Inf2 cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Inf2
Entrez Gene ID	103724396
Full Name	inverted formin, FH2 and WH2 domain containing
Gene Type	protein-coding
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017795172.1	XP_017650661.1	inverted formin-2
XM_029557826.1	XP_029413686.1	inverted formin-2

Pathways from BioSystems
Gene Ontology

The following Inf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Inf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONa138131	XM_029557826.1 Latest version!	Nannospalax galili inverted formin, FH2 and WH2 domain containing (Inf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONa38711	XM_017795172.1 Latest version!	Nannospalax galili inverted formin, FH2 and WH2 domain containing (Inf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Inf2 ( XM_029557826.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_029413686.1 Nannospalax galili inverted formin, FH2 and WH2 domain containing (Inf2), mRNA.

Price & Availability↑

CloneID	ONa138131
Clone ID Related Accession (Same CDS sequence)	XM_029557826.1
Accession Version	XM_029557826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	inverted formin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358189.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nannospalax galili Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGTGA AGGAGGGTGC ACAGCGCAAG TGGGCGGCGC TGAAGGAGAA GCTGGGGCCT 
CAGGACTCTG ACCCCACTGA AGCCAACCTG GAGAGCGCAG AGCCAGAGCT GTGCATCCGG 
CTGCTGCAGA TGCCCTCTGT GGTCAACTAC TCTGGCCTGC GAAAGCGCCT GGAGAGCAGT 
GATGGTGGCT GGATGGTGCA GTTCCTGGAG CAGAGTGGCC TGGACCTGCT GCTAGAGGCA 
CTGGCGCGGC TGTCCGGGCG TGGTGTGGCC CGCATCTCGG ATGCCCTGCT ACAGCTTACC 
TGTATCAGCT GCGTGCGTGC CGTCATGAAC TCACAGCAGG GCATAGAGTA CATTCTCAGC 
AACCAGGGCT ATGTGCGCCA GCTCTCCCAG GCTCTGGACA CGTCCAATGT GATGGTCAAG 
AAGCAGGTGT TTGAGCTGCT GGCTGCCCTG TGCATCTATT CACCTGAGGG CCACGCACTG 
ACACTAGATG CCCTGGACCA TTACAAGACG GTGTGCAGCC AGCAGTACCG CTTCAGCGTC 
ATCATGAATG AGCTCTCCGA CAGCGACAAC GTGCCCTATG TGGTCACCCT GCTCAGCGTG 
ATCAATGCCA TCATCCTGGG CCCCGAGGAT CTCCGCACTC GTGCCCAGCT TCGGAGTGAA 
TTCATTGGGC TCCAGCTGCT GGACATTCTG ACCCGGCTGC GGGACCTAGA GGATGCCGAC 
CTGCTGATCC AGCTAGAGGC CTTTGAGGAG GCCAAAGCTG AAGATGAGGA GGAGCTGCAA 
CGTGTCTGTG ATGGTGTCAA CATGAACAGC CACCAGGAAG TCTTCGCCTC TCTGTTCCAC 
AAGGTGAGCT GTTCCCCAGC ATCAGCCCAG CTGCTGTCCG TGTTACAAGG CCTCTTACAC 
CTGGAGCCTG CTGGCCGCTC GGGCCAGCTG CTCTGGGAGG CCCTAGAGAA CCTGGTGAAC 
CGGGCCGTGC TCCTGGCCAG TGATGCACAG GCTTGTACCC TGGAGGAAGT GGTGGAGCGA 
CTGCTGTCCA TCAAAGGGCG GCCCCGACCA AGCCCCCTGG ACAAGGCCCA CAAGGGCGTT 
CAGGCCAACT TGGGCCAAAG TCGGAGGGAC AAGTCCCCCC AAAACACTAC TACCCCCATG 
GCCAAAATGG AGGGCCAACA ACCAATTGTG GCTTCTGCCT GCGAGTGTGT GGTCAGCGTC 
CAGAGCTCCA GTGGTGACAC AGCCCCACAG CCATTAGTCC TTGAGCAGAG GGTGCCTACC 
