Folh1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Folh1
Entrez Gene ID	85309
Full Name	folate hydrolase 1
Synonyms	Naalad
General protein information	Preferred Names folate hydrolase 1 Names glutamate carboxypeptidase 2 FGCP GCPII N-acetylated alpha-linked acidic dipeptidase N-acetylated-alpha-linked acidic dipeptidase I NAALADase I folylpoly-gamma-glutamate carboxypeptidase glutamate carboxypeptidase II mGCP membrane glutamate carboxypeptidase prostate-specific membrane antigen homolog pteroylpoly-gamma-glutamate carboxypeptidase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q32
Summary	catalyzes the release of glutamate from acidic peptides including the neuropeptide N-acetyl-alpha-L-aspartyl-L-glutamate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_057185.2	NP_476533.1	glutamate carboxypeptidase 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	FOLH1	NP_001258707.1
Bos taurus (cattle)	FOLH1	NP_001095328.1
	FOLH1	XP_001096141.2
Mus musculus (house mouse)	Folh1	NP_058050.3
Neurospora crassa	NCU03108	XP_964967.2
Pan troglodytes (chimpanzee)	LOC451475	XP_001137174.1
Kluyveromyces lactis	KLLA0D16588g	XP_453792.1
Xenopus tropicalis (tropical clawed frog)	folh1	XP_002936599.2
Rattus norvegicus (Norway rat)	Folh1	NP_476533.1
Gallus gallus (chicken)	FOLH1	XP_417205.3
Magnaporthe oryzae (rice blast fungus)	MGG_15288	XP_003716179.1
Danio rerio (zebrafish)	naalad2	NP_956571.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural and biochemical characterization of the folyl-poly-?-l-glutamate hydrolyzing activity of human glutamate carboxypeptidase II.
Navr?til M, Pt??ek J, ??cha P, Starkov? J, Lubkowski J, Ba?inka C, Konvalinka J
The FEBS journal281(14)3228-42(2014 Jul)

Effects of N-acetylaspartylglutamate (NAAG) peptidase inhibition on release of glutamate and dopamine in prefrontal cortex and nucleus accumbens in phencyclidine model of schizophrenia.
Zuo D, Bzdega T, Olszewski RT, Moffett JR, Neale JH
The Journal of biological chemistry287(26)21773-82(2012 Jun)

Molecular identification of ?-citrylglutamate hydrolase as glutamate carboxypeptidase 3.
Collard F, Vertommen D, Constantinescu S, Buts L, Van Schaftingen E
The Journal of biological chemistry286(44)38220-30(2011 Nov)

Overview of homocysteine and folate metabolism. With special references to cardiovascular disease and neural tube defects.
Blom HJ, Smulders Y
Journal of inherited metabolic disease34(1)75-81(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NAAG peptidase inhibition has a role in release of glutamate and dopamine in prefrontal cortex and nucleus accumbens in phencyclidine model of schizophrenia
Title: Effects of N-acetylaspartylglutamate (NAAG) peptidase inhibition on release of glutamate and dopamine in prefrontal cortex and nucleus accumbens in phencyclidine model of schizophrenia.

Tissues such as prostate and testes exhibit different GCPII expression levels among the species studied (human, rats, swine).
Title: Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs.

role of glutamate carboxypeptidase II inhibition in preventing programmed cell death in neurons exposed to high glucose; this neuroprotection is mediated through the mGluR3 receptor, most likely by increasing the extracellular NAAG concentration
Title: Protection against glucose-induced neuronal death by NAAG and GCP II inhibition is regulated by mGluR3.

The following Folh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Folh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11522	NM_057185.2 Latest version!	Rattus norvegicus folate hydrolase 1 (Folh1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Folh1 ( NM_057185.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_476533.1 Rattus norvegicus folate hydrolase 1 (Folh1), mRNA.

