Msln cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Msln
Entrez Gene ID	56047
Full Name	mesothelin
Synonyms	MPF
General protein information	Preferred Names mesothelin Names mesothelin megakaryocyte potentiating factor pre-pro-megakaryocyte-potentiating factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 A3.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524649.3	XP_006524712.1	mesothelin isoform X1
XM_006524650.3	XP_006524713.1	mesothelin isoform X2
NM_018857.1	NP_061345.1	mesothelin precursor
NM_001374653.1	NP_001361582.1	mesothelin isoform 2 precursor
NM_001356286.1	NP_001343215.1	mesothelin isoform 1 preproprotein
NM_018857.2	NP_061345.1	mesothelin isoform 1 preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MSLN	XP_003952796.1
Mus musculus (house mouse)	Msln	NP_061345.1
Xenopus tropicalis (tropical clawed frog)	LOC101733400	XP_004918363.1
Homo sapiens (human)	MSLN	NP_001170826.1
Canis lupus familiaris (dog)	MSLN	XP_854112.1
Bos taurus (cattle)	MSLN	NP_001093844.1
Rattus norvegicus (Norway rat)	Msln	NP_113846.1
Gallus gallus (chicken)	MSLN	XP_414835.3
	MSLN	XP_001087333.2
Xenopus tropicalis (tropical clawed frog)	LOC100489413	XP_002935910.2
Xenopus tropicalis (tropical clawed frog)	LOC101733470	XP_004918364.1

Gene Ontology
Bibliography

Related articles in PubMed

Mesothelin/mucin 16 signaling in activated portal fibroblasts regulates cholestatic liver fibrosis.
Koyama Y, Wang P, Liang S, Iwaisako K, Liu X, Xu J, Zhang M, Sun M, Cong M, Karin D, Taura K, Benner C, Heinz S, Bera T, Brenner DA, Kisseleva T
The Journal of clinical investigation127(4)1254-1270(2017 Apr)

Overexpression of sulfatase-1 in murine hepatocarcinoma Hca-F cell line downregulates mesothelin and leads to reduction in lymphatic metastasis, both in vitro and in vivo.
Mahmoud S, Ibrahim M, Hago A, Huang Y, Wei Y, Zhang J, Zhang Q, Xiao Y, Wang J, Adam M, Guo Y, Wang L, Zhou S, Xin B, Xuan W, Tang J
Oncotarget7(46)75052-75063(2016 11)

Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer.
Zervos E, Agle S, Freistaedter AG, Jones GJ, Roper RL
Journal of experimental & clinical cancer research : CR3539(2016 Mar)

T Cells Engineered against a Native Antigen Can Surmount Immunologic and Physical Barriers to Treat Pancreatic Ductal Adenocarcinoma.
Stromnes IM, Schmitt TM, Hulbert A, Brockenbrough JS, Nguyen H, Cuevas C, Dotson AM, Tan X, Hotes JL, Greenberg PD, Hingorani SR
Cancer cell28(5)638-652(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mesothelin/mucin 16 signaling in activated portal fibroblasts regulates cholestatic liver fibrosis.(
Title: Mesothelin/mucin 16 signaling in activated portal fibroblasts regulates cholestatic liver fibrosis.

Results show that mouse mesothelin is similar to human mesothelin. Its stable overexpression in a pancreatic cancer cell line did not increase cell proliferation, suggesting that mesothelin is not necessarily a tumor progression factor.
Title: Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer.

MSLN has an important role in the growth of lung cancer cells in vivo raising the possibility that inactivation of MPF may be a useful treatment for lung and other MSLN expressing cancers.
Title: Megakaryocytic potentiating factor and mature mesothelin stimulate the growth of a lung cancer cell line in the peritoneal cavity of mice.

The mesothelin expression promotes resistance to certain chemotherapy drugs such as TNF-alpha, paclitaxel, and a combination of platinum and cyclophosphamide.
Title: The role of mesothelin in tumor progression and targeted therapy.

Mesothelin overexpression promotes mesothelioma cell invasion and MMP-9 secretion.
Title: Mesothelin overexpression promotes mesothelioma cell invasion and MMP-9 secretion in an orthotopic mouse model and in epithelioid pleural mesothelioma patients.

The following Msln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Msln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11132	NM_001356286.1 Latest version!	Mus musculus mesothelin (Msln), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu11132	NM_018857.2 Latest version!	Mus musculus mesothelin (Msln), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu11132	XM_006524650.3 Latest version!	Mus musculus mesothelin (Msln), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu53845	XM_006524649.3 Latest version!	Mus musculus mesothelin (Msln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu53845	NM_001374653.1 Latest version!	Mus musculus mesothelin (Msln), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu11132	NM_018857.1	Mus musculus mesothelin (Msln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Msln ( NM_018857.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061345.1 Mus musculus mesothelin (Msln), mRNA.

Price & Availability↑

CloneID	OMu11132
Clone ID Related Accession (Same CDS sequence)	XM_006524650.3 , NM_018857.1 , NM_001356286.1 , NM_018857.2
Accession Version	NM_018857.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Mus musculus(House mouse)
Product	mesothelin precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D86370.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86370.1, AK146799.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGT 
CTCCCCACAG GCCTCTTTCT TGGCCTCACA TGTGAGGAGG TATCTGACCT GAGCATGGAA 
CAAGCCAAGG GGCTGGCTAT GGCTGTAAGA CAGAAGAACA TTACACTCCG GGGACATCAG 
CTGCGTTGTC TGGCACGTCG CCTTCCTAGG CACCTCACCG ACGAGGAACT GAATGCTCTT 
CCACTGGACC TGCTGCTCTT CCTCAACCCA GCCATGTTTC CAGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTGGATGTAC TCCCACGGAG GTCTCTGGAG 
CGCCAGAGGC TGCTGATGGA GGCTCTGAAG TGCCAGGGCG TGTATGGATT TCAAGTGAGT 
GAGGCAGATG TGCGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGAA ATTTGTGGCC 
AGATCTTCCG AAGTTCTCCT CCCCTGGCTG GCAGGATGCC AAGGACCCCT GGACCAGAGC 
CAGGAAAAGG CAGTCAGGGA GGTTCTGAGG AGTGGAAGAA CCCAATATGG CCCCCCATCG 
AAGTGGTCAG TCTCCACCCT GGATGCCCTG CAGAGCTTGG TAGCAGTGTT GGATGAGTCC 
ATCGTCCAGA GCATCCCCAA GGATGTCAAA GCTGAATGGC TGCAACACAT CTCCAGAGAC 
CCCTCCAGGC TGGGGTCTAA GCTGACCGTC ATACACCCAA GGTTCCGACG GGATGCAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCC TACAAGGTGG ATGAAGACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTAGAT GGCACCATGC TGGCCAGACA AATGGACCTT 
GTGAACGAGA TTCCCTTCAC CTATGAGCAG CTCAGTATCT TTAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC TGAGTCCCTG ATCCAGCAGC TGGGTCACTT CTTCAGATAT 
GTTAGCCCTG AAGACATCCA CCAGTGGAAT GTGACCTCAC CAGACACAGT GAAAACTCTG 
CTCAAAGTCA GCAAAGGACA AAAGATGAAT GCTCAGGCGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCCAGCT GGACGAGGAT ATGGTAAAAG CCCTGGGCGA CATCCCGTTA 
AGCTATCTAT GTGACTTCAG CCCCCAGGAT CTGCACTCGG TACCCTCCAG TGTCATGTGG 
CTGGTTGGGC CCCAAGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTCT CCTCTACCAG 
AAGGCCTGCT CAGCCTTCCA GAATGTGAGC GGCCTAGAAT ACTTTGAGAA AATCAAGACA 
TTCCTGGGTG GGGCCTCCGT GAAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGCATG 
GACATAGCCA CTTTCAAGAG GCTGCAGGTG GATTCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTGGGGCC AAACATTGTG GACCTGAAGA CCGAGGAGGA TAAAAGCCCT 
GTCCGTGACT GGCTGTTCCG GCAGCATCAG AAAGACCTAG ACAGGCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGGTC CTGGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGCAGAGCCT CACTCCTTGG GCCAGGATTT GTATTAATAT GGATTCCAGC TCTGCTCCCA 
GCTTTAAGGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061345.1
CDS	109..1986
Translation	MALPTARPLLGSCGSPICSRSFLLLLLSLGWIPRLQTQTTKTSQEATLLHAVNGAADFASLPTGLFLGLTCEEVSDLSMEQAKGLAMAVRQKNITLRGHQLRCLARRLPRHLTDEELNALPLDLLLFLNPAMFPGQQACAHFFSLISKANVDVLPRRSLERQRLLMEALKCQGVYGFQVSEADVRALGGLACDLPGKFVARSSEVLLPWLAGCQGPLDQSQEKAVREVLRSGRTQYGPPSKWSVSTLDALQSLVAVLDESIVQSIPKDVKAEWLQHISRDPSRLGSKLTVIHPRFRRDAEQKACPPGKEPYKVDEDLIFYQNWELEACVDGTMLARQMDLVNEIPFTYEQLSIFKHKLDKTYPQGYPESLIQQLGHFFRYVSPEDIHQWNVTSPDTVKTLLKVSKGQKMNAQAIALVACYLRGGGQLDEDMVKALGDIPLSYLCDFSPQDLHSVPSSVMWLVGPQDLDKCSQRHLGLLYQKACSAFQNVSGLEYFEKIKTFLGGASVKDLRALSQHNVSMDIATFKRLQVDSLVGLSVAEVQKLLGPNIVDLKTEEDKSPVRDWLFRQHQKDLDRLGLGLQGGIPNGYLVLDFNVREAFSSRASLLGPGFVLIWIPALLPALRLS

