Ttf2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ttf2
Entrez Gene ID	74044
Full Name	transcription termination factor, RNA polymerase II
Synonyms	4632434F22Rik,8030447N19,AV218430
General protein information	Preferred Names transcription termination factor, RNA polymerase II Names transcription termination factor 2 RNA polymerase II termination factor transcription release factor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006502149.1	XP_006502212.1	transcription termination factor 2 isoform X1
NM_001013026.2	NP_001013044.2	transcription termination factor 2
XM_006502149.2	XP_006502212.1	transcription termination factor 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TTF2	XP_005630717.1
Bos taurus (cattle)	TTF2	NP_001074204.1
Caenorhabditis elegans (roundworm)	F54E12.2	NP_502137.1
Homo sapiens (human)	TTF2	NP_003585.3
	TTF2	XP_001112974.1
Rattus norvegicus (Norway rat)	Ttf2	NP_001099924.2
Pan troglodytes (chimpanzee)	TTF2	XP_513683.2
Danio rerio (zebrafish)	si:ch1073-303l5.1	XP_005168676.1
Caenorhabditis elegans (roundworm)	T23H2.3	NP_001032980.2
Mus musculus (house mouse)	Ttf2	NP_001013044.2
Gallus gallus (chicken)	TTF2	XP_004938292.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of mouse TTF-2 gene causes cleft palate.
Meng T, Shi JY, Wu M, Wang Y, Li L, Liu Y, Zheng Q, Huang L, Shi B
Journal of cellular and molecular medicine16(10)2362-8(2012 Oct)

Embryonic epithelial Pten deletion through Nkx2.1-cre leads to thyroid tumorigenesis in a strain-dependent manner.
Tiozzo C, Danopoulos S, Lavarreda-Pearce M, Baptista S, Varimezova R, Al Alam D, Warburton D, Virender R, De Langhe S, Di Cristofano A, Bellusci S, Minoo P
Endocrine-related cancer19(2)111-122(2012 Apr)

Cytochrome P450 2E1-dependent oxidant stress and upregulation of anti-oxidant defense in liver cells.
Cederbaum AI
Journal of gastroenterology and hepatology21 Suppl 3S22-5(2006 Oct)

Identification of a structurally distinct CD101 molecule encoded in the 950-kb Idd10 region of NOD mice.
Penha-Gon?alves C, Moule C, Smink LJ, Howson J, Gregory S, Rogers J, Lyons PA, Suttie JJ, Lord CJ, Peterson LB, Todd JA, Wicker LS
Diabetes52(6)1551-6(2003 Jun)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of TTF-2 during the palatogenesis may contribute to cleft palate.
Title: Overexpression of mouse TTF-2 gene causes cleft palate.

The following Ttf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ttf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11412	NM_001013026.2 Latest version!	Mus musculus transcription termination factor, RNA polymerase II (Ttf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$671.30 $959.00
OMu32338	XM_006502149.2 Latest version!	Mus musculus transcription termination factor, RNA polymerase II (Ttf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu32338	XM_006502149.1	Mus musculus transcription termination factor, RNA polymerase II (Ttf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ttf2 ( NM_001013026.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001013044.2 Mus musculus transcription termination factor, RNA polymerase II (Ttf2), mRNA.

