Msln cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Msln
Entrez Gene ID	60333
Full Name	mesothelin
General protein information	Preferred Names mesothelin Names mesothelin ERC/mesothelin pre-pro-megakaryocyte-potentiating factor protein expressed in renal carcinoma
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	may play a role in cell adhesion and cell shape dynamics; human homolog highly expressed in renal cell carcinoma [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246022.2	XP_006246084.1	mesothelin isoform X1
NM_031658.1	NP_113846.1	mesothelin precursor
XM_006246021.3	XP_006246083.1	mesothelin isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MSLN	XP_003952796.1
Mus musculus (house mouse)	Msln	NP_061345.1
Xenopus tropicalis (tropical clawed frog)	LOC101733400	XP_004918363.1
Homo sapiens (human)	MSLN	NP_001170826.1
Canis lupus familiaris (dog)	MSLN	XP_854112.1
Bos taurus (cattle)	MSLN	NP_001093844.1
Rattus norvegicus (Norway rat)	Msln	NP_113846.1
Gallus gallus (chicken)	MSLN	XP_414835.3
	MSLN	XP_001087333.2
Xenopus tropicalis (tropical clawed frog)	LOC100489413	XP_002935910.2
Xenopus tropicalis (tropical clawed frog)	LOC101733470	XP_004918364.1

Gene Ontology
Bibliography

Related articles in PubMed

Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas.
Yin DD, You LH, Yuan QX, Liang XD, Wang N, Wang LT, Yuan L, Wang KM, De W
World journal of gastroenterology20(22)6884-96(2014 Jun)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Effectiveness of serum megakaryocyte potentiating factor in evaluating the effects of chrysotile and its heated products on respiratory organs.
Takata A, Yamauchi H, Toya T, Miyamoto-Kohno S, Iwatatsu Y, Teranaka I, Aminaka M, Yamashita K, Kohyama N
Toxicology and applied pharmacology252(2)123-9(2011 Apr)

An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas.
Fukamachi K, Tanaka H, Hagiwara Y, Ohara H, Joh T, Iigo M, Alexander DB, Xu J, Long N, Takigahira M, Yanagihara K, Hino O, Saito I, Tsuda H
Biochemical and biophysical research communications390(3)636-41(2009 Dec)

Circulating mesothelin protein and cellular antimesothelin immunity in patients with pancreatic cancer.
Johnston FM, Tan MC, Tan BR Jr, Porembka MR, Brunt EM, Linehan DC, Simon PO Jr, Plambeck-Suess S, Eberlein TJ, Hellstrom KE, Hellstrom I, Hawkins WG, Goedegebuure P
Clinical cancer research : an official journal of the American Association for Cancer Research15(21)6511-8(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats.
Title: Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas.

Megakaryocyte potentiating factor is a useful serum biomarker for the effects of chrysotile exposure on respiratory organs.
Title: Effectiveness of serum megakaryocyte potentiating factor in evaluating the effects of chrysotile and its heated products on respiratory organs.

Mesothelin might be involved in some developmental events during development of rat pancreas.
Title: Expression and localization of mesothelin in developing rat pancreas.

