Mrpl47 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mrpl47
Entrez Gene ID	74600
Full Name	mitochondrial ribosomal protein L47
Synonyms	4833424P18Rik,CGI-204,Gm9859,L47mt,MRP-L47,MTF/L47,NCM1
General protein information	Preferred Names mitochondrial ribosomal protein L47 Names 39S ribosomal protein L47, mitochondrial metanephros-derived tubulogenic factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 A3
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BRG1/brm-associated factor 53A (also known as BAF complex 53 kDa subunit protein A in humans) in a tail-to-tail orientation. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_029017.2	NP_083293.1	39S ribosomal protein L47, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	MRPL47	NP_065142.2
Pan troglodytes (chimpanzee)	MRPL47	XP_003950281.1
Rattus norvegicus (Norway rat)	Mrpl47	NP_001032260.1
Gallus gallus (chicken)	MRPL47	XP_422785.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002218	XP_307966.3
Canis lupus familiaris (dog)	MRPL47	XP_005639793.1
Drosophila melanogaster (fruit fly)	mRpL47	NP_788610.1
Xenopus tropicalis (tropical clawed frog)	mrpl47	NP_001107535.2
	MRPL47	XP_001108605.1
Bos taurus (cattle)	MRPL47	NP_001070369.1
Mus musculus (house mouse)	Mrpl47	NP_083293.1
Danio rerio (zebrafish)	LOC557789	XP_686002.1

Gene Ontology
Bibliography

Related articles in PubMed

Cloning and characterization of a novel gene promoting ureteric bud branching in the metanephros.
Araki T, Hayashi M, Saruta T
Kidney international64(6)1968-77(2003 Dec)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Novel molecule in developing kidney that is capable of modulating ureteric bud branching and kidney differentiation. [Metanephros-derived tubulogenic factor]
Title: Cloning and characterization of a novel gene promoting ureteric bud branching in the metanephros.

The following Mrpl47 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mrpl47 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13335	NM_029017.2 Latest version!	Mus musculus mitochondrial ribosomal protein L47 (Mrpl47), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mrpl47 ( NM_029017.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083293.1 Mus musculus mitochondrial ribosomal protein L47 (Mrpl47), mRNA.

Price & Availability↑

CloneID	OMu13335
Clone ID Related Accession (Same CDS sequence)	NM_029017.2
Accession Version	NM_029017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	39S ribosomal protein L47, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY013327.1, CX122942.1 and AA174956.1. On Mar 5, 2010 this sequence version replaced NM_029017.1. ##Evidence-Data-START## Transcript exon combination :: BC087953.1, BQ884589.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGA CCAGTCTAGT GGGTATTTGT AGAAGAGCCT CAGCGTTCCT GAAGGCAGCT 
TGTTCCCTAG TAAATCCCAA GGACGCTGCT CACTCGGGTT GCAGGTCTTC TCTTAGTTTG 
TTACATAAGA ACACACCACA TGTCACATCT TTTCTCCAGT GTAAATTACT TCATACCACG 
TTGTCAAGGA AAGGACTGGA AGAATTTTTT GATGACCCAA AGAATTGGGG GGAAGAAAAA 
GTCAAATCTG GAGCTTCATG GACCTGCCAG CAGCTGAGGA ACAAAAGTAA CGAAGACTTA 
CATAAGCTTT GGTATGTCCT TCTGAAGGAA AGAAACATGC TTCTAACTCT GGAGCAGGAG 
GCCAAGCGAC AGAGGTTGCC AATGCCAAGT CCGGAGCGCT TAGAAAAGGT CGTTGATTCC 
ATGGATAACG TAGATAAAGT TGTCCAGGAG AGGGAAGATG CTCTAAGGCT TCTTCAGACC 
GGTCAAGAAA AGCCCAGACC CGGTGCTTGG AGAAGGGACA TCTTTGGACG AATTGTCTGG 
CACAAATTCA AGCAGTGGCC TATACCTTGG TACCTAAATA AAAGATACAA CAGGAGGCGG 
TTCTTCGCAA TGCCTTATGT GGATCGCTTT ATCAGACTAA GAATTGAGAA ACACGCCCGC 
ATTGAAGCAA GAAAGAGAAG TTTACAGAAA AAGAAAGAAA AAATTCTCCA TGCAAAGTTC 
CCACATCTCT CTCAAGAACG GAAATCAAGT AGTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083293.1
CDS	17..775
Translation	MAATSLVGICRRASAFLKAACSLVNPKDAAHSGCRSSLSLLHKNTPHVTSFLQCKLLHTTLSRKGLEEFFDDPKNWGEEKVKSGASWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLEKVVDSMDNVDKVVQEREDALRLLQTGQEKPRPGAWRRDIFGRIVWHKFKQWPIPWYLNKRYNRRRFFAMPYVDRFIRLRIEKHARIEARKRSLQKKKEKILHAKFPHLSQERKSSSV

Target ORF information:

RefSeq Version	NM_029017.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial ribosomal protein L47 (Mrpl47), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029017.2

ORF Insert Sequence:

ATGGCTGCGA CCAGTCTAGT GGGTATTTGT AGAAGAGCCT CAGCGTTCCT GAAGGCAGCT 
TGTTCCCTAG TAAATCCCAA GGACGCTGCT CACTCGGGTT GCAGGTCTTC TCTTAGTTTG 
TTACATAAGA ACACACCACA TGTCACATCT TTTCTCCAGT GTAAATTACT TCATACCACG 
TTGTCAAGGA AAGGACTGGA AGAATTTTTT GATGACCCAA AGAATTGGGG GGAAGAAAAA 
GTCAAATCTG GAGCTTCATG GACCTGCCAG CAGCTGAGGA ACAAAAGTAA CGAAGACTTA 
CATAAGCTTT GGTATGTCCT TCTGAAGGAA AGAAACATGC TTCTAACTCT GGAGCAGGAG 
GCCAAGCGAC AGAGGTTGCC AATGCCAAGT CCGGAGCGCT TAGAAAAGGT CGTTGATTCC 
ATGGATAACG TAGATAAAGT TGTCCAGGAG AGGGAAGATG CTCTAAGGCT TCTTCAGACC 
GGTCAAGAAA AGCCCAGACC CGGTGCTTGG AGAAGGGACA TCTTTGGACG AATTGTCTGG 
CACAAATTCA AGCAGTGGCC TATACCTTGG TACCTAAATA AAAGATACAA CAGGAGGCGG 
TTCTTCGCAA TGCCTTATGT GGATCGCTTT ATCAGACTAA GAATTGAGAA ACACGCCCGC 
ATTGAAGCAA GAAAGAGAAG TTTACAGAAA AAGAAAGAAA AAATTCTCCA TGCAAAGTTC 
CCACATCTCT CTCAAGAACG GAAATCAAGT AGTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cloning and characterization of a novel gene promoting ureteric bud branching in the metanephros.
Kidney international64(6)1968-77(2003 Dec)
Araki T,Hayashi M,Saruta T

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Mrpl47 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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