Price & Availability↑

CloneID	ORa11522
Clone ID Related Accession (Same CDS sequence)	NM_057185.2
Accession Version	NM_057185.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	glutamate carboxypeptidase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF513486.2. On Feb 9, 2004 this sequence version replaced NM_057185.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF513486.2, U75973.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGTGGAACG CTCAGCAGGA CAGCGACTCC GCGGAGGCCC TGGGACGCCG CCAACGCTGG 
TTCTGTGCTG GAACACTGGT GCTGGCTTTC ACTGGAACCT TCATCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCCTC CAATGATTCT ACTAGCAGTG TTTCCTATCC TGGCATGAAG 
AAGGCATTTT TGCAAGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ATTTGGCAGG GACACAACAC AATTTTGAGC TTGCAAAGCA AATTCATGCC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCTG ATTATGATGT CCTGTTGTCC 
TACCCAAATA AGACCCATCC CAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTAGCTGA ACTGTCACCC CCGGGATATG AGAACATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GGGATCTAGT ATATGTTAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGTCA TGAAGATCAA TTGTTCTGGG 
AAGATTGTCA TCGCCAGATA TGGGCAAGTG TTCAGAGGAA ATAAGGTTAA AAATGCTCAG 
CTGGCAGGTG CAAAAGGAAT CATTCTGTAC TCAGACCCTG CTGATTACTT TGTTCCTGGG 
GTGAAGTCCT ATCCAGATGG TTGGAACCTC CCTGGAGGTG GTGTTCAGCG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGATCCCCTC ACACCAGGTT ATCCAGCAAA TGAATATGCC 
TACAGGCATG AATTCACAGA AGCTGTCGGT CTTCCAAGTA TTCCTGTCCA TCCAATTGGA 
TATGATGATG CACAGAAACT ATTAGAACAT ATGGGTGGCT CCGCACCCCC TGACAGCAGC 
TGGAAGGGAG GACTAAAAGT GCCTTACAAC GTGGGACCTG GCTTTGCTGG AAACTTTTCA 
AAACAAAAGG TCAAGCTGCA TATTCACTCC TACAATAAAG TGACAAGAAT CTACAATGTT 
ATTGGTACCC TCAAAGGAGC TGTGGAACCA GACAGATATG TTATTCTTGG AGGGCACAGA 
GATGCTTGGG TGTTTGGTGG CATTGACCCT CAGAGTGGAG CAGCTGTTGT TCATGAAATT 
GTCAGGACCT TTGGAACTCT GAAGAAGAAA GGTTGGAGGC CTAGAAGAAC TATTTTGTTT 
GCAAGCTGGG ATGCTGAAGA ATTTGGCCTT CTTGGTTCTA CTGAGTGGGC AGAGGAACAT 
TCAAGACTCT TACAAGAACG TGGTGTGGCT TATATCAACG CTGATTCTTC CATAGAAGGA 
AACTACACTC TCAGAGTTGA TTGCACACCA CTGATGCACA GCTTAGTATA CAACCTAACA 
AAAGAGCTGC CAAGCCCGGA TGAAGGCTTT GAAGGCAAAT CTCTTTATGA CAGCTGGAAA 
GAAAAAAGTC CTTCAACTGA GTTCATTGGA ATGCCAAGGA TTAGCAAGCT GGGGTCTGGC 
AATGATTTTG AAGTGTTTTT CCAAAGACTT GGAATTGCTT CAGGCAGAGC CCGGTATACT 
AAAAATTGGA AAAACAACAA AGTCAGCAGC TATCCTCTCT ATCACAGTGT CTATGAAACA 
TACGAATTGG TAGAAAAATT CTATGATCCG ACATTTAAAT ATCACCTCAC TGTGGCCCAG 
GTTCGAGGAG CAATGGTATT TGAATTGGCC AATTCTATAG TGCTTCCCTT TGACTGCCAA 
AGTTATGCTG TAGCTCTGAA GAAACATGCT GAGACTATCT ACAACATTTC AATGAATCAT 
CCACAGGAGA TGAAGGCGTA CATGATATCA TTTGATTCGC TGTTTTCTGC AGTAAATAAT 
TTTACAGATG TTGCATCCAA GTTCAATCAG AGACTGCAAG ACTTAGACAA GAGCAACCCC 
ATATTACTGA GAATTTTGAA TGATCAGCTA ATGTATCTGG AACGTGCATT CATTGATCCT 
TTAGGATTAC CAGGAAGGCC TTTCTACAGG CATATCATCT ATGCTCCAAG CAGCCACAAC 
AAGTATGCAG GAGAATCATT CCCAGGGATT TATGATGCCC TTTTTGATAT TAATAACAAA 
GTCGACACTT CTAAGGCCTG GAGAGAAGTG AAGAGACAGA TTTCTATTGC AGCCTTCACA 
GTGCAAGCTG CAGCAGAGAC TCTGAGAGAA GTAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476533.1
CDS	66..2324
Translation	MWNAQQDSDSAEALGRRQRWFCAGTLVLAFTGTFIIGFLFGWFIKPSNDSTSSVSYPGMKKAFLQELKAENIKKFLYNFTRTPHLAGTQHNFELAKQIHAQWKEFGLDLVELSDYDVLLSYPNKTHPNYISIINEDGNEIFKTSLAELSPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLERVMKINCSGKIVIARYGQVFRGNKVKNAQLAGAKGIILYSDPADYFVPGVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEYAYRHEFTEAVGLPSIPVHPIGYDDAQKLLEHMGGSAPPDSSWKGGLKVPYNVGPGFAGNFSKQKVKLHIHSYNKVTRIYNVIGTLKGAVEPDRYVILGGHRDAWVFGGIDPQSGAAVVHEIVRTFGTLKKKGWRPRRTILFASWDAEEFGLLGSTEWAEEHSRLLQERGVAYINADSSIEGNYTLRVDCTPLMHSLVYNLTKELPSPDEGFEGKSLYDSWKEKSPSTEFIGMPRISKLGSGNDFEVFFQRLGIASGRARYTKNWKNNKVSSYPLYHSVYETYELVEKFYDPTFKYHLTVAQVRGAMVFELANSIVLPFDCQSYAVALKKHAETIYNISMNHPQEMKAYMISFDSLFSAVNNFTDVASKFNQRLQDLDKSNPILLRILNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDINNKVDTSKAWREVKRQISIAAFTVQAAAETLREVD

