Lpo cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lpo
Entrez Gene ID	76113
Full Name	lactoperoxidase
Synonyms	5830499B15Rik
General protein information	Preferred Names lactoperoxidase Names lactoperoxidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_011249298.3	XP_011247600.1	lactoperoxidase isoform X1
XM_011249298.2	XP_011247600.1	lactoperoxidase isoform X1
NM_080420.2	NP_536345.2	lactoperoxidase precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	LPO	NP_006142.1
	LPO	XP_001106438.1
Pan troglodytes (chimpanzee)	LPO	XP_003315682.1
Rattus norvegicus (Norway rat)	Lpo	NP_001099299.1
Canis lupus familiaris (dog)	LPO	XP_548231.3
Bos taurus (cattle)	LPO	NP_776358.1
Mus musculus (house mouse)	Lpo	NP_536345.2

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Loss of miR-10a activates lpo and collaborates with activated Wnt signaling in inducing intestinal neoplasia in female mice.
Stadthagen G, Tehler D, H?yland-Kroghsbo NM, Wen J, Krogh A, Jensen KT, Santoni-Rugiu E, Engelholm LH, Lund AH
PLoS genetics9(10)e1003913(2013 Oct)

Expression of lactoperoxidase in differentiated mouse colon epithelial cells.
Kim BW, Esworthy RS, Hahn MA, Pfeifer GP, Chu FF
Free radical biology & medicine52(9)1569-76(2012 May)

The evolutionary conservation of the mammalian peroxidase genes.
Sakamaki K, Ueda T, Nagata S
Cytogenetic and genome research98(1)93-5(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we postulate that Lpo upregulation in the intestinal epithelium of miR-10a deficient mice together with the predominant abundance of estrogens in female animals mainly accounts for the sex-related cancer phenotype we observed
Title: Loss of miR-10a activates lpo and collaborates with activated Wnt signaling in inducing intestinal neoplasia in female mice.

methylation of the Lpo intragenic CpG island was not directly induced by inflammation, because dextran sulfate sodium-induced colitis did not increase DNA methylation in B6 DKO colon. Also, Lpo DNA methylation is not correlated with gene expression
Title: Expression of lactoperoxidase in differentiated mouse colon epithelial cells.

The following Lpo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lpo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09629	NM_080420.2 Latest version!	Mus musculus lactoperoxidase (Lpo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu09629	XM_011249298.3 Latest version!	Mus musculus lactoperoxidase (Lpo), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu09629	XM_011249298.2	Mus musculus lactoperoxidase (Lpo), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lpo ( NM_080420.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_536345.2 Mus musculus lactoperoxidase (Lpo), mRNA.

