Epn3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Epn3
Entrez Gene ID	71889
Full Name	epsin 3
Synonyms	2310022G12Rik
General protein information	Preferred Names epsin 3 Names epsin-3 EPS-15-interacting protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 D

mRNA and Protein(s)

mRNA	Protein	Name
NM_001361558.1	NP_001348487.1	epsin-3 isoform 2
XM_006534266.2	XP_006534329.1	epsin-3 isoform X1
NM_027984.3	NP_082260.1	epsin-3 isoform 1
XM_006534267.2	XP_006534330.1	epsin-3 isoform X2
XM_006534266.3	XP_006534329.1	epsin-3 isoform X1
XM_006534266.4	XP_006534329.1	epsin-3 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Epn3	NP_082260.1
Gallus gallus (chicken)	EPN3	XP_003642413.2
Canis lupus familiaris (dog)	EPN3	XP_005624698.1
Rattus norvegicus (Norway rat)	Epn3	NP_001019962.1
Pan troglodytes (chimpanzee)	EPN3	XP_003315664.1
Bos taurus (cattle)	EPN3	NP_001039899.1
Danio rerio (zebrafish)	LOC794862	XP_005164437.1
Homo sapiens (human)	EPN3	NP_060427.2
Danio rerio (zebrafish)	LOC571453	XP_700135.5
Xenopus tropicalis (tropical clawed frog)	epn3	NP_001005693.1

Gene Ontology
Bibliography

Related articles in PubMed

EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis.
Mori J, Tanikawa C, Ohnishi N, Funauchi Y, Toyoshima O, Ueda K, Matsuda K
Neoplasia (New York, N.Y.)19(3)185-195(2017 Mar)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits.
Messa M, Fern?ndez-Busnadiego R, Sun EW, Chen H, Czapla H, Wrasman K, Wu Y, Ko G, Ross T, Wendland B, De Camilli P
eLife3e03311(2014 Aug)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Selective high-level expression of epsin 3 in gastric parietal cells, where it is localized at endocytic sites of apical canaliculi.
Ko G, Paradise S, Chen H, Graham M, Vecchi M, Bianchi F, Cremona O, Di Fiore PP, De Camilli P
Proceedings of the National Academy of Sciences of the United States of America107(50)21511-6(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits.
Title: Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits.

data suggest that epsin 3 cooperates with other bilayer binding proteins with curvature sensing/generating properties in the specialized traffic and membrane remodeling processes typical of gastric parietal cells
Title: Selective high-level expression of epsin 3 in gastric parietal cells, where it is localized at endocytic sites of apical canaliculi.

The following Epn3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Epn3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu46961	NM_001361558.1 Latest version!	Mus musculus epsin 3 (Epn3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu46961	XM_006534267.2 Latest version!	Mus musculus epsin 3 (Epn3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu04899	NM_027984.3 Latest version!	Mus musculus epsin 3 (Epn3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu04899	XM_006534266.4 Latest version!	Mus musculus epsin 3 (Epn3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu04899	XM_006534266.2	Mus musculus epsin 3 (Epn3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu04899	XM_006534266.3	Mus musculus epsin 3 (Epn3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Epn3 ( NM_001361558.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001348487.1 Mus musculus epsin 3 (Epn3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu46961
Clone ID Related Accession (Same CDS sequence)	NM_001361558.1 , XM_006534267.2
Accession Version	NM_001361558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Mus musculus(house mouse)
Product	epsin-3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645809.8. On Mar 29, 2018 this sequence version replaced XM_006534267.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK158031.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
GGGGTGAGAT CCTGGAAGGG AGATGACTCT CCAGTGGCCA ACGGCGCAGA ACCTGCTGGC 
CAACGTCGTC AACGGGACAG GGAGCCTGAG AGAGAAGAGA GAAAGGAGGA GGAGAAGCTG 
AAAACTAGTC AGTCCTCCAT CCTGGACTTG GCTGACATCT TCGCACCTGC CCCGGCCCTG 
CCTTCCACAC ACTGCTCTGC TGACCCATGG GACATCCCAG GTCTCAGGCC GAACACAGAG 
CCAAGTGGCT CCTCTTGGGG ACCTTCTGCA GACCCCTGGT CTCCAGTTCC CTCTGGAAAT 
GCCCTGTCCC GAAGCCAGCC TTGGGATTTG CTTCCTACTC TTTCTTCCTC TGAGCCCTGG 
GGCAGGACCC CAGTGCTACC TTCTGGACCC CCCATCGCAG ACCCCTGGGC ACCAAGCTCC 
CCTACCCGCA AACTCCCCAG CACTGGAGCA GACCCTTGGG GGGCCTCTAT GGAGACCTCT 
GACACCTCTG CTCTAGGTGG TGCCTCGCCC TTCGACCCAT TTGCCAAACC TCTAGAATCC 
ACAGAACCCA AGGAGAGCCG GGACAGTGCC CAGGCCCTGC CCACGGGGAA GTCCCCCAGC 
ACTGTGGAGC TAGACCCATT TGGAGACTCC AGCCCCAGTT GCAAGCAGAA TGGCATGAAG 
GAACCTGAAG CCCTTGACCT GGGTGTGCTA GGAGAGGCGC TACCGCAGCA GCCAGGAAAG 
GAAGCGCGCC CCTGCCGGAC TCCGGAGTCC TTCCTGGGCC CCTCTGCCTC TTCTTTGGTC 
AACTTGGACT CACTAGTCAA GGCACCCCTG GCTGCAAGGA CCCGGAATCC CTTCCTGACA 
GGTCTGGGTG TTCCGTCCCC AACCAACCCG TTTGGCGCGG GCGATCAGGG CAGGCCGACC 
CTAAACCAGA TGCGTACCGG CTCCCCTGCT CTGGGCCTGC CGCCGGGGGG ACCCGTCGGG 
GCGCCCGTGG GCTCTATGAC CTACAGCGCC TCTCTGCCCC TTCCGCTCAG CAGCGTGCCG 
GTAGGCGCGA CCCTCCCCGC CTCGGTCAGC GTCTTCCCGC AAGCGGGAGC CTTCGCCCCG 
CCGCCGGCGA GCTTGCCTCA GCCACTGCTG CCCACGTCCG GCCCAATGGG GCCGCTGCCC 
CCGCAGGCTG GCACTAATCC CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001348487.1
CDS	422..2251
Translation	MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPPSSLMSEIADLTFNTVAFAEVMGMVWRRLNDSGKNWRHVYKALTLLDYLLKTGSERVAHQCRENLYTIQTLKDFQYIDRDGKDQGVNVREKVKQVMALLKDEERLRQERTHALKTKERMALEGMGIGSGQLGYSRRSRGSPSSYTSASSSPRYASDLEQARPQTSGEEELQLQLALAMSREEAERGVRSWKGDDSPVANGAEPAGQRRQRDREPEREERKEEEKLKTSQSSILDLADIFAPAPALPSTHCSADPWDIPGLRPNTEPSGSSWGPSADPWSPVPSGNALSRSQPWDLLPTLSSSEPWGRTPVLPSGPPIADPWAPSSPTRKLPSTGADPWGASMETSDTSALGGASPFDPFAKPLESTEPKESRDSAQALPTGKSPSTVELDPFGDSSPSCKQNGMKEPEALDLGVLGEALPQQPGKEARPCRTPESFLGPSASSLVNLDSLVKAPLAARTRNPFLTGLGVPSPTNPFGAGDQGRPTLNQMRTGSPALGLPPGGPVGAPVGSMTYSASLPLPLSSVPVGATLPASVSVFPQAGAFAPPPASLPQPLLPTSGPMGPLPPQAGTNPFL

