Ccdc158 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccdc158
Entrez Gene ID	103742451
Full Name	coiled-coil domain containing 158
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029568533.1	XP_029424393.1	coiled-coil domain-containing protein 158
XM_008843504.1	XP_008841726.1	coiled-coil domain-containing protein 158

Pathways from BioSystems
Gene Ontology

The following Ccdc158 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccdc158 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa133224	XM_029568533.1 Latest version!	Nannospalax galili coiled-coil domain containing 158 (Ccdc158), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ONa25892	XM_008843504.1 Latest version!	Nannospalax galili coiled-coil domain containing 158 (Ccdc158), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccdc158 ( XM_029568533.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029424393.1 Nannospalax galili coiled-coil domain containing 158 (Ccdc158), mRNA.

Price & Availability↑

CloneID	ONa133224
Clone ID Related Accession (Same CDS sequence)	XM_029568533.1
Accession Version	XM_029568533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	coiled-coil domain-containing protein 158
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008347125.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAA AAGCTTGTGA ATCAAATAGT GAAGATCTTT TATCAAGTGG CATCACAGCC 
AATGGAGGTT CTTCAAACCC ATTTTTTTTG TCATCTATTC GTGGTACAAT AATTGAAAAC 
ACATCTTCAG CTGGGACTTT GACGCAAATT CCTTTTTTCC CTAAATATGA AGTAGAACTT 
GATTCTCATA GAAAAGTCAT CCCTTACCCT GGAAAAGAGC ACATAGAATG TGTTTTGGAG 
AAATACTCAC ATCAGGTCAA AGATTTACAG AGAAGACTAA ATGAAAGCGA TGAATTACAT 
GAGAAACAAA AGTTTTACTT AAGGCAGTCA GTCATTGATT TGCAAACAAA GCTTCAAGAA 
ATGCAAATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAATCA ATTCCAGGAG 
GATTTAAGAA ATCAACTTCA AAATACAGTT CATGAACTTG AAGCCGCCAA AAGCCTTAAG 
GAGGGCATGT TAAAAGACAG CAGCACACAG ATAGAGCAGC TAAGAAAAAT GATGATTAGT 
TATGAAGGAG TGCTTCAAGA AATCCGATCA ATCTTAGTTG ACTTTGAAGA AGCCTCAGGC 
AAGAAAATCT GTGAACAGGA CAACATGTCT ACTATGCACT TCCGCAGCCT GGGCTCAACT 
ATTAGTAAAA TCCTAAGAGA ATTAGACACA GAAATTTCTT ATCTTAAAGG GAGGATATTT 
CCAGTAGAGG ATCAGCTTGA AACACTGAAA TCTGAATCAC AGAACAAAAT AGAACTATTA 
CTTCAACAAC ATCAAGATAG GATTGAGCAG TTGATAAGTG AACATGAAGT TGAAACAACA 
GGACTCACTG AGAAAGCCAA TAGTGCTCGA AGCCAAGCCA ATAGCATCCA GAGTCAATTG 
GAAATCATTC AAGAACAAGC AAGAAACCAA AACTCAATGT ATATGCGTCA GCTCAGTGAC 
TTGGAATCTA CCGTCTCTCA GCTGCGTTCG GAATTGAGGG AGGCCAAACG AATGTATGAA 
GATAAGATAG AAGAGCTGGA GAAGCAGTTA GTCCTTGCCA ATTCAGAGCT CACGGAGGCA 
TGCACAGAAC GGGATCAGTT CAGTCAGGAA TCTGGAAGTT TGGATGATCA ACTTCAAAAG 
