Kcnmb4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kcnmb4
Entrez Gene ID	58802
Full Name	potassium large conductance calcium-activated channel, subfamily M, beta member 4
Synonyms	1700058G18Rik,2900045G12Rik
General protein information	Preferred Names potassium large conductance calcium-activated channel, subfamily M, beta member 4 Names calcium-activated potassium channel subunit beta-4 BK channel subunit beta-4 BKbeta4 Slowpoke beta 4 calcium-activated potassium channel, subfamily M subunit beta-4 charybdotoxin receptor subunit beta-4 k(VCA)beta-4 large conductance calcium-dependent potassium ion channel beta 4 subunit maxi K channel subunit beta-4 slo-beta-4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 D2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006513911.4	XP_006513974.1	calcium-activated potassium channel subunit beta-4 isoform X2
XM_017314034.1	XP_017169523.1	calcium-activated potassium channel subunit beta-4 isoform X1
XM_006513911.3	XP_006513974.1	calcium-activated potassium channel subunit beta-4 isoform X2
NM_021452.1	NP_067427.1	calcium-activated potassium channel subunit beta-4
XM_017314034.2	XP_017169523.1	calcium-activated potassium channel subunit beta-4 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	KCNMB4	NP_001186559.1
Homo sapiens (human)	KCNMB4	NP_055320.4
Mus musculus (house mouse)	Kcnmb4	NP_067427.1
	LOC717360	XP_002798706.1
Canis lupus familiaris (dog)	KCNMB4	XP_005625718.1
Xenopus tropicalis (tropical clawed frog)	kcnmb4	NP_001017211.1
Pan troglodytes (chimpanzee)	KCNMB4	XP_001156630.1
Bos taurus (cattle)	KCNMB4	NP_001179452.1
Rattus norvegicus (Norway rat)	Kcnmb4	NP_076450.1

Gene Ontology
Bibliography

Related articles in PubMed

Tagging of Endogenous BK Channels with a Fluorogen-Activating Peptide Reveals ?4-Mediated Control of Channel Clustering in Cerebellum.
Pratt CP, Kuljis DA, Homanics GE, He J, Kolodieznyi D, Dudem S, Hollywood MA, Barth AL, Bruchez MP
Frontiers in cellular neuroscience11337(2017)

Knockout of the BK ?4-subunit promotes a functional coupling of BK channels and ryanodine receptors that mediate a fAHP-induced increase in excitability.
Wang B, Bugay V, Ling L, Chuang HH, Jaffe DB, Brenner R
Journal of neurophysiology116(2)456-65(2016 Aug)

Cochlear function in mice lacking the BK channel alpha, beta1, or beta4 subunits.
Pyott SJ, Meredith AL, Fodor AA, V?zquez AE, Yamoah EN, Aldrich RW
The Journal of biological chemistry282(5)3312-24(2007 Feb)

BK channel beta4 subunit reduces dentate gyrus excitability and protects against temporal lobe seizures.
Brenner R, Chen QH, Vilaythong A, Toney GM, Noebels JL, Aldrich RW
Nature neuroscience8(12)1752-9(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

conclude that seizure-induced downregulation of KCNMB4 is an activity dependent mechanism that increases the excitability of DG neurons. These novel findings indicate that BK channel subtypes are not only defined by cell-specific expression, but can also be plastic depending on the recent history of neuronal excitability
Title: Downregulation of KCNMB4 expression and changes in BK channel subtype in hippocampal granule neurons following seizure activity.

These results indicate that an important role of the beta4-subunit is to reduce ryanodine receptor-BK channel functional coupling during the afterhyperpolarization component of the action potential, thereby decreasing excitability of dentate gyrus neurons.
Title: Knockout of the BK ?4-subunit promotes a functional coupling of BK channels and ryanodine receptors that mediate a fAHP-induced increase in excitability.

the enhanced effect of amiloride on potassium secretion in wild-type compared to knockout mice on the alkaline diet clarify a BK- alpha/beta4-mediated potassium secretory pathway in intercalated cells driven by ENaC-mediated sodium reabsorption
Title: Relation between BK-?/?4-mediated potassium secretion and ENaC-mediated sodium reabsorption.

BKbeta4 expression in astrocytes likely participates in regulating astrocytic voltage gradients and maintaining potassium homeostasis, hence enabling astrocytes to fulfill their complex regulatory influence on proper brain function.
Title: Expression of the voltage- and Ca2+-dependent BK potassium channel subunits BK?1 and BK?4 in rodent astrocytes.

results argue that, for native mouse Slo3 channels, the beta4 subunit must be considered as a potential interaction partner and, furthermore, that KCNMB subunits may have functions unrelated to regulation of the Slo1 alpha subunit
Title: Interactions between beta subunits of the KCNMB family and Slo3: beta4 selectively modulates Slo3 expression and function.