Target ORF information:

RefSeq Version	NM_001361558.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus epsin 3 (Epn3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001361558.1

ORF Insert Sequence:

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
GGGGTGAGAT CCTGGAAGGG AGATGACTCT CCAGTGGCCA ACGGCGCAGA ACCTGCTGGC 
CAACGTCGTC AACGGGACAG GGAGCCTGAG AGAGAAGAGA GAAAGGAGGA GGAGAAGCTG 
AAAACTAGTC AGTCCTCCAT CCTGGACTTG GCTGACATCT TCGCACCTGC CCCGGCCCTG 
CCTTCCACAC ACTGCTCTGC TGACCCATGG GACATCCCAG GTCTCAGGCC GAACACAGAG 
CCAAGTGGCT CCTCTTGGGG ACCTTCTGCA GACCCCTGGT CTCCAGTTCC CTCTGGAAAT 
GCCCTGTCCC GAAGCCAGCC TTGGGATTTG CTTCCTACTC TTTCTTCCTC TGAGCCCTGG 
GGCAGGACCC CAGTGCTACC TTCTGGACCC CCCATCGCAG ACCCCTGGGC ACCAAGCTCC 
CCTACCCGCA AACTCCCCAG CACTGGAGCA GACCCTTGGG GGGCCTCTAT GGAGACCTCT 
GACACCTCTG CTCTAGGTGG TGCCTCGCCC TTCGACCCAT TTGCCAAACC TCTAGAATCC 
ACAGAACCCA AGGAGAGCCG GGACAGTGCC CAGGCCCTGC CCACGGGGAA GTCCCCCAGC 
ACTGTGGAGC TAGACCCATT TGGAGACTCC AGCCCCAGTT GCAAGCAGAA TGGCATGAAG 
GAACCTGAAG CCCTTGACCT GGGTGTGCTA GGAGAGGCGC TACCGCAGCA GCCAGGAAAG 
GAAGCGCGCC CCTGCCGGAC TCCGGAGTCC TTCCTGGGCC CCTCTGCCTC TTCTTTGGTC 
AACTTGGACT CACTAGTCAA GGCACCCCTG GCTGCAAGGA CCCGGAATCC CTTCCTGACA 
GGTCTGGGTG TTCCGTCCCC AACCAACCCG TTTGGCGCGG GCGATCAGGG CAGGCCGACC 
CTAAACCAGA TGCGTACCGG CTCCCCTGCT CTGGGCCTGC CGCCGGGGGG ACCCGTCGGG 
GCGCCCGTGG GCTCTATGAC CTACAGCGCC TCTCTGCCCC TTCCGCTCAG CAGCGTGCCG 
GTAGGCGCGA CCCTCCCCGC CTCGGTCAGC GTCTTCCCGC AAGCGGGAGC CTTCGCCCCG 
CCGCCGGCGA GCTTGCCTCA GCCACTGCTG CCCACGTCCG GCCCAATGGG GCCGCTGCCC 
CCGCAGGCTG GCACTAATCC CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Epn3 ( XM_006534267.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534330.1 Mus musculus epsin 3 (Epn3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu46961
Clone ID Related Accession (Same CDS sequence)	NM_001361558.1 , XM_006534267.2
Accession Version	XM_006534267.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	epsin-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
GGGGTGAGAT CCTGGAAGGG AGATGACTCT CCAGTGGCCA ACGGCGCAGA ACCTGCTGGC 
CAACGTCGTC AACGGGACAG GGAGCCTGAG AGAGAAGAGA GAAAGGAGGA GGAGAAGCTG 
AAAACTAGTC AGTCCTCCAT CCTGGACTTG GCTGACATCT TCGCACCTGC CCCGGCCCTG 
CCTTCCACAC ACTGCTCTGC TGACCCATGG GACATCCCAG GTCTCAGGCC GAACACAGAG 
CCAAGTGGCT CCTCTTGGGG ACCTTCTGCA GACCCCTGGT CTCCAGTTCC CTCTGGAAAT 
GCCCTGTCCC GAAGCCAGCC TTGGGATTTG CTTCCTACTC TTTCTTCCTC TGAGCCCTGG 
GGCAGGACCC CAGTGCTACC TTCTGGACCC CCCATCGCAG ACCCCTGGGC ACCAAGCTCC 
CCTACCCGCA AACTCCCCAG CACTGGAGCA GACCCTTGGG GGGCCTCTAT GGAGACCTCT 
GACACCTCTG CTCTAGGTGG TGCCTCGCCC TTCGACCCAT TTGCCAAACC TCTAGAATCC 
ACAGAACCCA AGGAGAGCCG GGACAGTGCC CAGGCCCTGC CCACGGGGAA GTCCCCCAGC 
ACTGTGGAGC TAGACCCATT TGGAGACTCC AGCCCCAGTT GCAAGCAGAA TGGCATGAAG 
GAACCTGAAG CCCTTGACCT GGGTGTGCTA GGAGAGGCGC TACCGCAGCA GCCAGGAAAG 
GAAGCGCGCC CCTGCCGGAC TCCGGAGTCC TTCCTGGGCC CCTCTGCCTC TTCTTTGGTC 
AACTTGGACT CACTAGTCAA GGCACCCCTG GCTGCAAGGA CCCGGAATCC CTTCCTGACA 
GGTCTGGGTG TTCCGTCCCC AACCAACCCG TTTGGCGCGG GCGATCAGGG CAGGCCGACC 
CTAAACCAGA TGCGTACCGG CTCCCCTGCT CTGGGCCTGC CGCCGGGGGG ACCCGTCGGG 
GCGCCCGTGG GCTCTATGAC CTACAGCGCC TCTCTGCCCC TTCCGCTCAG CAGCGTGCCG 
GTAGGCGCGA CCCTCCCCGC CTCGGTCAGC GTCTTCCCGC AAGCGGGAGC CTTCGCCCCG 
CCGCCGGCGA GCTTGCCTCA GCCACTGCTG CCCACGTCCG GCCCAATGGG GCCGCTGCCC 
CCGCAGGCTG GCACTAATCC CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534330.1
CDS	417..2246
Translation	MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPPSSLMSEIADLTFNTVAFAEVMGMVWRRLNDSGKNWRHVYKALTLLDYLLKTGSERVAHQCRENLYTIQTLKDFQYIDRDGKDQGVNVREKVKQVMALLKDEERLRQERTHALKTKERMALEGMGIGSGQLGYSRRSRGSPSSYTSASSSPRYASDLEQARPQTSGEEELQLQLALAMSREEAERGVRSWKGDDSPVANGAEPAGQRRQRDREPEREERKEEEKLKTSQSSILDLADIFAPAPALPSTHCSADPWDIPGLRPNTEPSGSSWGPSADPWSPVPSGNALSRSQPWDLLPTLSSSEPWGRTPVLPSGPPIADPWAPSSPTRKLPSTGADPWGASMETSDTSALGGASPFDPFAKPLESTEPKESRDSAQALPTGKSPSTVELDPFGDSSPSCKQNGMKEPEALDLGVLGEALPQQPGKEARPCRTPESFLGPSASSLVNLDSLVKAPLAARTRNPFLTGLGVPSPTNPFGAGDQGRPTLNQMRTGSPALGLPPGGPVGAPVGSMTYSASLPLPLSSVPVGATLPASVSVFPQAGAFAPPPASLPQPLLPTSGPMGPLPPQAGTNPFL