Price & Availability↑

CloneID	OMu11412
Clone ID Related Accession (Same CDS sequence)	NM_001013026.2
Accession Version	NM_001013026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	transcription termination factor 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK087352.1, BC096625.1 and BC087733.1. On Jul 25, 2009 this sequence version replaced NM_001013026.1. ##Evidence-Data-START## Transcript exon combination :: BC087733.1, AK014607.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCTGG TTAAGTGTCC GGAGCACGGG GACGCCTGCT TCTTAAAGAC CGGCGTCCGC 
GATGGCCCGA ATAAGGGAAA GAGCTTCTAC GTGTGCCGGA CGAACACATG CGGCTTCGTG 
CAGGCCACCG ATATTCCTGT TTCTCACTGT TTATTGCATG AGGAGTTTGT GGTCGAGCTT 
CAGGGTTTGT TTCTACCACA GGACAAGAAA GAATGGAGGC TGTTCTTCCG ATGTGCTAGA 
ACCAAGGCGG AGGGGAAGCA GTGGTGTGGA AACGTCCCAT GGCGGCAGGA TCCTAACCCT 
AAAGAACTCT CTGTAACCAG TAAGCCTCAG CAGCCATCTG AGTCACAGCT TCACTCTCCC 
AGCCAGCCGA GAAACCCATT TAGGGTGCTT AACAAGAATC AAAAAACACT CGAACGAAAA 
CAGTTTGTCG AAGAAGGTGA GAGAAAGACA GCTGATAAGA AGCTGAGAGA AAACAATGAG 
CAGCTCTTGG ATCAAAGGAA AGAACAGAAG CCCAAATCTA ACAGCAGGAT GGAGAAGGAC 
CCCTCATCTG ACCTGGTGGC AACTAGACAG TCTGGAGGTG ACAGGGAAGA ACAAGAGAAA 
TCAAAGTTCC AGCCCAAAAC TAAGAAAGCT GAAGGAATGG CTTCCAAGCA AGGCCATGGA 
GAGGTGCTTC AAGGAATACC CAAGGGTCCT CACATGTCAG AGAGTGAGAG TCGAGGGGTC 
CCGAACAAGC CAGAGACTCT GAGAGAAAAG GAAACCCAGC TTTTGGTGCC AAGTGTTCCT 
GGTCAGAACC CAGAAAGCAA GGTCCAGAAA GAGGGACACG TCAGCAGGGA GCCCCTCAAG 
AACGGGGAGG CCCCTAGTGC ACAGGTCACC CAGAGGGGGC TCGCTCAGGG GCCTCTCCAG 
GGACCATCCA AGACTTGGAG GCCTGTTCCT GAAGCACCAG CAGCACCTGA GCTTTGCTCG 
GGAATGGCCC ACCATGCCAC CTCCAGCAGT GAGGACAGCG AAGATGATGG TGTTTCCAGT 
AGGCCGGGGT CCCCACTTCT CTTTGACTCG ACTGTGGACT CACAGAAGAA GGGGAGCCTT 
CAGCATTCTG ACCAGAGTGT GCAGAGACAG ATGCCTGCTG CTTCAGGTGT TTCCAAGAAG 
GGAGATTCCT CTGACCCAGC AGCCCAGCGT GCAAACCTAA CAACACAGCT GAAACAAAAG 
AAGGGTACCC TGGCTGCAGT GAACATCCAG GCTCTCCCTG ATAAGGGTGA GAAGTTGCTT 
AAGCAAATCC AGGCTTTGGA GGACGCACTC AGTGCTCTGG CTCTCTCCCC AGAACAAGGA 
ACTAAAGAGA AGTGCAGCGC TCAGGAACCA GAGCAGAGCA ACATCACCAA AGCTGCTGCT 
GCCCCTCTCC ACTTGGTACC TCCCCAGCCC CTTCCCAGGC CCCTCATCCA GCCTGCAAGC 
TCTCTAGGAC TAAAGGCAGG CCGCCAGGAA ACTCCTGAGG GAGCCAGCCA GTGCTCAGGA 
GGCCATATGA ACCAACATCA TCTTTATAAT GTGTGGAAAA TCACGAGTGA AGCCATAGAT 
GAGCTGCATC GGTCACTGAA GTCTTGTCCG GGTGAAACAG CAGTAGCAGA AGACCCAGCT 
GGCTTGAAGG TCCCCTTGCT ACTGCACCAG AAGCAGGCAT TAGCCTGGTT ACTGTGGAGG 
GAAAGTCAGA AGCCACAAGG AGGGATCCTG GCGGATGATA TGGGCTTAGG AAAGACTCTG 
ACAATGATTG CACTCATCCT AACCAAGAAG AATCAGCAGA AAAGCAAGGA AAAGGAGAGA 
AGCGAGCCTG TGACGTGGCT TTCCAAGAAT GACTCCTCTG TCTTTACTTC CAGTGGAACA 
CTGATCGTCT GCCCCGCTTC CCTGATCCAT CACTGGAAGA ACGAAGTAGA GAAACGTGTA 
ACCAGCAACA GACTCAGAAT CTATCTCTAC CATGGGCCAA ACCGGAGTCG GCACGCAAAA 
GTACTCTCTA CGTATGACAT CGTGATTACT ACTTACAGCC TTCTGGCCAA GGAGATTCCT 
ACAACGAAGC AAGAGGGAGA GGTCCCAGGA GCAAACCTCA GTGTGGAGGG TACCTCAGCA 
CCTTTGCTTC AGGTAGTCTG GGCTCGGATC ATTTTGGATG AAGCTCATAA CGTGAAAAAC 