Price & Availability↑

CloneID	OMu09629
Clone ID Related Accession (Same CDS sequence)	XM_011249298.3 , XM_011249298.2 , NM_080420.2
Accession Version	NM_080420.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Mus musculus(house mouse)
Product	lactoperoxidase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL606805.21 and AL604022.21. On May 28, 2009 this sequence version replaced NM_080420.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC016212.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849389 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGTGC TTCTGCATCT CCCAGCCCTC CTGGCTTCCC TGACCTTGCT CCAGACTGCG 
GCATCTGCCA GCGATGATCC AACTGCCGAG ACTGACATCA TCCACGACAC AGTGGAAGAG 
GTCAAGGTCT GGGTCAACAA GGCCTTCCTG GATTCCCGGG ACAGGCTGAA GATGGCCATG 
ACAACCAAGA TACATAGCAC CCGTCATCTG TCAGATTATC TCAAGCATGC CAAAGGCCGG 
ACACGCACAG CCATCCGGAG TGGGCAGGTG TGGGAAGAGT CCCTAAAGAA ACTGTCTCAG 
TTTCTCACCA ATGTCACAGG TCAAGGCCTG GACTTGACTT TGCTCTCTTG GGAGGCGGGC 
TGTGATCCCC CTGCTCCAAC AATGACTTGC AACATAAGTA GCCCTTACCG GACCATCACT 
GGATACTGTA ACAACAGGAA GAATCCAGCG CTGGGATCCG CCAACAGGGC GCTGGCTCGA 
TGGCTGCCCG CAGAATACGA GGACGGGCTC TCCCTCCCTT ACGGGTGGAC GCCGGGGAAG 
ATGCGGAACG GCTTCCCTCT CCCGCAGCCC CGTGAAGTCT CTAACCAGAT TGCAGCCTAT 
CTGAATGAGG AGGACGTTCT GGACCAGAAG AGATCCATGC TCTTCATGCA GTGGGGTCAA 
ATCGTGGACC ACGACATGGA CTTTGCCCCT GAAACTGAAA TGGGGAGTGA TACCTACACC 
AAAGCCCAGT GTGACGAACA CTGTATCCAG GGAGACAACT GCTTCCCCAT CATGTTCCCA 
CCTGGTGACC CTAAATTAAA GACTCAGGGG AAGTGCATGC CTTTCTTCAG AGCTGGATTT 
GTCTGCCCCA CACCACCCTA TAAATCCCTG GCCCGAGAGC AGATCAATGC TCTAACCTCT 
TTCCTGGATG CTAGCCTAGT GTATAGCCCT GAGCCAAGCC TGGCAAATCG ACTTAGAAAC 
CTCAGCAGCC CCTTGGGCCT CATGGCTGTC AATGAAGAAG TCTCAGACAA TGGGCGGCCC 
TTCCCGCCTT TCGTCAAAAT GAAACCCAGC CCCTGCGAGG TCATCAATGC CACAGCTGGT 
GTGCCCTGCT TCTTGGCAGG GGACTCCCGA GCCTCAGAGC AGATCTTGTT GGCCACATCC 
CACACACTCT TTATCCGGGA GCACAACCGG TTGGCCACAG AACTAAGCAG ACTCAACCCG 
CATTGGGACA GAGAGACGCT CTATCAGGAA GCAAGGAAAA TCATGGGAGC CTTCATACAG 