Target ORF information:

RefSeq Version	XM_006534267.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus epsin 3 (Epn3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534267.2

ORF Insert Sequence:

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
GGGGTGAGAT CCTGGAAGGG AGATGACTCT CCAGTGGCCA ACGGCGCAGA ACCTGCTGGC 
CAACGTCGTC AACGGGACAG GGAGCCTGAG AGAGAAGAGA GAAAGGAGGA GGAGAAGCTG 
AAAACTAGTC AGTCCTCCAT CCTGGACTTG GCTGACATCT TCGCACCTGC CCCGGCCCTG 
CCTTCCACAC ACTGCTCTGC TGACCCATGG GACATCCCAG GTCTCAGGCC GAACACAGAG 
CCAAGTGGCT CCTCTTGGGG ACCTTCTGCA GACCCCTGGT CTCCAGTTCC CTCTGGAAAT 
GCCCTGTCCC GAAGCCAGCC TTGGGATTTG CTTCCTACTC TTTCTTCCTC TGAGCCCTGG 
GGCAGGACCC CAGTGCTACC TTCTGGACCC CCCATCGCAG ACCCCTGGGC ACCAAGCTCC 
CCTACCCGCA AACTCCCCAG CACTGGAGCA GACCCTTGGG GGGCCTCTAT GGAGACCTCT 
GACACCTCTG CTCTAGGTGG TGCCTCGCCC TTCGACCCAT TTGCCAAACC TCTAGAATCC 
ACAGAACCCA AGGAGAGCCG GGACAGTGCC CAGGCCCTGC CCACGGGGAA GTCCCCCAGC 
ACTGTGGAGC TAGACCCATT TGGAGACTCC AGCCCCAGTT GCAAGCAGAA TGGCATGAAG 
GAACCTGAAG CCCTTGACCT GGGTGTGCTA GGAGAGGCGC TACCGCAGCA GCCAGGAAAG 
GAAGCGCGCC CCTGCCGGAC TCCGGAGTCC TTCCTGGGCC CCTCTGCCTC TTCTTTGGTC 
AACTTGGACT CACTAGTCAA GGCACCCCTG GCTGCAAGGA CCCGGAATCC CTTCCTGACA 
GGTCTGGGTG TTCCGTCCCC AACCAACCCG TTTGGCGCGG GCGATCAGGG CAGGCCGACC 
CTAAACCAGA TGCGTACCGG CTCCCCTGCT CTGGGCCTGC CGCCGGGGGG ACCCGTCGGG 
GCGCCCGTGG GCTCTATGAC CTACAGCGCC TCTCTGCCCC TTCCGCTCAG CAGCGTGCCG 
GTAGGCGCGA CCCTCCCCGC CTCGGTCAGC GTCTTCCCGC AAGCGGGAGC CTTCGCCCCG 
CCGCCGGCGA GCTTGCCTCA GCCACTGCTG CCCACGTCCG GCCCAATGGG GCCGCTGCCC 
CCGCAGGCTG GCACTAATCC CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Epn3 ( NM_027984.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082260.1 Mus musculus epsin 3 (Epn3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu04899
Clone ID Related Accession (Same CDS sequence)	XM_006534266.2 , NM_027984.3 , XM_006534266.3 , XM_006534266.4
Accession Version	NM_027984.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	epsin-3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL645809.8 and AK009469.1. On May 1, 2010 this sequence version replaced NM_027984.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC016454.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
CCAGTCCCCC CAGCCTCCCA CAGGGATGAG GACCTGCAGC TGCAGCTGGC TCTGAGCCTG 
AGCCGGCAAG AGCATGAGAA GGGGGTGAGA TCCTGGAAGG GAGATGACTC TCCAGTGGCC 
AACGGCGCAG AACCTGCTGG CCAACGTCGT CAACGGGACA GGGAGCCTGA GAGAGAAGAG 
AGAAAGGAGG AGGAGAAGCT GAAAACTAGT CAGTCCTCCA TCCTGGACTT GGCTGACATC 
TTCGCACCTG CCCCGGCCCT GCCTTCCACA CACTGCTCTG CTGACCCATG GGACATCCCA 
GGTCTCAGGC CGAACACAGA GCCAAGTGGC TCCTCTTGGG GACCTTCTGC AGACCCCTGG 
TCTCCAGTTC CCTCTGGAAA TGCCCTGTCC CGAAGCCAGC CTTGGGATTT GCTTCCTACT 
CTTTCTTCCT CTGAGCCCTG GGGCAGGACC CCAGTGCTAC CTTCTGGACC CCCCATCGCA 
GACCCCTGGG CACCAAGCTC CCCTACCCGC AAACTCCCCA GCACTGGAGC AGACCCTTGG 
GGGGCCTCTA TGGAGACCTC TGACACCTCT GCTCTAGGTG GTGCCTCGCC CTTCGACCCA 
TTTGCCAAAC CTCTAGAATC CACAGAACCC AAGGAGAGCC GGGACAGTGC CCAGGCCCTG 
CCCACGGGGA AGTCCCCCAG CACTGTGGAG CTAGACCCAT TTGGAGACTC CAGCCCCAGT 
TGCAAGCAGA ATGGCATGAA GGAACCTGAA GCCCTTGACC TGGGTGTGCT AGGAGAGGCG 
CTACCGCAGC AGCCAGGAAA GGAAGCGCGC CCCTGCCGGA CTCCGGAGTC CTTCCTGGGC 
CCCTCTGCCT CTTCTTTGGT CAACTTGGAC TCACTAGTCA AGGCACCCCT GGCTGCAAGG 
ACCCGGAATC CCTTCCTGAC AGGTCTGGGT GTTCCGTCCC CAACCAACCC GTTTGGCGCG 
GGCGATCAGG GCAGGCCGAC CCTAAACCAG ATGCGTACCG GCTCCCCTGC TCTGGGCCTG 
CCGCCGGGGG GACCCGTCGG GGCGCCCGTG GGCTCTATGA CCTACAGCGC CTCTCTGCCC 
CTTCCGCTCA GCAGCGTGCC GGTAGGCGCG ACCCTCCCCG CCTCGGTCAG CGTCTTCCCG 
CAAGCGGGAG CCTTCGCCCC GCCGCCGGCG AGCTTGCCTC AGCCACTGCT GCCCACGTCC 
GGCCCAATGG GGCCGCTGCC CCCGCAGGCT GGCACTAATC CCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082260.1
CDS	422..2332
Translation	MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPPSSLMSEIADLTFNTVAFAEVMGMVWRRLNDSGKNWRHVYKALTLLDYLLKTGSERVAHQCRENLYTIQTLKDFQYIDRDGKDQGVNVREKVKQVMALLKDEERLRQERTHALKTKERMALEGMGIGSGQLGYSRRSRGSPSSYTSASSSPRYASDLEQARPQTSGEEELQLQLALAMSREEAERPVPPASHRDEDLQLQLALSLSRQEHEKGVRSWKGDDSPVANGAEPAGQRRQRDREPEREERKEEEKLKTSQSSILDLADIFAPAPALPSTHCSADPWDIPGLRPNTEPSGSSWGPSADPWSPVPSGNALSRSQPWDLLPTLSSSEPWGRTPVLPSGPPIADPWAPSSPTRKLPSTGADPWGASMETSDTSALGGASPFDPFAKPLESTEPKESRDSAQALPTGKSPSTVELDPFGDSSPSCKQNGMKEPEALDLGVLGEALPQQPGKEARPCRTPESFLGPSASSLVNLDSLVKAPLAARTRNPFLTGLGVPSPTNPFGAGDQGRPTLNQMRTGSPALGLPPGGPVGAPVGSMTYSASLPLPLSSVPVGATLPASVSVFPQAGAFAPPPASLPQPLLPTSGPMGPLPPQAGTNPFL

