Isca1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Isca1
Entrez Gene ID	69046
Full Name	iron-sulfur cluster assembly 1
Synonyms	1810010A06Rik,Hbld2
General protein information	Preferred Names iron-sulfur cluster assembly 1 Names iron-sulfur cluster assembly 1 homolog, mitochondrial HESB like domain containing 2 HESB-like domain-containing protein 2 iron-sulfur assembly protein IscA
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_026921.4	NP_081197.1	iron-sulfur cluster assembly 1 homolog, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ISCA1	NP_112202.2
Danio rerio (zebrafish)	isca1	NP_001020349.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002730	XP_312196.1
Canis lupus familiaris (dog)	ISCA1	XP_849435.2
Mus musculus (house mouse)	Isca1	NP_081197.1
Kluyveromyces lactis	KLLA0F18150g	XP_455894.1
Oryza sativa (rice)	Os01g0105800	NP_001172119.1
Oryza sativa (rice)	Os12g0485500	NP_001066782.1
Pan troglodytes (chimpanzee)	ISCA1	NP_001229541.1
Gallus gallus (chicken)	ISCA1	NP_001258865.1
Xenopus tropicalis (tropical clawed frog)	isca1	XP_002935018.1
Drosophila melanogaster (fruit fly)	l(1)G0136	NP_573062.1
Arabidopsis thaliana (thale cress)	AT2G16710	NP_001189536.1
Bos taurus (cattle)	ISCA1	NP_001029642.1
Rattus norvegicus (Norway rat)	Isca1	NP_853657.1
Caenorhabditis elegans (roundworm)	CELE_Y39B6A.3	NP_741696.1

Gene Ontology
Bibliography

Related articles in PubMed

ISCA1 is essential for mitochondrial Fe
Beilschmidt LK, Ollagnier de Choudens S, Fournier M, Sanakis I, Hograindleur MA, Cl?mancey M, Blondin G, Schmucker S, Eisenmann A, Weiss A, Koebel P, Messaddeq N, Puccio H, Martelli A
Nature communications815124(2017 May)

MiRNA-210 modulates a nickel-induced cellular energy metabolism shift by repressing the iron-sulfur cluster assembly proteins ISCU1/2 in Neuro-2a cells.
He M, Lu Y, Xu S, Mao L, Zhang L, Duan W, Liu C, Pi H, Zhang Y, Zhong M, Yu Z, Zhou Z
Cell death & disease5e1090(2014 Feb)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis.
Nilsson R, Schultz IJ, Pierce EL, Soltis KA, Naranuntarat A, Ward DM, Baughman JM, Paradkar PN, Kingsley PD, Culotta VC, Kaplan J, Palis J, Paw BH, Mootha VK
Cell metabolism10(2)119-30(2009 Aug)

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.
Sansom SN, Griffiths DS, Faedo A, Kleinjan DJ, Ruan Y, Smith J, van Heyningen V, Rubenstein JL, Livesey FJ
PLoS genetics5(6)e1000511(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that miR-210 induced repression of ISCU1/2 may contribute to HIF-1alpha-triggered alterations in neuronal energy metabolism after nickel exposure.
Title: MiRNA-210 modulates a nickel-induced cellular energy metabolism shift by repressing the iron-sulfur cluster assembly proteins ISCU1/2 in Neuro-2a cells.

Required for functional heme biosynthesis in erythroid cells.
Title: Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis.

The following Isca1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Isca1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13418	NM_026921.4 Latest version!	Mus musculus iron-sulfur cluster assembly 1 (Isca1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Isca1 ( NM_026921.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081197.1 Mus musculus iron-sulfur cluster assembly 1 (Isca1), mRNA.

Price & Availability↑

CloneID	OMu13418
Clone ID Related Accession (Same CDS sequence)	NM_026921.4
Accession Version	NM_026921.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	iron-sulfur cluster assembly 1 homolog, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC134869.4. On Apr 30, 2007 this sequence version replaced NM_026921.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK090000.1, BC085482.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGTCGGCGT CGTTGGTCCG CGCCACCGTG CGGGCTGTGA GCAAGAGAAA ACTGCAGCCC 
ACGCGGGCGG CCCTCACACT GACCCCCTCT GCTGTAAACA AGATAAAACA ACTTCTTAAA 
GACAAACCTG AGCATGTGGG TCTGAAAGTT GGCGTGCGAA CCAGGGGCTG TAATGGCCTC 
TCTTACAGCC TGGAGTACAC AAAGACAAAA GGAGATTCTG ATGAAGAAGT TATTCAAGAT 
GGAGTCCGAG TGTTCATCGA GAAGAAAGCA CAGCTAACCC TGTTAGGAAC AGAGATGGAC 
TATGTGGAAG ACAAACTGTC CAGTGAGTTT GTGTTCAATA ACCCCAACAT CAAGGGAACC 
TGTGGCTGCG GTGAGAGCTT TCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081197.1
CDS	86..475
Translation	MSASLVRATVRAVSKRKLQPTRAALTLTPSAVNKIKQLLKDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNIKGTCGCGESFHV

Target ORF information:

RefSeq Version	NM_026921.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus iron-sulfur cluster assembly 1 (Isca1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026921.4

ORF Insert Sequence:

ATGTCGGCGT CGTTGGTCCG CGCCACCGTG CGGGCTGTGA GCAAGAGAAA ACTGCAGCCC 
ACGCGGGCGG CCCTCACACT GACCCCCTCT GCTGTAAACA AGATAAAACA ACTTCTTAAA 
GACAAACCTG AGCATGTGGG TCTGAAAGTT GGCGTGCGAA CCAGGGGCTG TAATGGCCTC 
TCTTACAGCC TGGAGTACAC AAAGACAAAA GGAGATTCTG ATGAAGAAGT TATTCAAGAT 
GGAGTCCGAG TGTTCATCGA GAAGAAAGCA CAGCTAACCC TGTTAGGAAC AGAGATGGAC 
TATGTGGAAG ACAAACTGTC CAGTGAGTTT GTGTTCAATA ACCCCAACAT CAAGGGAACC 
TGTGGCTGCG GTGAGAGCTT TCACGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ISCA1 is essential for mitochondrial Fe
Nature communications815124(2017 May)
Beilschmidt LK,Ollagnier de Choudens S,Fournier M,Sanakis I,Hograindleur MA,Cl?mancey M,Blondin G,Schmucker S,Eisenmann A,Weiss A,Koebel P,Messaddeq N,Puccio H,Martelli A

MiRNA-210 modulates a nickel-induced cellular energy metabolism shift by repressing the iron-sulfur cluster assembly proteins ISCU1/2 in Neuro-2a cells.
Cell death & disease5e1090(2014 Feb)
He M,Lu Y,Xu S,Mao L,Zhang L,Duan W,Liu C,Pi H,Zhang Y,Zhong M,Yu Z,Zhou Z

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Discovery of genes essential for heme biosynthesis through large-scale gene expression analysis.
Cell metabolism10(2)119-30(2009 Aug)
Nilsson R,Schultz IJ,Pierce EL,Soltis KA,Naranuntarat A,Ward DM,Baughman JM,Paradkar PN,Kingsley PD,Culotta VC,Kaplan J,Palis J,Paw BH,Mootha VK

The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis.
PLoS genetics5(6)e1000511(2009 Jun)
Sansom SN,Griffiths DS,Faedo A,Kleinjan DJ,Ruan Y,Smith J,van Heyningen V,Rubenstein JL,Livesey FJ

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Isca1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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