Myo3a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myo3a
Entrez Gene ID	667663
Full Name	myosin IIIA
Synonyms	9030416P08Rik
General protein information	Preferred Names myosin IIIA Names myosin-IIIa
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_148413.3	NP_680779.3	myosin-IIIa

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYO3A	XP_507703.2
Mus musculus (house mouse)	Myo3a	NP_680779.3
	MYO3A	XP_001101244.2
Xenopus tropicalis (tropical clawed frog)	myo3a	XP_002933189.2
Homo sapiens (human)	MYO3A	NP_059129.3
Rattus norvegicus (Norway rat)	Myo3a	XP_574090.4
Gallus gallus (chicken)	MYO3A	XP_418597.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009730	XP_318814.4
Canis lupus familiaris (dog)	MYO3A	XP_005617041.1
Drosophila melanogaster (fruit fly)	ninaC	NP_523503.2
Bos taurus (cattle)	MYO3A	XP_002692119.1
Danio rerio (zebrafish)	myo3a	NP_991142.1

Gene Ontology
Bibliography

Related articles in PubMed

Stereocilia-staircase spacing is influenced by myosin III motors and their cargos espin-1 and espin-like.
Ebrahim S, Avenarius MR, Grati M, Krey JF, Windsor AM, Sousa AD, Ballesteros A, Cui R, Millis BA, Salles FT, Baird MA, Davidson MW, Jones SM, Choi D, Dong L, Raval MH, Yengo CM, Barr-Gillespie PG, Kachar B
Nature communications710833(2016 Mar)

Class III myosins shape the auditory hair bundles by limiting microvilli and stereocilia growth.
Lelli A, Michel V, Boutet de Monvel J, Cortese M, Bosch-Grau M, Aghaie A, Perfettini I, Dupont T, Avan P, El-Amraoui A, Petit C
The Journal of cell biology212(2)231-44(2016 Jan)

Myosin IIIB uses an actin-binding motif in its espin-1 cargo to reach the tips of actin protrusions.
Merritt RC, Manor U, Salles FT, Grati M, Dose AC, Unrath WC, Quintero OA, Yengo CM, Kachar B
Current biology : CB22(4)320-5(2012 Feb)

Mouse class III myosins: kinase activity and phosphorylation sites.
Dalal JS, Stevens SM Jr, Alvarez S, Munoz N, Kempler KE, Dos? AC, Burnside B, Battelle BA
Journal of neurochemistry119(4)772-84(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results uncover the critical role that class III myosins play redundantly in hair-bundle morphogenesis
Title: Class III myosins shape the auditory hair bundles by limiting microvilli and stereocilia growth.

The phenotype of the Myo3a knockout mice parallels the phenotype of human DFNB30.
Title: A mouse model for human hearing loss DFNB30 due to loss of function of myosin IIIA.

The following Myo3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myo3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02112	NM_148413.3 Latest version!	Mus musculus myosin IIIA (Myo3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$958.30 $1369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myo3a ( NM_148413.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_680779.3 Mus musculus myosin IIIA (Myo3a), mRNA.

Price & Availability↑

CloneID	OMu02112
Clone ID Related Accession (Same CDS sequence)	NM_148413.3
Accession Version	NM_148413.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	myosin-IIIa
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL929064.11, AL929036.8, CAAA01176773.1, CAAA01085045.1, CAAA01172990.1 and AL928693.9. On Mar 19, 2010 this sequence version replaced NM_148413.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849377, SAMN00849384 [ECO:0000350] ##Evidence-Data-END##