CTGTTGGCAG ATCTACACAA AAGGGAGAAA GAACTGAGCC TGGAGAAGGA GCAGAATAAG 
CGTCTATGGG ACCGAGATAC AGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAGCTA 
GATGACAGGA ATATGGAGGT GCAGCGGCTA GAGGCCCTCC TCAAGGCCAT GAAGAGCGAG 
TGTCAAGGCC AGATGGAACG GCAGATGGCA GCAATTCAGG GAAAGAACGA GAGCCTGGAG 
AAAGTGTCTT CCCTGACTGC ACAGCTTGAA TCCACCAAGG AGATGCTGCA CAAGGTGTTG 
GAAGAGTTAA CGGCCAAGAA GATGATGCTT GAGAGCTCAG AGAGGACGGT GTCTGACCTG 
ACAGCTTCCC TCCAGGAAAA GGAGGGAGCT ATTGAGGCCA CCAATGCAGA GATCACCAAA 
CTCCGCTCCC GGGTAGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT 
CACCTCAGAA ATGTGCAAAT GGAATGTGAG GCGCTCAAGC TCCAGGTGGC AGAAAAAGAC 
AAAGTGATTG AGATTTTGCG CCAGCAGATT GAAAACATGA CGCAGCTGGT TGGCCAGCAT 
GGACGAACGG CCGGTGCTCT GCAAGCAGAA AAGGCGCAAC TGGAGAAGGA GATTCATGAT 
CGCAAACTGG AATTGCAGGA ATTTAAGATT CTGAAAGATA AAAAAGATGC AAAGATTCGG 
GAACTTGAAG CCAGAGTGAG TGACCTAGAA CTGGAAAAGG TGAAGCTGGT GAATGCAGGC 
TCTGAGTGGC TCCGTGCAAT GAAGGATATC AAACAAGAGA GAGATCAGTT GTTAAATGAG 
GTGAAGAGTA ATAGGAATGA ATTAAATAAT CTTTCAGAGG ACTATGAAGT CTTAAAAAGG 
AATTTTCAAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAACTGAA AATGCAATTA 
AAATCTGCAC AGTCTGAACT GGAGCAAACC AGGAATACAC TAAAGACCAT GGAAGGATCT 
GATGGCCATG CTATGAAGGT GGCAATGGGG ATGCAAAAGC AAATCTCAGC CAAAAGAGGT 
CAGATAGATG CCTTGCAGAG CAAGATCCAG TTTTTGGAAG AGGCTATGAC AAATGCAAAC 
AAGGAGAAAC ATTTTCTGAA AGAAGAAAAG AGTAAACTGA GCCAGGAATT GAGTACTGTC 
ACAACAGAAA AAAACAAGAT GGCTGGGGAA CTGGGAGTGT TGCGATCTCA GGAGCGCTGC 
TTGAAAGAAA GGGTTGCCAG TATGGAAGCT GCTCTTGATA AGGCATCTTT GCAGTTTGCA 
GAATGTCAAG ATATAATACA GCGCCAGGAA CAAGAATCTG TGAGCCTAAA GCTCCAACAC 
ACTTTGGATG TAAAAGAACT TCAGGGCCCC GGATACACCT CCAATTCTTC AATGAAGCCA 
CACCTTCTCC AGCCAGCATC TGTAACTCAT TCTCATTCTA ATGTACCATC TTCCCAGTCT 
ACAGCCAGTT TCCTGTCTCA TCACTCTATG AAAACCAACA CTTTGAAGGA AGACACAACA 
ACGGACTTGA AACAGCTTCT CCAAAAGTTG CGAAGTGTGA TCAACGAGGA GCCTGACATG 
CCTCTGAGCA AGACCAACGA GAATGGGAGG ACACAGTCCC CCACAGTCTT AGATGATAGA 
GTAAGAGACT GCATTATTGA ATCAAGCCTG AGAGCAGACC TCTGCCACAG AGAACCAGTC 
ACATTGCATC CAGGAGACCT GGAAGATCCA TGTAGTTGCT TCACCTTCCC ATCTGCAGTA 
AGTCCATCTA TGAAAAATTC ATCTTCTCGG TCATTCAATT CTCCTAAGTC CCCAGTGCAC 
TCACTCCTGA CTAGTTCTAC TGAAGAATCA GTAGGCTCCA CATCACAGGA CAGATCTACC 
GAACCTACTC ATTCACCTGA CGCTGCAAAA GATTCACAGT CTCCGTCACT AGAAACAACA 
GGAAAAACAT GCCAAAAACT TCAGAACAGA CTGGAAAGCT TGCAAACCCT GGTAGAAGAT 
TTACAGCTGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ACCAAGAAAA GAGGATTCAG 
AAAGTGAAAG ATCAGGAAAA AATGTTATTA AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029424393.1
CDS	286..3561