CCTCGAGTAC AGACATCCAT TGCTGTGTGT AAGCTCCAAG CCCAGGCCCG GTGGGCTGTC 
ACTGGAACCC CCATTCAGAA CAACCTGTTG GATATGTATT CACTGATGAA GTTTCTCCGT 
TGCTCCCCAT TTGATGAGTT CAGTCTTTGG AAGAGCCAGG TTGACAATGG TTCAATGAAA 
GGAGGGGAAC GGTTAAGCAT TTTAACCAAG AGCCTTTTGC TGAGGAGAAC AAAAGACCAG 
CTGGATTCCA CTGGTAAACC TCTGGTAGCA CTGCCTGCAC GTAGATGCCA GCTGCACCGG 
TTAAAGCTTT CTGAGGACGA GCGGGCCGTT TATGACATCT TTCTTGCAAG GTCAAGGTCA 
GCTCTCCAGT CCTATCTAAA GAGACAAGAA GGTAGAGGCA GCCACCATGG AAGAAGCCCT 
GATAATCCAT TCAGTAGAGT GGCTCAGGAG TTTGGATCCA GTGTGTCCCA GGGCTGCCCT 
GCGGCAGACT CCCAAAGACC CAGCACCGTC CATGTCCTGT CCCAGCTTCT GCGGCTTCGC 
CAGTGCTGCT GCCATCTTTC TCTGCTGAAG TCGGCTCTGG ACCCCACAGA ACTGGAGAGC 
GAAGGCCTTG TCCTGTCCCT GGAAGAACAG CTCAGTGCTC TGACCTTGTC CAAGGTCGAT 
GTCTCAGAGC CATCACCCAC GGTTAGCCTT AATGGCACAT GTTTCAAGGC GGAGCTTTTT 
GATGACACAC GAAGGAGCAC CAAGGTGTCT TCTCTGCTGG CAGAACTGGA GGCGATCCAA 
AAAGGCCCAG GGTCCCAAAA GAGTGTCATT GTCTCTCAGT GGACCAGCAT GCTGCAGGTA 
GTAGCACTGC ACCTCAAGAA GAACCGACTG ACTTACGCCA CCATCGATGG CTCTGTCAAC 
CCCAAACAGA GAATGGACCT GGTGGAGGCA TTTAACCACT CCCAGGGACC TCAGGTCATG 
CTGATCTCCC TCTTGGCTGG CGGTGTTGGA CTTAACCTGA CTGGAGGAAA CCACCTTTTC 
CTTCTGGACA TGCACTGGAA TCCCTCACTT GAAGACCAAG CTTGTGACCG GATTTACCGA 
GTAGGACAGA AGAAGGATGT TGTCATACAC AGGTTTGTCT GTGAGGGGAC AGTGGAAGAG 
AAGATCTTAC AGCTCCAAGA AAAAAAGAAA GATTTGGCAA AACAGGTCCT TTCTGGATCC 
GAAGGACCTG TCACCAAGCT CACACTGGCT GACCTCAAGA TCCTCTTTGG CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013044.2
CDS	17..3433
Translation	MDLVKCPEHGDACFLKTGVRDGPNKGKSFYVCRTNTCGFVQATDIPVSHCLLHEEFVVELQGLFLPQDKKEWRLFFRCARTKAEGKQWCGNVPWRQDPNPKELSVTSKPQQPSESQLHSPSQPRNPFRVLNKNQKTLERKQFVEEGERKTADKKLRENNEQLLDQRKEQKPKSNSRMEKDPSSDLVATRQSGGDREEQEKSKFQPKTKKAEGMASKQGHGEVLQGIPKGPHMSESESRGVPNKPETLREKETQLLVPSVPGQNPESKVQKEGHVSREPLKNGEAPSAQVTQRGLAQGPLQGPSKTWRPVPEAPAAPELCSGMAHHATSSSEDSEDDGVSSRPGSPLLFDSTVDSQKKGSLQHSDQSVQRQMPAASGVSKKGDSSDPAAQRANLTTQLKQKKGTLAAVNIQALPDKGEKLLKQIQALEDALSALALSPEQGTKEKCSAQEPEQSNITKAAAAPLHLVPPQPLPRPLIQPASSLGLKAGRQETPEGASQCSGGHMNQHHLYNVWKITSEAIDELHRSLKSCPGETAVAEDPAGLKVPLLLHQKQALAWLLWRESQKPQGGILADDMGLGKTLTMIALILTKKNQQKSKEKERSEPVTWLSKNDSSVFTSSGTLIVCPASLIHHWKNEVEKRVTSNRLRIYLYHGPNRSRHAKVLSTYDIVITTYSLLAKEIPTTKQEGEVPGANLSVEGTSAPLLQVVWARIILDEAHNVKNPRVQTSIAVCKLQAQARWAVTGTPIQNNLLDMYSLMKFLRCSPFDEFSLWKSQVDNGSMKGGERLSILTKSLLLRRTKDQLDSTGKPLVALPARRCQLHRLKLSEDERAVYDIFLARSRSALQSYLKRQEGRGSHHGRSPDNPFSRVAQEFGSSVSQGCPAADSQRPSTVHVLSQLLRLRQCCCHLSLLKSALDPTELESEGLVLSLEEQLSALTLSKVDVSEPSPTVSLNGTCFKAELFDDTRRSTKVSSLLAELEAIQKGPGSQKSVIVSQWTSMLQVVALHLKKNRLTYATIDGSVNPKQRMDLVEAFNHSQGPQVMLISLLAGGVGLNLTGGNHLFLLDMHWNPSLEDQACDRIYRVGQKKDVVIHRFVCEGTVEEKILQLQEKKKDLAKQVLSGSEGPVTKLTLADLKILFGI

Target ORF information:

RefSeq Version	NM_001013026.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transcription termination factor, RNA polymerase II (Ttf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013026.2

ORF Insert Sequence:

ATGGACCTGG TTAAGTGTCC GGAGCACGGG GACGCCTGCT TCTTAAAGAC CGGCGTCCGC 
GATGGCCCGA ATAAGGGAAA GAGCTTCTAC GTGTGCCGGA CGAACACATG CGGCTTCGTG 
CAGGCCACCG ATATTCCTGT TTCTCACTGT TTATTGCATG AGGAGTTTGT GGTCGAGCTT 
CAGGGTTTGT TTCTACCACA GGACAAGAAA GAATGGAGGC TGTTCTTCCG ATGTGCTAGA 
ACCAAGGCGG AGGGGAAGCA GTGGTGTGGA AACGTCCCAT GGCGGCAGGA TCCTAACCCT 
AAAGAACTCT CTGTAACCAG TAAGCCTCAG CAGCCATCTG AGTCACAGCT TCACTCTCCC 
AGCCAGCCGA GAAACCCATT TAGGGTGCTT AACAAGAATC AAAAAACACT CGAACGAAAA 
CAGTTTGTCG AAGAAGGTGA GAGAAAGACA GCTGATAAGA AGCTGAGAGA AAACAATGAG 
CAGCTCTTGG ATCAAAGGAA AGAACAGAAG CCCAAATCTA ACAGCAGGAT GGAGAAGGAC 
CCCTCATCTG ACCTGGTGGC AACTAGACAG TCTGGAGGTG ACAGGGAAGA ACAAGAGAAA 
TCAAAGTTCC AGCCCAAAAC TAAGAAAGCT GAAGGAATGG CTTCCAAGCA AGGCCATGGA 
GAGGTGCTTC AAGGAATACC CAAGGGTCCT CACATGTCAG AGAGTGAGAG TCGAGGGGTC 
CCGAACAAGC CAGAGACTCT GAGAGAAAAG GAAACCCAGC TTTTGGTGCC AAGTGTTCCT 
GGTCAGAACC CAGAAAGCAA GGTCCAGAAA GAGGGACACG TCAGCAGGGA GCCCCTCAAG 
AACGGGGAGG CCCCTAGTGC ACAGGTCACC CAGAGGGGGC TCGCTCAGGG GCCTCTCCAG 
GGACCATCCA AGACTTGGAG GCCTGTTCCT GAAGCACCAG CAGCACCTGA GCTTTGCTCG 
GGAATGGCCC ACCATGCCAC CTCCAGCAGT GAGGACAGCG AAGATGATGG TGTTTCCAGT 
AGGCCGGGGT CCCCACTTCT CTTTGACTCG ACTGTGGACT CACAGAAGAA GGGGAGCCTT 
CAGCATTCTG ACCAGAGTGT GCAGAGACAG ATGCCTGCTG CTTCAGGTGT TTCCAAGAAG 
GGAGATTCCT CTGACCCAGC AGCCCAGCGT GCAAACCTAA CAACACAGCT GAAACAAAAG 
AAGGGTACCC TGGCTGCAGT GAACATCCAG GCTCTCCCTG ATAAGGGTGA GAAGTTGCTT 
AAGCAAATCC AGGCTTTGGA GGACGCACTC AGTGCTCTGG CTCTCTCCCC AGAACAAGGA 
ACTAAAGAGA AGTGCAGCGC TCAGGAACCA GAGCAGAGCA ACATCACCAA AGCTGCTGCT 
GCCCCTCTCC ACTTGGTACC TCCCCAGCCC CTTCCCAGGC CCCTCATCCA GCCTGCAAGC 
TCTCTAGGAC TAAAGGCAGG CCGCCAGGAA ACTCCTGAGG GAGCCAGCCA GTGCTCAGGA 
GGCCATATGA ACCAACATCA TCTTTATAAT GTGTGGAAAA TCACGAGTGA AGCCATAGAT 
GAGCTGCATC GGTCACTGAA GTCTTGTCCG GGTGAAACAG CAGTAGCAGA AGACCCAGCT 
GGCTTGAAGG TCCCCTTGCT ACTGCACCAG AAGCAGGCAT TAGCCTGGTT ACTGTGGAGG 
GAAAGTCAGA AGCCACAAGG AGGGATCCTG GCGGATGATA TGGGCTTAGG AAAGACTCTG 
ACAATGATTG CACTCATCCT AACCAAGAAG AATCAGCAGA AAAGCAAGGA AAAGGAGAGA 
AGCGAGCCTG TGACGTGGCT TTCCAAGAAT GACTCCTCTG TCTTTACTTC CAGTGGAACA 
CTGATCGTCT GCCCCGCTTC CCTGATCCAT CACTGGAAGA ACGAAGTAGA GAAACGTGTA 
ACCAGCAACA GACTCAGAAT CTATCTCTAC CATGGGCCAA ACCGGAGTCG GCACGCAAAA 
GTACTCTCTA CGTATGACAT CGTGATTACT ACTTACAGCC TTCTGGCCAA GGAGATTCCT 
ACAACGAAGC AAGAGGGAGA GGTCCCAGGA GCAAACCTCA GTGTGGAGGG TACCTCAGCA 
CCTTTGCTTC AGGTAGTCTG GGCTCGGATC ATTTTGGATG AAGCTCATAA CGTGAAAAAC 
CCTCGAGTAC AGACATCCAT TGCTGTGTGT AAGCTCCAAG CCCAGGCCCG GTGGGCTGTC 
ACTGGAACCC CCATTCAGAA CAACCTGTTG GATATGTATT CACTGATGAA GTTTCTCCGT 
TGCTCCCCAT TTGATGAGTT CAGTCTTTGG AAGAGCCAGG TTGACAATGG TTCAATGAAA 
GGAGGGGAAC GGTTAAGCAT TTTAACCAAG AGCCTTTTGC TGAGGAGAAC AAAAGACCAG 
CTGGATTCCA CTGGTAAACC TCTGGTAGCA CTGCCTGCAC GTAGATGCCA GCTGCACCGG 
TTAAAGCTTT CTGAGGACGA GCGGGCCGTT TATGACATCT TTCTTGCAAG GTCAAGGTCA 
GCTCTCCAGT CCTATCTAAA GAGACAAGAA GGTAGAGGCA GCCACCATGG AAGAAGCCCT 
GATAATCCAT TCAGTAGAGT GGCTCAGGAG TTTGGATCCA GTGTGTCCCA GGGCTGCCCT 
GCGGCAGACT CCCAAAGACC CAGCACCGTC CATGTCCTGT CCCAGCTTCT GCGGCTTCGC 
CAGTGCTGCT GCCATCTTTC TCTGCTGAAG TCGGCTCTGG ACCCCACAGA ACTGGAGAGC 
GAAGGCCTTG TCCTGTCCCT GGAAGAACAG CTCAGTGCTC TGACCTTGTC CAAGGTCGAT 
GTCTCAGAGC CATCACCCAC GGTTAGCCTT AATGGCACAT GTTTCAAGGC GGAGCTTTTT 
GATGACACAC GAAGGAGCAC CAAGGTGTCT TCTCTGCTGG CAGAACTGGA GGCGATCCAA 
AAAGGCCCAG GGTCCCAAAA GAGTGTCATT GTCTCTCAGT GGACCAGCAT GCTGCAGGTA 
GTAGCACTGC ACCTCAAGAA GAACCGACTG ACTTACGCCA CCATCGATGG CTCTGTCAAC 
CCCAAACAGA GAATGGACCT GGTGGAGGCA TTTAACCACT CCCAGGGACC TCAGGTCATG 
CTGATCTCCC TCTTGGCTGG CGGTGTTGGA CTTAACCTGA CTGGAGGAAA CCACCTTTTC 
CTTCTGGACA TGCACTGGAA TCCCTCACTT GAAGACCAAG CTTGTGACCG GATTTACCGA 
GTAGGACAGA AGAAGGATGT TGTCATACAC AGGTTTGTCT GTGAGGGGAC AGTGGAAGAG 
AAGATCTTAC AGCTCCAAGA AAAAAAGAAA GATTTGGCAA AACAGGTCCT TTCTGGATCC 
GAAGGACCTG TCACCAAGCT CACACTGGCT GACCTCAAGA TCCTCTTTGG CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttf2 ( XM_006502149.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502212.1 Mus musculus transcription termination factor, RNA polymerase II (Ttf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu32338
Clone ID Related Accession (Same CDS sequence)	XM_006502149.1 , XM_006502149.2
Accession Version	XM_006502149.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	transcription termination factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502149.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG TTAAGTGTCC GGAGCACGGG GACGCCTGCT TCTTAAAGAC CGGCGTCCGC 
GATGGCCCGA ATAAGGGAAA GAGCTTCTAC GTGTGCCGGA CGAACACATG CGGCTTCGTG 
CAGGCCACCG ATATTCCTGT TTCTCACTGT TTATTGCATG AGGAGTTTGT GGTCGAGCTT 
CAGGGTTTGT TTCTACCACA GGACAAGAAA GAATGGAGGC TGTTCTTCCG ATGTGCTAGA 
