Rhoh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rhoh
Entrez Gene ID	74734
Full Name	ras homolog family member H
Synonyms	5830400A04Rik,AU019774,Arhh
General protein information	Preferred Names ras homolog family member H Names rho-related GTP-binding protein RhoH ras homolog gene family, member H
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 C3.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081105.2	NP_001074574.1	rho-related GTP-binding protein RhoH precursor
XM_030254861.1	XP_030110721.1	rho-related GTP-binding protein RhoH isoform X1
NM_001081105.1	NP_001074574.1	rho-related GTP-binding protein RhoH precursor
XM_011240779.2	XP_011239081.1	rho-related GTP-binding protein RhoH isoform X1
XM_030254862.1	XP_030110722.1	rho-related GTP-binding protein RhoH isoform X1
NM_001363454.1	NP_001350383.1	rho-related GTP-binding protein RhoH precursor
XM_011240779.3	XP_011239081.1	rho-related GTP-binding protein RhoH isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rhoh	NP_001074574.1
Pan troglodytes (chimpanzee)	RHOH	XP_003950362.1
Danio rerio (zebrafish)	rhoh	XP_005171000.1
Homo sapiens (human)	RHOH	NP_001265292.1
	RHOH	XP_001094054.1
Bos taurus (cattle)	RHOH	NP_001071592.1
Gallus gallus (chicken)	RHOH	XP_004936171.1
Canis lupus familiaris (dog)	RHOH	XP_005618669.1
Rattus norvegicus (Norway rat)	Rhoh	NP_001013448.1
Xenopus tropicalis (tropical clawed frog)	rhoh	NP_001120435.1

Gene Ontology
Bibliography

Related articles in PubMed

??TCR recruits the Syk/PI3K axis to drive proinflammatory differentiation program.
Muro R, Nitta T, Nakano K, Okamura T, Takayanagi H, Suzuki H
The Journal of clinical investigation128(1)415-426(2018 Jan)

RhoH is a negative regulator of eosinophilopoiesis.
Stoeckle C, Geering B, Yousefi S, Ro?man S, Andina N, Benarafa C, Simon HU
Cell death and differentiation23(12)1961-1972(2016 Dec)

Overexpression of RhoH Permits to Bypass the Pre-TCR Checkpoint.
Tamehiro N, Oda H, Shirai M, Suzuki H
PloS one10(6)e0131047(2015)

A unique carboxyl-terminal insert domain in the hematopoietic-specific, GTPase-deficient Rho GTPase RhoH regulates post-translational processing.
Troeger A, Chae HD, Senturk M, Wood J, Williams DA
The Journal of biological chemistry288(51)36451-62(2013 Dec)

Human RHOH deficiency causes T cell defects and susceptibility to EV-HPV infections.
Crequer A, Troeger A, Patin E, Ma CS, Picard C, Pedergnana V, Fieschi C, Lim A, Abhyankar A, Gineau L, Mueller-Fleckenstein I, Schmidt M, Taieb A, Krueger J, Abel L, Tangye SG, Orth G, Williams DA, Casanova JL, Jouanguy E
The Journal of clinical investigation122(9)3239-47(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results highlight a novel negative regulatory role for RhoH in eosinophil differentiation, most likely in consequence of altered GATA-2 levels.
Title: RhoH is a negative regulator of eosinophilopoiesis.

An excess amount of RhoH is able to initiate pre-TCR signaling in the absence of pre-TCR complexes.
Title: Overexpression of RhoH Permits to Bypass the Pre-TCR Checkpoint.

The C-terminal insert domain regulates RhoH protein stability via chaperone-mediated autophagy.
Title: A unique carboxyl-terminal insert domain in the hematopoietic-specific, GTPase-deficient Rho GTPase RhoH regulates post-translational processing.

RhoH plays a critical role in prosurvival chronic lymphocytic leukemia cell-cell and cell-microenvironment interactions with this agent.
Title: RhoH is critical for cell-microenvironment interactions in chronic lymphocytic leukemia in mice and humans.

These findings stress the importance of Rhoh for the function of peripheral T cells.
Title: Rhoh deficiency reduces peripheral T-cell function and attenuates allogenic transplant rejection.

