Zfp318 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zfp318
Entrez Gene ID	57908
Full Name	zinc finger protein 318
Synonyms	2610034E08Rik,D530032D06Rik,TZF,Znf318
General protein information	Preferred Names zinc finger protein 318 Names zinc finger protein 318 testicular zinc finger protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_021346.2	NP_067321.2	zinc finger protein 318 isoform 2
XM_011246554.3	XP_011244856.1	zinc finger protein 318 isoform X1
XM_011246554.2	XP_011244856.1	zinc finger protein 318 isoform X1
NM_207671.4	NP_997554.2	zinc finger protein 318 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZNF318	NP_055160.2
Canis lupus familiaris (dog)	ZNF318	XP_851845.2
Bos taurus (cattle)	ZNF318	XP_002697344.2
Mus musculus (house mouse)	Zfp318	NP_997554.2
Rattus norvegicus (Norway rat)	Zfp318	XP_001062211.4
Pan troglodytes (chimpanzee)	ZNF318	XP_518490.3
	ZNF318	XP_002803802.1

Gene Ontology
Bibliography

Related articles in PubMed

Abnormal spermatogenesis and male infertility in testicular zinc finger protein Zfp318-knockout mice.
Ishizuka M, Ohtsuka E, Inoue A, Odaka M, Ohshima H, Tamura N, Yoshida K, Sako N, Baba T, Kashiwabara S, Okabe M, Noguchi J, Hagiwara H
Development, growth & differentiation58(7)600-8(2016 Sep)

Zfp318 regulates IgD expression by abrogating transcription termination within the Ighm/Ighd locus.
Pioli PD, Debnath I, Weis JJ, Weis JH
Journal of immunology (Baltimore, Md. : 1950)193(5)2546-53(2014 Sep)

Zinc-finger protein ZFP318 is essential for expression of IgD, the alternatively spliced Igh product made by mature B lymphocytes.
Enders A, Short A, Miosge LA, Bergmann H, Sontani Y, Bertram EM, Whittle B, Balakishnan B, Yoshida K, Sjollema G, Field MA, Andrews TD, Hagiwara H, Goodnow CC
Proceedings of the National Academy of Sciences of the United States of America111(12)4513-8(2014 Mar)

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

Opposite effects of alternative TZF spliced variants on androgen receptor.
Tao RH, Kawate H, Ohnaka K, Ishizuka M, Hagiwara H, Takayanagi R
Biochemical and biophysical research communications341(2)515-21(2006 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zfp318 is expressed as a functional protein in testicular germ cells and plays an important role in meiosis during spermatogenesis.
Title: Abnormal spermatogenesis and male infertility in testicular zinc finger protein Zfp318-knockout mice.

This finding indicates that Zfp318, in a novel manner, functions by repressing recognition of the transcriptional termination site at the 3' end of the terminal IgM-encoding exon
Title: Zfp318 regulates IgD expression by abrogating transcription termination within the Ighm/Ighd locus.

findings identify ZFP318 as a crucial factor regulating the expression of the two major antibody isotypes on the surface of most mature B cells
Title: Zinc-finger protein ZFP318 is essential for expression of IgD, the alternatively spliced Igh product made by mature B lymphocytes.

in the unique regulation system of AR-mediated transactivation, two spliced isoforms of TZF act as coactivator and corepressor, respectively
Title: Opposite effects of alternative TZF spliced variants on androgen receptor.

Molecular cloning and characteristics of a novel zinc finger protein and its splice variant whose transcripts are expressed during spermatogenesis.
Title: Molecular cloning and characteristics of a novel zinc finger protein and its splice variant whose transcripts are expressed during spermatogenesis.

The following Zfp318 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfp318 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69760	XM_011246554.3 Latest version!	Mus musculus zinc finger protein 318 (Zfp318), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMu10119	NM_207671.4 Latest version!	Mus musculus zinc finger protein 318 (Zfp318), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1644.30 $2349.00
OMu12213	NM_021346.2 Latest version!	Mus musculus zinc finger protein 318 (Zfp318), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$678.30 $969.00
View Old Accession Versions >>
OMu69760	XM_011246554.2	Mus musculus zinc finger protein 318 (Zfp318), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zfp318 ( XM_011246554.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244856.1 Mus musculus zinc finger protein 318 (Zfp318), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69760
Clone ID Related Accession (Same CDS sequence)	XM_011246554.3 , XM_011246554.2
Accession Version	XM_011246554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	zinc finger protein 318 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGG ATGACCTGAC TGATGATTCT GTCTTCACTC GAAGCTCCCA GTGTTCTCGG 
GGTCTTGAAC GATACATTTC TCGGGAGGAG GGACCACTTA GTCCCTTCTT GGGACAACTT 
GATGAGGACT ACCGAACAAG AGAAACTTTT CTGCATCGAC CTGAGTTTAG TCCCCAGAGC 
AGTTGTCATG ATGAATTGTT GAGGGGAACA GAGCGGAATA GAGACAAACT TAAAAGCTCC 
TCCTATTCCA TACGATCTGA GGAAAGAAGC CGGGAGGCCA AAAGGCCCCG TTATGATGAC 
ACAGAGAAGG TACACAGCTC AGGAGGGGAT CACTCAAGTT TTACATCTGG GACTCGAAAC 
TATCGGCAGC GAAGAAGCAG CCCAAGCCCT AGGTTTCTAG ATCCCGAGTT TCGAGAACTG 
GACCTTGCCA GGCGAAAGCG AGAGGAAGAA GAGGAACAAA GTAGGAGCTT GAGTCAAGAG 
CTGGTGGGTG TTGGTGATGA TCAGATTGGC TGTTCCATCC CTGGCTTGGC AGGAGTCCTA 
ACCACATCAG AGCCTGGATA TTCTTTGCAG CGGCCTGAGG AAGTACCTAT GATGCCCAAG 
AAGTCCATTC TGAAGAAACG AATTGAAGCG GACATGAAGC CTTCCTTGCA GCTTGAGAGC 
TTTTCTAGCG GTGCCAGCTC TGGTGAAGAT CACCCTCTTT ATTCTGAACA TTCACCTCTT 
CCTCTAAGTG GTGCTATTGC TGCTTTTACT TCAGAGATTG AAAACAAGGG GACTACAGTG 
GAGGCTGACC TGAAAGAGCC TCAGAGTAAC CTATACCAGT GGGGCCCCCT CCGTGAGATA 
CCTAAAGACA ACAGTGAAAA ATTTGACAGT TTTCTAGGCT TTAAGGAAAA ATTGGATTTG 
AAGGCTGAGG GACTTGAGCA ACAGACAGAT TTCTTACTGC CCCATGAGAG AGCTAGCCAG 
GACGGCAGTG GCTTTTCTCG AATTCTGAGC ATGTTGGCTG ATCCTACCAT TACACAGGAA 
AAAAGAAGAC GTAGCTTCCC TGATATTGAG GATGAGGAGA AATTTCTCTA TGGGGATGAA 
GAAGAGGATA TAAAATCAGA ATCTCCACTT AAGTCCCTGG AGGACCCCGA GAGTGCAGGT 
ACGAGACAGA AGGCAAACTC TCTGCCCTCA ACTCCAGCTG TAAAGCTAGA ATCACTAGAA 
GAAAGCAATC CAGAATATGC CAAGATTCAC AACTTGCTTA AGACCATAGG GCTGGATATT 
GGAGTAGCAG AAATTGGTAA ATTGGCTGCA CGAACCCAGG AGAGACTTCA TGGCAAGAAG 
CCATCATCAC GCCCCTCAGC TGACCGACGT TTGTCAGCTG ACCGACACTT GTCTGGTGAC 
CGACACTTTT CAGCTGACCG CTGTTCCTCA GTTGAGCACA GTTTCACAGC TGATTGGCGC 
TCATCAGACC CTCACAGACC GGAGAGTAGG GAGACACATC ATAGCAATAC TCAGTCCCCA 
GAAGTATCTC ATCCACACCC AGCTTCCCCG GTGGATCCTT ACTTGCGCAC CAAAAATAGC 
CCTCCATTCC TAAAGTCTGA CCATCCAGTG TGCCATGTTT CAGGACCAGA GGTAGTTGGC 
AGTGGGTTTC AATCATCTGT TGCTGTTCGG TGCATGTTGC CATCAGCCCC ATCTACCCCA 
ATTAGACTTC CTCACTCTGC TGCGTTGTCT CAGTTTCATA TACCAGGGGC CTCTCAGTTT 
GCTGCAGCTC GGATACCTCC AAATTATCAG GGATCTGTCA TTCCCTCTGC CTCCTTTGAT 
GCATATAGGC ACTACATGGC ATATGCAGCC TCAAGGTGGC CTATGTATCC TGCCTCTCAA 
CCACCAAGCC ACCCTTTATC TGACCCTCAT AGGCTATTAC CAGTAACCAA ACAAGCTGCT 
CGTAGTCGTC CCAATCTCCG GGTGATCCCT ACTGTGACTC CTGCAAAGCC CAAACAAGAA 
ATACCAGTAC TAGGCTCAAT TTCTGTAAAG CGAATTCCTG TTCGGGTGTC CATTCCATCA 
CTCATAAAAT ATAATCCAAA GAAGATCTCT GATGAGAAGA ACCGTGCCTC CCAGAAGCAG 
AAGGTTATTG AAGAGAGAGA AAAACTGAAA ACTGAACAGG AAGCCCGGCA AAAGAAAATG 
TTTTATCTCA CGACTGAGTT GGAGCGGCTC CATAAACAGC AAGGGGAGAT GTTGCGTAAG 
AAGCGAAGGG AGAAGGATGG TCACAAAGAT CCACTTCTGA TGGAGGTGAG TCGACTTCAG 
GATAGTATTA TGAAGGACAT TGCCGAGCTA CATAAAGAGA CAGAAGAAGC AGAAAAGAAG 
CAGTCTGAAC TGGACAAAGT GGCTCAGATC TTGGGAATTG ATATCTTTGA TAAATCCCTG 
AAATCTTCAA ATGACAGTAA AGAGTCTACA GAGAAGCCTG AAAAAGAAAA ATCTAAGAGC 
CCAGAAAAAG AGTTGTCACC CTCAAACTCT TCCTCTAGCA ACAAGGAATC AAAAATGAAT 
GAAAAGTCCT GTATTAAGAG CCCTAGTTCT ACTGAAAGCC TCCAACCAAC TGTTAAGCAG 
TCTGACCAGC CTGTTGCTGC TTATGAGTAT TATGATGCTG GCAGTCACTG GTGCAAAGAC 
TGCAATACCA CCTGTGGGAC CATGTTTGAC TTCTTCACCC ATATGCACAA TAAGAAGCAC 
ACACAGACAC TGGATCCCTA CAACAGACCT TGGGCTTCAA AGACCCAGAG TGAGGCTAAG 
CAAGACACTG TAAAGCGAAC TGACAAGATA ACTGTTCCTG CAAAAGGCTC TGAGTTTCTG 
ATTCCTGTCA CTGGGTTTTA CTGCCAACTC TGTGAGGAAT TTTTGGGGGA TCCAATTTCT 
GGAGAGCAGC ATGTCAAGGG TCACCAGCAC AACGAAAACT ACAAGAAATA TGTGGAAGAA 
AACCCATTGT ATGAAGAACG AAGAAATCTG GACCGCCAAG CTGGCTTGGC TGTCGTTCTA 
GAGACAGAGC GGCGACGGCA AAATGAGCTA AAACGCAAAC TTAATGAGAA ACCAAAGGAG 
GAGAAGATAG AAAAAAAGGC AAGAATTGTG AGAGAAGTAA AGGAAGATGA CAAAGCCCCT 
GGAGAACTAG AGGAGCAGCT CTCGGAGGAT GGGAGTGCCC CTGAGAAAGG TGAGGTGAAG 
GGGAACGCTA GCCTCAGACC CCAGGTGAAA GAAGAGGTAA AGAAGGAGCC ATCAGTAGCA 
TCAATAGCAG CTTCTTTTGG AAAGTTCAGT TGGAAGAAGC CAGAAAAAGA AGAAGAAAAA 
GGTTCAGTGG TGACCCCAGG GGCCCCAAAG GAAGACACGG TGGAAACCAG TAAGGACAGA 
GATGACGGCA AAGCTGAAGT TGGAAAGGCA AAGCCCATCA AAATCAAGCT TTCTGGAAAA 
ACTGTCATTG CACACACCAG TCCCTGGACA CCTGTCGTTA CGACTTCAAC ACAGACTAAG 
ATCCGACCCA ACTTGCCTAT CCCATCCACA GTACTCCGCA AGTCAGGTTC AGCCACAGTG 
AGCAAGCCTG CTCCTCTTAA TACCTTTCTG TCTATCAAGT CCTCTGGAAC ATCCACCAAG 
CCTCTGCCAG TGGTTAAAGA GTCATCATCA GATCTTCTCT TGCCTCCAGA TATCATCTCC 
AAAGCTTTTG GAGGAGAAGA GGTGGTTCTA AAAGGGTCTC CAGAGGAAAA AGTGGAACTG 
GCTGAAAAGA ATGAGCCATC CCAAGTACCT GAGCAAATGC TGGCTCTACT TCCACCGCCA 
CCACCTCCAC CTCCACCCCC ACCTCCACCT CCACCCCCTC CACCACCCCA GGCTGTACCT 
CAGCTATCTG CCCCATCCCC TGCTCAAGCA AATGTTGTCT TAACTCCAGT TAAATCTAAT 
TCGGTGATAT CTCAGACTTT CAGTCTTGGG TTCCAAGGTC CTAACATTTT GAACCCAGGG 
TTGCCTGTGG CCTTCATGGC CTCAGAACAA CCAACTGTAA TTCCTTCTGA TGAGACAGCT 
CCTGGGGTGA GTGAGAGTGA CTGGGACCAG ACCCTAATCT CTATGTTAGT ACGGCCTCCA 
CCACCCCTTT CCAGTGTATT CAGTGAACAG GCCAAAAAAT TAGAAAAGCG AAATTCGTGC 
TTAGCCACAG CCAATGCTAA GGACCTGTAT GATATATTCT ACAGTAGTGG TGGAAAGGGG 
GCCCATGAGA CTAAGCTGAG TAGTAGTACA TTAGCCAATG GGGAAAGCAG TAGCCTTCCT 
AGAACAGAAA GTTCAGATTT CTCCTCCACT TGTACTTTGA ACAGTAGTAT GTCCTCAGAG 
GACCTGCCTC AATGTAGTGC TTTGGTCACT GCTACTGAAA TCAGTAATCT TGAAAATCCT 
ATTTCAAAAG GTATGGAGTC CACAGGGAAA TGGTCTGTTG TAGATCAGAT AGATCCCAAA 
AGCAGAGACA GCACCTATAG CTTCCTACAG CCTCTGACAA GATTATACCA AAATAAGCCT 
TATGAAATAG TTTCCCCAAA GACAGACACT TTGGTCATGT GGACTTCTGG TTCTTCCCAG 
AATGACACTC ATAAAGATAG ACCTCCAGAG GGGAAAATCA GATTTGACCT GGGAGAGCCA 
GGGCCACCTG GTACAGATTC AACTTCTCAT CTATCAGATA CACACTGTCA AACCAACGGA 
CCTCAGAAGT TGATAGAAAT TAACCTCATA GATAACCAAA ACAAGAACCA AGAGGTATAC 
CAGTCAGAGG GTTGCAGAGA AAGTGAAATG AAGAGAAAGA CTGAATTAAA AGGAAAGGTA 
GCAACTGAAG AAGAAGAAGA AGAAGAAGAA GAAGGTGCCA ACAGCATAGA AGATTCTAAT 
TCAAACCATG GCAACAGAAA TACATGGGAA GGAGAAATAG GACAACCCAA GTTGTCAACA 
GTTGACAAAA AGGGAGAACA GTCTAGCAAA TTGATGACCG GACATGAGAA TACAAGTAAA 
GTAGTAATTG AATTGAGTCC GTCTCTCCCT TCCAAAAGGA CAAAAATAGA TTTATTTCCG 
TCATTGCTCC AGAATCCAAA AAGCATGCCT GAACTACTTC TGCTGTCTCC AGCTGGATCA 
GGTTTGTGTT TAAAGAGACA AGAGATTTGG GAGAGGCCAG AAAAACCAGG ATTGGAAGAT 
GTGGAGCTAC AAGGTACCCG TCCAGAACTA ACTGTGACAA TAGAAAGCAA AGTTTTGGAA 
AACTTTGACA CTACACACTT AGAGGTAGAG GGGTTTGCAT CTCTGAGGAA TCTGGGGGAT 
ATGCATGCCA ATTTCCACAA TAGTCAGACA GAACAGACAC GGAGGTCACC AACTGCCCTG 
TCAGAGAAAA TGTCTGAAGA AATTTCTGTG TCATCTGTCA TGTGTAATCC TTCCAGTTCC 
AGTGACATTG AACCAGTACC ATCTTTTTCT GGATTTCCAT TAGAATCTCC CAAAACTTTG 
GTGCTTAACT TTGAAACAGA GGGTGCACAT AGCTCATCTA ATTCCAGAAA TGGGAGAATC 
ACCTCTAACT CTTTGGAAAC TGGACACCCT GTGGAAAATG TTGGCCACGA CTTAGGAGGT 
GAGAGAACAC ACCAGGCCCT TGACCTCTTA GCTGGAGGAA TGTTGTCTGA GGACGTAAAA 
GAAACTTCGC CACTACAGAA AGATTTACTC AGAATGGAAT CCACCACAGT CAGTCCTTCA 
GGCCTTGGAC CATCTCCTTG CCTTCCAGAC CTTGTTGATT TTGTCACACG GACACCTGGA 
GTTCCAAAAC AAAAACCATG TTCTCCACTT TCTGAGCCAG ATGCCTTTCT CAAGTGTAGT 
TCACTGGAGA TGGGATCACC ACCTCCAGAA ATTCTGAGTG TGTCTGTCTC AGAGGTAGCA 
GTTCCTCAAG TAAGTGAAGA CAATGACAGT GCTTTGAATT TGGTAAAAAC TCCACCCTCA 
GGCTCCCCCT CAAGGGATCA GGTAGTTGGA GGCAATGTGA GCCCTCGGGA AATGCCTGAA 
CAAGAAGCTG CAGTTGATGT CATCCCAGAC CACACAAGGT CCAATGTTTA TAACAGTCAG 
GACTACTTAA ATGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244856.1
CDS	594..6671
Translation	MDRDDLTDDSVFTRSSQCSRGLERYISREEGPLSPFLGQLDEDYRTRETFLHRPEFSPQSSCHDELLRGTERNRDKLKSSSYSIRSEERSREAKRPRYDDTEKVHSSGGDHSSFTSGTRNYRQRRSSPSPRFLDPEFRELDLARRKREEEEEQSRSLSQELVGVGDDQIGCSIPGLAGVLTTSEPGYSLQRPEEVPMMPKKSILKKRIEADMKPSLQLESFSSGASSGEDHPLYSEHSPLPLSGAIAAFTSEIENKGTTVEADLKEPQSNLYQWGPLREIPKDNSEKFDSFLGFKEKLDLKAEGLEQQTDFLLPHERASQDGSGFSRILSMLADPTITQEKRRRSFPDIEDEEKFLYGDEEEDIKSESPLKSLEDPESAGTRQKANSLPSTPAVKLESLEESNPEYAKIHNLLKTIGLDIGVAEIGKLAARTQERLHGKKPSSRPSADRRLSADRHLSGDRHFSADRCSSVEHSFTADWRSSDPHRPESRETHHSNTQSPEVSHPHPASPVDPYLRTKNSPPFLKSDHPVCHVSGPEVVGSGFQSSVAVRCMLPSAPSTPIRLPHSAALSQFHIPGASQFAAARIPPNYQGSVIPSASFDAYRHYMAYAASRWPMYPASQPPSHPLSDPHRLLPVTKQAARSRPNLRVIPTVTPAKPKQEIPVLGSISVKRIPVRVSIPSLIKYNPKKISDEKNRASQKQKVIEEREKLKTEQEARQKKMFYLTTELERLHKQQGEMLRKKRREKDGHKDPLLMEVSRLQDSIMKDIAELHKETEEAEKKQSELDKVAQILGIDIFDKSLKSSNDSKESTEKPEKEKSKSPEKELSPSNSSSSNKESKMNEKSCIKSPSSTESLQPTVKQSDQPVAAYEYYDAGSHWCKDCNTTCGTMFDFFTHMHNKKHTQTLDPYNRPWASKTQSEAKQDTVKRTDKITVPAKGSEFLIPVTGFYCQLCEEFLGDPISGEQHVKGHQHNENYKKYVEENPLYEERRNLDRQAGLAVVLETERRRQNELKRKLNEKPKEEKIEKKARIVREVKEDDKAPGELEEQLSEDGSAPEKGEVKGNASLRPQVKEEVKKEPSVASIAASFGKFSWKKPEKEEEKGSVVTPGAPKEDTVETSKDRDDGKAEVGKAKPIKIKLSGKTVIAHTSPWTPVVTTSTQTKIRPNLPIPSTVLRKSGSATVSKPAPLNTFLSIKSSGTSTKPLPVVKESSSDLLLPPDIISKAFGGEEVVLKGSPEEKVELAEKNEPSQVPEQMLALLPPPPPPPPPPPPPPPPPPPQAVPQLSAPSPAQANVVLTPVKSNSVISQTFSLGFQGPNILNPGLPVAFMASEQPTVIPSDETAPGVSESDWDQTLISMLVRPPPPLSSVFSEQAKKLEKRNSCLATANAKDLYDIFYSSGGKGAHETKLSSSTLANGESSSLPRTESSDFSSTCTLNSSMSSEDLPQCSALVTATEISNLENPISKGMESTGKWSVVDQIDPKSRDSTYSFLQPLTRLYQNKPYEIVSPKTDTLVMWTSGSSQNDTHKDRPPEGKIRFDLGEPGPPGTDSTSHLSDTHCQTNGPQKLIEINLIDNQNKNQEVYQSEGCRESEMKRKTELKGKVATEEEEEEEEEGANSIEDSNSNHGNRNTWEGEIGQPKLSTVDKKGEQSSKLMTGHENTSKVVIELSPSLPSKRTKIDLFPSLLQNPKSMPELLLLSPAGSGLCLKRQEIWERPEKPGLEDVELQGTRPELTVTIESKVLENFDTTHLEVEGFASLRNLGDMHANFHNSQTEQTRRSPTALSEKMSEEISVSSVMCNPSSSSDIEPVPSFSGFPLESPKTLVLNFETEGAHSSSNSRNGRITSNSLETGHPVENVGHDLGGERTHQALDLLAGGMLSEDVKETSPLQKDLLRMESTTVSPSGLGPSPCLPDLVDFVTRTPGVPKQKPCSPLSEPDAFLKCSSLEMGSPPPEILSVSVSEVAVPQVSEDNDSALNLVKTPPSGSPSRDQVVGGNVSPREMPEQEAAVDVIPDHTRSNVYNSQDYLNG