Target ORF information:

RefSeq Version	NM_027984.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus epsin 3 (Epn3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027984.3

ORF Insert Sequence:

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
CCAGTCCCCC CAGCCTCCCA CAGGGATGAG GACCTGCAGC TGCAGCTGGC TCTGAGCCTG 
AGCCGGCAAG AGCATGAGAA GGGGGTGAGA TCCTGGAAGG GAGATGACTC TCCAGTGGCC 
AACGGCGCAG AACCTGCTGG CCAACGTCGT CAACGGGACA GGGAGCCTGA GAGAGAAGAG 
AGAAAGGAGG AGGAGAAGCT GAAAACTAGT CAGTCCTCCA TCCTGGACTT GGCTGACATC 
TTCGCACCTG CCCCGGCCCT GCCTTCCACA CACTGCTCTG CTGACCCATG GGACATCCCA 
GGTCTCAGGC CGAACACAGA GCCAAGTGGC TCCTCTTGGG GACCTTCTGC AGACCCCTGG 
TCTCCAGTTC CCTCTGGAAA TGCCCTGTCC CGAAGCCAGC CTTGGGATTT GCTTCCTACT 
CTTTCTTCCT CTGAGCCCTG GGGCAGGACC CCAGTGCTAC CTTCTGGACC CCCCATCGCA 
GACCCCTGGG CACCAAGCTC CCCTACCCGC AAACTCCCCA GCACTGGAGC AGACCCTTGG 
GGGGCCTCTA TGGAGACCTC TGACACCTCT GCTCTAGGTG GTGCCTCGCC CTTCGACCCA 
TTTGCCAAAC CTCTAGAATC CACAGAACCC AAGGAGAGCC GGGACAGTGC CCAGGCCCTG 
CCCACGGGGA AGTCCCCCAG CACTGTGGAG CTAGACCCAT TTGGAGACTC CAGCCCCAGT 
TGCAAGCAGA ATGGCATGAA GGAACCTGAA GCCCTTGACC TGGGTGTGCT AGGAGAGGCG 
CTACCGCAGC AGCCAGGAAA GGAAGCGCGC CCCTGCCGGA CTCCGGAGTC CTTCCTGGGC 
CCCTCTGCCT CTTCTTTGGT CAACTTGGAC TCACTAGTCA AGGCACCCCT GGCTGCAAGG 
ACCCGGAATC CCTTCCTGAC AGGTCTGGGT GTTCCGTCCC CAACCAACCC GTTTGGCGCG 
GGCGATCAGG GCAGGCCGAC CCTAAACCAG ATGCGTACCG GCTCCCCTGC TCTGGGCCTG 
CCGCCGGGGG GACCCGTCGG GGCGCCCGTG GGCTCTATGA CCTACAGCGC CTCTCTGCCC 
CTTCCGCTCA GCAGCGTGCC GGTAGGCGCG ACCCTCCCCG CCTCGGTCAG CGTCTTCCCG 
CAAGCGGGAG CCTTCGCCCC GCCGCCGGCG AGCTTGCCTC AGCCACTGCT GCCCACGTCC 
GGCCCAATGG GGCCGCTGCC CCCGCAGGCT GGCACTAATC CCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Epn3 ( XM_006534266.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534329.1 Mus musculus epsin 3 (Epn3), transcript variant X1, mRNA.