The following Rhoh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rhoh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13512	NM_001081105.2 Latest version!	Mus musculus ras homolog family member H (Rhoh), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu13512	NM_001363454.1 Latest version!	Mus musculus ras homolog family member H (Rhoh), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu13512	XM_011240779.3 Latest version!	Mus musculus ras homolog family member H (Rhoh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu13512	XM_030254861.1 Latest version!	Mus musculus ras homolog family member H (Rhoh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu13512	XM_030254862.1 Latest version!	Mus musculus ras homolog family member H (Rhoh), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu13512	NM_001081105.1	Mus musculus ras homolog family member H (Rhoh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu13512	XM_011240779.2	Mus musculus ras homolog family member H (Rhoh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rhoh ( NM_001081105.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074574.1 Mus musculus ras homolog family member H (Rhoh), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13512
Clone ID Related Accession (Same CDS sequence)	NM_001081105.2 , XM_030254861.1 , NM_001081105.1 , XM_011240779.2 , XM_030254862.1 , NM_001363454.1 , XM_011240779.3
Accession Version	NM_001081105.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoH precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC156029.2. On May 18, 2018 this sequence version replaced NM_001081105.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK169618.1, AK017885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074574.1
CDS	634..1209
Translation	MLSSIKCVLVGDSAVGKTSLLVRFTSETFPEAYKPTVYENTGVDVFMDGIQISLGLWDTAGNDAFRSIRPLSYQQADVVLMCYSVANHNSFLNLKNKWISEIRSNLPCTPVLVVATQTDQREVGPHRASCINAIEGKRLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRKLFSINECKIF

Target ORF information:

RefSeq Version	NM_001081105.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member H (Rhoh), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081105.2

ORF Insert Sequence:

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhoh ( NM_001081105.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074574.1 Mus musculus ras homolog family member H (Rhoh), mRNA.

Price & Availability↑

CloneID	OMu13512
Clone ID Related Accession (Same CDS sequence)	NM_001081105.2 , XM_030254861.1 , NM_001081105.1 , XM_011240779.2 , XM_030254862.1 , NM_001363454.1 , XM_011240779.3
Accession Version	NM_001081105.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoH precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC156029.2. On Feb 6, 2007 this sequence version replaced XM_132051.7. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK169618.1, AK017885.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074574.1
CDS	649..1224
Translation	MLSSIKCVLVGDSAVGKTSLLVRFTSETFPEAYKPTVYENTGVDVFMDGIQISLGLWDTAGNDAFRSIRPLSYQQADVVLMCYSVANHNSFLNLKNKWISEIRSNLPCTPVLVVATQTDQREVGPHRASCINAIEGKRLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRKLFSINECKIF

Target ORF information:

RefSeq Version	NM_001081105.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member H (Rhoh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081105.1

ORF Insert Sequence:

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhoh ( NM_001363454.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001350383.1 Mus musculus ras homolog family member H (Rhoh), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu13512
Clone ID Related Accession (Same CDS sequence)	NM_001081105.2 , XM_030254861.1 , NM_001081105.1 , XM_011240779.2 , XM_030254862.1 , NM_001363454.1 , XM_011240779.3
Accession Version	NM_001363454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoH precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK138090.1, AC156029.2, AK137909.1 and AI448508.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK138090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350383.1
CDS	775..1350
Translation	MLSSIKCVLVGDSAVGKTSLLVRFTSETFPEAYKPTVYENTGVDVFMDGIQISLGLWDTAGNDAFRSIRPLSYQQADVVLMCYSVANHNSFLNLKNKWISEIRSNLPCTPVLVVATQTDQREVGPHRASCINAIEGKRLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRKLFSINECKIF

Target ORF information:

RefSeq Version	NM_001363454.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member H (Rhoh), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363454.1

ORF Insert Sequence:

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhoh ( XM_011240779.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239081.1 Mus musculus ras homolog family member H (Rhoh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13512
Clone ID Related Accession (Same CDS sequence)	NM_001081105.2 , XM_030254861.1 , NM_001081105.1 , XM_011240779.2 , XM_030254862.1 , NM_001363454.1 , XM_011240779.3
Accession Version	XM_011240779.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoH isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011240779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239081.1
CDS	1323..1898
Translation	MLSSIKCVLVGDSAVGKTSLLVRFTSETFPEAYKPTVYENTGVDVFMDGIQISLGLWDTAGNDAFRSIRPLSYQQADVVLMCYSVANHNSFLNLKNKWISEIRSNLPCTPVLVVATQTDQREVGPHRASCINAIEGKRLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRKLFSINECKIF

Target ORF information:

RefSeq Version	XM_011240779.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member H (Rhoh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240779.2

ORF Insert Sequence:

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhoh ( XM_030254861.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110721.1 Mus musculus ras homolog family member H (Rhoh), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu13512
Clone ID Related Accession (Same CDS sequence)	NM_001081105.2 , XM_030254861.1 , NM_001081105.1 , XM_011240779.2 , XM_030254862.1 , NM_001363454.1 , XM_011240779.3
Accession Version	XM_030254861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoH isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110721.1
CDS	1679..2254
Translation	MLSSIKCVLVGDSAVGKTSLLVRFTSETFPEAYKPTVYENTGVDVFMDGIQISLGLWDTAGNDAFRSIRPLSYQQADVVLMCYSVANHNSFLNLKNKWISEIRSNLPCTPVLVVATQTDQREVGPHRASCINAIEGKRLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRKLFSINECKIF

Target ORF information:

RefSeq Version	XM_030254861.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member H (Rhoh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254861.1

ORF Insert Sequence:

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhoh ( XM_030254862.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110722.1 Mus musculus ras homolog family member H (Rhoh), transcript variant X3, mRNA.