Target ORF information:

RefSeq Version	XM_011246554.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 318 (Zfp318), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246554.3

ORF Insert Sequence:

ATGGACAGGG ATGACCTGAC TGATGATTCT GTCTTCACTC GAAGCTCCCA GTGTTCTCGG 
GGTCTTGAAC GATACATTTC TCGGGAGGAG GGACCACTTA GTCCCTTCTT GGGACAACTT 
GATGAGGACT ACCGAACAAG AGAAACTTTT CTGCATCGAC CTGAGTTTAG TCCCCAGAGC 
AGTTGTCATG ATGAATTGTT GAGGGGAACA GAGCGGAATA GAGACAAACT TAAAAGCTCC 
TCCTATTCCA TACGATCTGA GGAAAGAAGC CGGGAGGCCA AAAGGCCCCG TTATGATGAC 
ACAGAGAAGG TACACAGCTC AGGAGGGGAT CACTCAAGTT TTACATCTGG GACTCGAAAC 
TATCGGCAGC GAAGAAGCAG CCCAAGCCCT AGGTTTCTAG ATCCCGAGTT TCGAGAACTG 
GACCTTGCCA GGCGAAAGCG AGAGGAAGAA GAGGAACAAA GTAGGAGCTT GAGTCAAGAG 
CTGGTGGGTG TTGGTGATGA TCAGATTGGC TGTTCCATCC CTGGCTTGGC AGGAGTCCTA 
ACCACATCAG AGCCTGGATA TTCTTTGCAG CGGCCTGAGG AAGTACCTAT GATGCCCAAG 
AAGTCCATTC TGAAGAAACG AATTGAAGCG GACATGAAGC CTTCCTTGCA GCTTGAGAGC 
TTTTCTAGCG GTGCCAGCTC TGGTGAAGAT CACCCTCTTT ATTCTGAACA TTCACCTCTT 
CCTCTAAGTG GTGCTATTGC TGCTTTTACT TCAGAGATTG AAAACAAGGG GACTACAGTG 
GAGGCTGACC TGAAAGAGCC TCAGAGTAAC CTATACCAGT GGGGCCCCCT CCGTGAGATA 
CCTAAAGACA ACAGTGAAAA ATTTGACAGT TTTCTAGGCT TTAAGGAAAA ATTGGATTTG 
AAGGCTGAGG GACTTGAGCA ACAGACAGAT TTCTTACTGC CCCATGAGAG AGCTAGCCAG 
GACGGCAGTG GCTTTTCTCG AATTCTGAGC ATGTTGGCTG ATCCTACCAT TACACAGGAA 
AAAAGAAGAC GTAGCTTCCC TGATATTGAG GATGAGGAGA AATTTCTCTA TGGGGATGAA 
GAAGAGGATA TAAAATCAGA ATCTCCACTT AAGTCCCTGG AGGACCCCGA GAGTGCAGGT 
ACGAGACAGA AGGCAAACTC TCTGCCCTCA ACTCCAGCTG TAAAGCTAGA ATCACTAGAA 
GAAAGCAATC CAGAATATGC CAAGATTCAC AACTTGCTTA AGACCATAGG GCTGGATATT 
GGAGTAGCAG AAATTGGTAA ATTGGCTGCA CGAACCCAGG AGAGACTTCA TGGCAAGAAG 
CCATCATCAC GCCCCTCAGC TGACCGACGT TTGTCAGCTG ACCGACACTT GTCTGGTGAC 
CGACACTTTT CAGCTGACCG CTGTTCCTCA GTTGAGCACA GTTTCACAGC TGATTGGCGC 
TCATCAGACC CTCACAGACC GGAGAGTAGG GAGACACATC ATAGCAATAC TCAGTCCCCA 
GAAGTATCTC ATCCACACCC AGCTTCCCCG GTGGATCCTT ACTTGCGCAC CAAAAATAGC 
CCTCCATTCC TAAAGTCTGA CCATCCAGTG TGCCATGTTT CAGGACCAGA GGTAGTTGGC 
AGTGGGTTTC AATCATCTGT TGCTGTTCGG TGCATGTTGC CATCAGCCCC ATCTACCCCA 
ATTAGACTTC CTCACTCTGC TGCGTTGTCT CAGTTTCATA TACCAGGGGC CTCTCAGTTT 
GCTGCAGCTC GGATACCTCC AAATTATCAG GGATCTGTCA TTCCCTCTGC CTCCTTTGAT 
GCATATAGGC ACTACATGGC ATATGCAGCC TCAAGGTGGC CTATGTATCC TGCCTCTCAA 
CCACCAAGCC ACCCTTTATC TGACCCTCAT AGGCTATTAC CAGTAACCAA ACAAGCTGCT 
CGTAGTCGTC CCAATCTCCG GGTGATCCCT ACTGTGACTC CTGCAAAGCC CAAACAAGAA 
ATACCAGTAC TAGGCTCAAT TTCTGTAAAG CGAATTCCTG TTCGGGTGTC CATTCCATCA 
CTCATAAAAT ATAATCCAAA GAAGATCTCT GATGAGAAGA ACCGTGCCTC CCAGAAGCAG 
AAGGTTATTG AAGAGAGAGA AAAACTGAAA ACTGAACAGG AAGCCCGGCA AAAGAAAATG 
TTTTATCTCA CGACTGAGTT GGAGCGGCTC CATAAACAGC AAGGGGAGAT GTTGCGTAAG 
AAGCGAAGGG AGAAGGATGG TCACAAAGAT CCACTTCTGA TGGAGGTGAG TCGACTTCAG 
GATAGTATTA TGAAGGACAT TGCCGAGCTA CATAAAGAGA CAGAAGAAGC AGAAAAGAAG 
CAGTCTGAAC TGGACAAAGT GGCTCAGATC TTGGGAATTG ATATCTTTGA TAAATCCCTG 
AAATCTTCAA ATGACAGTAA AGAGTCTACA GAGAAGCCTG AAAAAGAAAA ATCTAAGAGC 
CCAGAAAAAG AGTTGTCACC CTCAAACTCT TCCTCTAGCA ACAAGGAATC AAAAATGAAT 
GAAAAGTCCT GTATTAAGAG CCCTAGTTCT ACTGAAAGCC TCCAACCAAC TGTTAAGCAG 
TCTGACCAGC CTGTTGCTGC TTATGAGTAT TATGATGCTG GCAGTCACTG GTGCAAAGAC 
TGCAATACCA CCTGTGGGAC CATGTTTGAC TTCTTCACCC ATATGCACAA TAAGAAGCAC 
ACACAGACAC TGGATCCCTA CAACAGACCT TGGGCTTCAA AGACCCAGAG TGAGGCTAAG 
CAAGACACTG TAAAGCGAAC TGACAAGATA ACTGTTCCTG CAAAAGGCTC TGAGTTTCTG 
ATTCCTGTCA CTGGGTTTTA CTGCCAACTC TGTGAGGAAT TTTTGGGGGA TCCAATTTCT 
GGAGAGCAGC ATGTCAAGGG TCACCAGCAC AACGAAAACT ACAAGAAATA TGTGGAAGAA 
AACCCATTGT ATGAAGAACG AAGAAATCTG GACCGCCAAG CTGGCTTGGC TGTCGTTCTA 
GAGACAGAGC GGCGACGGCA AAATGAGCTA AAACGCAAAC TTAATGAGAA ACCAAAGGAG 
GAGAAGATAG AAAAAAAGGC AAGAATTGTG AGAGAAGTAA AGGAAGATGA CAAAGCCCCT 
GGAGAACTAG AGGAGCAGCT CTCGGAGGAT GGGAGTGCCC CTGAGAAAGG TGAGGTGAAG 
GGGAACGCTA GCCTCAGACC CCAGGTGAAA GAAGAGGTAA AGAAGGAGCC ATCAGTAGCA 
TCAATAGCAG CTTCTTTTGG AAAGTTCAGT TGGAAGAAGC CAGAAAAAGA AGAAGAAAAA 
GGTTCAGTGG TGACCCCAGG GGCCCCAAAG GAAGACACGG TGGAAACCAG TAAGGACAGA 
GATGACGGCA AAGCTGAAGT TGGAAAGGCA AAGCCCATCA AAATCAAGCT TTCTGGAAAA 
ACTGTCATTG CACACACCAG TCCCTGGACA CCTGTCGTTA CGACTTCAAC ACAGACTAAG 
ATCCGACCCA ACTTGCCTAT CCCATCCACA GTACTCCGCA AGTCAGGTTC AGCCACAGTG 
AGCAAGCCTG CTCCTCTTAA TACCTTTCTG TCTATCAAGT CCTCTGGAAC ATCCACCAAG 
CCTCTGCCAG TGGTTAAAGA GTCATCATCA GATCTTCTCT TGCCTCCAGA TATCATCTCC 
AAAGCTTTTG GAGGAGAAGA GGTGGTTCTA AAAGGGTCTC CAGAGGAAAA AGTGGAACTG 
GCTGAAAAGA ATGAGCCATC CCAAGTACCT GAGCAAATGC TGGCTCTACT TCCACCGCCA 
CCACCTCCAC CTCCACCCCC ACCTCCACCT CCACCCCCTC CACCACCCCA GGCTGTACCT 
CAGCTATCTG CCCCATCCCC TGCTCAAGCA AATGTTGTCT TAACTCCAGT TAAATCTAAT 
TCGGTGATAT CTCAGACTTT CAGTCTTGGG TTCCAAGGTC CTAACATTTT GAACCCAGGG 
TTGCCTGTGG CCTTCATGGC CTCAGAACAA CCAACTGTAA TTCCTTCTGA TGAGACAGCT 
CCTGGGGTGA GTGAGAGTGA CTGGGACCAG ACCCTAATCT CTATGTTAGT ACGGCCTCCA 
CCACCCCTTT CCAGTGTATT CAGTGAACAG GCCAAAAAAT TAGAAAAGCG AAATTCGTGC 
TTAGCCACAG CCAATGCTAA GGACCTGTAT GATATATTCT ACAGTAGTGG TGGAAAGGGG 
GCCCATGAGA CTAAGCTGAG TAGTAGTACA TTAGCCAATG GGGAAAGCAG TAGCCTTCCT 
AGAACAGAAA GTTCAGATTT CTCCTCCACT TGTACTTTGA ACAGTAGTAT GTCCTCAGAG 
GACCTGCCTC AATGTAGTGC TTTGGTCACT GCTACTGAAA TCAGTAATCT TGAAAATCCT 
ATTTCAAAAG GTATGGAGTC CACAGGGAAA TGGTCTGTTG TAGATCAGAT AGATCCCAAA 
AGCAGAGACA GCACCTATAG CTTCCTACAG CCTCTGACAA GATTATACCA AAATAAGCCT 
TATGAAATAG TTTCCCCAAA GACAGACACT TTGGTCATGT GGACTTCTGG TTCTTCCCAG 
AATGACACTC ATAAAGATAG ACCTCCAGAG GGGAAAATCA GATTTGACCT GGGAGAGCCA 
GGGCCACCTG GTACAGATTC AACTTCTCAT CTATCAGATA CACACTGTCA AACCAACGGA 
CCTCAGAAGT TGATAGAAAT TAACCTCATA GATAACCAAA ACAAGAACCA AGAGGTATAC 
CAGTCAGAGG GTTGCAGAGA AAGTGAAATG AAGAGAAAGA CTGAATTAAA AGGAAAGGTA 
GCAACTGAAG AAGAAGAAGA AGAAGAAGAA GAAGGTGCCA ACAGCATAGA AGATTCTAAT 
TCAAACCATG GCAACAGAAA TACATGGGAA GGAGAAATAG GACAACCCAA GTTGTCAACA 
GTTGACAAAA AGGGAGAACA GTCTAGCAAA TTGATGACCG GACATGAGAA TACAAGTAAA 
GTAGTAATTG AATTGAGTCC GTCTCTCCCT TCCAAAAGGA CAAAAATAGA TTTATTTCCG 
TCATTGCTCC AGAATCCAAA AAGCATGCCT GAACTACTTC TGCTGTCTCC AGCTGGATCA 
GGTTTGTGTT TAAAGAGACA AGAGATTTGG GAGAGGCCAG AAAAACCAGG ATTGGAAGAT 
GTGGAGCTAC AAGGTACCCG TCCAGAACTA ACTGTGACAA TAGAAAGCAA AGTTTTGGAA 
AACTTTGACA CTACACACTT AGAGGTAGAG GGGTTTGCAT CTCTGAGGAA TCTGGGGGAT 
ATGCATGCCA ATTTCCACAA TAGTCAGACA GAACAGACAC GGAGGTCACC AACTGCCCTG 
TCAGAGAAAA TGTCTGAAGA AATTTCTGTG TCATCTGTCA TGTGTAATCC TTCCAGTTCC 
AGTGACATTG AACCAGTACC ATCTTTTTCT GGATTTCCAT TAGAATCTCC CAAAACTTTG 
GTGCTTAACT TTGAAACAGA GGGTGCACAT AGCTCATCTA ATTCCAGAAA TGGGAGAATC 
ACCTCTAACT CTTTGGAAAC TGGACACCCT GTGGAAAATG TTGGCCACGA CTTAGGAGGT 
GAGAGAACAC ACCAGGCCCT TGACCTCTTA GCTGGAGGAA TGTTGTCTGA GGACGTAAAA 
GAAACTTCGC CACTACAGAA AGATTTACTC AGAATGGAAT CCACCACAGT CAGTCCTTCA 
GGCCTTGGAC CATCTCCTTG CCTTCCAGAC CTTGTTGATT TTGTCACACG GACACCTGGA 
GTTCCAAAAC AAAAACCATG TTCTCCACTT TCTGAGCCAG ATGCCTTTCT CAAGTGTAGT 
TCACTGGAGA TGGGATCACC ACCTCCAGAA ATTCTGAGTG TGTCTGTCTC AGAGGTAGCA 
GTTCCTCAAG TAAGTGAAGA CAATGACAGT GCTTTGAATT TGGTAAAAAC TCCACCCTCA 
GGCTCCCCCT CAAGGGATCA GGTAGTTGGA GGCAATGTGA GCCCTCGGGA AATGCCTGAA 
CAAGAAGCTG CAGTTGATGT CATCCCAGAC CACACAAGGT CCAATGTTTA TAACAGTCAG 
GACTACTTAA ATGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp318 ( NM_207671.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_997554.2 Mus musculus zinc finger protein 318 (Zfp318), transcript variant 1, mRNA.