Target ORF information:

RefSeq Version	NM_018857.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus mesothelin (Msln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018857.1

ORF Insert Sequence:

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGT 
CTCCCCACAG GCCTCTTTCT TGGCCTCACA TGTGAGGAGG TATCTGACCT GAGCATGGAA 
CAAGCCAAGG GGCTGGCTAT GGCTGTAAGA CAGAAGAACA TTACACTCCG GGGACATCAG 
CTGCGTTGTC TGGCACGTCG CCTTCCTAGG CACCTCACCG ACGAGGAACT GAATGCTCTT 
CCACTGGACC TGCTGCTCTT CCTCAACCCA GCCATGTTTC CAGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTGGATGTAC TCCCACGGAG GTCTCTGGAG 
CGCCAGAGGC TGCTGATGGA GGCTCTGAAG TGCCAGGGCG TGTATGGATT TCAAGTGAGT 
GAGGCAGATG TGCGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGAA ATTTGTGGCC 
AGATCTTCCG AAGTTCTCCT CCCCTGGCTG GCAGGATGCC AAGGACCCCT GGACCAGAGC 
CAGGAAAAGG CAGTCAGGGA GGTTCTGAGG AGTGGAAGAA CCCAATATGG CCCCCCATCG 
AAGTGGTCAG TCTCCACCCT GGATGCCCTG CAGAGCTTGG TAGCAGTGTT GGATGAGTCC 
ATCGTCCAGA GCATCCCCAA GGATGTCAAA GCTGAATGGC TGCAACACAT CTCCAGAGAC 
CCCTCCAGGC TGGGGTCTAA GCTGACCGTC ATACACCCAA GGTTCCGACG GGATGCAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCC TACAAGGTGG ATGAAGACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTAGAT GGCACCATGC TGGCCAGACA AATGGACCTT 
GTGAACGAGA TTCCCTTCAC CTATGAGCAG CTCAGTATCT TTAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC TGAGTCCCTG ATCCAGCAGC TGGGTCACTT CTTCAGATAT 
GTTAGCCCTG AAGACATCCA CCAGTGGAAT GTGACCTCAC CAGACACAGT GAAAACTCTG 
CTCAAAGTCA GCAAAGGACA AAAGATGAAT GCTCAGGCGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCCAGCT GGACGAGGAT ATGGTAAAAG CCCTGGGCGA CATCCCGTTA 
AGCTATCTAT GTGACTTCAG CCCCCAGGAT CTGCACTCGG TACCCTCCAG TGTCATGTGG 
CTGGTTGGGC CCCAAGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTCT CCTCTACCAG 
AAGGCCTGCT CAGCCTTCCA GAATGTGAGC GGCCTAGAAT ACTTTGAGAA AATCAAGACA 
TTCCTGGGTG GGGCCTCCGT GAAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGCATG 
GACATAGCCA CTTTCAAGAG GCTGCAGGTG GATTCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTGGGGCC AAACATTGTG GACCTGAAGA CCGAGGAGGA TAAAAGCCCT 
GTCCGTGACT GGCTGTTCCG GCAGCATCAG AAAGACCTAG ACAGGCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGGTC CTGGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGCAGAGCCT CACTCCTTGG GCCAGGATTT GTATTAATAT GGATTCCAGC TCTGCTCCCA 
GCTTTAAGGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msln ( NM_001356286.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343215.1 Mus musculus mesothelin (Msln), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11132
Clone ID Related Accession (Same CDS sequence)	XM_006524650.3 , NM_018857.1 , NM_001356286.1 , NM_018857.2
Accession Version	NM_001356286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	mesothelin isoform 1 preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK144391.1 and D86370.1. On Sep 28, 2017 this sequence version replaced XM_006524650.3. Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK144391.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGT 
CTCCCCACAG GCCTCTTTCT TGGCCTCACA TGTGAGGAGG TATCTGACCT GAGCATGGAA 
CAAGCCAAGG GGCTGGCTAT GGCTGTAAGA CAGAAGAACA TTACACTCCG GGGACATCAG 
CTGCGTTGTC TGGCACGTCG CCTTCCTAGG CACCTCACCG ACGAGGAACT GAATGCTCTT 
CCACTGGACC TGCTGCTCTT CCTCAACCCA GCCATGTTTC CAGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTGGATGTAC TCCCACGGAG GTCTCTGGAG 
CGCCAGAGGC TGCTGATGGA GGCTCTGAAG TGCCAGGGCG TGTATGGATT TCAAGTGAGT 
GAGGCAGATG TGCGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGAA ATTTGTGGCC 
AGATCTTCCG AAGTTCTCCT CCCCTGGCTG GCAGGATGCC AAGGACCCCT GGACCAGAGC 
CAGGAAAAGG CAGTCAGGGA GGTTCTGAGG AGTGGAAGAA CCCAATATGG CCCCCCATCG 
AAGTGGTCAG TCTCCACCCT GGATGCCCTG CAGAGCTTGG TAGCAGTGTT GGATGAGTCC 
ATCGTCCAGA GCATCCCCAA GGATGTCAAA GCTGAATGGC TGCAACACAT CTCCAGAGAC 
CCCTCCAGGC TGGGGTCTAA GCTGACCGTC ATACACCCAA GGTTCCGACG GGATGCAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCC TACAAGGTGG ATGAAGACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTAGAT GGCACCATGC TGGCCAGACA AATGGACCTT 
GTGAACGAGA TTCCCTTCAC CTATGAGCAG CTCAGTATCT TTAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC TGAGTCCCTG ATCCAGCAGC TGGGTCACTT CTTCAGATAT 
GTTAGCCCTG AAGACATCCA CCAGTGGAAT GTGACCTCAC CAGACACAGT GAAAACTCTG 
CTCAAAGTCA GCAAAGGACA AAAGATGAAT GCTCAGGCGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCCAGCT GGACGAGGAT ATGGTAAAAG CCCTGGGCGA CATCCCGTTA 
AGCTATCTAT GTGACTTCAG CCCCCAGGAT CTGCACTCGG TACCCTCCAG TGTCATGTGG 
CTGGTTGGGC CCCAAGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTCT CCTCTACCAG 
AAGGCCTGCT CAGCCTTCCA GAATGTGAGC GGCCTAGAAT ACTTTGAGAA AATCAAGACA 
TTCCTGGGTG GGGCCTCCGT GAAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGCATG 
GACATAGCCA CTTTCAAGAG GCTGCAGGTG GATTCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTGGGGCC AAACATTGTG GACCTGAAGA CCGAGGAGGA TAAAAGCCCT 
GTCCGTGACT GGCTGTTCCG GCAGCATCAG AAAGACCTAG ACAGGCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGGTC CTGGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGCAGAGCCT CACTCCTTGG GCCAGGATTT GTATTAATAT GGATTCCAGC TCTGCTCCCA 
GCTTTAAGGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343215.1
CDS	40..1917
Translation	MALPTARPLLGSCGSPICSRSFLLLLLSLGWIPRLQTQTTKTSQEATLLHAVNGAADFASLPTGLFLGLTCEEVSDLSMEQAKGLAMAVRQKNITLRGHQLRCLARRLPRHLTDEELNALPLDLLLFLNPAMFPGQQACAHFFSLISKANVDVLPRRSLERQRLLMEALKCQGVYGFQVSEADVRALGGLACDLPGKFVARSSEVLLPWLAGCQGPLDQSQEKAVREVLRSGRTQYGPPSKWSVSTLDALQSLVAVLDESIVQSIPKDVKAEWLQHISRDPSRLGSKLTVIHPRFRRDAEQKACPPGKEPYKVDEDLIFYQNWELEACVDGTMLARQMDLVNEIPFTYEQLSIFKHKLDKTYPQGYPESLIQQLGHFFRYVSPEDIHQWNVTSPDTVKTLLKVSKGQKMNAQAIALVACYLRGGGQLDEDMVKALGDIPLSYLCDFSPQDLHSVPSSVMWLVGPQDLDKCSQRHLGLLYQKACSAFQNVSGLEYFEKIKTFLGGASVKDLRALSQHNVSMDIATFKRLQVDSLVGLSVAEVQKLLGPNIVDLKTEEDKSPVRDWLFRQHQKDLDRLGLGLQGGIPNGYLVLDFNVREAFSSRASLLGPGFVLIWIPALLPALRLS