CCACCTCTGC CCAGCCCCCC ACTCCCAGGC ATGGGGTGCC CACCTCCTCC CCCTCCCCCA 
CTACCTGGAA TAGGCTTCGG CCCCCCTCCA CCTCCTCCCC TGCCTGGTAT GTCCATCCCC 
TCCATGGTGG GTGGCGTGGA GGAGATCATT GTGGCCCAGG TGGACCACAG CTTGGGCTCA 
GCCTGGGTCC CCAGTCACCG CCGGGTGAAT CCACCCACAC TGCGCATGAA GAAGTTGAAC 
TGGCAAAAGC TGCCATCCAA CGTGGCACAA GAGCGCAGCT CTATGTGGGC AGCTCTGAGC 
AGCCCCTGCA CGGAGGGGGT GGAACCTGAC TTCTCCAGCA TCGAACAGCT GTTCTCCTTC 
CCCACCGCCA AGCCCAAGGA GCCCTCTGCT GCCCCAGCGA GGAAGGAGCC GAAGGAGGTC 
ACGTTTTTGG ACTCCAAGAA GAGCCTGAAC CTCAACATCT TCCTGAAGCA ATTTAAATGC 
TCCAATGAGG AGGTCACCAC CATGATCCAG GCTGGGGATA CAACCAAGTT TGATGTGGAG 
GTTCTCAAAC AGCTCCTGAA GCTCCTTCCA GAGAAACATG AAATTGAGAA CCTTCGGGCA 
TTCAAGGAGG AGCGAACCAA GCTGGCCAAT GCTGACCAGT TCTATGTCCT CCTGCTGGAC 
ATTCCTTGCT ACCAGCTGCG GGTGGAGTGC ATGCTGCTGT GCGAGGGCAC TGCCGTTGTG 
CTGGACATGG TGCGGCCCAA GGCCCAGCTG GTGCTCACCG CCTGTGAGAG CCTGCTCACC 
AGTCATCGGC TACCCATCTT CTGCCAGCTG ATCCTGAAAA TTGGGAACTT TCTCAACTAC 
GGCAGCCACA CGGGAGACGC AGATGGCTTC AAGATCAGCA CGTTGTTGAA ACTCACGGAG 
ACCAAGTCCC AGCAGAGCCG TGTGACACTG CTGCACCACG TGCTGGAGGA AGTGGAGAAG 
AGCCACCCTG ACCTCCTACA GCTGCCCCAG GACCTGGAGC CACCCTCCCA AGCTGCTGGG 
ATCAACTTGG AGATCATCCG CTCAGAGGCC AGTGCCAACC TGAAGAAGCT TCTAGAAACA 
GAGCAGAAGG TGTCCACCTC GGTCCCTGAG GTGCAAGAGC AGTATTCCAA GCGCCTGCAG 
GCCAGCATTG AGGCCTCTCA GGCACTGGAC AAGGTGTTTG AGGCCATTGA GCGGAAGAAG 
CTGCAGTTGG CTGATTACCT GTGTGAAGAC CCCCAGCAGC TGTCCCTGGA GGACACGTTC 
AGTACCATGA AGACCTTCCG TGACCTCTTT ACCCGTGCAC TGAAGGAGAA TAAGGACCGG 
AAGGAGCAGA TGGTAAAGGC AGAGAGGAGA AAACAGCAGT TGGCAGAGGA GGAGGCACGG 
AGGCCAAGGG GCGAGGACGG GAAGCCTGTC AGGAAGGGGC CGGGAAGGCA GGAAGAGGTG 
TGTGTCATCG ATGCCCTGCT GGCCGACATC AGGAAGGGCT TTCAGCTGCG GAAGACAGCC 
CGTGGTCGAG GCGATACTGA GGCGGGTAGC AAAGTGACCC CGGCAGACCC CCTGAAGCCC 
ACAGAGCCTG CAGCTGACAA CGATCCTAGA CAAGGCCCAG CGTGTGGCAC AAGTCACCCT 
GCCACAGAGC CAGGCCTGGA TGCCACAGCA GCTAGTGAGC CACAGGGCTG GGACCTTGTG 
GATGCCATGA CCCCCAGTCC TCTGCCCTCG GAGGAGGGTG GTCCCCCAGC CCTGGAGAGG 
CGTTCCTCCT GGTATGTTGA TGCCAGCGAC TTCCTGGTCC CCGAGGACAC CCTGAATGCC 
CAACCCTCCA CGGGGACCTG GCCAGTGACT CTGGGAGATG ATCAGTCCCT GAAGCCTCTT 
GAGTTCTCCA GCAATAAGCC CCCTGGGGCC GAGAACTCAC ACCAGGATGC TGCGGACCCT 
GAAGTCTTGT GGGGCATCCA TCAGGCCAAG GCAGACAGTA CAAGTGAGGG ACTAGAGGGC 
ACACCTGAGT ACAGCCGCGG CGCCCACCTG CCCGACACAG GCCCTGTCAG GGATGAGGAT 
GAGGACGACA CAGCCCCAGA ATCTGCGCTG GACACGTCTC TGGACAAGTC CCTCTCTGAG 
GATGCAGTGA CAGATTCCTC AGGGTCTGGC ACCCTCCCCA GGGACCGAGG GCGAGCCTCA 
AAAGGGGCAA GCAGGGGAAG GAAGAAGCGT CCCACAAGGA ACCAGGAAGA AGTTGCCCCT 
GATTCTGATG CTAATAAAAC AAAAAGGTTG TGTGTGATCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029413686.1
CDS	168..3692