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CloneID	OMu10119
Clone ID Related Accession (Same CDS sequence)	NM_207671.4
Accession Version	NM_207671.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	zinc finger protein 318 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ161473.1, AW457229.1, CD542244.1, CT009572.21, AK157779.1, AC151275.3 and BU704885.1. On Nov 13, 2013 this sequence version replaced NM_207671.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACCGCA GTGGCTCCCG CTCCTCCGTC TCTTCGCACC GGTCGAAAGA CGGCAGCGCG 
AGCGGCCCTC CGCCGGGCCG TCCCGTGGGC GCCTCCTCAG GCCCCACACG CCGCCCCTCG 
TCGCCGCCGC CGCCCTCTTG CTCCTCGCTG CGGCTCCCCG CCCGCCGCCA CCGCTCGCCC 
TCAGGCCACC GCGGCCGCTG GGCCTCGCCG TCCCCGCCTC GGGGTCGTCG CGGCTCCCCG 
TCCCCGCCCC GCGGCCGCCG GGCCTCACCG TCTCCGACCC GCGGCCGCCG GGCCTCGCCA 
TCCCCTCCGC GGGGTCGTCG TGGCTCCCCG TCCCCGCCGC GCGCCCGTCG CGGCTCCCCG 
TCGCCACCGC GGAGCCGGCG ACACTACCCA CCTGGCCTGG GTGGCTTCCG AGGCAGCATT 
CGAGGCGAGT CCCGCGCCGA CTTCGCCCGG GACGGCCGCG GAGACCATCC AGGCGGCGGC 
GGCGGCAGTC GGAGACGTTC TCCTGGTCTG TGTTCTGATT CTTCTTTGGA GGAGAGTTTA 
AGGATCACAG TTGGCAATGA CCACTTCTGT GTTAGCACAC CAGAGCGGCG GCGGCTCAGT 
GACCGGCTGG GATCTCCAGT GGATGGTCTG CAGGACATGG ACAGGGATGA CCTGACTGAT 
GATTCTGTCT TCACTCGAAG CTCCCAGTGT TCTCGGGGTC TTGAACGATA CATTTCTCGG 
GAGGAGGGAC CACTTAGTCC CTTCTTGGGA CAACTTGATG AGGACTACCG AACAAGAGAA 
ACTTTTCTGC ATCGACCTGA GTTTAGTCCC CAGAGCAGTT GTCATGATGA ATTGTTGAGG 
GGAACAGAGC GGAATAGAGA CAAACTTAAA AGCTCCTCCT ATTCCATACG ATCTGAGGAA 
AGAAGCCGGG AGGCCAAAAG GCCCCGTTAT GATGACACAG AGAAGGTACA CAGCTCAGGA 
GGGGATCACT CAAGTTTTAC ATCTGGGACT CGAAACTATC GGCAGCGAAG AAGCAGCCCA 
AGCCCTAGGT TTCTAGATCC CGAGTTTCGA GAACTGGACC TTGCCAGGCG AAAGCGAGAG 
GAAGAAGAGG AACAAAGTAG GAGCTTGAGT CAAGAGCTGG TGGGTGTTGG TGATGATCAG 
ATTGGCTGTT CCATCCCTGG CTTGGCAGGA GTCCTAACCA CATCAGAGCC TGGATATTCT 
TTGCAGCGGC CTGAGGAAGT ACCTATGATG CCCAAGAAGT CCATTCTGAA GAAACGAATT 
GAAGCGGACA TGAAGCCTTC CTTGCAGCTT GAGAGCTTTT CTAGCGGTGC CAGCTCTGGT 
GAAGATCACC CTCTTTATTC TGAACATTCA CCTCTTCCTC TAAGTGGTGC TATTGCTGCT 
TTTACTTCAG AGATTGAAAA CAAGGGGACT ACAGTGGAGG CTGACCTGAA AGAGCCTCAG 
AGTAACCTAT ACCAGTGGGG CCCCCTCCGT GAGATACCTA AAGACAACAG TGAAAAATTT 
GACAGTTTTC TAGGCTTTAA GGAAAAATTG GATTTGAAGG CTGAGGGACT TGAGCAACAG 
ACAGATTTCT TACTGCCCCA TGAGAGAGCT AGCCAGGACG GCAGTGGCTT TTCTCGAATT 
CTGAGCATGT TGGCTGATCC TACCATTACA CAGGAAAAAA GAAGACGTAG CTTCCCTGAT 
ATTGAGGATG AGGAGAAATT TCTCTATGGG GATGAAGAAG AGGATATAAA ATCAGAATCT 
CCACTTAAGT CCCTGGAGGA CCCCGAGAGT GCAGGTACGA GACAGAAGGC AAACTCTCTG 
CCCTCAACTC CAGCTGTAAA GCTAGAATCA CTAGAAGAAA GCAATCCAGA ATATGCCAAG 
ATTCACAACT TGCTTAAGAC CATAGGGCTG GATATTGGAG TAGCAGAAAT TGGTAAATTG 
GCTGCACGAA CCCAGGAGAG ACTTCATGGC AAGAAGCCAT CATCACGCCC CTCAGCTGAC 
CGACGTTTGT CAGCTGACCG ACACTTGTCT GGTGACCGAC ACTTTTCAGC TGACCGCTGT 
TCCTCAGTTG AGCACAGTTT CACAGCTGAT TGGCGCTCAT CAGACCCTCA CAGACCGGAG 
AGTAGGGAGA CACATCATAG CAATACTCAG TCCCCAGAAG TATCTCATCC ACACCCAGCT 
TCCCCGGTGG ATCCTTACTT GCGCACCAAA AATAGCCCTC CATTCCTAAA GTCTGACCAT 
CCAGTGTGCC ATGTTTCAGG ACCAGAGGTA GTTGGCAGTG GGTTTCAATC ATCTGTTGCT 
GTTCGGTGCA TGTTGCCATC AGCCCCATCT ACCCCAATTA GACTTCCTCA CTCTGCTGCG 
TTGTCTCAGT TTCATATACC AGGGGCCTCT CAGTTTGCTG CAGCTCGGAT ACCTCCAAAT 
TATCAGGGAT CTGTCATTCC CTCTGCCTCC TTTGATGCAT ATAGGCACTA CATGGCATAT 
GCAGCCTCAA GGTGGCCTAT GTATCCTGCC TCTCAACCAC CAAGCCACCC TTTATCTGAC 
CCTCATAGGC TATTACCAGT AACCAAACAA GCTGCTCGTA GTCGTCCCAA TCTCCGGGTG 
ATCCCTACTG TGACTCCTGC AAAGCCCAAA CAAGAAATAC CAGTACTAGG CTCAATTTCT 
GTAAAGCGAA TTCCTGTTCG GGTGTCCATT CCATCACTCA TAAAATATAA TCCAAAGAAG 
ATCTCTGATG AGAAGAACCG TGCCTCCCAG AAGCAGAAGG TTATTGAAGA GAGAGAAAAA 
CTGAAAACTG AACAGGAAGC CCGGCAAAAG AAAATGTTTT ATCTCACGAC TGAGTTGGAG 
CGGCTCCATA AACAGCAAGG GGAGATGTTG CGTAAGAAGC GAAGGGAGAA GGATGGTCAC 
AAAGATCCAC TTCTGATGGA GGTGAGTCGA CTTCAGGATA GTATTATGAA GGACATTGCC 
GAGCTACATA AAGAGACAGA AGAAGCAGAA AAGAAGCAGT CTGAACTGGA CAAAGTGGCT 
CAGATCTTGG GAATTGATAT CTTTGATAAA TCCCTGAAAT CTTCAAATGA CAGTAAAGAG 
TCTACAGAGA AGCCTGAAAA AGAAAAATCT AAGAGCCCAG AAAAAGAGTT GTCACCCTCA 
AACTCTTCCT CTAGCAACAA GGAATCAAAA ATGAATGAAA AGTCCTGTAT TAAGAGCCCT 
AGTTCTACTG AAAGCCTCCA ACCAACTGTT AAGCAGTCTG ACCAGCCTGT TGCTGCTTAT 
GAGTATTATG ATGCTGGCAG TCACTGGTGC AAAGACTGCA ATACCACCTG TGGGACCATG 
TTTGACTTCT TCACCCATAT GCACAATAAG AAGCACACAC AGACACTGGA TCCCTACAAC 
AGACCTTGGG CTTCAAAGAC CCAGAGTGAG GCTAAGCAAG ACACTGTAAA GCGAACTGAC 
AAGATAACTG TTCCTGCAAA AGGCTCTGAG TTTCTGATTC CTGTCACTGG GTTTTACTGC 
CAACTCTGTG AGGAATTTTT GGGGGATCCA ATTTCTGGAG AGCAGCATGT CAAGGGTCAC 
CAGCACAACG AAAACTACAA GAAATATGTG GAAGAAAACC CATTGTATGA AGAACGAAGA 
AATCTGGACC GCCAAGCTGG CTTGGCTGTC GTTCTAGAGA CAGAGCGGCG ACGGCAAAAT 
GAGCTAAAAC GCAAACTTAA TGAGAAACCA AAGGAGGAGA AGATAGAAAA AAAGGCAAGA 
ATTGTGAGAG AAGTAAAGGA AGATGACAAA GCCCCTGGAG AACTAGAGGA GCAGCTCTCG 
GAGGATGGGA GTGCCCCTGA GAAAGGTGAG GTGAAGGGGA ACGCTAGCCT CAGACCCCAG 
GTGAAAGAAG AGGTAAAGAA GGAGCCATCA GTAGCATCAA TAGCAGCTTC TTTTGGAAAG 
TTCAGTTGGA AGAAGCCAGA AAAAGAAGAA GAAAAAGGTT CAGTGGTGAC CCCAGGGGCC 
CCAAAGGAAG ACACGGTGGA AACCAGTAAG GACAGAGATG ACGGCAAAGC TGAAGTTGGA 
AAGGCAAAGC CCATCAAAAT CAAGCTTTCT GGAAAAACTG TCATTGCACA CACCAGTCCC 
TGGACACCTG TCGTTACGAC TTCAACACAG ACTAAGATCC GACCCAACTT GCCTATCCCA 
TCCACAGTAC TCCGCAAGTC AGGTTCAGCC ACAGTGAGCA AGCCTGCTCC TCTTAATACC 
TTTCTGTCTA TCAAGTCCTC TGGAACATCC ACCAAGCCTC TGCCAGTGGT TAAAGAGTCA 
TCATCAGATC TTCTCTTGCC TCCAGATATC ATCTCCAAAG CTTTTGGAGG AGAAGAGGTG 
GTTCTAAAAG GGTCTCCAGA GGAAAAAGTG GAACTGGCTG AAAAGAATGA GCCATCCCAA 
GTACCTGAGC AAATGCTGGC TCTACTTCCA CCGCCACCAC CTCCACCTCC ACCCCCACCT 
CCACCTCCAC CCCCTCCACC ACCCCAGGCT GTACCTCAGC TATCTGCCCC ATCCCCTGCT 
CAAGCAAATG TTGTCTTAAC TCCAGTTAAA TCTAATTCGG TGATATCTCA GACTTTCAGT 
CTTGGGTTCC AAGGTCCTAA CATTTTGAAC CCAGGGTTGC CTGTGGCCTT CATGGCCTCA 
GAACAACCAA CTGTAATTCC TTCTGATGAG ACAGCTCCTG GGGTGAGTGA GAGTGACTGG 
GACCAGACCC TAATCTCTAT GTTAGTACGG CCTCCACCAC CCCTTTCCAG TGTATTCAGT 
GAACAGGCCA AAAAATTAGA AAAGCGAAAT TCGTGCTTAG CCACAGCCAA TGCTAAGGAC 
CTGTATGATA TATTCTACAG TAGTGGTGGA AAGGGGGCCC ATGAGACTAA GCTGAGTAGT 
AGTACATTAG CCAATGGGGA AAGCAGTAGC CTTCCTAGAA CAGAAAGTTC AGATTTCTCC 
TCCACTTGTA CTTTGAACAG TAGTATGTCC TCAGAGGACC TGCCTCAATG TAGTGCTTTG 
GTCACTGCTA CTGAAATCAG TAATCTTGAA AATCCTATTT CAAAAGGTAT GGAGTCCACA 
GGGAAATGGT CTGTTGTAGA TCAGATAGAT CCCAAAAGCA GAGACAGCAC CTATAGCTTC 
CTACAGCCTC TGACAAGATT ATACCAAAAT AAGCCTTATG AAATAGTTTC CCCAAAGACA 
GACACTTTGG TCATGTGGAC TTCTGGTTCT TCCCAGAATG ACACTCATAA AGATAGACCT 
CCAGAGGGGA AAATCAGATT TGACCTGGGA GAGCCAGGGC CACCTGGTAC AGATTCAACT 
TCTCATCTAT CAGATACACA CTGTCAAACC AACGGACCTC AGAAGTTGAT AGAAATTAAC 
CTCATAGATA ACCAAAACAA GAACCAAGAG GTATACCAGT CAGAGGGTTG CAGAGAAAGT 
GAAATGAAGA GAAAGACTGA ATTAAAAGGA AAGGTAGCAA CTGAAGAAGA AGAAGAAGAA 
GAAGAAGAAG GTGCCAACAG CATAGAAGAT TCTAATTCAA ACCATGGCAA CAGAAATACA 
TGGGAAGGAG AAATAGGACA ACCCAAGTTG TCAACAGTTG ACAAAAAGGG AGAACAGTCT 
AGCAAATTGA TGACCGGACA TGAGAATACA AGTAAAGTAG TAATTGAATT GAGTCCGTCT 
CTCCCTTCCA AAAGGACAAA AATAGATTTA TTTCCGTCAT TGCTCCAGAA TCCAAAAAGC 
ATGCCTGAAC TACTTCTGCT GTCTCCAGCT GGATCAGGTT TGTGTTTAAA GAGACAAGAG 
ATTTGGGAGA GGCCAGAAAA ACCAGGATTG GAAGATGTGG AGCTACAAGG TACCCGTCCA 
GAACTAACTG TGACAATAGA AAGCAAAGTT TTGGAAAACT TTGACACTAC ACACTTAGAG 
GTAGAGGGGT TTGCATCTCT GAGGAATCTG GGGGATATGC ATGCCAATTT CCACAATAGT 
CAGACAGAAC AGACACGGAG GTCACCAACT GCCCTGTCAG AGAAAATGTC TGAAGAAATT 
TCTGTGTCAT CTGTCATGTG TAATCCTTCC AGTTCCAGTG ACATTGAACC AGTACCATCT 
TTTTCTGGAT TTCCATTAGA ATCTCCCAAA ACTTTGGTGC TTAACTTTGA AACAGAGGGT 
GCACATAGCT CATCTAATTC CAGAAATGGG AGAATCACCT CTAACTCTTT GGAAACTGGA 
CACCCTGTGG AAAATGTTGG CCACGACTTA GGAGGTGAGA GAACACACCA GGCCCTTGAC 
CTCTTAGCTG GAGGAATGTT GTCTGAGGAC GTAAAAGAAA CTTCGCCACT ACAGAAAGAT 
TTACTCAGAA TGGAATCCAC CACAGTCAGT CCTTCAGGCC TTGGACCATC TCCTTGCCTT 
CCAGACCTTG TTGATTTTGT CACACGGACA CCTGGAGTTC CAAAACAAAA ACCATGTTCT 
CCACTTTCTG AGCCAGATGC CTTTCTCAAG TGTAGTTCAC TGGAGATGGG ATCACCACCT 
CCAGAAATTC TGAGTGTGTC TGTCTCAGAG GTAGCAGTTC CTCAAGTAAG TGAAGACAAT 
GACAGTGCTT TGAATTTGGT AAAAACTCCA CCCTCAGGCT CCCCCTCAAG GGATCAGGTA 
GTTGGAGGCA ATGTGAGCCC TCGGGAAATG CCTGAACAAG AAGCTGCAGT TGATGTCATC 
CCAGACCACA CAAGGTCCAA TGTTTATAAC AGTCAGGACT ACTTAAATGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997554.2
CDS	74..6787
Translation	MYRSGSRSSVSSHRSKDGSASGPPPGRPVGASSGPTRRPSSPPPPSCSSLRLPARRHRSPSGHRGRWASPSPPRGRRGSPSPPRGRRASPSPTRGRRASPSPPRGRRGSPSPPRARRGSPSPPRSRRHYPPGLGGFRGSIRGESRADFARDGRGDHPGGGGGSRRRSPGLCSDSSLEESLRITVGNDHFCVSTPERRRLSDRLGSPVDGLQDMDRDDLTDDSVFTRSSQCSRGLERYISREEGPLSPFLGQLDEDYRTRETFLHRPEFSPQSSCHDELLRGTERNRDKLKSSSYSIRSEERSREAKRPRYDDTEKVHSSGGDHSSFTSGTRNYRQRRSSPSPRFLDPEFRELDLARRKREEEEEQSRSLSQELVGVGDDQIGCSIPGLAGVLTTSEPGYSLQRPEEVPMMPKKSILKKRIEADMKPSLQLESFSSGASSGEDHPLYSEHSPLPLSGAIAAFTSEIENKGTTVEADLKEPQSNLYQWGPLREIPKDNSEKFDSFLGFKEKLDLKAEGLEQQTDFLLPHERASQDGSGFSRILSMLADPTITQEKRRRSFPDIEDEEKFLYGDEEEDIKSESPLKSLEDPESAGTRQKANSLPSTPAVKLESLEESNPEYAKIHNLLKTIGLDIGVAEIGKLAARTQERLHGKKPSSRPSADRRLSADRHLSGDRHFSADRCSSVEHSFTADWRSSDPHRPESRETHHSNTQSPEVSHPHPASPVDPYLRTKNSPPFLKSDHPVCHVSGPEVVGSGFQSSVAVRCMLPSAPSTPIRLPHSAALSQFHIPGASQFAAARIPPNYQGSVIPSASFDAYRHYMAYAASRWPMYPASQPPSHPLSDPHRLLPVTKQAARSRPNLRVIPTVTPAKPKQEIPVLGSISVKRIPVRVSIPSLIKYNPKKISDEKNRASQKQKVIEEREKLKTEQEARQKKMFYLTTELERLHKQQGEMLRKKRREKDGHKDPLLMEVSRLQDSIMKDIAELHKETEEAEKKQSELDKVAQILGIDIFDKSLKSSNDSKESTEKPEKEKSKSPEKELSPSNSSSSNKESKMNEKSCIKSPSSTESLQPTVKQSDQPVAAYEYYDAGSHWCKDCNTTCGTMFDFFTHMHNKKHTQTLDPYNRPWASKTQSEAKQDTVKRTDKITVPAKGSEFLIPVTGFYCQLCEEFLGDPISGEQHVKGHQHNENYKKYVEENPLYEERRNLDRQAGLAVVLETERRRQNELKRKLNEKPKEEKIEKKARIVREVKEDDKAPGELEEQLSEDGSAPEKGEVKGNASLRPQVKEEVKKEPSVASIAASFGKFSWKKPEKEEEKGSVVTPGAPKEDTVETSKDRDDGKAEVGKAKPIKIKLSGKTVIAHTSPWTPVVTTSTQTKIRPNLPIPSTVLRKSGSATVSKPAPLNTFLSIKSSGTSTKPLPVVKESSSDLLLPPDIISKAFGGEEVVLKGSPEEKVELAEKNEPSQVPEQMLALLPPPPPPPPPPPPPPPPPPPQAVPQLSAPSPAQANVVLTPVKSNSVISQTFSLGFQGPNILNPGLPVAFMASEQPTVIPSDETAPGVSESDWDQTLISMLVRPPPPLSSVFSEQAKKLEKRNSCLATANAKDLYDIFYSSGGKGAHETKLSSSTLANGESSSLPRTESSDFSSTCTLNSSMSSEDLPQCSALVTATEISNLENPISKGMESTGKWSVVDQIDPKSRDSTYSFLQPLTRLYQNKPYEIVSPKTDTLVMWTSGSSQNDTHKDRPPEGKIRFDLGEPGPPGTDSTSHLSDTHCQTNGPQKLIEINLIDNQNKNQEVYQSEGCRESEMKRKTELKGKVATEEEEEEEEEGANSIEDSNSNHGNRNTWEGEIGQPKLSTVDKKGEQSSKLMTGHENTSKVVIELSPSLPSKRTKIDLFPSLLQNPKSMPELLLLSPAGSGLCLKRQEIWERPEKPGLEDVELQGTRPELTVTIESKVLENFDTTHLEVEGFASLRNLGDMHANFHNSQTEQTRRSPTALSEKMSEEISVSSVMCNPSSSSDIEPVPSFSGFPLESPKTLVLNFETEGAHSSSNSRNGRITSNSLETGHPVENVGHDLGGERTHQALDLLAGGMLSEDVKETSPLQKDLLRMESTTVSPSGLGPSPCLPDLVDFVTRTPGVPKQKPCSPLSEPDAFLKCSSLEMGSPPPEILSVSVSEVAVPQVSEDNDSALNLVKTPPSGSPSRDQVVGGNVSPREMPEQEAAVDVIPDHTRSNVYNSQDYLNG