Translation	MESKACESNSEDLLSSGITANGGSSNPFFLSSIRGTIIENTSSAGTLTQIPFFPKYEVELDSHRKVIPYPGKEHIECVLEKYSHQVKDLQRRLNESDELHEKQKFYLRQSVIDLQTKLQEMQMERDAMADIRRRENQFQEDLRNQLQNTVHELEAAKSLKEGMLKDSSTQIEQLRKMMISYEGVLQEIRSILVDFEEASGKKICEQDNMSTMHFRSLGSTISKILRELDTEISYLKGRIFPVEDQLETLKSESQNKIELLLQQHQDRIEQLISEHEVETTGLTEKANSARSQANSIQSQLEIIQEQARNQNSMYMRQLSDLESTVSQLRSELREAKRMYEDKIEELEKQLVLANSELTEACTERDQFSQESGSLDDQLQKLLADLHKREKELSLEKEQNKRLWDRDTGNSITIDHLRRELDDRNMEVQRLEALLKAMKSECQGQMERQMAAIQGKNESLEKVSSLTAQLESTKEMLHKVLEELTAKKMMLESSERTVSDLTASLQEKEGAIEATNAEITKLRSRVDLKLQELQHLKNEGDHLRNVQMECEALKLQVAEKDKVIEILRQQIENMTQLVGQHGRTAGALQAEKAQLEKEIHDRKLELQEFKILKDKKDAKIRELEARVSDLELEKVKLVNAGSEWLRAMKDIKQERDQLLNEVKSNRNELNNLSEDYEVLKRNFQNKSEEMETTTNKLKMQLKSAQSELEQTRNTLKTMEGSDGHAMKVAMGMQKQISAKRGQIDALQSKIQFLEEAMTNANKEKHFLKEEKSKLSQELSTVTTEKNKMAGELGVLRSQERCLKERVASMEAALDKASLQFAECQDIIQRQEQESVSLKLQHTLDVKELQGPGYTSNSSMKPHLLQPASVTHSHSNVPSSQSTASFLSHHSMKTNTLKEDTTTDLKQLLQKLRSVINEEPDMPLSKTNENGRTQSPTVLDDRVRDCIIESSLRADLCHREPVTLHPGDLEDPCSCFTFPSAVSPSMKNSSSRSFNSPKSPVHSLLTSSTEESVGSTSQDRSTEPTHSPDAAKDSQSPSLETTGKTCQKLQNRLESLQTLVEDLQLKNQAMSSMIRNQEKRIQKVKDQEKMLLK

Target ORF information:

RefSeq Version	XM_029568533.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili coiled-coil domain containing 158 (Ccdc158), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029568533.1

ORF Insert Sequence:

ATGGAATCAA AAGCTTGTGA ATCAAATAGT GAAGATCTTT TATCAAGTGG CATCACAGCC 
AATGGAGGTT CTTCAAACCC ATTTTTTTTG TCATCTATTC GTGGTACAAT AATTGAAAAC 
ACATCTTCAG CTGGGACTTT GACGCAAATT CCTTTTTTCC CTAAATATGA AGTAGAACTT 
GATTCTCATA GAAAAGTCAT CCCTTACCCT GGAAAAGAGC ACATAGAATG TGTTTTGGAG 
AAATACTCAC ATCAGGTCAA AGATTTACAG AGAAGACTAA ATGAAAGCGA TGAATTACAT 
GAGAAACAAA AGTTTTACTT AAGGCAGTCA GTCATTGATT TGCAAACAAA GCTTCAAGAA 
ATGCAAATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAATCA ATTCCAGGAG 
GATTTAAGAA ATCAACTTCA AAATACAGTT CATGAACTTG AAGCCGCCAA AAGCCTTAAG 
GAGGGCATGT TAAAAGACAG CAGCACACAG ATAGAGCAGC TAAGAAAAAT GATGATTAGT 
TATGAAGGAG TGCTTCAAGA AATCCGATCA ATCTTAGTTG ACTTTGAAGA AGCCTCAGGC 
AAGAAAATCT GTGAACAGGA CAACATGTCT ACTATGCACT TCCGCAGCCT GGGCTCAACT 
ATTAGTAAAA TCCTAAGAGA ATTAGACACA GAAATTTCTT ATCTTAAAGG GAGGATATTT 
CCAGTAGAGG ATCAGCTTGA AACACTGAAA TCTGAATCAC AGAACAAAAT AGAACTATTA 
CTTCAACAAC ATCAAGATAG GATTGAGCAG TTGATAAGTG AACATGAAGT TGAAACAACA 
GGACTCACTG AGAAAGCCAA TAGTGCTCGA AGCCAAGCCA ATAGCATCCA GAGTCAATTG 
GAAATCATTC AAGAACAAGC AAGAAACCAA AACTCAATGT ATATGCGTCA GCTCAGTGAC 
TTGGAATCTA CCGTCTCTCA GCTGCGTTCG GAATTGAGGG AGGCCAAACG AATGTATGAA 
GATAAGATAG AAGAGCTGGA GAAGCAGTTA GTCCTTGCCA ATTCAGAGCT CACGGAGGCA 
TGCACAGAAC GGGATCAGTT CAGTCAGGAA TCTGGAAGTT TGGATGATCA ACTTCAAAAG 
CTGTTGGCAG ATCTACACAA AAGGGAGAAA GAACTGAGCC TGGAGAAGGA GCAGAATAAG 