The following Msln gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Msln cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa32915	XM_006246021.3 Latest version!	Rattus norvegicus mesothelin (Msln), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa32915	XM_006246022.2 Latest version!	Rattus norvegicus mesothelin (Msln), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa08403	NM_031658.1 Latest version!	Rattus norvegicus mesothelin (Msln), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Msln ( XM_006246022.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246084.1 Rattus norvegicus mesothelin (Msln), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa32915
Clone ID Related Accession (Same CDS sequence)	XM_006246022.2 , XM_006246021.3
Accession Version	XM_006246022.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mesothelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGC CAACAGCCCA ACCCCTGCTG GGGTCCTGTG GAAGCCCCAT CTGCAGCCGC 
AGCTTTCTAC TGCTTCTCCT TAGTCTTGGG TGGTTGCCAC TTCTGCAGAC CCAGACTACA 
AGGACAAGCC AGGAGGCCGC ACTTCTCCAT GCTGTGACCG GCACCGTTGA CTTTGCCAGT 
CTTCCCACAG GCCTCTTTCT TGGCCTCATG TGTGATGAGG TATCTGGCCT AAGCATGGGA 
CACGCCAAGG AGCTGGCTAT GGCTGTGAGA CAGAAGAATA TCGTGCTCCA AGTACATCAG 
CTGCGCTGTC TGGCCCGTCG CCTCCCTAAG CACCTCACCA ACGAGGAACT GGATGCTCTC 
CCACTGGACC TGCTGCTCTT CCTCAATCCA GCCATGTTTC CGGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTAAATGTAC TCCCACGGAG ATCTCTGGAG 
CGCCAGAGGC TGCTGACCGG GGCTCTGAAA TGCCAGGGTG TGTATGGATT TCAAGTGAGT 
GAGACGGATG CACGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGGA ATTCGTGGCC 
AAATCTTCGG AAGTCCTCCT CCCCTGGCTG GCAAGATGCG GAGGACCCCT GGACCAAGGC 
CAGGCAAAGG CTGTCAGGGA GGTTCTGAGG AGTGGAAGAG CCCCCTATGG TCCCCCATCG 
ACGTGGTCAG TCTCCACCCT GGATGCCCTG CAGGGTTTGC TGGTAGTGTT GGATGAGTCC 
ATTGTCCACA GCATCCCTAA GGATGTTATC ACTGAATGGC TGCAAGGCAT CTCCAGAGAG 
CCCTCCAGGC TGGGGTCTAA GTGGACTGTC ACACACCCAA GGTTCCGGCG GGACACAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCT AACGTGGTGG ATGAAAACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTCGAT GGTACCCTGC TGGCCGGCCA GATGGACCTT 
GTGAATGAAA TTCCCTTTAC CTACGAGCAG CTCAGCATCT TCAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC CGAGTCCCTG ATCAAGCAGC TGGGCCACTT CTTCAGATAC 
GTTAGCCCTG AGGACATCCG GCAGTGGAAT GTGACTTCAC CAGACACAGT GAATACTCTG 
CTTAAAGTCA GCAAAGGACA AAAGATGGAT GCTCAGGTGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCAAGCT GGATGAGGAC ATAGTAAAAG CCCTGGACAA CATCCCCTTA 
AGTTACCTAT GTGACTTCAG CCCCCAGGAT CTGCACGCTA TACCCTCCAG TGTTATGTGG 
CTGGTTGGGC TCCATGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTAT CCTCTATCAG 
AAGGCCTGCT CAGCCTTCCA GAACGTGAGC GGGCTGGAAT ACTTTGAGAA AATCAGGACA 
TTTCTGGGTG GGGCCTCCAG GGAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGTATG 
GACATAGCCA CTTTCAAGAA GCTGCAGGTG GATGCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTAGGGCC ACACATTGGG GACCTGAAGA CTGAGGAGGA TAAAAGCCCT 
GTCCGGGACT GGCTCTTCCG ACAGCAGCAG AAAGACCTGG ACAGTCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGATC CTAGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGTGGAGCCC CACTCCTTGG GCCAGGATTT GTGTTTGCAT GGATTCCAGC TCTGCTCTCA 
GCTTTAAGAC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246084.1
CDS	49..1926
Translation	MALPTAQPLLGSCGSPICSRSFLLLLLSLGWLPLLQTQTTRTSQEAALLHAVTGTVDFASLPTGLFLGLMCDEVSGLSMGHAKELAMAVRQKNIVLQVHQLRCLARRLPKHLTNEELDALPLDLLLFLNPAMFPGQQACAHFFSLISKANVNVLPRRSLERQRLLTGALKCQGVYGFQVSETDARALGGLACDLPGEFVAKSSEVLLPWLARCGGPLDQGQAKAVREVLRSGRAPYGPPSTWSVSTLDALQGLLVVLDESIVHSIPKDVITEWLQGISREPSRLGSKWTVTHPRFRRDTEQKACPPGKEPNVVDENLIFYQNWELEACVDGTLLAGQMDLVNEIPFTYEQLSIFKHKLDKTYPQGYPESLIKQLGHFFRYVSPEDIRQWNVTSPDTVNTLLKVSKGQKMDAQVIALVACYLRGGGKLDEDIVKALDNIPLSYLCDFSPQDLHAIPSSVMWLVGLHDLDKCSQRHLGILYQKACSAFQNVSGLEYFEKIRTFLGGASREDLRALSQHNVSMDIATFKKLQVDALVGLSVAEVQKLLGPHIGDLKTEEDKSPVRDWLFRQQQKDLDSLGLGLQGGIPNGYLILDFNVREAFSSGAPLLGPGFVFAWIPALLSALRLS

