ISCA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ISCA1
Entrez Gene ID	81689
Full Name	iron-sulfur cluster assembly 1
Synonyms	HBLD2,ISA1,MMDS5,hIscA
General protein information	Preferred Names iron-sulfur cluster assembly 1 Names iron-sulfur cluster assembly 1 homolog, mitochondrial HESB like domain containing 2 HESB-like domain-containing protein 2 iron-sulfur assembly protein IscA
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q21.33
Summary	ISCA1 is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions (Cozar-Castellano et al., 2004 [PubMed 15262227]).[supplied by OMIM, Mar 2008].
Disorder MIM:	611006
Disorder Html:	Multiple mitochondrial dysfunctions syndrome 5, 617613 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_030940.4	NP_112202.2	iron-sulfur cluster assembly 1 homolog, mitochondrial precursor
NM_030940.3	NP_112202.2	iron-sulfur cluster assembly 1 homolog, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ISCA1	NP_112202.2
Danio rerio (zebrafish)	isca1	NP_001020349.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002730	XP_312196.1
Canis lupus familiaris (dog)	ISCA1	XP_849435.2
Mus musculus (house mouse)	Isca1	NP_081197.1
Kluyveromyces lactis	KLLA0F18150g	XP_455894.1
Oryza sativa (rice)	Os01g0105800	NP_001172119.1
Oryza sativa (rice)	Os12g0485500	NP_001066782.1
Pan troglodytes (chimpanzee)	ISCA1	NP_001229541.1
Gallus gallus (chicken)	ISCA1	NP_001258865.1
Xenopus tropicalis (tropical clawed frog)	isca1	XP_002935018.1
Drosophila melanogaster (fruit fly)	l(1)G0136	NP_573062.1
Arabidopsis thaliana (thale cress)	AT2G16710	NP_001189536.1
Bos taurus (cattle)	ISCA1	NP_001029642.1
Rattus norvegicus (Norway rat)	Isca1	NP_853657.1
Caenorhabditis elegans (roundworm)	CELE_Y39B6A.3	NP_741696.1

Gene Ontology
Bibliography

Related articles in PubMed

A commentary on homozygous p.(Glu87Lys) variant in ISCA1 is associated with a multiple mitochondrial dysfunctions syndrome.
Finsterer J, Zarrouk-Mahjoub S
Journal of human genetics62(9)865-866(2017 Sep)

Homozygous p.(Glu87Lys) variant in ISCA1 is associated with a multiple mitochondrial dysfunctions syndrome.
Shukla A, Hebbar M, Srivastava A, Kadavigere R, Upadhyai P, Kanthi A, Brandau O, Bielas S, Girisha KM
Journal of human genetics62(7)723-727(2017 Jul)

[2Fe-2S] cluster transfer in iron-sulfur protein biogenesis.
Banci L, Brancaccio D, Ciofi-Baffoni S, Del Conte R, Gadepalli R, Mikolajczyk M, Neri S, Piccioli M, Winkelmann J
Proceedings of the National Academy of Sciences of the United States of America111(17)6203-8(2014 Apr)

Frataxin directly stimulates mitochondrial cysteine desulfurase by exposing substrate-binding sites, and a mutant Fe-S cluster scaffold protein with frataxin-bypassing ability acts similarly.
Pandey A, Gordon DM, Pain J, Stemmler TL, Dancis A, Pain D
The Journal of biological chemistry288(52)36773-86(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The structural plasticity of the dimeric state of the [2Fe-2S] bound form of human GRX5 is the crucial factor that allows an efficient cluster transfer to the partner proteins human ISCA1 and ISCA2 by a specific protein-protein recognition mechanism.
Title: [2Fe-2S] cluster transfer in iron-sulfur protein biogenesis.

The data presented here show that the Isu1 suppressor mimics the frataxin effects on Nfs1, explaining the bypassing activity.
Title: Frataxin directly stimulates mitochondrial cysteine desulfurase by exposing substrate-binding sites, and a mutant Fe-S cluster scaffold protein with frataxin-bypassing ability acts similarly.

