Dagla cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dagla
Entrez Gene ID	103729120
Full Name	diacylglycerol lipase alpha
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008827106.1	XP_008825328.1	sn1-specific diacylglycerol lipase alpha
XM_008827106.2	XP_008825328.1	sn1-specific diacylglycerol lipase alpha

Pathways from BioSystems
Gene Ontology

The following Dagla gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dagla cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONa44147	XM_008827106.2 Latest version!	Nannospalax galili diacylglycerol lipase alpha (Dagla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
ONa44147	XM_008827106.1	Nannospalax galili diacylglycerol lipase alpha (Dagla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dagla ( XM_008827106.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008825328.1 Nannospalax galili diacylglycerol lipase alpha (Dagla), mRNA.

Price & Availability↑

CloneID	ONa44147
Clone ID Related Accession (Same CDS sequence)	XM_008827106.1 , XM_008827106.2
Accession Version	XM_008827106.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008331995.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TTGTGGTGTT CCGCCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCTTG 
CCAGCCATCT TTCTCTTCCT CCTGCACACC ACCTGGTTTG TGATCCTGTC TGTGGTGCTC 
TTTGGTCTGG TCTACAACCC ACACGAGGCC TGCTCCCTGA ACCTAGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTATGT GCTCTACGTG 
CGACTGGCCA TCTTGGTGAT CGAGTTCATC TATGCTATTG TGGGTATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCCAAGAACG TCACCCTTGG AATGGTTGTC 
TGCAACTGGG TGGTTATCCT CAGCGTCTGC ATTACTGTCC TCTGTGTCTT TGACCCCACG 
GGCCGCACGT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAACCT GCGGACTTAC 
AATCTGCGGC ACCGCTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACCCGGAC AAAGGACTCC CAGTCAGATG CCTACTCAGA AATTGCCTAC 
CTCTTCGCTG AATTTTTCCG AGACCTCGAC ATCGTGCCCT CTGACATCAT CGCCGGCCTA 
GTGCTGCTCA GGCAGCGGCA GCGAGCCAAG CGCAATGCGG TGCTGGATGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT GCGCTACAAA GAAGTCTGCT ATTATATGCT CTTCGCCCTG 
GCTGCCTATG GCTGGCCTAT GTATCTGATG CGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG CCTCTGCCCT GCACGGCCCC GATTCGCCCC TGGAGTCACC 
ATTGAGGAAG ACAACTGTTG TGGTTGCAAT GCCATCGCCA TCCGCCGCCA CTTCCTGGAT 
GAGAACATGA CTGCGGTGGA CATCGTTTAC ACCTCCTGTC ATGATGCGGT CTATGAAACT 
CCCTTCTATG TGGCAGTGGA CCATGACAAG AAGAAGGTGG TGATCAGTAT CCGTGGAACC 
CTGTCCCCTA AGGATGCCCT GACTGACCTG ACAGGTGATG CTGAGCGCCT CCCTGTGGAG 