Target ORF information:

RefSeq Version	XM_006246022.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mesothelin (Msln), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246022.2

ORF Insert Sequence:

ATGGCCTTGC CAACAGCCCA ACCCCTGCTG GGGTCCTGTG GAAGCCCCAT CTGCAGCCGC 
AGCTTTCTAC TGCTTCTCCT TAGTCTTGGG TGGTTGCCAC TTCTGCAGAC CCAGACTACA 
AGGACAAGCC AGGAGGCCGC ACTTCTCCAT GCTGTGACCG GCACCGTTGA CTTTGCCAGT 
CTTCCCACAG GCCTCTTTCT TGGCCTCATG TGTGATGAGG TATCTGGCCT AAGCATGGGA 
CACGCCAAGG AGCTGGCTAT GGCTGTGAGA CAGAAGAATA TCGTGCTCCA AGTACATCAG 
CTGCGCTGTC TGGCCCGTCG CCTCCCTAAG CACCTCACCA ACGAGGAACT GGATGCTCTC 
CCACTGGACC TGCTGCTCTT CCTCAATCCA GCCATGTTTC CGGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTAAATGTAC TCCCACGGAG ATCTCTGGAG 
CGCCAGAGGC TGCTGACCGG GGCTCTGAAA TGCCAGGGTG TGTATGGATT TCAAGTGAGT 
GAGACGGATG CACGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGGA ATTCGTGGCC 
AAATCTTCGG AAGTCCTCCT CCCCTGGCTG GCAAGATGCG GAGGACCCCT GGACCAAGGC 
CAGGCAAAGG CTGTCAGGGA GGTTCTGAGG AGTGGAAGAG CCCCCTATGG TCCCCCATCG 
ACGTGGTCAG TCTCCACCCT GGATGCCCTG CAGGGTTTGC TGGTAGTGTT GGATGAGTCC 
ATTGTCCACA GCATCCCTAA GGATGTTATC ACTGAATGGC TGCAAGGCAT CTCCAGAGAG 
CCCTCCAGGC TGGGGTCTAA GTGGACTGTC ACACACCCAA GGTTCCGGCG GGACACAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCT AACGTGGTGG ATGAAAACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTCGAT GGTACCCTGC TGGCCGGCCA GATGGACCTT 
GTGAATGAAA TTCCCTTTAC CTACGAGCAG CTCAGCATCT TCAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC CGAGTCCCTG ATCAAGCAGC TGGGCCACTT CTTCAGATAC 
GTTAGCCCTG AGGACATCCG GCAGTGGAAT GTGACTTCAC CAGACACAGT GAATACTCTG 
CTTAAAGTCA GCAAAGGACA AAAGATGGAT GCTCAGGTGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCAAGCT GGATGAGGAC ATAGTAAAAG CCCTGGACAA CATCCCCTTA 
AGTTACCTAT GTGACTTCAG CCCCCAGGAT CTGCACGCTA TACCCTCCAG TGTTATGTGG 
CTGGTTGGGC TCCATGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTAT CCTCTATCAG 
AAGGCCTGCT CAGCCTTCCA GAACGTGAGC GGGCTGGAAT ACTTTGAGAA AATCAGGACA 
TTTCTGGGTG GGGCCTCCAG GGAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGTATG 
GACATAGCCA CTTTCAAGAA GCTGCAGGTG GATGCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTAGGGCC ACACATTGGG GACCTGAAGA CTGAGGAGGA TAAAAGCCCT 
GTCCGGGACT GGCTCTTCCG ACAGCAGCAG AAAGACCTGG ACAGTCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGATC CTAGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGTGGAGCCC CACTCCTTGG GCCAGGATTT GTGTTTGCAT GGATTCCAGC TCTGCTCTCA 
GCTTTAAGAC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msln ( XM_006246021.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246083.1 Rattus norvegicus mesothelin (Msln), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32915
Clone ID Related Accession (Same CDS sequence)	XM_006246022.2 , XM_006246021.3
Accession Version	XM_006246021.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	mesothelin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246021.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGC CAACAGCCCA ACCCCTGCTG GGGTCCTGTG GAAGCCCCAT CTGCAGCCGC 