Target ORF information:

RefSeq Version	NM_207671.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 318 (Zfp318), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207671.4

ORF Insert Sequence:

ATGTACCGCA GTGGCTCCCG CTCCTCCGTC TCTTCGCACC GGTCGAAAGA CGGCAGCGCG 
AGCGGCCCTC CGCCGGGCCG TCCCGTGGGC GCCTCCTCAG GCCCCACACG CCGCCCCTCG 
TCGCCGCCGC CGCCCTCTTG CTCCTCGCTG CGGCTCCCCG CCCGCCGCCA CCGCTCGCCC 
TCAGGCCACC GCGGCCGCTG GGCCTCGCCG TCCCCGCCTC GGGGTCGTCG CGGCTCCCCG 
TCCCCGCCCC GCGGCCGCCG GGCCTCACCG TCTCCGACCC GCGGCCGCCG GGCCTCGCCA 
TCCCCTCCGC GGGGTCGTCG TGGCTCCCCG TCCCCGCCGC GCGCCCGTCG CGGCTCCCCG 
TCGCCACCGC GGAGCCGGCG ACACTACCCA CCTGGCCTGG GTGGCTTCCG AGGCAGCATT 
CGAGGCGAGT CCCGCGCCGA CTTCGCCCGG GACGGCCGCG GAGACCATCC AGGCGGCGGC 
GGCGGCAGTC GGAGACGTTC TCCTGGTCTG TGTTCTGATT CTTCTTTGGA GGAGAGTTTA 
AGGATCACAG TTGGCAATGA CCACTTCTGT GTTAGCACAC CAGAGCGGCG GCGGCTCAGT 
GACCGGCTGG GATCTCCAGT GGATGGTCTG CAGGACATGG ACAGGGATGA CCTGACTGAT 
GATTCTGTCT TCACTCGAAG CTCCCAGTGT TCTCGGGGTC TTGAACGATA CATTTCTCGG 
GAGGAGGGAC CACTTAGTCC CTTCTTGGGA CAACTTGATG AGGACTACCG AACAAGAGAA 
ACTTTTCTGC ATCGACCTGA GTTTAGTCCC CAGAGCAGTT GTCATGATGA ATTGTTGAGG 
GGAACAGAGC GGAATAGAGA CAAACTTAAA AGCTCCTCCT ATTCCATACG ATCTGAGGAA 
AGAAGCCGGG AGGCCAAAAG GCCCCGTTAT GATGACACAG AGAAGGTACA CAGCTCAGGA 
GGGGATCACT CAAGTTTTAC ATCTGGGACT CGAAACTATC GGCAGCGAAG AAGCAGCCCA 
AGCCCTAGGT TTCTAGATCC CGAGTTTCGA GAACTGGACC TTGCCAGGCG AAAGCGAGAG 
GAAGAAGAGG AACAAAGTAG GAGCTTGAGT CAAGAGCTGG TGGGTGTTGG TGATGATCAG 
ATTGGCTGTT CCATCCCTGG CTTGGCAGGA GTCCTAACCA CATCAGAGCC TGGATATTCT 
TTGCAGCGGC CTGAGGAAGT ACCTATGATG CCCAAGAAGT CCATTCTGAA GAAACGAATT 
GAAGCGGACA TGAAGCCTTC CTTGCAGCTT GAGAGCTTTT CTAGCGGTGC CAGCTCTGGT 
GAAGATCACC CTCTTTATTC TGAACATTCA CCTCTTCCTC TAAGTGGTGC TATTGCTGCT 
TTTACTTCAG AGATTGAAAA CAAGGGGACT ACAGTGGAGG CTGACCTGAA AGAGCCTCAG 
AGTAACCTAT ACCAGTGGGG CCCCCTCCGT GAGATACCTA AAGACAACAG TGAAAAATTT 
GACAGTTTTC TAGGCTTTAA GGAAAAATTG GATTTGAAGG CTGAGGGACT TGAGCAACAG 
ACAGATTTCT TACTGCCCCA TGAGAGAGCT AGCCAGGACG GCAGTGGCTT TTCTCGAATT 
CTGAGCATGT TGGCTGATCC TACCATTACA CAGGAAAAAA GAAGACGTAG CTTCCCTGAT 
ATTGAGGATG AGGAGAAATT TCTCTATGGG GATGAAGAAG AGGATATAAA ATCAGAATCT 
CCACTTAAGT CCCTGGAGGA CCCCGAGAGT GCAGGTACGA GACAGAAGGC AAACTCTCTG 
CCCTCAACTC CAGCTGTAAA GCTAGAATCA CTAGAAGAAA GCAATCCAGA ATATGCCAAG 
ATTCACAACT TGCTTAAGAC CATAGGGCTG GATATTGGAG TAGCAGAAAT TGGTAAATTG 
GCTGCACGAA CCCAGGAGAG ACTTCATGGC AAGAAGCCAT CATCACGCCC CTCAGCTGAC 
CGACGTTTGT CAGCTGACCG ACACTTGTCT GGTGACCGAC ACTTTTCAGC TGACCGCTGT 
TCCTCAGTTG AGCACAGTTT CACAGCTGAT TGGCGCTCAT CAGACCCTCA CAGACCGGAG 
AGTAGGGAGA CACATCATAG CAATACTCAG TCCCCAGAAG TATCTCATCC ACACCCAGCT 
TCCCCGGTGG ATCCTTACTT GCGCACCAAA AATAGCCCTC CATTCCTAAA GTCTGACCAT 
CCAGTGTGCC ATGTTTCAGG ACCAGAGGTA GTTGGCAGTG GGTTTCAATC ATCTGTTGCT 
GTTCGGTGCA TGTTGCCATC AGCCCCATCT ACCCCAATTA GACTTCCTCA CTCTGCTGCG 
TTGTCTCAGT TTCATATACC AGGGGCCTCT CAGTTTGCTG CAGCTCGGAT ACCTCCAAAT 
TATCAGGGAT CTGTCATTCC CTCTGCCTCC TTTGATGCAT ATAGGCACTA CATGGCATAT 
GCAGCCTCAA GGTGGCCTAT GTATCCTGCC TCTCAACCAC CAAGCCACCC TTTATCTGAC 
CCTCATAGGC TATTACCAGT AACCAAACAA GCTGCTCGTA GTCGTCCCAA TCTCCGGGTG 
ATCCCTACTG TGACTCCTGC AAAGCCCAAA CAAGAAATAC CAGTACTAGG CTCAATTTCT 
GTAAAGCGAA TTCCTGTTCG GGTGTCCATT CCATCACTCA TAAAATATAA TCCAAAGAAG 
ATCTCTGATG AGAAGAACCG TGCCTCCCAG AAGCAGAAGG TTATTGAAGA GAGAGAAAAA 
CTGAAAACTG AACAGGAAGC CCGGCAAAAG AAAATGTTTT ATCTCACGAC TGAGTTGGAG 
CGGCTCCATA AACAGCAAGG GGAGATGTTG CGTAAGAAGC GAAGGGAGAA GGATGGTCAC 
AAAGATCCAC TTCTGATGGA GGTGAGTCGA CTTCAGGATA GTATTATGAA GGACATTGCC 
GAGCTACATA AAGAGACAGA AGAAGCAGAA AAGAAGCAGT CTGAACTGGA CAAAGTGGCT 
CAGATCTTGG GAATTGATAT CTTTGATAAA TCCCTGAAAT CTTCAAATGA CAGTAAAGAG 
TCTACAGAGA AGCCTGAAAA AGAAAAATCT AAGAGCCCAG AAAAAGAGTT GTCACCCTCA 
AACTCTTCCT CTAGCAACAA GGAATCAAAA ATGAATGAAA AGTCCTGTAT TAAGAGCCCT 
AGTTCTACTG AAAGCCTCCA ACCAACTGTT AAGCAGTCTG ACCAGCCTGT TGCTGCTTAT 
GAGTATTATG ATGCTGGCAG TCACTGGTGC AAAGACTGCA ATACCACCTG TGGGACCATG 
TTTGACTTCT TCACCCATAT GCACAATAAG AAGCACACAC AGACACTGGA TCCCTACAAC 
AGACCTTGGG CTTCAAAGAC CCAGAGTGAG GCTAAGCAAG ACACTGTAAA GCGAACTGAC 
AAGATAACTG TTCCTGCAAA AGGCTCTGAG TTTCTGATTC CTGTCACTGG GTTTTACTGC 
CAACTCTGTG AGGAATTTTT GGGGGATCCA ATTTCTGGAG AGCAGCATGT CAAGGGTCAC 
CAGCACAACG AAAACTACAA GAAATATGTG GAAGAAAACC CATTGTATGA AGAACGAAGA 
AATCTGGACC GCCAAGCTGG CTTGGCTGTC GTTCTAGAGA CAGAGCGGCG ACGGCAAAAT 
GAGCTAAAAC GCAAACTTAA TGAGAAACCA AAGGAGGAGA AGATAGAAAA AAAGGCAAGA 
ATTGTGAGAG AAGTAAAGGA AGATGACAAA GCCCCTGGAG AACTAGAGGA GCAGCTCTCG 
GAGGATGGGA GTGCCCCTGA GAAAGGTGAG GTGAAGGGGA ACGCTAGCCT CAGACCCCAG 
GTGAAAGAAG AGGTAAAGAA GGAGCCATCA GTAGCATCAA TAGCAGCTTC TTTTGGAAAG 
TTCAGTTGGA AGAAGCCAGA AAAAGAAGAA GAAAAAGGTT CAGTGGTGAC CCCAGGGGCC 
CCAAAGGAAG ACACGGTGGA AACCAGTAAG GACAGAGATG ACGGCAAAGC TGAAGTTGGA 
AAGGCAAAGC CCATCAAAAT CAAGCTTTCT GGAAAAACTG TCATTGCACA CACCAGTCCC 
TGGACACCTG TCGTTACGAC TTCAACACAG ACTAAGATCC GACCCAACTT GCCTATCCCA 
TCCACAGTAC TCCGCAAGTC AGGTTCAGCC ACAGTGAGCA AGCCTGCTCC TCTTAATACC 
TTTCTGTCTA TCAAGTCCTC TGGAACATCC ACCAAGCCTC TGCCAGTGGT TAAAGAGTCA 
TCATCAGATC TTCTCTTGCC TCCAGATATC ATCTCCAAAG CTTTTGGAGG AGAAGAGGTG 
GTTCTAAAAG GGTCTCCAGA GGAAAAAGTG GAACTGGCTG AAAAGAATGA GCCATCCCAA 
GTACCTGAGC AAATGCTGGC TCTACTTCCA CCGCCACCAC CTCCACCTCC ACCCCCACCT 
CCACCTCCAC CCCCTCCACC ACCCCAGGCT GTACCTCAGC TATCTGCCCC ATCCCCTGCT 
CAAGCAAATG TTGTCTTAAC TCCAGTTAAA TCTAATTCGG TGATATCTCA GACTTTCAGT 
CTTGGGTTCC AAGGTCCTAA CATTTTGAAC CCAGGGTTGC CTGTGGCCTT CATGGCCTCA 
GAACAACCAA CTGTAATTCC TTCTGATGAG ACAGCTCCTG GGGTGAGTGA GAGTGACTGG 
GACCAGACCC TAATCTCTAT GTTAGTACGG CCTCCACCAC CCCTTTCCAG TGTATTCAGT 
GAACAGGCCA AAAAATTAGA AAAGCGAAAT TCGTGCTTAG CCACAGCCAA TGCTAAGGAC 
CTGTATGATA TATTCTACAG TAGTGGTGGA AAGGGGGCCC ATGAGACTAA GCTGAGTAGT 
AGTACATTAG CCAATGGGGA AAGCAGTAGC CTTCCTAGAA CAGAAAGTTC AGATTTCTCC 
TCCACTTGTA CTTTGAACAG TAGTATGTCC TCAGAGGACC TGCCTCAATG TAGTGCTTTG 
GTCACTGCTA CTGAAATCAG TAATCTTGAA AATCCTATTT CAAAAGGTAT GGAGTCCACA 
GGGAAATGGT CTGTTGTAGA TCAGATAGAT CCCAAAAGCA GAGACAGCAC CTATAGCTTC 
CTACAGCCTC TGACAAGATT ATACCAAAAT AAGCCTTATG AAATAGTTTC CCCAAAGACA 
GACACTTTGG TCATGTGGAC TTCTGGTTCT TCCCAGAATG ACACTCATAA AGATAGACCT 
CCAGAGGGGA AAATCAGATT TGACCTGGGA GAGCCAGGGC CACCTGGTAC AGATTCAACT 
TCTCATCTAT CAGATACACA CTGTCAAACC AACGGACCTC AGAAGTTGAT AGAAATTAAC 
CTCATAGATA ACCAAAACAA GAACCAAGAG GTATACCAGT CAGAGGGTTG CAGAGAAAGT 
GAAATGAAGA GAAAGACTGA ATTAAAAGGA AAGGTAGCAA CTGAAGAAGA AGAAGAAGAA 
GAAGAAGAAG GTGCCAACAG CATAGAAGAT TCTAATTCAA ACCATGGCAA CAGAAATACA 
TGGGAAGGAG AAATAGGACA ACCCAAGTTG TCAACAGTTG ACAAAAAGGG AGAACAGTCT 
AGCAAATTGA TGACCGGACA TGAGAATACA AGTAAAGTAG TAATTGAATT GAGTCCGTCT 
CTCCCTTCCA AAAGGACAAA AATAGATTTA TTTCCGTCAT TGCTCCAGAA TCCAAAAAGC 
ATGCCTGAAC TACTTCTGCT GTCTCCAGCT GGATCAGGTT TGTGTTTAAA GAGACAAGAG 
ATTTGGGAGA GGCCAGAAAA ACCAGGATTG GAAGATGTGG AGCTACAAGG TACCCGTCCA 
GAACTAACTG TGACAATAGA AAGCAAAGTT TTGGAAAACT TTGACACTAC ACACTTAGAG 