Target ORF information:

RefSeq Version	NM_057185.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus folate hydrolase 1 (Folh1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057185.2

ORF Insert Sequence:

ATGTGGAACG CTCAGCAGGA CAGCGACTCC GCGGAGGCCC TGGGACGCCG CCAACGCTGG 
TTCTGTGCTG GAACACTGGT GCTGGCTTTC ACTGGAACCT TCATCATTGG CTTCCTCTTT 
GGGTGGTTTA TAAAACCCTC CAATGATTCT ACTAGCAGTG TTTCCTATCC TGGCATGAAG 
AAGGCATTTT TGCAAGAATT GAAGGCTGAG AACATCAAAA AATTTTTATA CAATTTCACA 
CGGACACCAC ATTTGGCAGG GACACAACAC AATTTTGAGC TTGCAAAGCA AATTCATGCC 
CAGTGGAAAG AATTTGGCCT GGATTTGGTT GAGTTATCTG ATTATGATGT CCTGTTGTCC 
TACCCAAATA AGACCCATCC CAACTATATC TCAATAATTA ATGAAGATGG AAATGAGATT 
TTCAAAACAT CATTAGCTGA ACTGTCACCC CCGGGATATG AGAACATATC AGATGTAGTG 
CCACCATACA GTGCCTTCTC TCCACAAGGG ACACCAGAGG GGGATCTAGT ATATGTTAAC 
TATGCACGAA CTGAAGACTT CTTTAAACTG GAACGGGTCA TGAAGATCAA TTGTTCTGGG 
AAGATTGTCA TCGCCAGATA TGGGCAAGTG TTCAGAGGAA ATAAGGTTAA AAATGCTCAG 
CTGGCAGGTG CAAAAGGAAT CATTCTGTAC TCAGACCCTG CTGATTACTT TGTTCCTGGG 
GTGAAGTCCT ATCCAGATGG TTGGAACCTC CCTGGAGGTG GTGTTCAGCG TGGAAATGTC 
TTAAATCTTA ATGGTGCAGG TGATCCCCTC ACACCAGGTT ATCCAGCAAA TGAATATGCC 
TACAGGCATG AATTCACAGA AGCTGTCGGT CTTCCAAGTA TTCCTGTCCA TCCAATTGGA 
TATGATGATG CACAGAAACT ATTAGAACAT ATGGGTGGCT CCGCACCCCC TGACAGCAGC 
TGGAAGGGAG GACTAAAAGT GCCTTACAAC GTGGGACCTG GCTTTGCTGG AAACTTTTCA 
AAACAAAAGG TCAAGCTGCA TATTCACTCC TACAATAAAG TGACAAGAAT CTACAATGTT 
ATTGGTACCC TCAAAGGAGC TGTGGAACCA GACAGATATG TTATTCTTGG AGGGCACAGA 
GATGCTTGGG TGTTTGGTGG CATTGACCCT CAGAGTGGAG CAGCTGTTGT TCATGAAATT 
GTCAGGACCT TTGGAACTCT GAAGAAGAAA GGTTGGAGGC CTAGAAGAAC TATTTTGTTT 
GCAAGCTGGG ATGCTGAAGA ATTTGGCCTT CTTGGTTCTA CTGAGTGGGC AGAGGAACAT 
TCAAGACTCT TACAAGAACG TGGTGTGGCT TATATCAACG CTGATTCTTC CATAGAAGGA 
AACTACACTC TCAGAGTTGA TTGCACACCA CTGATGCACA GCTTAGTATA CAACCTAACA 
AAAGAGCTGC CAAGCCCGGA TGAAGGCTTT GAAGGCAAAT CTCTTTATGA CAGCTGGAAA 
GAAAAAAGTC CTTCAACTGA GTTCATTGGA ATGCCAAGGA TTAGCAAGCT GGGGTCTGGC 
