Ropn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ropn1
Entrez Gene ID	76378
Full Name	ropporin, rhophilin associated protein 1
Synonyms	1700008N21Rik,ODF6,RHPNAP1,Rppn,ropporin
General protein information	Preferred Names ropporin, rhophilin associated protein 1 Names ropporin-1 rhophilin-associated 'oppo' protein rhophilin-associated oppo protein rhophilin-associated protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006522698.4	XP_006522761.1	ropporin-1 isoform X1
NM_030744.2	NP_109669.1	ropporin-1
XM_006522698.2	XP_006522761.1	ropporin-1 isoform X1
XM_006522698.3	XP_006522761.1	ropporin-1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ropn1	NP_109669.1
Homo sapiens (human)	ROPN1	NP_060048.2
Pan troglodytes (chimpanzee)	ROPN1	XP_001168868.1
Rattus norvegicus (Norway rat)	Ropn1	NP_001020799.1
Canis lupus familiaris (dog)	LOC488013	XP_545136.3
Bos taurus (cattle)	ROPN1	NP_001069368.1

Gene Ontology
Bibliography

Related articles in PubMed

FSCB phosphorylation regulates mouse spermatozoa capacitation through suppressing SUMOylation of ROPN1/ROPN1L.
Zhang X, Chen M, Yu R, Liu B, Tian Z, Liu S
American journal of translational research8(6)2776-82(2016)

Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine sperm motility, phosphorylation, and fibrous sheath integrity.
Fiedler SE, Dudiki T, Vijayaraghavan S, Carr DW
Biology of reproduction88(2)41(2013 Feb)

Loss of ASP but not ROPN1 reduces mammalian ciliary motility.
Fiedler SE, Sisson JH, Wyatt TA, Pavlik JA, Gambling TM, Carson JL, Carr DW
Cytoskeleton (Hoboken, N.J.)69(1)22-32(2012 Jan)

CABYR isoforms expressed in late steps of spermiogenesis bind with AKAPs and ropporin in mouse sperm fibrous sheath.
Li YF, He W, Kim YH, Mandal A, Digilio L, Klotz K, Flickinger CJ, Herr JC
Reproductive biology and endocrinology : RB&E8101(2010 Aug)

FSCB, a novel protein kinase A-phosphorylated calcium-binding protein, is a CABYR-binding partner involved in late steps of fibrous sheath biogenesis.
Li YF, He W, Jha KN, Klotz K, Kim YH, Mandal A, Pulido S, Digilio L, Flickinger CJ, Herr JC
The Journal of biological chemistry282(47)34104-19(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in ROPN1 and ROPN1L can cause defects in fibrous sheath integrity, sperm motility, and PKA-dependent signaling processes, leading to male infertility.
Title: Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine sperm motility, phosphorylation, and fibrous sheath integrity.

CABYR expresses at the late stage of spermiogenesis and its isoforms oligomerize and bind with AKAPs and ropporin.
Title: CABYR isoforms expressed in late steps of spermiogenesis bind with AKAPs and ropporin in mouse sperm fibrous sheath.

The following Ropn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ropn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16565	NM_030744.2 Latest version!	Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu16565	XM_006522698.4 Latest version!	Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu16565	XM_006522698.2	Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu16565	XM_006522698.3	Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ropn1 ( NM_030744.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_109669.1 Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), mRNA.