GGACATCGAG GCACCTGGCT GGGCCATAAG GGCATGGTGC TGTCAGCAGA ATACATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTATCC CAGGCCTTCG GGAGAGACCT GGGCCGCGGA 
ACCAAACATT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCTGGCAC AGCCGCTATT 
CTCTCCTTCC TCCTGCGCCC TCAGTACCCG ACCCTCAAGT GCTTCGCCTA CTCACCCCCA 
GGGGGCCTGC TGAGTGAGGA TGCCATGGAA TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTGCC CAGGATTGGT CTTTCTCAGC TGGAGGGTTT CCGCCGGCAG 
CTCCTGGATG TCCTGCAGCG AAGTACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGCATCC CCAAGTCAGA GCTGCCGGAG GAGGTGGAGG TGACCACCCT GGCCAGCACA 
CGGCTCTGGA CCCACCCCAG TGATCTGACC ATTGCCCTTT CGGCCAGCAC CCCGCTCTAC 
CCACCGGGCC GCATCATCCA TGTGGTTCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAACAGGAGG AGCCCACCTA CTTTGCCATC TGGGGTGACA ACAAAGCCTT CAATGAGGTG 
ATCATTTCAC CGGCCATGCT CCATGAGCAC CTGCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACGGCACTGC TCTCTGCTGC GAAGGTCATG 
GTAAGCCCCA CCGAGGTGGA TCTGACTCCG GAGCTCATCT TCCAGCAGCA GCCTCTGCCC 
ACGGGGCCGC CCCTGCCCAC TGGCCTGGCC CTGGAGCTGC CTGCTGCAGA CCACCGCAAC 
AGCAGTGTCA GGAGCAAGTC TCAGTCTGAG ATGAGCCTGG AGGGCTTCTC CGAAGGGCGG 
CTGCTGTCCC CAGTGGCCGC AGCATCGGCA GCCCGCCAAG ACCCTGTAGA GCTGCTGCTG 
CTGTCCACCC AGGAGCGGCT GGCAGCAGAG CTGCAGGCCC GGCGGGCACC ATTGGCCACT 
ATGGAGAGCC TCTCAGACAC AGAGTCACTG TACAGCTTCG ACTCCCGCCG CTCCTCGGGC 
TTCCGCAGCA TCCGAGGCTC ACCCAGTCTC CACGCTGTAC TAGAGAGAGA TGAGGGTCAC 
CTCTTTTACA TTGACCCTGC CATCCCCGAG GAGAACCCAT CCCTCAGCTC CCGAACAGAG 
CTGCTGGCAG CAGACAGCCT GTCCAAACAC TCACAGGACA CGCAGCCCCT GGAGGCGGCT 
CTGGGCAGCG GGGGTGTCAC TCCTGAGCGG CCACCAAGTG CAGTCAATGA GGAGGAGGAG 
GCAGCAGCGG GTGGAGAAGG TGGTGGAGCG GCACCGCGAG GAGAGCTGGC GCTGCACAAC 
GGGCGTCTGG GGGACTCGCC CAGCCCTCAG GTACTGGAGT TCGCTGAGTT CATCGACAGC 
CTCTTCAACC TCGACAGCAA GAGCAGTTCC TTTCAGGACC TCTACTGCAT GGTGGTGCCC 
GAGAGCCCCA CTAGCGACTA TACTGAGGGC CCCAAGTCTC CCAGCCAGCA GGAGATTCTG 
CTTCGGGCCC AGTTTGAGCC CAATCTGGTG CCCAAGCCCC CTCGCCTCTT TGCAGGCTCT 
GCTGACCCCT CCTCTGGCAT CTCGCTCTCA CCCTCCTTCC CACTCAGCTC CTCCGGGGAG 
CTCATGGACC TGACGCCCAC TGGCCTCAGC AGCCAGGAGT GCTTGGCTGC AGATAAGATC 
CGGACTTCCA CCCCCACAGG CCATGGGGCC AGCCCTACTA AGCAGGATGA CCTGGTCATC 
TCAGCACGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008825328.1
CDS	106..3237
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPLPTGLALELPAADHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAVNEEEEAAAGGEGGGAAPRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_008827106.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili diacylglycerol lipase alpha (Dagla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008827106.1