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CloneID	OMu13512
Clone ID Related Accession (Same CDS sequence)	NM_001081105.2 , XM_030254861.1 , NM_001081105.1 , XM_011240779.2 , XM_030254862.1 , NM_001363454.1 , XM_011240779.3
Accession Version	XM_030254862.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoH isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110722.1
CDS	1082..1657
Translation	MLSSIKCVLVGDSAVGKTSLLVRFTSETFPEAYKPTVYENTGVDVFMDGIQISLGLWDTAGNDAFRSIRPLSYQQADVVLMCYSVANHNSFLNLKNKWISEIRSNLPCTPVLVVATQTDQREVGPHRASCINAIEGKRLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRKLFSINECKIF

Target ORF information:

RefSeq Version	XM_030254862.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member H (Rhoh), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254862.1

ORF Insert Sequence:

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rhoh ( XM_011240779.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011239081.1 Mus musculus ras homolog family member H (Rhoh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu13512
Clone ID Related Accession (Same CDS sequence)	NM_001081105.2 , XM_030254861.1 , NM_001081105.1 , XM_011240779.2 , XM_030254862.1 , NM_001363454.1 , XM_011240779.3
Accession Version	XM_011240779.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho-related GTP-binding protein RhoH isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240779.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011239081.1
CDS	1520..2095
Translation	MLSSIKCVLVGDSAVGKTSLLVRFTSETFPEAYKPTVYENTGVDVFMDGIQISLGLWDTAGNDAFRSIRPLSYQQADVVLMCYSVANHNSFLNLKNKWISEIRSNLPCTPVLVVATQTDQREVGPHRASCINAIEGKRLAQDVRAKGYLECSALSNRGVQQVFECAVRTAVNQARRRNRRKLFSINECKIF

Target ORF information:

RefSeq Version	XM_011240779.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ras homolog family member H (Rhoh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240779.3

ORF Insert Sequence:

ATGCTGAGCT CAATCAAGTG CGTGCTGGTA GGGGACAGTG CTGTGGGGAA AACCTCACTG 
TTGGTGCGCT TCACCTCTGA GACCTTCCCG GAGGCCTACA AACCCACGGT GTACGAGAAT 
ACGGGTGTAG ACGTCTTCAT GGATGGCATC CAGATCAGCC TGGGTCTCTG GGACACTGCC 
GGCAACGACG CCTTCAGAAG TATCCGGCCC CTGTCCTACC AGCAGGCAGA CGTGGTACTG 
ATGTGCTACT CTGTGGCCAA CCATAACTCG TTCCTGAACT TGAAGAACAA ATGGATTAGT 
GAGATCAGGA GCAACCTACC CTGTACCCCG GTGCTGGTTG TGGCCACACA GACGGACCAG 
AGAGAGGTGG GACCTCACAG GGCTTCCTGC ATCAATGCCA TAGAAGGGAA GAGACTTGCC 
CAGGATGTGA GAGCCAAGGG CTACCTGGAG TGCTCAGCCC TCAGCAACCG GGGAGTACAG 
CAGGTATTTG AATGTGCTGT CCGAACAGCT GTCAACCAGG CCAGGAGGCG AAACAGAAGG 
AAGCTGTTCT CCATCAATGA ATGCAAGATC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

??TCR recruits the Syk/PI3K axis to drive proinflammatory differentiation program.
The Journal of clinical investigation128(1)415-426(2018 Jan)
Muro R,Nitta T,Nakano K,Okamura T,Takayanagi H,Suzuki H

RhoH is a negative regulator of eosinophilopoiesis.
Cell death and differentiation23(12)1961-1972(2016 Dec)
Stoeckle C,Geering B,Yousefi S,Ro?man S,Andina N,Benarafa C,Simon HU

Overexpression of RhoH Permits to Bypass the Pre-TCR Checkpoint.
PloS one10(6)e0131047(2015)
Tamehiro N,Oda H,Shirai M,Suzuki H

A unique carboxyl-terminal insert domain in the hematopoietic-specific, GTPase-deficient Rho GTPase RhoH regulates post-translational processing.
The Journal of biological chemistry288(51)36451-62(2013 Dec)
Troeger A,Chae HD,Senturk M,Wood J,Williams DA

Human RHOH deficiency causes T cell defects and susceptibility to EV-HPV infections.
The Journal of clinical investigation122(9)3239-47(2012 Sep)
Crequer A,Troeger A,Patin E,Ma CS,Picard C,Pedergnana V,Fieschi C,Lim A,Abhyankar A,Gineau L,Mueller-Fleckenstein I,Schmidt M,Taieb A,Krueger J,Abel L,Tangye SG,Orth G,Williams DA,Casanova JL,Jouanguy E

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Rhoh cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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