Translation	MSVKEGAQRKWAALKEKLGPQDSDPTEANLESAEPELCIRLLQMPSVVNYSGLRKRLESSDGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCISCVRAVMNSQQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCIYSPEGHALTLDALDHYKTVCSQQYRFSVIMNELSDSDNVPYVVTLLSVINAIILGPEDLRTRAQLRSEFIGLQLLDILTRLRDLEDADLLIQLEAFEEAKAEDEEELQRVCDGVNMNSHQEVFASLFHKVSCSPASAQLLSVLQGLLHLEPAGRSGQLLWEALENLVNRAVLLASDAQACTLEEVVERLLSIKGRPRPSPLDKAHKGVQANLGQSRRDKSPQNTTTPMAKMEGQQPIVASACECVVSVQSSSGDTAPQPLVLEQRVPTPPLPSPPLPGMGCPPPPPPPLPGIGFGPPPPPPLPGMSIPSMVGGVEEIIVAQVDHSLGSAWVPSHRRVNPPTLRMKKLNWQKLPSNVAQERSSMWAALSSPCTEGVEPDFSSIEQLFSFPTAKPKEPSAAPARKEPKEVTFLDSKKSLNLNIFLKQFKCSNEEVTTMIQAGDTTKFDVEVLKQLLKLLPEKHEIENLRAFKEERTKLANADQFYVLLLDIPCYQLRVECMLLCEGTAVVLDMVRPKAQLVLTACESLLTSHRLPIFCQLILKIGNFLNYGSHTGDADGFKISTLLKLTETKSQQSRVTLLHHVLEEVEKSHPDLLQLPQDLEPPSQAAGINLEIIRSEASANLKKLLETEQKVSTSVPEVQEQYSKRLQASIEASQALDKVFEAIERKKLQLADYLCEDPQQLSLEDTFSTMKTFRDLFTRALKENKDRKEQMVKAERRKQQLAEEEARRPRGEDGKPVRKGPGRQEEVCVIDALLADIRKGFQLRKTARGRGDTEAGSKVTPADPLKPTEPAADNDPRQGPACGTSHPATEPGLDATAASEPQGWDLVDAMTPSPLPSEEGGPPALERRSSWYVDASDFLVPEDTLNAQPSTGTWPVTLGDDQSLKPLEFSSNKPPGAENSHQDAADPEVLWGIHQAKADSTSEGLEGTPEYSRGAHLPDTGPVRDEDEDDTAPESALDTSLDKSLSEDAVTDSSGSGTLPRDRGRASKGASRGRKKRPTRNQEEVAPDSDANKTKRLCVIQ

Target ORF information:

RefSeq Version	XM_029557826.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili inverted formin, FH2 and WH2 domain containing (Inf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029557826.1

ORF Insert Sequence:

ATGTCGGTGA AGGAGGGTGC ACAGCGCAAG TGGGCGGCGC TGAAGGAGAA GCTGGGGCCT 
CAGGACTCTG ACCCCACTGA AGCCAACCTG GAGAGCGCAG AGCCAGAGCT GTGCATCCGG 
CTGCTGCAGA TGCCCTCTGT GGTCAACTAC TCTGGCCTGC GAAAGCGCCT GGAGAGCAGT 
GATGGTGGCT GGATGGTGCA GTTCCTGGAG CAGAGTGGCC TGGACCTGCT GCTAGAGGCA 
CTGGCGCGGC TGTCCGGGCG TGGTGTGGCC CGCATCTCGG ATGCCCTGCT ACAGCTTACC 
TGTATCAGCT GCGTGCGTGC CGTCATGAAC TCACAGCAGG GCATAGAGTA CATTCTCAGC 
AACCAGGGCT ATGTGCGCCA GCTCTCCCAG GCTCTGGACA CGTCCAATGT GATGGTCAAG 
AAGCAGGTGT TTGAGCTGCT GGCTGCCCTG TGCATCTATT CACCTGAGGG CCACGCACTG 
ACACTAGATG CCCTGGACCA TTACAAGACG GTGTGCAGCC AGCAGTACCG CTTCAGCGTC 
ATCATGAATG AGCTCTCCGA CAGCGACAAC GTGCCCTATG TGGTCACCCT GCTCAGCGTG 
ATCAATGCCA TCATCCTGGG CCCCGAGGAT CTCCGCACTC GTGCCCAGCT TCGGAGTGAA 
TTCATTGGGC TCCAGCTGCT GGACATTCTG ACCCGGCTGC GGGACCTAGA GGATGCCGAC 
CTGCTGATCC AGCTAGAGGC CTTTGAGGAG GCCAAAGCTG AAGATGAGGA GGAGCTGCAA 
CGTGTCTGTG ATGGTGTCAA CATGAACAGC CACCAGGAAG TCTTCGCCTC TCTGTTCCAC 
AAGGTGAGCT GTTCCCCAGC ATCAGCCCAG CTGCTGTCCG TGTTACAAGG CCTCTTACAC 
CTGGAGCCTG CTGGCCGCTC GGGCCAGCTG CTCTGGGAGG CCCTAGAGAA CCTGGTGAAC 
CGGGCCGTGC TCCTGGCCAG TGATGCACAG GCTTGTACCC TGGAGGAAGT GGTGGAGCGA 
CTGCTGTCCA TCAAAGGGCG GCCCCGACCA AGCCCCCTGG ACAAGGCCCA CAAGGGCGTT 
CAGGCCAACT TGGGCCAAAG TCGGAGGGAC AAGTCCCCCC AAAACACTAC TACCCCCATG 
GCCAAAATGG AGGGCCAACA ACCAATTGTG GCTTCTGCCT GCGAGTGTGT GGTCAGCGTC 
CAGAGCTCCA GTGGTGACAC AGCCCCACAG CCATTAGTCC TTGAGCAGAG GGTGCCTACC 