ORF Insert Sequence:

ATGCCCGGGA TTGTGGTGTT CCGCCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCTTG 
CCAGCCATCT TTCTCTTCCT CCTGCACACC ACCTGGTTTG TGATCCTGTC TGTGGTGCTC 
TTTGGTCTGG TCTACAACCC ACACGAGGCC TGCTCCCTGA ACCTAGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTATGT GCTCTACGTG 
CGACTGGCCA TCTTGGTGAT CGAGTTCATC TATGCTATTG TGGGTATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCCAAGAACG TCACCCTTGG AATGGTTGTC 
TGCAACTGGG TGGTTATCCT CAGCGTCTGC ATTACTGTCC TCTGTGTCTT TGACCCCACG 
GGCCGCACGT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAACCT GCGGACTTAC 
AATCTGCGGC ACCGCTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACCCGGAC AAAGGACTCC CAGTCAGATG CCTACTCAGA AATTGCCTAC 
CTCTTCGCTG AATTTTTCCG AGACCTCGAC ATCGTGCCCT CTGACATCAT CGCCGGCCTA 
GTGCTGCTCA GGCAGCGGCA GCGAGCCAAG CGCAATGCGG TGCTGGATGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT GCGCTACAAA GAAGTCTGCT ATTATATGCT CTTCGCCCTG 
GCTGCCTATG GCTGGCCTAT GTATCTGATG CGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG CCTCTGCCCT GCACGGCCCC GATTCGCCCC TGGAGTCACC 
ATTGAGGAAG ACAACTGTTG TGGTTGCAAT GCCATCGCCA TCCGCCGCCA CTTCCTGGAT 
GAGAACATGA CTGCGGTGGA CATCGTTTAC ACCTCCTGTC ATGATGCGGT CTATGAAACT 
CCCTTCTATG TGGCAGTGGA CCATGACAAG AAGAAGGTGG TGATCAGTAT CCGTGGAACC 
CTGTCCCCTA AGGATGCCCT GACTGACCTG ACAGGTGATG CTGAGCGCCT CCCTGTGGAG 
GGACATCGAG GCACCTGGCT GGGCCATAAG GGCATGGTGC TGTCAGCAGA ATACATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTATCC CAGGCCTTCG GGAGAGACCT GGGCCGCGGA 
ACCAAACATT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCTGGCAC AGCCGCTATT 
CTCTCCTTCC TCCTGCGCCC TCAGTACCCG ACCCTCAAGT GCTTCGCCTA CTCACCCCCA 
GGGGGCCTGC TGAGTGAGGA TGCCATGGAA TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTGCC CAGGATTGGT CTTTCTCAGC TGGAGGGTTT CCGCCGGCAG 
CTCCTGGATG TCCTGCAGCG AAGTACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGCATCC CCAAGTCAGA GCTGCCGGAG GAGGTGGAGG TGACCACCCT GGCCAGCACA 
CGGCTCTGGA CCCACCCCAG TGATCTGACC ATTGCCCTTT CGGCCAGCAC CCCGCTCTAC 
CCACCGGGCC GCATCATCCA TGTGGTTCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAACAGGAGG AGCCCACCTA CTTTGCCATC TGGGGTGACA ACAAAGCCTT CAATGAGGTG 
ATCATTTCAC CGGCCATGCT CCATGAGCAC CTGCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACGGCACTGC TCTCTGCTGC GAAGGTCATG 
GTAAGCCCCA CCGAGGTGGA TCTGACTCCG GAGCTCATCT TCCAGCAGCA GCCTCTGCCC 
ACGGGGCCGC CCCTGCCCAC TGGCCTGGCC CTGGAGCTGC CTGCTGCAGA CCACCGCAAC 
