Ap3m2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap3m2
Entrez Gene ID	64933
Full Name	adaptor-related protein complex 3, mu 2 subunit
Synonyms	5830445E16Rik,AP-3B
General protein information	Preferred Names adaptor-related protein complex 3, mu 2 subunit Names AP-3 complex subunit mu-2 HA1 47 kDa subunit homolog 2 P47B adapter-related protein complex 3 mu-2 subunit adapter-related protein complex 3 subunit mu-2 adaptor-related protein complex 3 subunit mu-2 clathrin assembly protein assembly protein complex 1 medium chain homolog 2 clathrin assembly protein assembly protein complex 3 mu-2 medium chain clathrin coat assembly protein AP47 homolog 2 clathrin coat-associated protein AP47 homolog 2 golgi adaptor AP-1 47 kDa protein homolog 2 m3B mu3B-adaptin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_029505.3	NP_083781.2	AP-3 complex subunit mu-2
NM_001122820.1	NP_001116292.1	AP-3 complex subunit mu-2
XM_030243706.1	XP_030099566.1	AP-3 complex subunit mu-2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AP3M2	XP_001141415.1
Arabidopsis thaliana (thale cress)	ZIP4	NP_176052.3
Bos taurus (cattle)	AP3M2	NP_001180169.1
Mus musculus (house mouse)	Ap3m2	NP_001116292.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001484	XP_321638.4
Oryza sativa (rice)	Os05g0383100	NP_001055410.1
Xenopus tropicalis (tropical clawed frog)	ap3m2	NP_001016650.1
Homo sapiens (human)	AP3M2	NP_001127768.1
Rattus norvegicus (Norway rat)	Ap3m2	NP_579839.1
Gallus gallus (chicken)	AP3M2	XP_424403.2
Drosophila melanogaster (fruit fly)	cm	NP_001259302.1
Canis lupus familiaris (dog)	AP3M2	XP_005629863.1
Danio rerio (zebrafish)	ap3m2	NP_001002154.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a QTL in Mus musculus for alcohol preference, withdrawal, and Ap3m2 expression using integrative functional genomics and precision genetics.
Bubier JA, Jay JJ, Baker CL, Bergeson SE, Ohno H, Metten P, Crabbe JC, Chesler EJ
Genetics197(4)1377-93(2014 Aug)

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.
Larimore J, Tornieri K, Ryder PV, Gokhale A, Zlatic SA, Craige B, Lee JD, Talbot K, Pare JF, Smith Y, Faundez V
Molecular biology of the cell22(24)4854-67(2011 Dec)

Aberrant trafficking of the high-affinity choline transporter in AP-3-deficient mice.
Misawa H, Fujigaya H, Nishimura T, Moriwaki Y, Okuda T, Kawashima K, Nakata K, Ruggiero AM, Blakely RD, Nakatsu F, Ohno H
The European journal of neuroscience27(12)3109-17(2008 Jun)

Defective function of GABA-containing synaptic vesicles in mice lacking the AP-3B clathrin adaptor.
Nakatsu F, Okada M, Mori F, Kumazawa N, Iwasa H, Zhu G, Kasagi Y, Kamiya H, Harada A, Nishimura K, Takeuchi A, Miyazaki T, Watanabe M, Yuasa S, Manabe T, Wakabayashi K, Kaneko S, Saito T, Ohno H
The Journal of cell biology167(2)293-302(2004 Oct)

The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes.
Blumstein J, Faundez V, Nakatsu F, Saito T, Ohno H, Kelly RB
The Journal of neuroscience : the official journal of the Society for Neuroscience21(20)8034-42(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We have demonstrated genetic and alcohol-induced regulation of Ap3m2 expression. Although sequence analysis revealed no polymorphisms in the Ap3m2-coding region that could account for all phenotypic differences, there are several upstream SNPs that could.
Title: Identification of a QTL in Mus musculus for alcohol preference, withdrawal, and Ap3m2 expression using integrative functional genomics and precision genetics.