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CloneID	OMu04899
Clone ID Related Accession (Same CDS sequence)	XM_006534266.2 , NM_027984.3 , XM_006534266.3 , XM_006534266.4
Accession Version	XM_006534266.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	epsin-3 isoform X1
Comment

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
CCAGTCCCCC CAGCCTCCCA CAGGGATGAG GACCTGCAGC TGCAGCTGGC TCTGAGCCTG 
AGCCGGCAAG AGCATGAGAA GGGGGTGAGA TCCTGGAAGG GAGATGACTC TCCAGTGGCC 
AACGGCGCAG AACCTGCTGG CCAACGTCGT CAACGGGACA GGGAGCCTGA GAGAGAAGAG 
AGAAAGGAGG AGGAGAAGCT GAAAACTAGT CAGTCCTCCA TCCTGGACTT GGCTGACATC 
TTCGCACCTG CCCCGGCCCT GCCTTCCACA CACTGCTCTG CTGACCCATG GGACATCCCA 
GGTCTCAGGC CGAACACAGA GCCAAGTGGC TCCTCTTGGG GACCTTCTGC AGACCCCTGG 
TCTCCAGTTC CCTCTGGAAA TGCCCTGTCC CGAAGCCAGC CTTGGGATTT GCTTCCTACT 
CTTTCTTCCT CTGAGCCCTG GGGCAGGACC CCAGTGCTAC CTTCTGGACC CCCCATCGCA 
GACCCCTGGG CACCAAGCTC CCCTACCCGC AAACTCCCCA GCACTGGAGC AGACCCTTGG 
GGGGCCTCTA TGGAGACCTC TGACACCTCT GCTCTAGGTG GTGCCTCGCC CTTCGACCCA 
TTTGCCAAAC CTCTAGAATC CACAGAACCC AAGGAGAGCC GGGACAGTGC CCAGGCCCTG 
CCCACGGGGA AGTCCCCCAG CACTGTGGAG CTAGACCCAT TTGGAGACTC CAGCCCCAGT 
TGCAAGCAGA ATGGCATGAA GGAACCTGAA GCCCTTGACC TGGGTGTGCT AGGAGAGGCG 
CTACCGCAGC AGCCAGGAAA GGAAGCGCGC CCCTGCCGGA CTCCGGAGTC CTTCCTGGGC 
CCCTCTGCCT CTTCTTTGGT CAACTTGGAC TCACTAGTCA AGGCACCCCT GGCTGCAAGG 
ACCCGGAATC CCTTCCTGAC AGGTCTGGGT GTTCCGTCCC CAACCAACCC GTTTGGCGCG 
GGCGATCAGG GCAGGCCGAC CCTAAACCAG ATGCGTACCG GCTCCCCTGC TCTGGGCCTG 
CCGCCGGGGG GACCCGTCGG GGCGCCCGTG GGCTCTATGA CCTACAGCGC CTCTCTGCCC 
CTTCCGCTCA GCAGCGTGCC GGTAGGCGCG ACCCTCCCCG CCTCGGTCAG CGTCTTCCCG 
CAAGCGGGAG CCTTCGCCCC GCCGCCGGCG AGCTTGCCTC AGCCACTGCT GCCCACGTCC 
GGCCCAATGG GGCCGCTGCC CCCGCAGGCT GGCACTAATC CCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006534329.1

Translation

MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPPSSLMSEIADLTFNTVAFAEVMGMVWRRLNDSGKNWRHVYKALTLLDYLLKTGSERVAHQCRENLYTIQTLKDFQYIDRDGKDQGVNVREKVKQVMALLKDEERLRQERTHALKTKERMALEGMGIGSGQLGYSRRSRGSPSSYTSASSSPRYASDLEQARPQTSGEEELQLQLALAMSREEAERPVPPASHRDEDLQLQLALSLSRQEHEKGVRSWKGDDSPVANGAEPAGQRRQRDREPEREERKEEEKLKTSQSSILDLADIFAPAPALPSTHCSADPWDIPGLRPNTEPSGSSWGPSADPWSPVPSGNALSRSQPWDLLPTLSSSEPWGRTPVLPSGPPIADPWAPSSPTRKLPSTGADPWGASMETSDTSALGGASPFDPFAKPLESTEPKESRDSAQALPTGKSPSTVELDPFGDSSPSCKQNGMKEPEALDLGVLGEALPQQPGKEARPCRTPESFLGPSASSLVNLDSLVKAPLAARTRNPFLTGLGVPSPTNPFGAGDQGRPTLNQMRTGSPALGLPPGGPVGAPVGSMTYSASLPLPLSSVPVGATLPASVSVFPQAGAFAPPPASLPQPLLPTSGPMGPLPPQAGTNPFL

Target ORF information:

RefSeq Version	XM_006534266.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus epsin 3 (Epn3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534266.2

ORF Insert Sequence:

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
CCAGTCCCCC CAGCCTCCCA CAGGGATGAG GACCTGCAGC TGCAGCTGGC TCTGAGCCTG 
AGCCGGCAAG AGCATGAGAA GGGGGTGAGA TCCTGGAAGG GAGATGACTC TCCAGTGGCC 
AACGGCGCAG AACCTGCTGG CCAACGTCGT CAACGGGACA GGGAGCCTGA GAGAGAAGAG 
AGAAAGGAGG AGGAGAAGCT GAAAACTAGT CAGTCCTCCA TCCTGGACTT GGCTGACATC 
TTCGCACCTG CCCCGGCCCT GCCTTCCACA CACTGCTCTG CTGACCCATG GGACATCCCA 
GGTCTCAGGC CGAACACAGA GCCAAGTGGC TCCTCTTGGG GACCTTCTGC AGACCCCTGG 
TCTCCAGTTC CCTCTGGAAA TGCCCTGTCC CGAAGCCAGC CTTGGGATTT GCTTCCTACT 
CTTTCTTCCT CTGAGCCCTG GGGCAGGACC CCAGTGCTAC CTTCTGGACC CCCCATCGCA 
GACCCCTGGG CACCAAGCTC CCCTACCCGC AAACTCCCCA GCACTGGAGC AGACCCTTGG 
GGGGCCTCTA TGGAGACCTC TGACACCTCT GCTCTAGGTG GTGCCTCGCC CTTCGACCCA 
TTTGCCAAAC CTCTAGAATC CACAGAACCC AAGGAGAGCC GGGACAGTGC CCAGGCCCTG 
CCCACGGGGA AGTCCCCCAG CACTGTGGAG CTAGACCCAT TTGGAGACTC CAGCCCCAGT 
TGCAAGCAGA ATGGCATGAA GGAACCTGAA GCCCTTGACC TGGGTGTGCT AGGAGAGGCG 
CTACCGCAGC AGCCAGGAAA GGAAGCGCGC CCCTGCCGGA CTCCGGAGTC CTTCCTGGGC 
CCCTCTGCCT CTTCTTTGGT CAACTTGGAC TCACTAGTCA AGGCACCCCT GGCTGCAAGG 
ACCCGGAATC CCTTCCTGAC AGGTCTGGGT GTTCCGTCCC CAACCAACCC GTTTGGCGCG 
GGCGATCAGG GCAGGCCGAC CCTAAACCAG ATGCGTACCG GCTCCCCTGC TCTGGGCCTG 
CCGCCGGGGG GACCCGTCGG GGCGCCCGTG GGCTCTATGA CCTACAGCGC CTCTCTGCCC 
CTTCCGCTCA GCAGCGTGCC GGTAGGCGCG ACCCTCCCCG CCTCGGTCAG CGTCTTCCCG 
CAAGCGGGAG CCTTCGCCCC GCCGCCGGCG AGCTTGCCTC AGCCACTGCT GCCCACGTCC 
GGCCCAATGG GGCCGCTGCC CCCGCAGGCT GGCACTAATC CCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Epn3 ( XM_006534266.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534329.1 Mus musculus epsin 3 (Epn3), transcript variant X1, mRNA.