AGCAGTGTCA GGAGCAAGTC TCAGTCTGAG ATGAGCCTGG AGGGCTTCTC CGAAGGGCGG 
CTGCTGTCCC CAGTGGCCGC AGCATCGGCA GCCCGCCAAG ACCCTGTAGA GCTGCTGCTG 
CTGTCCACCC AGGAGCGGCT GGCAGCAGAG CTGCAGGCCC GGCGGGCACC ATTGGCCACT 
ATGGAGAGCC TCTCAGACAC AGAGTCACTG TACAGCTTCG ACTCCCGCCG CTCCTCGGGC 
TTCCGCAGCA TCCGAGGCTC ACCCAGTCTC CACGCTGTAC TAGAGAGAGA TGAGGGTCAC 
CTCTTTTACA TTGACCCTGC CATCCCCGAG GAGAACCCAT CCCTCAGCTC CCGAACAGAG 
CTGCTGGCAG CAGACAGCCT GTCCAAACAC TCACAGGACA CGCAGCCCCT GGAGGCGGCT 
CTGGGCAGCG GGGGTGTCAC TCCTGAGCGG CCACCAAGTG CAGTCAATGA GGAGGAGGAG 
GCAGCAGCGG GTGGAGAAGG TGGTGGAGCG GCACCGCGAG GAGAGCTGGC GCTGCACAAC 
GGGCGTCTGG GGGACTCGCC CAGCCCTCAG GTACTGGAGT TCGCTGAGTT CATCGACAGC 
CTCTTCAACC TCGACAGCAA GAGCAGTTCC TTTCAGGACC TCTACTGCAT GGTGGTGCCC 
GAGAGCCCCA CTAGCGACTA TACTGAGGGC CCCAAGTCTC CCAGCCAGCA GGAGATTCTG 
CTTCGGGCCC AGTTTGAGCC CAATCTGGTG CCCAAGCCCC CTCGCCTCTT TGCAGGCTCT 
GCTGACCCCT CCTCTGGCAT CTCGCTCTCA CCCTCCTTCC CACTCAGCTC CTCCGGGGAG 
CTCATGGACC TGACGCCCAC TGGCCTCAGC AGCCAGGAGT GCTTGGCTGC AGATAAGATC 
CGGACTTCCA CCCCCACAGG CCATGGGGCC AGCCCTACTA AGCAGGATGA CCTGGTCATC 
TCAGCACGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dagla ( XM_008827106.2 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_008825328.1 Nannospalax galili diacylglycerol lipase alpha (Dagla), mRNA.

Price & Availability↑

CloneID	ONa44147
Clone ID Related Accession (Same CDS sequence)	XM_008827106.1 , XM_008827106.2
Accession Version	XM_008827106.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	sn1-specific diacylglycerol lipase alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008331995.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2019 this sequence version replaced XM_008827106.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGA TTGTGGTGTT CCGCCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCTTG 
CCAGCCATCT TTCTCTTCCT CCTGCACACC ACCTGGTTTG TGATCCTGTC TGTGGTGCTC 
TTTGGTCTGG TCTACAACCC ACACGAGGCC TGCTCCCTGA ACCTAGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTATGT GCTCTACGTG 
CGACTGGCCA TCTTGGTGAT CGAGTTCATC TATGCTATTG TGGGTATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCCAAGAACG TCACCCTTGG AATGGTTGTC 
TGCAACTGGG TGGTTATCCT CAGCGTCTGC ATTACTGTCC TCTGTGTCTT TGACCCCACG 
GGCCGCACGT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAACCT GCGGACTTAC 
AATCTGCGGC ACCGCTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACCCGGAC AAAGGACTCC CAGTCAGATG CCTACTCAGA AATTGCCTAC 
CTCTTCGCTG AATTTTTCCG AGACCTCGAC ATCGTGCCCT CTGACATCAT CGCCGGCCTA 