The following Ap3m2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap3m2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05754	NM_001122820.1 Latest version!	Mus musculus adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu05754	NM_029505.3 Latest version!	Mus musculus adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu05754	XM_030243706.1 Latest version!	Mus musculus adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap3m2 ( NM_029505.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083781.2 Mus musculus adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05754
Clone ID Related Accession (Same CDS sequence)	NM_029505.3 , NM_001122820.1 , XM_030243706.1
Accession Version	NM_029505.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	AP-3 complex subunit mu-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB610934.1 and AK081220.1. On Mar 25, 2008 this sequence version replaced NM_029505.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK081220.1, SRR1660817.279967.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTCACA GCCTTTTCTT GATTAACTCT TCCGGAGATA TTTTCCTGGA GAAACACTGG 
AAAAGTGTGG TCAGCCGCTC TGTGTGTGAT TACTTCTTCG AGGCCCAAGA GAGAGCTACC 
GAGGCAGAAA ATGTGCCTCC GGTCATCCCC ACCCCTCACC ACTACCTCCT GAGTGTTTAC 
CGGCACAAGA TCTTCTTTGT GGCTGTAATC CAGACAGAGG TCCCGCCTCT GTTTGTCATT 
GAATTCCTTC ACCGAGTAGT GGATACATTT CAGGACTATT TTGGGGTCTG TTCCGAGCCA 
GTGATCAAAG ACAATGTGGT GGTGGTGTAC GAGGTGTTGG AAGAGATGCT GGACAACGGG 
TTCCCCTTGG CTACGGAGTC AAACATCCTC AAAGAACTGA TAAAGCCTCC CACTATTCTC 
CGGACAGTGG TCAACACCAT CACAGGAAGC ACCAATGTGG GGGACCAGCT TCCAACTGGG 
CAGCTGTCGG TGGTGCCTTG GCGACGGACA GGGGTGAAAT ATACCAACAA CGAGGCCTAT 
TTCGATGTGG TGGAAGAGAT AGACGCCATC ATTGACAAGT CAGGTTCCAC AGTCACCGCT 
GAGATCCAAG GGGTGATCGA TGCCTGTGTT AAGCTGACCG GGATGCCGGA TCTCACTCTG 
TCCTTCATGA ACCCCAGGTT GCTGGACGAT GTTAGCTTCC ACCCCTGTGT CCGTTTCAAG 
CGCTGGGAAT CTGAGCGCAT CCTCTCCTTC ATCCCTCCTG ACGGCAACTT CCGCCTGCTC 
GCCTACCACG TCAGTGCACA GAACCTCGTT GCCATTCCAG TCTATGTCAA ACATAGCATC 
AGCTTCCGGG ACAGCAGTTC ACTTGGACGC TTTGAAATCA CGGTGGGACC CAAGCAGACC 
ATGGGGAAGA CCATAGAGGG TGTGATTGTC ACCAGCCAGA TGCCCAAAGG AGTCCTGAAT 
ATGAGCCTCA CTCCATCTCA GGGGACACAC ACGTTTGACC CCGTGACAAA GATGCTGTCT 
TGGGATGTAG GAAAAATAAA CCCACAGAAG CTGCCAAGTT TGAAGGGGAC GATGGGTCTC 
CAGGTCGGAG CTTCTAAACC AGATGAGAAC CCTACGATTA ACCTGCAGTT TAAGATCCAG 
CAGCTGGCCA TTTCTGGACT CAAAGTGAAC CGTCTGGATA TGTACGGGGA GAAGTACAAG 
CCCTTTAAAG GCATCAAATA CATGACCAAA GCCGGCAAGT TCCAAGTCCG AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083781.2
CDS	150..1406
Translation	MIHSLFLINSSGDIFLEKHWKSVVSRSVCDYFFEAQERATEAENVPPVIPTPHHYLLSVYRHKIFFVAVIQTEVPPLFVIEFLHRVVDTFQDYFGVCSEPVIKDNVVVVYEVLEEMLDNGFPLATESNILKELIKPPTILRTVVNTITGSTNVGDQLPTGQLSVVPWRRTGVKYTNNEAYFDVVEEIDAIIDKSGSTVTAEIQGVIDACVKLTGMPDLTLSFMNPRLLDDVSFHPCVRFKRWESERILSFIPPDGNFRLLAYHVSAQNLVAIPVYVKHSISFRDSSSLGRFEITVGPKQTMGKTIEGVIVTSQMPKGVLNMSLTPSQGTHTFDPVTKMLSWDVGKINPQKLPSLKGTMGLQVGASKPDENPTINLQFKIQQLAISGLKVNRLDMYGEKYKPFKGIKYMTKAGKFQVRT

