Folh1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Folh1
Entrez Gene ID	53320
Full Name	folate hydrolase 1
Synonyms	GCP2,mopsm
General protein information	Preferred Names folate hydrolase 1 Names glutamate carboxypeptidase 2 FGCP GCPII N-acetylated-alpha-linked acidic dipeptidase I NAALADase I folylpoly-gamma-glutamate carboxypeptidase glutamate carboxypeptidase II mGCP membrane glutamate carboxypeptidase prostate-specific membrane antigen homolog pteroylpoly-gamma-glutamate carboxypeptidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 D3

mRNA and Protein(s)

mRNA	Protein	Name
XM_011241861.2	XP_011240163.1	glutamate carboxypeptidase 2 isoform X2
XM_006508023.4	XP_006508086.1	glutamate carboxypeptidase 2 isoform X1
NM_001159706.1	NP_001153178.1	glutamate carboxypeptidase 2 isoform 2
NM_016770.3	NP_058050.3	glutamate carboxypeptidase 2 isoform 1
XM_006508023.3	XP_006508086.1	glutamate carboxypeptidase 2 isoform X1
XM_011241861.3	XP_011240163.1	glutamate carboxypeptidase 2 isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FOLH1	NP_001258707.1
Bos taurus (cattle)	FOLH1	NP_001095328.1
	FOLH1	XP_001096141.2
Mus musculus (house mouse)	Folh1	NP_058050.3
Neurospora crassa	NCU03108	XP_964967.2
Pan troglodytes (chimpanzee)	LOC451475	XP_001137174.1
Kluyveromyces lactis	KLLA0D16588g	XP_453792.1
Xenopus tropicalis (tropical clawed frog)	folh1	XP_002936599.2
Rattus norvegicus (Norway rat)	Folh1	NP_476533.1
Gallus gallus (chicken)	FOLH1	XP_417205.3
Magnaporthe oryzae (rice blast fungus)	MGG_15288	XP_003716179.1
Danio rerio (zebrafish)	naalad2	NP_956571.1

Gene Ontology
Bibliography

Related articles in PubMed

Elimination of the male reproductive tract in the female embryo is promoted by COUP-TFII in mice.
Zhao F, Franco HL, Rodriguez KF, Brown PR, Tsai MJ, Tsai SY, Yao HH
Science (New York, N.Y.)357(6352)717-720(2017 08)

PSMA redirects cell survival signaling from the MAPK to the PI3K-AKT pathways to promote the progression of prostate cancer.
Caromile LA, Dortche K, Rahman MM, Grant CL, Stoddard C, Ferrer FA, Shapiro LH
Science signaling10(470)(2017 Mar)

Glutamate carboxypeptidase II gene knockout attenuates oxidative stress and cortical apoptosis after traumatic brain injury.
Cao Y, Gao Y, Xu S, Bao J, Lin Y, Luo X, Wang Y, Luo Q, Jiang J, Neale JH, Zhong C
BMC neuroscience1715(2016 Apr)

Mice lacking glutamate carboxypeptidase II develop normally, but are less susceptible to traumatic brain injury.
Gao Y, Xu S, Cui Z, Zhang M, Lin Y, Cai L, Wang Z, Luo X, Zheng Y, Wang Y, Luo Q, Jiang J, Neale JH, Zhong C
Journal of neurochemistry134(2)340-53(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data support the hypothesis that the neuroprotective efficacy of glutamate carboxypeptidase II knockout in traumatic brain injury is mediated via a reduction in oxidative stress.
Title: Glutamate carboxypeptidase II gene knockout attenuates oxidative stress and cortical apoptosis after traumatic brain injury.

Knockout of the GCPII gene is not embryonic lethal and affords histopathological protection with improved long-term behavioral outcomes after traumatic brain injury
Title: Mice lacking glutamate carboxypeptidase II develop normally, but are less susceptible to traumatic brain injury.

Inhibition of glutamate carboxypeptidase II (GCPII) activity as a treatment for cognitive impairment in multiple sclerosis.
Title: Inhibition of glutamate carboxypeptidase II (GCPII) activity as a treatment for cognitive impairment in multiple sclerosis.

The combined effects of a haplo-insufficiency of glutamate carboxypeptidase II and dietary folic acid deficiency esult in reciprocal protection against cognitive and social deficits.
Title: Glutamate carboxypeptidase II and folate deficiencies result in reciprocal protection against cognitive and social deficits in mice: implications for neurodevelopmental disorders.

PSMA plays a VEGF-independent role in retinal angiogenesis
Title: Prostate specific membrane antigen (PSMA) regulates angiogenesis independently of VEGF during ocular neovascularization.

The following Folh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Folh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69470	XM_011241861.3 Latest version!	Mus musculus folate hydrolase 1 (Folh1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu07726	NM_001159706.1 Latest version!	Mus musculus folate hydrolase 1 (Folh1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00
OMu10647	NM_016770.3 Latest version!	Mus musculus folate hydrolase 1 (Folh1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$440.30 $629.00
OMu39768	XM_006508023.4 Latest version!	Mus musculus folate hydrolase 1 (Folh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu39768	XM_006508023.3	Mus musculus folate hydrolase 1 (Folh1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu69470	XM_011241861.2	Mus musculus folate hydrolase 1 (Folh1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Folh1 ( XM_011241861.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240163.1 Mus musculus folate hydrolase 1 (Folh1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu69470
Clone ID Related Accession (Same CDS sequence)	XM_011241861.2 , XM_011241861.3
Accession Version	XM_011241861.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glutamate carboxypeptidase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GTGATCTAGT GTATGTCAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGAAA TGAAGATCAG TTGTTCTGGG 
AAGATTGTGA TTGCCAGATA TGGGAAAGTG TTCAGAGGAA ATATGGTTAA AAATGCTCAA 
CTGGCAGGGG CAAAAGGAAT GATTCTGTAC TCAGACCCTG CTGACTACTT TGTTCCTGCG 
GTGAAGTCCT ATCCAGATGG CTGGAACCTC CCTGGAGGTG GTGTCCAACG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGACCCGCTC ACACCAGGTT ACCCAGCAAA TGAACATGCT 
TATAGGCATG AGTTGACAAA CGCTGTTGGC CTTCCAAGTA TTCCTGTCCA TCCTATTGGA 
TATGATGATG CACAGAAACT CTTAGAACAC ATGGGTGGTC CAGCACCCCC TGACAGTAGC 
TGGAAGGGAG GATTAAAAGT GCCTTACAAC GTGGGACCTG GCTTTGCTGG AAACTTTTCA 
ACACAAAAGG TCAAGATGCA TATTCACTCT TACACTAAAG TGACAAGAAT CTATAATGTC 
ATTGGCACCC TCAAAGGAGC TCTGGAACCA GACAGATATG TTATTCTTGG AGGTCACCGA 
GATGCTTGGG TATTTGGTGG CATTGACCCT CAGAGTGGAG CAGCTGTTGT TCATGAAATT 
GTGCGGAGCT TTGGAACCCT GAAGAAGAAA GGACGGAGGC CTAGAAGGAC AATTTTGTTT 
GCAAGCTGGG ATGCAGAAGA ATTTGGCCTT CTTGGTTCTA CTGAGTGGGC AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240163.1
CDS	296..1612
Translation	MWNALQDRDSAEVLGHRQRWLRVGTLVLALTGTFLIGFLFGWFIKPSNEATGNVSHSGMKKEFLHELKAENIKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEIFKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLEREMKISCSGKIVIARYGKVFRGNMVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEHAYRHELTNAVGLPSIPVHPIGYDDAQKLLEHMGGPAPPDSSWKGGLKVPYNVGPGFAGNFSTQKVKMHIHSYTKVTRIYNVIGTLKGALEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDAEEFGLLGSTEWAE

Target ORF information:

RefSeq Version	XM_011241861.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus folate hydrolase 1 (Folh1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241861.2

ORF Insert Sequence:

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GTGATCTAGT GTATGTCAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGAAA TGAAGATCAG TTGTTCTGGG 
AAGATTGTGA TTGCCAGATA TGGGAAAGTG TTCAGAGGAA ATATGGTTAA AAATGCTCAA 
CTGGCAGGGG CAAAAGGAAT GATTCTGTAC TCAGACCCTG CTGACTACTT TGTTCCTGCG 
GTGAAGTCCT ATCCAGATGG CTGGAACCTC CCTGGAGGTG GTGTCCAACG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGACCCGCTC ACACCAGGTT ACCCAGCAAA TGAACATGCT 
TATAGGCATG AGTTGACAAA CGCTGTTGGC CTTCCAAGTA TTCCTGTCCA TCCTATTGGA 
TATGATGATG CACAGAAACT CTTAGAACAC ATGGGTGGTC CAGCACCCCC TGACAGTAGC 
TGGAAGGGAG GATTAAAAGT GCCTTACAAC GTGGGACCTG GCTTTGCTGG AAACTTTTCA 
ACACAAAAGG TCAAGATGCA TATTCACTCT TACACTAAAG TGACAAGAAT CTATAATGTC 
ATTGGCACCC TCAAAGGAGC TCTGGAACCA GACAGATATG TTATTCTTGG AGGTCACCGA 
GATGCTTGGG TATTTGGTGG CATTGACCCT CAGAGTGGAG CAGCTGTTGT TCATGAAATT 
GTGCGGAGCT TTGGAACCCT GAAGAAGAAA GGACGGAGGC CTAGAAGGAC AATTTTGTTT 
GCAAGCTGGG ATGCAGAAGA ATTTGGCCTT CTTGGTTCTA CTGAGTGGGC AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Folh1 ( XM_011241861.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240163.1 Mus musculus folate hydrolase 1 (Folh1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu69470
Clone ID Related Accession (Same CDS sequence)	XM_011241861.2 , XM_011241861.3
Accession Version	XM_011241861.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glutamate carboxypeptidase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241861.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GTGATCTAGT GTATGTCAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGAAA TGAAGATCAG TTGTTCTGGG 
AAGATTGTGA TTGCCAGATA TGGGAAAGTG TTCAGAGGAA ATATGGTTAA AAATGCTCAA 
CTGGCAGGGG CAAAAGGAAT GATTCTGTAC TCAGACCCTG CTGACTACTT TGTTCCTGCG 
GTGAAGTCCT ATCCAGATGG CTGGAACCTC CCTGGAGGTG GTGTCCAACG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGACCCGCTC ACACCAGGTT ACCCAGCAAA TGAACATGCT 
TATAGGCATG AGTTGACAAA CGCTGTTGGC CTTCCAAGTA TTCCTGTCCA TCCTATTGGA 
TATGATGATG CACAGAAACT CTTAGAACAC ATGGGTGGTC CAGCACCCCC TGACAGTAGC 
TGGAAGGGAG GATTAAAAGT GCCTTACAAC GTGGGACCTG GCTTTGCTGG AAACTTTTCA 
ACACAAAAGG TCAAGATGCA TATTCACTCT TACACTAAAG TGACAAGAAT CTATAATGTC 
ATTGGCACCC TCAAAGGAGC TCTGGAACCA GACAGATATG TTATTCTTGG AGGTCACCGA 
GATGCTTGGG TATTTGGTGG CATTGACCCT CAGAGTGGAG CAGCTGTTGT TCATGAAATT 
GTGCGGAGCT TTGGAACCCT GAAGAAGAAA GGACGGAGGC CTAGAAGGAC AATTTTGTTT 
GCAAGCTGGG ATGCAGAAGA ATTTGGCCTT CTTGGTTCTA CTGAGTGGGC AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240163.1
CDS	295..1611
Translation	MWNALQDRDSAEVLGHRQRWLRVGTLVLALTGTFLIGFLFGWFIKPSNEATGNVSHSGMKKEFLHELKAENIKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEIFKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLEREMKISCSGKIVIARYGKVFRGNMVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEHAYRHELTNAVGLPSIPVHPIGYDDAQKLLEHMGGPAPPDSSWKGGLKVPYNVGPGFAGNFSTQKVKMHIHSYTKVTRIYNVIGTLKGALEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDAEEFGLLGSTEWAE

Target ORF information:

RefSeq Version	XM_011241861.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus folate hydrolase 1 (Folh1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241861.3

ORF Insert Sequence:

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GTGATCTAGT GTATGTCAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGAAA TGAAGATCAG TTGTTCTGGG 
AAGATTGTGA TTGCCAGATA TGGGAAAGTG TTCAGAGGAA ATATGGTTAA AAATGCTCAA 
CTGGCAGGGG CAAAAGGAAT GATTCTGTAC TCAGACCCTG CTGACTACTT TGTTCCTGCG 
GTGAAGTCCT ATCCAGATGG CTGGAACCTC CCTGGAGGTG GTGTCCAACG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGACCCGCTC ACACCAGGTT ACCCAGCAAA TGAACATGCT 
TATAGGCATG AGTTGACAAA CGCTGTTGGC CTTCCAAGTA TTCCTGTCCA TCCTATTGGA 
TATGATGATG CACAGAAACT CTTAGAACAC ATGGGTGGTC CAGCACCCCC TGACAGTAGC 
TGGAAGGGAG GATTAAAAGT GCCTTACAAC GTGGGACCTG GCTTTGCTGG AAACTTTTCA 
ACACAAAAGG TCAAGATGCA TATTCACTCT TACACTAAAG TGACAAGAAT CTATAATGTC 
ATTGGCACCC TCAAAGGAGC TCTGGAACCA GACAGATATG TTATTCTTGG AGGTCACCGA 
GATGCTTGGG TATTTGGTGG CATTGACCCT CAGAGTGGAG CAGCTGTTGT TCATGAAATT 
GTGCGGAGCT TTGGAACCCT GAAGAAGAAA GGACGGAGGC CTAGAAGGAC AATTTTGTTT 
GCAAGCTGGG ATGCAGAAGA ATTTGGCCTT CTTGGTTCTA CTGAGTGGGC AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Folh1 ( NM_001159706.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001153178.1 Mus musculus folate hydrolase 1 (Folh1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu07726
Clone ID Related Accession (Same CDS sequence)	NM_001159706.1
Accession Version	NM_001159706.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	glutamate carboxypeptidase 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB872570.1, BC119604.1, AK002920.1, BC119605.1 and BB179784.1. Transcript Variant: This variant (2) lacks an exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC119604.1, SRR1660825.43941.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GTGATCTAGT GTATGTCAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGAAA TGAAGATCAG TTGTTCTGGG 
AAGATTGTGA TTGCCAGATA TGGGAAAGTG TTCAGAGGAA ATATGGTTAA AAATGCTCAA 
CTGGCAGGGG CAAAAGGAAT GATTCTGTAC TCAGACCCTG CTGACTACTT TGTTCCTGCG 
GTGAAGTCCT ATCCAGATGG CTGGAACCTC CCTGGAGGTG GTGTCCAACG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGACCCGCTC ACACCAGGTT ACCCAGCAAA TGAACATGCT 
TATAGGCATG AGTTGACAAA CGCTGTTGGC CTTCCAAGTA TTCCTGTCCA TCCTATTGGA 
TATGATGATG CACAGAAACT CTTAGAAAAG GTCAAGATGC ATATTCACTC TTACACTAAA 
GTGACAAGAA TCTATAATGT CATTGGCACC CTCAAAGGAG CTCTGGAACC AGACAGATAT 
GTTATTCTTG GAGGTCACCG AGATGCTTGG GTATTTGGTG GCATTGACCC TCAGAGTGGA 
GCAGCTGTTG TTCATGAAAT TGTGCGGAGC TTTGGAACCC TGAAGAAGAA AGGACGGAGG 
CCTAGAAGGA CAATTTTGTT TGCAAGCTGG GATGCAGAAG AATTTGGCCT TCTTGGTTCT 
ACTGAGTGGG CAGAGGAACA TTCAAGACTC CTACAAGAGC GAGGTGTGGC TTATATTAAT 
GCTGATTCTT CCATAGAAGG AAATTACACT CTAAGAGTTG ATTGCACACC ACTGATGTAC 
AGCTTAGTGT ACAACCTAAC AAAAGAGCTG CAAAGCCCAG ATGAAGGTTT TGAAGGAAAA 
TCTCTTTATG ACAGCTGGAA AGAAAAGAGT CCTTCACCTG AGTTCATTGG AATGCCCAGA 
ATTAGCAAGC TGGGGTCTGG CAATGATTTT GAAGTGTTCT TCCAAAGACT TGGAATTGCT 
TCAGGCAGAG CCCGATATAC TAAAAATTGG AAAACTAACA AAGTCAGCAG CTATCCTCTC 
TATCACAGTG TCTATGAAAC ATATGAGCTG GTAGTAAAAT TTTATGACCC AACATTTAAA 
TACCACCTCA CTGTGGCCCA GGTTCGAGGA GCGATGGTAT TTGAACTTGC CAATTCTATA 
GTGCTTCCCT TTGACTGCCA AAGTTATGCT GTAGCTCTGA AGAAGTATGC TGACACTATC 
TACAATATTT CAATGAAACA TCCACAAGAA ATGAAGGCTT ACATGATATC ATTTGATTCA 
CTGTTTTCTG CAGTCAATAA TTTTACAGAT GTTGCATCTA AGTTCAATCA GAGACTGCAA 
GAGTTAGACA AAAGCAACCC CATATTACTG AGAATTATGA ATGACCAGCT GATGTATCTG 
GAACGTGCAT TCATTGATCC TTTAGGCTTA CCAGGAAGGC CTTTCTACAG GCATATCATC 
TATGCTCCAA GCAGCCACAA CAAGTATGCA GGAGAATCAT TCCCTGGGAT TTATGATGCC 
CTTTTTGATA TAAGTAGCAA AGTCAATGCT TCTAAGGCCT GGAACGAAGT GAAGAGACAG 
ATTTCTATTG CAACCTTTAC AGTGCAAGCT GCAGCAGAGA CTCTGAGGGA AGTAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153178.1
CDS	106..2265
Translation	MWNALQDRDSAEVLGHRQRWLRVGTLVLALTGTFLIGFLFGWFIKPSNEATGNVSHSGMKKEFLHELKAENIKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEIFKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLEREMKISCSGKIVIARYGKVFRGNMVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEHAYRHELTNAVGLPSIPVHPIGYDDAQKLLEKVKMHIHSYTKVTRIYNVIGTLKGALEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDAEEFGLLGSTEWAEEHSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPDEGFEGKSLYDSWKEKSPSPEFIGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWKTNKVSSYPLYHSVYETYELVVKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAVALKKYADTIYNISMKHPQEMKAYMISFDSLFSAVNNFTDVASKFNQRLQELDKSNPILLRIMNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDISSKVNASKAWNEVKRQISIATFTVQAAAETLREVA

Target ORF information:

RefSeq Version	NM_001159706.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus folate hydrolase 1 (Folh1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159706.1

ORF Insert Sequence:

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GTGATCTAGT GTATGTCAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGAAA TGAAGATCAG TTGTTCTGGG 
AAGATTGTGA TTGCCAGATA TGGGAAAGTG TTCAGAGGAA ATATGGTTAA AAATGCTCAA 
CTGGCAGGGG CAAAAGGAAT GATTCTGTAC TCAGACCCTG CTGACTACTT TGTTCCTGCG 
GTGAAGTCCT ATCCAGATGG CTGGAACCTC CCTGGAGGTG GTGTCCAACG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGACCCGCTC ACACCAGGTT ACCCAGCAAA TGAACATGCT 
TATAGGCATG AGTTGACAAA CGCTGTTGGC CTTCCAAGTA TTCCTGTCCA TCCTATTGGA 
TATGATGATG CACAGAAACT CTTAGAAAAG GTCAAGATGC ATATTCACTC TTACACTAAA 
GTGACAAGAA TCTATAATGT CATTGGCACC CTCAAAGGAG CTCTGGAACC AGACAGATAT 
GTTATTCTTG GAGGTCACCG AGATGCTTGG GTATTTGGTG GCATTGACCC TCAGAGTGGA 
GCAGCTGTTG TTCATGAAAT TGTGCGGAGC TTTGGAACCC TGAAGAAGAA AGGACGGAGG 
CCTAGAAGGA CAATTTTGTT TGCAAGCTGG GATGCAGAAG AATTTGGCCT TCTTGGTTCT 
ACTGAGTGGG CAGAGGAACA TTCAAGACTC CTACAAGAGC GAGGTGTGGC TTATATTAAT 
GCTGATTCTT CCATAGAAGG AAATTACACT CTAAGAGTTG ATTGCACACC ACTGATGTAC 
AGCTTAGTGT ACAACCTAAC AAAAGAGCTG CAAAGCCCAG ATGAAGGTTT TGAAGGAAAA 
TCTCTTTATG ACAGCTGGAA AGAAAAGAGT CCTTCACCTG AGTTCATTGG AATGCCCAGA 
ATTAGCAAGC TGGGGTCTGG CAATGATTTT GAAGTGTTCT TCCAAAGACT TGGAATTGCT 
TCAGGCAGAG CCCGATATAC TAAAAATTGG AAAACTAACA AAGTCAGCAG CTATCCTCTC 
TATCACAGTG TCTATGAAAC ATATGAGCTG GTAGTAAAAT TTTATGACCC AACATTTAAA 
TACCACCTCA CTGTGGCCCA GGTTCGAGGA GCGATGGTAT TTGAACTTGC CAATTCTATA 
GTGCTTCCCT TTGACTGCCA AAGTTATGCT GTAGCTCTGA AGAAGTATGC TGACACTATC 
TACAATATTT CAATGAAACA TCCACAAGAA ATGAAGGCTT ACATGATATC ATTTGATTCA 
CTGTTTTCTG CAGTCAATAA TTTTACAGAT GTTGCATCTA AGTTCAATCA GAGACTGCAA 
GAGTTAGACA AAAGCAACCC CATATTACTG AGAATTATGA ATGACCAGCT GATGTATCTG 
GAACGTGCAT TCATTGATCC TTTAGGCTTA CCAGGAAGGC CTTTCTACAG GCATATCATC 
TATGCTCCAA GCAGCCACAA CAAGTATGCA GGAGAATCAT TCCCTGGGAT TTATGATGCC 
CTTTTTGATA TAAGTAGCAA AGTCAATGCT TCTAAGGCCT GGAACGAAGT GAAGAGACAG 
ATTTCTATTG CAACCTTTAC AGTGCAAGCT GCAGCAGAGA CTCTGAGGGA AGTAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Folh1 ( NM_016770.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058050.3 Mus musculus folate hydrolase 1 (Folh1), transcript variant 1, mRNA.

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CloneID	OMu10647
Clone ID Related Accession (Same CDS sequence)	NM_016770.3
Accession Version	NM_016770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	glutamate carboxypeptidase 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB872570.1, BC119605.1, AK002920.1 and BB179784.1. On Apr 29, 2009 this sequence version replaced NM_016770.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK002920.1, SRR1660815.27886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GTGATCTAGT GTATGTCAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGAAA TGAAGATCAG TTGTTCTGGG 
AAGATTGTGA TTGCCAGATA TGGGAAAGTG TTCAGAGGAA ATATGGTTAA AAATGCTCAA 
CTGGCAGGGG CAAAAGGAAT GATTCTGTAC TCAGACCCTG CTGACTACTT TGTTCCTGCG 
GTGAAGTCCT ATCCAGATGG CTGGAACCTC CCTGGAGGTG GTGTCCAACG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGACCCGCTC ACACCAGGTT ACCCAGCAAA TGAACATGCT 
TATAGGCATG AGTTGACAAA CGCTGTTGGC CTTCCAAGTA TTCCTGTCCA TCCTATTGGA 
TATGATGATG CACAGAAACT CTTAGAACAC ATGGGTGGTC CAGCACCCCC TGACAGTAGC 
TGGAAGGGAG GATTAAAAGT GCCTTACAAC GTGGGACCTG GCTTTGCTGG AAACTTTTCA 
ACACAAAAGG TCAAGATGCA TATTCACTCT TACACTAAAG TGACAAGAAT CTATAATGTC 
ATTGGCACCC TCAAAGGAGC TCTGGAACCA GACAGATATG TTATTCTTGG AGGTCACCGA 
GATGCTTGGG TATTTGGTGG CATTGACCCT CAGAGTGGAG CAGCTGTTGT TCATGAAATT 
GTGCGGAGCT TTGGAACCCT GAAGAAGAAA GGACGGAGGC CTAGAAGGAC AATTTTGTTT 
GCAAGCTGGG ATGCAGAAGA ATTTGGCCTT CTTGGTTCTA CTGAGTGGGC AGAGGAACAT 
TCAAGACTCC TACAAGAGCG AGGTGTGGCT TATATTAATG CTGATTCTTC CATAGAAGGA 
AATTACACTC TAAGAGTTGA TTGCACACCA CTGATGTACA GCTTAGTGTA CAACCTAACA 
AAAGAGCTGC AAAGCCCAGA TGAAGGTTTT GAAGGAAAAT CTCTTTATGA CAGCTGGAAA 
GAAAAGAGTC CTTCACCTGA GTTCATTGGA ATGCCCAGAA TTAGCAAGCT GGGGTCTGGC 
AATGATTTTG AAGTGTTCTT CCAAAGACTT GGAATTGCTT CAGGCAGAGC CCGATATACT 
AAAAATTGGA AAACTAACAA AGTCAGCAGC TATCCTCTCT ATCACAGTGT CTATGAAACA 
TATGAGCTGG TAGTAAAATT TTATGACCCA ACATTTAAAT ACCACCTCAC TGTGGCCCAG 
GTTCGAGGAG CGATGGTATT TGAACTTGCC AATTCTATAG TGCTTCCCTT TGACTGCCAA 
AGTTATGCTG TAGCTCTGAA GAAGTATGCT GACACTATCT ACAATATTTC AATGAAACAT 
CCACAAGAAA TGAAGGCTTA CATGATATCA TTTGATTCAC TGTTTTCTGC AGTCAATAAT 
TTTACAGATG TTGCATCTAA GTTCAATCAG AGACTGCAAG AGTTAGACAA AAGCAACCCC 
ATATTACTGA GAATTATGAA TGACCAGCTG ATGTATCTGG AACGTGCATT CATTGATCCT 
TTAGGCTTAC CAGGAAGGCC TTTCTACAGG CATATCATCT ATGCTCCAAG CAGCCACAAC 
AAGTATGCAG GAGAATCATT CCCTGGGATT TATGATGCCC TTTTTGATAT AAGTAGCAAA 
GTCAATGCTT CTAAGGCCTG GAACGAAGTG AAGAGACAGA TTTCTATTGC AACCTTTACA 
GTGCAAGCTG CAGCAGAGAC TCTGAGGGAA GTAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058050.3
CDS	106..2364
Translation	MWNALQDRDSAEVLGHRQRWLRVGTLVLALTGTFLIGFLFGWFIKPSNEATGNVSHSGMKKEFLHELKAENIKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEIFKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLEREMKISCSGKIVIARYGKVFRGNMVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEHAYRHELTNAVGLPSIPVHPIGYDDAQKLLEHMGGPAPPDSSWKGGLKVPYNVGPGFAGNFSTQKVKMHIHSYTKVTRIYNVIGTLKGALEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDAEEFGLLGSTEWAEEHSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPDEGFEGKSLYDSWKEKSPSPEFIGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWKTNKVSSYPLYHSVYETYELVVKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAVALKKYADTIYNISMKHPQEMKAYMISFDSLFSAVNNFTDVASKFNQRLQELDKSNPILLRIMNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDISSKVNASKAWNEVKRQISIATFTVQAAAETLREVA