GTAGAGGGGT TTGCATCTCT GAGGAATCTG GGGGATATGC ATGCCAATTT CCACAATAGT 
CAGACAGAAC AGACACGGAG GTCACCAACT GCCCTGTCAG AGAAAATGTC TGAAGAAATT 
TCTGTGTCAT CTGTCATGTG TAATCCTTCC AGTTCCAGTG ACATTGAACC AGTACCATCT 
TTTTCTGGAT TTCCATTAGA ATCTCCCAAA ACTTTGGTGC TTAACTTTGA AACAGAGGGT 
GCACATAGCT CATCTAATTC CAGAAATGGG AGAATCACCT CTAACTCTTT GGAAACTGGA 
CACCCTGTGG AAAATGTTGG CCACGACTTA GGAGGTGAGA GAACACACCA GGCCCTTGAC 
CTCTTAGCTG GAGGAATGTT GTCTGAGGAC GTAAAAGAAA CTTCGCCACT ACAGAAAGAT 
TTACTCAGAA TGGAATCCAC CACAGTCAGT CCTTCAGGCC TTGGACCATC TCCTTGCCTT 
CCAGACCTTG TTGATTTTGT CACACGGACA CCTGGAGTTC CAAAACAAAA ACCATGTTCT 
CCACTTTCTG AGCCAGATGC CTTTCTCAAG TGTAGTTCAC TGGAGATGGG ATCACCACCT 
CCAGAAATTC TGAGTGTGTC TGTCTCAGAG GTAGCAGTTC CTCAAGTAAG TGAAGACAAT 
GACAGTGCTT TGAATTTGGT AAAAACTCCA CCCTCAGGCT CCCCCTCAAG GGATCAGGTA 
GTTGGAGGCA ATGTGAGCCC TCGGGAAATG CCTGAACAAG AAGCTGCAGT TGATGTCATC 
CCAGACCACA CAAGGTCCAA TGTTTATAAC AGTCAGGACT ACTTAAATGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp318 ( NM_021346.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067321.2 Mus musculus zinc finger protein 318 (Zfp318), transcript variant 2, mRNA.

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CloneID	OMu12213
Clone ID Related Accession (Same CDS sequence)	NM_021346.2
Accession Version	NM_021346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	zinc finger protein 318 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT009572.21 and AC151275.3. On Oct 3, 2007 this sequence version replaced NM_021346.1. Transcript Variant: This variant (2) lacks three exons and includes an alternate 3' terminal exon, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACCGCA GTGGCTCCCG CTCCTCCGTC TCTTCGCACC GGTCGAAAGA CGGCAGCGCG 
AGCGGCCCTC CGCCGGGCCG TCCCGTGGGC GCCTCCTCAG GCCCCACACG CCGCCCCTCG 
TCGCCGCCGC CGCCCTCTTG CTCCTCGCTG CGGCTCCCCG CCCGCCGCCA CCGCTCGCCC 
TCAGGCCACC GCGGCCGCTG GGCCTCGCCG TCCCCGCCTC GGGGTCGTCG CGGCTCCCCG 
TCCCCGCCCC GCGGCCGCCG GGCCTCACCG TCTCCGACCC GCGGCCGCCG GGCCTCGCCA 
TCCCCTCCGC GGGGTCGTCG TGGCTCCCCG TCCCCGCCGC GCGCCCGTCG CGGCTCCCCG 
TCGCCACCGC GGAGCCGGCG ACACTACCCA CCTGGCCTGG GTGGCTTCCG AGGCAGCATT 
CGAGGCGAGT CCCGCGCCGA CTTCGCCCGG GACGGCCGCG GAGACCATCC AGGCGGCGGC 
GGCGGCAGTC GGAGACGTTC TCCTGGTCTG TGTTCTGATT CTTCTTTGGA GGAGAGTTTA 
AGGATCACAG TTGGCAATGA CCACTTCTGT GTTAGCACAC CAGAGCGGCG GCGGCTCAGT 
GACCGGCTGG GATCTCCAGT GGATGGTCTG CAGGACATGG ACAGGGATGA CCTGACTGAT 
GATTCTGTCT TCACTCGAAG CTCCCAGTGT TCTCGGGGTC TTGAACGATA CATTTCTCGG 
GAGGAGGGAC CACTTAGTCC CTTCTTGGGA CAACTTGATG AGGACTACCG AACAAGAGAA 
ACTTTTCTGC ATCGACCTGA GTTTAGTCCC CAGAGCAGTT GTCATGATGA ATTGTTGAGG 
GGAACAGAGC GGAATAGAGA CAAACTTAAA AGCTCCTCCT ATTCCATACG ATCTGAGGAA 
AGAAGCCGGG AGGCCAAAAG GCCCCGTTAT GATGACACAG AGAAGGTACA CAGCTCAGGA 
GGGGATCACT CAAGTTTTAC ATCTGGGACT CGAAACTATC GGCAGCGAAG AAGCAGCCCA 
AGCCCTAGGT TTCTAGATCC CGAGTTTCGA GAACTGGACC TTGCCAGGCG AAAGCGAGAG 
GAAGAAGAGG AACAAAGTAG GAGCTTGAGT CAAGAGCTGG TGGGTGTTGG TGATGATCAG 
ATTGGCTGTT CCATCCCTGG CTTGGCAGGA GTCCTAACCA CATCAGAGCC TGGATATTCT 
TTGCAGCGGC CTGAGGAAGT ACCTATGATG CCCAAGAAGT CCATTCTGAA GAAACGAATT 
GAAGCGGACA TGAAGCCTTC CTTGCAGCTT GAGAGCTTTT CTAGCGGTGC CAGCTCTGGT 
GAAGATCACC CTCTTTATTC TGAACATTCA CCTCTTCCTC TAAGTGGTGC TATTGCTGCT 
TTTACTTCAG AGATTGAAAA CAAGGGGACT ACAGTGGAGG CTGACCTGAA AGAGCCTCAG 
AGTAACCTAT ACCAGTGGGG CCCCCTCCGT GAGATACCTA AAGACAACAG TGAAAAATTT 
GACAGTTTTC TAGGCTTTAA GGAAAAATTG GATTTGAAGG CTGAGGGACT TGAGCAACAG 
ACAGATTTCT TACTGCCCCA TGAGAGAGCT AGCCAGGACG GCAGTGGCTT TTCTCGAATT 
CTGAGCATGT TGGCTGATCC TACCATTACA CAGGAAAAAA GAAGACGTAG CTTCCCTGAT 
ATTGAGGATG AGGAGAAATT TCTCTATGGG GATGAAGAAG AGGATATAAA ATCAGAATCT 
CCACTTAAGT CCCTGGAGGA CCCCGAGAGT GCAGGTACGA GACAGAAGGC AAACTCTCTG 
CCCTCAACTC CAGCTGTAAA GCTAGAATCA CTAGAAGAAA GCAATCCAGA ATATGCCAAG 
ATTCACAACT TGCTTAAGAC CATAGGGCTG GATATTGGAG TAGCAGAAAT TGGTAAATTG 
GCTGCACGAA CCCAGGAGAG ACTTCATGGC AAGAAGCCAT CATCACGCCC CTCAGCTGAC 
CGACGTTTGT CAGCTGACCG ACACTTGTCT GGTGACCGAC ACTTTTCAGC TGACCGCTGT 
TCCTCAGTTG AGCACAGTTT CACAGCTGAT TGGCGCTCAT CAGACCCTCA CAGACCGGAG 
AGTAGGGAGA CACATCATAG CAATACTCAG TCCCCAGAAG TATCTCATCC ACACCCAGCT 
TCCCCGGTGG ATCCTTACTT GCGCACCAAA AATAGCCCTC CATTCCTAAA GTCTGACCAT 
CCAGTGTGCC ATGTTTCAGG ACCAGAGGTA GTTGGCAGTG GGTTTCAATC ATCTGTTGCT 
GTTCGGTGCA TGTTGCCATC AGCCCCATCT ACCCCAATTA GACTTCCTCA CTCTGCTGCG 
TTGTCTCAGT TTCATATACC AGGGGCCTCT CAGTTTGCTG CAGCTCGGAT ACCTCCAAAT 
TATCAGGGAT CTGTCATTCC CTCTGCCTCC TTTGATGCAT ATAGGCACTA CATGGCATAT 
GCAGCCTCAA GGTGGCCTAT GTATCCTGCC TCTCAACCAC CAAGCCACCC TTTATCTGAC 
CCTCATAGGC TATTACCAGT AACCAAACAA GCTGCTCGTA GTCGTCCCAA TCTCCGGGTG 
ATCCCTACTG TGACTCCTGC AAAGCCCAAA CAAGAAATAC CAGTACTAGG CTCAATTTCT 
GTAAAGCGAA TTCCTGTTCG GGTGTCCATT CCATCACTCA TAAAATATAA TCCAAAGAAG 
ATCTCTGATG AGAAGAACCG TGCCTCCCAG AAGCAGAAGG TTATTGAAGA GAGAGAAAAA 
CTGAAAACTG AACAGGAAGC CCGGCAAAAG AAAATGTTTT ATCTCACGAC TGAGTTGGAG 
CGGCTCCATA AACAGCAAGG GGAGATGTTG CGTAAGAAGC GAAGGGAGAA GGATGGTCAC 
AAAGATCCAC TTCTGATGGA GGTGAGTCGA CTTCAGGATA GTATTATGAA GGACATTGCC 
GAGCTACATA AAGAGACAGA AGAAGCAGAA AAGAAGCAGT CTGAACTGGA CAAAGTGGCT 
CAGATCTTGG GAATTGATAT CTTTGATAAA TCCCTGAAAT CTTCAAATGA CAGTAAAGAG 
TCTACAGAGA AGCCTGAAAA AGAAAAATCT AAGAGCCCAG AAAAAGAGTT GTCACCCTCA 
AACTCTTCCT CTAGCAACAA GGAATCAAAA ATGAATGAAA AGTCCTGTAT TAAGAGCCCT 
AGTTCTACTG AAAGCCTCCA ACCAACTGTT AAGCAGTCTG ACCAGCCTGT TGCTGCTTAT 
GAGTATTATG ATGCTGGCAG TCACTGGTGC AAAGACTGCA ATACCACCTG TGGGACCATG 
TTTGACTTCT TCACCCATAT GCACAATAAG AAGCACACAC AGGGGCAGTT CCAGAAGTCT 
TCACATTTTC AAACGGAAGG ACTGAAGCAG ATGTTTCTGC TCCAGGAATG CAGGGATAGA 
AACCACAGAG ATTATGGAAA TAATGTGCGT CCTTGTGGCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067321.2
CDS	74..3538
Translation	MYRSGSRSSVSSHRSKDGSASGPPPGRPVGASSGPTRRPSSPPPPSCSSLRLPARRHRSPSGHRGRWASPSPPRGRRGSPSPPRGRRASPSPTRGRRASPSPPRGRRGSPSPPRARRGSPSPPRSRRHYPPGLGGFRGSIRGESRADFARDGRGDHPGGGGGSRRRSPGLCSDSSLEESLRITVGNDHFCVSTPERRRLSDRLGSPVDGLQDMDRDDLTDDSVFTRSSQCSRGLERYISREEGPLSPFLGQLDEDYRTRETFLHRPEFSPQSSCHDELLRGTERNRDKLKSSSYSIRSEERSREAKRPRYDDTEKVHSSGGDHSSFTSGTRNYRQRRSSPSPRFLDPEFRELDLARRKREEEEEQSRSLSQELVGVGDDQIGCSIPGLAGVLTTSEPGYSLQRPEEVPMMPKKSILKKRIEADMKPSLQLESFSSGASSGEDHPLYSEHSPLPLSGAIAAFTSEIENKGTTVEADLKEPQSNLYQWGPLREIPKDNSEKFDSFLGFKEKLDLKAEGLEQQTDFLLPHERASQDGSGFSRILSMLADPTITQEKRRRSFPDIEDEEKFLYGDEEEDIKSESPLKSLEDPESAGTRQKANSLPSTPAVKLESLEESNPEYAKIHNLLKTIGLDIGVAEIGKLAARTQERLHGKKPSSRPSADRRLSADRHLSGDRHFSADRCSSVEHSFTADWRSSDPHRPESRETHHSNTQSPEVSHPHPASPVDPYLRTKNSPPFLKSDHPVCHVSGPEVVGSGFQSSVAVRCMLPSAPSTPIRLPHSAALSQFHIPGASQFAAARIPPNYQGSVIPSASFDAYRHYMAYAASRWPMYPASQPPSHPLSDPHRLLPVTKQAARSRPNLRVIPTVTPAKPKQEIPVLGSISVKRIPVRVSIPSLIKYNPKKISDEKNRASQKQKVIEEREKLKTEQEARQKKMFYLTTELERLHKQQGEMLRKKRREKDGHKDPLLMEVSRLQDSIMKDIAELHKETEEAEKKQSELDKVAQILGIDIFDKSLKSSNDSKESTEKPEKEKSKSPEKELSPSNSSSSNKESKMNEKSCIKSPSSTESLQPTVKQSDQPVAAYEYYDAGSHWCKDCNTTCGTMFDFFTHMHNKKHTQGQFQKSSHFQTEGLKQMFLLQECRDRNHRDYGNNVRPCGQ