AGCTTTCTAC TGCTTCTCCT TAGTCTTGGG TGGTTGCCAC TTCTGCAGAC CCAGACTACA 
AGGACAAGCC AGGAGGCCGC ACTTCTCCAT GCTGTGACCG GCACCGTTGA CTTTGCCAGT 
CTTCCCACAG GCCTCTTTCT TGGCCTCATG TGTGATGAGG TATCTGGCCT AAGCATGGGA 
CACGCCAAGG AGCTGGCTAT GGCTGTGAGA CAGAAGAATA TCGTGCTCCA AGTACATCAG 
CTGCGCTGTC TGGCCCGTCG CCTCCCTAAG CACCTCACCA ACGAGGAACT GGATGCTCTC 
CCACTGGACC TGCTGCTCTT CCTCAATCCA GCCATGTTTC CGGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTAAATGTAC TCCCACGGAG ATCTCTGGAG 
CGCCAGAGGC TGCTGACCGG GGCTCTGAAA TGCCAGGGTG TGTATGGATT TCAAGTGAGT 
GAGACGGATG CACGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGGA ATTCGTGGCC 
AAATCTTCGG AAGTCCTCCT CCCCTGGCTG GCAAGATGCG GAGGACCCCT GGACCAAGGC 
CAGGCAAAGG CTGTCAGGGA GGTTCTGAGG AGTGGAAGAG CCCCCTATGG TCCCCCATCG 
ACGTGGTCAG TCTCCACCCT GGATGCCCTG CAGGGTTTGC TGGTAGTGTT GGATGAGTCC 
ATTGTCCACA GCATCCCTAA GGATGTTATC ACTGAATGGC TGCAAGGCAT CTCCAGAGAG 
CCCTCCAGGC TGGGGTCTAA GTGGACTGTC ACACACCCAA GGTTCCGGCG GGACACAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCT AACGTGGTGG ATGAAAACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTCGAT GGTACCCTGC TGGCCGGCCA GATGGACCTT 
GTGAATGAAA TTCCCTTTAC CTACGAGCAG CTCAGCATCT TCAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC CGAGTCCCTG ATCAAGCAGC TGGGCCACTT CTTCAGATAC 
GTTAGCCCTG AGGACATCCG GCAGTGGAAT GTGACTTCAC CAGACACAGT GAATACTCTG 
CTTAAAGTCA GCAAAGGACA AAAGATGGAT GCTCAGGTGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCAAGCT GGATGAGGAC ATAGTAAAAG CCCTGGACAA CATCCCCTTA 
AGTTACCTAT GTGACTTCAG CCCCCAGGAT CTGCACGCTA TACCCTCCAG TGTTATGTGG 
CTGGTTGGGC TCCATGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTAT CCTCTATCAG 
AAGGCCTGCT CAGCCTTCCA GAACGTGAGC GGGCTGGAAT ACTTTGAGAA AATCAGGACA 
TTTCTGGGTG GGGCCTCCAG GGAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGTATG 
GACATAGCCA CTTTCAAGAA GCTGCAGGTG GATGCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTAGGGCC ACACATTGGG GACCTGAAGA CTGAGGAGGA TAAAAGCCCT 
GTCCGGGACT GGCTCTTCCG ACAGCAGCAG AAAGACCTGG ACAGTCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGATC CTAGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGTGGAGCCC CACTCCTTGG GCCAGGATTT GTGTTTGCAT GGATTCCAGC TCTGCTCTCA 
GCTTTAAGAC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246083.1
CDS	43..1920
Translation	MALPTAQPLLGSCGSPICSRSFLLLLLSLGWLPLLQTQTTRTSQEAALLHAVTGTVDFASLPTGLFLGLMCDEVSGLSMGHAKELAMAVRQKNIVLQVHQLRCLARRLPKHLTNEELDALPLDLLLFLNPAMFPGQQACAHFFSLISKANVNVLPRRSLERQRLLTGALKCQGVYGFQVSETDARALGGLACDLPGEFVAKSSEVLLPWLARCGGPLDQGQAKAVREVLRSGRAPYGPPSTWSVSTLDALQGLLVVLDESIVHSIPKDVITEWLQGISREPSRLGSKWTVTHPRFRRDTEQKACPPGKEPNVVDENLIFYQNWELEACVDGTLLAGQMDLVNEIPFTYEQLSIFKHKLDKTYPQGYPESLIKQLGHFFRYVSPEDIRQWNVTSPDTVNTLLKVSKGQKMDAQVIALVACYLRGGGKLDEDIVKALDNIPLSYLCDFSPQDLHAIPSSVMWLVGLHDLDKCSQRHLGILYQKACSAFQNVSGLEYFEKIRTFLGGASREDLRALSQHNVSMDIATFKKLQVDALVGLSVAEVQKLLGPHIGDLKTEEDKSPVRDWLFRQQQKDLDSLGLGLQGGIPNGYLILDFNVREAFSSGAPLLGPGFVFAWIPALLSALRLS