Target ORF information:

RefSeq Version	NM_029505.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029505.3

ORF Insert Sequence:

ATGATTCACA GCCTTTTCTT GATTAACTCT TCCGGAGATA TTTTCCTGGA GAAACACTGG 
AAAAGTGTGG TCAGCCGCTC TGTGTGTGAT TACTTCTTCG AGGCCCAAGA GAGAGCTACC 
GAGGCAGAAA ATGTGCCTCC GGTCATCCCC ACCCCTCACC ACTACCTCCT GAGTGTTTAC 
CGGCACAAGA TCTTCTTTGT GGCTGTAATC CAGACAGAGG TCCCGCCTCT GTTTGTCATT 
GAATTCCTTC ACCGAGTAGT GGATACATTT CAGGACTATT TTGGGGTCTG TTCCGAGCCA 
GTGATCAAAG ACAATGTGGT GGTGGTGTAC GAGGTGTTGG AAGAGATGCT GGACAACGGG 
TTCCCCTTGG CTACGGAGTC AAACATCCTC AAAGAACTGA TAAAGCCTCC CACTATTCTC 
CGGACAGTGG TCAACACCAT CACAGGAAGC ACCAATGTGG GGGACCAGCT TCCAACTGGG 
CAGCTGTCGG TGGTGCCTTG GCGACGGACA GGGGTGAAAT ATACCAACAA CGAGGCCTAT 
TTCGATGTGG TGGAAGAGAT AGACGCCATC ATTGACAAGT CAGGTTCCAC AGTCACCGCT 
GAGATCCAAG GGGTGATCGA TGCCTGTGTT AAGCTGACCG GGATGCCGGA TCTCACTCTG 
TCCTTCATGA ACCCCAGGTT GCTGGACGAT GTTAGCTTCC ACCCCTGTGT CCGTTTCAAG 
CGCTGGGAAT CTGAGCGCAT CCTCTCCTTC ATCCCTCCTG ACGGCAACTT CCGCCTGCTC 
GCCTACCACG TCAGTGCACA GAACCTCGTT GCCATTCCAG TCTATGTCAA ACATAGCATC 
AGCTTCCGGG ACAGCAGTTC ACTTGGACGC TTTGAAATCA CGGTGGGACC CAAGCAGACC 
ATGGGGAAGA CCATAGAGGG TGTGATTGTC ACCAGCCAGA TGCCCAAAGG AGTCCTGAAT 
ATGAGCCTCA CTCCATCTCA GGGGACACAC ACGTTTGACC CCGTGACAAA GATGCTGTCT 
TGGGATGTAG GAAAAATAAA CCCACAGAAG CTGCCAAGTT TGAAGGGGAC GATGGGTCTC 
CAGGTCGGAG CTTCTAAACC AGATGAGAAC CCTACGATTA ACCTGCAGTT TAAGATCCAG 
CAGCTGGCCA TTTCTGGACT CAAAGTGAAC CGTCTGGATA TGTACGGGGA GAAGTACAAG 
CCCTTTAAAG GCATCAAATA CATGACCAAA GCCGGCAAGT TCCAAGTCCG AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap3m2 ( NM_001122820.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001116292.1 Mus musculus adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05754
Clone ID Related Accession (Same CDS sequence)	NM_029505.3 , NM_001122820.1 , XM_030243706.1
Accession Version	NM_001122820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	AP-3 complex subunit mu-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB610934.1, BY121529.1 and AK081220.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK134634.1, SRR1660813.232211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTCACA GCCTTTTCTT GATTAACTCT TCCGGAGATA TTTTCCTGGA GAAACACTGG 