ACCAAGGCGG AGGGGAAGCA GTGGTGTGGA AACGTCCCAT GGCGGCAGGA TCCTAACCCT 
AAAGAACTCT CTGTAACCAG TAAGCCTCAG CAGCCATCTG AGTCACAGCT TCACTCTCCC 
AGCCAGCCGA GAAACCCATT TAGGGTGCTT AACAAGAATC AAAAAACACT CGAACGAAAA 
CAGTTTGTCG AAGAAGGTGA GAGAAAGACA GCTGATAAGA AGCTGAGAGA AAACAATGAG 
CAGCTCTTGG ATCAAAGGAA AGAACAGAAG CCCAAATCTA ACAGCAGGAT GGAGAAGGAC 
CCCTCATCTG ACCTGGTGGC AACTAGACAG TCTGGAGGTG ACAGGGAAGA ACAAGAGAAA 
TCAAAGTTCC AGCCCAAAAC TAAGAAAGCT GAAGGAATGG CTTCCAAGCA AGGCCATGGA 
GAGGTGCTTC AAGGAATACC CAAGGGTCCT CACATGTCAG AGAGTGAGAG TCGAGGGGTC 
CCGAACAAGC CAGAGACTCT GAGAGAAAAG GAAACCCAGC TTTTGGTGCC AAGTGTTCCT 
GGTCAGAACC CAGAAAGCAA GGTCCAGAAA GAGGGACACG TCAGCAGGGA GCCTCTCCAG 
GGACCATCCA AGACTTGGAG GCCTGTTCCT GAAGCACCAG CAGCACCTGA GCTTTGCTCG 
GGAATGGCCC ACCATGCCAC CTCCAGCAGT GAGGACAGCG AAGATGATGG TGTTTCCAGT 
AGGCCGGGGT CCCCACTTCT CTTTGACTCG ACTGTGGACT CACAGAAGAA GGGGAGCCTT 
CAGCATTCTG ACCAGAGTGT GCAGAGACAG ATGCCTGCTG CTTCAGGTGT TTCCAAGAAG 
GGAGATTCCT CTGACCCAGC AGCCCAGCGT GCAAACCTAA CAACACAGCT GAAACAAAAG 
AAGGGTACCC TGGCTGCAGT GAACATCCAG GCTCTCCCTG ATAAGGGTGA GAAGTTGCTT 
AAGCAAATCC AGGCTTTGGA GGACGCACTC AGTGCTCTGG CTCTCTCCCC AGAACAAGGA 
ACTAAAGAGA AGTGCAGCGC TCAGGAACCA GAGCAGAGCA ACATCACCAA AGCTGCTGCT 
GCCCCTCTCC ACTTGGTACC TCCCCAGCCC CTTCCCAGGC CCCTCATCCA GCCTGCAAGC 
TCTCTAGGAC TAAAGGCAGG CCGCCAGGAA ACTCCTGAGG GAGCCAGCCA GTGCTCAGGA 
GGCCATATGA ACCAACATCA TCTTTATAAT GTGTGGAAAA TCACGAGTGA AGCCATAGAT 
GAGCTGCATC GGTCACTGAA GTCTTGTCCG GGTGAAACAG CAGTAGCAGA AGACCCAGCT 
GGCTTGAAGG TCCCCTTGCT ACTGCACCAG AAGCAGGCAT TAGCCTGGTT ACTGTGGAGG 
GAAAGTCAGA AGCCACAAGG AGGGATCCTG GCGGATGATA TGGGCTTAGG AAAGACTCTG 
ACAATGATTG CACTCATCCT AACCAAGAAG AATCAGCAGA AAAGCAAGGA AAAGGAGAGA 
AGCGAGCCTG TGACGTGGCT TTCCAAGAAT GACTCCTCTG TCTTTACTTC CAGTGGAACA 
CTGATCGTCT GCCCCGCTTC CCTGATCCAT CACTGGAAGA ACGAAGTAGA GAAACGTGTA 
ACCAGCAACA GACTCAGAAT CTATCTCTAC CATGGGCCAA ACCGGAGTCG GCACGCAAAA 
GTACTCTCTA CGTATGACAT CGTGATTACT ACTTACAGCC TTCTGGCCAA GGAGATTCCT 
ACAACGAAGC AAGAGGGAGA GGTCCCAGGA GCAAACCTCA GTGTGGAGGG TACCTCAGCA 
CCTTTGCTTC AGGTAGTCTG GGCTCGGATC ATTTTGGATG AAGCTCATAA CGTGAAAAAC 
CCTCGAGTAC AGACATCCAT TGCTGTGTGT AAGCTCCAAG CCCAGGCCCG GTGGGCTGTC 
ACTGGAACCC CCATTCAGAA CAACCTGTTG GATATGTATT CACTGATGAA GTTTCTCCGT 
TGCTCCCCAT TTGATGAGTT CAGTCTTTGG AAGAGCCAGG TTGACAATGG TTCAATGAAA 
GGAGGGGAAC GGTTAAGCAT TTTAACCAAG AGCCTTTTGC TGAGGAGAAC AAAAGACCAG 
CTGGATTCCA CTGGTAAACC TCTGGTAGCA CTGCCTGCAC GTAGATGCCA GCTGCACCGG 
TTAAAGCTTT CTGAGGACGA GCGGGCCGTT TATGACATCT TTCTTGCAAG GTCAAGGTCA 
GCTCTCCAGT CCTATCTAAA GAGACAAGAA GGTAGAGGCA GCCACCATGG AAGAAGCCCT 
GATAATCCAT TCAGTAGAGT GGCTCAGGAG TTTGGATCCA GTGTGTCCCA GGGCTGCCCT 
GCGGCAGACT CCCAAAGACC CAGCACCGTC CATGTCCTGT CCCAGCTTCT GCGGCTTCGC 
CAGTGCTGCT GCCATCTTTC TCTGCTGAAG TCGGCTCTGG ACCCCACAGA ACTGGAGAGC 
GAAGGCCTTG TCCTGTCCCT GGAAGAACAG CTCAGTGCTC TGACCTTGTC CAAGGTCGAT 
GTCTCAGAGC CATCACCCAC GGTTAGCCTT AATGGCACAT GTTTCAAGGC GGAGCTTTTT 
GATGACACAC GAAGGAGCAC CAAGGTGTCT TCTCTGCTGG CAGAACTGGA GGCGATCCAA 
AAAGGCCCAG GGTCCCAAAA GAGTGTCATT GTCTCTCAGT GGACCAGCAT GCTGCAGGTA 
GTAGCACTGC ACCTCAAGAA GAACCGACTG ACTTACGCCA CCATCGATGG CTCTGTCAAC 
CCCAAACAGA GAATGGACCT GGTGGAGGCA TTTAACCACT CCCAGGGACC TCAGGTCATG 
CTGATCTCCC TCTTGGCTGG CGGTGTTGGA CTTAACCTGA CTGGAGGAAA CCACCTTTTC 
CTTCTGGACA TGCACTGGAA TCCCTCACTT GAAGACCAAG CTTGTGACCG GATTTACCGA 
GTAGGACAGA AGAAGGATGT TGTCATACAC AGGTTTGTCT GTGAGGGGAC AGTGGAAGAG 
AAGATCTTAC AGCTCCAAGA AAAAAAGAAA GATTTGGCAA AACAGGTCCT TTCTGGATCC 
GAAGGACCTG TCACCAAGCT CACACTGGCT GACCTCAAGA TCCTCTTTGG CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502212.1
CDS	54..3410
Translation	MDLVKCPEHGDACFLKTGVRDGPNKGKSFYVCRTNTCGFVQATDIPVSHCLLHEEFVVELQGLFLPQDKKEWRLFFRCARTKAEGKQWCGNVPWRQDPNPKELSVTSKPQQPSESQLHSPSQPRNPFRVLNKNQKTLERKQFVEEGERKTADKKLRENNEQLLDQRKEQKPKSNSRMEKDPSSDLVATRQSGGDREEQEKSKFQPKTKKAEGMASKQGHGEVLQGIPKGPHMSESESRGVPNKPETLREKETQLLVPSVPGQNPESKVQKEGHVSREPLQGPSKTWRPVPEAPAAPELCSGMAHHATSSSEDSEDDGVSSRPGSPLLFDSTVDSQKKGSLQHSDQSVQRQMPAASGVSKKGDSSDPAAQRANLTTQLKQKKGTLAAVNIQALPDKGEKLLKQIQALEDALSALALSPEQGTKEKCSAQEPEQSNITKAAAAPLHLVPPQPLPRPLIQPASSLGLKAGRQETPEGASQCSGGHMNQHHLYNVWKITSEAIDELHRSLKSCPGETAVAEDPAGLKVPLLLHQKQALAWLLWRESQKPQGGILADDMGLGKTLTMIALILTKKNQQKSKEKERSEPVTWLSKNDSSVFTSSGTLIVCPASLIHHWKNEVEKRVTSNRLRIYLYHGPNRSRHAKVLSTYDIVITTYSLLAKEIPTTKQEGEVPGANLSVEGTSAPLLQVVWARIILDEAHNVKNPRVQTSIAVCKLQAQARWAVTGTPIQNNLLDMYSLMKFLRCSPFDEFSLWKSQVDNGSMKGGERLSILTKSLLLRRTKDQLDSTGKPLVALPARRCQLHRLKLSEDERAVYDIFLARSRSALQSYLKRQEGRGSHHGRSPDNPFSRVAQEFGSSVSQGCPAADSQRPSTVHVLSQLLRLRQCCCHLSLLKSALDPTELESEGLVLSLEEQLSALTLSKVDVSEPSPTVSLNGTCFKAELFDDTRRSTKVSSLLAELEAIQKGPGSQKSVIVSQWTSMLQVVALHLKKNRLTYATIDGSVNPKQRMDLVEAFNHSQGPQVMLISLLAGGVGLNLTGGNHLFLLDMHWNPSLEDQACDRIYRVGQKKDVVIHRFVCEGTVEEKILQLQEKKKDLAKQVLSGSEGPVTKLTLADLKILFGI