Target ORF information:

RefSeq Version	XM_006246021.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mesothelin (Msln), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246021.3

ORF Insert Sequence:

ATGGCCTTGC CAACAGCCCA ACCCCTGCTG GGGTCCTGTG GAAGCCCCAT CTGCAGCCGC 
AGCTTTCTAC TGCTTCTCCT TAGTCTTGGG TGGTTGCCAC TTCTGCAGAC CCAGACTACA 
AGGACAAGCC AGGAGGCCGC ACTTCTCCAT GCTGTGACCG GCACCGTTGA CTTTGCCAGT 
CTTCCCACAG GCCTCTTTCT TGGCCTCATG TGTGATGAGG TATCTGGCCT AAGCATGGGA 
CACGCCAAGG AGCTGGCTAT GGCTGTGAGA CAGAAGAATA TCGTGCTCCA AGTACATCAG 
CTGCGCTGTC TGGCCCGTCG CCTCCCTAAG CACCTCACCA ACGAGGAACT GGATGCTCTC 
CCACTGGACC TGCTGCTCTT CCTCAATCCA GCCATGTTTC CGGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTAAATGTAC TCCCACGGAG ATCTCTGGAG 
CGCCAGAGGC TGCTGACCGG GGCTCTGAAA TGCCAGGGTG TGTATGGATT TCAAGTGAGT 
GAGACGGATG CACGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGGA ATTCGTGGCC 
AAATCTTCGG AAGTCCTCCT CCCCTGGCTG GCAAGATGCG GAGGACCCCT GGACCAAGGC 
CAGGCAAAGG CTGTCAGGGA GGTTCTGAGG AGTGGAAGAG CCCCCTATGG TCCCCCATCG 
ACGTGGTCAG TCTCCACCCT GGATGCCCTG CAGGGTTTGC TGGTAGTGTT GGATGAGTCC 
ATTGTCCACA GCATCCCTAA GGATGTTATC ACTGAATGGC TGCAAGGCAT CTCCAGAGAG 
CCCTCCAGGC TGGGGTCTAA GTGGACTGTC ACACACCCAA GGTTCCGGCG GGACACAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCT AACGTGGTGG ATGAAAACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTCGAT GGTACCCTGC TGGCCGGCCA GATGGACCTT 
GTGAATGAAA TTCCCTTTAC CTACGAGCAG CTCAGCATCT TCAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC CGAGTCCCTG ATCAAGCAGC TGGGCCACTT CTTCAGATAC 
GTTAGCCCTG AGGACATCCG GCAGTGGAAT GTGACTTCAC CAGACACAGT GAATACTCTG 
CTTAAAGTCA GCAAAGGACA AAAGATGGAT GCTCAGGTGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCAAGCT GGATGAGGAC ATAGTAAAAG CCCTGGACAA CATCCCCTTA 
AGTTACCTAT GTGACTTCAG CCCCCAGGAT CTGCACGCTA TACCCTCCAG TGTTATGTGG 
CTGGTTGGGC TCCATGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTAT CCTCTATCAG 
AAGGCCTGCT CAGCCTTCCA GAACGTGAGC GGGCTGGAAT ACTTTGAGAA AATCAGGACA 
TTTCTGGGTG GGGCCTCCAG GGAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGTATG 
GACATAGCCA CTTTCAAGAA GCTGCAGGTG GATGCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTAGGGCC ACACATTGGG GACCTGAAGA CTGAGGAGGA TAAAAGCCCT 
GTCCGGGACT GGCTCTTCCG ACAGCAGCAG AAAGACCTGG ACAGTCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGATC CTAGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGTGGAGCCC CACTCCTTGG GCCAGGATTT GTGTTTGCAT GGATTCCAGC TCTGCTCTCA 
GCTTTAAGAC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msln ( NM_031658.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113846.1 Rattus norvegicus mesothelin (Msln), mRNA.