ATTACCACCT TTAGGGACTA CCTACCCATC CTACTGGGTG ATGAGATGCA GAAGTGGATT 
CCCCCATACC AAGGCTACAA TGAGTCTGTA GACCCCCGAA TTTCCAATGT CTTCACCTTT 
GCTTTACGCT TCGGCCACTT GGAGATCCCC TCTACTGTGT ACCGCCTAGA TGAGAATTAT 
CAGCCGTGGG GTTCAGAATC TGAGCTACCC CTGCACACTG TCTTTTTCAA CACCTGGAGG 
CTGGTCAAGG ATGGTGGAAT TGATCCTCTG GTTCGAGGTC TGTTGGCCAA GAATGCCAAG 
TTGATGCATC AGAACAAAAT GATGACAGGC GAGCTTCGAA ACAAGCTGTT CCAACCGAAT 
CACACCATCC ACGGCTTCGA CCTGGCTTCC ATCAACATAC AGCGGAGCAG AGACCACGGG 
CAGCCTGGGT ACAATTCCTG GAGAGCTTTC TGTGGCCTGT CCCAACCAAA AACACTAGAG 
GAGCTGAGTG CTGTCATGAA AAACGAGGTG CTGGCCAAGA AGCTGATGGA CCTCTATGGA 
ACCCCGAGCA ACATTGATAT CTGGCTAGGG GCCGTCGCTG AACCCCTGGT CCACAGGGGT 
CGGGTGGGAC CTCTCTTGAC CTGTCTCCTG GGCCAGCAGT TCCAGCGGAT CCGAGATGGA 
GACAGGTTCT GGTGGGAGAA CCCCGGGGTC TTCACAGAGA AGCAACGGGA GTCTCTACAG 
AAGATGTCCT TCTCCCGCCT GGTCTGTGAC AACACTGGCA TCGACAAGGT CCCACTGAAC 
CCGTTCCAGG CTAATGCCTA CCCCCATGGC TTTGTGGACT GCTCTTCAAT TGATAAGCTG 
GACCTCTCAC CCTGGGCCTC AGTGAAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_536345.2
CDS	376..2508
Translation	MKVLLHLPALLASLTLLQTAASASDDPTAETDIIHDTVEEVKVWVNKAFLDSRDRLKMAMTTKIHSTRHLSDYLKHAKGRTRTAIRSGQVWEESLKKLSQFLTNVTGQGLDLTLLSWEAGCDPPAPTMTCNISSPYRTITGYCNNRKNPALGSANRALARWLPAEYEDGLSLPYGWTPGKMRNGFPLPQPREVSNQIAAYLNEEDVLDQKRSMLFMQWGQIVDHDMDFAPETEMGSDTYTKAQCDEHCIQGDNCFPIMFPPGDPKLKTQGKCMPFFRAGFVCPTPPYKSLAREQINALTSFLDASLVYSPEPSLANRLRNLSSPLGLMAVNEEVSDNGRPFPPFVKMKPSPCEVINATAGVPCFLAGDSRASEQILLATSHTLFIREHNRLATELSRLNPHWDRETLYQEARKIMGAFIQITTFRDYLPILLGDEMQKWIPPYQGYNESVDPRISNVFTFALRFGHLEIPSTVYRLDENYQPWGSESELPLHTVFFNTWRLVKDGGIDPLVRGLLAKNAKLMHQNKMMTGELRNKLFQPNHTIHGFDLASINIQRSRDHGQPGYNSWRAFCGLSQPKTLEELSAVMKNEVLAKKLMDLYGTPSNIDIWLGAVAEPLVHRGRVGPLLTCLLGQQFQRIRDGDRFWWENPGVFTEKQRESLQKMSFSRLVCDNTGIDKVPLNPFQANAYPHGFVDCSSIDKLDLSPWASVKE

Target ORF information:

RefSeq Version	NM_080420.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lactoperoxidase (Lpo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080420.2

ORF Insert Sequence:

ATGAAAGTGC TTCTGCATCT CCCAGCCCTC CTGGCTTCCC TGACCTTGCT CCAGACTGCG 
GCATCTGCCA GCGATGATCC AACTGCCGAG ACTGACATCA TCCACGACAC AGTGGAAGAG 
GTCAAGGTCT GGGTCAACAA GGCCTTCCTG GATTCCCGGG ACAGGCTGAA GATGGCCATG 
ACAACCAAGA TACATAGCAC CCGTCATCTG TCAGATTATC TCAAGCATGC CAAAGGCCGG 
ACACGCACAG CCATCCGGAG TGGGCAGGTG TGGGAAGAGT CCCTAAAGAA ACTGTCTCAG 
TTTCTCACCA ATGTCACAGG TCAAGGCCTG GACTTGACTT TGCTCTCTTG GGAGGCGGGC 
TGTGATCCCC CTGCTCCAAC AATGACTTGC AACATAAGTA GCCCTTACCG GACCATCACT 
GGATACTGTA ACAACAGGAA GAATCCAGCG CTGGGATCCG CCAACAGGGC GCTGGCTCGA 
TGGCTGCCCG CAGAATACGA GGACGGGCTC TCCCTCCCTT ACGGGTGGAC GCCGGGGAAG 
ATGCGGAACG GCTTCCCTCT CCCGCAGCCC CGTGAAGTCT CTAACCAGAT TGCAGCCTAT 
CTGAATGAGG AGGACGTTCT GGACCAGAAG AGATCCATGC TCTTCATGCA GTGGGGTCAA 
ATCGTGGACC ACGACATGGA CTTTGCCCCT GAAACTGAAA TGGGGAGTGA TACCTACACC 
AAAGCCCAGT GTGACGAACA CTGTATCCAG GGAGACAACT GCTTCCCCAT CATGTTCCCA 
CCTGGTGACC CTAAATTAAA GACTCAGGGG AAGTGCATGC CTTTCTTCAG AGCTGGATTT 
GTCTGCCCCA CACCACCCTA TAAATCCCTG GCCCGAGAGC AGATCAATGC TCTAACCTCT 
TTCCTGGATG CTAGCCTAGT GTATAGCCCT GAGCCAAGCC TGGCAAATCG ACTTAGAAAC 
CTCAGCAGCC CCTTGGGCCT CATGGCTGTC AATGAAGAAG TCTCAGACAA TGGGCGGCCC 
TTCCCGCCTT TCGTCAAAAT GAAACCCAGC CCCTGCGAGG TCATCAATGC CACAGCTGGT 
GTGCCCTGCT TCTTGGCAGG GGACTCCCGA GCCTCAGAGC AGATCTTGTT GGCCACATCC 
CACACACTCT TTATCCGGGA GCACAACCGG TTGGCCACAG AACTAAGCAG ACTCAACCCG 
CATTGGGACA GAGAGACGCT CTATCAGGAA GCAAGGAAAA TCATGGGAGC CTTCATACAG 
ATTACCACCT TTAGGGACTA CCTACCCATC CTACTGGGTG ATGAGATGCA GAAGTGGATT 
CCCCCATACC AAGGCTACAA TGAGTCTGTA GACCCCCGAA TTTCCAATGT CTTCACCTTT 
GCTTTACGCT TCGGCCACTT GGAGATCCCC TCTACTGTGT ACCGCCTAGA TGAGAATTAT 
CAGCCGTGGG GTTCAGAATC TGAGCTACCC CTGCACACTG TCTTTTTCAA CACCTGGAGG 
CTGGTCAAGG ATGGTGGAAT TGATCCTCTG GTTCGAGGTC TGTTGGCCAA GAATGCCAAG 
TTGATGCATC AGAACAAAAT GATGACAGGC GAGCTTCGAA ACAAGCTGTT CCAACCGAAT 
CACACCATCC ACGGCTTCGA CCTGGCTTCC ATCAACATAC AGCGGAGCAG AGACCACGGG 
CAGCCTGGGT ACAATTCCTG GAGAGCTTTC TGTGGCCTGT CCCAACCAAA AACACTAGAG 
GAGCTGAGTG CTGTCATGAA AAACGAGGTG CTGGCCAAGA AGCTGATGGA CCTCTATGGA 
ACCCCGAGCA ACATTGATAT CTGGCTAGGG GCCGTCGCTG AACCCCTGGT CCACAGGGGT 
CGGGTGGGAC CTCTCTTGAC CTGTCTCCTG GGCCAGCAGT TCCAGCGGAT CCGAGATGGA 
GACAGGTTCT GGTGGGAGAA CCCCGGGGTC TTCACAGAGA AGCAACGGGA GTCTCTACAG 
AAGATGTCCT TCTCCCGCCT GGTCTGTGAC AACACTGGCA TCGACAAGGT CCCACTGAAC 
CCGTTCCAGG CTAATGCCTA CCCCCATGGC TTTGTGGACT GCTCTTCAAT TGATAAGCTG 
GACCTCTCAC CCTGGGCCTC AGTGAAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lpo ( XM_011249298.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247600.1 Mus musculus lactoperoxidase (Lpo), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu09629
Clone ID Related Accession (Same CDS sequence)	XM_011249298.3 , XM_011249298.2 , NM_080420.2
Accession Version	XM_011249298.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lactoperoxidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249298.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTGC TTCTGCATCT CCCAGCCCTC CTGGCTTCCC TGACCTTGCT CCAGACTGCG 
GCATCTGCCA GCGATGATCC AACTGCCGAG ACTGACATCA TCCACGACAC AGTGGAAGAG 