Target ORF information:

RefSeq Version	NM_001356286.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mesothelin (Msln), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356286.1

ORF Insert Sequence:

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGT 
CTCCCCACAG GCCTCTTTCT TGGCCTCACA TGTGAGGAGG TATCTGACCT GAGCATGGAA 
CAAGCCAAGG GGCTGGCTAT GGCTGTAAGA CAGAAGAACA TTACACTCCG GGGACATCAG 
CTGCGTTGTC TGGCACGTCG CCTTCCTAGG CACCTCACCG ACGAGGAACT GAATGCTCTT 
CCACTGGACC TGCTGCTCTT CCTCAACCCA GCCATGTTTC CAGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTGGATGTAC TCCCACGGAG GTCTCTGGAG 
CGCCAGAGGC TGCTGATGGA GGCTCTGAAG TGCCAGGGCG TGTATGGATT TCAAGTGAGT 
GAGGCAGATG TGCGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGAA ATTTGTGGCC 
AGATCTTCCG AAGTTCTCCT CCCCTGGCTG GCAGGATGCC AAGGACCCCT GGACCAGAGC 
CAGGAAAAGG CAGTCAGGGA GGTTCTGAGG AGTGGAAGAA CCCAATATGG CCCCCCATCG 
AAGTGGTCAG TCTCCACCCT GGATGCCCTG CAGAGCTTGG TAGCAGTGTT GGATGAGTCC 
ATCGTCCAGA GCATCCCCAA GGATGTCAAA GCTGAATGGC TGCAACACAT CTCCAGAGAC 
CCCTCCAGGC TGGGGTCTAA GCTGACCGTC ATACACCCAA GGTTCCGACG GGATGCAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCC TACAAGGTGG ATGAAGACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTAGAT GGCACCATGC TGGCCAGACA AATGGACCTT 
GTGAACGAGA TTCCCTTCAC CTATGAGCAG CTCAGTATCT TTAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC TGAGTCCCTG ATCCAGCAGC TGGGTCACTT CTTCAGATAT 
GTTAGCCCTG AAGACATCCA CCAGTGGAAT GTGACCTCAC CAGACACAGT GAAAACTCTG 
CTCAAAGTCA GCAAAGGACA AAAGATGAAT GCTCAGGCGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCCAGCT GGACGAGGAT ATGGTAAAAG CCCTGGGCGA CATCCCGTTA 
AGCTATCTAT GTGACTTCAG CCCCCAGGAT CTGCACTCGG TACCCTCCAG TGTCATGTGG 
CTGGTTGGGC CCCAAGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTCT CCTCTACCAG 
AAGGCCTGCT CAGCCTTCCA GAATGTGAGC GGCCTAGAAT ACTTTGAGAA AATCAAGACA 
TTCCTGGGTG GGGCCTCCGT GAAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGCATG 
GACATAGCCA CTTTCAAGAG GCTGCAGGTG GATTCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTGGGGCC AAACATTGTG GACCTGAAGA CCGAGGAGGA TAAAAGCCCT 
GTCCGTGACT GGCTGTTCCG GCAGCATCAG AAAGACCTAG ACAGGCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGGTC CTGGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGCAGAGCCT CACTCCTTGG GCCAGGATTT GTATTAATAT GGATTCCAGC TCTGCTCCCA 
GCTTTAAGGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msln ( NM_018857.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061345.1 Mus musculus mesothelin (Msln), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11132
Clone ID Related Accession (Same CDS sequence)	XM_006524650.3 , NM_018857.1 , NM_001356286.1 , NM_018857.2
Accession Version	NM_018857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	mesothelin isoform 1 preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK144391.1, AK146799.1 and D86370.1. On Sep 28, 2017 this sequence version replaced NM_018857.1. Transcript Variant: This variant (2) retains an intron in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86370.1, AK146799.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGT 
CTCCCCACAG GCCTCTTTCT TGGCCTCACA TGTGAGGAGG TATCTGACCT GAGCATGGAA 
CAAGCCAAGG GGCTGGCTAT GGCTGTAAGA CAGAAGAACA TTACACTCCG GGGACATCAG 
CTGCGTTGTC TGGCACGTCG CCTTCCTAGG CACCTCACCG ACGAGGAACT GAATGCTCTT 
CCACTGGACC TGCTGCTCTT CCTCAACCCA GCCATGTTTC CAGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTGGATGTAC TCCCACGGAG GTCTCTGGAG 
CGCCAGAGGC TGCTGATGGA GGCTCTGAAG TGCCAGGGCG TGTATGGATT TCAAGTGAGT 
GAGGCAGATG TGCGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGAA ATTTGTGGCC 
AGATCTTCCG AAGTTCTCCT CCCCTGGCTG GCAGGATGCC AAGGACCCCT GGACCAGAGC 
CAGGAAAAGG CAGTCAGGGA GGTTCTGAGG AGTGGAAGAA CCCAATATGG CCCCCCATCG 
AAGTGGTCAG TCTCCACCCT GGATGCCCTG CAGAGCTTGG TAGCAGTGTT GGATGAGTCC 
ATCGTCCAGA GCATCCCCAA GGATGTCAAA GCTGAATGGC TGCAACACAT CTCCAGAGAC 
CCCTCCAGGC TGGGGTCTAA GCTGACCGTC ATACACCCAA GGTTCCGACG GGATGCAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCC TACAAGGTGG ATGAAGACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTAGAT GGCACCATGC TGGCCAGACA AATGGACCTT 
GTGAACGAGA TTCCCTTCAC CTATGAGCAG CTCAGTATCT TTAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC TGAGTCCCTG ATCCAGCAGC TGGGTCACTT CTTCAGATAT 
GTTAGCCCTG AAGACATCCA CCAGTGGAAT GTGACCTCAC CAGACACAGT GAAAACTCTG 
CTCAAAGTCA GCAAAGGACA AAAGATGAAT GCTCAGGCGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCCAGCT GGACGAGGAT ATGGTAAAAG CCCTGGGCGA CATCCCGTTA 
AGCTATCTAT GTGACTTCAG CCCCCAGGAT CTGCACTCGG TACCCTCCAG TGTCATGTGG 
CTGGTTGGGC CCCAAGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTCT CCTCTACCAG 
AAGGCCTGCT CAGCCTTCCA GAATGTGAGC GGCCTAGAAT ACTTTGAGAA AATCAAGACA 
TTCCTGGGTG GGGCCTCCGT GAAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGCATG 
GACATAGCCA CTTTCAAGAG GCTGCAGGTG GATTCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTGGGGCC AAACATTGTG GACCTGAAGA CCGAGGAGGA TAAAAGCCCT 
GTCCGTGACT GGCTGTTCCG GCAGCATCAG AAAGACCTAG ACAGGCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGGTC CTGGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGCAGAGCCT CACTCCTTGG GCCAGGATTT GTATTAATAT GGATTCCAGC TCTGCTCCCA 
GCTTTAAGGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061345.1
CDS	160..2037
Translation	MALPTARPLLGSCGSPICSRSFLLLLLSLGWIPRLQTQTTKTSQEATLLHAVNGAADFASLPTGLFLGLTCEEVSDLSMEQAKGLAMAVRQKNITLRGHQLRCLARRLPRHLTDEELNALPLDLLLFLNPAMFPGQQACAHFFSLISKANVDVLPRRSLERQRLLMEALKCQGVYGFQVSEADVRALGGLACDLPGKFVARSSEVLLPWLAGCQGPLDQSQEKAVREVLRSGRTQYGPPSKWSVSTLDALQSLVAVLDESIVQSIPKDVKAEWLQHISRDPSRLGSKLTVIHPRFRRDAEQKACPPGKEPYKVDEDLIFYQNWELEACVDGTMLARQMDLVNEIPFTYEQLSIFKHKLDKTYPQGYPESLIQQLGHFFRYVSPEDIHQWNVTSPDTVKTLLKVSKGQKMNAQAIALVACYLRGGGQLDEDMVKALGDIPLSYLCDFSPQDLHSVPSSVMWLVGPQDLDKCSQRHLGLLYQKACSAFQNVSGLEYFEKIKTFLGGASVKDLRALSQHNVSMDIATFKRLQVDSLVGLSVAEVQKLLGPNIVDLKTEEDKSPVRDWLFRQHQKDLDRLGLGLQGGIPNGYLVLDFNVREAFSSRASLLGPGFVLIWIPALLPALRLS