Price & Availability↑

CloneID	ORa08403
Clone ID Related Accession (Same CDS sequence)	NM_031658.1
Accession Version	NM_031658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	mesothelin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D87351.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D87351.1, BC070934.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906357, SAMN01906358 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTGC CAACAGCCCA ACCCCTGCTG GGGTCCTGTG GAAGCCCCAT CTGCAGCCGC 
AGCTTTCTAC TGCTTCTCCT TAGTCTTGGG TGGTTGCCAC TTCTGCAGAC CCAGACTACA 
AGGACAAGCC AGGAGGCCGC ACTTCTCCAT GCTGTGACCG GCACCGTTGA CTTTGCCAGT 
CTTCCCACAG GCCTCTTTCT TGGCCTCACG TGTGATGAGG TATCTGGCCT AAGCATGGGA 
CACGCCAAGG AGCTGGCTAT GGCTGTGAGA CAGAAGAATA TCGTGCTCCA AGTACATCAG 
CTGCGCTGTC TGGCCCGTCG CCTCCCTAAG CACCTCACCA ACGAGGAACT GGATGCTCTC 
CCACTGGACC TGCTGCTCTT CCTCAATCCA GCCATGTTTC CGGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTAAATGTAC TCCCACGGAG ATCTCTGGAG 
CGCCAGAGGC TGCTGACCGG GGCTCTGAAA TGCCAGGGTG TGTATGGATT TCAAGTGAGT 
GAGACGGATG CACGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGGA ATTCGTGGCC 
AAATCTTCGG AAGTCCTCCT CCCCTGGCTG GCAAGATGCG GAGGACCCCT GGACCAAGGC 
CAGGCAAAGG CTGTCAGGGA GGTTCTGAGG AGTGGAAGAG CCCCCTATGG TCCCCCATCG 
ACGTGGTCAG TCTCCACCCT GGATGCCCTG CAGGGTTTGC TGGTAGTGTT GGATGAGTCC 
ATTGTCCACA GCATCCCTAA GGATGTTATC ACTGAATGGC TGCAAGGCAT CTCCAGAGAG 
CCCTCCAGGC TGGGGTCTAA GTGGACTGTC ACACACCCAA GGTTCCGGCG GGACACAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCT AACGTGGTGG ATGAAAACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTCGAT GGTACCCTGC TGGCCGGCCA GATGGACCTT 
GTGAATGAAA TTCCCTTTAC CTACGAGCAG CTCAGCATCT TCAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC CGAGTCCCTG ATCAAGCAGC TGGGCCACTT CTTCAGATAC 
GTTAGCCCTG AGGACATCCG GCAGTGGAAT GTGACTTCAC CAGACACAGT GAATACTCTG 
CTTAAAGTCA GCAAAGGACA AAAGATGGAT GCTCAGGTGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCAAGCT GGACGAGGAC ATAGTAAAAG CCCTGGACAA CATCCCCTTA 
AGTTACCTAT GTGACTTCAG CCCCCAGGAT CTGCACGCTA TACCCTCCAG TGTTATGTGG 
CTGGTTGGGC TCCATGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTAT CCTCTATCAG 
AAGGCCTGCT CAGCCTTCCA GAACGTGAGC GGGCTGGAAT ACTTTGAGAA AATCAGGACA 
TTTCTGGGTG GGGCCTCCAG GGAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGTATG 
GACATAGCCA CTTTCAAGAA GCTGCAGGTG GATGCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTAGGGCC ACACATTGGG GACCTGAAGA CTGAGGAGGA TAAAAGCCCT 
GTCCGGGACT GGCTCTTCCG ACAGCAGCAG AAAGACCTGG ACAGTCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGATC CTAGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGTGGAGCCC CACTCCTTGG GCCAGGATTT GTGTTTGCAT GGATTCCAGC TCTGCTCTCA 
GCTTTAAGAC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113846.1
CDS	91..1968
Translation	MALPTAQPLLGSCGSPICSRSFLLLLLSLGWLPLLQTQTTRTSQEAALLHAVTGTVDFASLPTGLFLGLTCDEVSGLSMGHAKELAMAVRQKNIVLQVHQLRCLARRLPKHLTNEELDALPLDLLLFLNPAMFPGQQACAHFFSLISKANVNVLPRRSLERQRLLTGALKCQGVYGFQVSETDARALGGLACDLPGEFVAKSSEVLLPWLARCGGPLDQGQAKAVREVLRSGRAPYGPPSTWSVSTLDALQGLLVVLDESIVHSIPKDVITEWLQGISREPSRLGSKWTVTHPRFRRDTEQKACPPGKEPNVVDENLIFYQNWELEACVDGTLLAGQMDLVNEIPFTYEQLSIFKHKLDKTYPQGYPESLIKQLGHFFRYVSPEDIRQWNVTSPDTVNTLLKVSKGQKMDAQVIALVACYLRGGGKLDEDIVKALDNIPLSYLCDFSPQDLHAIPSSVMWLVGLHDLDKCSQRHLGILYQKACSAFQNVSGLEYFEKIRTFLGGASREDLRALSQHNVSMDIATFKKLQVDALVGLSVAEVQKLLGPHIGDLKTEEDKSPVRDWLFRQQQKDLDSLGLGLQGGIPNGYLILDFNVREAFSSGAPLLGPGFVFAWIPALLSALRLS

