Vapb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vapb
Entrez Gene ID	56491
Full Name	vesicle-associated membrane protein, associated protein B and C
Synonyms	AI225786,AI840687,AI848259,D2Abb2e,R75548,VAP33b,Vamp33b
General protein information	Preferred Names vesicle-associated membrane protein, associated protein B and C Names vesicle-associated membrane protein-associated protein B VAMP-B VAMP-associated protein 33b VAMP-associated protein B VAP-B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 H4\|2 97.37 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_019806.5	NP_062780.2	vesicle-associated membrane protein-associated protein B

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	MGC76271	NP_988905.1
Homo sapiens (human)	VAPB	NP_004729.1
Mus musculus (house mouse)	Vapb	NP_062780.2
Rattus norvegicus (Norway rat)	Vapb	NP_068619.1
Gallus gallus (chicken)	VAPB	NP_001006296.1
Drosophila melanogaster (fruit fly)	Vap-33-1	NP_996347.1
Canis lupus familiaris (dog)	VAPB	XP_534466.2
Danio rerio (zebrafish)	vapb	NP_997812.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000626	XP_310452.5
Pan troglodytes (chimpanzee)	VAPB	XP_001135464.1
Bos taurus (cattle)	VAPB	NP_001075892.1
Caenorhabditis elegans (roundworm)	vpr-1	NP_491704.1

Gene Ontology
Bibliography

Related articles in PubMed

Vapb/Amyotrophic lateral sclerosis 8 knock-in mice display slowly progressive motor behavior defects accompanying ER stress and autophagic response.
Larroquette F, Seto L, Gaub PL, Kamal B, Wallis D, Larivi?re R, Vall?e J, Robitaille R, Tsuda H
Human molecular genetics24(22)6515-29(2015 Nov)

ALS-Linked P56S-VAPB Mutation Impairs the Formation of Multinuclear Myotube in C2C12 Cells.
Tokutake Y, Yamada K, Ohata M, Obayashi Y, Tsuchiya M, Yonekura S
International journal of molecular sciences16(8)18628-41(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

To identify pathological defects in animals expressing the P56S mutant VAPB protein at physiological levels in the appropriate tissues, we have generated Vapb knock-in mice
Title: Vapb/Amyotrophic lateral sclerosis 8 knock-in mice display slowly progressive motor behavior defects accompanying ER stress and autophagic response.

The disruption of the IRE1-XBP1 pathway is a cause for the reduced myotube formation in P56S-VAPB-mutation expressing cells.
Title: ALS-Linked P56S-VAPB Mutation Impairs the Formation of Multinuclear Myotube in C2C12 Cells.

Mice knocked-out for Vapb showed mild motor deficits after 18 months of age.
Title: Investigating the contribution of VAPB/ALS8 loss of function in amyotrophic lateral sclerosis.

Vapb knock-out mice exhibit abnormal muscular triacylglycerol levels and FoxO target gene transcriptional responses to fasting and refeeding
Title: VAPB/ALS8 MSP ligands regulate striated muscle energy metabolism critical for adult survival in caenorhabditis elegans.

VapB positively regulates RANKL-mediated osteoclastogenesis via PLCgamma2-Ca(2+)-NFAT signaling
Title: VapB as a regulator of osteoclastogenesis via modulation of PLC?2-Ca(2+)-NFAT signaling.

The following Vapb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vapb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14839	NM_019806.5 Latest version!	Mus musculus vesicle-associated membrane protein, associated protein B and C (Vapb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vapb ( NM_019806.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062780.2 Mus musculus vesicle-associated membrane protein, associated protein B and C (Vapb), mRNA.