Target ORF information:

RefSeq Version	NM_018857.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mesothelin (Msln), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018857.2

ORF Insert Sequence:

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGT 
CTCCCCACAG GCCTCTTTCT TGGCCTCACA TGTGAGGAGG TATCTGACCT GAGCATGGAA 
CAAGCCAAGG GGCTGGCTAT GGCTGTAAGA CAGAAGAACA TTACACTCCG GGGACATCAG 
CTGCGTTGTC TGGCACGTCG CCTTCCTAGG CACCTCACCG ACGAGGAACT GAATGCTCTT 
CCACTGGACC TGCTGCTCTT CCTCAACCCA GCCATGTTTC CAGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTGGATGTAC TCCCACGGAG GTCTCTGGAG 
CGCCAGAGGC TGCTGATGGA GGCTCTGAAG TGCCAGGGCG TGTATGGATT TCAAGTGAGT 
GAGGCAGATG TGCGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGAA ATTTGTGGCC 
AGATCTTCCG AAGTTCTCCT CCCCTGGCTG GCAGGATGCC AAGGACCCCT GGACCAGAGC 
CAGGAAAAGG CAGTCAGGGA GGTTCTGAGG AGTGGAAGAA CCCAATATGG CCCCCCATCG 
AAGTGGTCAG TCTCCACCCT GGATGCCCTG CAGAGCTTGG TAGCAGTGTT GGATGAGTCC 
ATCGTCCAGA GCATCCCCAA GGATGTCAAA GCTGAATGGC TGCAACACAT CTCCAGAGAC 
CCCTCCAGGC TGGGGTCTAA GCTGACCGTC ATACACCCAA GGTTCCGACG GGATGCAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCC TACAAGGTGG ATGAAGACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTAGAT GGCACCATGC TGGCCAGACA AATGGACCTT 
GTGAACGAGA TTCCCTTCAC CTATGAGCAG CTCAGTATCT TTAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC TGAGTCCCTG ATCCAGCAGC TGGGTCACTT CTTCAGATAT 
GTTAGCCCTG AAGACATCCA CCAGTGGAAT GTGACCTCAC CAGACACAGT GAAAACTCTG 
CTCAAAGTCA GCAAAGGACA AAAGATGAAT GCTCAGGCGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCCAGCT GGACGAGGAT ATGGTAAAAG CCCTGGGCGA CATCCCGTTA 
AGCTATCTAT GTGACTTCAG CCCCCAGGAT CTGCACTCGG TACCCTCCAG TGTCATGTGG 
CTGGTTGGGC CCCAAGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTCT CCTCTACCAG 
AAGGCCTGCT CAGCCTTCCA GAATGTGAGC GGCCTAGAAT ACTTTGAGAA AATCAAGACA 
TTCCTGGGTG GGGCCTCCGT GAAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGCATG 
GACATAGCCA CTTTCAAGAG GCTGCAGGTG GATTCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTGGGGCC AAACATTGTG GACCTGAAGA CCGAGGAGGA TAAAAGCCCT 
GTCCGTGACT GGCTGTTCCG GCAGCATCAG AAAGACCTAG ACAGGCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGGTC CTGGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGCAGAGCCT CACTCCTTGG GCCAGGATTT GTATTAATAT GGATTCCAGC TCTGCTCCCA 
GCTTTAAGGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msln ( XM_006524650.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524713.1 Mus musculus mesothelin (Msln), transcript variant X2, mRNA.