AATGATTTTG AAGTGTTTTT CCAAAGACTT GGAATTGCTT CAGGCAGAGC CCGGTATACT 
AAAAATTGGA AAAACAACAA AGTCAGCAGC TATCCTCTCT ATCACAGTGT CTATGAAACA 
TACGAATTGG TAGAAAAATT CTATGATCCG ACATTTAAAT ATCACCTCAC TGTGGCCCAG 
GTTCGAGGAG CAATGGTATT TGAATTGGCC AATTCTATAG TGCTTCCCTT TGACTGCCAA 
AGTTATGCTG TAGCTCTGAA GAAACATGCT GAGACTATCT ACAACATTTC AATGAATCAT 
CCACAGGAGA TGAAGGCGTA CATGATATCA TTTGATTCGC TGTTTTCTGC AGTAAATAAT 
TTTACAGATG TTGCATCCAA GTTCAATCAG AGACTGCAAG ACTTAGACAA GAGCAACCCC 
ATATTACTGA GAATTTTGAA TGATCAGCTA ATGTATCTGG AACGTGCATT CATTGATCCT 
TTAGGATTAC CAGGAAGGCC TTTCTACAGG CATATCATCT ATGCTCCAAG CAGCCACAAC 
AAGTATGCAG GAGAATCATT CCCAGGGATT TATGATGCCC TTTTTGATAT TAATAACAAA 
GTCGACACTT CTAAGGCCTG GAGAGAAGTG AAGAGACAGA TTTCTATTGC AGCCTTCACA 
GTGCAAGCTG CAGCAGAGAC TCTGAGAGAA GTAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural and biochemical characterization of the folyl-poly-?-l-glutamate hydrolyzing activity of human glutamate carboxypeptidase II.
The FEBS journal281(14)3228-42(2014 Jul)
Navr?til M,Pt??ek J,??cha P,Starkov? J,Lubkowski J,Ba?inka C,Konvalinka J

Effects of N-acetylaspartylglutamate (NAAG) peptidase inhibition on release of glutamate and dopamine in prefrontal cortex and nucleus accumbens in phencyclidine model of schizophrenia.
The Journal of biological chemistry287(26)21773-82(2012 Jun)
Zuo D,Bzdega T,Olszewski RT,Moffett JR,Neale JH

Molecular identification of ?-citrylglutamate hydrolase as glutamate carboxypeptidase 3.
The Journal of biological chemistry286(44)38220-30(2011 Nov)
Collard F,Vertommen D,Constantinescu S,Buts L,Van Schaftingen E

Overview of homocysteine and folate metabolism. With special references to cardiovascular disease and neural tube defects.
Journal of inherited metabolic disease34(1)75-81(2011 Feb)
Blom HJ,Smulders Y

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Folh1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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