Target ORF information:

RefSeq Version	NM_021346.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 318 (Zfp318), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021346.2

ORF Insert Sequence:

ATGTACCGCA GTGGCTCCCG CTCCTCCGTC TCTTCGCACC GGTCGAAAGA CGGCAGCGCG 
AGCGGCCCTC CGCCGGGCCG TCCCGTGGGC GCCTCCTCAG GCCCCACACG CCGCCCCTCG 
TCGCCGCCGC CGCCCTCTTG CTCCTCGCTG CGGCTCCCCG CCCGCCGCCA CCGCTCGCCC 
TCAGGCCACC GCGGCCGCTG GGCCTCGCCG TCCCCGCCTC GGGGTCGTCG CGGCTCCCCG 
TCCCCGCCCC GCGGCCGCCG GGCCTCACCG TCTCCGACCC GCGGCCGCCG GGCCTCGCCA 
TCCCCTCCGC GGGGTCGTCG TGGCTCCCCG TCCCCGCCGC GCGCCCGTCG CGGCTCCCCG 
TCGCCACCGC GGAGCCGGCG ACACTACCCA CCTGGCCTGG GTGGCTTCCG AGGCAGCATT 
CGAGGCGAGT CCCGCGCCGA CTTCGCCCGG GACGGCCGCG GAGACCATCC AGGCGGCGGC 
GGCGGCAGTC GGAGACGTTC TCCTGGTCTG TGTTCTGATT CTTCTTTGGA GGAGAGTTTA 
AGGATCACAG TTGGCAATGA CCACTTCTGT GTTAGCACAC CAGAGCGGCG GCGGCTCAGT 
GACCGGCTGG GATCTCCAGT GGATGGTCTG CAGGACATGG ACAGGGATGA CCTGACTGAT 
GATTCTGTCT TCACTCGAAG CTCCCAGTGT TCTCGGGGTC TTGAACGATA CATTTCTCGG 
GAGGAGGGAC CACTTAGTCC CTTCTTGGGA CAACTTGATG AGGACTACCG AACAAGAGAA 
ACTTTTCTGC ATCGACCTGA GTTTAGTCCC CAGAGCAGTT GTCATGATGA ATTGTTGAGG 
GGAACAGAGC GGAATAGAGA CAAACTTAAA AGCTCCTCCT ATTCCATACG ATCTGAGGAA 
AGAAGCCGGG AGGCCAAAAG GCCCCGTTAT GATGACACAG AGAAGGTACA CAGCTCAGGA 
GGGGATCACT CAAGTTTTAC ATCTGGGACT CGAAACTATC GGCAGCGAAG AAGCAGCCCA 
AGCCCTAGGT TTCTAGATCC CGAGTTTCGA GAACTGGACC TTGCCAGGCG AAAGCGAGAG 
GAAGAAGAGG AACAAAGTAG GAGCTTGAGT CAAGAGCTGG TGGGTGTTGG TGATGATCAG 
ATTGGCTGTT CCATCCCTGG CTTGGCAGGA GTCCTAACCA CATCAGAGCC TGGATATTCT 
TTGCAGCGGC CTGAGGAAGT ACCTATGATG CCCAAGAAGT CCATTCTGAA GAAACGAATT 
GAAGCGGACA TGAAGCCTTC CTTGCAGCTT GAGAGCTTTT CTAGCGGTGC CAGCTCTGGT 
GAAGATCACC CTCTTTATTC TGAACATTCA CCTCTTCCTC TAAGTGGTGC TATTGCTGCT 
TTTACTTCAG AGATTGAAAA CAAGGGGACT ACAGTGGAGG CTGACCTGAA AGAGCCTCAG 
AGTAACCTAT ACCAGTGGGG CCCCCTCCGT GAGATACCTA AAGACAACAG TGAAAAATTT 
GACAGTTTTC TAGGCTTTAA GGAAAAATTG GATTTGAAGG CTGAGGGACT TGAGCAACAG 
ACAGATTTCT TACTGCCCCA TGAGAGAGCT AGCCAGGACG GCAGTGGCTT TTCTCGAATT 
CTGAGCATGT TGGCTGATCC TACCATTACA CAGGAAAAAA GAAGACGTAG CTTCCCTGAT 
ATTGAGGATG AGGAGAAATT TCTCTATGGG GATGAAGAAG AGGATATAAA ATCAGAATCT 
CCACTTAAGT CCCTGGAGGA CCCCGAGAGT GCAGGTACGA GACAGAAGGC AAACTCTCTG 
CCCTCAACTC CAGCTGTAAA GCTAGAATCA CTAGAAGAAA GCAATCCAGA ATATGCCAAG 
ATTCACAACT TGCTTAAGAC CATAGGGCTG GATATTGGAG TAGCAGAAAT TGGTAAATTG 
GCTGCACGAA CCCAGGAGAG ACTTCATGGC AAGAAGCCAT CATCACGCCC CTCAGCTGAC 
CGACGTTTGT CAGCTGACCG ACACTTGTCT GGTGACCGAC ACTTTTCAGC TGACCGCTGT 
TCCTCAGTTG AGCACAGTTT CACAGCTGAT TGGCGCTCAT CAGACCCTCA CAGACCGGAG 
AGTAGGGAGA CACATCATAG CAATACTCAG TCCCCAGAAG TATCTCATCC ACACCCAGCT 
TCCCCGGTGG ATCCTTACTT GCGCACCAAA AATAGCCCTC CATTCCTAAA GTCTGACCAT 
CCAGTGTGCC ATGTTTCAGG ACCAGAGGTA GTTGGCAGTG GGTTTCAATC ATCTGTTGCT 
GTTCGGTGCA TGTTGCCATC AGCCCCATCT ACCCCAATTA GACTTCCTCA CTCTGCTGCG 
TTGTCTCAGT TTCATATACC AGGGGCCTCT CAGTTTGCTG CAGCTCGGAT ACCTCCAAAT 
TATCAGGGAT CTGTCATTCC CTCTGCCTCC TTTGATGCAT ATAGGCACTA CATGGCATAT 
GCAGCCTCAA GGTGGCCTAT GTATCCTGCC TCTCAACCAC CAAGCCACCC TTTATCTGAC 
CCTCATAGGC TATTACCAGT AACCAAACAA GCTGCTCGTA GTCGTCCCAA TCTCCGGGTG 
ATCCCTACTG TGACTCCTGC AAAGCCCAAA CAAGAAATAC CAGTACTAGG CTCAATTTCT 
GTAAAGCGAA TTCCTGTTCG GGTGTCCATT CCATCACTCA TAAAATATAA TCCAAAGAAG 
ATCTCTGATG AGAAGAACCG TGCCTCCCAG AAGCAGAAGG TTATTGAAGA GAGAGAAAAA 
CTGAAAACTG AACAGGAAGC CCGGCAAAAG AAAATGTTTT ATCTCACGAC TGAGTTGGAG 
CGGCTCCATA AACAGCAAGG GGAGATGTTG CGTAAGAAGC GAAGGGAGAA GGATGGTCAC 
AAAGATCCAC TTCTGATGGA GGTGAGTCGA CTTCAGGATA GTATTATGAA GGACATTGCC 
GAGCTACATA AAGAGACAGA AGAAGCAGAA AAGAAGCAGT CTGAACTGGA CAAAGTGGCT 
CAGATCTTGG GAATTGATAT CTTTGATAAA TCCCTGAAAT CTTCAAATGA CAGTAAAGAG 
TCTACAGAGA AGCCTGAAAA AGAAAAATCT AAGAGCCCAG AAAAAGAGTT GTCACCCTCA 
AACTCTTCCT CTAGCAACAA GGAATCAAAA ATGAATGAAA AGTCCTGTAT TAAGAGCCCT 
AGTTCTACTG AAAGCCTCCA ACCAACTGTT AAGCAGTCTG ACCAGCCTGT TGCTGCTTAT 
GAGTATTATG ATGCTGGCAG TCACTGGTGC AAAGACTGCA ATACCACCTG TGGGACCATG 
TTTGACTTCT TCACCCATAT GCACAATAAG AAGCACACAC AGGGGCAGTT CCAGAAGTCT 
TCACATTTTC AAACGGAAGG ACTGAAGCAG ATGTTTCTGC TCCAGGAATG CAGGGATAGA 
AACCACAGAG ATTATGGAAA TAATGTGCGT CCTTGTGGCC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp318 ( XM_011246554.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244856.1 Mus musculus zinc finger protein 318 (Zfp318), transcript variant X1, mRNA.