GTCAAGGTCT GGGTCAACAA GGCCTTCCTG GATTCCCGGG ACAGGCTGAA GATGGCCATG 
ACAACCAAGA TACATAGCAC CCGTCATCTG TCAGATTATC TCAAGCATGC CAAAGGCCGG 
ACACGCACAG CCATCCGGAG TGGGCAGGTG TGGGAAGAGT CCCTAAAGAA ACTGTCTCAG 
TTTCTCACCA ATGTCACAGG TCAAGGCCTG GACTTGACTT TGCTCTCTTG GGAGGCGGGC 
TGTGATCCCC CTGCTCCAAC AATGACTTGC AACATAAGTA GCCCTTACCG GACCATCACT 
GGATACTGTA ACAACAGGAA GAATCCAGCG CTGGGATCCG CCAACAGGGC GCTGGCTCGA 
TGGCTGCCCG CAGAATACGA GGACGGGCTC TCCCTCCCTT ACGGGTGGAC GCCGGGGAAG 
ATGCGGAACG GCTTCCCTCT CCCGCAGCCC CGTGAAGTCT CTAACCAGAT TGCAGCCTAT 
CTGAATGAGG AGGACGTTCT GGACCAGAAG AGATCCATGC TCTTCATGCA GTGGGGTCAA 
ATCGTGGACC ACGACATGGA CTTTGCCCCT GAAACTGAAA TGGGGAGTGA TACCTACACC 
AAAGCCCAGT GTGACGAACA CTGTATCCAG GGAGACAACT GCTTCCCCAT CATGTTCCCA 
CCTGGTGACC CTAAATTAAA GACTCAGGGG AAGTGCATGC CTTTCTTCAG AGCTGGATTT 
GTCTGCCCCA CACCACCCTA TAAATCCCTG GCCCGAGAGC AGATCAATGC TCTAACCTCT 
TTCCTGGATG CTAGCCTAGT GTATAGCCCT GAGCCAAGCC TGGCAAATCG ACTTAGAAAC 
CTCAGCAGCC CCTTGGGCCT CATGGCTGTC AATGAAGAAG TCTCAGACAA TGGGCGGCCC 
TTCCCGCCTT TCGTCAAAAT GAAACCCAGC CCCTGCGAGG TCATCAATGC CACAGCTGGT 
GTGCCCTGCT TCTTGGCAGG GGACTCCCGA GCCTCAGAGC AGATCTTGTT GGCCACATCC 
CACACACTCT TTATCCGGGA GCACAACCGG TTGGCCACAG AACTAAGCAG ACTCAACCCG 
CATTGGGACA GAGAGACGCT CTATCAGGAA GCAAGGAAAA TCATGGGAGC CTTCATACAG 
ATTACCACCT TTAGGGACTA CCTACCCATC CTACTGGGTG ATGAGATGCA GAAGTGGATT 
CCCCCATACC AAGGCTACAA TGAGTCTGTA GACCCCCGAA TTTCCAATGT CTTCACCTTT 
GCTTTACGCT TCGGCCACTT GGAGATCCCC TCTACTGTGT ACCGCCTAGA TGAGAATTAT 
CAGCCGTGGG GTTCAGAATC TGAGCTACCC CTGCACACTG TCTTTTTCAA CACCTGGAGG 
CTGGTCAAGG ATGGTGGAAT TGATCCTCTG GTTCGAGGTC TGTTGGCCAA GAATGCCAAG 
TTGATGCATC AGAACAAAAT GATGACAGGC GAGCTTCGAA ACAAGCTGTT CCAACCGAAT 
CACACCATCC ACGGCTTCGA CCTGGCTTCC ATCAACATAC AGCGGAGCAG AGACCACGGG 
CAGCCTGGGT ACAATTCCTG GAGAGCTTTC TGTGGCCTGT CCCAACCAAA AACACTAGAG 
GAGCTGAGTG CTGTCATGAA AAACGAGGTG CTGGCCAAGA AGCTGATGGA CCTCTATGGA 
ACCCCGAGCA ACATTGATAT CTGGCTAGGG GCCGTCGCTG AACCCCTGGT CCACAGGGGT 
CGGGTGGGAC CTCTCTTGAC CTGTCTCCTG GGCCAGCAGT TCCAGCGGAT CCGAGATGGA 
GACAGGTTCT GGTGGGAGAA CCCCGGGGTC TTCACAGAGA AGCAACGGGA GTCTCTACAG 
AAGATGTCCT TCTCCCGCCT GGTCTGTGAC AACACTGGCA TCGACAAGGT CCCACTGAAC 
CCGTTCCAGG CTAATGCCTA CCCCCATGGC TTTGTGGACT GCTCTTCAAT TGATAAGCTG 
GACCTCTCAC CCTGGGCCTC AGTGAAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247600.1
CDS	413..2545
Translation	MKVLLHLPALLASLTLLQTAASASDDPTAETDIIHDTVEEVKVWVNKAFLDSRDRLKMAMTTKIHSTRHLSDYLKHAKGRTRTAIRSGQVWEESLKKLSQFLTNVTGQGLDLTLLSWEAGCDPPAPTMTCNISSPYRTITGYCNNRKNPALGSANRALARWLPAEYEDGLSLPYGWTPGKMRNGFPLPQPREVSNQIAAYLNEEDVLDQKRSMLFMQWGQIVDHDMDFAPETEMGSDTYTKAQCDEHCIQGDNCFPIMFPPGDPKLKTQGKCMPFFRAGFVCPTPPYKSLAREQINALTSFLDASLVYSPEPSLANRLRNLSSPLGLMAVNEEVSDNGRPFPPFVKMKPSPCEVINATAGVPCFLAGDSRASEQILLATSHTLFIREHNRLATELSRLNPHWDRETLYQEARKIMGAFIQITTFRDYLPILLGDEMQKWIPPYQGYNESVDPRISNVFTFALRFGHLEIPSTVYRLDENYQPWGSESELPLHTVFFNTWRLVKDGGIDPLVRGLLAKNAKLMHQNKMMTGELRNKLFQPNHTIHGFDLASINIQRSRDHGQPGYNSWRAFCGLSQPKTLEELSAVMKNEVLAKKLMDLYGTPSNIDIWLGAVAEPLVHRGRVGPLLTCLLGQQFQRIRDGDRFWWENPGVFTEKQRESLQKMSFSRLVCDNTGIDKVPLNPFQANAYPHGFVDCSSIDKLDLSPWASVKE