GTGCTGCTCA GGCAGCGGCA GCGAGCCAAG CGCAATGCGG TGCTGGATGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT GCGCTACAAA GAAGTCTGCT ATTATATGCT CTTCGCCCTG 
GCTGCCTATG GCTGGCCTAT GTATCTGATG CGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG CCTCTGCCCT GCACGGCCCC GATTCGCCCC TGGAGTCACC 
ATTGAGGAAG ACAACTGTTG TGGTTGCAAT GCCATCGCCA TCCGCCGCCA CTTCCTGGAT 
GAGAACATGA CTGCGGTGGA CATCGTTTAC ACCTCCTGTC ATGATGCGGT CTATGAAACT 
CCCTTCTATG TGGCAGTGGA CCATGACAAG AAGAAGGTGG TGATCAGTAT CCGTGGAACC 
CTGTCCCCTA AGGATGCCCT GACTGACCTG ACAGGTGATG CTGAGCGCCT CCCTGTGGAG 
GGACATCGAG GCACCTGGCT GGGCCATAAG GGCATGGTGC TGTCAGCAGA ATACATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTATCC CAGGCCTTCG GGAGAGACCT GGGCCGCGGA 
ACCAAACATT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCTGGCAC AGCCGCTATT 
CTCTCCTTCC TCCTGCGCCC TCAGTACCCG ACCCTCAAGT GCTTCGCCTA CTCACCCCCA 
GGGGGCCTGC TGAGTGAGGA TGCCATGGAA TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTGCC CAGGATTGGT CTTTCTCAGC TGGAGGGTTT CCGCCGGCAG 
CTCCTGGATG TCCTGCAGCG AAGTACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGCATCC CCAAGTCAGA GCTGCCGGAG GAGGTGGAGG TGACCACCCT GGCCAGCACA 
CGGCTCTGGA CCCACCCCAG TGATCTGACC ATTGCCCTTT CGGCCAGCAC CCCGCTCTAC 
CCACCGGGCC GCATCATCCA TGTGGTTCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAACAGGAGG AGCCCACCTA CTTTGCCATC TGGGGTGACA ACAAAGCCTT CAATGAGGTG 
ATCATTTCAC CGGCCATGCT CCATGAGCAC CTGCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACGGCACTGC TCTCTGCTGC GAAGGTCATG 
GTAAGCCCCA CCGAGGTGGA TCTGACTCCG GAGCTCATCT TCCAGCAGCA GCCTCTGCCC 
ACGGGGCCGC CCCTGCCCAC TGGCCTGGCC CTGGAGCTGC CTGCTGCAGA CCACCGCAAC 
AGCAGTGTCA GGAGCAAGTC TCAGTCTGAG ATGAGCCTGG AGGGCTTCTC CGAAGGGCGG 
CTGCTGTCCC CAGTGGCCGC AGCATCGGCA GCCCGCCAAG ACCCTGTAGA GCTGCTGCTG 
CTGTCCACCC AGGAGCGGCT GGCAGCAGAG CTGCAGGCCC GGCGGGCACC ATTGGCCACT 
ATGGAGAGCC TCTCAGACAC AGAGTCACTG TACAGCTTCG ACTCCCGCCG CTCCTCGGGC 
TTCCGCAGCA TCCGAGGCTC ACCCAGTCTC CACGCTGTAC TAGAGAGAGA TGAGGGTCAC 
CTCTTTTACA TTGACCCTGC CATCCCCGAG GAGAACCCAT CCCTCAGCTC CCGAACAGAG 
CTGCTGGCAG CAGACAGCCT GTCCAAACAC TCACAGGACA CGCAGCCCCT GGAGGCGGCT 
CTGGGCAGCG GGGGTGTCAC TCCTGAGCGG CCACCAAGTG CAGTCAATGA GGAGGAGGAG 
GCAGCAGCGG GTGGAGAAGG TGGTGGAGCG GCACCGCGAG GAGAGCTGGC GCTGCACAAC 
GGGCGTCTGG GGGACTCGCC CAGCCCTCAG GTACTGGAGT TCGCTGAGTT CATCGACAGC 
CTCTTCAACC TCGACAGCAA GAGCAGTTCC TTTCAGGACC TCTACTGCAT GGTGGTGCCC 
GAGAGCCCCA CTAGCGACTA TACTGAGGGC CCCAAGTCTC CCAGCCAGCA GGAGATTCTG 
CTTCGGGCCC AGTTTGAGCC CAATCTGGTG CCCAAGCCCC CTCGCCTCTT TGCAGGCTCT 
GCTGACCCCT CCTCTGGCAT CTCGCTCTCA CCCTCCTTCC CACTCAGCTC CTCCGGGGAG 