CCACCTCTGC CCAGCCCCCC ACTCCCAGGC ATGGGGTGCC CACCTCCTCC CCCTCCCCCA 
CTACCTGGAA TAGGCTTCGG CCCCCCTCCA CCTCCTCCCC TGCCTGGTAT GTCCATCCCC 
TCCATGGTGG GTGGCGTGGA GGAGATCATT GTGGCCCAGG TGGACCACAG CTTGGGCTCA 
GCCTGGGTCC CCAGTCACCG CCGGGTGAAT CCACCCACAC TGCGCATGAA GAAGTTGAAC 
TGGCAAAAGC TGCCATCCAA CGTGGCACAA GAGCGCAGCT CTATGTGGGC AGCTCTGAGC 
AGCCCCTGCA CGGAGGGGGT GGAACCTGAC TTCTCCAGCA TCGAACAGCT GTTCTCCTTC 
CCCACCGCCA AGCCCAAGGA GCCCTCTGCT GCCCCAGCGA GGAAGGAGCC GAAGGAGGTC 
ACGTTTTTGG ACTCCAAGAA GAGCCTGAAC CTCAACATCT TCCTGAAGCA ATTTAAATGC 
TCCAATGAGG AGGTCACCAC CATGATCCAG GCTGGGGATA CAACCAAGTT TGATGTGGAG 
GTTCTCAAAC AGCTCCTGAA GCTCCTTCCA GAGAAACATG AAATTGAGAA CCTTCGGGCA 
TTCAAGGAGG AGCGAACCAA GCTGGCCAAT GCTGACCAGT TCTATGTCCT CCTGCTGGAC 
ATTCCTTGCT ACCAGCTGCG GGTGGAGTGC ATGCTGCTGT GCGAGGGCAC TGCCGTTGTG 
CTGGACATGG TGCGGCCCAA GGCCCAGCTG GTGCTCACCG CCTGTGAGAG CCTGCTCACC 
AGTCATCGGC TACCCATCTT CTGCCAGCTG ATCCTGAAAA TTGGGAACTT TCTCAACTAC 
GGCAGCCACA CGGGAGACGC AGATGGCTTC AAGATCAGCA CGTTGTTGAA ACTCACGGAG 
ACCAAGTCCC AGCAGAGCCG TGTGACACTG CTGCACCACG TGCTGGAGGA AGTGGAGAAG 
AGCCACCCTG ACCTCCTACA GCTGCCCCAG GACCTGGAGC CACCCTCCCA AGCTGCTGGG 
ATCAACTTGG AGATCATCCG CTCAGAGGCC AGTGCCAACC TGAAGAAGCT TCTAGAAACA 
GAGCAGAAGG TGTCCACCTC GGTCCCTGAG GTGCAAGAGC AGTATTCCAA GCGCCTGCAG 
GCCAGCATTG AGGCCTCTCA GGCACTGGAC AAGGTGTTTG AGGCCATTGA GCGGAAGAAG 
CTGCAGTTGG CTGATTACCT GTGTGAAGAC CCCCAGCAGC TGTCCCTGGA GGACACGTTC 
AGTACCATGA AGACCTTCCG TGACCTCTTT ACCCGTGCAC TGAAGGAGAA TAAGGACCGG 
AAGGAGCAGA TGGTAAAGGC AGAGAGGAGA AAACAGCAGT TGGCAGAGGA GGAGGCACGG 
AGGCCAAGGG GCGAGGACGG GAAGCCTGTC AGGAAGGGGC CGGGAAGGCA GGAAGAGGTG 
TGTGTCATCG ATGCCCTGCT GGCCGACATC AGGAAGGGCT TTCAGCTGCG GAAGACAGCC 
CGTGGTCGAG GCGATACTGA GGCGGGTAGC AAAGTGACCC CGGCAGACCC CCTGAAGCCC 
ACAGAGCCTG CAGCTGACAA CGATCCTAGA CAAGGCCCAG CGTGTGGCAC AAGTCACCCT 
GCCACAGAGC CAGGCCTGGA TGCCACAGCA GCTAGTGAGC CACAGGGCTG GGACCTTGTG 
GATGCCATGA CCCCCAGTCC TCTGCCCTCG GAGGAGGGTG GTCCCCCAGC CCTGGAGAGG 
CGTTCCTCCT GGTATGTTGA TGCCAGCGAC TTCCTGGTCC CCGAGGACAC CCTGAATGCC 
CAACCCTCCA CGGGGACCTG GCCAGTGACT CTGGGAGATG ATCAGTCCCT GAAGCCTCTT 
GAGTTCTCCA GCAATAAGCC CCCTGGGGCC GAGAACTCAC ACCAGGATGC TGCGGACCCT 
GAAGTCTTGT GGGGCATCCA TCAGGCCAAG GCAGACAGTA CAAGTGAGGG ACTAGAGGGC 
ACACCTGAGT ACAGCCGCGG CGCCCACCTG CCCGACACAG GCCCTGTCAG GGATGAGGAT 
GAGGACGACA CAGCCCCAGA ATCTGCGCTG GACACGTCTC TGGACAAGTC CCTCTCTGAG 
GATGCAGTGA CAGATTCCTC AGGGTCTGGC ACCCTCCCCA GGGACCGAGG GCGAGCCTCA 
AAAGGGGCAA GCAGGGGAAG GAAGAAGCGT CCCACAAGGA ACCAGGAAGA AGTTGCCCCT 
GATTCTGATG CTAATAAAAC AAAAAGGTTG TGTGTGATCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Inf2 ( XM_017795172.1 ) cDNA ORF clone, Nannospalax galili(Upper Galilee mountains blind mole rat) -> XP_017650661.1 Nannospalax galili inverted formin, FH2 and WH2 domain containing (Inf2), mRNA.