Target ORF information:

RefSeq Version	NM_031658.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mesothelin (Msln), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031658.1

ORF Insert Sequence:

ATGGCCTTGC CAACAGCCCA ACCCCTGCTG GGGTCCTGTG GAAGCCCCAT CTGCAGCCGC 
AGCTTTCTAC TGCTTCTCCT TAGTCTTGGG TGGTTGCCAC TTCTGCAGAC CCAGACTACA 
AGGACAAGCC AGGAGGCCGC ACTTCTCCAT GCTGTGACCG GCACCGTTGA CTTTGCCAGT 
CTTCCCACAG GCCTCTTTCT TGGCCTCACG TGTGATGAGG TATCTGGCCT AAGCATGGGA 
CACGCCAAGG AGCTGGCTAT GGCTGTGAGA CAGAAGAATA TCGTGCTCCA AGTACATCAG 
CTGCGCTGTC TGGCCCGTCG CCTCCCTAAG CACCTCACCA ACGAGGAACT GGATGCTCTC 
CCACTGGACC TGCTGCTCTT CCTCAATCCA GCCATGTTTC CGGGGCAACA GGCTTGTGCC 
CACTTCTTCT CCCTCATCTC TAAAGCCAAT GTAAATGTAC TCCCACGGAG ATCTCTGGAG 
CGCCAGAGGC TGCTGACCGG GGCTCTGAAA TGCCAGGGTG TGTATGGATT TCAAGTGAGT 
GAGACGGATG CACGGGCTCT CGGAGGCCTG GCCTGTGACC TGCCTGGGGA ATTCGTGGCC 
AAATCTTCGG AAGTCCTCCT CCCCTGGCTG GCAAGATGCG GAGGACCCCT GGACCAAGGC 
CAGGCAAAGG CTGTCAGGGA GGTTCTGAGG AGTGGAAGAG CCCCCTATGG TCCCCCATCG 
ACGTGGTCAG TCTCCACCCT GGATGCCCTG CAGGGTTTGC TGGTAGTGTT GGATGAGTCC 
ATTGTCCACA GCATCCCTAA GGATGTTATC ACTGAATGGC TGCAAGGCAT CTCCAGAGAG 
CCCTCCAGGC TGGGGTCTAA GTGGACTGTC ACACACCCAA GGTTCCGGCG GGACACAGAA 
CAGAAAGCCT GCCCTCCAGG GAAGGAGCCT AACGTGGTGG ATGAAAACCT CATCTTCTAC 
CAGAATTGGG AGCTGGAGGC TTGTGTCGAT GGTACCCTGC TGGCCGGCCA GATGGACCTT 
GTGAATGAAA TTCCCTTTAC CTACGAGCAG CTCAGCATCT TCAAGCACAA ACTGGACAAG 
ACCTACCCAC AAGGCTATCC CGAGTCCCTG ATCAAGCAGC TGGGCCACTT CTTCAGATAC 
GTTAGCCCTG AGGACATCCG GCAGTGGAAT GTGACTTCAC CAGACACAGT GAATACTCTG 
CTTAAAGTCA GCAAAGGACA AAAGATGGAT GCTCAGGTGA TTGCCTTGGT CGCCTGCTAT 
CTTCGGGGAG GAGGCAAGCT GGACGAGGAC ATAGTAAAAG CCCTGGACAA CATCCCCTTA 
AGTTACCTAT GTGACTTCAG CCCCCAGGAT CTGCACGCTA TACCCTCCAG TGTTATGTGG 
CTGGTTGGGC TCCATGACCT GGACAAGTGC AGCCAGAGGC ATCTGGGTAT CCTCTATCAG 
