATGAA 
CTAGAATATG ATTATTACGA TGGGACAACG ATTCCTGGAT CACTGCTTGC ACCAGTTGAT 
GATCTTTTAA TGTGTGAAAT TGAAATCGAT CAGATCATAG CTCAATCATC GTTGTATTCA 
AAACCGGTCG AAACTCATGA TATGTACACA CAGGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013301132.1
CDS	1..1899
Translation	MFEVSDSYVLEENGIRIGMFSVGPPDIEIRVPQTHHMLHELNVQIVPKLCLAEKFTVNLLYRPIDVLPDERWIVIATSSVVFHMESVQITFPCENFTNAGLYRVDLVNSQGYEIQLFFSEEIDIPRGQSSLSIGCSQFDIFYVKYCFELVSVDLRSSVFHLWKSTCVNTEPEKGAEGIFKPKDSAYSSRQSAFTSSLESKWICSREEESTGNIPDIFRILGFIAYVLLSIKATWESWSAWSECSATCGESIQKRYRFCENPEPKRGVDCVGEMIETRTCVVPDCLTLIKSHGMLTNCSCGCALNGTSGSFFATVADAEKCGGNQTWYMSARTNSVVSDFSVAIDPNAAGKLFFFLRAPYEELVWFSGSNQEQAFTLRLDRPIFVVLWYKGNNSAIKGRKGFTISFSTREVTNQFPTRNAPSCQPLCPETVIIALLSLIFLFIICFPPFLCASLTRRLRTRNCHDRPLLDKIYDSEMIRSGNTEYTQASGEKPAAFTAQRSIGIQLSVQSTPRPTVNLKILSFTVSSHPDLIQNSSLTTRVSKIFFLPFPSGQGYFRCARTCVPSDSPQPHHGTSISTTDELEYDYYDGTTIPGSLLAPVDDLLMCEIEIDQIIAQSSLYSKPVETHDMYTQV

Target ORF information:

RefSeq Version	XM_013445678.1
Organism	Necator americanus
Definition	Necator americanus thrombospondin type 1 domain protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013445678.1

ORF Insert Sequence:

ATGTTCGAAG TGTCGGACAG CTATGTGCTT GAGGAGAACG GCATACGAAT AGGAATGTTC 
TCGGTTGGAC CGCCGGATAT CGAGATCCGT GTGCCGCAAA CACACCACAT GCTCCACGAA 
TTAAACGTGC AAATCGTGCC GAAATTATGC TTAGCGGAGA AATTCACCGT GAATCTTCTC 
TATCGGCCGA TAGATGTTCT TCCTGACGAG AGGTGGATCG TTATTGCAAC ATCTTCCGTT 
GTCTTCCACA TGGAATCGGT TCAAATCACT TTTCCGTGTG AAAATTTTAC AAATGCTGGC 
CTGTACAGAG TTGATCTAGT CAATTCTCAA GGATATGAGA TCCAGCTTTT TTTTTCAGAA 
GAGATCGATA TACCTCGTGG ACAATCATCG TTGAGTATAG GATGTTCACA ATTCGATATA 
TTCTATGTGA AATATTGTTT CGAATTGGTT TCCGTGGATC TGAGGTCATC AGTTTTTCAT 
TTATGGAAAT CAACGTGTGT TAATACAGAA CCGGAGAAAG GAGCGGAGGG GATATTCAAA 
CCAAAAGATT CTGCTTATTC GTCTCGTCAA TCTGCGTTTA CCTCATCACT TGAATCCAAG 
TGGATCTGTA GCCGAGAAGA AGAATCTACC GGAAACATTC CGGATATATT TAGAATTTTA 
GGATTCATAG CATATGTGCT CCTGTCAATA AAAGCGACCT GGGAAAGTTG GTCCGCTTGG 
TCGGAATGTT CAGCCACTTG CGGTGAATCT ATTCAGAAAC GTTATCGTTT TTGCGAAAAT 
CCCGAGCCGA AACGAGGAGT GGATTGTGTC GGAGAAATGA TTGAGACTCG AACGTGCGTG 
GTACCGGATT GTTTAACACT CATCAAGTCT CATGGAATGT TAACGAATTG TTCTTGTGGA 
TGTGCGCTGA ACGGGACATC TGGGTCGTTC TTCGCTACCG TAGCGGATGC GGAGAAATGT 
GGAGGAAATC AAACATGGTA CATGTCGGCA CGTACAAATT CTGTAGTCTC GGATTTTAGT 
GTCGCAATTG ATCCGAACGC CGCAGGGAAA TTGTTTTTCT TTCTGAGAGC TCCATATGAA 
GAACTTGTCT GGTTTTCCGG ATCGAATCAG GAACAAGCAT TCACGTTACG ATTGGACAGA 
CCAATATTTG TCGTTCTTTG GTATAAGGGA AATAATTCCG CTATAAAAGG CAGAAAAGGA 
TTCACTATTT CATTCTCAAC AAGAGAAGTG ACAAATCAAT TTCCAACACG AAACGCTCCG 
TCATGTCAAC CTCTATGTCC AGAAACTGTG ATCATTGCTC TACTCTCACT TATATTCCTC 
TTTATTATTT GTTTTCCTCC TTTTCTATGT GCCAGTTTGA CACGACGATT ACGGACTCGA 
AACTGCCACG ATCGTCCGCT TCTTGATAAA ATATACGATA GTGAAATGAT TCGATCAGGA 
AATACGGAAT ATACACAGGC AAGTGGTGAA AAACCGGCAG CATTCACTGC TCAAAGAAGT 
ATTGGCATTC AACTCAGTGT ACAGAGCACT CCAAGACCTA CTGTAAACCT TAAAATTCTA 
TCTTTTACTG TTAGCTCTCA TCCAGATCTA ATCCAAAATT CGAGTTTAAC AACTCGGGTT 
TCGAAAATAT TTTTTCTTCC GTTTCCAAGT GGTCAAGGAT ATTTCAGATG TGCTCGTACA 
TGTGTACCAA GCGATTCACC ACAACCACAT CATGGTACAT CAATTTCAAC GACAGATGAA 
CTAGAATATG ATTATTACGA TGGGACAACG ATTCCTGGAT CACTGCTTGC ACCAGTTGAT 
GATCTTTTAA TGTGTGAAAT TGAAATCGAT CAGATCATAG CTCAATCATC GTTGTATTCA 
AAACCGGTCG AAACTCATGA TATGTACACA CAGGTGTAG