ATGCAGATGA CAGAAACCTT TGAGATGCTT CCATTAATTG GAAAAACAAT CATCTTTGAT 
AACTTTCCTG ATCCTTCTGA TACATGGGAA ATCATTGAAA CCATCGGCAA AGGAACTTAT 
GGGAAGGTTT TCAAAGTCCT GAATAAGAAA AGTGGCCAGA AAGCAGCAGT CAAAATTTTG 
GATCCAATCC ATGATATCGA TGAAGAGATT GAAGCAGAGT ATAACATCTT AAGAACACTT 
TCTGACCACC CAAATGTGGT CAGATTCTAC GGGATTTACT TTAAAAAGGA CAAGATAAAT 
GGAGATAAGC TATGGCTGGT TCTGGAGCTG TGCAATGGAG GATCAGTGAC TGATCTTGTG 
AAAGGATTTC TGAAGAGGGG AGAAAGAATG AGCGAGCCTG TAATTGCATA TATTTTACAC 
GAAGCACTAA TGGGACTTCA GCATCTTCAT AGCAACAAAA CCATCCACCG TGATGTCAAA 
GGAAATAACA TCCTGTTGAC TACTGAAGGT GGAGTAAAAC TAGTAGATTT TGGTGTGTCT 
GCACAGCTCA GCAGCACCCG GCACCGCCTG AACACCTCCG TAGGAACACC ATTTTGGATG 
GCTCCTGAGG TGATTGCTTG TGAACAGCAA CTAGATACCA CTTACGATGC TAGATGTGAC 
ACATGGTCCC TGGGTATCAC GGCTATTGAG CTTGGTGATG GAGATCCTCC ACTAGCTGAG 
CTGCATCCCA TGAGAGCACT CTTCAAAATA CCAAGGAATC CACCCCCTAA ACTCCGGCAG 
CCTGAGCTGT GGTCCGCAGA GTTCAATGAC TTCATTAGCA AATGCTTGAC CAAAGATTAT 
GAAAAGCGTC CAACAGTGTC AGATCTTTTG AAGCATAAAT TTATTACTCA GATCGAGGGC 
AAAGATGTGA TACTACAAAA ACAACTAATG GAATTCATTG ATATCCATCA ATGCCTGGGA 
AGCACAGAGA AGGCCAGGCA TGAACGAATT CATACCAAGA AAGGTAATTT AAATAGATCT 
CTCATTTCCA GTCTGAAGGA TGTAGACGAT TTGGCAACCC TAGATGTTCT GGATGAGAAC 
ACAGTGTCTG AACATCTTGA GAAATGTTAT TCCAGAGATC AGATCTATAT TTATGTGGGA 
GATATTCTCA TTGCACTGAA CCCTTTTCAG AGTCTGGGTA TTTATTCCAC AAAGCTTTCT 
AGGTTGTATA TTGGAGCAAA GAGAACTGCC AACCCACCTC ACATTTTTGC AATGGCTGAT 
TTGGGATACC AGTCTATGGT CACATATAAT GCAGACCAGT GCATCGTTAT TTCTGGAGAA 
AGTGGTGCTG GCAAGACAGA GAGCGCTCAT CTTCTGGTTC AACAGCTCAC TGTGCTTGGA 
AAGGCTAATA ACAGAACCCT GCAAGAGAAG ATTTTACAAA TGAACAATTT GGTTGAAGCC 
TTTGGCAATG CCTGCACTAT TATAAATGAC AACTCCAGCA GATTTGGAAA ATACTTAGAA 
ATGAAATTTA CTTCTTCTGG AGCTGTAGTT GGGGCACAGA TTTCTGAATA CCTTCTGGAA 
AAATCCCGTG TCATTCACCA AGCCATTGGA GAAAAAAATT TTCATATTTT TTACTACATT 
