Rnaseh2b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnaseh2b
Entrez Gene ID	67153
Full Name	ribonuclease H2, subunit B
Synonyms	1110019N06Rik,2610207P08Rik,Dleu8
General protein information	Preferred Names ribonuclease H2, subunit B Names ribonuclease H2 subunit B RNase H2 subunit B deleted in lymphocytic leukemia 8 homolog ribonuclease HI subunit B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_026001.3	NP_080277.1	ribonuclease H2 subunit B
NM_026001.2	NP_080277.1	ribonuclease H2 subunit B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RNASEH2B	XP_001158221.1
	LOC707995	XP_001105134.1
Rattus norvegicus (Norway rat)	Rnaseh2b	NP_001007008.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008161	XP_001237910.2
Bos taurus (cattle)	RNASEH2B	NP_001160038.1
Gallus gallus (chicken)	RNASEH2B	NP_001025997.1
Drosophila melanogaster (fruit fly)	CG11164	NP_572908.2
Mus musculus (house mouse)	Rnaseh2b	NP_080277.1
Xenopus tropicalis (tropical clawed frog)	rnaseh2b	NP_001016329.1
Homo sapiens (human)	RNASEH2B	NP_078846.2
Canis lupus familiaris (dog)	LOC607281	XP_005634133.1
Danio rerio (zebrafish)	rnaseh2b	NP_956565.1

Gene Ontology
Bibliography

Related articles in PubMed

Absence of RNase H2 triggers generation of immunogenic micronuclei removed by autophagy.
Bartsch K, Knittler K, Borowski C, Rudnik S, Damme M, Aden K, Spehlmann ME, Frey N, Saftig P, Chalaris A, Rabe B
Human molecular genetics26(20)3960-3972(2017 Oct)

cGAS surveillance of micronuclei links genome instability to innate immunity.
Mackenzie KJ, Carroll P, Martin CA, Murina O, Fluteau A, Simpson DJ, Olova N, Sutcliffe H, Rainger JK, Leitch A, Osborn RT, Wheeler AP, Nowotny M, Gilbert N, Chandra T, Reijns MAM, Jackson AP
Nature548(7668)461-465(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ribonuclease H2 mutations induce a cGAS/STING-dependent innate immune response
Title: Ribonuclease H2 mutations induce a cGAS/STING-dependent innate immune response.

Recent evidence gathered from ribonuclease H2 knock-out mice provides insight into the molecular mechanisms underlying Aicardi-Goutieres syndrome development and a potential role of DNA damage as a trigger of autoimmunity is discussed. [review]
Title: Aicardi-Gouti?res syndrome: clues from the RNase H2 knock-out mouse.

Study performed targeted mutagenesis of the mouse Rnaseh2b gene to gain insight into the in vivo role of the mammalian RNase H2 enzyme. Ablation of Rnaseh2b in mice leads to early embryonic lethality due to elevated DNA damage and reduced cellular proliferation during gastrulation.
Title: Enzymatic removal of ribonucleotides from DNA is essential for mammalian genome integrity and development.

Data show that the interwoven architecture of RNase H2B and RNase H2C that interface with RNase H2A in a complex ideally suited for nucleic acid binding and hydrolysis coupled to protein-protein interaction motifs.
Title: The structure of the mammalian RNase H2 complex provides insight into RNA.NA hybrid processing to prevent immune dysfunction.

The following Rnaseh2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnaseh2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01387	NM_026001.3 Latest version!	Mus musculus ribonuclease H2, subunit B (Rnaseh2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OMu01387	NM_026001.2	Mus musculus ribonuclease H2, subunit B (Rnaseh2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnaseh2b ( NM_026001.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080277.1 Mus musculus ribonuclease H2, subunit B (Rnaseh2b), mRNA.