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CloneID	OMu11132
Clone ID Related Accession (Same CDS sequence)	XM_006524650.3 , NM_018857.1 , NM_001356286.1 , NM_018857.2
Accession Version	XM_006524650.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	mesothelin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524650.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGT 
CTCCCCACAG GCCTCTTTCT TGGCCTCACA TGTGAGGAGG TATCTGACCT GAGCATGGAA 
CAAGCCAAGG GGCTGGCTAT GGCTGTAAGA CAGAAGAACA TTACACTCCG GGGACATCAG 
CTGCGTTGTC TGGCACGTCG CCTTCCTAGG CACCTCACCG ACGAGGAACT GAATGCTCTT 
CCACTGGACC TGCTGCTCTT CCTCAACCCA GCCATGTTTC CAGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTGGATGTAC TCCCACGGAG GTCTCTGGAG 
CGCCAGAGGC TGCTGATGGA GGCTCTGAAG TGCCAGGGCG TGTATGGATT TCAAGTGAGT 
GAGGCAGATG TGCGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGAA ATTTGTGGCC 
AGATCTTCCG AAGTTCTCCT CCCCTGGCTG GCAGGATGCC AAGGACCCCT GGACCAGAGC 
CAGGAAAAGG CAGTCAGGGA GGTTCTGAGG AGTGGAAGAA CCCAATATGG CCCCCCATCG 
AAGTGGTCAG TCTCCACCCT GGATGCCCTG CAGAGCTTGG TAGCAGTGTT GGATGAGTCC 
ATCGTCCAGA GCATCCCCAA GGATGTCAAA GCTGAATGGC TGCAACACAT CTCCAGAGAC 
CCCTCCAGGC TGGGGTCTAA GCTGACCGTC ATACACCCAA GGTTCCGACG GGATGCAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCC TACAAGGTGG ATGAAGACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTAGAT GGCACCATGC TGGCCAGACA AATGGACCTT 
GTGAACGAGA TTCCCTTCAC CTATGAGCAG CTCAGTATCT TTAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC TGAGTCCCTG ATCCAGCAGC TGGGTCACTT CTTCAGATAT 
GTTAGCCCTG AAGACATCCA CCAGTGGAAT GTGACCTCAC CAGACACAGT GAAAACTCTG 
CTCAAAGTCA GCAAAGGACA AAAGATGAAT GCTCAGGCGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCCAGCT GGACGAGGAT ATGGTAAAAG CCCTGGGCGA CATCCCGTTA 
AGCTATCTAT GTGACTTCAG CCCCCAGGAT CTGCACTCGG TACCCTCCAG TGTCATGTGG 
CTGGTTGGGC CCCAAGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTCT CCTCTACCAG 
AAGGCCTGCT CAGCCTTCCA GAATGTGAGC GGCCTAGAAT ACTTTGAGAA AATCAAGACA 
TTCCTGGGTG GGGCCTCCGT GAAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGCATG 
GACATAGCCA CTTTCAAGAG GCTGCAGGTG GATTCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTGGGGCC AAACATTGTG GACCTGAAGA CCGAGGAGGA TAAAAGCCCT 
GTCCGTGACT GGCTGTTCCG GCAGCATCAG AAAGACCTAG ACAGGCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGGTC CTGGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGCAGAGCCT CACTCCTTGG GCCAGGATTT GTATTAATAT GGATTCCAGC TCTGCTCCCA 
GCTTTAAGGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524713.1
CDS	108..1985
Translation	MALPTARPLLGSCGSPICSRSFLLLLLSLGWIPRLQTQTTKTSQEATLLHAVNGAADFASLPTGLFLGLTCEEVSDLSMEQAKGLAMAVRQKNITLRGHQLRCLARRLPRHLTDEELNALPLDLLLFLNPAMFPGQQACAHFFSLISKANVDVLPRRSLERQRLLMEALKCQGVYGFQVSEADVRALGGLACDLPGKFVARSSEVLLPWLAGCQGPLDQSQEKAVREVLRSGRTQYGPPSKWSVSTLDALQSLVAVLDESIVQSIPKDVKAEWLQHISRDPSRLGSKLTVIHPRFRRDAEQKACPPGKEPYKVDEDLIFYQNWELEACVDGTMLARQMDLVNEIPFTYEQLSIFKHKLDKTYPQGYPESLIQQLGHFFRYVSPEDIHQWNVTSPDTVKTLLKVSKGQKMNAQAIALVACYLRGGGQLDEDMVKALGDIPLSYLCDFSPQDLHSVPSSVMWLVGPQDLDKCSQRHLGLLYQKACSAFQNVSGLEYFEKIKTFLGGASVKDLRALSQHNVSMDIATFKRLQVDSLVGLSVAEVQKLLGPNIVDLKTEEDKSPVRDWLFRQHQKDLDRLGLGLQGGIPNGYLVLDFNVREAFSSRASLLGPGFVLIWIPALLPALRLS

Target ORF information:

RefSeq Version	XM_006524650.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus mesothelin (Msln), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524650.3

ORF Insert Sequence:

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGT 
CTCCCCACAG GCCTCTTTCT TGGCCTCACA TGTGAGGAGG TATCTGACCT GAGCATGGAA 
CAAGCCAAGG GGCTGGCTAT GGCTGTAAGA CAGAAGAACA TTACACTCCG GGGACATCAG 
CTGCGTTGTC TGGCACGTCG CCTTCCTAGG CACCTCACCG ACGAGGAACT GAATGCTCTT 
CCACTGGACC TGCTGCTCTT CCTCAACCCA GCCATGTTTC CAGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTGGATGTAC TCCCACGGAG GTCTCTGGAG 
CGCCAGAGGC TGCTGATGGA GGCTCTGAAG TGCCAGGGCG TGTATGGATT TCAAGTGAGT 
GAGGCAGATG TGCGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGAA ATTTGTGGCC 
AGATCTTCCG AAGTTCTCCT CCCCTGGCTG GCAGGATGCC AAGGACCCCT GGACCAGAGC 
CAGGAAAAGG CAGTCAGGGA GGTTCTGAGG AGTGGAAGAA CCCAATATGG CCCCCCATCG 
AAGTGGTCAG TCTCCACCCT GGATGCCCTG CAGAGCTTGG TAGCAGTGTT GGATGAGTCC 
ATCGTCCAGA GCATCCCCAA GGATGTCAAA GCTGAATGGC TGCAACACAT CTCCAGAGAC 
CCCTCCAGGC TGGGGTCTAA GCTGACCGTC ATACACCCAA GGTTCCGACG GGATGCAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCC TACAAGGTGG ATGAAGACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTAGAT GGCACCATGC TGGCCAGACA AATGGACCTT 
GTGAACGAGA TTCCCTTCAC CTATGAGCAG CTCAGTATCT TTAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC TGAGTCCCTG ATCCAGCAGC TGGGTCACTT CTTCAGATAT 
GTTAGCCCTG AAGACATCCA CCAGTGGAAT GTGACCTCAC CAGACACAGT GAAAACTCTG 
CTCAAAGTCA GCAAAGGACA AAAGATGAAT GCTCAGGCGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCCAGCT GGACGAGGAT ATGGTAAAAG CCCTGGGCGA CATCCCGTTA 
AGCTATCTAT GTGACTTCAG CCCCCAGGAT CTGCACTCGG TACCCTCCAG TGTCATGTGG 
CTGGTTGGGC CCCAAGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTCT CCTCTACCAG 
AAGGCCTGCT CAGCCTTCCA GAATGTGAGC GGCCTAGAAT ACTTTGAGAA AATCAAGACA 
TTCCTGGGTG GGGCCTCCGT GAAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGCATG 
GACATAGCCA CTTTCAAGAG GCTGCAGGTG GATTCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTGGGGCC AAACATTGTG GACCTGAAGA CCGAGGAGGA TAAAAGCCCT 
GTCCGTGACT GGCTGTTCCG GCAGCATCAG AAAGACCTAG ACAGGCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGGTC CTGGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGCAGAGCCT CACTCCTTGG GCCAGGATTT GTATTAATAT GGATTCCAGC TCTGCTCCCA 
GCTTTAAGGC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msln ( XM_006524649.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524712.1 Mus musculus mesothelin (Msln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53845
Clone ID Related Accession (Same CDS sequence)	XM_006524649.3 , NM_001374653.1
Accession Version	XM_006524649.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mesothelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGC 
CCTGTCCCCA GTCTCCCCAC AGGCCTCTTT CTTGGCCTCA CATGTGAGGA GGTATCTGAC 
CTGAGCATGG AACAAGCCAA GGGGCTGGCT ATGGCTGTAA GACAGAAGAA CATTACACTC 
CGGGGACATC AGCTGCGTTG TCTGGCACGT CGCCTTCCTA GGCACCTCAC CGACGAGGAA 
CTGAATGCTC TTCCACTGGA CCTGCTGCTC TTCCTCAACC CAGCCATGTT TCCAGGGCAA 
CAGGCTTGTG CCCACTTCTT CTCCCTCATC TCTAAAGCCA ATGTGGATGT ACTCCCACGG 
AGGTCTCTGG AGCGCCAGAG GCTGCTGATG GAGGCTCTGA AGTGCCAGGG CGTGTATGGA 
TTTCAAGTGA GTGAGGCAGA TGTGCGGGCT CTCGGAGGCC TGGCCTGTGA CCTGCCTGGG 
AAATTTGTGG CCAGATCTTC CGAAGTTCTC CTCCCCTGGC TGGCAGGATG CCAAGGACCC 
CTGGACCAGA GCCAGGAAAA GGCAGTCAGG GAGGTTCTGA GGAGTGGAAG AACCCAATAT 
GGCCCCCCAT CGAAGTGGTC AGTCTCCACC CTGGATGCCC TGCAGAGCTT GGTAGCAGTG 
TTGGATGAGT CCATCGTCCA GAGCATCCCC AAGGATGTCA AAGCTGAATG GCTGCAACAC 
ATCTCCAGAG ACCCCTCCAG GCTGGGGTCT AAGCTGACCG TCATACACCC AAGGTTCCGA 
CGGGATGCAG AACAGAAAGC CTGCCCTCCA GGGAAGGAGC CCTACAAGGT GGATGAAGAC 
CTCATCTTCT ACCAGAATTG GGAGCTGGAG GCTTGTGTAG ATGGCACCAT GCTGGCCAGA 
CAAATGGACC TTGTGAACGA GATTCCCTTC ACCTATGAGC AGCTCAGTAT CTTTAAGCAC 
AAACTGGACA AGACCTACCC ACAAGGCTAT CCTGAGTCCC TGATCCAGCA GCTGGGTCAC 
TTCTTCAGAT ATGTTAGCCC TGAAGACATC CACCAGTGGA ATGTGACCTC ACCAGACACA 
GTGAAAACTC TGCTCAAAGT CAGCAAAGGA CAAAAGATGA ATGCTCAGGC GATTGCCTTG 
GTCGCCTGCT ATCTTCGGGG AGGAGGCCAG CTGGACGAGG ATATGGTAAA AGCCCTGGGC 
GACATCCCGT TAAGCTATCT ATGTGACTTC AGCCCCCAGG ATCTGCACTC GGTACCCTCC 
AGTGTCATGT GGCTGGTTGG GCCCCAAGAC CTGGACAAGT GCAGCCAGAG GCATCTGGGT 
CTCCTCTACC AGAAGGCCTG CTCAGCCTTC CAGAATGTGA GCGGCCTAGA ATACTTTGAG 
AAAATCAAGA CATTCCTGGG TGGGGCCTCC GTGAAGGACC TGCGGGCCCT CAGCCAGCAC 
AATGTGAGCA TGGACATAGC CACTTTCAAG AGGCTGCAGG TGGATTCCCT GGTGGGGCTG 
AGTGTGGCTG AGGTACAGAA ACTTCTGGGG CCAAACATTG TGGACCTGAA GACCGAGGAG 
GATAAAAGCC CTGTCCGTGA CTGGCTGTTC CGGCAGCATC AGAAAGACCT AGACAGGCTG 
GGTTTGGGAC TTCAGGGTGG CATCCCCAAT GGCTACCTGG TCCTGGACTT CAATGTCCGA 
GAGGCCTTCT CCAGCAGAGC CTCACTCCTT GGGCCAGGAT TTGTATTAAT ATGGATTCCA 
GCTCTGCTCC CAGCTTTAAG GCTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524712.1
CDS	108..1997
Translation	MALPTARPLLGSCGSPICSRSFLLLLLSLGWIPRLQTQTTKTSQEATLLHAVNGAADFASPVPSLPTGLFLGLTCEEVSDLSMEQAKGLAMAVRQKNITLRGHQLRCLARRLPRHLTDEELNALPLDLLLFLNPAMFPGQQACAHFFSLISKANVDVLPRRSLERQRLLMEALKCQGVYGFQVSEADVRALGGLACDLPGKFVARSSEVLLPWLAGCQGPLDQSQEKAVREVLRSGRTQYGPPSKWSVSTLDALQSLVAVLDESIVQSIPKDVKAEWLQHISRDPSRLGSKLTVIHPRFRRDAEQKACPPGKEPYKVDEDLIFYQNWELEACVDGTMLARQMDLVNEIPFTYEQLSIFKHKLDKTYPQGYPESLIQQLGHFFRYVSPEDIHQWNVTSPDTVKTLLKVSKGQKMNAQAIALVACYLRGGGQLDEDMVKALGDIPLSYLCDFSPQDLHSVPSSVMWLVGPQDLDKCSQRHLGLLYQKACSAFQNVSGLEYFEKIKTFLGGASVKDLRALSQHNVSMDIATFKRLQVDSLVGLSVAEVQKLLGPNIVDLKTEEDKSPVRDWLFRQHQKDLDRLGLGLQGGIPNGYLVLDFNVREAFSSRASLLGPGFVLIWIPALLPALRLS

Target ORF information:

RefSeq Version	XM_006524649.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mesothelin (Msln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524649.3

ORF Insert Sequence:

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGC 
CCTGTCCCCA GTCTCCCCAC AGGCCTCTTT CTTGGCCTCA CATGTGAGGA GGTATCTGAC 
CTGAGCATGG AACAAGCCAA GGGGCTGGCT ATGGCTGTAA GACAGAAGAA CATTACACTC 
CGGGGACATC AGCTGCGTTG TCTGGCACGT CGCCTTCCTA GGCACCTCAC CGACGAGGAA 
CTGAATGCTC TTCCACTGGA CCTGCTGCTC TTCCTCAACC CAGCCATGTT TCCAGGGCAA 
CAGGCTTGTG CCCACTTCTT CTCCCTCATC TCTAAAGCCA ATGTGGATGT ACTCCCACGG 
AGGTCTCTGG AGCGCCAGAG GCTGCTGATG GAGGCTCTGA AGTGCCAGGG CGTGTATGGA 
TTTCAAGTGA GTGAGGCAGA TGTGCGGGCT CTCGGAGGCC TGGCCTGTGA CCTGCCTGGG 
AAATTTGTGG CCAGATCTTC CGAAGTTCTC CTCCCCTGGC TGGCAGGATG CCAAGGACCC 
CTGGACCAGA GCCAGGAAAA GGCAGTCAGG GAGGTTCTGA GGAGTGGAAG AACCCAATAT 
GGCCCCCCAT CGAAGTGGTC AGTCTCCACC CTGGATGCCC TGCAGAGCTT GGTAGCAGTG 
TTGGATGAGT CCATCGTCCA GAGCATCCCC AAGGATGTCA AAGCTGAATG GCTGCAACAC 
ATCTCCAGAG ACCCCTCCAG GCTGGGGTCT AAGCTGACCG TCATACACCC AAGGTTCCGA 
CGGGATGCAG AACAGAAAGC CTGCCCTCCA GGGAAGGAGC CCTACAAGGT GGATGAAGAC 
CTCATCTTCT ACCAGAATTG GGAGCTGGAG GCTTGTGTAG ATGGCACCAT GCTGGCCAGA 
CAAATGGACC TTGTGAACGA GATTCCCTTC ACCTATGAGC AGCTCAGTAT CTTTAAGCAC 
AAACTGGACA AGACCTACCC ACAAGGCTAT CCTGAGTCCC TGATCCAGCA GCTGGGTCAC 
TTCTTCAGAT ATGTTAGCCC TGAAGACATC CACCAGTGGA ATGTGACCTC ACCAGACACA 
GTGAAAACTC TGCTCAAAGT CAGCAAAGGA CAAAAGATGA ATGCTCAGGC GATTGCCTTG 
GTCGCCTGCT ATCTTCGGGG AGGAGGCCAG CTGGACGAGG ATATGGTAAA AGCCCTGGGC 
GACATCCCGT TAAGCTATCT ATGTGACTTC AGCCCCCAGG ATCTGCACTC GGTACCCTCC 
AGTGTCATGT GGCTGGTTGG GCCCCAAGAC CTGGACAAGT GCAGCCAGAG GCATCTGGGT 
CTCCTCTACC AGAAGGCCTG CTCAGCCTTC CAGAATGTGA GCGGCCTAGA ATACTTTGAG 
AAAATCAAGA CATTCCTGGG TGGGGCCTCC GTGAAGGACC TGCGGGCCCT CAGCCAGCAC 
AATGTGAGCA TGGACATAGC CACTTTCAAG AGGCTGCAGG TGGATTCCCT GGTGGGGCTG 
AGTGTGGCTG AGGTACAGAA ACTTCTGGGG CCAAACATTG TGGACCTGAA GACCGAGGAG 
GATAAAAGCC CTGTCCGTGA CTGGCTGTTC CGGCAGCATC AGAAAGACCT AGACAGGCTG 
GGTTTGGGAC TTCAGGGTGG CATCCCCAAT GGCTACCTGG TCCTGGACTT CAATGTCCGA 
GAGGCCTTCT CCAGCAGAGC CTCACTCCTT GGGCCAGGAT TTGTATTAAT ATGGATTCCA 
GCTCTGCTCC CAGCTTTAAG GCTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msln ( NM_001374653.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001361582.1 Mus musculus mesothelin (Msln), transcript variant 3, mRNA.

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CloneID	OMu53845
Clone ID Related Accession (Same CDS sequence)	XM_006524649.3 , NM_001374653.1
Accession Version	NM_001374653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Mus musculus(house mouse)
Product	mesothelin isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC134908.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGC 
CCTGTCCCCA GTCTCCCCAC AGGCCTCTTT CTTGGCCTCA CATGTGAGGA GGTATCTGAC 
CTGAGCATGG AACAAGCCAA GGGGCTGGCT ATGGCTGTAA GACAGAAGAA CATTACACTC 
CGGGGACATC AGCTGCGTTG TCTGGCACGT CGCCTTCCTA GGCACCTCAC CGACGAGGAA 
CTGAATGCTC TTCCACTGGA CCTGCTGCTC TTCCTCAACC CAGCCATGTT TCCAGGGCAA 
CAGGCTTGTG CCCACTTCTT CTCCCTCATC TCTAAAGCCA ATGTGGATGT ACTCCCACGG 
AGGTCTCTGG AGCGCCAGAG GCTGCTGATG GAGGCTCTGA AGTGCCAGGG CGTGTATGGA 
TTTCAAGTGA GTGAGGCAGA TGTGCGGGCT CTCGGAGGCC TGGCCTGTGA CCTGCCTGGG 
AAATTTGTGG CCAGATCTTC CGAAGTTCTC CTCCCCTGGC TGGCAGGATG CCAAGGACCC 
CTGGACCAGA GCCAGGAAAA GGCAGTCAGG GAGGTTCTGA GGAGTGGAAG AACCCAATAT 
GGCCCCCCAT CGAAGTGGTC AGTCTCCACC CTGGATGCCC TGCAGAGCTT GGTAGCAGTG 
TTGGATGAGT CCATCGTCCA GAGCATCCCC AAGGATGTCA AAGCTGAATG GCTGCAACAC 
ATCTCCAGAG ACCCCTCCAG GCTGGGGTCT AAGCTGACCG TCATACACCC AAGGTTCCGA 
CGGGATGCAG AACAGAAAGC CTGCCCTCCA GGGAAGGAGC CCTACAAGGT GGATGAAGAC 
CTCATCTTCT ACCAGAATTG GGAGCTGGAG GCTTGTGTAG ATGGCACCAT GCTGGCCAGA 
CAAATGGACC TTGTGAACGA GATTCCCTTC ACCTATGAGC AGCTCAGTAT CTTTAAGCAC 
AAACTGGACA AGACCTACCC ACAAGGCTAT CCTGAGTCCC TGATCCAGCA GCTGGGTCAC 
TTCTTCAGAT ATGTTAGCCC TGAAGACATC CACCAGTGGA ATGTGACCTC ACCAGACACA 
GTGAAAACTC TGCTCAAAGT CAGCAAAGGA CAAAAGATGA ATGCTCAGGC GATTGCCTTG 
GTCGCCTGCT ATCTTCGGGG AGGAGGCCAG CTGGACGAGG ATATGGTAAA AGCCCTGGGC 
GACATCCCGT TAAGCTATCT ATGTGACTTC AGCCCCCAGG ATCTGCACTC GGTACCCTCC 
AGTGTCATGT GGCTGGTTGG GCCCCAAGAC CTGGACAAGT GCAGCCAGAG GCATCTGGGT 
CTCCTCTACC AGAAGGCCTG CTCAGCCTTC CAGAATGTGA GCGGCCTAGA ATACTTTGAG 
AAAATCAAGA CATTCCTGGG TGGGGCCTCC GTGAAGGACC TGCGGGCCCT CAGCCAGCAC 
AATGTGAGCA TGGACATAGC CACTTTCAAG AGGCTGCAGG TGGATTCCCT GGTGGGGCTG 
AGTGTGGCTG AGGTACAGAA ACTTCTGGGG CCAAACATTG TGGACCTGAA GACCGAGGAG 
GATAAAAGCC CTGTCCGTGA CTGGCTGTTC CGGCAGCATC AGAAAGACCT AGACAGGCTG 
GGTTTGGGAC TTCAGGGTGG CATCCCCAAT GGCTACCTGG TCCTGGACTT CAATGTCCGA 
GAGGCCTTCT CCAGCAGAGC CTCACTCCTT GGGCCAGGAT TTGTATTAAT ATGGATTCCA 
GCTCTGCTCC CAGCTTTAAG GCTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361582.1
CDS	14..1903
Translation	MALPTARPLLGSCGSPICSRSFLLLLLSLGWIPRLQTQTTKTSQEATLLHAVNGAADFASPVPSLPTGLFLGLTCEEVSDLSMEQAKGLAMAVRQKNITLRGHQLRCLARRLPRHLTDEELNALPLDLLLFLNPAMFPGQQACAHFFSLISKANVDVLPRRSLERQRLLMEALKCQGVYGFQVSEADVRALGGLACDLPGKFVARSSEVLLPWLAGCQGPLDQSQEKAVREVLRSGRTQYGPPSKWSVSTLDALQSLVAVLDESIVQSIPKDVKAEWLQHISRDPSRLGSKLTVIHPRFRRDAEQKACPPGKEPYKVDEDLIFYQNWELEACVDGTMLARQMDLVNEIPFTYEQLSIFKHKLDKTYPQGYPESLIQQLGHFFRYVSPEDIHQWNVTSPDTVKTLLKVSKGQKMNAQAIALVACYLRGGGQLDEDMVKALGDIPLSYLCDFSPQDLHSVPSSVMWLVGPQDLDKCSQRHLGLLYQKACSAFQNVSGLEYFEKIKTFLGGASVKDLRALSQHNVSMDIATFKRLQVDSLVGLSVAEVQKLLGPNIVDLKTEEDKSPVRDWLFRQHQKDLDRLGLGLQGGIPNGYLVLDFNVREAFSSRASLLGPGFVLIWIPALLPALRLS