TATGCTGGGT TAGCAGAAAA GAAGAAACTA GCTCTTTACA AATTGCCTGA AAACAAGCCA 
CCCAGGTACT TACAAAATGA CAACCTCAGA ACAGTACAGG ACATGATGAA CAATAGTTTC 
TATAAATCCC AGTATGAATT AATTGAACAA TGTTTCAAAG TCATAGGTTT TACAATGGAG 
CAACTTGGAA GTGTATATAG TGTACTCGCC GCCATCTTGA ACGTTGGAAA CATTGAATTC 
TCCTCGGTGG CAACTGAATA CCAGATGGAC AAGAGCTACA TTTGCAATCA CACTGCTCTG 
GAAAACTCCG CTTCTTTGCT TTGCATTCAA GCAGATGAGC TGCAGGAAGC TCTCACCTCA 
CACTGTGTGG TCACTAGAGG AGAAACAATT ATTCGACCCA ACACAGTGGA AAAGGCTGCT 
GATGTCCGAG ATGCCATGGC CAAAACTTTA TATGGCCGTC TCTTTAGCTG GATAGTCAAT 
TGTATTAACA GCTTGCTGAA GCATGACACA TCACCTAGTG GGGATGAGGA GCTAAACATC 
GGCATTCTTG ACATATTCGG CTTTGAAAAC TTCAAAAGAA ATTCCTTTGA GCAGCTGTGC 
ATTAACATTG CAAATGAACA GATCCAGTAT TATTTTAATC AGCACGTGTT TGCATGGGAG 
CAGAATGAAT ACCTAAATGA AGATGTAGAT GCTAGAGTTA TTGAATACGA AGACAACCGG 
CCCCTCTTGG ACATGTTTCT ACAGAAGCCC ATGGGCTTAT TGTCTCTGCT TGATGAGGAA 
AGCAGATTTC CCAAGGCCAC TGACCAGACT CTCATAGAAA AATTTGAAGA CAACCTGAAG 
TCACAGTACT TCTGGAGACC CAAAAGAATG GAACTCAGTT TTGGCATTCA CCACTATGCA 
GGAAAAGTCC TCTATAGTGC TAGTGGGTTC TTGGCTAAAA ACAGAGACAC TTTCCCAACT 
GATATTGTGC TGCTTTTGAG GTCATCAGAA AACAGTGTAA TTCGGCAACT TGTCAACCAC 
CCTCTGACCA AAACAGGTAA TCTGCCACTT TCTAAAACTA AAAACATAGT AAACTATCAG 
ATGTGGAATT CAGAGAAATC CACAAACCTG ACCAAGGGTG AAACTAGAGA TGTCACATGC 
CATGCCTGTG AAACCACCAA TGTGAAAACA CAAACGGTCT CATCCTATTT TAGATACTCC 
TTGATGGATT TATTATCTAA AATGGTAGTG GGCCAACCTC ATTTTGTCCG TTGCATCAAA 
CCAAATAATG AGCGACAGGC AAGAAAGTAT GACAAGGAGA AAGTTCTGCT CCAGCTGCGT 
TGCACAGGGA TTTTGGAAAC AGCAAGAATT CGAAGGCTTG GTTATTCACA TCGGATACTC 
TTCGCTAACT TTATAAAGAG GTATTACATT CTCTGCTACA AATCGAGTGA AGAGCCCCCA 
GTGAGCCCAG ACACCTGTGC TGCCATTTTG GAAAAGGCTG GTCTTGATAA CTGGGCTCTT 
GGAAAAACGA AAGTGTTCCT TAAATATTAT CATGTGGAAC AGTTGAATCT AATGCGAAAG 