CGTCTATGGG ACCGAGATAC AGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAGCTA 
GATGACAGGA ATATGGAGGT GCAGCGGCTA GAGGCCCTCC TCAAGGCCAT GAAGAGCGAG 
TGTCAAGGCC AGATGGAACG GCAGATGGCA GCAATTCAGG GAAAGAACGA GAGCCTGGAG 
AAAGTGTCTT CCCTGACTGC ACAGCTTGAA TCCACCAAGG AGATGCTGCA CAAGGTGTTG 
GAAGAGTTAA CGGCCAAGAA GATGATGCTT GAGAGCTCAG AGAGGACGGT GTCTGACCTG 
ACAGCTTCCC TCCAGGAAAA GGAGGGAGCT ATTGAGGCCA CCAATGCAGA GATCACCAAA 
CTCCGCTCCC GGGTAGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT 
CACCTCAGAA ATGTGCAAAT GGAATGTGAG GCGCTCAAGC TCCAGGTGGC AGAAAAAGAC 
AAAGTGATTG AGATTTTGCG CCAGCAGATT GAAAACATGA CGCAGCTGGT TGGCCAGCAT 
GGACGAACGG CCGGTGCTCT GCAAGCAGAA AAGGCGCAAC TGGAGAAGGA GATTCATGAT 
CGCAAACTGG AATTGCAGGA ATTTAAGATT CTGAAAGATA AAAAAGATGC AAAGATTCGG 
GAACTTGAAG CCAGAGTGAG TGACCTAGAA CTGGAAAAGG TGAAGCTGGT GAATGCAGGC 
TCTGAGTGGC TCCGTGCAAT GAAGGATATC AAACAAGAGA GAGATCAGTT GTTAAATGAG 
GTGAAGAGTA ATAGGAATGA ATTAAATAAT CTTTCAGAGG ACTATGAAGT CTTAAAAAGG 
AATTTTCAAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAACTGAA AATGCAATTA 
AAATCTGCAC AGTCTGAACT GGAGCAAACC AGGAATACAC TAAAGACCAT GGAAGGATCT 
GATGGCCATG CTATGAAGGT GGCAATGGGG ATGCAAAAGC AAATCTCAGC CAAAAGAGGT 
CAGATAGATG CCTTGCAGAG CAAGATCCAG TTTTTGGAAG AGGCTATGAC AAATGCAAAC 
AAGGAGAAAC ATTTTCTGAA AGAAGAAAAG AGTAAACTGA GCCAGGAATT GAGTACTGTC 
ACAACAGAAA AAAACAAGAT GGCTGGGGAA CTGGGAGTGT TGCGATCTCA GGAGCGCTGC 
TTGAAAGAAA GGGTTGCCAG TATGGAAGCT GCTCTTGATA AGGCATCTTT GCAGTTTGCA 
GAATGTCAAG ATATAATACA GCGCCAGGAA CAAGAATCTG TGAGCCTAAA GCTCCAACAC 
ACTTTGGATG TAAAAGAACT TCAGGGCCCC GGATACACCT CCAATTCTTC AATGAAGCCA 
CACCTTCTCC AGCCAGCATC TGTAACTCAT TCTCATTCTA ATGTACCATC TTCCCAGTCT 
ACAGCCAGTT TCCTGTCTCA TCACTCTATG AAAACCAACA CTTTGAAGGA AGACACAACA 
ACGGACTTGA AACAGCTTCT CCAAAAGTTG CGAAGTGTGA TCAACGAGGA GCCTGACATG 
CCTCTGAGCA AGACCAACGA GAATGGGAGG ACACAGTCCC CCACAGTCTT AGATGATAGA 
GTAAGAGACT GCATTATTGA ATCAAGCCTG AGAGCAGACC TCTGCCACAG AGAACCAGTC 
ACATTGCATC CAGGAGACCT GGAAGATCCA TGTAGTTGCT TCACCTTCCC ATCTGCAGTA 
AGTCCATCTA TGAAAAATTC ATCTTCTCGG TCATTCAATT CTCCTAAGTC CCCAGTGCAC 
TCACTCCTGA CTAGTTCTAC TGAAGAATCA GTAGGCTCCA CATCACAGGA CAGATCTACC 
GAACCTACTC ATTCACCTGA CGCTGCAAAA GATTCACAGT CTCCGTCACT AGAAACAACA 
GGAAAAACAT GCCAAAAACT TCAGAACAGA CTGGAAAGCT TGCAAACCCT GGTAGAAGAT 
TTACAGCTGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ACCAAGAAAA GAGGATTCAG 
AAAGTGAAAG ATCAGGAAAA AATGTTATTA AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccdc158 ( XM_008843504.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008841726.1 Nannospalax galili coiled-coil domain containing 158 (Ccdc158), mRNA.