Target ORF information:

RefSeq Version	NM_016770.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus folate hydrolase 1 (Folh1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016770.3

ORF Insert Sequence:

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GTGATCTAGT GTATGTCAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGAAA TGAAGATCAG TTGTTCTGGG 
AAGATTGTGA TTGCCAGATA TGGGAAAGTG TTCAGAGGAA ATATGGTTAA AAATGCTCAA 
CTGGCAGGGG CAAAAGGAAT GATTCTGTAC TCAGACCCTG CTGACTACTT TGTTCCTGCG 
GTGAAGTCCT ATCCAGATGG CTGGAACCTC CCTGGAGGTG GTGTCCAACG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGACCCGCTC ACACCAGGTT ACCCAGCAAA TGAACATGCT 
TATAGGCATG AGTTGACAAA CGCTGTTGGC CTTCCAAGTA TTCCTGTCCA TCCTATTGGA 
TATGATGATG CACAGAAACT CTTAGAACAC ATGGGTGGTC CAGCACCCCC TGACAGTAGC 
TGGAAGGGAG GATTAAAAGT GCCTTACAAC GTGGGACCTG GCTTTGCTGG AAACTTTTCA 
ACACAAAAGG TCAAGATGCA TATTCACTCT TACACTAAAG TGACAAGAAT CTATAATGTC 
ATTGGCACCC TCAAAGGAGC TCTGGAACCA GACAGATATG TTATTCTTGG AGGTCACCGA 
GATGCTTGGG TATTTGGTGG CATTGACCCT CAGAGTGGAG CAGCTGTTGT TCATGAAATT 
GTGCGGAGCT TTGGAACCCT GAAGAAGAAA GGACGGAGGC CTAGAAGGAC AATTTTGTTT 
GCAAGCTGGG ATGCAGAAGA ATTTGGCCTT CTTGGTTCTA CTGAGTGGGC AGAGGAACAT 
TCAAGACTCC TACAAGAGCG AGGTGTGGCT TATATTAATG CTGATTCTTC CATAGAAGGA 
AATTACACTC TAAGAGTTGA TTGCACACCA CTGATGTACA GCTTAGTGTA CAACCTAACA 
AAAGAGCTGC AAAGCCCAGA TGAAGGTTTT GAAGGAAAAT CTCTTTATGA CAGCTGGAAA 
GAAAAGAGTC CTTCACCTGA GTTCATTGGA ATGCCCAGAA TTAGCAAGCT GGGGTCTGGC 
AATGATTTTG AAGTGTTCTT CCAAAGACTT GGAATTGCTT CAGGCAGAGC CCGATATACT 
AAAAATTGGA AAACTAACAA AGTCAGCAGC TATCCTCTCT ATCACAGTGT CTATGAAACA 
TATGAGCTGG TAGTAAAATT TTATGACCCA ACATTTAAAT ACCACCTCAC TGTGGCCCAG 
GTTCGAGGAG CGATGGTATT TGAACTTGCC AATTCTATAG TGCTTCCCTT TGACTGCCAA 
AGTTATGCTG TAGCTCTGAA GAAGTATGCT GACACTATCT ACAATATTTC AATGAAACAT 
CCACAAGAAA TGAAGGCTTA CATGATATCA TTTGATTCAC TGTTTTCTGC AGTCAATAAT 
TTTACAGATG TTGCATCTAA GTTCAATCAG AGACTGCAAG AGTTAGACAA AAGCAACCCC 
ATATTACTGA GAATTATGAA TGACCAGCTG ATGTATCTGG AACGTGCATT CATTGATCCT 
TTAGGCTTAC CAGGAAGGCC TTTCTACAGG CATATCATCT ATGCTCCAAG CAGCCACAAC 
AAGTATGCAG GAGAATCATT CCCTGGGATT TATGATGCCC TTTTTGATAT AAGTAGCAAA 
GTCAATGCTT CTAAGGCCTG GAACGAAGTG AAGAGACAGA TTTCTATTGC AACCTTTACA 
GTGCAAGCTG CAGCAGAGAC TCTGAGGGAA GTAGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Folh1 ( XM_006508023.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508086.1 Mus musculus folate hydrolase 1 (Folh1), transcript variant X1, mRNA.