AAAAGTGTGG TCAGCCGCTC TGTGTGTGAT TACTTCTTCG AGGCCCAAGA GAGAGCTACC 
GAGGCAGAAA ATGTGCCTCC GGTCATCCCC ACCCCTCACC ACTACCTCCT GAGTGTTTAC 
CGGCACAAGA TCTTCTTTGT GGCTGTAATC CAGACAGAGG TCCCGCCTCT GTTTGTCATT 
GAATTCCTTC ACCGAGTAGT GGATACATTT CAGGACTATT TTGGGGTCTG TTCCGAGCCA 
GTGATCAAAG ACAATGTGGT GGTGGTGTAC GAGGTGTTGG AAGAGATGCT GGACAACGGG 
TTCCCCTTGG CTACGGAGTC AAACATCCTC AAAGAACTGA TAAAGCCTCC CACTATTCTC 
CGGACAGTGG TCAACACCAT CACAGGAAGC ACCAATGTGG GGGACCAGCT TCCAACTGGG 
CAGCTGTCGG TGGTGCCTTG GCGACGGACA GGGGTGAAAT ATACCAACAA CGAGGCCTAT 
TTCGATGTGG TGGAAGAGAT AGACGCCATC ATTGACAAGT CAGGTTCCAC AGTCACCGCT 
GAGATCCAAG GGGTGATCGA TGCCTGTGTT AAGCTGACCG GGATGCCGGA TCTCACTCTG 
TCCTTCATGA ACCCCAGGTT GCTGGACGAT GTTAGCTTCC ACCCCTGTGT CCGTTTCAAG 
CGCTGGGAAT CTGAGCGCAT CCTCTCCTTC ATCCCTCCTG ACGGCAACTT CCGCCTGCTC 
GCCTACCACG TCAGTGCACA GAACCTCGTT GCCATTCCAG TCTATGTCAA ACATAGCATC 
AGCTTCCGGG ACAGCAGTTC ACTTGGACGC TTTGAAATCA CGGTGGGACC CAAGCAGACC 
ATGGGGAAGA CCATAGAGGG TGTGATTGTC ACCAGCCAGA TGCCCAAAGG AGTCCTGAAT 
ATGAGCCTCA CTCCATCTCA GGGGACACAC ACGTTTGACC CCGTGACAAA GATGCTGTCT 
TGGGATGTAG GAAAAATAAA CCCACAGAAG CTGCCAAGTT TGAAGGGGAC GATGGGTCTC 
CAGGTCGGAG CTTCTAAACC AGATGAGAAC CCTACGATTA ACCTGCAGTT TAAGATCCAG 
CAGCTGGCCA TTTCTGGACT CAAAGTGAAC CGTCTGGATA TGTACGGGGA GAAGTACAAG 
CCCTTTAAAG GCATCAAATA CATGACCAAA GCCGGCAAGT TCCAAGTCCG AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116292.1
CDS	212..1468
Translation	MIHSLFLINSSGDIFLEKHWKSVVSRSVCDYFFEAQERATEAENVPPVIPTPHHYLLSVYRHKIFFVAVIQTEVPPLFVIEFLHRVVDTFQDYFGVCSEPVIKDNVVVVYEVLEEMLDNGFPLATESNILKELIKPPTILRTVVNTITGSTNVGDQLPTGQLSVVPWRRTGVKYTNNEAYFDVVEEIDAIIDKSGSTVTAEIQGVIDACVKLTGMPDLTLSFMNPRLLDDVSFHPCVRFKRWESERILSFIPPDGNFRLLAYHVSAQNLVAIPVYVKHSISFRDSSSLGRFEITVGPKQTMGKTIEGVIVTSQMPKGVLNMSLTPSQGTHTFDPVTKMLSWDVGKINPQKLPSLKGTMGLQVGASKPDENPTINLQFKIQQLAISGLKVNRLDMYGEKYKPFKGIKYMTKAGKFQVRT

Target ORF information:

RefSeq Version	NM_001122820.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001122820.1