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CloneID	OMu04899
Clone ID Related Accession (Same CDS sequence)	XM_006534266.2 , NM_027984.3 , XM_006534266.3 , XM_006534266.4
Accession Version	XM_006534266.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	epsin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006534266.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
CCAGTCCCCC CAGCCTCCCA CAGGGATGAG GACCTGCAGC TGCAGCTGGC TCTGAGCCTG 
AGCCGGCAAG AGCATGAGAA GGGGGTGAGA TCCTGGAAGG GAGATGACTC TCCAGTGGCC 
AACGGCGCAG AACCTGCTGG CCAACGTCGT CAACGGGACA GGGAGCCTGA GAGAGAAGAG 
AGAAAGGAGG AGGAGAAGCT GAAAACTAGT CAGTCCTCCA TCCTGGACTT GGCTGACATC 
TTCGCACCTG CCCCGGCCCT GCCTTCCACA CACTGCTCTG CTGACCCATG GGACATCCCA 
GGTCTCAGGC CGAACACAGA GCCAAGTGGC TCCTCTTGGG GACCTTCTGC AGACCCCTGG 
TCTCCAGTTC CCTCTGGAAA TGCCCTGTCC CGAAGCCAGC CTTGGGATTT GCTTCCTACT 
CTTTCTTCCT CTGAGCCCTG GGGCAGGACC CCAGTGCTAC CTTCTGGACC CCCCATCGCA 
GACCCCTGGG CACCAAGCTC CCCTACCCGC AAACTCCCCA GCACTGGAGC AGACCCTTGG 
GGGGCCTCTA TGGAGACCTC TGACACCTCT GCTCTAGGTG GTGCCTCGCC CTTCGACCCA 
TTTGCCAAAC CTCTAGAATC CACAGAACCC AAGGAGAGCC GGGACAGTGC CCAGGCCCTG 
CCCACGGGGA AGTCCCCCAG CACTGTGGAG CTAGACCCAT TTGGAGACTC CAGCCCCAGT 
TGCAAGCAGA ATGGCATGAA GGAACCTGAA GCCCTTGACC TGGGTGTGCT AGGAGAGGCG 
CTACCGCAGC AGCCAGGAAA GGAAGCGCGC CCCTGCCGGA CTCCGGAGTC CTTCCTGGGC 
CCCTCTGCCT CTTCTTTGGT CAACTTGGAC TCACTAGTCA AGGCACCCCT GGCTGCAAGG 
ACCCGGAATC CCTTCCTGAC AGGTCTGGGT GTTCCGTCCC CAACCAACCC GTTTGGCGCG 
GGCGATCAGG GCAGGCCGAC CCTAAACCAG ATGCGTACCG GCTCCCCTGC TCTGGGCCTG 
CCGCCGGGGG GACCCGTCGG GGCGCCCGTG GGCTCTATGA CCTACAGCGC CTCTCTGCCC 
CTTCCGCTCA GCAGCGTGCC GGTAGGCGCG ACCCTCCCCG CCTCGGTCAG CGTCTTCCCG 
CAAGCGGGAG CCTTCGCCCC GCCGCCGGCG AGCTTGCCTC AGCCACTGCT GCCCACGTCC 
GGCCCAATGG GGCCGCTGCC CCCGCAGGCT GGCACTAATC CCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534329.1
CDS	519..2429
Translation	MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPPSSLMSEIADLTFNTVAFAEVMGMVWRRLNDSGKNWRHVYKALTLLDYLLKTGSERVAHQCRENLYTIQTLKDFQYIDRDGKDQGVNVREKVKQVMALLKDEERLRQERTHALKTKERMALEGMGIGSGQLGYSRRSRGSPSSYTSASSSPRYASDLEQARPQTSGEEELQLQLALAMSREEAERPVPPASHRDEDLQLQLALSLSRQEHEKGVRSWKGDDSPVANGAEPAGQRRQRDREPEREERKEEEKLKTSQSSILDLADIFAPAPALPSTHCSADPWDIPGLRPNTEPSGSSWGPSADPWSPVPSGNALSRSQPWDLLPTLSSSEPWGRTPVLPSGPPIADPWAPSSPTRKLPSTGADPWGASMETSDTSALGGASPFDPFAKPLESTEPKESRDSAQALPTGKSPSTVELDPFGDSSPSCKQNGMKEPEALDLGVLGEALPQQPGKEARPCRTPESFLGPSASSLVNLDSLVKAPLAARTRNPFLTGLGVPSPTNPFGAGDQGRPTLNQMRTGSPALGLPPGGPVGAPVGSMTYSASLPLPLSSVPVGATLPASVSVFPQAGAFAPPPASLPQPLLPTSGPMGPLPPQAGTNPFL

Target ORF information:

RefSeq Version	XM_006534266.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus epsin 3 (Epn3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534266.3

ORF Insert Sequence:

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
CCAGTCCCCC CAGCCTCCCA CAGGGATGAG GACCTGCAGC TGCAGCTGGC TCTGAGCCTG 
AGCCGGCAAG AGCATGAGAA GGGGGTGAGA TCCTGGAAGG GAGATGACTC TCCAGTGGCC 
AACGGCGCAG AACCTGCTGG CCAACGTCGT CAACGGGACA GGGAGCCTGA GAGAGAAGAG 
AGAAAGGAGG AGGAGAAGCT GAAAACTAGT CAGTCCTCCA TCCTGGACTT GGCTGACATC 
TTCGCACCTG CCCCGGCCCT GCCTTCCACA CACTGCTCTG CTGACCCATG GGACATCCCA 
GGTCTCAGGC CGAACACAGA GCCAAGTGGC TCCTCTTGGG GACCTTCTGC AGACCCCTGG 
TCTCCAGTTC CCTCTGGAAA TGCCCTGTCC CGAAGCCAGC CTTGGGATTT GCTTCCTACT 
CTTTCTTCCT CTGAGCCCTG GGGCAGGACC CCAGTGCTAC CTTCTGGACC CCCCATCGCA 
GACCCCTGGG CACCAAGCTC CCCTACCCGC AAACTCCCCA GCACTGGAGC AGACCCTTGG 
GGGGCCTCTA TGGAGACCTC TGACACCTCT GCTCTAGGTG GTGCCTCGCC CTTCGACCCA 
TTTGCCAAAC CTCTAGAATC CACAGAACCC AAGGAGAGCC GGGACAGTGC CCAGGCCCTG 
CCCACGGGGA AGTCCCCCAG CACTGTGGAG CTAGACCCAT TTGGAGACTC CAGCCCCAGT 
TGCAAGCAGA ATGGCATGAA GGAACCTGAA GCCCTTGACC TGGGTGTGCT AGGAGAGGCG 
CTACCGCAGC AGCCAGGAAA GGAAGCGCGC CCCTGCCGGA CTCCGGAGTC CTTCCTGGGC 
CCCTCTGCCT CTTCTTTGGT CAACTTGGAC TCACTAGTCA AGGCACCCCT GGCTGCAAGG 
ACCCGGAATC CCTTCCTGAC AGGTCTGGGT GTTCCGTCCC CAACCAACCC GTTTGGCGCG 
GGCGATCAGG GCAGGCCGAC CCTAAACCAG ATGCGTACCG GCTCCCCTGC TCTGGGCCTG 
CCGCCGGGGG GACCCGTCGG GGCGCCCGTG GGCTCTATGA CCTACAGCGC CTCTCTGCCC 
CTTCCGCTCA GCAGCGTGCC GGTAGGCGCG ACCCTCCCCG CCTCGGTCAG CGTCTTCCCG 
CAAGCGGGAG CCTTCGCCCC GCCGCCGGCG AGCTTGCCTC AGCCACTGCT GCCCACGTCC 
GGCCCAATGG GGCCGCTGCC CCCGCAGGCT GGCACTAATC CCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Epn3 ( XM_006534266.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006534329.1 Mus musculus epsin 3 (Epn3), transcript variant X1, mRNA.

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CloneID	OMu04899
Clone ID Related Accession (Same CDS sequence)	XM_006534266.2 , NM_027984.3 , XM_006534266.3 , XM_006534266.4
Accession Version	XM_006534266.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	epsin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006534266.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
CCAGTCCCCC CAGCCTCCCA CAGGGATGAG GACCTGCAGC TGCAGCTGGC TCTGAGCCTG 
AGCCGGCAAG AGCATGAGAA GGGGGTGAGA TCCTGGAAGG GAGATGACTC TCCAGTGGCC 
AACGGCGCAG AACCTGCTGG CCAACGTCGT CAACGGGACA GGGAGCCTGA GAGAGAAGAG 
AGAAAGGAGG AGGAGAAGCT GAAAACTAGT CAGTCCTCCA TCCTGGACTT GGCTGACATC 
TTCGCACCTG CCCCGGCCCT GCCTTCCACA CACTGCTCTG CTGACCCATG GGACATCCCA 
GGTCTCAGGC CGAACACAGA GCCAAGTGGC TCCTCTTGGG GACCTTCTGC AGACCCCTGG 
TCTCCAGTTC CCTCTGGAAA TGCCCTGTCC CGAAGCCAGC CTTGGGATTT GCTTCCTACT 
CTTTCTTCCT CTGAGCCCTG GGGCAGGACC CCAGTGCTAC CTTCTGGACC CCCCATCGCA 
GACCCCTGGG CACCAAGCTC CCCTACCCGC AAACTCCCCA GCACTGGAGC AGACCCTTGG 
GGGGCCTCTA TGGAGACCTC TGACACCTCT GCTCTAGGTG GTGCCTCGCC CTTCGACCCA 
TTTGCCAAAC CTCTAGAATC CACAGAACCC AAGGAGAGCC GGGACAGTGC CCAGGCCCTG 
CCCACGGGGA AGTCCCCCAG CACTGTGGAG CTAGACCCAT TTGGAGACTC CAGCCCCAGT 
TGCAAGCAGA ATGGCATGAA GGAACCTGAA GCCCTTGACC TGGGTGTGCT AGGAGAGGCG 
CTACCGCAGC AGCCAGGAAA GGAAGCGCGC CCCTGCCGGA CTCCGGAGTC CTTCCTGGGC 
CCCTCTGCCT CTTCTTTGGT CAACTTGGAC TCACTAGTCA AGGCACCCCT GGCTGCAAGG 
ACCCGGAATC CCTTCCTGAC AGGTCTGGGT GTTCCGTCCC CAACCAACCC GTTTGGCGCG 
GGCGATCAGG GCAGGCCGAC CCTAAACCAG ATGCGTACCG GCTCCCCTGC TCTGGGCCTG 
CCGCCGGGGG GACCCGTCGG GGCGCCCGTG GGCTCTATGA CCTACAGCGC CTCTCTGCCC 
CTTCCGCTCA GCAGCGTGCC GGTAGGCGCG ACCCTCCCCG CCTCGGTCAG CGTCTTCCCG 
CAAGCGGGAG CCTTCGCCCC GCCGCCGGCG AGCTTGCCTC AGCCACTGCT GCCCACGTCC 
GGCCCAATGG GGCCGCTGCC CCCGCAGGCT GGCACTAATC CCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006534329.1
CDS	229..2139
Translation	MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPPSSLMSEIADLTFNTVAFAEVMGMVWRRLNDSGKNWRHVYKALTLLDYLLKTGSERVAHQCRENLYTIQTLKDFQYIDRDGKDQGVNVREKVKQVMALLKDEERLRQERTHALKTKERMALEGMGIGSGQLGYSRRSRGSPSSYTSASSSPRYASDLEQARPQTSGEEELQLQLALAMSREEAERPVPPASHRDEDLQLQLALSLSRQEHEKGVRSWKGDDSPVANGAEPAGQRRQRDREPEREERKEEEKLKTSQSSILDLADIFAPAPALPSTHCSADPWDIPGLRPNTEPSGSSWGPSADPWSPVPSGNALSRSQPWDLLPTLSSSEPWGRTPVLPSGPPIADPWAPSSPTRKLPSTGADPWGASMETSDTSALGGASPFDPFAKPLESTEPKESRDSAQALPTGKSPSTVELDPFGDSSPSCKQNGMKEPEALDLGVLGEALPQQPGKEARPCRTPESFLGPSASSLVNLDSLVKAPLAARTRNPFLTGLGVPSPTNPFGAGDQGRPTLNQMRTGSPALGLPPGGPVGAPVGSMTYSASLPLPLSSVPVGATLPASVSVFPQAGAFAPPPASLPQPLLPTSGPMGPLPPQAGTNPFL