Target ORF information:

RefSeq Version	XM_011249298.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lactoperoxidase (Lpo), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249298.2

ORF Insert Sequence:

ATGAAAGTGC TTCTGCATCT CCCAGCCCTC CTGGCTTCCC TGACCTTGCT CCAGACTGCG 
GCATCTGCCA GCGATGATCC AACTGCCGAG ACTGACATCA TCCACGACAC AGTGGAAGAG 
GTCAAGGTCT GGGTCAACAA GGCCTTCCTG GATTCCCGGG ACAGGCTGAA GATGGCCATG 
ACAACCAAGA TACATAGCAC CCGTCATCTG TCAGATTATC TCAAGCATGC CAAAGGCCGG 
ACACGCACAG CCATCCGGAG TGGGCAGGTG TGGGAAGAGT CCCTAAAGAA ACTGTCTCAG 
TTTCTCACCA ATGTCACAGG TCAAGGCCTG GACTTGACTT TGCTCTCTTG GGAGGCGGGC 
TGTGATCCCC CTGCTCCAAC AATGACTTGC AACATAAGTA GCCCTTACCG GACCATCACT 
GGATACTGTA ACAACAGGAA GAATCCAGCG CTGGGATCCG CCAACAGGGC GCTGGCTCGA 
TGGCTGCCCG CAGAATACGA GGACGGGCTC TCCCTCCCTT ACGGGTGGAC GCCGGGGAAG 
ATGCGGAACG GCTTCCCTCT CCCGCAGCCC CGTGAAGTCT CTAACCAGAT TGCAGCCTAT 
CTGAATGAGG AGGACGTTCT GGACCAGAAG AGATCCATGC TCTTCATGCA GTGGGGTCAA 
ATCGTGGACC ACGACATGGA CTTTGCCCCT GAAACTGAAA TGGGGAGTGA TACCTACACC 
AAAGCCCAGT GTGACGAACA CTGTATCCAG GGAGACAACT GCTTCCCCAT CATGTTCCCA 
CCTGGTGACC CTAAATTAAA GACTCAGGGG AAGTGCATGC CTTTCTTCAG AGCTGGATTT 
GTCTGCCCCA CACCACCCTA TAAATCCCTG GCCCGAGAGC AGATCAATGC TCTAACCTCT 
TTCCTGGATG CTAGCCTAGT GTATAGCCCT GAGCCAAGCC TGGCAAATCG ACTTAGAAAC 
CTCAGCAGCC CCTTGGGCCT CATGGCTGTC AATGAAGAAG TCTCAGACAA TGGGCGGCCC 
TTCCCGCCTT TCGTCAAAAT GAAACCCAGC CCCTGCGAGG TCATCAATGC CACAGCTGGT 
GTGCCCTGCT TCTTGGCAGG GGACTCCCGA GCCTCAGAGC AGATCTTGTT GGCCACATCC 
CACACACTCT TTATCCGGGA GCACAACCGG TTGGCCACAG AACTAAGCAG ACTCAACCCG 
CATTGGGACA GAGAGACGCT CTATCAGGAA GCAAGGAAAA TCATGGGAGC CTTCATACAG 
ATTACCACCT TTAGGGACTA CCTACCCATC CTACTGGGTG ATGAGATGCA GAAGTGGATT 
CCCCCATACC AAGGCTACAA TGAGTCTGTA GACCCCCGAA TTTCCAATGT CTTCACCTTT 
GCTTTACGCT TCGGCCACTT GGAGATCCCC TCTACTGTGT ACCGCCTAGA TGAGAATTAT 
CAGCCGTGGG GTTCAGAATC TGAGCTACCC CTGCACACTG TCTTTTTCAA CACCTGGAGG 
CTGGTCAAGG ATGGTGGAAT TGATCCTCTG GTTCGAGGTC TGTTGGCCAA GAATGCCAAG 
TTGATGCATC AGAACAAAAT GATGACAGGC GAGCTTCGAA ACAAGCTGTT CCAACCGAAT 
CACACCATCC ACGGCTTCGA CCTGGCTTCC ATCAACATAC AGCGGAGCAG AGACCACGGG 
CAGCCTGGGT ACAATTCCTG GAGAGCTTTC TGTGGCCTGT CCCAACCAAA AACACTAGAG 
GAGCTGAGTG CTGTCATGAA AAACGAGGTG CTGGCCAAGA AGCTGATGGA CCTCTATGGA 
ACCCCGAGCA ACATTGATAT CTGGCTAGGG GCCGTCGCTG AACCCCTGGT CCACAGGGGT 
CGGGTGGGAC CTCTCTTGAC CTGTCTCCTG GGCCAGCAGT TCCAGCGGAT CCGAGATGGA 
GACAGGTTCT GGTGGGAGAA CCCCGGGGTC TTCACAGAGA AGCAACGGGA GTCTCTACAG 
AAGATGTCCT TCTCCCGCCT GGTCTGTGAC AACACTGGCA TCGACAAGGT CCCACTGAAC 
CCGTTCCAGG CTAATGCCTA CCCCCATGGC TTTGTGGACT GCTCTTCAAT TGATAAGCTG 
GACCTCTCAC CCTGGGCCTC AGTGAAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lpo ( XM_011249298.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011247600.1 Mus musculus lactoperoxidase (Lpo), transcript variant X1, mRNA.