AAGGCCTGCT CAGCCTTCCA GAACGTGAGC GGGCTGGAAT ACTTTGAGAA AATCAGGACA 
TTTCTGGGTG GGGCCTCCAG GGAGGACCTG CGGGCCCTCA GCCAGCACAA TGTGAGTATG 
GACATAGCCA CTTTCAAGAA GCTGCAGGTG GATGCCCTGG TGGGGCTGAG TGTGGCTGAG 
GTACAGAAAC TTCTAGGGCC ACACATTGGG GACCTGAAGA CTGAGGAGGA TAAAAGCCCT 
GTCCGGGACT GGCTCTTCCG ACAGCAGCAG AAAGACCTGG ACAGTCTGGG TTTGGGACTT 
CAGGGTGGCA TCCCCAATGG CTACCTGATC CTAGACTTCA ATGTCCGAGA GGCCTTCTCC 
AGTGGAGCCC CACTCCTTGG GCCAGGATTT GTGTTTGCAT GGATTCCAGC TCTGCTCTCA 
GCTTTAAGAC TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas.
World journal of gastroenterology20(22)6884-96(2014 Jun)
Yin DD,You LH,Yuan QX,Liang XD,Wang N,Wang LT,Yuan L,Wang KM,De W

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Effectiveness of serum megakaryocyte potentiating factor in evaluating the effects of chrysotile and its heated products on respiratory organs.
Toxicology and applied pharmacology252(2)123-9(2011 Apr)
Takata A,Yamauchi H,Toya T,Miyamoto-Kohno S,Iwatatsu Y,Teranaka I,Aminaka M,Yamashita K,Kohyama N

An animal model of preclinical diagnosis of pancreatic ductal adenocarcinomas.
Biochemical and biophysical research communications390(3)636-41(2009 Dec)
Fukamachi K,Tanaka H,Hagiwara Y,Ohara H,Joh T,Iigo M,Alexander DB,Xu J,Long N,Takigahira M,Yanagihara K,Hino O,Saito I,Tsuda H

Circulating mesothelin protein and cellular antimesothelin immunity in patients with pancreatic cancer.
Clinical cancer research : an official journal of the American Association for Cancer Research15(21)6511-8(2009 Nov)
Johnston FM,Tan MC,Tan BR Jr,Porembka MR,Brunt EM,Linehan DC,Simon PO Jr,Plambeck-Suess S,Eberlein TJ,Hellstrom KE,Hellstrom I,Hawkins WG,Goedegebuure P

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Msln cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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