Price & Availability↑

CloneID	OMu01387
Clone ID Related Accession (Same CDS sequence)	NM_026001.3 , NM_026001.2
Accession Version	NM_026001.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	ribonuclease H2 subunit B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK011914.1, AK082752.1 and AC154805.2. On Apr 17, 2018 this sequence version replaced NM_026001.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK082752.1, AK011914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGAG GTCGGGACCG CGGGGACTTG GCGGCCAGGC AGCTAGTGTT CCTACTTCCA 
GAACATTTAA AAGATGCCTC GAAGAAGAAG AAGAAGAGCA GCCTCCTGTT CGTAAAGCTG 
GCCAACCCGC ACTCAGGGGA AGGAGCCACA TACTTAATTG ATATGTGTCT TCAACAGCTG 
TTTGAAATAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA 
TCAGGGGGCC TTCTCCACTT TGCCACACCC ATGGATCCAT TGTTCCTGCT CCTTCACTAT 
CTCCTAAAGG CTGGCAAAGA GGGGAAGTAT CAGCCCTTGG ACCAAGTCGT GGTCGATGAC 
ACGTTTCCAG ATTGCACCTT GCTGCTGAGA TTTCCTGAGC TTGAAAAGTC ACTTCGGCAT 
GTGACAGAGG AAAAAGAAGT GAACAGCAAG AAGTACTATA AGTACAGCTC AGAGAAGACA 
TTGAAGTGGC TGGAGAAGAA GGTCAACCAA ACTGTGGTGG CACTAAAAGC TAATAATGTC 
AATGTTGGAG CCCGGGTTCA GTCATCTGCA TATTTCTCTG GTGGTCAGGT TTCCAGGGAC 
AAGGAAGAGG ATTATGTTCG CTATGCCCAT GGTCTGATCT CTGATTACAT CCCTAAAGAA 
CTGAGTGATG ATTTATCCAA GTTCTTGAAG CTTCCAGAAC CTCCAGCTTC ATTGACCAAC 
CCTCCATCAA AGAAACTAAA GTTATCAGAT GAGCCTGTAG AAGCCAAAGA AGATTACACT 
AAGTTTAACA CTAAAGACTT GAAGACCGGC AAGAAAAATA GCAAAATGAC TGCAGCTCAG 
AAGGCTTTGG CTAAAGTTGA CAAAAGTGGA ATGAAAAGTA TCGATGCCTT TTTTGGTGCA 
AAAAATAAAA AAACTGGAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080277.1
CDS	49..975
Translation	MAGGRDRGDLAARQLVFLLPEHLKDASKKKKKSSLLFVKLANPHSGEGATYLIDMCLQQLFEIKVFKEKHHSWFINQSVQSGGLLHFATPMDPLFLLLHYLLKAGKEGKYQPLDQVVVDDTFPDCTLLLRFPELEKSLRHVTEEKEVNSKKYYKYSSEKTLKWLEKKVNQTVVALKANNVNVGARVQSSAYFSGGQVSRDKEEDYVRYAHGLISDYIPKELSDDLSKFLKLPEPPASLTNPPSKKLKLSDEPVEAKEDYTKFNTKDLKTGKKNSKMTAAQKALAKVDKSGMKSIDAFFGAKNKKTGKI

Target ORF information:

RefSeq Version	NM_026001.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribonuclease H2, subunit B (Rnaseh2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026001.3

ORF Insert Sequence:

ATGGCCGGAG GTCGGGACCG CGGGGACTTG GCGGCCAGGC AGCTAGTGTT CCTACTTCCA 
GAACATTTAA AAGATGCCTC GAAGAAGAAG AAGAAGAGCA GCCTCCTGTT CGTAAAGCTG 
GCCAACCCGC ACTCAGGGGA AGGAGCCACA TACTTAATTG ATATGTGTCT TCAACAGCTG 
TTTGAAATAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA 
TCAGGGGGCC TTCTCCACTT TGCCACACCC ATGGATCCAT TGTTCCTGCT CCTTCACTAT 
CTCCTAAAGG CTGGCAAAGA GGGGAAGTAT CAGCCCTTGG ACCAAGTCGT GGTCGATGAC 
ACGTTTCCAG ATTGCACCTT GCTGCTGAGA TTTCCTGAGC TTGAAAAGTC ACTTCGGCAT 
GTGACAGAGG AAAAAGAAGT GAACAGCAAG AAGTACTATA AGTACAGCTC AGAGAAGACA 
TTGAAGTGGC TGGAGAAGAA GGTCAACCAA ACTGTGGTGG CACTAAAAGC TAATAATGTC 
AATGTTGGAG CCCGGGTTCA GTCATCTGCA TATTTCTCTG GTGGTCAGGT TTCCAGGGAC 
AAGGAAGAGG ATTATGTTCG CTATGCCCAT GGTCTGATCT CTGATTACAT CCCTAAAGAA 
CTGAGTGATG ATTTATCCAA GTTCTTGAAG CTTCCAGAAC CTCCAGCTTC ATTGACCAAC 
CCTCCATCAA AGAAACTAAA GTTATCAGAT GAGCCTGTAG AAGCCAAAGA AGATTACACT 
AAGTTTAACA CTAAAGACTT GAAGACCGGC AAGAAAAATA GCAAAATGAC TGCAGCTCAG 
AAGGCTTTGG CTAAAGTTGA CAAAAGTGGA ATGAAAAGTA TCGATGCCTT TTTTGGTGCA 
AAAAATAAAA AAACTGGAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rnaseh2b ( NM_026001.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080277.1 Mus musculus ribonuclease H2, subunit B (Rnaseh2b), mRNA.

Price & Availability↑

CloneID	OMu01387
Clone ID Related Accession (Same CDS sequence)	NM_026001.3 , NM_026001.2
Accession Version	NM_026001.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-13
Organism	Mus musculus(house mouse)
Product	ribonuclease H2 subunit B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK082752.1. On Apr 6, 2007 this sequence version replaced NM_026001.1. ##Evidence-Data-START## Transcript exon combination :: AK082752.1, AK011914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGGAG GTCGGGACCG CGGGGACTTG GCGGCCAGGC AGCTAGTGTT CCTACTTCCA 
GAACATTTAA AAGATGCCTC GAAGAAGAAG AAGAAGAGCA GCCTCCTGTT CGTAAAGCTG 
GCCAACCCGC ACTCAGGGGA AGGAGCCACA TACTTAATTG ATATGTGTCT TCAACAGCTG 
TTTGAAATAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA 
TCAGGGGGCC TTCTCCACTT TGCCACACCC ATGGATCCAT TGTTCCTGCT CCTTCACTAT 
CTCCTAAAGG CTGGCAAAGA GGGGAAGTAT CAGCCCTTGG ACCAAGTCGT GGTCGATGAC 
ACGTTTCCAG ATTGCACCTT GCTGCTGAGA TTTCCTGAGC TTGAAAAGTC ACTTCGGCAT 
GTGACAGAGG AAAAAGAAGT GAACAGCAAG AAGTACTATA AGTACAGCTC AGAGAAGACA 
TTGAAGTGGC TGGAGAAGAA GGTCAACCAA ACTGTGGTGG CACTAAAAGC TAATAATGTC 
AATGTTGGAG CCCGGGTTCA GTCATCTGCA TATTTCTCTG GTGGTCAGGT TTCCAGGGAC 
AAGGAAGAGG ATTATGTTCG CTATGCCCAT GGTCTGATCT CTGATTACAT CCCTAAAGAA 
CTGAGTGATG ATTTATCCAA GTTCTTGAAG CTTCCAGAAC CTCCAGCTTC ATTGACCAAC 
CCTCCATCAA AGAAACTAAA GTTATCAGAT GAGCCTGTAG AAGCCAAAGA AGATTACACT 
AAGTTTAACA CTAAAGACTT GAAGACCGGC AAGAAAAATA GCAAAATGAC TGCAGCTCAG 
AAGGCTTTGG CTAAAGTTGA CAAAAGTGGA ATGAAAAGTA TCGATGCCTT TTTTGGTGCA 
AAAAATAAAA AAACTGGAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080277.1
CDS	12..938
Translation	MAGGRDRGDLAARQLVFLLPEHLKDASKKKKKSSLLFVKLANPHSGEGATYLIDMCLQQLFEIKVFKEKHHSWFINQSVQSGGLLHFATPMDPLFLLLHYLLKAGKEGKYQPLDQVVVDDTFPDCTLLLRFPELEKSLRHVTEEKEVNSKKYYKYSSEKTLKWLEKKVNQTVVALKANNVNVGARVQSSAYFSGGQVSRDKEEDYVRYAHGLISDYIPKELSDDLSKFLKLPEPPASLTNPPSKKLKLSDEPVEAKEDYTKFNTKDLKTGKKNSKMTAAQKALAKVDKSGMKSIDAFFGAKNKKTGKI