Target ORF information:

RefSeq Version	XM_006502149.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transcription termination factor, RNA polymerase II (Ttf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502149.2

ORF Insert Sequence:

ATGGACCTGG TTAAGTGTCC GGAGCACGGG GACGCCTGCT TCTTAAAGAC CGGCGTCCGC 
GATGGCCCGA ATAAGGGAAA GAGCTTCTAC GTGTGCCGGA CGAACACATG CGGCTTCGTG 
CAGGCCACCG ATATTCCTGT TTCTCACTGT TTATTGCATG AGGAGTTTGT GGTCGAGCTT 
CAGGGTTTGT TTCTACCACA GGACAAGAAA GAATGGAGGC TGTTCTTCCG ATGTGCTAGA 
ACCAAGGCGG AGGGGAAGCA GTGGTGTGGA AACGTCCCAT GGCGGCAGGA TCCTAACCCT 
AAAGAACTCT CTGTAACCAG TAAGCCTCAG CAGCCATCTG AGTCACAGCT TCACTCTCCC 
AGCCAGCCGA GAAACCCATT TAGGGTGCTT AACAAGAATC AAAAAACACT CGAACGAAAA 
CAGTTTGTCG AAGAAGGTGA GAGAAAGACA GCTGATAAGA AGCTGAGAGA AAACAATGAG 
CAGCTCTTGG ATCAAAGGAA AGAACAGAAG CCCAAATCTA ACAGCAGGAT GGAGAAGGAC 
CCCTCATCTG ACCTGGTGGC AACTAGACAG TCTGGAGGTG ACAGGGAAGA ACAAGAGAAA 
TCAAAGTTCC AGCCCAAAAC TAAGAAAGCT GAAGGAATGG CTTCCAAGCA AGGCCATGGA 
GAGGTGCTTC AAGGAATACC CAAGGGTCCT CACATGTCAG AGAGTGAGAG TCGAGGGGTC 
CCGAACAAGC CAGAGACTCT GAGAGAAAAG GAAACCCAGC TTTTGGTGCC AAGTGTTCCT 
GGTCAGAACC CAGAAAGCAA GGTCCAGAAA GAGGGACACG TCAGCAGGGA GCCTCTCCAG 
GGACCATCCA AGACTTGGAG GCCTGTTCCT GAAGCACCAG CAGCACCTGA GCTTTGCTCG 
GGAATGGCCC ACCATGCCAC CTCCAGCAGT GAGGACAGCG AAGATGATGG TGTTTCCAGT 
AGGCCGGGGT CCCCACTTCT CTTTGACTCG ACTGTGGACT CACAGAAGAA GGGGAGCCTT 
CAGCATTCTG ACCAGAGTGT GCAGAGACAG ATGCCTGCTG CTTCAGGTGT TTCCAAGAAG 
GGAGATTCCT CTGACCCAGC AGCCCAGCGT GCAAACCTAA CAACACAGCT GAAACAAAAG 
AAGGGTACCC TGGCTGCAGT GAACATCCAG GCTCTCCCTG ATAAGGGTGA GAAGTTGCTT 
AAGCAAATCC AGGCTTTGGA GGACGCACTC AGTGCTCTGG CTCTCTCCCC AGAACAAGGA 
ACTAAAGAGA AGTGCAGCGC TCAGGAACCA GAGCAGAGCA ACATCACCAA AGCTGCTGCT 
GCCCCTCTCC ACTTGGTACC TCCCCAGCCC CTTCCCAGGC CCCTCATCCA GCCTGCAAGC 
TCTCTAGGAC TAAAGGCAGG CCGCCAGGAA ACTCCTGAGG GAGCCAGCCA GTGCTCAGGA 
GGCCATATGA ACCAACATCA TCTTTATAAT GTGTGGAAAA TCACGAGTGA AGCCATAGAT 
GAGCTGCATC GGTCACTGAA GTCTTGTCCG GGTGAAACAG CAGTAGCAGA AGACCCAGCT 
GGCTTGAAGG TCCCCTTGCT ACTGCACCAG AAGCAGGCAT TAGCCTGGTT ACTGTGGAGG 
GAAAGTCAGA AGCCACAAGG AGGGATCCTG GCGGATGATA TGGGCTTAGG AAAGACTCTG 
ACAATGATTG CACTCATCCT AACCAAGAAG AATCAGCAGA AAAGCAAGGA AAAGGAGAGA 
AGCGAGCCTG TGACGTGGCT TTCCAAGAAT GACTCCTCTG TCTTTACTTC CAGTGGAACA 
CTGATCGTCT GCCCCGCTTC CCTGATCCAT CACTGGAAGA ACGAAGTAGA GAAACGTGTA 
ACCAGCAACA GACTCAGAAT CTATCTCTAC CATGGGCCAA ACCGGAGTCG GCACGCAAAA 
GTACTCTCTA CGTATGACAT CGTGATTACT ACTTACAGCC TTCTGGCCAA GGAGATTCCT 
ACAACGAAGC AAGAGGGAGA GGTCCCAGGA GCAAACCTCA GTGTGGAGGG TACCTCAGCA 
CCTTTGCTTC AGGTAGTCTG GGCTCGGATC ATTTTGGATG AAGCTCATAA CGTGAAAAAC 
CCTCGAGTAC AGACATCCAT TGCTGTGTGT AAGCTCCAAG CCCAGGCCCG GTGGGCTGTC 
ACTGGAACCC CCATTCAGAA CAACCTGTTG GATATGTATT CACTGATGAA GTTTCTCCGT 
TGCTCCCCAT TTGATGAGTT CAGTCTTTGG AAGAGCCAGG TTGACAATGG TTCAATGAAA 
GGAGGGGAAC GGTTAAGCAT TTTAACCAAG AGCCTTTTGC TGAGGAGAAC AAAAGACCAG 
CTGGATTCCA CTGGTAAACC TCTGGTAGCA CTGCCTGCAC GTAGATGCCA GCTGCACCGG 
TTAAAGCTTT CTGAGGACGA GCGGGCCGTT TATGACATCT TTCTTGCAAG GTCAAGGTCA 
GCTCTCCAGT CCTATCTAAA GAGACAAGAA GGTAGAGGCA GCCACCATGG AAGAAGCCCT 
GATAATCCAT TCAGTAGAGT GGCTCAGGAG TTTGGATCCA GTGTGTCCCA GGGCTGCCCT 
GCGGCAGACT CCCAAAGACC CAGCACCGTC CATGTCCTGT CCCAGCTTCT GCGGCTTCGC 
CAGTGCTGCT GCCATCTTTC TCTGCTGAAG TCGGCTCTGG ACCCCACAGA ACTGGAGAGC 
GAAGGCCTTG TCCTGTCCCT GGAAGAACAG CTCAGTGCTC TGACCTTGTC CAAGGTCGAT 
GTCTCAGAGC CATCACCCAC GGTTAGCCTT AATGGCACAT GTTTCAAGGC GGAGCTTTTT 
GATGACACAC GAAGGAGCAC CAAGGTGTCT TCTCTGCTGG CAGAACTGGA GGCGATCCAA 
AAAGGCCCAG GGTCCCAAAA GAGTGTCATT GTCTCTCAGT GGACCAGCAT GCTGCAGGTA 
GTAGCACTGC ACCTCAAGAA GAACCGACTG ACTTACGCCA CCATCGATGG CTCTGTCAAC 
CCCAAACAGA GAATGGACCT GGTGGAGGCA TTTAACCACT CCCAGGGACC TCAGGTCATG 
CTGATCTCCC TCTTGGCTGG CGGTGTTGGA CTTAACCTGA CTGGAGGAAA CCACCTTTTC 
CTTCTGGACA TGCACTGGAA TCCCTCACTT GAAGACCAAG CTTGTGACCG GATTTACCGA 
GTAGGACAGA AGAAGGATGT TGTCATACAC AGGTTTGTCT GTGAGGGGAC AGTGGAAGAG 
AAGATCTTAC AGCTCCAAGA AAAAAAGAAA GATTTGGCAA AACAGGTCCT TTCTGGATCC 
GAAGGACCTG TCACCAAGCT CACACTGGCT GACCTCAAGA TCCTCTTTGG CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ttf2 ( XM_006502149.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502212.1 Mus musculus transcription termination factor, RNA polymerase II (Ttf2), transcript variant X1, mRNA.