Price & Availability↑

CloneID	ONa25892
Clone ID Related Accession (Same CDS sequence)	XM_008843504.1
Accession Version	XM_008843504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	coiled-coil domain-containing protein 158
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008347125.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAA AAGCTTGTGA ATCAAATAGT GAAGATCTTT TATCAAGTGG CATCACAGCC 
AATGGAGGTT CTTCAAACCC ATTTTTTTTG TCATCTATTC GTGGTACAAT AATTGAAAAC 
ACATCTTCAG CTGGGACTTT GACGCAAATT CCTTTTTTCC CTAAATATGA AGTAGAACTT 
GATTCTCATA GAAAAGTCAT CCCTTACCCT GGAAAAGAGC ACATAGAATG TGTTTTGGAG 
AAATACTCAC ATCAGGTCAA AGATTTACAG AGAAGACTAA ATGAAAGCGA TGAATTACAT 
GAGAAACAAA AGTTTTACTT AAGGCAGTCA GTCATTGATT TGCAAACAAA GCTTCAAGAA 
ATGCAAATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAATCA ATTCCAGGAG 
GATTTAAGAA ATCAACTTCA AAATACAGTT CATGAACTTG AAGCCGCCAA AAGCCTTAAG 
GAGGGCATGT TAAAAGACAG CAGCACACAG ATAGAGCAGC TAAGAAAAAT GATGATTAGT 
TATGAAGGAG TGCTTCAAGA AATCCGATCA ATCTTAGTTG ACTTTGAAGA AGCCTCAGGC 
AAGAAAATCT GTGAACAGGA CAACATGTCT ACTATGCACT TCCGCAGCCT GGGCTCAACT 
ATTAGTAAAA TCCTAAGAGA ATTAGACACA GAAATTTCTT ATCTTAAAGG GAGGATATTT 
CCAGTAGAGG ATCAGCTTGA AACACTGAAA TCTGAATCAC AGAACAAAAT AGAACTATTA 
CTTCAACAAC ATCAAGATAG GATTGAGCAG TTGATAAGTG AACATGAAGT TGAAACAACA 
GGACTCACTG AGAAAGCCAA TAGTGCTCGA AGCCAAGCCA ATAGCATCCA GAGTCAATTG 
GAAATCATTC AAGAACAAGC AAGAAACCAA AACTCAATGT ATATGCGTCA GCTCAGTGAC 
TTGGAATCTA CCGTCTCTCA GCTGCGTTCG GAATTGAGGG AGGCCAAACG AATGTATGAA 
GATAAGATAG AAGAGCTGGA GAAGCAGTTA GTCCTTGCCA ATTCAGAGCT CACGGAGGCA 
TGCACAGAAC GGGATCAGTT CAGTCAGGAA TCTGGAAGTT TGGATGATCA ACTTCAAAAG 
CTGTTGGCAG ATCTACACAA AAGGGAGAAA GAACTGAGCC TGGAGAAGGA GCAGAATAAG 
CGTCTATGGG ACCGAGATAC AGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAGCTA 
GATGACAGGA ATATGGAGGT GCAGCGGCTA GAGGCCCTCC TCAAGGCCAT GAAGAGCGAG 
TGTCAAGGCC AGATGGAACG GCAGATGGCA GCAATTCAGG GAAAGAACGA GAGCCTGGAG 
AAAGTGTCTT CCCTGACTGC ACAGCTTGAA TCCACCAAGG AGATGCTGCA CAAGGTGTTG 
GAAGAGTTAA CGGCCAAGAA GATGATGCTT GAGAGCTCAG AGAGGACGGT GTCTGACCTG 
ACAGCTTCCC TCCAGGAAAA GGAGGGAGCT ATTGAGGCCA CCAATGCAGA GATCACCAAA 
CTCCGCTCCC GGGTAGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT 
CACCTCAGAA ATGTGCAAAT GGAATGTGAG GCGCTCAAGC TCCAGGTGGC AGAAAAAGAC 
AAAGTGATTG AGATTTTGCG CCAGCAGATT GAAAACATGA CGCAGCTGGT TGGCCAGCAT 