Price & Availability↑

CloneID	OMu16565
Clone ID Related Accession (Same CDS sequence)	XM_006522698.4 , NM_030744.2 , XM_006522698.2 , XM_006522698.3
Accession Version	NM_030744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	ropporin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC049544.1. On Dec 20, 2003 this sequence version replaced NM_030744.1. ##Evidence-Data-START## Transcript exon combination :: BC049544.1, BU946138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCAGA CAGACAAGCA AGTATGCATC CCACCGGAGC TGCCGGAATT GCTGAAGCAG 
TTTACCAAAG ATGCCATTCG GACCCAGCCT CCTGATCTCA TACAGTGGGC AGCCGAATAT 
TTCGGAGCCA TGTCCCGTGG AGAGATTCCT CCAGTGAGAG AGAGGTCTGA ACAAATCCCT 
TTGTCCAACT GGGCTGAGCT GACACCTGAG CTGCTGAAGG TCCTGCATTC TCGGGTTGCT 
GGCAGACTGA TCATCCACGC AGATGAGCTG GCCCAGATGT GGAAGGTGCT GAATCTCCCC 
ACGGACCTGT TTAACAGTGT GATGAACGTG GGTCGCTTCA CAGAGGAGAT CGAGTGGCTG 
AAGTTTTTAG CCCTTGCTTG TAGCTCTCTT GGAGTTACCA TTGCCAAAAC TCTCAAGATA 
GTGTGTGAAG TTTTATCCTC CGACCATGAT GGAGGACCAC CCCGGATTCC TTTCAGCACC 
TTCCAGTTTC TGTACACGTA TATCGCTGAA GTGGATGGGG AAATCTCTTC ATCCCACGTT 
AGCAGGATGT TAAACTACAT TGAACAGGAA GTAATTGGTC CTGATGGCTT AATCAAGGTG 
AATGACTTTA CTCAAAACCC TAGGGTTCGG CTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_109669.1
CDS	78..716
Translation	MPQTDKQVCIPPELPELLKQFTKDAIRTQPPDLIQWAAEYFGAMSRGEIPPVRERSEQIPLSNWAELTPELLKVLHSRVAGRLIIHADELAQMWKVLNLPTDLFNSVMNVGRFTEEIEWLKFLALACSSLGVTIAKTLKIVCEVLSSDHDGGPPRIPFSTFQFLYTYIAEVDGEISSSHVSRMLNYIEQEVIGPDGLIKVNDFTQNPRVRLE

Target ORF information:

RefSeq Version	NM_030744.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030744.2

ORF Insert Sequence:

ATGCCTCAGA CAGACAAGCA AGTATGCATC CCACCGGAGC TGCCGGAATT GCTGAAGCAG 
TTTACCAAAG ATGCCATTCG GACCCAGCCT CCTGATCTCA TACAGTGGGC AGCCGAATAT 
TTCGGAGCCA TGTCCCGTGG AGAGATTCCT CCAGTGAGAG AGAGGTCTGA ACAAATCCCT 
TTGTCCAACT GGGCTGAGCT GACACCTGAG CTGCTGAAGG TCCTGCATTC TCGGGTTGCT 
GGCAGACTGA TCATCCACGC AGATGAGCTG GCCCAGATGT GGAAGGTGCT GAATCTCCCC 
ACGGACCTGT TTAACAGTGT GATGAACGTG GGTCGCTTCA CAGAGGAGAT CGAGTGGCTG 
AAGTTTTTAG CCCTTGCTTG TAGCTCTCTT GGAGTTACCA TTGCCAAAAC TCTCAAGATA 
GTGTGTGAAG TTTTATCCTC CGACCATGAT GGAGGACCAC CCCGGATTCC TTTCAGCACC 
TTCCAGTTTC TGTACACGTA TATCGCTGAA GTGGATGGGG AAATCTCTTC ATCCCACGTT 
AGCAGGATGT TAAACTACAT TGAACAGGAA GTAATTGGTC CTGATGGCTT AATCAAGGTG 
AATGACTTTA CTCAAAACCC TAGGGTTCGG CTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ropn1 ( XM_006522698.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522761.1 Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16565
Clone ID Related Accession (Same CDS sequence)	XM_006522698.4 , NM_030744.2 , XM_006522698.2 , XM_006522698.3
Accession Version	XM_006522698.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ropporin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006522698.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGA CAGACAAGCA AGTATGCATC CCACCGGAGC TGCCGGAATT GCTGAAGCAG 
TTTACCAAAG ATGCCATTCG GACCCAGCCT CCTGATCTCA TACAGTGGGC AGCCGAATAT 
TTCGGAGCCA TGTCCCGTGG AGAGATTCCT CCAGTGAGAG AGAGGTCTGA ACAAATCCCT 
TTGTCCAACT GGGCTGAGCT GACACCTGAG CTGCTGAAGG TCCTGCATTC TCGGGTTGCT 
GGCAGACTGA TCATCCACGC AGATGAGCTG GCCCAGATGT GGAAGGTGCT GAATCTCCCC 
ACGGACCTGT TTAACAGTGT GATGAACGTG GGTCGCTTCA CAGAGGAGAT CGAGTGGCTG 
AAGTTTTTAG CCCTTGCTTG TAGCTCTCTT GGAGTTACCA TTGCCAAAAC TCTCAAGATA 
GTGTGTGAAG TTTTATCCTC CGACCATGAT GGAGGACCAC CCCGGATTCC TTTCAGCACC 
TTCCAGTTTC TGTACACGTA TATCGCTGAA GTGGATGGGG AAATCTCTTC ATCCCACGTT 
AGCAGGATGT TAAACTACAT TGAACAGGAA GTAATTGGTC CTGATGGCTT AATCAAGGTG 
AATGACTTTA CTCAAAACCC TAGGGTTCGG CTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522761.1
CDS	203..841
Translation	MPQTDKQVCIPPELPELLKQFTKDAIRTQPPDLIQWAAEYFGAMSRGEIPPVRERSEQIPLSNWAELTPELLKVLHSRVAGRLIIHADELAQMWKVLNLPTDLFNSVMNVGRFTEEIEWLKFLALACSSLGVTIAKTLKIVCEVLSSDHDGGPPRIPFSTFQFLYTYIAEVDGEISSSHVSRMLNYIEQEVIGPDGLIKVNDFTQNPRVRLE