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CloneID	OMu32338
Clone ID Related Accession (Same CDS sequence)	XM_006502149.1 , XM_006502149.2
Accession Version	XM_006502149.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	transcription termination factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG TTAAGTGTCC GGAGCACGGG GACGCCTGCT TCTTAAAGAC CGGCGTCCGC 
GATGGCCCGA ATAAGGGAAA GAGCTTCTAC GTGTGCCGGA CGAACACATG CGGCTTCGTG 
CAGGCCACCG ATATTCCTGT TTCTCACTGT TTATTGCATG AGGAGTTTGT GGTCGAGCTT 
CAGGGTTTGT TTCTACCACA GGACAAGAAA GAATGGAGGC TGTTCTTCCG ATGTGCTAGA 
ACCAAGGCGG AGGGGAAGCA GTGGTGTGGA AACGTCCCAT GGCGGCAGGA TCCTAACCCT 
AAAGAACTCT CTGTAACCAG TAAGCCTCAG CAGCCATCTG AGTCACAGCT TCACTCTCCC 
AGCCAGCCGA GAAACCCATT TAGGGTGCTT AACAAGAATC AAAAAACACT CGAACGAAAA 
CAGTTTGTCG AAGAAGGTGA GAGAAAGACA GCTGATAAGA AGCTGAGAGA AAACAATGAG 
CAGCTCTTGG ATCAAAGGAA AGAACAGAAG CCCAAATCTA ACAGCAGGAT GGAGAAGGAC 
CCCTCATCTG ACCTGGTGGC AACTAGACAG TCTGGAGGTG ACAGGGAAGA ACAAGAGAAA 
TCAAAGTTCC AGCCCAAAAC TAAGAAAGCT GAAGGAATGG CTTCCAAGCA AGGCCATGGA 
GAGGTGCTTC AAGGAATACC CAAGGGTCCT CACATGTCAG AGAGTGAGAG TCGAGGGGTC 
CCGAACAAGC CAGAGACTCT GAGAGAAAAG GAAACCCAGC TTTTGGTGCC AAGTGTTCCT 
GGTCAGAACC CAGAAAGCAA GGTCCAGAAA GAGGGACACG TCAGCAGGGA GCCTCTCCAG 
GGACCATCCA AGACTTGGAG GCCTGTTCCT GAAGCACCAG CAGCACCTGA GCTTTGCTCG 
GGAATGGCCC ACCATGCCAC CTCCAGCAGT GAGGACAGCG AAGATGATGG TGTTTCCAGT 
AGGCCGGGGT CCCCACTTCT CTTTGACTCG ACTGTGGACT CACAGAAGAA GGGGAGCCTT 
CAGCATTCTG ACCAGAGTGT GCAGAGACAG ATGCCTGCTG CTTCAGGTGT TTCCAAGAAG 
GGAGATTCCT CTGACCCAGC AGCCCAGCGT GCAAACCTAA CAACACAGCT GAAACAAAAG 
AAGGGTACCC TGGCTGCAGT GAACATCCAG GCTCTCCCTG ATAAGGGTGA GAAGTTGCTT 
AAGCAAATCC AGGCTTTGGA GGACGCACTC AGTGCTCTGG CTCTCTCCCC AGAACAAGGA 
ACTAAAGAGA AGTGCAGCGC TCAGGAACCA GAGCAGAGCA ACATCACCAA AGCTGCTGCT 
GCCCCTCTCC ACTTGGTACC TCCCCAGCCC CTTCCCAGGC CCCTCATCCA GCCTGCAAGC 
TCTCTAGGAC TAAAGGCAGG CCGCCAGGAA ACTCCTGAGG GAGCCAGCCA GTGCTCAGGA 
GGCCATATGA ACCAACATCA TCTTTATAAT GTGTGGAAAA TCACGAGTGA AGCCATAGAT 
GAGCTGCATC GGTCACTGAA GTCTTGTCCG GGTGAAACAG CAGTAGCAGA AGACCCAGCT 
GGCTTGAAGG TCCCCTTGCT ACTGCACCAG AAGCAGGCAT TAGCCTGGTT ACTGTGGAGG 
GAAAGTCAGA AGCCACAAGG AGGGATCCTG GCGGATGATA TGGGCTTAGG AAAGACTCTG 
ACAATGATTG CACTCATCCT AACCAAGAAG AATCAGCAGA AAAGCAAGGA AAAGGAGAGA 
AGCGAGCCTG TGACGTGGCT TTCCAAGAAT GACTCCTCTG TCTTTACTTC CAGTGGAACA 
CTGATCGTCT GCCCCGCTTC CCTGATCCAT CACTGGAAGA ACGAAGTAGA GAAACGTGTA 
ACCAGCAACA GACTCAGAAT CTATCTCTAC CATGGGCCAA ACCGGAGTCG GCACGCAAAA 
GTACTCTCTA CGTATGACAT CGTGATTACT ACTTACAGCC TTCTGGCCAA GGAGATTCCT 
ACAACGAAGC AAGAGGGAGA GGTCCCAGGA GCAAACCTCA GTGTGGAGGG TACCTCAGCA 
CCTTTGCTTC AGGTAGTCTG GGCTCGGATC ATTTTGGATG AAGCTCATAA CGTGAAAAAC 
CCTCGAGTAC AGACATCCAT TGCTGTGTGT AAGCTCCAAG CCCAGGCCCG GTGGGCTGTC 
ACTGGAACCC CCATTCAGAA CAACCTGTTG GATATGTATT CACTGATGAA GTTTCTCCGT 
TGCTCCCCAT TTGATGAGTT CAGTCTTTGG AAGAGCCAGG TTGACAATGG TTCAATGAAA 
GGAGGGGAAC GGTTAAGCAT TTTAACCAAG AGCCTTTTGC TGAGGAGAAC AAAAGACCAG 
CTGGATTCCA CTGGTAAACC TCTGGTAGCA CTGCCTGCAC GTAGATGCCA GCTGCACCGG 
TTAAAGCTTT CTGAGGACGA GCGGGCCGTT TATGACATCT TTCTTGCAAG GTCAAGGTCA 
GCTCTCCAGT CCTATCTAAA GAGACAAGAA GGTAGAGGCA GCCACCATGG AAGAAGCCCT 
GATAATCCAT TCAGTAGAGT GGCTCAGGAG TTTGGATCCA GTGTGTCCCA GGGCTGCCCT 
GCGGCAGACT CCCAAAGACC CAGCACCGTC CATGTCCTGT CCCAGCTTCT GCGGCTTCGC 
CAGTGCTGCT GCCATCTTTC TCTGCTGAAG TCGGCTCTGG ACCCCACAGA ACTGGAGAGC 
GAAGGCCTTG TCCTGTCCCT GGAAGAACAG CTCAGTGCTC TGACCTTGTC CAAGGTCGAT 
GTCTCAGAGC CATCACCCAC GGTTAGCCTT AATGGCACAT GTTTCAAGGC GGAGCTTTTT 
GATGACACAC GAAGGAGCAC CAAGGTGTCT TCTCTGCTGG CAGAACTGGA GGCGATCCAA 
AAAGGCCCAG GGTCCCAAAA GAGTGTCATT GTCTCTCAGT GGACCAGCAT GCTGCAGGTA 
GTAGCACTGC ACCTCAAGAA GAACCGACTG ACTTACGCCA CCATCGATGG CTCTGTCAAC 
CCCAAACAGA GAATGGACCT GGTGGAGGCA TTTAACCACT CCCAGGGACC TCAGGTCATG 
CTGATCTCCC TCTTGGCTGG CGGTGTTGGA CTTAACCTGA CTGGAGGAAA CCACCTTTTC 
CTTCTGGACA TGCACTGGAA TCCCTCACTT GAAGACCAAG CTTGTGACCG GATTTACCGA 
GTAGGACAGA AGAAGGATGT TGTCATACAC AGGTTTGTCT GTGAGGGGAC AGTGGAAGAG 
AAGATCTTAC AGCTCCAAGA AAAAAAGAAA GATTTGGCAA AACAGGTCCT TTCTGGATCC 
GAAGGACCTG TCACCAAGCT CACACTGGCT GACCTCAAGA TCCTCTTTGG CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502212.1
CDS	79..3435
Translation	MDLVKCPEHGDACFLKTGVRDGPNKGKSFYVCRTNTCGFVQATDIPVSHCLLHEEFVVELQGLFLPQDKKEWRLFFRCARTKAEGKQWCGNVPWRQDPNPKELSVTSKPQQPSESQLHSPSQPRNPFRVLNKNQKTLERKQFVEEGERKTADKKLRENNEQLLDQRKEQKPKSNSRMEKDPSSDLVATRQSGGDREEQEKSKFQPKTKKAEGMASKQGHGEVLQGIPKGPHMSESESRGVPNKPETLREKETQLLVPSVPGQNPESKVQKEGHVSREPLQGPSKTWRPVPEAPAAPELCSGMAHHATSSSEDSEDDGVSSRPGSPLLFDSTVDSQKKGSLQHSDQSVQRQMPAASGVSKKGDSSDPAAQRANLTTQLKQKKGTLAAVNIQALPDKGEKLLKQIQALEDALSALALSPEQGTKEKCSAQEPEQSNITKAAAAPLHLVPPQPLPRPLIQPASSLGLKAGRQETPEGASQCSGGHMNQHHLYNVWKITSEAIDELHRSLKSCPGETAVAEDPAGLKVPLLLHQKQALAWLLWRESQKPQGGILADDMGLGKTLTMIALILTKKNQQKSKEKERSEPVTWLSKNDSSVFTSSGTLIVCPASLIHHWKNEVEKRVTSNRLRIYLYHGPNRSRHAKVLSTYDIVITTYSLLAKEIPTTKQEGEVPGANLSVEGTSAPLLQVVWARIILDEAHNVKNPRVQTSIAVCKLQAQARWAVTGTPIQNNLLDMYSLMKFLRCSPFDEFSLWKSQVDNGSMKGGERLSILTKSLLLRRTKDQLDSTGKPLVALPARRCQLHRLKLSEDERAVYDIFLARSRSALQSYLKRQEGRGSHHGRSPDNPFSRVAQEFGSSVSQGCPAADSQRPSTVHVLSQLLRLRQCCCHLSLLKSALDPTELESEGLVLSLEEQLSALTLSKVDVSEPSPTVSLNGTCFKAELFDDTRRSTKVSSLLAELEAIQKGPGSQKSVIVSQWTSMLQVVALHLKKNRLTYATIDGSVNPKQRMDLVEAFNHSQGPQVMLISLLAGGVGLNLTGGNHLFLLDMHWNPSLEDQACDRIYRVGQKKDVVIHRFVCEGTVEEKILQLQEKKKDLAKQVLSGSEGPVTKLTLADLKILFGI