GAAGCTACCA ACAAGCTTGT TTTGATTCAA GCCAGTGTCA GAGCATTCTT GGGTGCAAGA 
AGATACCAAG AACTTCAGCA GAAGAGGAAG AGTAGTGCTG TAATAATACA GTCAGCTGCA 
AGAAGACATC TCTTGCGAAA ACATGGAAAA GAAACATCTA ATGTGAAAAG TGCAGCAGGA 
AGGAGCATTC GGGCTAAGGA TCAGGAATTT GACTACAAGA AAAATTTTGA AACCAAAAGG 
GAATCTTTTG TGAAGAAACA GACAGAAAAC GCAGTGCCTA CTAATGAATC CAACACTTCA 
ACTCCAAACA ACAAAGAGAG CCCATCTGCT GGGAAGACAG CTCCCTTCAG AGCAGAGTCA 
AAGGCCACCA ATGTTGAGAG TAACAACAGA AGATACCATA CTCAGAAGAA AATGAGCAAT 
GTGTATGCAG AAGGACAGAA TCAAGAGCTA TATATTGTGG AGGACACTTG GGCAGAAGTA 
AGCCCCAGGC AGAAGTATGT GCAAGACCTG GAAGAAAGCA GAAAGATGAG GAAAGAGGAG 
AAAGGAGATG CTGTGATCCA GAGTTACTGT CAGTGGTACA CAGAGGGAAG CAACTTTGAA 
GAATCAAAAG CAACATGTCT AGAAGGAAGG GAGACATGGG AAAGGACAAG CTGCCCAGGG 
CTGTGGCTGA CTGAGGAGAT TTACCTGAGA AAAACTTTGG ATCCTACTCT TAGCCAAAAG 
TCAGTCTATC AAAATGCAGA TGGCAAAGAA AAAGAACATA AAGTATCTGT GGTTACCCAG 
AATGCACCGC TGGGCAACCT GGAGAGAGAC TATCACTTGC TAGGGTTCCT TGGAGAAGAA 
GACACTGGAC CAGTGCCTCA GGCCCAGGAA GAACACAAGG CTGTGTCCAT TCATAGCAAA 
TACCAGAGTT CCAAGAAAAA GCAACAGCTG GGAAAGGACA GGCTGGCACC TCCCTTTAAG 
AATCAAAAGA TCCTTTCATC GTCCACAGAA GTAGCAAAAA CCACTCACAA TGTCTATCCC 
TGTCCGACAA AGCAGGAGGG AGTCCATCAT AGCAAGATGG TGGATGAGAG AGACTCAAAA 
ATGGCTTCAA AAAAAGAAGC GTGGGATTTA GCAATGTTTT CAAGACAGAT ATCAAAGTTA 
TCTGAAGAGT ATTTCATTCT GCAGAAAAAC TTGAATGAAA TTATTTTGGC CCAGCAACTG 
AAGCCATTTT ATCTAGGTAT CTATCGCCAT AAACCAATCA ACAGACATGT TTCTACTCAC 
CAATACCTCT CAGGTGTCTC TAAAGGGGAA GAACCAAAAA TACTGAGACC TCCAAGACGA 
CCTCGGAAAC CCAAAACATT AAATAACCCT GAAGACTCCA CATACTACTA TCTACTCCAT 
AAGTCAACCC AAGAAGAAAA ACGGAGACCA GGAAAAGACA GTCAAGGGAA GTTATTAGGT 
TTGGAAGATT TCTATTATAA GGAATTTTTG CCTACTCATT ACGGACCCAA GGCACATAGC 
TCTAATGCAA GAGAATGGAA AGCACTGAAG GAACCCCAGG CTCAACCCAT TGAGAGTAAT 
GAGAGGTGTT GGACCACCTC AGAGAATGAG AGCCTGGAGG AAGAGAGGAT TTCAGCCAAC 