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CloneID	OMu09629
Clone ID Related Accession (Same CDS sequence)	XM_011249298.3 , XM_011249298.2 , NM_080420.2
Accession Version	XM_011249298.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lactoperoxidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTGC TTCTGCATCT CCCAGCCCTC CTGGCTTCCC TGACCTTGCT CCAGACTGCG 
GCATCTGCCA GCGATGATCC AACTGCCGAG ACTGACATCA TCCACGACAC AGTGGAAGAG 
GTCAAGGTCT GGGTCAACAA GGCCTTCCTG GATTCCCGGG ACAGGCTGAA GATGGCCATG 
ACAACCAAGA TACATAGCAC CCGTCATCTG TCAGATTATC TCAAGCATGC CAAAGGCCGG 
ACACGCACAG CCATCCGGAG TGGGCAGGTG TGGGAAGAGT CCCTAAAGAA ACTGTCTCAG 
TTTCTCACCA ATGTCACAGG TCAAGGCCTG GACTTGACTT TGCTCTCTTG GGAGGCGGGC 
TGTGATCCCC CTGCTCCAAC AATGACTTGC AACATAAGTA GCCCTTACCG GACCATCACT 
GGATACTGTA ACAACAGGAA GAATCCAGCG CTGGGATCCG CCAACAGGGC GCTGGCTCGA 
TGGCTGCCCG CAGAATACGA GGACGGGCTC TCCCTCCCTT ACGGGTGGAC GCCGGGGAAG 
ATGCGGAACG GCTTCCCTCT CCCGCAGCCC CGTGAAGTCT CTAACCAGAT TGCAGCCTAT 
CTGAATGAGG AGGACGTTCT GGACCAGAAG AGATCCATGC TCTTCATGCA GTGGGGTCAA 
ATCGTGGACC ACGACATGGA CTTTGCCCCT GAAACTGAAA TGGGGAGTGA TACCTACACC 
AAAGCCCAGT GTGACGAACA CTGTATCCAG GGAGACAACT GCTTCCCCAT CATGTTCCCA 
CCTGGTGACC CTAAATTAAA GACTCAGGGG AAGTGCATGC CTTTCTTCAG AGCTGGATTT 
GTCTGCCCCA CACCACCCTA TAAATCCCTG GCCCGAGAGC AGATCAATGC TCTAACCTCT 
TTCCTGGATG CTAGCCTAGT GTATAGCCCT GAGCCAAGCC TGGCAAATCG ACTTAGAAAC 
CTCAGCAGCC CCTTGGGCCT CATGGCTGTC AATGAAGAAG TCTCAGACAA TGGGCGGCCC 
TTCCCGCCTT TCGTCAAAAT GAAACCCAGC CCCTGCGAGG TCATCAATGC CACAGCTGGT 
GTGCCCTGCT TCTTGGCAGG GGACTCCCGA GCCTCAGAGC AGATCTTGTT GGCCACATCC 
CACACACTCT TTATCCGGGA GCACAACCGG TTGGCCACAG AACTAAGCAG ACTCAACCCG 
CATTGGGACA GAGAGACGCT CTATCAGGAA GCAAGGAAAA TCATGGGAGC CTTCATACAG 
ATTACCACCT TTAGGGACTA CCTACCCATC CTACTGGGTG ATGAGATGCA GAAGTGGATT 
CCCCCATACC AAGGCTACAA TGAGTCTGTA GACCCCCGAA TTTCCAATGT CTTCACCTTT 
GCTTTACGCT TCGGCCACTT GGAGATCCCC TCTACTGTGT ACCGCCTAGA TGAGAATTAT 
CAGCCGTGGG GTTCAGAATC TGAGCTACCC CTGCACACTG TCTTTTTCAA CACCTGGAGG 
CTGGTCAAGG ATGGTGGAAT TGATCCTCTG GTTCGAGGTC TGTTGGCCAA GAATGCCAAG 
TTGATGCATC AGAACAAAAT GATGACAGGC GAGCTTCGAA ACAAGCTGTT CCAACCGAAT 
CACACCATCC ACGGCTTCGA CCTGGCTTCC ATCAACATAC AGCGGAGCAG AGACCACGGG 
CAGCCTGGGT ACAATTCCTG GAGAGCTTTC TGTGGCCTGT CCCAACCAAA AACACTAGAG 
GAGCTGAGTG CTGTCATGAA AAACGAGGTG CTGGCCAAGA AGCTGATGGA CCTCTATGGA 
ACCCCGAGCA ACATTGATAT CTGGCTAGGG GCCGTCGCTG AACCCCTGGT CCACAGGGGT 
CGGGTGGGAC CTCTCTTGAC CTGTCTCCTG GGCCAGCAGT TCCAGCGGAT CCGAGATGGA 
GACAGGTTCT GGTGGGAGAA CCCCGGGGTC TTCACAGAGA AGCAACGGGA GTCTCTACAG 
AAGATGTCCT TCTCCCGCCT GGTCTGTGAC AACACTGGCA TCGACAAGGT CCCACTGAAC 
CCGTTCCAGG CTAATGCCTA CCCCCATGGC TTTGTGGACT GCTCTTCAAT TGATAAGCTG 
GACCTCTCAC CCTGGGCCTC AGTGAAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011247600.1
CDS	223..2355
Translation	MKVLLHLPALLASLTLLQTAASASDDPTAETDIIHDTVEEVKVWVNKAFLDSRDRLKMAMTTKIHSTRHLSDYLKHAKGRTRTAIRSGQVWEESLKKLSQFLTNVTGQGLDLTLLSWEAGCDPPAPTMTCNISSPYRTITGYCNNRKNPALGSANRALARWLPAEYEDGLSLPYGWTPGKMRNGFPLPQPREVSNQIAAYLNEEDVLDQKRSMLFMQWGQIVDHDMDFAPETEMGSDTYTKAQCDEHCIQGDNCFPIMFPPGDPKLKTQGKCMPFFRAGFVCPTPPYKSLAREQINALTSFLDASLVYSPEPSLANRLRNLSSPLGLMAVNEEVSDNGRPFPPFVKMKPSPCEVINATAGVPCFLAGDSRASEQILLATSHTLFIREHNRLATELSRLNPHWDRETLYQEARKIMGAFIQITTFRDYLPILLGDEMQKWIPPYQGYNESVDPRISNVFTFALRFGHLEIPSTVYRLDENYQPWGSESELPLHTVFFNTWRLVKDGGIDPLVRGLLAKNAKLMHQNKMMTGELRNKLFQPNHTIHGFDLASINIQRSRDHGQPGYNSWRAFCGLSQPKTLEELSAVMKNEVLAKKLMDLYGTPSNIDIWLGAVAEPLVHRGRVGPLLTCLLGQQFQRIRDGDRFWWENPGVFTEKQRESLQKMSFSRLVCDNTGIDKVPLNPFQANAYPHGFVDCSSIDKLDLSPWASVKE