Target ORF information:

RefSeq Version	XM_006502149.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transcription termination factor, RNA polymerase II (Ttf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502149.1

ORF Insert Sequence:

ATGGACCTGG TTAAGTGTCC GGAGCACGGG GACGCCTGCT TCTTAAAGAC CGGCGTCCGC 
GATGGCCCGA ATAAGGGAAA GAGCTTCTAC GTGTGCCGGA CGAACACATG CGGCTTCGTG 
CAGGCCACCG ATATTCCTGT TTCTCACTGT TTATTGCATG AGGAGTTTGT GGTCGAGCTT 
CAGGGTTTGT TTCTACCACA GGACAAGAAA GAATGGAGGC TGTTCTTCCG ATGTGCTAGA 
ACCAAGGCGG AGGGGAAGCA GTGGTGTGGA AACGTCCCAT GGCGGCAGGA TCCTAACCCT 
AAAGAACTCT CTGTAACCAG TAAGCCTCAG CAGCCATCTG AGTCACAGCT TCACTCTCCC 
AGCCAGCCGA GAAACCCATT TAGGGTGCTT AACAAGAATC AAAAAACACT CGAACGAAAA 
CAGTTTGTCG AAGAAGGTGA GAGAAAGACA GCTGATAAGA AGCTGAGAGA AAACAATGAG 
CAGCTCTTGG ATCAAAGGAA AGAACAGAAG CCCAAATCTA ACAGCAGGAT GGAGAAGGAC 
CCCTCATCTG ACCTGGTGGC AACTAGACAG TCTGGAGGTG ACAGGGAAGA ACAAGAGAAA 
TCAAAGTTCC AGCCCAAAAC TAAGAAAGCT GAAGGAATGG CTTCCAAGCA AGGCCATGGA 
GAGGTGCTTC AAGGAATACC CAAGGGTCCT CACATGTCAG AGAGTGAGAG TCGAGGGGTC 
CCGAACAAGC CAGAGACTCT GAGAGAAAAG GAAACCCAGC TTTTGGTGCC AAGTGTTCCT 
GGTCAGAACC CAGAAAGCAA GGTCCAGAAA GAGGGACACG TCAGCAGGGA GCCTCTCCAG 
GGACCATCCA AGACTTGGAG GCCTGTTCCT GAAGCACCAG CAGCACCTGA GCTTTGCTCG 
GGAATGGCCC ACCATGCCAC CTCCAGCAGT GAGGACAGCG AAGATGATGG TGTTTCCAGT 
AGGCCGGGGT CCCCACTTCT CTTTGACTCG ACTGTGGACT CACAGAAGAA GGGGAGCCTT 
CAGCATTCTG ACCAGAGTGT GCAGAGACAG ATGCCTGCTG CTTCAGGTGT TTCCAAGAAG 
GGAGATTCCT CTGACCCAGC AGCCCAGCGT GCAAACCTAA CAACACAGCT GAAACAAAAG 
AAGGGTACCC TGGCTGCAGT GAACATCCAG GCTCTCCCTG ATAAGGGTGA GAAGTTGCTT 
AAGCAAATCC AGGCTTTGGA GGACGCACTC AGTGCTCTGG CTCTCTCCCC AGAACAAGGA 
ACTAAAGAGA AGTGCAGCGC TCAGGAACCA GAGCAGAGCA ACATCACCAA AGCTGCTGCT 
GCCCCTCTCC ACTTGGTACC TCCCCAGCCC CTTCCCAGGC CCCTCATCCA GCCTGCAAGC 
TCTCTAGGAC TAAAGGCAGG CCGCCAGGAA ACTCCTGAGG GAGCCAGCCA GTGCTCAGGA 
GGCCATATGA ACCAACATCA TCTTTATAAT GTGTGGAAAA TCACGAGTGA AGCCATAGAT 
GAGCTGCATC GGTCACTGAA GTCTTGTCCG GGTGAAACAG CAGTAGCAGA AGACCCAGCT 
GGCTTGAAGG TCCCCTTGCT ACTGCACCAG AAGCAGGCAT TAGCCTGGTT ACTGTGGAGG 
GAAAGTCAGA AGCCACAAGG AGGGATCCTG GCGGATGATA TGGGCTTAGG AAAGACTCTG 
ACAATGATTG CACTCATCCT AACCAAGAAG AATCAGCAGA AAAGCAAGGA AAAGGAGAGA 
AGCGAGCCTG TGACGTGGCT TTCCAAGAAT GACTCCTCTG TCTTTACTTC CAGTGGAACA 
CTGATCGTCT GCCCCGCTTC CCTGATCCAT CACTGGAAGA ACGAAGTAGA GAAACGTGTA 
ACCAGCAACA GACTCAGAAT CTATCTCTAC CATGGGCCAA ACCGGAGTCG GCACGCAAAA 
GTACTCTCTA CGTATGACAT CGTGATTACT ACTTACAGCC TTCTGGCCAA GGAGATTCCT 
ACAACGAAGC AAGAGGGAGA GGTCCCAGGA GCAAACCTCA GTGTGGAGGG TACCTCAGCA 
CCTTTGCTTC AGGTAGTCTG GGCTCGGATC ATTTTGGATG AAGCTCATAA CGTGAAAAAC 
CCTCGAGTAC AGACATCCAT TGCTGTGTGT AAGCTCCAAG CCCAGGCCCG GTGGGCTGTC 
ACTGGAACCC CCATTCAGAA CAACCTGTTG GATATGTATT CACTGATGAA GTTTCTCCGT 
TGCTCCCCAT TTGATGAGTT CAGTCTTTGG AAGAGCCAGG TTGACAATGG TTCAATGAAA 
GGAGGGGAAC GGTTAAGCAT TTTAACCAAG AGCCTTTTGC TGAGGAGAAC AAAAGACCAG 
CTGGATTCCA CTGGTAAACC TCTGGTAGCA CTGCCTGCAC GTAGATGCCA GCTGCACCGG 
TTAAAGCTTT CTGAGGACGA GCGGGCCGTT TATGACATCT TTCTTGCAAG GTCAAGGTCA 
GCTCTCCAGT CCTATCTAAA GAGACAAGAA GGTAGAGGCA GCCACCATGG AAGAAGCCCT 
GATAATCCAT TCAGTAGAGT GGCTCAGGAG TTTGGATCCA GTGTGTCCCA GGGCTGCCCT 
GCGGCAGACT CCCAAAGACC CAGCACCGTC CATGTCCTGT CCCAGCTTCT GCGGCTTCGC 
CAGTGCTGCT GCCATCTTTC TCTGCTGAAG TCGGCTCTGG ACCCCACAGA ACTGGAGAGC 
GAAGGCCTTG TCCTGTCCCT GGAAGAACAG CTCAGTGCTC TGACCTTGTC CAAGGTCGAT 
GTCTCAGAGC CATCACCCAC GGTTAGCCTT AATGGCACAT GTTTCAAGGC GGAGCTTTTT 
GATGACACAC GAAGGAGCAC CAAGGTGTCT TCTCTGCTGG CAGAACTGGA GGCGATCCAA 
AAAGGCCCAG GGTCCCAAAA GAGTGTCATT GTCTCTCAGT GGACCAGCAT GCTGCAGGTA 
GTAGCACTGC ACCTCAAGAA GAACCGACTG ACTTACGCCA CCATCGATGG CTCTGTCAAC 
CCCAAACAGA GAATGGACCT GGTGGAGGCA TTTAACCACT CCCAGGGACC TCAGGTCATG 
CTGATCTCCC TCTTGGCTGG CGGTGTTGGA CTTAACCTGA CTGGAGGAAA CCACCTTTTC 
CTTCTGGACA TGCACTGGAA TCCCTCACTT GAAGACCAAG CTTGTGACCG GATTTACCGA 
GTAGGACAGA AGAAGGATGT TGTCATACAC AGGTTTGTCT GTGAGGGGAC AGTGGAAGAG 
AAGATCTTAC AGCTCCAAGA AAAAAAGAAA GATTTGGCAA AACAGGTCCT TTCTGGATCC 
GAAGGACCTG TCACCAAGCT CACACTGGCT GACCTCAAGA TCCTCTTTGG CATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of mouse TTF-2 gene causes cleft palate.
Journal of cellular and molecular medicine16(10)2362-8(2012 Oct)
Meng T,Shi JY,Wu M,Wang Y,Li L,Liu Y,Zheng Q,Huang L,Shi B

Embryonic epithelial Pten deletion through Nkx2.1-cre leads to thyroid tumorigenesis in a strain-dependent manner.
Endocrine-related cancer19(2)111-122(2012 Apr)
Tiozzo C,Danopoulos S,Lavarreda-Pearce M,Baptista S,Varimezova R,Al Alam D,Warburton D,Virender R,De Langhe S,Di Cristofano A,Bellusci S,Minoo P

Cytochrome P450 2E1-dependent oxidant stress and upregulation of anti-oxidant defense in liver cells.
Journal of gastroenterology and hepatology21 Suppl 3S22-5(2006 Oct)
Cederbaum AI

Identification of a structurally distinct CD101 molecule encoded in the 950-kb Idd10 region of NOD mice.
Diabetes52(6)1551-6(2003 Jun)
Penha-Gon?alves C,Moule C,Smink LJ,Howson J,Gregory S,Rogers J,Lyons PA,Suttie JJ,Lord CJ,Peterson LB,Todd JA,Wicker LS

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Ttf2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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