Target ORF information:

RefSeq Version	XM_006534266.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus epsin 3 (Epn3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006534266.4

ORF Insert Sequence:

ATGACAACCT CTGCACTCCG CCGCCAGGTG AAGAACATTG TTCACAACTA CTCTGAGGCC 
GAGATCAAGG TGCGTGAGGC CACCAGCAAT GACCCCTGGG GCCCACCCAG TTCTCTCATG 
TCAGAGATTG CTGACCTGAC CTTCAACACG GTGGCCTTTG CTGAGGTTAT GGGGATGGTG 
TGGCGGAGGC TCAATGACAG CGGTAAGAAC TGGCGACACG TATACAAGGC TCTGACACTA 
TTGGACTACC TGCTCAAGAC GGGCTCAGAA CGTGTGGCCC ACCAGTGCCG CGAGAACCTC 
TACACTATCC AGACTCTCAA GGACTTCCAG TATATTGACC GAGATGGCAA GGATCAGGGC 
GTCAATGTTC GTGAGAAGGT CAAACAGGTG ATGGCCCTTC TCAAGGATGA GGAGCGCCTG 
CGGCAAGAGC GAACCCATGC TCTCAAGACC AAAGAGCGAA TGGCCCTGGA AGGCATGGGT 
ATTGGCAGCG GGCAGCTGGG CTACAGCCGC AGATCTCGGG GCTCCCCCTC TTCTTACACG 
TCCGCATCTT CGTCCCCGCG CTATGCCTCA GACCTGGAGC AGGCGCGGCC TCAGACTTCT 
GGAGAAGAAG AGCTGCAGCT GCAGCTGGCC TTGGCCATGA GTCGCGAAGA GGCTGAAAGG 
CCAGTCCCCC CAGCCTCCCA CAGGGATGAG GACCTGCAGC TGCAGCTGGC TCTGAGCCTG 
AGCCGGCAAG AGCATGAGAA GGGGGTGAGA TCCTGGAAGG GAGATGACTC TCCAGTGGCC 
AACGGCGCAG AACCTGCTGG CCAACGTCGT CAACGGGACA GGGAGCCTGA GAGAGAAGAG 
AGAAAGGAGG AGGAGAAGCT GAAAACTAGT CAGTCCTCCA TCCTGGACTT GGCTGACATC 
TTCGCACCTG CCCCGGCCCT GCCTTCCACA CACTGCTCTG CTGACCCATG GGACATCCCA 
GGTCTCAGGC CGAACACAGA GCCAAGTGGC TCCTCTTGGG GACCTTCTGC AGACCCCTGG 
TCTCCAGTTC CCTCTGGAAA TGCCCTGTCC CGAAGCCAGC CTTGGGATTT GCTTCCTACT 
CTTTCTTCCT CTGAGCCCTG GGGCAGGACC CCAGTGCTAC CTTCTGGACC CCCCATCGCA 
GACCCCTGGG CACCAAGCTC CCCTACCCGC AAACTCCCCA GCACTGGAGC AGACCCTTGG 
GGGGCCTCTA TGGAGACCTC TGACACCTCT GCTCTAGGTG GTGCCTCGCC CTTCGACCCA 
TTTGCCAAAC CTCTAGAATC CACAGAACCC AAGGAGAGCC GGGACAGTGC CCAGGCCCTG 
CCCACGGGGA AGTCCCCCAG CACTGTGGAG CTAGACCCAT TTGGAGACTC CAGCCCCAGT 
TGCAAGCAGA ATGGCATGAA GGAACCTGAA GCCCTTGACC TGGGTGTGCT AGGAGAGGCG 
CTACCGCAGC AGCCAGGAAA GGAAGCGCGC CCCTGCCGGA CTCCGGAGTC CTTCCTGGGC 
CCCTCTGCCT CTTCTTTGGT CAACTTGGAC TCACTAGTCA AGGCACCCCT GGCTGCAAGG 
ACCCGGAATC CCTTCCTGAC AGGTCTGGGT GTTCCGTCCC CAACCAACCC GTTTGGCGCG 
GGCGATCAGG GCAGGCCGAC CCTAAACCAG ATGCGTACCG GCTCCCCTGC TCTGGGCCTG 
CCGCCGGGGG GACCCGTCGG GGCGCCCGTG GGCTCTATGA CCTACAGCGC CTCTCTGCCC 
CTTCCGCTCA GCAGCGTGCC GGTAGGCGCG ACCCTCCCCG CCTCGGTCAG CGTCTTCCCG 
CAAGCGGGAG CCTTCGCCCC GCCGCCGGCG AGCTTGCCTC AGCCACTGCT GCCCACGTCC 
GGCCCAATGG GGCCGCTGCC CCCGCAGGCT GGCACTAATC CCTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EPSIN 3, A Novel p53 Target, Regulates the Apoptotic Pathway and Gastric Carcinogenesis.
Neoplasia (New York, N.Y.)19(3)185-195(2017 Mar)
Mori J,Tanikawa C,Ohnishi N,Funauchi Y,Toyoshima O,Ueda K,Matsuda K

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits.
eLife3e03311(2014 Aug)
Messa M,Fern?ndez-Busnadiego R,Sun EW,Chen H,Czapla H,Wrasman K,Wu Y,Ko G,Ross T,Wendland B,De Camilli P

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Selective high-level expression of epsin 3 in gastric parietal cells, where it is localized at endocytic sites of apical canaliculi.
Proceedings of the National Academy of Sciences of the United States of America107(50)21511-6(2010 Dec)
Ko G,Paradise S,Chen H,Graham M,Vecchi M,Bianchi F,Cremona O,Di Fiore PP,De Camilli P

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Epn3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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