ISCA1, ISCA2, and IBA57 were depleted by RNA interference. Depleted cells contained massively swollen and enlarged mitochondria that were virtually devoid of cristae membranes, demonstrating the importance of these proteins for mitochondrial biogenesis.
Title: The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are required for [4Fe-4S] protein maturation.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Iron-binding activity of human iron-sulfur cluster assembly protein hIscA1
Title: Iron-binding activity of human iron-sulfur cluster assembly protein hIscA1.

The following ISCA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ISCA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17122	NM_030940.4 Latest version!	Homo sapiens iron-sulfur cluster assembly 1 (ISCA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu17122	NM_030940.3	Homo sapiens iron-sulfur cluster assembly 1 (ISCA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ISCA1 ( NM_030940.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_112202.2 Homo sapiens iron-sulfur cluster assembly 1 (ISCA1), mRNA.

Price & Availability↑

CloneID	OHu17122
Clone ID Related Accession (Same CDS sequence)	NM_030940.4 , NM_030940.3
Accession Version	NM_030940.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	iron-sulfur cluster assembly 1 homolog, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG772866.1, BC002675.2, BC071621.1, AI436792.1 and AA745613.1. On Nov 22, 2018 this sequence version replaced NM_030940.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225937.1, SRR3476690.915749.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820691 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000375991.9/ ENSP00000365159.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGCTT CCTTAGTCCG GGCAACTGTC CGGGCTGTGA GCAAGAGGAA GCTGCAGCCC 
ACCCGGGCAG CCCTCACCCT GACACCTTCA GCAGTAAACA AGATAAAACA ACTTCTTAAA 
GATAAGCCTG AGCATGTAGG TGTAAAAGTT GGTGTCCGAA CCAGGGGCTG TAATGGCCTT 
TCTTATACTC TAGAATATAC AAAGACAAAA GGAGATTCTG ATGAAGAAGT TATTCAAGAT 
GGAGTCAGAG TATTCATCGA AAAGAAAGCA CAGCTAACAC TTTTAGGAAC AGAAATGGAC 
TATGTTGAAG ACAAATTATC CAGTGAGTTT GTGTTCAATA ACCCAAACAT CAAAGGGACT 
TGTGGCTGTG GAGAAAGCTT TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112202.2
CDS	81..470
Translation	MSASLVRATVRAVSKRKLQPTRAALTLTPSAVNKIKQLLKDKPEHVGVKVGVRTRGCNGLSYTLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNIKGTCGCGESFNI

Target ORF information:

RefSeq Version	NM_030940.4
Organism	Homo sapiens(human)
Definition	Homo sapiens iron-sulfur cluster assembly 1 (ISCA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030940.4

ORF Insert Sequence:

ATGTCGGCTT CCTTAGTCCG GGCAACTGTC CGGGCTGTGA GCAAGAGGAA GCTGCAGCCC 
ACCCGGGCAG CCCTCACCCT GACACCTTCA GCAGTAAACA AGATAAAACA ACTTCTTAAA 
GATAAGCCTG AGCATGTAGG TGTAAAAGTT GGTGTCCGAA CCAGGGGCTG TAATGGCCTT 
TCTTATACTC TAGAATATAC AAAGACAAAA GGAGATTCTG ATGAAGAAGT TATTCAAGAT 
GGAGTCAGAG TATTCATCGA AAAGAAAGCA CAGCTAACAC TTTTAGGAAC AGAAATGGAC 
TATGTTGAAG ACAAATTATC CAGTGAGTTT GTGTTCAATA ACCCAAACAT CAAAGGGACT 
TGTGGCTGTG GAGAAAGCTT TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ISCA1 ( NM_030940.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_112202.2 Homo sapiens iron-sulfur cluster assembly 1 (ISCA1), mRNA.