The following Kcnmb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kcnmb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12365	NM_021452.1 Latest version!	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu44667	XM_006513911.4 Latest version!	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu77590	XM_017314034.2 Latest version!	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu44667	XM_006513911.3	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu77590	XM_017314034.1	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kcnmb4 ( NM_021452.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067427.1 Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), mRNA.

Price & Availability↑

CloneID	OMu12365
Clone ID Related Accession (Same CDS sequence)	NM_021452.1
Accession Version	NM_021452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	calcium-activated potassium channel subunit beta-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF215892.1. On Dec 7, 2005 this sequence version replaced XM_916312.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF215892.1, BC048471.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164137, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGTGCT CCTATATCCC GCCCTGTAAG 
AGAGAAAACC AGAAGAACTC GGAGAGCGTC ATGAACTGGC AGCAGTATTG GAAAGATGAG 
ATCGGTTCCC AGCCATTCAC TTGCTATTTT AATCAGCATC AGAGACCAGA GGACGTTCTC 
CTACAGCGCA CACACGACGA GATTGCTCTC CTGCACTGCT TCCTCTGGCC GGTGGTGGCG 
TTTGTGGTGG GTGTTCTCAT CGTGGTCCTG ACCATCTGTG CCAAGAGCCT GGCGGTCAAG 
GCGGAAGCCA TGAAGAAACG CAAGTTCTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067427.1
CDS	3..635
Translation	MAKLRVSYEYTEAEDKSIRLGLFLIVSGILSLFIFGFCWLSPALQDLQATAANCTVLSVQQIGEVFECTFTCGTDCRGTSQYPCVQVYVNNSESNSRALLHSDQHQLLTNPKCSYIPPCKRENQKNSESVMNWQQYWKDEIGSQPFTCYFNQHQRPEDVLLQRTHDEIALLHCFLWPVVAFVVGVLIVVLTICAKSLAVKAEAMKKRKFS

Target ORF information:

RefSeq Version	NM_021452.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021452.1

ORF Insert Sequence:

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGTGCT CCTATATCCC GCCCTGTAAG 
AGAGAAAACC AGAAGAACTC GGAGAGCGTC ATGAACTGGC AGCAGTATTG GAAAGATGAG 
ATCGGTTCCC AGCCATTCAC TTGCTATTTT AATCAGCATC AGAGACCAGA GGACGTTCTC 
CTACAGCGCA CACACGACGA GATTGCTCTC CTGCACTGCT TCCTCTGGCC GGTGGTGGCG 
TTTGTGGTGG GTGTTCTCAT CGTGGTCCTG ACCATCTGTG CCAAGAGCCT GGCGGTCAAG 
GCGGAAGCCA TGAAGAAACG CAAGTTCTCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnmb4 ( XM_006513911.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513974.1 Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44667
Clone ID Related Accession (Same CDS sequence)	XM_006513911.4 , XM_006513911.3
Accession Version	XM_006513911.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calcium-activated potassium channel subunit beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513911.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGGACC CTGTCACCAG TCCCTTCATG 
TATGGTCTGT TTCATCTTCC AAGCCGCCAT TTTTCGAAGT GTGGCCAGCA TTCAAGCAGC 
ATCAGCGCCA TCTGTGGGCT GGACAGAAAT GCTCATGCTC TGGTCTCAGC CTCGATTCCA 
CCCAAGTCCC ATGGTCAAAT CCAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513974.1
CDS	22..531
Translation	MAKLRVSYEYTEAEDKSIRLGLFLIVSGILSLFIFGFCWLSPALQDLQATAANCTVLSVQQIGEVFECTFTCGTDCRGTSQYPCVQVYVNNSESNSRALLHSDQHQLLTNPKDPVTSPFMYGLFHLPSRHFSKCGQHSSSISAICGLDRNAHALVSASIPPKSHGQIQP

Target ORF information:

RefSeq Version	XM_006513911.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513911.3

ORF Insert Sequence:

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGGACC CTGTCACCAG TCCCTTCATG 
TATGGTCTGT TTCATCTTCC AAGCCGCCAT TTTTCGAAGT GTGGCCAGCA TTCAAGCAGC 
ATCAGCGCCA TCTGTGGGCT GGACAGAAAT GCTCATGCTC TGGTCTCAGC CTCGATTCCA 
CCCAAGTCCC ATGGTCAAAT CCAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnmb4 ( XM_006513911.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513974.1 Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44667
Clone ID Related Accession (Same CDS sequence)	XM_006513911.4 , XM_006513911.3
Accession Version	XM_006513911.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calcium-activated potassium channel subunit beta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513911.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGGACC CTGTCACCAG TCCCTTCATG 
TATGGTCTGT TTCATCTTCC AAGCCGCCAT TTTTCGAAGT GTGGCCAGCA TTCAAGCAGC 
ATCAGCGCCA TCTGTGGGCT GGACAGAAAT GCTCATGCTC TGGTCTCAGC CTCGATTCCA 
CCCAAGTCCC ATGGTCAAAT CCAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513974.1
CDS	23..532
Translation	MAKLRVSYEYTEAEDKSIRLGLFLIVSGILSLFIFGFCWLSPALQDLQATAANCTVLSVQQIGEVFECTFTCGTDCRGTSQYPCVQVYVNNSESNSRALLHSDQHQLLTNPKDPVTSPFMYGLFHLPSRHFSKCGQHSSSISAICGLDRNAHALVSASIPPKSHGQIQP

Target ORF information:

RefSeq Version	XM_006513911.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513911.4

ORF Insert Sequence:

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGGACC CTGTCACCAG TCCCTTCATG 
TATGGTCTGT TTCATCTTCC AAGCCGCCAT TTTTCGAAGT GTGGCCAGCA TTCAAGCAGC 
ATCAGCGCCA TCTGTGGGCT GGACAGAAAT GCTCATGCTC TGGTCTCAGC CTCGATTCCA 
CCCAAGTCCC ATGGTCAAAT CCAACCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnmb4 ( XM_017314034.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169523.1 Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77590
Clone ID Related Accession (Same CDS sequence)	XM_017314034.1 , XM_017314034.2
Accession Version	XM_017314034.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	calcium-activated potassium channel subunit beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGACCA GAGGACGTTC TCCTACAGCG 
CACACACGAC GAGATTGCTC TCCTGCACTG CTTCCTCTGG CCGGTGGTGG CGTTTGTGGT 
GGGTGTTCTC ATCGTGGTCC TGACCATCTG TGCCAAGAGC CTGGCGGTCA AGGCGGAAGC 
CATGAAGAAA CGCAAGTTCT CTTAACGGGG AGGAGGAGGA TTGTGGGAAG CCGAGACCAG 
AGGTTGCCTC GTCGGCACTC GCCTGCCCTG CAGAGCCCAC ATTTGGTGGC GCAGGAGATG 
GAAGGGGCCG CAAGAGGCCC CCGAGAGGCC CCTCCCTCGT CGGCAGCAGA AACAGGCACA 
GTTGGAAGAC TGGGGGGACT TAACTGCCTG TATTCCATGT GCTGTAGCAA CGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169523.1
CDS	22..738
Translation	MAKLRVSYEYTEAEDKSIRLGLFLIVSGILSLFIFGFCWLSPALQDLQATAANCTVLSVQQIGEVFECTFTCGTDCRGTSQYPCVQVYVNNSESNSRALLHSDQHQLLTNPKTRGRSPTAHTRRDCSPALLPLAGGGVCGGCSHRGPDHLCQEPGGQGGSHEETQVLLTGRRRIVGSRDQRLPRRHSPALQSPHLVAQEMEGAARGPREAPPSSAAETGTVGRLGGLNCLYSMCCSNG

Target ORF information:

RefSeq Version	XM_017314034.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314034.1

ORF Insert Sequence:

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGACCA GAGGACGTTC TCCTACAGCG 
CACACACGAC GAGATTGCTC TCCTGCACTG CTTCCTCTGG CCGGTGGTGG CGTTTGTGGT 
GGGTGTTCTC ATCGTGGTCC TGACCATCTG TGCCAAGAGC CTGGCGGTCA AGGCGGAAGC 
CATGAAGAAA CGCAAGTTCT CTTAACGGGG AGGAGGAGGA TTGTGGGAAG CCGAGACCAG 
AGGTTGCCTC GTCGGCACTC GCCTGCCCTG CAGAGCCCAC ATTTGGTGGC GCAGGAGATG 
GAAGGGGCCG CAAGAGGCCC CCGAGAGGCC CCTCCCTCGT CGGCAGCAGA AACAGGCACA 
GTTGGAAGAC TGGGGGGACT TAACTGCCTG TATTCCATGT GCTGTAGCAA CGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kcnmb4 ( XM_017314034.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169523.1 Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77590
Clone ID Related Accession (Same CDS sequence)	XM_017314034.1 , XM_017314034.2
Accession Version	XM_017314034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	calcium-activated potassium channel subunit beta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGACCA GAGGACGTTC TCCTACAGCG 
CACACACGAC GAGATTGCTC TCCTGCACTG CTTCCTCTGG CCGGTGGTGG CGTTTGTGGT 
GGGTGTTCTC ATCGTGGTCC TGACCATCTG TGCCAAGAGC CTGGCGGTCA AGGCGGAAGC 
CATGAAGAAA CGCAAGTTCT CTTAACGGGG AGGAGGAGGA TTGTGGGAAG CCGAGACCAG 
AGGTTGCCTC GTCGGCACTC GCCTGCCCTG CAGAGCCCAC ATTTGGTGGC GCAGGAGATG 
GAAGGGGCCG CAAGAGGCCC CCGAGAGGCC CCTCCCTCGT CGGCAGCAGA AACAGGCACA 
GTTGGAAGAC TGGGGGGACT TAACTGCCTG TATTCCATGT GCTGTAGCAA CGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169523.1
CDS	23..739
Translation	MAKLRVSYEYTEAEDKSIRLGLFLIVSGILSLFIFGFCWLSPALQDLQATAANCTVLSVQQIGEVFECTFTCGTDCRGTSQYPCVQVYVNNSESNSRALLHSDQHQLLTNPKTRGRSPTAHTRRDCSPALLPLAGGGVCGGCSHRGPDHLCQEPGGQGGSHEETQVLLTGRRRIVGSRDQRLPRRHSPALQSPHLVAQEMEGAARGPREAPPSSAAETGTVGRLGGLNCLYSMCCSNG

Target ORF information:

RefSeq Version	XM_017314034.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus potassium large conductance calcium-activated channel, subfamily M, beta member 4 (Kcnmb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017314034.2

ORF Insert Sequence:

ATGGCGAAGC TCAGGGTGTC TTACGAGTAC ACGGAAGCCG AAGACAAGAG CATCCGGCTC 
GGCTTGTTCC TCATCGTCTC CGGCATCCTG TCGCTCTTCA TCTTCGGCTT CTGCTGGCTC 
AGTCCCGCCT TGCAGGATCT GCAAGCCACG GCGGCCAACT GCACCGTGCT GTCGGTGCAG 
CAGATCGGCG AGGTGTTCGA GTGCACCTTC ACCTGTGGCA CCGACTGCAG GGGCACCTCG 
CAGTATCCCT GCGTCCAGGT GTACGTGAAC AACTCCGAGT CCAACTCCAG GGCGCTGCTA 
CACAGCGACC AGCACCAGCT CCTGACCAAC CCCAAGACCA GAGGACGTTC TCCTACAGCG 
CACACACGAC GAGATTGCTC TCCTGCACTG CTTCCTCTGG CCGGTGGTGG CGTTTGTGGT 
GGGTGTTCTC ATCGTGGTCC TGACCATCTG TGCCAAGAGC CTGGCGGTCA AGGCGGAAGC 
CATGAAGAAA CGCAAGTTCT CTTAACGGGG AGGAGGAGGA TTGTGGGAAG CCGAGACCAG 
AGGTTGCCTC GTCGGCACTC GCCTGCCCTG CAGAGCCCAC ATTTGGTGGC GCAGGAGATG 
GAAGGGGCCG CAAGAGGCCC CCGAGAGGCC CCTCCCTCGT CGGCAGCAGA AACAGGCACA 
GTTGGAAGAC TGGGGGGACT TAACTGCCTG TATTCCATGT GCTGTAGCAA CGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tagging of Endogenous BK Channels with a Fluorogen-Activating Peptide Reveals ?4-Mediated Control of Channel Clustering in Cerebellum.
Frontiers in cellular neuroscience11337(2017)
Pratt CP,Kuljis DA,Homanics GE,He J,Kolodieznyi D,Dudem S,Hollywood MA,Barth AL,Bruchez MP

Knockout of the BK ?4-subunit promotes a functional coupling of BK channels and ryanodine receptors that mediate a fAHP-induced increase in excitability.
Journal of neurophysiology116(2)456-65(2016 Aug)
Wang B,Bugay V,Ling L,Chuang HH,Jaffe DB,Brenner R

Cochlear function in mice lacking the BK channel alpha, beta1, or beta4 subunits.
The Journal of biological chemistry282(5)3312-24(2007 Feb)
Pyott SJ,Meredith AL,Fodor AA,V?zquez AE,Yamoah EN,Aldrich RW

BK channel beta4 subunit reduces dentate gyrus excitability and protects against temporal lobe seizures.
Nature neuroscience8(12)1752-9(2005 Dec)
Brenner R,Chen QH,Vilaythong A,Toney GM,Noebels JL,Aldrich RW

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Kcnmb4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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