Price & Availability↑

CloneID	ONa38711
Clone ID Related Accession (Same CDS sequence)	XM_017795172.1
Accession Version	XM_017795172.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Product	inverted formin-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008358189.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nannospalax galili Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCGGTGA AGGAGGGTGC ACAGCGCAAG TGGGCGGCGC TGAAGGAGAA GCTGGGGCCT 
CAGGACTCTG ACCCCACTGA AGCCAACCTG GAGAGCGCAG AGCCAGAGCT GTGCATCCGG 
CTGCTGCAGA TGCCCTCTGT GGTCAACTAC TCTGGCCTGC GAAAGCGCCT GGAGAGCAGT 
GATGGTGGCT GGATGGTGCA GTTCCTGGAG CAGAGTGGCC TGGACCTGCT GCTAGAGGCA 
CTGGCGCGGC TGTCCGGGCG TGGTGTGGCC CGCATCTCGG ATGCCCTGCT ACAGCTTACC 
TGTATCAGCT GCGTGCGTGC CGTCATGAAC TCACAGCAGG GCATAGAGTA CATTCTCAGC 
AACCAGGGCT ATGTGCGCCA GCTCTCCCAG GCTCTGGACA CGTCCAATGT GATGGTCAAG 
AAGCAGGTGT TTGAGCTGCT GGCTGCCCTG TGCATCTATT CACCTGAGGG CCACGCACTG 
ACACTAGATG CCCTGGACCA TTACAAGACG GTGTGCAGCC AGCAGTACCG CTTCAGCGTC 
ATCATGAATG AGCTCTCCGA CAGCGACAAC GTGCCCTATG TGGTCACCCT GCTCAGCGTG 
ATCAATGCCA TCATCCTGGG CCCCGAGGAT CTCCGCACTC GTGCCCAGCT TCGGAGTGAA 
TTCATTGGGC TCCAGCTGCT GGACATTCTG ACCCGGCTGC GGGACCTAGA GGATGCCGAC 
CTGCTGATCC AGCTAGAGGC CTTTGAGGAG GCCAAAGCTG AAGATGAGGA GGAGCTGCAA 
CGTGTCTGTG ATGGTGTCAA CATGAACAGC CACCAGGAAG TCTTCGCCTC TCTGTTCCAC 
AAGGTGAGCT GTTCCCCAGC ATCAGCCCAG CTGCTGTCCG TGTTACAAGG CCTCTTACAC 
CTGGAGCCTG CTGGCCGCTC GGGCCAGCTG CTCTGGGAGG CCCTAGAGAA CCTGGTGAAC 
CGGGCCGTGC TCCTGGCCAG TGATGCACAG GCTTGTACCC TGGAGGAAGT GGTGGAGCGA 
CTGCTGTCCA TCAAAGGGCG GCCCCGACCA AGCCCCCTGG ACAAGGCCCA CAAGGGCGTT 
CAGGCCAACT TGGGCCAAAG TCGGAGGGAC AAGTCCCCCC AAAACACTAC TACCCCCATG 
GCCAAAATGG AGGGCCAACA ACCAATTGTG GCTTCTGCCT GCGAGTGTGT GGTCAGCGTC 
CAGAGCTCCA GTGGTGACAC AGCCCCACAG CCATTAGTCC TTGAGCAGAG GGTGCCTACC 
CCACCTCTGC CCAGCCCCCC ACTCCCAGGC ATGGGGTGCC CACCTCCTCC CCCTCCCCCA 
CTACCTGGAA TAGGCTTCGG CCCCCCTCCA CCTCCTCCCC TGCCTGGTAT GTCCATCCCC 
TCCATGGTGG GTGGCGTGGA GGAGATCATT GTGGCCCAGG TGGACCACAG CTTGGGCTCA 
GCCTGGGTCC CCAGTCACCG CCGGGTGAAT CCACCCACAC TGCGCATGAA GAAGTTGAAC 
TGGCAAAAGC TGCCATCCAA CGTGGCACAA GAGCGCAGCT CTATGTGGGC AGCTCTGAGC 
AGCCCCTGCA CGGAGGGGGT GGAACCTGAC TTCTCCAGCA TCGAACAGCT GTTCTCCTTC 
CCCACCGCCA AGCCCAAGGA GCCCTCTGCT GCCCCAGCGA GGAAGGAGCC GAAGGAGGTC 
ACGTTTTTGG ACTCCAAGAA GAGCCTGAAC CTCAACATCT TCCTGAAGCA ATTTAAATGC 
TCCAATGAGG AGGTCACCAC CATGATCCAG GCTGGGGATA CAACCAAGTT TGATGTGGAG 
GTTCTCAAAC AGCTCCTGAA GCTCCTTCCA GAGAAACATG AAATTGAGAA CCTTCGGGCA 