Target ORF information:

RefSeq Version	NM_001374653.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mesothelin (Msln), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374653.1

ORF Insert Sequence:

ATGGCCTTGC CAACAGCTCG ACCCCTGCTG GGGTCCTGTG GAAGTCCCAT CTGCAGCCGA 
AGCTTCCTAC TGCTTCTCCT TAGTCTTGGG TGGATACCAC GTCTGCAGAC CCAGACTACA 
AAGACAAGCC AGGAGGCCAC ACTCCTCCAT GCTGTGAACG GTGCCGCTGA CTTTGCCAGC 
CCTGTCCCCA GTCTCCCCAC AGGCCTCTTT CTTGGCCTCA CATGTGAGGA GGTATCTGAC 
CTGAGCATGG AACAAGCCAA GGGGCTGGCT ATGGCTGTAA GACAGAAGAA CATTACACTC 
CGGGGACATC AGCTGCGTTG TCTGGCACGT CGCCTTCCTA GGCACCTCAC CGACGAGGAA 
CTGAATGCTC TTCCACTGGA CCTGCTGCTC TTCCTCAACC CAGCCATGTT TCCAGGGCAA 
CAGGCTTGTG CCCACTTCTT CTCCCTCATC TCTAAAGCCA ATGTGGATGT ACTCCCACGG 
AGGTCTCTGG AGCGCCAGAG GCTGCTGATG GAGGCTCTGA AGTGCCAGGG CGTGTATGGA 
TTTCAAGTGA GTGAGGCAGA TGTGCGGGCT CTCGGAGGCC TGGCCTGTGA CCTGCCTGGG 
AAATTTGTGG CCAGATCTTC CGAAGTTCTC CTCCCCTGGC TGGCAGGATG CCAAGGACCC 
CTGGACCAGA GCCAGGAAAA GGCAGTCAGG GAGGTTCTGA GGAGTGGAAG AACCCAATAT 
GGCCCCCCAT CGAAGTGGTC AGTCTCCACC CTGGATGCCC TGCAGAGCTT GGTAGCAGTG 
TTGGATGAGT CCATCGTCCA GAGCATCCCC AAGGATGTCA AAGCTGAATG GCTGCAACAC 
ATCTCCAGAG ACCCCTCCAG GCTGGGGTCT AAGCTGACCG TCATACACCC AAGGTTCCGA 
CGGGATGCAG AACAGAAAGC CTGCCCTCCA GGGAAGGAGC CCTACAAGGT GGATGAAGAC 
CTCATCTTCT ACCAGAATTG GGAGCTGGAG GCTTGTGTAG ATGGCACCAT GCTGGCCAGA 
CAAATGGACC TTGTGAACGA GATTCCCTTC ACCTATGAGC AGCTCAGTAT CTTTAAGCAC 
AAACTGGACA AGACCTACCC ACAAGGCTAT CCTGAGTCCC TGATCCAGCA GCTGGGTCAC 
TTCTTCAGAT ATGTTAGCCC TGAAGACATC CACCAGTGGA ATGTGACCTC ACCAGACACA 
GTGAAAACTC TGCTCAAAGT CAGCAAAGGA CAAAAGATGA ATGCTCAGGC GATTGCCTTG 
GTCGCCTGCT ATCTTCGGGG AGGAGGCCAG CTGGACGAGG ATATGGTAAA AGCCCTGGGC 
GACATCCCGT TAAGCTATCT ATGTGACTTC AGCCCCCAGG ATCTGCACTC GGTACCCTCC 
AGTGTCATGT GGCTGGTTGG GCCCCAAGAC CTGGACAAGT GCAGCCAGAG GCATCTGGGT 
CTCCTCTACC AGAAGGCCTG CTCAGCCTTC CAGAATGTGA GCGGCCTAGA ATACTTTGAG 
AAAATCAAGA CATTCCTGGG TGGGGCCTCC GTGAAGGACC TGCGGGCCCT CAGCCAGCAC 
AATGTGAGCA TGGACATAGC CACTTTCAAG AGGCTGCAGG TGGATTCCCT GGTGGGGCTG 
AGTGTGGCTG AGGTACAGAA ACTTCTGGGG CCAAACATTG TGGACCTGAA GACCGAGGAG 
GATAAAAGCC CTGTCCGTGA CTGGCTGTTC CGGCAGCATC AGAAAGACCT AGACAGGCTG 
GGTTTGGGAC TTCAGGGTGG CATCCCCAAT GGCTACCTGG TCCTGGACTT CAATGTCCGA 
GAGGCCTTCT CCAGCAGAGC CTCACTCCTT GGGCCAGGAT TTGTATTAAT ATGGATTCCA 
GCTCTGCTCC CAGCTTTAAG GCTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mesothelin/mucin 16 signaling in activated portal fibroblasts regulates cholestatic liver fibrosis.
The Journal of clinical investigation127(4)1254-1270(2017 Apr)
Koyama Y,Wang P,Liang S,Iwaisako K,Liu X,Xu J,Zhang M,Sun M,Cong M,Karin D,Taura K,Benner C,Heinz S,Bera T,Brenner DA,Kisseleva T

Overexpression of sulfatase-1 in murine hepatocarcinoma Hca-F cell line downregulates mesothelin and leads to reduction in lymphatic metastasis, both in vitro and in vivo.
Oncotarget7(46)75052-75063(2016 11)
Mahmoud S,Ibrahim M,Hago A,Huang Y,Wei Y,Zhang J,Zhang Q,Xiao Y,Wang J,Adam M,Guo Y,Wang L,Zhou S,Xin B,Xuan W,Tang J

Murine mesothelin: characterization, expression, and inhibition of tumor growth in a murine model of pancreatic cancer.
Journal of experimental & clinical cancer research : CR3539(2016 Mar)
Zervos E,Agle S,Freistaedter AG,Jones GJ,Roper RL

T Cells Engineered against a Native Antigen Can Surmount Immunologic and Physical Barriers to Treat Pancreatic Ductal Adenocarcinoma.
Cancer cell28(5)638-652(2015 Nov)
Stromnes IM,Schmitt TM,Hulbert A,Brockenbrough JS,Nguyen H,Cuevas C,Dotson AM,Tan X,Hotes JL,Greenberg PD,Hingorani SR

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Msln cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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