CCTTATGACT ACAGGAGGCT CCTGCGCAAA ACCTCCCAGC GGCAGCGCCT GGTGCAGCAG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_680779.3
CDS	134..4999
Translation	MQMTETFEMLPLIGKTIIFDNFPDPSDTWEIIETIGKGTYGKVFKVLNKKSGQKAAVKILDPIHDIDEEIEAEYNILRTLSDHPNVVRFYGIYFKKDKINGDKLWLVLELCNGGSVTDLVKGFLKRGERMSEPVIAYILHEALMGLQHLHSNKTIHRDVKGNNILLTTEGGVKLVDFGVSAQLSSTRHRLNTSVGTPFWMAPEVIACEQQLDTTYDARCDTWSLGITAIELGDGDPPLAELHPMRALFKIPRNPPPKLRQPELWSAEFNDFISKCLTKDYEKRPTVSDLLKHKFITQIEGKDVILQKQLMEFIDIHQCLGSTEKARHERIHTKKGNLNRSLISSLKDVDDLATLDVLDENTVSEHLEKCYSRDQIYIYVGDILIALNPFQSLGIYSTKLSRLYIGAKRTANPPHIFAMADLGYQSMVTYNADQCIVISGESGAGKTESAHLLVQQLTVLGKANNRTLQEKILQMNNLVEAFGNACTIINDNSSRFGKYLEMKFTSSGAVVGAQISEYLLEKSRVIHQAIGEKNFHIFYYIYAGLAEKKKLALYKLPENKPPRYLQNDNLRTVQDMMNNSFYKSQYELIEQCFKVIGFTMEQLGSVYSVLAAILNVGNIEFSSVATEYQMDKSYICNHTALENSASLLCIQADELQEALTSHCVVTRGETIIRPNTVEKAADVRDAMAKTLYGRLFSWIVNCINSLLKHDTSPSGDEELNIGILDIFGFENFKRNSFEQLCINIANEQIQYYFNQHVFAWEQNEYLNEDVDARVIEYEDNRPLLDMFLQKPMGLLSLLDEESRFPKATDQTLIEKFEDNLKSQYFWRPKRMELSFGIHHYAGKVLYSASGFLAKNRDTFPTDIVLLLRSSENSVIRQLVNHPLTKTGNLPLSKTKNIVNYQMWNSEKSTNLTKGETRDVTCHACETTNVKTQTVSSYFRYSLMDLLSKMVVGQPHFVRCIKPNNERQARKYDKEKVLLQLRCTGILETARIRRLGYSHRILFANFIKRYYILCYKSSEEPPVSPDTCAAILEKAGLDNWALGKTKVFLKYYHVEQLNLMRKEATNKLVLIQASVRAFLGARRYQELQQKRKSSAVIIQSAARRHLLRKHGKETSNVKSAAGRSIRAKDQEFDYKKNFETKRESFVKKQTENAVPTNESNTSTPNNKESPSAGKTAPFRAESKATNVESNNRRYHTQKKMSNVYAEGQNQELYIVEDTWAEVSPRQKYVQDLEESRKMRKEEKGDAVIQSYCQWYTEGSNFEESKATCLEGRETWERTSCPGLWLTEEIYLRKTLDPTLSQKSVYQNADGKEKEHKVSVVTQNAPLGNLERDYHLLGFLGEEDTGPVPQAQEEHKAVSIHSKYQSSKKKQQLGKDRLAPPFKNQKILSSSTEVAKTTHNVYPCPTKQEGVHHSKMVDERDSKMASKKEAWDLAMFSRQISKLSEEYFILQKNLNEIILAQQLKPFYLGIYRHKPINRHVSTHQYLSGVSKGEEPKILRPPRRPRKPKTLNNPEDSTYYYLLHKSTQEEKRRPGKDSQGKLLGLEDFYYKEFLPTHYGPKAHSSNAREWKALKEPQAQPIESNERCWTTSENESLEEERISANPYDYRRLLRKTSQRQRLVQQL