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CloneID	OMu69760
Clone ID Related Accession (Same CDS sequence)	XM_011246554.3 , XM_011246554.2
Accession Version	XM_011246554.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	zinc finger protein 318 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246554.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGGG ATGACCTGAC TGATGATTCT GTCTTCACTC GAAGCTCCCA GTGTTCTCGG 
GGTCTTGAAC GATACATTTC TCGGGAGGAG GGACCACTTA GTCCCTTCTT GGGACAACTT 
GATGAGGACT ACCGAACAAG AGAAACTTTT CTGCATCGAC CTGAGTTTAG TCCCCAGAGC 
AGTTGTCATG ATGAATTGTT GAGGGGAACA GAGCGGAATA GAGACAAACT TAAAAGCTCC 
TCCTATTCCA TACGATCTGA GGAAAGAAGC CGGGAGGCCA AAAGGCCCCG TTATGATGAC 
ACAGAGAAGG TACACAGCTC AGGAGGGGAT CACTCAAGTT TTACATCTGG GACTCGAAAC 
TATCGGCAGC GAAGAAGCAG CCCAAGCCCT AGGTTTCTAG ATCCCGAGTT TCGAGAACTG 
GACCTTGCCA GGCGAAAGCG AGAGGAAGAA GAGGAACAAA GTAGGAGCTT GAGTCAAGAG 
CTGGTGGGTG TTGGTGATGA TCAGATTGGC TGTTCCATCC CTGGCTTGGC AGGAGTCCTA 
ACCACATCAG AGCCTGGATA TTCTTTGCAG CGGCCTGAGG AAGTACCTAT GATGCCCAAG 
AAGTCCATTC TGAAGAAACG AATTGAAGCG GACATGAAGC CTTCCTTGCA GCTTGAGAGC 
TTTTCTAGCG GTGCCAGCTC TGGTGAAGAT CACCCTCTTT ATTCTGAACA TTCACCTCTT 
CCTCTAAGTG GTGCTATTGC TGCTTTTACT TCAGAGATTG AAAACAAGGG GACTACAGTG 
GAGGCTGACC TGAAAGAGCC TCAGAGTAAC CTATACCAGT GGGGCCCCCT CCGTGAGATA 
CCTAAAGACA ACAGTGAAAA ATTTGACAGT TTTCTAGGCT TTAAGGAAAA ATTGGATTTG 
AAGGCTGAGG GACTTGAGCA ACAGACAGAT TTCTTACTGC CCCATGAGAG AGCTAGCCAG 
GACGGCAGTG GCTTTTCTCG AATTCTGAGC ATGTTGGCTG ATCCTACCAT TACACAGGAA 
AAAAGAAGAC GTAGCTTCCC TGATATTGAG GATGAGGAGA AATTTCTCTA TGGGGATGAA 
GAAGAGGATA TAAAATCAGA ATCTCCACTT AAGTCCCTGG AGGACCCCGA GAGTGCAGGT 
ACGAGACAGA AGGCAAACTC TCTGCCCTCA ACTCCAGCTG TAAAGCTAGA ATCACTAGAA 
GAAAGCAATC CAGAATATGC CAAGATTCAC AACTTGCTTA AGACCATAGG GCTGGATATT 
GGAGTAGCAG AAATTGGTAA ATTGGCTGCA CGAACCCAGG AGAGACTTCA TGGCAAGAAG 
CCATCATCAC GCCCCTCAGC TGACCGACGT TTGTCAGCTG ACCGACACTT GTCTGGTGAC 
CGACACTTTT CAGCTGACCG CTGTTCCTCA GTTGAGCACA GTTTCACAGC TGATTGGCGC 
TCATCAGACC CTCACAGACC GGAGAGTAGG GAGACACATC ATAGCAATAC TCAGTCCCCA 
GAAGTATCTC ATCCACACCC AGCTTCCCCG GTGGATCCTT ACTTGCGCAC CAAAAATAGC 
CCTCCATTCC TAAAGTCTGA CCATCCAGTG TGCCATGTTT CAGGACCAGA GGTAGTTGGC 
AGTGGGTTTC AATCATCTGT TGCTGTTCGG TGCATGTTGC CATCAGCCCC ATCTACCCCA 
ATTAGACTTC CTCACTCTGC TGCGTTGTCT CAGTTTCATA TACCAGGGGC CTCTCAGTTT 
GCTGCAGCTC GGATACCTCC AAATTATCAG GGATCTGTCA TTCCCTCTGC CTCCTTTGAT 
GCATATAGGC ACTACATGGC ATATGCAGCC TCAAGGTGGC CTATGTATCC TGCCTCTCAA 
CCACCAAGCC ACCCTTTATC TGACCCTCAT AGGCTATTAC CAGTAACCAA ACAAGCTGCT 
CGTAGTCGTC CCAATCTCCG GGTGATCCCT ACTGTGACTC CTGCAAAGCC CAAACAAGAA 
ATACCAGTAC TAGGCTCAAT TTCTGTAAAG CGAATTCCTG TTCGGGTGTC CATTCCATCA 
CTCATAAAAT ATAATCCAAA GAAGATCTCT GATGAGAAGA ACCGTGCCTC CCAGAAGCAG 
AAGGTTATTG AAGAGAGAGA AAAACTGAAA ACTGAACAGG AAGCCCGGCA AAAGAAAATG 
TTTTATCTCA CGACTGAGTT GGAGCGGCTC CATAAACAGC AAGGGGAGAT GTTGCGTAAG 
AAGCGAAGGG AGAAGGATGG TCACAAAGAT CCACTTCTGA TGGAGGTGAG TCGACTTCAG 
GATAGTATTA TGAAGGACAT TGCCGAGCTA CATAAAGAGA CAGAAGAAGC AGAAAAGAAG 
CAGTCTGAAC TGGACAAAGT GGCTCAGATC TTGGGAATTG ATATCTTTGA TAAATCCCTG 
AAATCTTCAA ATGACAGTAA AGAGTCTACA GAGAAGCCTG AAAAAGAAAA ATCTAAGAGC 
CCAGAAAAAG AGTTGTCACC CTCAAACTCT TCCTCTAGCA ACAAGGAATC AAAAATGAAT 
GAAAAGTCCT GTATTAAGAG CCCTAGTTCT ACTGAAAGCC TCCAACCAAC TGTTAAGCAG 
TCTGACCAGC CTGTTGCTGC TTATGAGTAT TATGATGCTG GCAGTCACTG GTGCAAAGAC 
TGCAATACCA CCTGTGGGAC CATGTTTGAC TTCTTCACCC ATATGCACAA TAAGAAGCAC 
ACACAGACAC TGGATCCCTA CAACAGACCT TGGGCTTCAA AGACCCAGAG TGAGGCTAAG 
CAAGACACTG TAAAGCGAAC TGACAAGATA ACTGTTCCTG CAAAAGGCTC TGAGTTTCTG 
ATTCCTGTCA CTGGGTTTTA CTGCCAACTC TGTGAGGAAT TTTTGGGGGA TCCAATTTCT 
GGAGAGCAGC ATGTCAAGGG TCACCAGCAC AACGAAAACT ACAAGAAATA TGTGGAAGAA 
AACCCATTGT ATGAAGAACG AAGAAATCTG GACCGCCAAG CTGGCTTGGC TGTCGTTCTA 
GAGACAGAGC GGCGACGGCA AAATGAGCTA AAACGCAAAC TTAATGAGAA ACCAAAGGAG 
GAGAAGATAG AAAAAAAGGC AAGAATTGTG AGAGAAGTAA AGGAAGATGA CAAAGCCCCT 
GGAGAACTAG AGGAGCAGCT CTCGGAGGAT GGGAGTGCCC CTGAGAAAGG TGAGGTGAAG 
GGGAACGCTA GCCTCAGACC CCAGGTGAAA GAAGAGGTAA AGAAGGAGCC ATCAGTAGCA 
TCAATAGCAG CTTCTTTTGG AAAGTTCAGT TGGAAGAAGC CAGAAAAAGA AGAAGAAAAA 
GGTTCAGTGG TGACCCCAGG GGCCCCAAAG GAAGACACGG TGGAAACCAG TAAGGACAGA 
GATGACGGCA AAGCTGAAGT TGGAAAGGCA AAGCCCATCA AAATCAAGCT TTCTGGAAAA 
ACTGTCATTG CACACACCAG TCCCTGGACA CCTGTCGTTA CGACTTCAAC ACAGACTAAG 
ATCCGACCCA ACTTGCCTAT CCCATCCACA GTACTCCGCA AGTCAGGTTC AGCCACAGTG 
AGCAAGCCTG CTCCTCTTAA TACCTTTCTG TCTATCAAGT CCTCTGGAAC ATCCACCAAG 
CCTCTGCCAG TGGTTAAAGA GTCATCATCA GATCTTCTCT TGCCTCCAGA TATCATCTCC 
AAAGCTTTTG GAGGAGAAGA GGTGGTTCTA AAAGGGTCTC CAGAGGAAAA AGTGGAACTG 
GCTGAAAAGA ATGAGCCATC CCAAGTACCT GAGCAAATGC TGGCTCTACT TCCACCGCCA 
CCACCTCCAC CTCCACCCCC ACCTCCACCT CCACCCCCTC CACCACCCCA GGCTGTACCT 
CAGCTATCTG CCCCATCCCC TGCTCAAGCA AATGTTGTCT TAACTCCAGT TAAATCTAAT 
TCGGTGATAT CTCAGACTTT CAGTCTTGGG TTCCAAGGTC CTAACATTTT GAACCCAGGG 
TTGCCTGTGG CCTTCATGGC CTCAGAACAA CCAACTGTAA TTCCTTCTGA TGAGACAGCT 
CCTGGGGTGA GTGAGAGTGA CTGGGACCAG ACCCTAATCT CTATGTTAGT ACGGCCTCCA 
CCACCCCTTT CCAGTGTATT CAGTGAACAG GCCAAAAAAT TAGAAAAGCG AAATTCGTGC 
TTAGCCACAG CCAATGCTAA GGACCTGTAT GATATATTCT ACAGTAGTGG TGGAAAGGGG 
GCCCATGAGA CTAAGCTGAG TAGTAGTACA TTAGCCAATG GGGAAAGCAG TAGCCTTCCT 
AGAACAGAAA GTTCAGATTT CTCCTCCACT TGTACTTTGA ACAGTAGTAT GTCCTCAGAG 
GACCTGCCTC AATGTAGTGC TTTGGTCACT GCTACTGAAA TCAGTAATCT TGAAAATCCT 
ATTTCAAAAG GTATGGAGTC CACAGGGAAA TGGTCTGTTG TAGATCAGAT AGATCCCAAA 
AGCAGAGACA GCACCTATAG CTTCCTACAG CCTCTGACAA GATTATACCA AAATAAGCCT 
TATGAAATAG TTTCCCCAAA GACAGACACT TTGGTCATGT GGACTTCTGG TTCTTCCCAG 
AATGACACTC ATAAAGATAG ACCTCCAGAG GGGAAAATCA GATTTGACCT GGGAGAGCCA 
GGGCCACCTG GTACAGATTC AACTTCTCAT CTATCAGATA CACACTGTCA AACCAACGGA 
CCTCAGAAGT TGATAGAAAT TAACCTCATA GATAACCAAA ACAAGAACCA AGAGGTATAC 
CAGTCAGAGG GTTGCAGAGA AAGTGAAATG AAGAGAAAGA CTGAATTAAA AGGAAAGGTA 
GCAACTGAAG AAGAAGAAGA AGAAGAAGAA GAAGGTGCCA ACAGCATAGA AGATTCTAAT 
TCAAACCATG GCAACAGAAA TACATGGGAA GGAGAAATAG GACAACCCAA GTTGTCAACA 
GTTGACAAAA AGGGAGAACA GTCTAGCAAA TTGATGACCG GACATGAGAA TACAAGTAAA 
GTAGTAATTG AATTGAGTCC GTCTCTCCCT TCCAAAAGGA CAAAAATAGA TTTATTTCCG 
TCATTGCTCC AGAATCCAAA AAGCATGCCT GAACTACTTC TGCTGTCTCC AGCTGGATCA 
GGTTTGTGTT TAAAGAGACA AGAGATTTGG GAGAGGCCAG AAAAACCAGG ATTGGAAGAT 
GTGGAGCTAC AAGGTACCCG TCCAGAACTA ACTGTGACAA TAGAAAGCAA AGTTTTGGAA 
AACTTTGACA CTACACACTT AGAGGTAGAG GGGTTTGCAT CTCTGAGGAA TCTGGGGGAT 
ATGCATGCCA ATTTCCACAA TAGTCAGACA GAACAGACAC GGAGGTCACC AACTGCCCTG 
TCAGAGAAAA TGTCTGAAGA AATTTCTGTG TCATCTGTCA TGTGTAATCC TTCCAGTTCC 
AGTGACATTG AACCAGTACC ATCTTTTTCT GGATTTCCAT TAGAATCTCC CAAAACTTTG 
GTGCTTAACT TTGAAACAGA GGGTGCACAT AGCTCATCTA ATTCCAGAAA TGGGAGAATC 
ACCTCTAACT CTTTGGAAAC TGGACACCCT GTGGAAAATG TTGGCCACGA CTTAGGAGGT 
GAGAGAACAC ACCAGGCCCT TGACCTCTTA GCTGGAGGAA TGTTGTCTGA GGACGTAAAA 
GAAACTTCGC CACTACAGAA AGATTTACTC AGAATGGAAT CCACCACAGT CAGTCCTTCA 
GGCCTTGGAC CATCTCCTTG CCTTCCAGAC CTTGTTGATT TTGTCACACG GACACCTGGA 
GTTCCAAAAC AAAAACCATG TTCTCCACTT TCTGAGCCAG ATGCCTTTCT CAAGTGTAGT 
TCACTGGAGA TGGGATCACC ACCTCCAGAA ATTCTGAGTG TGTCTGTCTC AGAGGTAGCA 
GTTCCTCAAG TAAGTGAAGA CAATGACAGT GCTTTGAATT TGGTAAAAAC TCCACCCTCA 
GGCTCCCCCT CAAGGGATCA GGTAGTTGGA GGCAATGTGA GCCCTCGGGA AATGCCTGAA 
CAAGAAGCTG CAGTTGATGT CATCCCAGAC CACACAAGGT CCAATGTTTA TAACAGTCAG 
GACTACTTAA ATGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244856.1
CDS	430..6507
Translation	MDRDDLTDDSVFTRSSQCSRGLERYISREEGPLSPFLGQLDEDYRTRETFLHRPEFSPQSSCHDELLRGTERNRDKLKSSSYSIRSEERSREAKRPRYDDTEKVHSSGGDHSSFTSGTRNYRQRRSSPSPRFLDPEFRELDLARRKREEEEEQSRSLSQELVGVGDDQIGCSIPGLAGVLTTSEPGYSLQRPEEVPMMPKKSILKKRIEADMKPSLQLESFSSGASSGEDHPLYSEHSPLPLSGAIAAFTSEIENKGTTVEADLKEPQSNLYQWGPLREIPKDNSEKFDSFLGFKEKLDLKAEGLEQQTDFLLPHERASQDGSGFSRILSMLADPTITQEKRRRSFPDIEDEEKFLYGDEEEDIKSESPLKSLEDPESAGTRQKANSLPSTPAVKLESLEESNPEYAKIHNLLKTIGLDIGVAEIGKLAARTQERLHGKKPSSRPSADRRLSADRHLSGDRHFSADRCSSVEHSFTADWRSSDPHRPESRETHHSNTQSPEVSHPHPASPVDPYLRTKNSPPFLKSDHPVCHVSGPEVVGSGFQSSVAVRCMLPSAPSTPIRLPHSAALSQFHIPGASQFAAARIPPNYQGSVIPSASFDAYRHYMAYAASRWPMYPASQPPSHPLSDPHRLLPVTKQAARSRPNLRVIPTVTPAKPKQEIPVLGSISVKRIPVRVSIPSLIKYNPKKISDEKNRASQKQKVIEEREKLKTEQEARQKKMFYLTTELERLHKQQGEMLRKKRREKDGHKDPLLMEVSRLQDSIMKDIAELHKETEEAEKKQSELDKVAQILGIDIFDKSLKSSNDSKESTEKPEKEKSKSPEKELSPSNSSSSNKESKMNEKSCIKSPSSTESLQPTVKQSDQPVAAYEYYDAGSHWCKDCNTTCGTMFDFFTHMHNKKHTQTLDPYNRPWASKTQSEAKQDTVKRTDKITVPAKGSEFLIPVTGFYCQLCEEFLGDPISGEQHVKGHQHNENYKKYVEENPLYEERRNLDRQAGLAVVLETERRRQNELKRKLNEKPKEEKIEKKARIVREVKEDDKAPGELEEQLSEDGSAPEKGEVKGNASLRPQVKEEVKKEPSVASIAASFGKFSWKKPEKEEEKGSVVTPGAPKEDTVETSKDRDDGKAEVGKAKPIKIKLSGKTVIAHTSPWTPVVTTSTQTKIRPNLPIPSTVLRKSGSATVSKPAPLNTFLSIKSSGTSTKPLPVVKESSSDLLLPPDIISKAFGGEEVVLKGSPEEKVELAEKNEPSQVPEQMLALLPPPPPPPPPPPPPPPPPPPQAVPQLSAPSPAQANVVLTPVKSNSVISQTFSLGFQGPNILNPGLPVAFMASEQPTVIPSDETAPGVSESDWDQTLISMLVRPPPPLSSVFSEQAKKLEKRNSCLATANAKDLYDIFYSSGGKGAHETKLSSSTLANGESSSLPRTESSDFSSTCTLNSSMSSEDLPQCSALVTATEISNLENPISKGMESTGKWSVVDQIDPKSRDSTYSFLQPLTRLYQNKPYEIVSPKTDTLVMWTSGSSQNDTHKDRPPEGKIRFDLGEPGPPGTDSTSHLSDTHCQTNGPQKLIEINLIDNQNKNQEVYQSEGCRESEMKRKTELKGKVATEEEEEEEEEGANSIEDSNSNHGNRNTWEGEIGQPKLSTVDKKGEQSSKLMTGHENTSKVVIELSPSLPSKRTKIDLFPSLLQNPKSMPELLLLSPAGSGLCLKRQEIWERPEKPGLEDVELQGTRPELTVTIESKVLENFDTTHLEVEGFASLRNLGDMHANFHNSQTEQTRRSPTALSEKMSEEISVSSVMCNPSSSSDIEPVPSFSGFPLESPKTLVLNFETEGAHSSSNSRNGRITSNSLETGHPVENVGHDLGGERTHQALDLLAGGMLSEDVKETSPLQKDLLRMESTTVSPSGLGPSPCLPDLVDFVTRTPGVPKQKPCSPLSEPDAFLKCSSLEMGSPPPEILSVSVSEVAVPQVSEDNDSALNLVKTPPSGSPSRDQVVGGNVSPREMPEQEAAVDVIPDHTRSNVYNSQDYLNG