Price & Availability↑

CloneID	OHu17122
Clone ID Related Accession (Same CDS sequence)	NM_030940.4 , NM_030940.3
Accession Version	NM_030940.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	iron-sulfur cluster assembly 1 homolog, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG772866.1, BC002675.2, BC071621.1, AI436792.1 and AA745613.1. On Sep 22, 2004 this sequence version replaced NM_030940.2. ##Evidence-Data-START## Transcript exon combination :: AK225937.1, SRR1803614.35100.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGGCTT CCTTAGTCCG GGCAACTGTC CGGGCTGTGA GCAAGAGGAA GCTGCAGCCC 
ACCCGGGCAG CCCTCACCCT GACACCTTCA GCAGTAAACA AGATAAAACA ACTTCTTAAA 
GATAAGCCTG AGCATGTAGG TGTAAAAGTT GGTGTCCGAA CCAGGGGCTG TAATGGCCTT 
TCTTATACTC TAGAATATAC AAAGACAAAA GGAGATTCTG ATGAAGAAGT TATTCAAGAT 
GGAGTCAGAG TATTCATCGA AAAGAAAGCA CAGCTAACAC TTTTAGGAAC AGAAATGGAC 
TATGTTGAAG ACAAATTATC CAGTGAGTTT GTGTTCAATA ACCCAAACAT CAAAGGGACT 
TGTGGCTGTG GAGAAAGCTT TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112202.2
CDS	118..507
Translation	MSASLVRATVRAVSKRKLQPTRAALTLTPSAVNKIKQLLKDKPEHVGVKVGVRTRGCNGLSYTLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNIKGTCGCGESFNI

Target ORF information:

RefSeq Version	NM_030940.3
Organism	Homo sapiens(human)
Definition	Homo sapiens iron-sulfur cluster assembly 1 (ISCA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030940.3

ORF Insert Sequence:

ATGTCGGCTT CCTTAGTCCG GGCAACTGTC CGGGCTGTGA GCAAGAGGAA GCTGCAGCCC 
ACCCGGGCAG CCCTCACCCT GACACCTTCA GCAGTAAACA AGATAAAACA ACTTCTTAAA 
GATAAGCCTG AGCATGTAGG TGTAAAAGTT GGTGTCCGAA CCAGGGGCTG TAATGGCCTT 
TCTTATACTC TAGAATATAC AAAGACAAAA GGAGATTCTG ATGAAGAAGT TATTCAAGAT 
GGAGTCAGAG TATTCATCGA AAAGAAAGCA CAGCTAACAC TTTTAGGAAC AGAAATGGAC 
TATGTTGAAG ACAAATTATC CAGTGAGTTT GTGTTCAATA ACCCAAACAT CAAAGGGACT 
TGTGGCTGTG GAGAAAGCTT TAATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A commentary on homozygous p.(Glu87Lys) variant in ISCA1 is associated with a multiple mitochondrial dysfunctions syndrome.
Journal of human genetics62(9)865-866(2017 Sep)
Finsterer J,Zarrouk-Mahjoub S

Homozygous p.(Glu87Lys) variant in ISCA1 is associated with a multiple mitochondrial dysfunctions syndrome.
Journal of human genetics62(7)723-727(2017 Jul)
Shukla A,Hebbar M,Srivastava A,Kadavigere R,Upadhyai P,Kanthi A,Brandau O,Bielas S,Girisha KM

[2Fe-2S] cluster transfer in iron-sulfur protein biogenesis.
Proceedings of the National Academy of Sciences of the United States of America111(17)6203-8(2014 Apr)
Banci L,Brancaccio D,Ciofi-Baffoni S,Del Conte R,Gadepalli R,Mikolajczyk M,Neri S,Piccioli M,Winkelmann J

Frataxin directly stimulates mitochondrial cysteine desulfurase by exposing substrate-binding sites, and a mutant Fe-S cluster scaffold protein with frataxin-bypassing ability acts similarly.
The Journal of biological chemistry288(52)36773-86(2013 Dec)
Pandey A,Gordon DM,Pain J,Stemmler TL,Dancis A,Pain D

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ISCA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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