CTCATGGACC TGACGCCCAC TGGCCTCAGC AGCCAGGAGT GCTTGGCTGC AGATAAGATC 
CGGACTTCCA CCCCCACAGG CCATGGGGCC AGCCCTACTA AGCAGGATGA CCTGGTCATC 
TCAGCACGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008825328.1
CDS	176..3307
Translation	MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCMIAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVVCNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDSQSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDLKNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCNAIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVEGHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYPTLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGATKCIPKSELPEEVEVTTLASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFAIWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPLPTGPPLPTGLALELPAADHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAAELQARRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRTELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAVNEEEEAAAGGEGGGAAPRGELALHNGRLGDSPSPQVLEFAEFIDSLFNLDSKSSSFQDLYCMVVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAGSADPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLAADKIRTSTPTGHGASPTKQDDLVISAR

Target ORF information:

RefSeq Version	XM_008827106.2
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili diacylglycerol lipase alpha (Dagla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008827106.2

ORF Insert Sequence:

ATGCCCGGGA TTGTGGTGTT CCGCCGGCGC TGGTCTGTGG GCAGCGATGA CCTCGTCTTG 
CCAGCCATCT TTCTCTTCCT CCTGCACACC ACCTGGTTTG TGATCCTGTC TGTGGTGCTC 
TTTGGTCTGG TCTACAACCC ACACGAGGCC TGCTCCCTGA ACCTAGTGGA CCACGGCCGC 
GGCTACCTGG GCATCCTGCT GAGCTGCATG ATCGCCGAGA TGGCCATCAT CTGGCTGAGC 
ATGCGTGGGG GCATCCTCTA CACGGAGCCC CGTGACTCCA TGCAGTATGT GCTCTACGTG 
CGACTGGCCA TCTTGGTGAT CGAGTTCATC TATGCTATTG TGGGTATCGT CTGGCTCACA 
CAGTACTACA CCTCCTGCAA TGACCTCACT GCCAAGAACG TCACCCTTGG AATGGTTGTC 
TGCAACTGGG TGGTTATCCT CAGCGTCTGC ATTACTGTCC TCTGTGTCTT TGACCCCACG 
GGCCGCACGT TCGTCAAGCT GAGAGCCACC AAGAGGAGGC AGCGCAACCT GCGGACTTAC 
AATCTGCGGC ACCGCTTAGA GGAGGGTCAG GCCACCAGCT GGTCCCGCCG GCTCAAAGTG 
TTCCTCTGCT GCACCCGGAC AAAGGACTCC CAGTCAGATG CCTACTCAGA AATTGCCTAC 
CTCTTCGCTG AATTTTTCCG AGACCTCGAC ATCGTGCCCT CTGACATCAT CGCCGGCCTA 
GTGCTGCTCA GGCAGCGGCA GCGAGCCAAG CGCAATGCGG TGCTGGATGA GGCAAACAAT 
GACATCTTGG CTTTCCTGTC TGGGATGCCA GTGACCAGAA ACACCAAGTA CCTCGACCTC 
AAGAACTCGC ACGAGATGCT GCGCTACAAA GAAGTCTGCT ATTATATGCT CTTCGCCCTG 