Target ORF information:

RefSeq Version	XM_011246554.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 318 (Zfp318), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246554.2

ORF Insert Sequence:

ATGGACAGGG ATGACCTGAC TGATGATTCT GTCTTCACTC GAAGCTCCCA GTGTTCTCGG 
GGTCTTGAAC GATACATTTC TCGGGAGGAG GGACCACTTA GTCCCTTCTT GGGACAACTT 
GATGAGGACT ACCGAACAAG AGAAACTTTT CTGCATCGAC CTGAGTTTAG TCCCCAGAGC 
AGTTGTCATG ATGAATTGTT GAGGGGAACA GAGCGGAATA GAGACAAACT TAAAAGCTCC 
TCCTATTCCA TACGATCTGA GGAAAGAAGC CGGGAGGCCA AAAGGCCCCG TTATGATGAC 
ACAGAGAAGG TACACAGCTC AGGAGGGGAT CACTCAAGTT TTACATCTGG GACTCGAAAC 
TATCGGCAGC GAAGAAGCAG CCCAAGCCCT AGGTTTCTAG ATCCCGAGTT TCGAGAACTG 
GACCTTGCCA GGCGAAAGCG AGAGGAAGAA GAGGAACAAA GTAGGAGCTT GAGTCAAGAG 
CTGGTGGGTG TTGGTGATGA TCAGATTGGC TGTTCCATCC CTGGCTTGGC AGGAGTCCTA 
ACCACATCAG AGCCTGGATA TTCTTTGCAG CGGCCTGAGG AAGTACCTAT GATGCCCAAG 
AAGTCCATTC TGAAGAAACG AATTGAAGCG GACATGAAGC CTTCCTTGCA GCTTGAGAGC 
TTTTCTAGCG GTGCCAGCTC TGGTGAAGAT CACCCTCTTT ATTCTGAACA TTCACCTCTT 
CCTCTAAGTG GTGCTATTGC TGCTTTTACT TCAGAGATTG AAAACAAGGG GACTACAGTG 
GAGGCTGACC TGAAAGAGCC TCAGAGTAAC CTATACCAGT GGGGCCCCCT CCGTGAGATA 
CCTAAAGACA ACAGTGAAAA ATTTGACAGT TTTCTAGGCT TTAAGGAAAA ATTGGATTTG 
AAGGCTGAGG GACTTGAGCA ACAGACAGAT TTCTTACTGC CCCATGAGAG AGCTAGCCAG 
GACGGCAGTG GCTTTTCTCG AATTCTGAGC ATGTTGGCTG ATCCTACCAT TACACAGGAA 
AAAAGAAGAC GTAGCTTCCC TGATATTGAG GATGAGGAGA AATTTCTCTA TGGGGATGAA 
GAAGAGGATA TAAAATCAGA ATCTCCACTT AAGTCCCTGG AGGACCCCGA GAGTGCAGGT 
ACGAGACAGA AGGCAAACTC TCTGCCCTCA ACTCCAGCTG TAAAGCTAGA ATCACTAGAA 
GAAAGCAATC CAGAATATGC CAAGATTCAC AACTTGCTTA AGACCATAGG GCTGGATATT 
GGAGTAGCAG AAATTGGTAA ATTGGCTGCA CGAACCCAGG AGAGACTTCA TGGCAAGAAG 
CCATCATCAC GCCCCTCAGC TGACCGACGT TTGTCAGCTG ACCGACACTT GTCTGGTGAC 
CGACACTTTT CAGCTGACCG CTGTTCCTCA GTTGAGCACA GTTTCACAGC TGATTGGCGC 
TCATCAGACC CTCACAGACC GGAGAGTAGG GAGACACATC ATAGCAATAC TCAGTCCCCA 
GAAGTATCTC ATCCACACCC AGCTTCCCCG GTGGATCCTT ACTTGCGCAC CAAAAATAGC 
CCTCCATTCC TAAAGTCTGA CCATCCAGTG TGCCATGTTT CAGGACCAGA GGTAGTTGGC 
AGTGGGTTTC AATCATCTGT TGCTGTTCGG TGCATGTTGC CATCAGCCCC ATCTACCCCA 
ATTAGACTTC CTCACTCTGC TGCGTTGTCT CAGTTTCATA TACCAGGGGC CTCTCAGTTT 
GCTGCAGCTC GGATACCTCC AAATTATCAG GGATCTGTCA TTCCCTCTGC CTCCTTTGAT 
GCATATAGGC ACTACATGGC ATATGCAGCC TCAAGGTGGC CTATGTATCC TGCCTCTCAA 
CCACCAAGCC ACCCTTTATC TGACCCTCAT AGGCTATTAC CAGTAACCAA ACAAGCTGCT 
CGTAGTCGTC CCAATCTCCG GGTGATCCCT ACTGTGACTC CTGCAAAGCC CAAACAAGAA 
ATACCAGTAC TAGGCTCAAT TTCTGTAAAG CGAATTCCTG TTCGGGTGTC CATTCCATCA 
CTCATAAAAT ATAATCCAAA GAAGATCTCT GATGAGAAGA ACCGTGCCTC CCAGAAGCAG 
AAGGTTATTG AAGAGAGAGA AAAACTGAAA ACTGAACAGG AAGCCCGGCA AAAGAAAATG 
TTTTATCTCA CGACTGAGTT GGAGCGGCTC CATAAACAGC AAGGGGAGAT GTTGCGTAAG 
AAGCGAAGGG AGAAGGATGG TCACAAAGAT CCACTTCTGA TGGAGGTGAG TCGACTTCAG 
GATAGTATTA TGAAGGACAT TGCCGAGCTA CATAAAGAGA CAGAAGAAGC AGAAAAGAAG 
CAGTCTGAAC TGGACAAAGT GGCTCAGATC TTGGGAATTG ATATCTTTGA TAAATCCCTG 
AAATCTTCAA ATGACAGTAA AGAGTCTACA GAGAAGCCTG AAAAAGAAAA ATCTAAGAGC 
CCAGAAAAAG AGTTGTCACC CTCAAACTCT TCCTCTAGCA ACAAGGAATC AAAAATGAAT 
GAAAAGTCCT GTATTAAGAG CCCTAGTTCT ACTGAAAGCC TCCAACCAAC TGTTAAGCAG 
TCTGACCAGC CTGTTGCTGC TTATGAGTAT TATGATGCTG GCAGTCACTG GTGCAAAGAC 
TGCAATACCA CCTGTGGGAC CATGTTTGAC TTCTTCACCC ATATGCACAA TAAGAAGCAC 
ACACAGACAC TGGATCCCTA CAACAGACCT TGGGCTTCAA AGACCCAGAG TGAGGCTAAG 
CAAGACACTG TAAAGCGAAC TGACAAGATA ACTGTTCCTG CAAAAGGCTC TGAGTTTCTG 
ATTCCTGTCA CTGGGTTTTA CTGCCAACTC TGTGAGGAAT TTTTGGGGGA TCCAATTTCT 
GGAGAGCAGC ATGTCAAGGG TCACCAGCAC AACGAAAACT ACAAGAAATA TGTGGAAGAA 
AACCCATTGT ATGAAGAACG AAGAAATCTG GACCGCCAAG CTGGCTTGGC TGTCGTTCTA 
GAGACAGAGC GGCGACGGCA AAATGAGCTA AAACGCAAAC TTAATGAGAA ACCAAAGGAG 
GAGAAGATAG AAAAAAAGGC AAGAATTGTG AGAGAAGTAA AGGAAGATGA CAAAGCCCCT 
GGAGAACTAG AGGAGCAGCT CTCGGAGGAT GGGAGTGCCC CTGAGAAAGG TGAGGTGAAG 
GGGAACGCTA GCCTCAGACC CCAGGTGAAA GAAGAGGTAA AGAAGGAGCC ATCAGTAGCA 
TCAATAGCAG CTTCTTTTGG AAAGTTCAGT TGGAAGAAGC CAGAAAAAGA AGAAGAAAAA 
GGTTCAGTGG TGACCCCAGG GGCCCCAAAG GAAGACACGG TGGAAACCAG TAAGGACAGA 
GATGACGGCA AAGCTGAAGT TGGAAAGGCA AAGCCCATCA AAATCAAGCT TTCTGGAAAA 
ACTGTCATTG CACACACCAG TCCCTGGACA CCTGTCGTTA CGACTTCAAC ACAGACTAAG 
ATCCGACCCA ACTTGCCTAT CCCATCCACA GTACTCCGCA AGTCAGGTTC AGCCACAGTG 
AGCAAGCCTG CTCCTCTTAA TACCTTTCTG TCTATCAAGT CCTCTGGAAC ATCCACCAAG 
CCTCTGCCAG TGGTTAAAGA GTCATCATCA GATCTTCTCT TGCCTCCAGA TATCATCTCC 
AAAGCTTTTG GAGGAGAAGA GGTGGTTCTA AAAGGGTCTC CAGAGGAAAA AGTGGAACTG 
GCTGAAAAGA ATGAGCCATC CCAAGTACCT GAGCAAATGC TGGCTCTACT TCCACCGCCA 
CCACCTCCAC CTCCACCCCC ACCTCCACCT CCACCCCCTC CACCACCCCA GGCTGTACCT 
CAGCTATCTG CCCCATCCCC TGCTCAAGCA AATGTTGTCT TAACTCCAGT TAAATCTAAT 
TCGGTGATAT CTCAGACTTT CAGTCTTGGG TTCCAAGGTC CTAACATTTT GAACCCAGGG 
TTGCCTGTGG CCTTCATGGC CTCAGAACAA CCAACTGTAA TTCCTTCTGA TGAGACAGCT 
CCTGGGGTGA GTGAGAGTGA CTGGGACCAG ACCCTAATCT CTATGTTAGT ACGGCCTCCA 
CCACCCCTTT CCAGTGTATT CAGTGAACAG GCCAAAAAAT TAGAAAAGCG AAATTCGTGC 
TTAGCCACAG CCAATGCTAA GGACCTGTAT GATATATTCT ACAGTAGTGG TGGAAAGGGG 
GCCCATGAGA CTAAGCTGAG TAGTAGTACA TTAGCCAATG GGGAAAGCAG TAGCCTTCCT 
AGAACAGAAA GTTCAGATTT CTCCTCCACT TGTACTTTGA ACAGTAGTAT GTCCTCAGAG 
GACCTGCCTC AATGTAGTGC TTTGGTCACT GCTACTGAAA TCAGTAATCT TGAAAATCCT 
ATTTCAAAAG GTATGGAGTC CACAGGGAAA TGGTCTGTTG TAGATCAGAT AGATCCCAAA 
AGCAGAGACA GCACCTATAG CTTCCTACAG CCTCTGACAA GATTATACCA AAATAAGCCT 
TATGAAATAG TTTCCCCAAA GACAGACACT TTGGTCATGT GGACTTCTGG TTCTTCCCAG 
AATGACACTC ATAAAGATAG ACCTCCAGAG GGGAAAATCA GATTTGACCT GGGAGAGCCA 
GGGCCACCTG GTACAGATTC AACTTCTCAT CTATCAGATA CACACTGTCA AACCAACGGA 
CCTCAGAAGT TGATAGAAAT TAACCTCATA GATAACCAAA ACAAGAACCA AGAGGTATAC 
CAGTCAGAGG GTTGCAGAGA AAGTGAAATG AAGAGAAAGA CTGAATTAAA AGGAAAGGTA 
GCAACTGAAG AAGAAGAAGA AGAAGAAGAA GAAGGTGCCA ACAGCATAGA AGATTCTAAT 
TCAAACCATG GCAACAGAAA TACATGGGAA GGAGAAATAG GACAACCCAA GTTGTCAACA 
GTTGACAAAA AGGGAGAACA GTCTAGCAAA TTGATGACCG GACATGAGAA TACAAGTAAA 
GTAGTAATTG AATTGAGTCC GTCTCTCCCT TCCAAAAGGA CAAAAATAGA TTTATTTCCG 
TCATTGCTCC AGAATCCAAA AAGCATGCCT GAACTACTTC TGCTGTCTCC AGCTGGATCA 
GGTTTGTGTT TAAAGAGACA AGAGATTTGG GAGAGGCCAG AAAAACCAGG ATTGGAAGAT 
GTGGAGCTAC AAGGTACCCG TCCAGAACTA ACTGTGACAA TAGAAAGCAA AGTTTTGGAA 
AACTTTGACA CTACACACTT AGAGGTAGAG GGGTTTGCAT CTCTGAGGAA TCTGGGGGAT 
ATGCATGCCA ATTTCCACAA TAGTCAGACA GAACAGACAC GGAGGTCACC AACTGCCCTG 
TCAGAGAAAA TGTCTGAAGA AATTTCTGTG TCATCTGTCA TGTGTAATCC TTCCAGTTCC 
AGTGACATTG AACCAGTACC ATCTTTTTCT GGATTTCCAT TAGAATCTCC CAAAACTTTG 
GTGCTTAACT TTGAAACAGA GGGTGCACAT AGCTCATCTA ATTCCAGAAA TGGGAGAATC 
ACCTCTAACT CTTTGGAAAC TGGACACCCT GTGGAAAATG TTGGCCACGA CTTAGGAGGT 
GAGAGAACAC ACCAGGCCCT TGACCTCTTA GCTGGAGGAA TGTTGTCTGA GGACGTAAAA 
GAAACTTCGC CACTACAGAA AGATTTACTC AGAATGGAAT CCACCACAGT CAGTCCTTCA 
GGCCTTGGAC CATCTCCTTG CCTTCCAGAC CTTGTTGATT TTGTCACACG GACACCTGGA 
GTTCCAAAAC AAAAACCATG TTCTCCACTT TCTGAGCCAG ATGCCTTTCT CAAGTGTAGT 
TCACTGGAGA TGGGATCACC ACCTCCAGAA ATTCTGAGTG TGTCTGTCTC AGAGGTAGCA 
GTTCCTCAAG TAAGTGAAGA CAATGACAGT GCTTTGAATT TGGTAAAAAC TCCACCCTCA 
GGCTCCCCCT CAAGGGATCA GGTAGTTGGA GGCAATGTGA GCCCTCGGGA AATGCCTGAA 
CAAGAAGCTG CAGTTGATGT CATCCCAGAC CACACAAGGT CCAATGTTTA TAACAGTCAG 
GACTACTTAA ATGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Abnormal spermatogenesis and male infertility in testicular zinc finger protein Zfp318-knockout mice.
Development, growth & differentiation58(7)600-8(2016 Sep)
Ishizuka M,Ohtsuka E,Inoue A,Odaka M,Ohshima H,Tamura N,Yoshida K,Sako N,Baba T,Kashiwabara S,Okabe M,Noguchi J,Hagiwara H

Zfp318 regulates IgD expression by abrogating transcription termination within the Ighm/Ighd locus.
Journal of immunology (Baltimore, Md. : 1950)193(5)2546-53(2014 Sep)
Pioli PD,Debnath I,Weis JJ,Weis JH

Zinc-finger protein ZFP318 is essential for expression of IgD, the alternatively spliced Igh product made by mature B lymphocytes.
Proceedings of the National Academy of Sciences of the United States of America111(12)4513-8(2014 Mar)
Enders A,Short A,Miosge LA,Bergmann H,Sontani Y,Bertram EM,Whittle B,Balakishnan B,Yoshida K,Sjollema G,Field MA,Andrews TD,Hagiwara H,Goodnow CC

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

Opposite effects of alternative TZF spliced variants on androgen receptor.
Biochemical and biophysical research communications341(2)515-21(2006 Mar)
Tao RH,Kawate H,Ohnaka K,Ishizuka M,Hagiwara H,Takayanagi R

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Zfp318 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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