Target ORF information:

RefSeq Version	XM_011249298.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lactoperoxidase (Lpo), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249298.3

ORF Insert Sequence:

ATGAAAGTGC TTCTGCATCT CCCAGCCCTC CTGGCTTCCC TGACCTTGCT CCAGACTGCG 
GCATCTGCCA GCGATGATCC AACTGCCGAG ACTGACATCA TCCACGACAC AGTGGAAGAG 
GTCAAGGTCT GGGTCAACAA GGCCTTCCTG GATTCCCGGG ACAGGCTGAA GATGGCCATG 
ACAACCAAGA TACATAGCAC CCGTCATCTG TCAGATTATC TCAAGCATGC CAAAGGCCGG 
ACACGCACAG CCATCCGGAG TGGGCAGGTG TGGGAAGAGT CCCTAAAGAA ACTGTCTCAG 
TTTCTCACCA ATGTCACAGG TCAAGGCCTG GACTTGACTT TGCTCTCTTG GGAGGCGGGC 
TGTGATCCCC CTGCTCCAAC AATGACTTGC AACATAAGTA GCCCTTACCG GACCATCACT 
GGATACTGTA ACAACAGGAA GAATCCAGCG CTGGGATCCG CCAACAGGGC GCTGGCTCGA 
TGGCTGCCCG CAGAATACGA GGACGGGCTC TCCCTCCCTT ACGGGTGGAC GCCGGGGAAG 
ATGCGGAACG GCTTCCCTCT CCCGCAGCCC CGTGAAGTCT CTAACCAGAT TGCAGCCTAT 
CTGAATGAGG AGGACGTTCT GGACCAGAAG AGATCCATGC TCTTCATGCA GTGGGGTCAA 
ATCGTGGACC ACGACATGGA CTTTGCCCCT GAAACTGAAA TGGGGAGTGA TACCTACACC 
AAAGCCCAGT GTGACGAACA CTGTATCCAG GGAGACAACT GCTTCCCCAT CATGTTCCCA 
CCTGGTGACC CTAAATTAAA GACTCAGGGG AAGTGCATGC CTTTCTTCAG AGCTGGATTT 
GTCTGCCCCA CACCACCCTA TAAATCCCTG GCCCGAGAGC AGATCAATGC TCTAACCTCT 
TTCCTGGATG CTAGCCTAGT GTATAGCCCT GAGCCAAGCC TGGCAAATCG ACTTAGAAAC 
CTCAGCAGCC CCTTGGGCCT CATGGCTGTC AATGAAGAAG TCTCAGACAA TGGGCGGCCC 
TTCCCGCCTT TCGTCAAAAT GAAACCCAGC CCCTGCGAGG TCATCAATGC CACAGCTGGT 
GTGCCCTGCT TCTTGGCAGG GGACTCCCGA GCCTCAGAGC AGATCTTGTT GGCCACATCC 
CACACACTCT TTATCCGGGA GCACAACCGG TTGGCCACAG AACTAAGCAG ACTCAACCCG 
CATTGGGACA GAGAGACGCT CTATCAGGAA GCAAGGAAAA TCATGGGAGC CTTCATACAG 
ATTACCACCT TTAGGGACTA CCTACCCATC CTACTGGGTG ATGAGATGCA GAAGTGGATT 
CCCCCATACC AAGGCTACAA TGAGTCTGTA GACCCCCGAA TTTCCAATGT CTTCACCTTT 
GCTTTACGCT TCGGCCACTT GGAGATCCCC TCTACTGTGT ACCGCCTAGA TGAGAATTAT 
CAGCCGTGGG GTTCAGAATC TGAGCTACCC CTGCACACTG TCTTTTTCAA CACCTGGAGG 
CTGGTCAAGG ATGGTGGAAT TGATCCTCTG GTTCGAGGTC TGTTGGCCAA GAATGCCAAG 
TTGATGCATC AGAACAAAAT GATGACAGGC GAGCTTCGAA ACAAGCTGTT CCAACCGAAT 
CACACCATCC ACGGCTTCGA CCTGGCTTCC ATCAACATAC AGCGGAGCAG AGACCACGGG 
CAGCCTGGGT ACAATTCCTG GAGAGCTTTC TGTGGCCTGT CCCAACCAAA AACACTAGAG 
GAGCTGAGTG CTGTCATGAA AAACGAGGTG CTGGCCAAGA AGCTGATGGA CCTCTATGGA 
ACCCCGAGCA ACATTGATAT CTGGCTAGGG GCCGTCGCTG AACCCCTGGT CCACAGGGGT 
CGGGTGGGAC CTCTCTTGAC CTGTCTCCTG GGCCAGCAGT TCCAGCGGAT CCGAGATGGA 
GACAGGTTCT GGTGGGAGAA CCCCGGGGTC TTCACAGAGA AGCAACGGGA GTCTCTACAG 
AAGATGTCCT TCTCCCGCCT GGTCTGTGAC AACACTGGCA TCGACAAGGT CCCACTGAAC 
CCGTTCCAGG CTAATGCCTA CCCCCATGGC TTTGTGGACT GCTCTTCAAT TGATAAGCTG 
GACCTCTCAC CCTGGGCCTC AGTGAAGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Loss of miR-10a activates lpo and collaborates with activated Wnt signaling in inducing intestinal neoplasia in female mice.
PLoS genetics9(10)e1003913(2013 Oct)
Stadthagen G,Tehler D,H?yland-Kroghsbo NM,Wen J,Krogh A,Jensen KT,Santoni-Rugiu E,Engelholm LH,Lund AH

Expression of lactoperoxidase in differentiated mouse colon epithelial cells.
Free radical biology & medicine52(9)1569-76(2012 May)
Kim BW,Esworthy RS,Hahn MA,Pfeifer GP,Chu FF

The evolutionary conservation of the mammalian peroxidase genes.
Cytogenetic and genome research98(1)93-5(2002)
Sakamaki K,Ueda T,Nagata S

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Lpo cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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