Target ORF information:

RefSeq Version	NM_026001.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ribonuclease H2, subunit B (Rnaseh2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026001.2

ORF Insert Sequence:

ATGGCCGGAG GTCGGGACCG CGGGGACTTG GCGGCCAGGC AGCTAGTGTT CCTACTTCCA 
GAACATTTAA AAGATGCCTC GAAGAAGAAG AAGAAGAGCA GCCTCCTGTT CGTAAAGCTG 
GCCAACCCGC ACTCAGGGGA AGGAGCCACA TACTTAATTG ATATGTGTCT TCAACAGCTG 
TTTGAAATAA AAGTTTTCAA GGAAAAACAC CATTCTTGGT TTATAAATCA ATCAGTTCAA 
TCAGGGGGCC TTCTCCACTT TGCCACACCC ATGGATCCAT TGTTCCTGCT CCTTCACTAT 
CTCCTAAAGG CTGGCAAAGA GGGGAAGTAT CAGCCCTTGG ACCAAGTCGT GGTCGATGAC 
ACGTTTCCAG ATTGCACCTT GCTGCTGAGA TTTCCTGAGC TTGAAAAGTC ACTTCGGCAT 
GTGACAGAGG AAAAAGAAGT GAACAGCAAG AAGTACTATA AGTACAGCTC AGAGAAGACA 
TTGAAGTGGC TGGAGAAGAA GGTCAACCAA ACTGTGGTGG CACTAAAAGC TAATAATGTC 
AATGTTGGAG CCCGGGTTCA GTCATCTGCA TATTTCTCTG GTGGTCAGGT TTCCAGGGAC 
AAGGAAGAGG ATTATGTTCG CTATGCCCAT GGTCTGATCT CTGATTACAT CCCTAAAGAA 
CTGAGTGATG ATTTATCCAA GTTCTTGAAG CTTCCAGAAC CTCCAGCTTC ATTGACCAAC 
CCTCCATCAA AGAAACTAAA GTTATCAGAT GAGCCTGTAG AAGCCAAAGA AGATTACACT 
AAGTTTAACA CTAAAGACTT GAAGACCGGC AAGAAAAATA GCAAAATGAC TGCAGCTCAG 
AAGGCTTTGG CTAAAGTTGA CAAAAGTGGA ATGAAAAGTA TCGATGCCTT TTTTGGTGCA 
AAAAATAAAA AAACTGGAAA GATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Absence of RNase H2 triggers generation of immunogenic micronuclei removed by autophagy.
Human molecular genetics26(20)3960-3972(2017 Oct)
Bartsch K,Knittler K,Borowski C,Rudnik S,Damme M,Aden K,Spehlmann ME,Frey N,Saftig P,Chalaris A,Rabe B

cGAS surveillance of micronuclei links genome instability to innate immunity.
Nature548(7668)461-465(2017 08)
Mackenzie KJ,Carroll P,Martin CA,Murina O,Fluteau A,Simpson DJ,Olova N,Sutcliffe H,Rainger JK,Leitch A,Osborn RT,Wheeler AP,Nowotny M,Gilbert N,Chandra T,Reijns MAM,Jackson AP

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Rnaseh2b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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