ORF Insert Sequence:

ATGATTCACA GCCTTTTCTT GATTAACTCT TCCGGAGATA TTTTCCTGGA GAAACACTGG 
AAAAGTGTGG TCAGCCGCTC TGTGTGTGAT TACTTCTTCG AGGCCCAAGA GAGAGCTACC 
GAGGCAGAAA ATGTGCCTCC GGTCATCCCC ACCCCTCACC ACTACCTCCT GAGTGTTTAC 
CGGCACAAGA TCTTCTTTGT GGCTGTAATC CAGACAGAGG TCCCGCCTCT GTTTGTCATT 
GAATTCCTTC ACCGAGTAGT GGATACATTT CAGGACTATT TTGGGGTCTG TTCCGAGCCA 
GTGATCAAAG ACAATGTGGT GGTGGTGTAC GAGGTGTTGG AAGAGATGCT GGACAACGGG 
TTCCCCTTGG CTACGGAGTC AAACATCCTC AAAGAACTGA TAAAGCCTCC CACTATTCTC 
CGGACAGTGG TCAACACCAT CACAGGAAGC ACCAATGTGG GGGACCAGCT TCCAACTGGG 
CAGCTGTCGG TGGTGCCTTG GCGACGGACA GGGGTGAAAT ATACCAACAA CGAGGCCTAT 
TTCGATGTGG TGGAAGAGAT AGACGCCATC ATTGACAAGT CAGGTTCCAC AGTCACCGCT 
GAGATCCAAG GGGTGATCGA TGCCTGTGTT AAGCTGACCG GGATGCCGGA TCTCACTCTG 
TCCTTCATGA ACCCCAGGTT GCTGGACGAT GTTAGCTTCC ACCCCTGTGT CCGTTTCAAG 
CGCTGGGAAT CTGAGCGCAT CCTCTCCTTC ATCCCTCCTG ACGGCAACTT CCGCCTGCTC 
GCCTACCACG TCAGTGCACA GAACCTCGTT GCCATTCCAG TCTATGTCAA ACATAGCATC 
AGCTTCCGGG ACAGCAGTTC ACTTGGACGC TTTGAAATCA CGGTGGGACC CAAGCAGACC 
ATGGGGAAGA CCATAGAGGG TGTGATTGTC ACCAGCCAGA TGCCCAAAGG AGTCCTGAAT 
ATGAGCCTCA CTCCATCTCA GGGGACACAC ACGTTTGACC CCGTGACAAA GATGCTGTCT 
TGGGATGTAG GAAAAATAAA CCCACAGAAG CTGCCAAGTT TGAAGGGGAC GATGGGTCTC 
CAGGTCGGAG CTTCTAAACC AGATGAGAAC CCTACGATTA ACCTGCAGTT TAAGATCCAG 
CAGCTGGCCA TTTCTGGACT CAAAGTGAAC CGTCTGGATA TGTACGGGGA GAAGTACAAG 
CCCTTTAAAG GCATCAAATA CATGACCAAA GCCGGCAAGT TCCAAGTCCG AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap3m2 ( XM_030243706.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099566.1 Mus musculus adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu05754
Clone ID Related Accession (Same CDS sequence)	NM_029505.3 , NM_001122820.1 , XM_030243706.1
Accession Version	XM_030243706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	AP-3 complex subunit mu-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCACA GCCTTTTCTT GATTAACTCT TCCGGAGATA TTTTCCTGGA GAAACACTGG 