Target ORF information:

RefSeq Version	XM_006522698.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522698.4

ORF Insert Sequence:

ATGCCTCAGA CAGACAAGCA AGTATGCATC CCACCGGAGC TGCCGGAATT GCTGAAGCAG 
TTTACCAAAG ATGCCATTCG GACCCAGCCT CCTGATCTCA TACAGTGGGC AGCCGAATAT 
TTCGGAGCCA TGTCCCGTGG AGAGATTCCT CCAGTGAGAG AGAGGTCTGA ACAAATCCCT 
TTGTCCAACT GGGCTGAGCT GACACCTGAG CTGCTGAAGG TCCTGCATTC TCGGGTTGCT 
GGCAGACTGA TCATCCACGC AGATGAGCTG GCCCAGATGT GGAAGGTGCT GAATCTCCCC 
ACGGACCTGT TTAACAGTGT GATGAACGTG GGTCGCTTCA CAGAGGAGAT CGAGTGGCTG 
AAGTTTTTAG CCCTTGCTTG TAGCTCTCTT GGAGTTACCA TTGCCAAAAC TCTCAAGATA 
GTGTGTGAAG TTTTATCCTC CGACCATGAT GGAGGACCAC CCCGGATTCC TTTCAGCACC 
TTCCAGTTTC TGTACACGTA TATCGCTGAA GTGGATGGGG AAATCTCTTC ATCCCACGTT 
AGCAGGATGT TAAACTACAT TGAACAGGAA GTAATTGGTC CTGATGGCTT AATCAAGGTG 
AATGACTTTA CTCAAAACCC TAGGGTTCGG CTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ropn1 ( XM_006522698.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522761.1 Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16565
Clone ID Related Accession (Same CDS sequence)	XM_006522698.4 , NM_030744.2 , XM_006522698.2 , XM_006522698.3
Accession Version	XM_006522698.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	ropporin-1 isoform X1
Comment