Target ORF information:

RefSeq Version	NM_148413.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IIIA (Myo3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148413.3

ORF Insert Sequence:

ATGCAGATGA CAGAAACCTT TGAGATGCTT CCATTAATTG GAAAAACAAT CATCTTTGAT 
AACTTTCCTG ATCCTTCTGA TACATGGGAA ATCATTGAAA CCATCGGCAA AGGAACTTAT 
GGGAAGGTTT TCAAAGTCCT GAATAAGAAA AGTGGCCAGA AAGCAGCAGT CAAAATTTTG 
GATCCAATCC ATGATATCGA TGAAGAGATT GAAGCAGAGT ATAACATCTT AAGAACACTT 
TCTGACCACC CAAATGTGGT CAGATTCTAC GGGATTTACT TTAAAAAGGA CAAGATAAAT 
GGAGATAAGC TATGGCTGGT TCTGGAGCTG TGCAATGGAG GATCAGTGAC TGATCTTGTG 
AAAGGATTTC TGAAGAGGGG AGAAAGAATG AGCGAGCCTG TAATTGCATA TATTTTACAC 
GAAGCACTAA TGGGACTTCA GCATCTTCAT AGCAACAAAA CCATCCACCG TGATGTCAAA 
GGAAATAACA TCCTGTTGAC TACTGAAGGT GGAGTAAAAC TAGTAGATTT TGGTGTGTCT 
GCACAGCTCA GCAGCACCCG GCACCGCCTG AACACCTCCG TAGGAACACC ATTTTGGATG 
GCTCCTGAGG TGATTGCTTG TGAACAGCAA CTAGATACCA CTTACGATGC TAGATGTGAC 
ACATGGTCCC TGGGTATCAC GGCTATTGAG CTTGGTGATG GAGATCCTCC ACTAGCTGAG 
CTGCATCCCA TGAGAGCACT CTTCAAAATA CCAAGGAATC CACCCCCTAA ACTCCGGCAG 
CCTGAGCTGT GGTCCGCAGA GTTCAATGAC TTCATTAGCA AATGCTTGAC CAAAGATTAT 
GAAAAGCGTC CAACAGTGTC AGATCTTTTG AAGCATAAAT TTATTACTCA GATCGAGGGC 
AAAGATGTGA TACTACAAAA ACAACTAATG GAATTCATTG ATATCCATCA ATGCCTGGGA 
AGCACAGAGA AGGCCAGGCA TGAACGAATT CATACCAAGA AAGGTAATTT AAATAGATCT 
CTCATTTCCA GTCTGAAGGA TGTAGACGAT TTGGCAACCC TAGATGTTCT GGATGAGAAC 
ACAGTGTCTG AACATCTTGA GAAATGTTAT TCCAGAGATC AGATCTATAT TTATGTGGGA 
GATATTCTCA TTGCACTGAA CCCTTTTCAG AGTCTGGGTA TTTATTCCAC AAAGCTTTCT 
AGGTTGTATA TTGGAGCAAA GAGAACTGCC AACCCACCTC ACATTTTTGC AATGGCTGAT 
TTGGGATACC AGTCTATGGT CACATATAAT GCAGACCAGT GCATCGTTAT TTCTGGAGAA 
AGTGGTGCTG GCAAGACAGA GAGCGCTCAT CTTCTGGTTC AACAGCTCAC TGTGCTTGGA 
AAGGCTAATA ACAGAACCCT GCAAGAGAAG ATTTTACAAA TGAACAATTT GGTTGAAGCC 
TTTGGCAATG CCTGCACTAT TATAAATGAC AACTCCAGCA GATTTGGAAA ATACTTAGAA 
ATGAAATTTA CTTCTTCTGG AGCTGTAGTT GGGGCACAGA TTTCTGAATA CCTTCTGGAA 
AAATCCCGTG TCATTCACCA AGCCATTGGA GAAAAAAATT TTCATATTTT TTACTACATT 
TATGCTGGGT TAGCAGAAAA GAAGAAACTA GCTCTTTACA AATTGCCTGA AAACAAGCCA 
CCCAGGTACT TACAAAATGA CAACCTCAGA ACAGTACAGG ACATGATGAA CAATAGTTTC 
TATAAATCCC AGTATGAATT AATTGAACAA TGTTTCAAAG TCATAGGTTT TACAATGGAG 
CAACTTGGAA GTGTATATAG TGTACTCGCC GCCATCTTGA ACGTTGGAAA CATTGAATTC 