GGACGAACGG CCGGTGCTCT GCAAGCAGAA AAGGCGCAAC TGGAGAAGGA GATTCATGAT 
CGCAAACTGG AATTGCAGGA ATTTAAGATT CTGAAAGATA AAAAAGATGC AAAGATTCGG 
GAACTTGAAG CCAGAGTGAG TGACCTAGAA CTGGAAAAGG TGAAGCTGGT GAATGCAGGC 
TCTGAGTGGC TCCGTGCAAT GAAGGATATC AAACAAGAGA GAGATCAGTT GTTAAATGAG 
GTGAAGAGTA ATAGGAATGA ATTAAATAAT CTTTCAGAGG ACTATGAAGT CTTAAAAAGG 
AATTTTCAAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAACTGAA AATGCAATTA 
AAATCTGCAC AGTCTGAACT GGAGCAAACC AGGAATACAC TAAAGACCAT GGAAGGATCT 
GATGGCCATG CTATGAAGGT GGCAATGGGG ATGCAAAAGC AAATCTCAGC CAAAAGAGGT 
CAGATAGATG CCTTGCAGAG CAAGATCCAG TTTTTGGAAG AGGCTATGAC AAATGCAAAC 
AAGGAGAAAC ATTTTCTGAA AGAAGAAAAG AGTAAACTGA GCCAGGAATT GAGTACTGTC 
ACAACAGAAA AAAACAAGAT GGCTGGGGAA CTGGGAGTGT TGCGATCTCA GGAGCGCTGC 
TTGAAAGAAA GGGTTGCCAG TATGGAAGCT GCTCTTGATA AGGCATCTTT GCAGTTTGCA 
GAATGTCAAG ATATAATACA GCGCCAGGAA CAAGAATCTG TGAGCCTAAA GCTCCAACAC 
ACTTTGGATG TAAAAGAACT TCAGGGCCCC GGATACACCT CCAATTCTTC AATGAAGCCA 
CACCTTCTCC AGCCAGCATC TGTAACTCAT TCTCATTCTA ATGTACCATC TTCCCAGTCT 
ACAGCCAGTT TCCTGTCTCA TCACTCTATG AAAACCAACA CTTTGAAGGA AGACACAACA 
ACGGACTTGA AACAGCTTCT CCAAAAGTTG CGAAGTGTGA TCAACGAGGA GCCTGACATG 
CCTCTGAGCA AGACCAACGA GAATGGGAGG ACACAGTCCC CCACAGTCTT AGATGATAGA 
GTAAGAGACT GCATTATTGA ATCAAGCCTG AGAGCAGACC TCTGCCACAG AAGCAGCAGC 
TCATTAAGGG AGTCCACTGA AGGTAGCAAG TCAGGTGAAA TGCTTAGCAG AGAACCAGTC 
ACATTGCATC CAGGAGACCT GGAAGATCCA TGTAGTTGCT TCACCTTCCC ATCTGCAGTA 
AGTCCATCTA TGAAAAATTC ATCTTCTCGG TCATTCAATT CTCCTAAGTC CCCAGTGCAC 
TCACTCCTGA CTAGTTCTAC TGAAGAATCA GTAGGCTCCA CATCACAGGA CAGATCTACC 
GAACCTACTC ATTCACCTGA CGCTGCAAAA GATTCACAGT CTCCGTCACT AGAAACAACA 
GGAAAAACAT GCCAAAAACT TCAGAACAGA CTGGAAAGCT TGCAAACCCT GGTAGAAGAT 
TTACAGCTGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ACCAAGAAAA GAGGATTCAG 
AAAGTGAAAG ATCAGGAAAA AATGTTATTA AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008841726.1
CDS	1..3336
Translation	MESKACESNSEDLLSSGITANGGSSNPFFLSSIRGTIIENTSSAGTLTQIPFFPKYEVELDSHRKVIPYPGKEHIECVLEKYSHQVKDLQRRLNESDELHEKQKFYLRQSVIDLQTKLQEMQMERDAMADIRRRENQFQEDLRNQLQNTVHELEAAKSLKEGMLKDSSTQIEQLRKMMISYEGVLQEIRSILVDFEEASGKKICEQDNMSTMHFRSLGSTISKILRELDTEISYLKGRIFPVEDQLETLKSESQNKIELLLQQHQDRIEQLISEHEVETTGLTEKANSARSQANSIQSQLEIIQEQARNQNSMYMRQLSDLESTVSQLRSELREAKRMYEDKIEELEKQLVLANSELTEACTERDQFSQESGSLDDQLQKLLADLHKREKELSLEKEQNKRLWDRDTGNSITIDHLRRELDDRNMEVQRLEALLKAMKSECQGQMERQMAAIQGKNESLEKVSSLTAQLESTKEMLHKVLEELTAKKMMLESSERTVSDLTASLQEKEGAIEATNAEITKLRSRVDLKLQELQHLKNEGDHLRNVQMECEALKLQVAEKDKVIEILRQQIENMTQLVGQHGRTAGALQAEKAQLEKEIHDRKLELQEFKILKDKKDAKIRELEARVSDLELEKVKLVNAGSEWLRAMKDIKQERDQLLNEVKSNRNELNNLSEDYEVLKRNFQNKSEEMETTTNKLKMQLKSAQSELEQTRNTLKTMEGSDGHAMKVAMGMQKQISAKRGQIDALQSKIQFLEEAMTNANKEKHFLKEEKSKLSQELSTVTTEKNKMAGELGVLRSQERCLKERVASMEAALDKASLQFAECQDIIQRQEQESVSLKLQHTLDVKELQGPGYTSNSSMKPHLLQPASVTHSHSNVPSSQSTASFLSHHSMKTNTLKEDTTTDLKQLLQKLRSVINEEPDMPLSKTNENGRTQSPTVLDDRVRDCIIESSLRADLCHRSSSSLRESTEGSKSGEMLSREPVTLHPGDLEDPCSCFTFPSAVSPSMKNSSSRSFNSPKSPVHSLLTSSTEESVGSTSQDRSTEPTHSPDAAKDSQSPSLETTGKTCQKLQNRLESLQTLVEDLQLKNQAMSSMIRNQEKRIQKVKDQEKMLLK

Target ORF information:

RefSeq Version	XM_008843504.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili coiled-coil domain containing 158 (Ccdc158), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008843504.1

ORF Insert Sequence:

ATGGAATCAA AAGCTTGTGA ATCAAATAGT GAAGATCTTT TATCAAGTGG CATCACAGCC 
AATGGAGGTT CTTCAAACCC ATTTTTTTTG TCATCTATTC GTGGTACAAT AATTGAAAAC 
ACATCTTCAG CTGGGACTTT GACGCAAATT CCTTTTTTCC CTAAATATGA AGTAGAACTT 
GATTCTCATA GAAAAGTCAT CCCTTACCCT GGAAAAGAGC ACATAGAATG TGTTTTGGAG 
AAATACTCAC ATCAGGTCAA AGATTTACAG AGAAGACTAA ATGAAAGCGA TGAATTACAT 
GAGAAACAAA AGTTTTACTT AAGGCAGTCA GTCATTGATT TGCAAACAAA GCTTCAAGAA 
ATGCAAATGG AGAGAGATGC CATGGCTGAC ATCAGACGAA GGGAGAATCA ATTCCAGGAG 
GATTTAAGAA ATCAACTTCA AAATACAGTT CATGAACTTG AAGCCGCCAA AAGCCTTAAG 
GAGGGCATGT TAAAAGACAG CAGCACACAG ATAGAGCAGC TAAGAAAAAT GATGATTAGT 
TATGAAGGAG TGCTTCAAGA AATCCGATCA ATCTTAGTTG ACTTTGAAGA AGCCTCAGGC 
AAGAAAATCT GTGAACAGGA CAACATGTCT ACTATGCACT TCCGCAGCCT GGGCTCAACT 
ATTAGTAAAA TCCTAAGAGA ATTAGACACA GAAATTTCTT ATCTTAAAGG GAGGATATTT 
CCAGTAGAGG ATCAGCTTGA AACACTGAAA TCTGAATCAC AGAACAAAAT AGAACTATTA 
CTTCAACAAC ATCAAGATAG GATTGAGCAG TTGATAAGTG AACATGAAGT TGAAACAACA 
GGACTCACTG AGAAAGCCAA TAGTGCTCGA AGCCAAGCCA ATAGCATCCA GAGTCAATTG 
GAAATCATTC AAGAACAAGC AAGAAACCAA AACTCAATGT ATATGCGTCA GCTCAGTGAC 
TTGGAATCTA CCGTCTCTCA GCTGCGTTCG GAATTGAGGG AGGCCAAACG AATGTATGAA 
GATAAGATAG AAGAGCTGGA GAAGCAGTTA GTCCTTGCCA ATTCAGAGCT CACGGAGGCA 
TGCACAGAAC GGGATCAGTT CAGTCAGGAA TCTGGAAGTT TGGATGATCA ACTTCAAAAG 
CTGTTGGCAG ATCTACACAA AAGGGAGAAA GAACTGAGCC TGGAGAAGGA GCAGAATAAG 
CGTCTATGGG ACCGAGATAC AGGCAACAGC ATCACCATCG ACCACCTGCG GCGGGAGCTA 