TTCAAGGAGG AGCGAACCAA GCTGGCCAAT GCTGACCAGT TCTATGTCCT CCTGCTGGAC 
ATTCCTTGCT ACCAGCTGCG GGTGGAGTGC ATGCTGCTGT GCGAGGGCAC TGCCGTTGTG 
CTGGACATGG TGCGGCCCAA GGCCCAGCTG GTGCTCACCG CCTGTGAGAG CCTGCTCACC 
AGTCATCGGC TACCCATCTT CTGCCAGCTG ATCCTGAAAA TTGGGAACTT TCTCAACTAC 
GGCAGCCACA CGGGAGACGC AGATGGCTTC AAGATCAGCA CGTTGTTGAA ACTCACGGAG 
ACCAAGTCCC AGCAGAGCCG TGTGACACTG CTGCACCACG TGCTGGAGGA AGTGGAGAAG 
AGCCACCCTG ACCTCCTACA GCTGCCCCAG GACCTGGAGC CACCCTCCCA AGCTGCTGGG 
ATCAACTTGG AGATCATCCG CTCAGAGGCC AGTGCCAACC TGAAGAAGCT TCTAGAAACA 
GAGCAGAAGG TGTCCACCTC GGTCCCTGAG GTGCAAGAGC AGTATTCCAA GCGCCTGCAG 
GCCAGCATTG AGGCCTCTCA GGCACTGGAC AAGGTGTTTG AGGCCATTGA GCGGAAGAAG 
CTGCAGTTGG CTGATTACCT GTGTGAAGAC CCCCAGCAGC TGTCCCTGGA GGACACGTTC 
AGTACCATGA AGACCTTCCG TGACCTCTTT ACCCGTGCAC TGAAGGAGAA TAAGGACCGG 
AAGGAGCAGA TGGTAAAGGC AGAGAGGAGA AAACAGCAGT TGGCAGAGGA GGAGGCACGG 
AGGCCAAGGG GCGAGGACGG GAAGCCTGTC AGGAAGGGGC CGGGAAGGCA GGAAGAGGTG 
TGTGTCATCG ATGCCCTGCT GGCCGACATC AGGAAGGGCT TTCAGCTGCG GAAGACAGCC 
CGTGGTCGAG GCGATACTGA GGCGGGTAGC AAAGTGACCC CGGCAGACCC CCTGAAGCCC 
ACAGAGCCTG CAGCTGACAA CGATCCTAGA CAAGGCCCAG CGTGTGGCAC AAGTCACCCT 
GCCACAGAGC CAGGCCTGGA TGCCACAGCA GCTAGTGAGC CACAGGGCTG GGACCTTGTG 
GATGCCATGA CCCCCAGTCC TCTGCCCTCG GAGGAGGGTG GTCCCCCAGC CCTGGAGAGG 
CGTTCCTCCT GGTATGTTGA TGCCAGCGAC TTCCTGGTCC CCGAGGACAC CCTGAATGCC 
CAACCCTCCA CGGGGACCTG GCCAGTGACT CTGGGAGATG ATCAGTCCCT GAAGCCTCTT 
GAGTTCTCCA GCAATAAGCC CCCTGGGGCC GAGAACTCAC ACCAGGATGC TGCGGACCCT 
GAAGTCTTGT GGGGCATCCA TCAGGCCAAG GCAGACAGTA CAAGTGAGGG ACTAGAGGGC 
ACACCTGAGT ACAGCCGCGG CGCCCACCTG CCCGACACAG GCCCTGTCAG GGATGAGGAT 
GAGGACGACA CAGCCCCAGA ATCTGCGCTG GACACGTCTC TGGACAAGTC CCTCTCTGAG 
GATGCAGTGA CAGATTCCTC AGGGTCTGGC ACCCTCCCCA GGGACCGAGG GCGAGCCTCA 
AAAGGGGCAA GCAGGGGAAG GAAGAAGCGT CCCACAAGGA ACCAGGAAGG GCCCAGGACC 
TCTCCCTACC ATCCAGCTGG TCCCTCCTTT CTCCCTGGGG GCGCTCCAGG CTCAACCAAC 
ATCCAGGAGA GAGGAAGTTG CCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017650661.1
CDS	133..3699
Translation	MSVKEGAQRKWAALKEKLGPQDSDPTEANLESAEPELCIRLLQMPSVVNYSGLRKRLESSDGGWMVQFLEQSGLDLLLEALARLSGRGVARISDALLQLTCISCVRAVMNSQQGIEYILSNQGYVRQLSQALDTSNVMVKKQVFELLAALCIYSPEGHALTLDALDHYKTVCSQQYRFSVIMNELSDSDNVPYVVTLLSVINAIILGPEDLRTRAQLRSEFIGLQLLDILTRLRDLEDADLLIQLEAFEEAKAEDEEELQRVCDGVNMNSHQEVFASLFHKVSCSPASAQLLSVLQGLLHLEPAGRSGQLLWEALENLVNRAVLLASDAQACTLEEVVERLLSIKGRPRPSPLDKAHKGVQANLGQSRRDKSPQNTTTPMAKMEGQQPIVASACECVVSVQSSSGDTAPQPLVLEQRVPTPPLPSPPLPGMGCPPPPPPPLPGIGFGPPPPPPLPGMSIPSMVGGVEEIIVAQVDHSLGSAWVPSHRRVNPPTLRMKKLNWQKLPSNVAQERSSMWAALSSPCTEGVEPDFSSIEQLFSFPTAKPKEPSAAPARKEPKEVTFLDSKKSLNLNIFLKQFKCSNEEVTTMIQAGDTTKFDVEVLKQLLKLLPEKHEIENLRAFKEERTKLANADQFYVLLLDIPCYQLRVECMLLCEGTAVVLDMVRPKAQLVLTACESLLTSHRLPIFCQLILKIGNFLNYGSHTGDADGFKISTLLKLTETKSQQSRVTLLHHVLEEVEKSHPDLLQLPQDLEPPSQAAGINLEIIRSEASANLKKLLETEQKVSTSVPEVQEQYSKRLQASIEASQALDKVFEAIERKKLQLADYLCEDPQQLSLEDTFSTMKTFRDLFTRALKENKDRKEQMVKAERRKQQLAEEEARRPRGEDGKPVRKGPGRQEEVCVIDALLADIRKGFQLRKTARGRGDTEAGSKVTPADPLKPTEPAADNDPRQGPACGTSHPATEPGLDATAASEPQGWDLVDAMTPSPLPSEEGGPPALERRSSWYVDASDFLVPEDTLNAQPSTGTWPVTLGDDQSLKPLEFSSNKPPGAENSHQDAADPEVLWGIHQAKADSTSEGLEGTPEYSRGAHLPDTGPVRDEDEDDTAPESALDTSLDKSLSEDAVTDSSGSGTLPRDRGRASKGASRGRKKRPTRNQEGPRTSPYHPAGPSFLPGGAPGSTNIQERGSCP

Target ORF information:

RefSeq Version	XM_017795172.1
Organism	Nannospalax galili(Upper Galilee mountains blind mole rat)
Definition	Nannospalax galili inverted formin, FH2 and WH2 domain containing (Inf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017795172.1

ORF Insert Sequence:

ATGTCGGTGA AGGAGGGTGC ACAGCGCAAG TGGGCGGCGC TGAAGGAGAA GCTGGGGCCT 
CAGGACTCTG ACCCCACTGA AGCCAACCTG GAGAGCGCAG AGCCAGAGCT GTGCATCCGG 
CTGCTGCAGA TGCCCTCTGT GGTCAACTAC TCTGGCCTGC GAAAGCGCCT GGAGAGCAGT 
GATGGTGGCT GGATGGTGCA GTTCCTGGAG CAGAGTGGCC TGGACCTGCT GCTAGAGGCA 
CTGGCGCGGC TGTCCGGGCG TGGTGTGGCC CGCATCTCGG ATGCCCTGCT ACAGCTTACC 
TGTATCAGCT GCGTGCGTGC CGTCATGAAC TCACAGCAGG GCATAGAGTA CATTCTCAGC 
AACCAGGGCT ATGTGCGCCA GCTCTCCCAG GCTCTGGACA CGTCCAATGT GATGGTCAAG 
AAGCAGGTGT TTGAGCTGCT GGCTGCCCTG TGCATCTATT CACCTGAGGG CCACGCACTG 
ACACTAGATG CCCTGGACCA TTACAAGACG GTGTGCAGCC AGCAGTACCG CTTCAGCGTC 
ATCATGAATG AGCTCTCCGA CAGCGACAAC GTGCCCTATG TGGTCACCCT GCTCAGCGTG 
ATCAATGCCA TCATCCTGGG CCCCGAGGAT CTCCGCACTC GTGCCCAGCT TCGGAGTGAA 
TTCATTGGGC TCCAGCTGCT GGACATTCTG ACCCGGCTGC GGGACCTAGA GGATGCCGAC 
CTGCTGATCC AGCTAGAGGC CTTTGAGGAG GCCAAAGCTG AAGATGAGGA GGAGCTGCAA 
CGTGTCTGTG ATGGTGTCAA CATGAACAGC CACCAGGAAG TCTTCGCCTC TCTGTTCCAC 
AAGGTGAGCT GTTCCCCAGC ATCAGCCCAG CTGCTGTCCG TGTTACAAGG CCTCTTACAC 
CTGGAGCCTG CTGGCCGCTC GGGCCAGCTG CTCTGGGAGG CCCTAGAGAA CCTGGTGAAC 
CGGGCCGTGC TCCTGGCCAG TGATGCACAG GCTTGTACCC TGGAGGAAGT GGTGGAGCGA 
CTGCTGTCCA TCAAAGGGCG GCCCCGACCA AGCCCCCTGG ACAAGGCCCA CAAGGGCGTT 
CAGGCCAACT TGGGCCAAAG TCGGAGGGAC AAGTCCCCCC AAAACACTAC TACCCCCATG 
GCCAAAATGG AGGGCCAACA ACCAATTGTG GCTTCTGCCT GCGAGTGTGT GGTCAGCGTC 
CAGAGCTCCA GTGGTGACAC AGCCCCACAG CCATTAGTCC TTGAGCAGAG GGTGCCTACC 
CCACCTCTGC CCAGCCCCCC ACTCCCAGGC ATGGGGTGCC CACCTCCTCC CCCTCCCCCA 
