Cypt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cypt1
Entrez Gene ID	66742
Full Name	cysteine-rich perinuclear theca 1
Synonyms	4931420D14Rik,Ckt1r3,Cypt5,Cypt6,TISP55
General protein information	Preferred Names cysteine-rich perinuclear theca 1 Names cysteine-rich perinuclear theca protein 1 basic protein CKT1R3 cysteine-rich perinuclear theca 5 cysteine-rich perinuclear theca 6 cysteine-rich postacrosomal PT protein cysteine-rich postacrosomal perinuclear theca protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X A1.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001310461.1	NP_001297390.1	cysteine-rich perinuclear theca protein 1 isoform 2
NM_025738.3	NP_080014.2	cysteine-rich perinuclear theca protein 1 isoform 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A shared promoter region suggests a common ancestor for the human VCX/Y, SPANX, and CSAG gene families and the murine CYPT family.
Hansen MA, Nielsen JE, Retelska D, Larsen N, Leffers H
Molecular reproduction and development75(2)219-29(2008 Feb)

Identification and expression profiling of 10 novel spermatid expressed CYPT genes.
Hansen MA, Nielsen JE, Tanaka M, Almstrup K, Skakkebaek NE, Leffers H
Molecular reproduction and development73(5)568-79(2006 May)

Characterization of a novel postacrosomal perinuclear theca-specific protein, CYPT1.
Kitamura K, Iguchi N, Kaneko Y, Tanaka H, Nishimune Y
Biology of reproduction71(6)1927-35(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Human VCX/Y, SPANX, and CSAG2 gene families together with the murine SPANX gene and the CYPT family may share a common ancestor.
Title: A shared promoter region suggests a common ancestor for the human VCX/Y, SPANX, and CSAG gene families and the murine CYPT family.

CYPT1 and the proteins of the paralogous genes constitute a novel, basic cysteine-rich sperm protein family that may contribute to the function of the postacrosomal PT during nuclear shaping.
Title: Characterization of a novel postacrosomal perinuclear theca-specific protein, CYPT1.

The following Cypt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cypt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00967	NM_025738.3 Latest version!	Mus musculus cysteine-rich perinuclear theca 1 (Cypt1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu69999	NM_001310461.1 Latest version!	Mus musculus cysteine-rich perinuclear theca 1 (Cypt1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cypt1 ( NM_025738.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080014.2 Mus musculus cysteine-rich perinuclear theca 1 (Cypt1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00967
Clone ID Related Accession (Same CDS sequence)	NM_025738.3
Accession Version	NM_025738.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	cysteine-rich perinuclear theca protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL805907.5 and AK016467.1. On Jul 14, 2015 this sequence version replaced NM_025738.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: CA463063.1, AK016467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGA TGGCCAAGAA AGTCCACTGG TCTAGCGCAG CAGCAGGAGC AGCCGCTGCT 
GCCAAAATTT CGAAGCTTGA GAAGACCACC AAAAGATTCA AACTTATTAA GAAGCGAAAC 
CCTAGTTCTA AGCTTCCCAA GAGATCTTCA CACTCTTTAC TTTGTTCTCT TTCTCGTTCC 
TGTTGTTGCT GTCGCTGTCG TTGTTGCTGT TACTGTCGTT GCTGTCGTTG TTGCTGTAGT 
CGTTCTCGTC GTTTTCGTAG CAGAACTACA TTAAAGGTGA GAGATCCCAA GTTCTTTCAG 
ATTACGGAGA AGGGGGAGCA ATCACTTCAA AGAAGAATTA GGAGACAATT GACGCGGAGC 
CAACTGGAGC TGATCGAACC GGAACCGACC ATGGCTTTGG AGCCAAGCGA GATTACAGTG 
GCATTCTTCT CTCATAAGAA TGCCAATGTG TCTGATCCAG AGGAAGTTCC ACCATGCCTT 
GACAGTGACC CATTTCCGAA TGGAGACTTG GCCAGTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080014.2
CDS	25..546
Translation	MAQMAKKVHWSSAAAGAAAAAKISKLEKTTKRFKLIKKRNPSSKLPKRSSHSLLCSLSRSCCCCRCRCCCYCRCCRCCCSRSRRFRSRTTLKVRDPKFFQITEKGEQSLQRRIRRQLTRSQLELIEPEPTMALEPSEITVAFFSHKNANVSDPEEVPPCLDSDPFPNGDLASS

Target ORF information:

RefSeq Version	NM_025738.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteine-rich perinuclear theca 1 (Cypt1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025738.3

ORF Insert Sequence:

ATGGCTCAGA TGGCCAAGAA AGTCCACTGG TCTAGCGCAG CAGCAGGAGC AGCCGCTGCT 
GCCAAAATTT CGAAGCTTGA GAAGACCACC AAAAGATTCA AACTTATTAA GAAGCGAAAC 
CCTAGTTCTA AGCTTCCCAA GAGATCTTCA CACTCTTTAC TTTGTTCTCT TTCTCGTTCC 
TGTTGTTGCT GTCGCTGTCG TTGTTGCTGT TACTGTCGTT GCTGTCGTTG TTGCTGTAGT 
CGTTCTCGTC GTTTTCGTAG CAGAACTACA TTAAAGGTGA GAGATCCCAA GTTCTTTCAG 
ATTACGGAGA AGGGGGAGCA ATCACTTCAA AGAAGAATTA GGAGACAATT GACGCGGAGC 
CAACTGGAGC TGATCGAACC GGAACCGACC ATGGCTTTGG AGCCAAGCGA GATTACAGTG 
GCATTCTTCT CTCATAAGAA TGCCAATGTG TCTGATCCAG AGGAAGTTCC ACCATGCCTT 
GACAGTGACC CATTTCCGAA TGGAGACTTG GCCAGTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cypt1 ( NM_001310461.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297390.1 Mus musculus cysteine-rich perinuclear theca 1 (Cypt1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69999
Clone ID Related Accession (Same CDS sequence)	NM_001310461.1
Accession Version	NM_001310461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	cysteine-rich perinuclear theca protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK006892.1 and AL805907.5. On Jul 14, 2015 this sequence version replaced XM_006527692.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK006892.1, BC061079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGA TGGCCAAGAA AGTCCACTGG TCTAGCGCAG CAGCAGGAGC AGCCGCTGCT 
GCCAAAATTT CGAAGCTTGA GAAGACCACC AAAAGATTCA AACTTATTAA GAAGCGAAAC 
CCTAGTTCTA AGCTTCCCAA GAGATCTTCA CACTCTTTAC TTTGTTCTCT TTCTCGTTCC 
TGTTGTTGCT GTCGCTGTCG TTGTTGCTGT TACTGTCGTT GCTGTCGTTG TTGCTGTAGT 
CGTTCTCGTC GTTTTCGTAG CAGAACTACA TTAAAGTTCT TTCAGATTAC GGAGAAGGGG 
GAGCAATCAC TTCAAAGAAG AATTAGGAGA CAATTGACGC GGAGCCAACT GGAGCTGATC 
GAACCGGAAC CGACCATGGC TTTGGAGCCA AGCGAGATTA CAGTGGCATT CTTCTCTCAT 
AAGAATGCCA ATGTGTCTGA TCCAGAGGAA GTTCCACCAT GCCTTGACAG TGACCCATTT 
CCGAATGGAG ACTTGGCCAG TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297390.1
CDS	25..531
Translation	MAQMAKKVHWSSAAAGAAAAAKISKLEKTTKRFKLIKKRNPSSKLPKRSSHSLLCSLSRSCCCCRCRCCCYCRCCRCCCSRSRRFRSRTTLKFFQITEKGEQSLQRRIRRQLTRSQLELIEPEPTMALEPSEITVAFFSHKNANVSDPEEVPPCLDSDPFPNGDLASS

Target ORF information:

RefSeq Version	NM_001310461.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cysteine-rich perinuclear theca 1 (Cypt1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310461.1

ORF Insert Sequence:

ATGGCTCAGA TGGCCAAGAA AGTCCACTGG TCTAGCGCAG CAGCAGGAGC AGCCGCTGCT 
GCCAAAATTT CGAAGCTTGA GAAGACCACC AAAAGATTCA AACTTATTAA GAAGCGAAAC 
CCTAGTTCTA AGCTTCCCAA GAGATCTTCA CACTCTTTAC TTTGTTCTCT TTCTCGTTCC 
TGTTGTTGCT GTCGCTGTCG TTGTTGCTGT TACTGTCGTT GCTGTCGTTG TTGCTGTAGT 
CGTTCTCGTC GTTTTCGTAG CAGAACTACA TTAAAGTTCT TTCAGATTAC GGAGAAGGGG 
GAGCAATCAC TTCAAAGAAG AATTAGGAGA CAATTGACGC GGAGCCAACT GGAGCTGATC 
GAACCGGAAC CGACCATGGC TTTGGAGCCA AGCGAGATTA CAGTGGCATT CTTCTCTCAT 
AAGAATGCCA ATGTGTCTGA TCCAGAGGAA GTTCCACCAT GCCTTGACAG TGACCCATTT 
CCGAATGGAG ACTTGGCCAG TTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A shared promoter region suggests a common ancestor for the human VCX/Y, SPANX, and CSAG gene families and the murine CYPT family.
Molecular reproduction and development75(2)219-29(2008 Feb)
Hansen MA,Nielsen JE,Retelska D,Larsen N,Leffers H

Identification and expression profiling of 10 novel spermatid expressed CYPT genes.
Molecular reproduction and development73(5)568-79(2006 May)
Hansen MA,Nielsen JE,Tanaka M,Almstrup K,Skakkebaek NE,Leffers H

Characterization of a novel postacrosomal perinuclear theca-specific protein, CYPT1.
Biology of reproduction71(6)1927-35(2004 Dec)
Kitamura K,Iguchi N,Kaneko Y,Tanaka H,Nishimune Y

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Cypt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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