Cst10 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cst10
Entrez Gene ID	58214
Full Name	cystatin 10 (chondrocytes)
Synonyms	DD72
General protein information	Preferred Names cystatin 10 (chondrocytes) Names cystatin 10 carminerin cystatin-D
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 G3

mRNA and Protein(s)

mRNA	Protein	Name
NM_021405.2	NP_067380.1	cystatin 10 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cst10	NP_067380.1
	CST5	XP_001097898.1
Homo sapiens (human)	CST5	NP_001891.2
Rattus norvegicus (Norway rat)	Cst5	NP_001102431.1

Gene Ontology
Bibliography

Related articles in PubMed

The reproductive tissue specific cystatin subgroup of genes: expression during gonadal development in wildtype and testatin knockout animals.
Frygelius J, Oscarson M, Nordqvist K, Wedell A, T?h?nen V
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation1(6)363-72(2007)

Carminerin contributes to chondrocyte calcification during endochondral ossification.
Yamada T, Kawano H, Koshizuka Y, Fukuda T, Yoshimura K, Kamekura S, Saito T, Ikeda T, Kawasaki Y, Azuma Y, Ikegawa S, Hoshi K, Chung UI, Nakamura K, Kato S, Kawaguchi H
Nature medicine12(6)665-70(2006 Jun)

Cystatin 10, a novel chondrocyte-specific protein, may promote the last steps of the chondrocyte differentiation pathway.
Koshizuka Y, Yamada T, Hoshi K, Ogasawara T, Chung UI, Kawano H, Nakamura Y, Nakamura K, Ikegawa S, Kawaguchi H
The Journal of biological chemistry278(48)48259-66(2003 Nov)

Isolation of novel mouse genes associated with ectopic ossification by differential display method using ttw, a mouse model for ectopic ossification.
Koshizuka Y, Ikegawa S, Sano M, Nakamura K, Nakamura Y
Cytogenetics and cell genetics94(3-4)163-8(2001)

Molecular cloning, chromosome mapping and characterization of a testis-specific cystatin-like cDNA, cystatin T.
Shoemaker K, Holloway JL, Whitmore TE, Maurer M, Feldhaus AL
Gene245(1)103-8(2000 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that cystatin 10 (carminerin) contributes to chondrocyte calcification during endochondral ossification under physiological and pathological conditions through the transcriptional inhibition of NPP1.
Title: Carminerin contributes to chondrocyte calcification during endochondral ossification.

Cst10 may play an important role in the last steps of the chondrocyte differentiation pathway as an inducer of maturation, followed by apoptosis of chondrocytes
Title: Cystatin 10, a novel chondrocyte-specific protein, may promote the last steps of the chondrocyte differentiation pathway.

The following Cst10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cst10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu25350	NM_021405.2 Latest version!	Mus musculus cystatin 10 (chondrocytes) (Cst10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cst10 ( NM_021405.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067380.1 Mus musculus cystatin 10 (chondrocytes) (Cst10), mRNA.

Price & Availability↑

CloneID	OMu25350
Clone ID Related Accession (Same CDS sequence)	NM_021405.2
Accession Version	NM_021405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	cystatin 10 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL845478.6. On Mar 21, 2007 this sequence version replaced NM_021405.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB036743.1, BG869593.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCAAGTT TGCTGAGCCC CTCGATGCCT GTGTTGGCTG CTGTGGCCTT GACACTGACC 
CTGGCTGTGA TCCCTGAAGC CAGTACTAAT GCTGAAGCCA AGCAGGTGGT ATTAGGTGGA 
GTGGAGCCAG CTGACCCTAA GGATAAGGAG GTGCAGAAAG TGGTAAAGTT TGCTGTTAGA 
ACCTACAATG ATATGGACAA TGACCTGTAT CTTAGCAAGC CAATACGACT GATGAGCGCC 
AGCCAGCAGG TTGTGGCTGG GAAGAACTAC TACTTGAAAA TTGAGCTAGG CCGAACCACA 
TGTACCAAAA CTGAGTCTAA TTTGGTTGAT TGTCCCTTCA ATGAACAGCC AGATCAGCAG 
AAGAGGGTCA TCTGTAATTT CCAGATCAAC GTTGCACCTT GGCTGAACAA GATGTCCATG 
ACAAACTTCA ACTGTTACAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067380.1
CDS	118..564
Translation	MASLLSPSMPVLAAVALTLTLAVIPEASTNAEAKQVVLGGVEPADPKDKEVQKVVKFAVRTYNDMDNDLYLSKPIRLMSASQQVVAGKNYYLKIELGRTTCTKTESNLVDCPFNEQPDQQKRVICNFQINVAPWLNKMSMTNFNCYNF

Target ORF information:

RefSeq Version	NM_021405.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cystatin 10 (chondrocytes) (Cst10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021405.2

ORF Insert Sequence:

ATGGCAAGTT TGCTGAGCCC CTCGATGCCT GTGTTGGCTG CTGTGGCCTT GACACTGACC 
CTGGCTGTGA TCCCTGAAGC CAGTACTAAT GCTGAAGCCA AGCAGGTGGT ATTAGGTGGA 
GTGGAGCCAG CTGACCCTAA GGATAAGGAG GTGCAGAAAG TGGTAAAGTT TGCTGTTAGA 
ACCTACAATG ATATGGACAA TGACCTGTAT CTTAGCAAGC CAATACGACT GATGAGCGCC 
AGCCAGCAGG TTGTGGCTGG GAAGAACTAC TACTTGAAAA TTGAGCTAGG CCGAACCACA 
TGTACCAAAA CTGAGTCTAA TTTGGTTGAT TGTCCCTTCA ATGAACAGCC AGATCAGCAG 
AAGAGGGTCA TCTGTAATTT CCAGATCAAC GTTGCACCTT GGCTGAACAA GATGTCCATG 
ACAAACTTCA ACTGTTACAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The reproductive tissue specific cystatin subgroup of genes: expression during gonadal development in wildtype and testatin knockout animals.
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation1(6)363-72(2007)
Frygelius J,Oscarson M,Nordqvist K,Wedell A,T?h?nen V

Carminerin contributes to chondrocyte calcification during endochondral ossification.
Nature medicine12(6)665-70(2006 Jun)
Yamada T,Kawano H,Koshizuka Y,Fukuda T,Yoshimura K,Kamekura S,Saito T,Ikeda T,Kawasaki Y,Azuma Y,Ikegawa S,Hoshi K,Chung UI,Nakamura K,Kato S,Kawaguchi H

Cystatin 10, a novel chondrocyte-specific protein, may promote the last steps of the chondrocyte differentiation pathway.
The Journal of biological chemistry278(48)48259-66(2003 Nov)
Koshizuka Y,Yamada T,Hoshi K,Ogasawara T,Chung UI,Kawano H,Nakamura Y,Nakamura K,Ikegawa S,Kawaguchi H

Isolation of novel mouse genes associated with ectopic ossification by differential display method using ttw, a mouse model for ectopic ossification.
Cytogenetics and cell genetics94(3-4)163-8(2001)
Koshizuka Y,Ikegawa S,Sano M,Nakamura K,Nakamura Y

Molecular cloning, chromosome mapping and characterization of a testis-specific cystatin-like cDNA, cystatin T.
Gene245(1)103-8(2000 Mar)
Shoemaker K,Holloway JL,Whitmore TE,Maurer M,Feldhaus AL