AAAAGTGTGG TCAGCCGCTC TGTGTGTGAT TACTTCTTCG AGGCCCAAGA GAGAGCTACC 
GAGGCAGAAA ATGTGCCTCC GGTCATCCCC ACCCCTCACC ACTACCTCCT GAGTGTTTAC 
CGGCACAAGA TCTTCTTTGT GGCTGTAATC CAGACAGAGG TCCCGCCTCT GTTTGTCATT 
GAATTCCTTC ACCGAGTAGT GGATACATTT CAGGACTATT TTGGGGTCTG TTCCGAGCCA 
GTGATCAAAG ACAATGTGGT GGTGGTGTAC GAGGTGTTGG AAGAGATGCT GGACAACGGG 
TTCCCCTTGG CTACGGAGTC AAACATCCTC AAAGAACTGA TAAAGCCTCC CACTATTCTC 
CGGACAGTGG TCAACACCAT CACAGGAAGC ACCAATGTGG GGGACCAGCT TCCAACTGGG 
CAGCTGTCGG TGGTGCCTTG GCGACGGACA GGGGTGAAAT ATACCAACAA CGAGGCCTAT 
TTCGATGTGG TGGAAGAGAT AGACGCCATC ATTGACAAGT CAGGTTCCAC AGTCACCGCT 
GAGATCCAAG GGGTGATCGA TGCCTGTGTT AAGCTGACCG GGATGCCGGA TCTCACTCTG 
TCCTTCATGA ACCCCAGGTT GCTGGACGAT GTTAGCTTCC ACCCCTGTGT CCGTTTCAAG 
CGCTGGGAAT CTGAGCGCAT CCTCTCCTTC ATCCCTCCTG ACGGCAACTT CCGCCTGCTC 
GCCTACCACG TCAGTGCACA GAACCTCGTT GCCATTCCAG TCTATGTCAA ACATAGCATC 
AGCTTCCGGG ACAGCAGTTC ACTTGGACGC TTTGAAATCA CGGTGGGACC CAAGCAGACC 
ATGGGGAAGA CCATAGAGGG TGTGATTGTC ACCAGCCAGA TGCCCAAAGG AGTCCTGAAT 
ATGAGCCTCA CTCCATCTCA GGGGACACAC ACGTTTGACC CCGTGACAAA GATGCTGTCT 
TGGGATGTAG GAAAAATAAA CCCACAGAAG CTGCCAAGTT TGAAGGGGAC GATGGGTCTC 
CAGGTCGGAG CTTCTAAACC AGATGAGAAC CCTACGATTA ACCTGCAGTT TAAGATCCAG 
CAGCTGGCCA TTTCTGGACT CAAAGTGAAC CGTCTGGATA TGTACGGGGA GAAGTACAAG 
CCCTTTAAAG GCATCAAATA CATGACCAAA GCCGGCAAGT TCCAAGTCCG AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099566.1
CDS	436..1692
Translation	MIHSLFLINSSGDIFLEKHWKSVVSRSVCDYFFEAQERATEAENVPPVIPTPHHYLLSVYRHKIFFVAVIQTEVPPLFVIEFLHRVVDTFQDYFGVCSEPVIKDNVVVVYEVLEEMLDNGFPLATESNILKELIKPPTILRTVVNTITGSTNVGDQLPTGQLSVVPWRRTGVKYTNNEAYFDVVEEIDAIIDKSGSTVTAEIQGVIDACVKLTGMPDLTLSFMNPRLLDDVSFHPCVRFKRWESERILSFIPPDGNFRLLAYHVSAQNLVAIPVYVKHSISFRDSSSLGRFEITVGPKQTMGKTIEGVIVTSQMPKGVLNMSLTPSQGTHTFDPVTKMLSWDVGKINPQKLPSLKGTMGLQVGASKPDENPTINLQFKIQQLAISGLKVNRLDMYGEKYKPFKGIKYMTKAGKFQVRT