Price & Availability↑

CloneID	OMu14839
Clone ID Related Accession (Same CDS sequence)	NM_019806.5
Accession Version	NM_019806.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	vesicle-associated membrane protein-associated protein B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC048231.1 and AL844849.13. On Feb 4, 2006 this sequence version replaced NM_019806.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC048231.1, BC057123.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAAGG TGGAACAGGT CCTGAGCCTC GAGCCACAAC ACGAGCTCAA GTTCCGAGGT 
CCCTTCACTG ATGTTGTCAC CACCAACCTA AAGCTTGGCA ACCCAACAGA CCGAAATGTG 
TGTTTTAAAG TGAAGACCAC AGCACCTCGC AGGTACTGCG TGCGGCCCAA CAGTGGGGTC 
ATTGATGCCG GGGCCTCTCT CAATGTGTCT GTGATGTTAC AGCCTTTCGA TTATGATCCC 
AATGAGAAAA GTAAACACAA GTTTATGGTT CAGTCTATGT TTGCTCCGCC TGACACTTCT 
GATATGGAGG CAGTATGGAA GGAGGCAAAA CCGGAAGACC TTATGGATTC AAAACTTAGA 
TGTGTGTTTG AATTGCCAGC AGAAAATGCT AAACCACATG ATGTAGAAAT AAATAAAATC 
ATACCTACGA GTGCATCCAA GACAGAAGCA CCGGCAGCCG CAAAGTCCCT GACATCGCCC 
CTCGATGACA CAGAAGTAAA GAAGGTGATG GAAGAGTGCA GGCGGCTGCA GGGGGAGGTG 
CAGAGGCTTC GGGAGGAGAG CAGGCAGCTC AAGGAAGAAG ACGGACTTCG GGTGAGGAAG 
GCGATGCCGA GCAACAGCCC CGTGGCGGCT CTGGCGGCCA CTGGGAAGGA GGAGGGCCTG 
AGCGCCCGGC TGCTGGCCCT GGTGGTTCTG TTCTTTATCG TTGGTGTCAT TATAGGGAAG 
ATTGCCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062780.2
CDS	123..854
Translation	MAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPRRYCVRPNSGVIDAGASLNVSVMLQPFDYDPNEKSKHKFMVQSMFAPPDTSDMEAVWKEAKPEDLMDSKLRCVFELPAENAKPHDVEINKIIPTSASKTEAPAAAKSLTSPLDDTEVKKVMEECRRLQGEVQRLREESRQLKEEDGLRVRKAMPSNSPVAALAATGKEEGLSARLLALVVLFFIVGVIIGKIAL

Target ORF information:

RefSeq Version	NM_019806.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus vesicle-associated membrane protein, associated protein B and C (Vapb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019806.5

ORF Insert Sequence:

ATGGCGAAGG TGGAACAGGT CCTGAGCCTC GAGCCACAAC ACGAGCTCAA GTTCCGAGGT 
CCCTTCACTG ATGTTGTCAC CACCAACCTA AAGCTTGGCA ACCCAACAGA CCGAAATGTG 
TGTTTTAAAG TGAAGACCAC AGCACCTCGC AGGTACTGCG TGCGGCCCAA CAGTGGGGTC 
ATTGATGCCG GGGCCTCTCT CAATGTGTCT GTGATGTTAC AGCCTTTCGA TTATGATCCC 
AATGAGAAAA GTAAACACAA GTTTATGGTT CAGTCTATGT TTGCTCCGCC TGACACTTCT 
GATATGGAGG CAGTATGGAA GGAGGCAAAA CCGGAAGACC TTATGGATTC AAAACTTAGA 
TGTGTGTTTG AATTGCCAGC AGAAAATGCT AAACCACATG ATGTAGAAAT AAATAAAATC 
ATACCTACGA GTGCATCCAA GACAGAAGCA CCGGCAGCCG CAAAGTCCCT GACATCGCCC 
CTCGATGACA CAGAAGTAAA GAAGGTGATG GAAGAGTGCA GGCGGCTGCA GGGGGAGGTG 
CAGAGGCTTC GGGAGGAGAG CAGGCAGCTC AAGGAAGAAG ACGGACTTCG GGTGAGGAAG 
GCGATGCCGA GCAACAGCCC CGTGGCGGCT CTGGCGGCCA CTGGGAAGGA GGAGGGCCTG 
AGCGCCCGGC TGCTGGCCCT GGTGGTTCTG TTCTTTATCG TTGGTGTCAT TATAGGGAAG 
ATTGCCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Vapb/Amyotrophic lateral sclerosis 8 knock-in mice display slowly progressive motor behavior defects accompanying ER stress and autophagic response.
Human molecular genetics24(22)6515-29(2015 Nov)
Larroquette F,Seto L,Gaub PL,Kamal B,Wallis D,Larivi?re R,Vall?e J,Robitaille R,Tsuda H

ALS-Linked P56S-VAPB Mutation Impairs the Formation of Multinuclear Myotube in C2C12 Cells.
International journal of molecular sciences16(8)18628-41(2015 Aug)
Tokutake Y,Yamada K,Ohata M,Obayashi Y,Tsuchiya M,Yonekura S

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Vapb cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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