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CloneID	OMu39768
Clone ID Related Accession (Same CDS sequence)	XM_006508023.4 , XM_006508023.3
Accession Version	XM_006508023.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glutamate carboxypeptidase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG TTAAAAATGC TCAACTGGCA 
GGGGCAAAAG GAATGATTCT GTACTCAGAC CCTGCTGACT ACTTTGTTCC TGCGGTGAAG 
TCCTATCCAG ATGGCTGGAA CCTCCCTGGA GGTGGTGTCC AACGTGGAAA TGTCTTAAAT 
CTTAATGGTG CAGGTGACCC GCTCACACCA GGTTACCCAG CAAATGAACA TGCTTATAGG 
CATGAGTTGA CAAACGCTGT TGGCCTTCCA AGTATTCCTG TCCATCCTAT TGGATATGAT 
GATGCACAGA AACTCTTAGA ACACATGGGT GGTCCAGCAC CCCCTGACAG TAGCTGGAAG 
GGAGGATTAA AAGTGCCTTA CAACGTGGGA CCTGGCTTTG CTGGAAACTT TTCAACACAA 
AAGGTCAAGA TGCATATTCA CTCTTACACT AAAGTGACAA GAATCTATAA TGTCATTGGC 
ACCCTCAAAG GAGCTCTGGA ACCAGACAGA TATGTTATTC TTGGAGGTCA CCGAGATGCT 
TGGGTATTTG GTGGCATTGA CCCTCAGAGT GGAGCAGCTG TTGTTCATGA AATTGTGCGG 
AGCTTTGGAA CCCTGAAGAA GAAAGGACGG AGGCCTAGAA GGACAATTTT GTTTGCAAGC 
TGGGATGCAG AAGAATTTGG CCTTCTTGGT TCTACTGAGT GGGCAGAGGA ACATTCAAGA 
CTCCTACAAG AGCGAGGTGT GGCTTATATT AATGCTGATT CTTCCATAGA AGGAAATTAC 
ACTCTAAGAG TTGATTGCAC ACCACTGATG TACAGCTTAG TGTACAACCT AACAAAAGAG 
CTGCAAAGCC CAGATGAAGG TTTTGAAGGA AAATCTCTTT ATGACAGCTG GAAAGAAAAG 
AGTCCTTCAC CTGAGTTCAT TGGAATGCCC AGAATTAGCA AGCTGGGGTC TGGCAATGAT 
TTTGAAGTGT TCTTCCAAAG ACTTGGAATT GCTTCAGGCA GAGCCCGATA TACTAAAAAT 
TGGAAAACTA ACAAAGTCAG CAGCTATCCT CTCTATCACA GTGTCTATGA AACATATGAG 
CTGGTAGTAA AATTTTATGA CCCAACATTT AAATACCACC TCACTGTGGC CCAGGTTCGA 
GGAGCGATGG TATTTGAACT TGCCAATTCT ATAGTGCTTC CCTTTGACTG CCAAAGTTAT 
GCTGTAGCTC TGAAGAAGTA TGCTGACACT ATCTACAATA TTTCAATGAA ACATCCACAA 
GAAATGAAGG CTTACATGAT ATCATTTGAT TCACTGTTTT CTGCAGTCAA TAATTTTACA 
GATGTTGCAT CTAAGTTCAA TCAGAGACTG CAAGAGTTAG ACAAAAGCAA CCCCATATTA 
CTGAGAATTA TGAATGACCA GCTGATGTAT CTGGAACGTG CATTCATTGA TCCTTTAGGC 
TTACCAGGAA GGCCTTTCTA CAGGCATATC ATCTATGCTC CAAGCAGCCA CAACAAGTAT 
GCAGGAGAAT CATTCCCTGG GATTTATGAT GCCCTTTTTG ATATAAGTAG CAAAGTCAAT 
GCTTCTAAGG CCTGGAACGA AGTGAAGAGA CAGATTTCTA TTGCAACCTT TACAGTGCAA 
GCTGCAGCAG AGACTCTGAG GGAAGTAGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508086.1
CDS	291..2423
Translation	MWNALQDRDSAEVLGHRQRWLRVGTLVLALTGTFLIGFLFGWFIKPSNEATGNVSHSGMKKEFLHELKAENIKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEIFKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEHAYRHELTNAVGLPSIPVHPIGYDDAQKLLEHMGGPAPPDSSWKGGLKVPYNVGPGFAGNFSTQKVKMHIHSYTKVTRIYNVIGTLKGALEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDAEEFGLLGSTEWAEEHSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPDEGFEGKSLYDSWKEKSPSPEFIGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWKTNKVSSYPLYHSVYETYELVVKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAVALKKYADTIYNISMKHPQEMKAYMISFDSLFSAVNNFTDVASKFNQRLQELDKSNPILLRIMNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDISSKVNASKAWNEVKRQISIATFTVQAAAETLREVA

Target ORF information:

RefSeq Version	XM_006508023.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus folate hydrolase 1 (Folh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508023.3

ORF Insert Sequence:

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG TTAAAAATGC TCAACTGGCA 
GGGGCAAAAG GAATGATTCT GTACTCAGAC CCTGCTGACT ACTTTGTTCC TGCGGTGAAG 
TCCTATCCAG ATGGCTGGAA CCTCCCTGGA GGTGGTGTCC AACGTGGAAA TGTCTTAAAT 
CTTAATGGTG CAGGTGACCC GCTCACACCA GGTTACCCAG CAAATGAACA TGCTTATAGG 
CATGAGTTGA CAAACGCTGT TGGCCTTCCA AGTATTCCTG TCCATCCTAT TGGATATGAT 
GATGCACAGA AACTCTTAGA ACACATGGGT GGTCCAGCAC CCCCTGACAG TAGCTGGAAG 
GGAGGATTAA AAGTGCCTTA CAACGTGGGA CCTGGCTTTG CTGGAAACTT TTCAACACAA 
AAGGTCAAGA TGCATATTCA CTCTTACACT AAAGTGACAA GAATCTATAA TGTCATTGGC 
ACCCTCAAAG GAGCTCTGGA ACCAGACAGA TATGTTATTC TTGGAGGTCA CCGAGATGCT 
TGGGTATTTG GTGGCATTGA CCCTCAGAGT GGAGCAGCTG TTGTTCATGA AATTGTGCGG 
AGCTTTGGAA CCCTGAAGAA GAAAGGACGG AGGCCTAGAA GGACAATTTT GTTTGCAAGC 
TGGGATGCAG AAGAATTTGG CCTTCTTGGT TCTACTGAGT GGGCAGAGGA ACATTCAAGA 
CTCCTACAAG AGCGAGGTGT GGCTTATATT AATGCTGATT CTTCCATAGA AGGAAATTAC 
ACTCTAAGAG TTGATTGCAC ACCACTGATG TACAGCTTAG TGTACAACCT AACAAAAGAG 
CTGCAAAGCC CAGATGAAGG TTTTGAAGGA AAATCTCTTT ATGACAGCTG GAAAGAAAAG 
AGTCCTTCAC CTGAGTTCAT TGGAATGCCC AGAATTAGCA AGCTGGGGTC TGGCAATGAT 
TTTGAAGTGT TCTTCCAAAG ACTTGGAATT GCTTCAGGCA GAGCCCGATA TACTAAAAAT 
TGGAAAACTA ACAAAGTCAG CAGCTATCCT CTCTATCACA GTGTCTATGA AACATATGAG 
CTGGTAGTAA AATTTTATGA CCCAACATTT AAATACCACC TCACTGTGGC CCAGGTTCGA 
GGAGCGATGG TATTTGAACT TGCCAATTCT ATAGTGCTTC CCTTTGACTG CCAAAGTTAT 
GCTGTAGCTC TGAAGAAGTA TGCTGACACT ATCTACAATA TTTCAATGAA ACATCCACAA 
GAAATGAAGG CTTACATGAT ATCATTTGAT TCACTGTTTT CTGCAGTCAA TAATTTTACA 
GATGTTGCAT CTAAGTTCAA TCAGAGACTG CAAGAGTTAG ACAAAAGCAA CCCCATATTA 
CTGAGAATTA TGAATGACCA GCTGATGTAT CTGGAACGTG CATTCATTGA TCCTTTAGGC 
TTACCAGGAA GGCCTTTCTA CAGGCATATC ATCTATGCTC CAAGCAGCCA CAACAAGTAT 
GCAGGAGAAT CATTCCCTGG GATTTATGAT GCCCTTTTTG ATATAAGTAG CAAAGTCAAT 
GCTTCTAAGG CCTGGAACGA AGTGAAGAGA CAGATTTCTA TTGCAACCTT TACAGTGCAA 
GCTGCAGCAG AGACTCTGAG GGAAGTAGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Folh1 ( XM_006508023.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508086.1 Mus musculus folate hydrolase 1 (Folh1), transcript variant X1, mRNA.

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CloneID	OMu39768
Clone ID Related Accession (Same CDS sequence)	XM_006508023.4 , XM_006508023.3
Accession Version	XM_006508023.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	glutamate carboxypeptidase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006508023.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG TTAAAAATGC TCAACTGGCA 
GGGGCAAAAG GAATGATTCT GTACTCAGAC CCTGCTGACT ACTTTGTTCC TGCGGTGAAG 
TCCTATCCAG ATGGCTGGAA CCTCCCTGGA GGTGGTGTCC AACGTGGAAA TGTCTTAAAT 
CTTAATGGTG CAGGTGACCC GCTCACACCA GGTTACCCAG CAAATGAACA TGCTTATAGG 
CATGAGTTGA CAAACGCTGT TGGCCTTCCA AGTATTCCTG TCCATCCTAT TGGATATGAT 
GATGCACAGA AACTCTTAGA ACACATGGGT GGTCCAGCAC CCCCTGACAG TAGCTGGAAG 
GGAGGATTAA AAGTGCCTTA CAACGTGGGA CCTGGCTTTG CTGGAAACTT TTCAACACAA 
AAGGTCAAGA TGCATATTCA CTCTTACACT AAAGTGACAA GAATCTATAA TGTCATTGGC 
ACCCTCAAAG GAGCTCTGGA ACCAGACAGA TATGTTATTC TTGGAGGTCA CCGAGATGCT 
TGGGTATTTG GTGGCATTGA CCCTCAGAGT GGAGCAGCTG TTGTTCATGA AATTGTGCGG 
AGCTTTGGAA CCCTGAAGAA GAAAGGACGG AGGCCTAGAA GGACAATTTT GTTTGCAAGC 
TGGGATGCAG AAGAATTTGG CCTTCTTGGT TCTACTGAGT GGGCAGAGGA ACATTCAAGA 
CTCCTACAAG AGCGAGGTGT GGCTTATATT AATGCTGATT CTTCCATAGA AGGAAATTAC 
ACTCTAAGAG TTGATTGCAC ACCACTGATG TACAGCTTAG TGTACAACCT AACAAAAGAG 
CTGCAAAGCC CAGATGAAGG TTTTGAAGGA AAATCTCTTT ATGACAGCTG GAAAGAAAAG 
AGTCCTTCAC CTGAGTTCAT TGGAATGCCC AGAATTAGCA AGCTGGGGTC TGGCAATGAT 
TTTGAAGTGT TCTTCCAAAG ACTTGGAATT GCTTCAGGCA GAGCCCGATA TACTAAAAAT 
TGGAAAACTA ACAAAGTCAG CAGCTATCCT CTCTATCACA GTGTCTATGA AACATATGAG 
CTGGTAGTAA AATTTTATGA CCCAACATTT AAATACCACC TCACTGTGGC CCAGGTTCGA 
GGAGCGATGG TATTTGAACT TGCCAATTCT ATAGTGCTTC CCTTTGACTG CCAAAGTTAT 
GCTGTAGCTC TGAAGAAGTA TGCTGACACT ATCTACAATA TTTCAATGAA ACATCCACAA 
GAAATGAAGG CTTACATGAT ATCATTTGAT TCACTGTTTT CTGCAGTCAA TAATTTTACA 
GATGTTGCAT CTAAGTTCAA TCAGAGACTG CAAGAGTTAG ACAAAAGCAA CCCCATATTA 
CTGAGAATTA TGAATGACCA GCTGATGTAT CTGGAACGTG CATTCATTGA TCCTTTAGGC 
TTACCAGGAA GGCCTTTCTA CAGGCATATC ATCTATGCTC CAAGCAGCCA CAACAAGTAT 
GCAGGAGAAT CATTCCCTGG GATTTATGAT GCCCTTTTTG ATATAAGTAG CAAAGTCAAT 
GCTTCTAAGG CCTGGAACGA AGTGAAGAGA CAGATTTCTA TTGCAACCTT TACAGTGCAA 
GCTGCAGCAG AGACTCTGAG GGAAGTAGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508086.1
CDS	290..2422
Translation	MWNALQDRDSAEVLGHRQRWLRVGTLVLALTGTFLIGFLFGWFIKPSNEATGNVSHSGMKKEFLHELKAENIKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEIFKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEHAYRHELTNAVGLPSIPVHPIGYDDAQKLLEHMGGPAPPDSSWKGGLKVPYNVGPGFAGNFSTQKVKMHIHSYTKVTRIYNVIGTLKGALEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDAEEFGLLGSTEWAEEHSRLLQERGVAYINADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPDEGFEGKSLYDSWKEKSPSPEFIGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWKTNKVSSYPLYHSVYETYELVVKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAVALKKYADTIYNISMKHPQEMKAYMISFDSLFSAVNNFTDVASKFNQRLQELDKSNPILLRIMNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDISSKVNASKAWNEVKRQISIATFTVQAAAETLREVA