TCCTCGGTGG CAACTGAATA CCAGATGGAC AAGAGCTACA TTTGCAATCA CACTGCTCTG 
GAAAACTCCG CTTCTTTGCT TTGCATTCAA GCAGATGAGC TGCAGGAAGC TCTCACCTCA 
CACTGTGTGG TCACTAGAGG AGAAACAATT ATTCGACCCA ACACAGTGGA AAAGGCTGCT 
GATGTCCGAG ATGCCATGGC CAAAACTTTA TATGGCCGTC TCTTTAGCTG GATAGTCAAT 
TGTATTAACA GCTTGCTGAA GCATGACACA TCACCTAGTG GGGATGAGGA GCTAAACATC 
GGCATTCTTG ACATATTCGG CTTTGAAAAC TTCAAAAGAA ATTCCTTTGA GCAGCTGTGC 
ATTAACATTG CAAATGAACA GATCCAGTAT TATTTTAATC AGCACGTGTT TGCATGGGAG 
CAGAATGAAT ACCTAAATGA AGATGTAGAT GCTAGAGTTA TTGAATACGA AGACAACCGG 
CCCCTCTTGG ACATGTTTCT ACAGAAGCCC ATGGGCTTAT TGTCTCTGCT TGATGAGGAA 
AGCAGATTTC CCAAGGCCAC TGACCAGACT CTCATAGAAA AATTTGAAGA CAACCTGAAG 
TCACAGTACT TCTGGAGACC CAAAAGAATG GAACTCAGTT TTGGCATTCA CCACTATGCA 
GGAAAAGTCC TCTATAGTGC TAGTGGGTTC TTGGCTAAAA ACAGAGACAC TTTCCCAACT 
GATATTGTGC TGCTTTTGAG GTCATCAGAA AACAGTGTAA TTCGGCAACT TGTCAACCAC 
CCTCTGACCA AAACAGGTAA TCTGCCACTT TCTAAAACTA AAAACATAGT AAACTATCAG 
ATGTGGAATT CAGAGAAATC CACAAACCTG ACCAAGGGTG AAACTAGAGA TGTCACATGC 
CATGCCTGTG AAACCACCAA TGTGAAAACA CAAACGGTCT CATCCTATTT TAGATACTCC 
TTGATGGATT TATTATCTAA AATGGTAGTG GGCCAACCTC ATTTTGTCCG TTGCATCAAA 
CCAAATAATG AGCGACAGGC AAGAAAGTAT GACAAGGAGA AAGTTCTGCT CCAGCTGCGT 
TGCACAGGGA TTTTGGAAAC AGCAAGAATT CGAAGGCTTG GTTATTCACA TCGGATACTC 
TTCGCTAACT TTATAAAGAG GTATTACATT CTCTGCTACA AATCGAGTGA AGAGCCCCCA 
GTGAGCCCAG ACACCTGTGC TGCCATTTTG GAAAAGGCTG GTCTTGATAA CTGGGCTCTT 
GGAAAAACGA AAGTGTTCCT TAAATATTAT CATGTGGAAC AGTTGAATCT AATGCGAAAG 
GAAGCTACCA ACAAGCTTGT TTTGATTCAA GCCAGTGTCA GAGCATTCTT GGGTGCAAGA 
AGATACCAAG AACTTCAGCA GAAGAGGAAG AGTAGTGCTG TAATAATACA GTCAGCTGCA 
AGAAGACATC TCTTGCGAAA ACATGGAAAA GAAACATCTA ATGTGAAAAG TGCAGCAGGA 
AGGAGCATTC GGGCTAAGGA TCAGGAATTT GACTACAAGA AAAATTTTGA AACCAAAAGG 
GAATCTTTTG TGAAGAAACA GACAGAAAAC GCAGTGCCTA CTAATGAATC CAACACTTCA 
ACTCCAAACA ACAAAGAGAG CCCATCTGCT GGGAAGACAG CTCCCTTCAG AGCAGAGTCA 
AAGGCCACCA ATGTTGAGAG TAACAACAGA AGATACCATA CTCAGAAGAA AATGAGCAAT 
GTGTATGCAG AAGGACAGAA TCAAGAGCTA TATATTGTGG AGGACACTTG GGCAGAAGTA 
AGCCCCAGGC AGAAGTATGT GCAAGACCTG GAAGAAAGCA GAAAGATGAG GAAAGAGGAG 
AAAGGAGATG CTGTGATCCA GAGTTACTGT CAGTGGTACA CAGAGGGAAG CAACTTTGAA 
GAATCAAAAG CAACATGTCT AGAAGGAAGG GAGACATGGG AAAGGACAAG CTGCCCAGGG 
CTGTGGCTGA CTGAGGAGAT TTACCTGAGA AAAACTTTGG ATCCTACTCT TAGCCAAAAG 