Target ORF information:

RefSeq Version	XM_030243706.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 3, mu 2 subunit (Ap3m2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243706.1

ORF Insert Sequence:

ATGATTCACA GCCTTTTCTT GATTAACTCT TCCGGAGATA TTTTCCTGGA GAAACACTGG 
AAAAGTGTGG TCAGCCGCTC TGTGTGTGAT TACTTCTTCG AGGCCCAAGA GAGAGCTACC 
GAGGCAGAAA ATGTGCCTCC GGTCATCCCC ACCCCTCACC ACTACCTCCT GAGTGTTTAC 
CGGCACAAGA TCTTCTTTGT GGCTGTAATC CAGACAGAGG TCCCGCCTCT GTTTGTCATT 
GAATTCCTTC ACCGAGTAGT GGATACATTT CAGGACTATT TTGGGGTCTG TTCCGAGCCA 
GTGATCAAAG ACAATGTGGT GGTGGTGTAC GAGGTGTTGG AAGAGATGCT GGACAACGGG 
TTCCCCTTGG CTACGGAGTC AAACATCCTC AAAGAACTGA TAAAGCCTCC CACTATTCTC 
CGGACAGTGG TCAACACCAT CACAGGAAGC ACCAATGTGG GGGACCAGCT TCCAACTGGG 
CAGCTGTCGG TGGTGCCTTG GCGACGGACA GGGGTGAAAT ATACCAACAA CGAGGCCTAT 
TTCGATGTGG TGGAAGAGAT AGACGCCATC ATTGACAAGT CAGGTTCCAC AGTCACCGCT 
GAGATCCAAG GGGTGATCGA TGCCTGTGTT AAGCTGACCG GGATGCCGGA TCTCACTCTG 
TCCTTCATGA ACCCCAGGTT GCTGGACGAT GTTAGCTTCC ACCCCTGTGT CCGTTTCAAG 
CGCTGGGAAT CTGAGCGCAT CCTCTCCTTC ATCCCTCCTG ACGGCAACTT CCGCCTGCTC 
GCCTACCACG TCAGTGCACA GAACCTCGTT GCCATTCCAG TCTATGTCAA ACATAGCATC 
AGCTTCCGGG ACAGCAGTTC ACTTGGACGC TTTGAAATCA CGGTGGGACC CAAGCAGACC 
ATGGGGAAGA CCATAGAGGG TGTGATTGTC ACCAGCCAGA TGCCCAAAGG AGTCCTGAAT 
ATGAGCCTCA CTCCATCTCA GGGGACACAC ACGTTTGACC CCGTGACAAA GATGCTGTCT 
TGGGATGTAG GAAAAATAAA CCCACAGAAG CTGCCAAGTT TGAAGGGGAC GATGGGTCTC 
CAGGTCGGAG CTTCTAAACC AGATGAGAAC CCTACGATTA ACCTGCAGTT TAAGATCCAG 
CAGCTGGCCA TTTCTGGACT CAAAGTGAAC CGTCTGGATA TGTACGGGGA GAAGTACAAG 
CCCTTTAAAG GCATCAAATA CATGACCAAA GCCGGCAAGT TCCAAGTCCG AACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of a QTL in Mus musculus for alcohol preference, withdrawal, and Ap3m2 expression using integrative functional genomics and precision genetics.
Genetics197(4)1377-93(2014 Aug)
Bubier JA,Jay JJ,Baker CL,Bergeson SE,Ohno H,Metten P,Crabbe JC,Chesler EJ

The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse.
Molecular biology of the cell22(24)4854-67(2011 Dec)
Larimore J,Tornieri K,Ryder PV,Gokhale A,Zlatic SA,Craige B,Lee JD,Talbot K,Pare JF,Smith Y,Faundez V

Aberrant trafficking of the high-affinity choline transporter in AP-3-deficient mice.
The European journal of neuroscience27(12)3109-17(2008 Jun)
Misawa H,Fujigaya H,Nishimura T,Moriwaki Y,Okuda T,Kawashima K,Nakata K,Ruggiero AM,Blakely RD,Nakatsu F,Ohno H

Defective function of GABA-containing synaptic vesicles in mice lacking the AP-3B clathrin adaptor.
The Journal of cell biology167(2)293-302(2004 Oct)
Nakatsu F,Okada M,Mori F,Kumazawa N,Iwasa H,Zhu G,Kasagi Y,Kamiya H,Harada A,Nishimura K,Takeuchi A,Miyazaki T,Watanabe M,Yuasa S,Manabe T,Wakabayashi K,Kaneko S,Saito T,Ohno H

The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes.
The Journal of neuroscience : the official journal of the Society for Neuroscience21(20)8034-42(2001 Oct)
Blumstein J,Faundez V,Nakatsu F,Saito T,Ohno H,Kelly RB

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Ap3m2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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