ATGCCTCAGA CAGACAAGCA AGTATGCATC CCACCGGAGC TGCCGGAATT GCTGAAGCAG 
TTTACCAAAG ATGCCATTCG GACCCAGCCT CCTGATCTCA TACAGTGGGC AGCCGAATAT 
TTCGGAGCCA TGTCCCGTGG AGAGATTCCT CCAGTGAGAG AGAGGTCTGA ACAAATCCCT 
TTGTCCAACT GGGCTGAGCT GACACCTGAG CTGCTGAAGG TCCTGCATTC TCGGGTTGCT 
GGCAGACTGA TCATCCACGC AGATGAGCTG GCCCAGATGT GGAAGGTGCT GAATCTCCCC 
ACGGACCTGT TTAACAGTGT GATGAACGTG GGTCGCTTCA CAGAGGAGAT CGAGTGGCTG 
AAGTTTTTAG CCCTTGCTTG TAGCTCTCTT GGAGTTACCA TTGCCAAAAC TCTCAAGATA 
GTGTGTGAAG TTTTATCCTC CGACCATGAT GGAGGACCAC CCCGGATTCC TTTCAGCACC 
TTCCAGTTTC TGTACACGTA TATCGCTGAA GTGGATGGGG AAATCTCTTC ATCCCACGTT 
AGCAGGATGT TAAACTACAT TGAACAGGAA GTAATTGGTC CTGATGGCTT AATCAAGGTG 
AATGACTTTA CTCAAAACCC TAGGGTTCGG CTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522761.1
Translation	MPQTDKQVCIPPELPELLKQFTKDAIRTQPPDLIQWAAEYFGAMSRGEIPPVRERSEQIPLSNWAELTPELLKVLHSRVAGRLIIHADELAQMWKVLNLPTDLFNSVMNVGRFTEEIEWLKFLALACSSLGVTIAKTLKIVCEVLSSDHDGGPPRIPFSTFQFLYTYIAEVDGEISSSHVSRMLNYIEQEVIGPDGLIKVNDFTQNPRVRLE

Target ORF information:

RefSeq Version	XM_006522698.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522698.2

ORF Insert Sequence:

ATGCCTCAGA CAGACAAGCA AGTATGCATC CCACCGGAGC TGCCGGAATT GCTGAAGCAG 
TTTACCAAAG ATGCCATTCG GACCCAGCCT CCTGATCTCA TACAGTGGGC AGCCGAATAT 
TTCGGAGCCA TGTCCCGTGG AGAGATTCCT CCAGTGAGAG AGAGGTCTGA ACAAATCCCT 
TTGTCCAACT GGGCTGAGCT GACACCTGAG CTGCTGAAGG TCCTGCATTC TCGGGTTGCT 
GGCAGACTGA TCATCCACGC AGATGAGCTG GCCCAGATGT GGAAGGTGCT GAATCTCCCC 
ACGGACCTGT TTAACAGTGT GATGAACGTG GGTCGCTTCA CAGAGGAGAT CGAGTGGCTG 
AAGTTTTTAG CCCTTGCTTG TAGCTCTCTT GGAGTTACCA TTGCCAAAAC TCTCAAGATA 
GTGTGTGAAG TTTTATCCTC CGACCATGAT GGAGGACCAC CCCGGATTCC TTTCAGCACC 
TTCCAGTTTC TGTACACGTA TATCGCTGAA GTGGATGGGG AAATCTCTTC ATCCCACGTT 
AGCAGGATGT TAAACTACAT TGAACAGGAA GTAATTGGTC CTGATGGCTT AATCAAGGTG 
AATGACTTTA CTCAAAACCC TAGGGTTCGG CTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ropn1 ( XM_006522698.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006522761.1 Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16565
Clone ID Related Accession (Same CDS sequence)	XM_006522698.4 , NM_030744.2 , XM_006522698.2 , XM_006522698.3
Accession Version	XM_006522698.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ropporin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522698.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCAGA CAGACAAGCA AGTATGCATC CCACCGGAGC TGCCGGAATT GCTGAAGCAG 
TTTACCAAAG ATGCCATTCG GACCCAGCCT CCTGATCTCA TACAGTGGGC AGCCGAATAT 
TTCGGAGCCA TGTCCCGTGG AGAGATTCCT CCAGTGAGAG AGAGGTCTGA ACAAATCCCT 
TTGTCCAACT GGGCTGAGCT GACACCTGAG CTGCTGAAGG TCCTGCATTC TCGGGTTGCT 
GGCAGACTGA TCATCCACGC AGATGAGCTG GCCCAGATGT GGAAGGTGCT GAATCTCCCC 
ACGGACCTGT TTAACAGTGT GATGAACGTG GGTCGCTTCA CAGAGGAGAT CGAGTGGCTG 
AAGTTTTTAG CCCTTGCTTG TAGCTCTCTT GGAGTTACCA TTGCCAAAAC TCTCAAGATA 
GTGTGTGAAG TTTTATCCTC CGACCATGAT GGAGGACCAC CCCGGATTCC TTTCAGCACC 
TTCCAGTTTC TGTACACGTA TATCGCTGAA GTGGATGGGG AAATCTCTTC ATCCCACGTT 
AGCAGGATGT TAAACTACAT TGAACAGGAA GTAATTGGTC CTGATGGCTT AATCAAGGTG 
AATGACTTTA CTCAAAACCC TAGGGTTCGG CTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006522761.1
CDS	195..833
Translation	MPQTDKQVCIPPELPELLKQFTKDAIRTQPPDLIQWAAEYFGAMSRGEIPPVRERSEQIPLSNWAELTPELLKVLHSRVAGRLIIHADELAQMWKVLNLPTDLFNSVMNVGRFTEEIEWLKFLALACSSLGVTIAKTLKIVCEVLSSDHDGGPPRIPFSTFQFLYTYIAEVDGEISSSHVSRMLNYIEQEVIGPDGLIKVNDFTQNPRVRLE