CTACCTGGAA TAGGCTTCGG CCCCCCTCCA CCTCCTCCCC TGCCTGGTAT GTCCATCCCC 
TCCATGGTGG GTGGCGTGGA GGAGATCATT GTGGCCCAGG TGGACCACAG CTTGGGCTCA 
GCCTGGGTCC CCAGTCACCG CCGGGTGAAT CCACCCACAC TGCGCATGAA GAAGTTGAAC 
TGGCAAAAGC TGCCATCCAA CGTGGCACAA GAGCGCAGCT CTATGTGGGC AGCTCTGAGC 
AGCCCCTGCA CGGAGGGGGT GGAACCTGAC TTCTCCAGCA TCGAACAGCT GTTCTCCTTC 
CCCACCGCCA AGCCCAAGGA GCCCTCTGCT GCCCCAGCGA GGAAGGAGCC GAAGGAGGTC 
ACGTTTTTGG ACTCCAAGAA GAGCCTGAAC CTCAACATCT TCCTGAAGCA ATTTAAATGC 
TCCAATGAGG AGGTCACCAC CATGATCCAG GCTGGGGATA CAACCAAGTT TGATGTGGAG 
GTTCTCAAAC AGCTCCTGAA GCTCCTTCCA GAGAAACATG AAATTGAGAA CCTTCGGGCA 
TTCAAGGAGG AGCGAACCAA GCTGGCCAAT GCTGACCAGT TCTATGTCCT CCTGCTGGAC 
ATTCCTTGCT ACCAGCTGCG GGTGGAGTGC ATGCTGCTGT GCGAGGGCAC TGCCGTTGTG 
CTGGACATGG TGCGGCCCAA GGCCCAGCTG GTGCTCACCG CCTGTGAGAG CCTGCTCACC 
AGTCATCGGC TACCCATCTT CTGCCAGCTG ATCCTGAAAA TTGGGAACTT TCTCAACTAC 
GGCAGCCACA CGGGAGACGC AGATGGCTTC AAGATCAGCA CGTTGTTGAA ACTCACGGAG 
ACCAAGTCCC AGCAGAGCCG TGTGACACTG CTGCACCACG TGCTGGAGGA AGTGGAGAAG 
AGCCACCCTG ACCTCCTACA GCTGCCCCAG GACCTGGAGC CACCCTCCCA AGCTGCTGGG 
ATCAACTTGG AGATCATCCG CTCAGAGGCC AGTGCCAACC TGAAGAAGCT TCTAGAAACA 
GAGCAGAAGG TGTCCACCTC GGTCCCTGAG GTGCAAGAGC AGTATTCCAA GCGCCTGCAG 
GCCAGCATTG AGGCCTCTCA GGCACTGGAC AAGGTGTTTG AGGCCATTGA GCGGAAGAAG 
CTGCAGTTGG CTGATTACCT GTGTGAAGAC CCCCAGCAGC TGTCCCTGGA GGACACGTTC 
AGTACCATGA AGACCTTCCG TGACCTCTTT ACCCGTGCAC TGAAGGAGAA TAAGGACCGG 
AAGGAGCAGA TGGTAAAGGC AGAGAGGAGA AAACAGCAGT TGGCAGAGGA GGAGGCACGG 
AGGCCAAGGG GCGAGGACGG GAAGCCTGTC AGGAAGGGGC CGGGAAGGCA GGAAGAGGTG 
TGTGTCATCG ATGCCCTGCT GGCCGACATC AGGAAGGGCT TTCAGCTGCG GAAGACAGCC 
CGTGGTCGAG GCGATACTGA GGCGGGTAGC AAAGTGACCC CGGCAGACCC CCTGAAGCCC 
ACAGAGCCTG CAGCTGACAA CGATCCTAGA CAAGGCCCAG CGTGTGGCAC AAGTCACCCT 
GCCACAGAGC CAGGCCTGGA TGCCACAGCA GCTAGTGAGC CACAGGGCTG GGACCTTGTG 
GATGCCATGA CCCCCAGTCC TCTGCCCTCG GAGGAGGGTG GTCCCCCAGC CCTGGAGAGG 
CGTTCCTCCT GGTATGTTGA TGCCAGCGAC TTCCTGGTCC CCGAGGACAC CCTGAATGCC 
CAACCCTCCA CGGGGACCTG GCCAGTGACT CTGGGAGATG ATCAGTCCCT GAAGCCTCTT 
GAGTTCTCCA GCAATAAGCC CCCTGGGGCC GAGAACTCAC ACCAGGATGC TGCGGACCCT 
GAAGTCTTGT GGGGCATCCA TCAGGCCAAG GCAGACAGTA CAAGTGAGGG ACTAGAGGGC 
ACACCTGAGT ACAGCCGCGG CGCCCACCTG CCCGACACAG GCCCTGTCAG GGATGAGGAT 
GAGGACGACA CAGCCCCAGA ATCTGCGCTG GACACGTCTC TGGACAAGTC CCTCTCTGAG 
GATGCAGTGA CAGATTCCTC AGGGTCTGGC ACCCTCCCCA GGGACCGAGG GCGAGCCTCA 
AAAGGGGCAA GCAGGGGAAG GAAGAAGCGT CCCACAAGGA ACCAGGAAGG GCCCAGGACC 
TCTCCCTACC ATCCAGCTGG TCCCTCCTTT CTCCCTGGGG GCGCTCCAGG CTCAACCAAC 
ATCCAGGAGA GAGGAAGTTG CCCCTGA