GCTGCCTATG GCTGGCCTAT GTATCTGATG CGGAAGCCCA CCTGTGGTCT CTGCCAGCTG 
GCTCGGTCCT GCTCGTGCTG CCTCTGCCCT GCACGGCCCC GATTCGCCCC TGGAGTCACC 
ATTGAGGAAG ACAACTGTTG TGGTTGCAAT GCCATCGCCA TCCGCCGCCA CTTCCTGGAT 
GAGAACATGA CTGCGGTGGA CATCGTTTAC ACCTCCTGTC ATGATGCGGT CTATGAAACT 
CCCTTCTATG TGGCAGTGGA CCATGACAAG AAGAAGGTGG TGATCAGTAT CCGTGGAACC 
CTGTCCCCTA AGGATGCCCT GACTGACCTG ACAGGTGATG CTGAGCGCCT CCCTGTGGAG 
GGACATCGAG GCACCTGGCT GGGCCATAAG GGCATGGTGC TGTCAGCAGA ATACATCAAG 
AAGAAGCTGG AGCAGGAGAT GGTCCTATCC CAGGCCTTCG GGAGAGACCT GGGCCGCGGA 
ACCAAACATT ACGGCCTGAT TGTGGTGGGC CACTCCCTGG GCGCTGGCAC AGCCGCTATT 
CTCTCCTTCC TCCTGCGCCC TCAGTACCCG ACCCTCAAGT GCTTCGCCTA CTCACCCCCA 
GGGGGCCTGC TGAGTGAGGA TGCCATGGAA TACTCCAAGG AGTTTGTGAC TGCTGTGGTT 
CTGGGCAAAG ACCTCGTGCC CAGGATTGGT CTTTCTCAGC TGGAGGGTTT CCGCCGGCAG 
CTCCTGGATG TCCTGCAGCG AAGTACCAAG CCCAAATGGC GGATCATCGT GGGGGCCACC 
AAGTGCATCC CCAAGTCAGA GCTGCCGGAG GAGGTGGAGG TGACCACCCT GGCCAGCACA 
CGGCTCTGGA CCCACCCCAG TGATCTGACC ATTGCCCTTT CGGCCAGCAC CCCGCTCTAC 
CCACCGGGCC GCATCATCCA TGTGGTTCAC AACCACCCTG CAGAGCAGTG CTGCTGCTGT 
GAACAGGAGG AGCCCACCTA CTTTGCCATC TGGGGTGACA ACAAAGCCTT CAATGAGGTG 
ATCATTTCAC CGGCCATGCT CCATGAGCAC CTGCCCTACG TGGTCATGGA GGGGCTCAAC 
AAGGTGCTGG AGAACTACAA CAAGGGGAAG ACGGCACTGC TCTCTGCTGC GAAGGTCATG 
GTAAGCCCCA CCGAGGTGGA TCTGACTCCG GAGCTCATCT TCCAGCAGCA GCCTCTGCCC 
ACGGGGCCGC CCCTGCCCAC TGGCCTGGCC CTGGAGCTGC CTGCTGCAGA CCACCGCAAC 
AGCAGTGTCA GGAGCAAGTC TCAGTCTGAG ATGAGCCTGG AGGGCTTCTC CGAAGGGCGG 
CTGCTGTCCC CAGTGGCCGC AGCATCGGCA GCCCGCCAAG ACCCTGTAGA GCTGCTGCTG 
CTGTCCACCC AGGAGCGGCT GGCAGCAGAG CTGCAGGCCC GGCGGGCACC ATTGGCCACT 
ATGGAGAGCC TCTCAGACAC AGAGTCACTG TACAGCTTCG ACTCCCGCCG CTCCTCGGGC 
TTCCGCAGCA TCCGAGGCTC ACCCAGTCTC CACGCTGTAC TAGAGAGAGA TGAGGGTCAC 
CTCTTTTACA TTGACCCTGC CATCCCCGAG GAGAACCCAT CCCTCAGCTC CCGAACAGAG 
CTGCTGGCAG CAGACAGCCT GTCCAAACAC TCACAGGACA CGCAGCCCCT GGAGGCGGCT 
CTGGGCAGCG GGGGTGTCAC TCCTGAGCGG CCACCAAGTG CAGTCAATGA GGAGGAGGAG 
GCAGCAGCGG GTGGAGAAGG TGGTGGAGCG GCACCGCGAG GAGAGCTGGC GCTGCACAAC 
GGGCGTCTGG GGGACTCGCC CAGCCCTCAG GTACTGGAGT TCGCTGAGTT CATCGACAGC 
CTCTTCAACC TCGACAGCAA GAGCAGTTCC TTTCAGGACC TCTACTGCAT GGTGGTGCCC 
GAGAGCCCCA CTAGCGACTA TACTGAGGGC CCCAAGTCTC CCAGCCAGCA GGAGATTCTG 
CTTCGGGCCC AGTTTGAGCC CAATCTGGTG CCCAAGCCCC CTCGCCTCTT TGCAGGCTCT 
GCTGACCCCT CCTCTGGCAT CTCGCTCTCA CCCTCCTTCC CACTCAGCTC CTCCGGGGAG 
CTCATGGACC TGACGCCCAC TGGCCTCAGC AGCCAGGAGT GCTTGGCTGC AGATAAGATC 
CGGACTTCCA CCCCCACAGG CCATGGGGCC AGCCCTACTA AGCAGGATGA CCTGGTCATC 
TCAGCACGCT AG