Target ORF information:

RefSeq Version	XM_006522698.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ropporin, rhophilin associated protein 1 (Ropn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006522698.3

ORF Insert Sequence:

ATGCCTCAGA CAGACAAGCA AGTATGCATC CCACCGGAGC TGCCGGAATT GCTGAAGCAG 
TTTACCAAAG ATGCCATTCG GACCCAGCCT CCTGATCTCA TACAGTGGGC AGCCGAATAT 
TTCGGAGCCA TGTCCCGTGG AGAGATTCCT CCAGTGAGAG AGAGGTCTGA ACAAATCCCT 
TTGTCCAACT GGGCTGAGCT GACACCTGAG CTGCTGAAGG TCCTGCATTC TCGGGTTGCT 
GGCAGACTGA TCATCCACGC AGATGAGCTG GCCCAGATGT GGAAGGTGCT GAATCTCCCC 
ACGGACCTGT TTAACAGTGT GATGAACGTG GGTCGCTTCA CAGAGGAGAT CGAGTGGCTG 
AAGTTTTTAG CCCTTGCTTG TAGCTCTCTT GGAGTTACCA TTGCCAAAAC TCTCAAGATA 
GTGTGTGAAG TTTTATCCTC CGACCATGAT GGAGGACCAC CCCGGATTCC TTTCAGCACC 
TTCCAGTTTC TGTACACGTA TATCGCTGAA GTGGATGGGG AAATCTCTTC ATCCCACGTT 
AGCAGGATGT TAAACTACAT TGAACAGGAA GTAATTGGTC CTGATGGCTT AATCAAGGTG 
AATGACTTTA CTCAAAACCC TAGGGTTCGG CTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FSCB phosphorylation regulates mouse spermatozoa capacitation through suppressing SUMOylation of ROPN1/ROPN1L.
American journal of translational research8(6)2776-82(2016)
Zhang X,Chen M,Yu R,Liu B,Tian Z,Liu S

Loss of R2D2 proteins ROPN1 and ROPN1L causes defects in murine sperm motility, phosphorylation, and fibrous sheath integrity.
Biology of reproduction88(2)41(2013 Feb)
Fiedler SE,Dudiki T,Vijayaraghavan S,Carr DW

Loss of ASP but not ROPN1 reduces mammalian ciliary motility.
Cytoskeleton (Hoboken, N.J.)69(1)22-32(2012 Jan)
Fiedler SE,Sisson JH,Wyatt TA,Pavlik JA,Gambling TM,Carson JL,Carr DW

CABYR isoforms expressed in late steps of spermiogenesis bind with AKAPs and ropporin in mouse sperm fibrous sheath.
Reproductive biology and endocrinology : RB&E8101(2010 Aug)
Li YF,He W,Kim YH,Mandal A,Digilio L,Klotz K,Flickinger CJ,Herr JC

FSCB, a novel protein kinase A-phosphorylated calcium-binding protein, is a CABYR-binding partner involved in late steps of fibrous sheath biogenesis.
The Journal of biological chemistry282(47)34104-19(2007 Nov)
Li YF,He W,Jha KN,Klotz K,Kim YH,Mandal A,Pulido S,Digilio L,Flickinger CJ,Herr JC

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Ropn1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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