TCAGTCTATC AAAATGCAGA TGGCAAAGAA AAAGAACATA AAGTATCTGT GGTTACCCAG 
AATGCACCGC TGGGCAACCT GGAGAGAGAC TATCACTTGC TAGGGTTCCT TGGAGAAGAA 
GACACTGGAC CAGTGCCTCA GGCCCAGGAA GAACACAAGG CTGTGTCCAT TCATAGCAAA 
TACCAGAGTT CCAAGAAAAA GCAACAGCTG GGAAAGGACA GGCTGGCACC TCCCTTTAAG 
AATCAAAAGA TCCTTTCATC GTCCACAGAA GTAGCAAAAA CCACTCACAA TGTCTATCCC 
TGTCCGACAA AGCAGGAGGG AGTCCATCAT AGCAAGATGG TGGATGAGAG AGACTCAAAA 
ATGGCTTCAA AAAAAGAAGC GTGGGATTTA GCAATGTTTT CAAGACAGAT ATCAAAGTTA 
TCTGAAGAGT ATTTCATTCT GCAGAAAAAC TTGAATGAAA TTATTTTGGC CCAGCAACTG 
AAGCCATTTT ATCTAGGTAT CTATCGCCAT AAACCAATCA ACAGACATGT TTCTACTCAC 
CAATACCTCT CAGGTGTCTC TAAAGGGGAA GAACCAAAAA TACTGAGACC TCCAAGACGA 
CCTCGGAAAC CCAAAACATT AAATAACCCT GAAGACTCCA CATACTACTA TCTACTCCAT 
AAGTCAACCC AAGAAGAAAA ACGGAGACCA GGAAAAGACA GTCAAGGGAA GTTATTAGGT 
TTGGAAGATT TCTATTATAA GGAATTTTTG CCTACTCATT ACGGACCCAA GGCACATAGC 
TCTAATGCAA GAGAATGGAA AGCACTGAAG GAACCCCAGG CTCAACCCAT TGAGAGTAAT 
GAGAGGTGTT GGACCACCTC AGAGAATGAG AGCCTGGAGG AAGAGAGGAT TTCAGCCAAC 
CCTTATGACT ACAGGAGGCT CCTGCGCAAA ACCTCCCAGC GGCAGCGCCT GGTGCAGCAG 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stereocilia-staircase spacing is influenced by myosin III motors and their cargos espin-1 and espin-like.
Nature communications710833(2016 Mar)
Ebrahim S,Avenarius MR,Grati M,Krey JF,Windsor AM,Sousa AD,Ballesteros A,Cui R,Millis BA,Salles FT,Baird MA,Davidson MW,Jones SM,Choi D,Dong L,Raval MH,Yengo CM,Barr-Gillespie PG,Kachar B

Class III myosins shape the auditory hair bundles by limiting microvilli and stereocilia growth.
The Journal of cell biology212(2)231-44(2016 Jan)
Lelli A,Michel V,Boutet de Monvel J,Cortese M,Bosch-Grau M,Aghaie A,Perfettini I,Dupont T,Avan P,El-Amraoui A,Petit C

Myosin IIIB uses an actin-binding motif in its espin-1 cargo to reach the tips of actin protrusions.
Current biology : CB22(4)320-5(2012 Feb)
Merritt RC,Manor U,Salles FT,Grati M,Dose AC,Unrath WC,Quintero OA,Yengo CM,Kachar B

Mouse class III myosins: kinase activity and phosphorylation sites.
Journal of neurochemistry119(4)772-84(2011 Nov)
Dalal JS,Stevens SM Jr,Alvarez S,Munoz N,Kempler KE,Dos? AC,Burnside B,Battelle BA

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Myo3a cDNA ORF clone, Mus musculus(house mouse)

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