Target ORF information:

RefSeq Version	XM_006508023.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus folate hydrolase 1 (Folh1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508023.4

ORF Insert Sequence:

ATGTGGAACG CACTGCAGGA CAGAGACTCC GCGGAGGTCC TGGGACACCG CCAGCGCTGG 
CTCCGTGTTG GGACACTGGT GCTGGCTTTA ACCGGAACCT TCCTCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCTTC CAATGAAGCT ACTGGTAATG TTTCCCATTC TGGCATGAAG 
AAGGAGTTTT TGCATGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ACTTGGCAGG AACACAAAAT AATTTTGAGC TTGCAAAGCA AATTCATGAC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCCC ATTACGATGT CTTGCTGTCC 
TATCCAAATA AAACTCATCC TAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTATCTGA ACAGCCACCC CCAGGATATG AGAATATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG TTAAAAATGC TCAACTGGCA 
GGGGCAAAAG GAATGATTCT GTACTCAGAC CCTGCTGACT ACTTTGTTCC TGCGGTGAAG 
TCCTATCCAG ATGGCTGGAA CCTCCCTGGA GGTGGTGTCC AACGTGGAAA TGTCTTAAAT 
CTTAATGGTG CAGGTGACCC GCTCACACCA GGTTACCCAG CAAATGAACA TGCTTATAGG 
CATGAGTTGA CAAACGCTGT TGGCCTTCCA AGTATTCCTG TCCATCCTAT TGGATATGAT 
GATGCACAGA AACTCTTAGA ACACATGGGT GGTCCAGCAC CCCCTGACAG TAGCTGGAAG 
GGAGGATTAA AAGTGCCTTA CAACGTGGGA CCTGGCTTTG CTGGAAACTT TTCAACACAA 
AAGGTCAAGA TGCATATTCA CTCTTACACT AAAGTGACAA GAATCTATAA TGTCATTGGC 
ACCCTCAAAG GAGCTCTGGA ACCAGACAGA TATGTTATTC TTGGAGGTCA CCGAGATGCT 
TGGGTATTTG GTGGCATTGA CCCTCAGAGT GGAGCAGCTG TTGTTCATGA AATTGTGCGG 
AGCTTTGGAA CCCTGAAGAA GAAAGGACGG AGGCCTAGAA GGACAATTTT GTTTGCAAGC 
TGGGATGCAG AAGAATTTGG CCTTCTTGGT TCTACTGAGT GGGCAGAGGA ACATTCAAGA 
CTCCTACAAG AGCGAGGTGT GGCTTATATT AATGCTGATT CTTCCATAGA AGGAAATTAC 
ACTCTAAGAG TTGATTGCAC ACCACTGATG TACAGCTTAG TGTACAACCT AACAAAAGAG 
CTGCAAAGCC CAGATGAAGG TTTTGAAGGA AAATCTCTTT ATGACAGCTG GAAAGAAAAG 
AGTCCTTCAC CTGAGTTCAT TGGAATGCCC AGAATTAGCA AGCTGGGGTC TGGCAATGAT 
TTTGAAGTGT TCTTCCAAAG ACTTGGAATT GCTTCAGGCA GAGCCCGATA TACTAAAAAT 
TGGAAAACTA ACAAAGTCAG CAGCTATCCT CTCTATCACA GTGTCTATGA AACATATGAG 
CTGGTAGTAA AATTTTATGA CCCAACATTT AAATACCACC TCACTGTGGC CCAGGTTCGA 
GGAGCGATGG TATTTGAACT TGCCAATTCT ATAGTGCTTC CCTTTGACTG CCAAAGTTAT 
GCTGTAGCTC TGAAGAAGTA TGCTGACACT ATCTACAATA TTTCAATGAA ACATCCACAA 
GAAATGAAGG CTTACATGAT ATCATTTGAT TCACTGTTTT CTGCAGTCAA TAATTTTACA 
GATGTTGCAT CTAAGTTCAA TCAGAGACTG CAAGAGTTAG ACAAAAGCAA CCCCATATTA 
CTGAGAATTA TGAATGACCA GCTGATGTAT CTGGAACGTG CATTCATTGA TCCTTTAGGC 
TTACCAGGAA GGCCTTTCTA CAGGCATATC ATCTATGCTC CAAGCAGCCA CAACAAGTAT 
GCAGGAGAAT CATTCCCTGG GATTTATGAT GCCCTTTTTG ATATAAGTAG CAAAGTCAAT 
GCTTCTAAGG CCTGGAACGA AGTGAAGAGA CAGATTTCTA TTGCAACCTT TACAGTGCAA 
GCTGCAGCAG AGACTCTGAG GGAAGTAGCT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Elimination of the male reproductive tract in the female embryo is promoted by COUP-TFII in mice.
Science (New York, N.Y.)357(6352)717-720(2017 08)
Zhao F,Franco HL,Rodriguez KF,Brown PR,Tsai MJ,Tsai SY,Yao HH

PSMA redirects cell survival signaling from the MAPK to the PI3K-AKT pathways to promote the progression of prostate cancer.
Science signaling10(470)(2017 Mar)
Caromile LA,Dortche K,Rahman MM,Grant CL,Stoddard C,Ferrer FA,Shapiro LH

Glutamate carboxypeptidase II gene knockout attenuates oxidative stress and cortical apoptosis after traumatic brain injury.
BMC neuroscience1715(2016 Apr)
Cao Y,Gao Y,Xu S,Bao J,Lin Y,Luo X,Wang Y,Luo Q,Jiang J,Neale JH,Zhong C

Mice lacking glutamate carboxypeptidase II develop normally, but are less susceptible to traumatic brain injury.
Journal of neurochemistry134(2)340-53(2015 Jul)
Gao Y,Xu S,Cui Z,Zhang M,Lin Y,Cai L,Wang Z,Luo X,Zheng Y,Wang Y,Luo Q,Jiang J,Neale JH,Zhong C

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Folh1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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