GATGACAGGA ATATGGAGGT GCAGCGGCTA GAGGCCCTCC TCAAGGCCAT GAAGAGCGAG 
TGTCAAGGCC AGATGGAACG GCAGATGGCA GCAATTCAGG GAAAGAACGA GAGCCTGGAG 
AAAGTGTCTT CCCTGACTGC ACAGCTTGAA TCCACCAAGG AGATGCTGCA CAAGGTGTTG 
GAAGAGTTAA CGGCCAAGAA GATGATGCTT GAGAGCTCAG AGAGGACGGT GTCTGACCTG 
ACAGCTTCCC TCCAGGAAAA GGAGGGAGCT ATTGAGGCCA CCAATGCAGA GATCACCAAA 
CTCCGCTCCC GGGTAGACTT GAAGTTGCAG GAGCTGCAGC ACCTGAAGAA TGAAGGGGAT 
CACCTCAGAA ATGTGCAAAT GGAATGTGAG GCGCTCAAGC TCCAGGTGGC AGAAAAAGAC 
AAAGTGATTG AGATTTTGCG CCAGCAGATT GAAAACATGA CGCAGCTGGT TGGCCAGCAT 
GGACGAACGG CCGGTGCTCT GCAAGCAGAA AAGGCGCAAC TGGAGAAGGA GATTCATGAT 
CGCAAACTGG AATTGCAGGA ATTTAAGATT CTGAAAGATA AAAAAGATGC AAAGATTCGG 
GAACTTGAAG CCAGAGTGAG TGACCTAGAA CTGGAAAAGG TGAAGCTGGT GAATGCAGGC 
TCTGAGTGGC TCCGTGCAAT GAAGGATATC AAACAAGAGA GAGATCAGTT GTTAAATGAG 
GTGAAGAGTA ATAGGAATGA ATTAAATAAT CTTTCAGAGG ACTATGAAGT CTTAAAAAGG 
AATTTTCAAA ACAAAAGTGA AGAAATGGAA ACTACTACAA ATAAACTGAA AATGCAATTA 
AAATCTGCAC AGTCTGAACT GGAGCAAACC AGGAATACAC TAAAGACCAT GGAAGGATCT 
GATGGCCATG CTATGAAGGT GGCAATGGGG ATGCAAAAGC AAATCTCAGC CAAAAGAGGT 
CAGATAGATG CCTTGCAGAG CAAGATCCAG TTTTTGGAAG AGGCTATGAC AAATGCAAAC 
AAGGAGAAAC ATTTTCTGAA AGAAGAAAAG AGTAAACTGA GCCAGGAATT GAGTACTGTC 
ACAACAGAAA AAAACAAGAT GGCTGGGGAA CTGGGAGTGT TGCGATCTCA GGAGCGCTGC 
TTGAAAGAAA GGGTTGCCAG TATGGAAGCT GCTCTTGATA AGGCATCTTT GCAGTTTGCA 
GAATGTCAAG ATATAATACA GCGCCAGGAA CAAGAATCTG TGAGCCTAAA GCTCCAACAC 
ACTTTGGATG TAAAAGAACT TCAGGGCCCC GGATACACCT CCAATTCTTC AATGAAGCCA 
CACCTTCTCC AGCCAGCATC TGTAACTCAT TCTCATTCTA ATGTACCATC TTCCCAGTCT 
ACAGCCAGTT TCCTGTCTCA TCACTCTATG AAAACCAACA CTTTGAAGGA AGACACAACA 
ACGGACTTGA AACAGCTTCT CCAAAAGTTG CGAAGTGTGA TCAACGAGGA GCCTGACATG 
CCTCTGAGCA AGACCAACGA GAATGGGAGG ACACAGTCCC CCACAGTCTT AGATGATAGA 
GTAAGAGACT GCATTATTGA ATCAAGCCTG AGAGCAGACC TCTGCCACAG AAGCAGCAGC 
TCATTAAGGG AGTCCACTGA AGGTAGCAAG TCAGGTGAAA TGCTTAGCAG AGAACCAGTC 
ACATTGCATC CAGGAGACCT GGAAGATCCA TGTAGTTGCT TCACCTTCCC ATCTGCAGTA 
AGTCCATCTA TGAAAAATTC ATCTTCTCGG TCATTCAATT CTCCTAAGTC CCCAGTGCAC 
TCACTCCTGA CTAGTTCTAC TGAAGAATCA GTAGGCTCCA CATCACAGGA CAGATCTACC 
GAACCTACTC ATTCACCTGA CGCTGCAAAA GATTCACAGT CTCCGTCACT AGAAACAACA 
GGAAAAACAT GCCAAAAACT TCAGAACAGA CTGGAAAGCT TGCAAACCCT GGTAGAAGAT 
TTACAGCTGA AGAACCAAGC AATGTCTTCA ATGATCAGAA ACCAAGAAAA GAGGATTCAG 
AAAGTGAAAG ATCAGGAAAA AATGTTATTA AAATGA