Pdlim3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdlim3
Entrez Gene ID	53318
Full Name	PDZ and LIM domain 3
Synonyms	AI463105,ALP,Actn2lp
General protein information	Preferred Names PDZ and LIM domain 3 Names PDZ and LIM domain protein 3 actinin alpha 2 associated LIM protein actinin-associated LIM protein alpha-actinin-2-associated LIM protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 B1.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006509465.2	XP_006509528.1	PDZ and LIM domain protein 3 isoform X1
NM_016798.4	NP_058078.1	PDZ and LIM domain protein 3 isoform 2
NM_001374652.1	NP_001361581.1	PDZ and LIM domain protein 3 isoform 1
NM_016798.3	NP_058078.1	PDZ and LIM domain protein 3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PDLIM3	NP_001001764.1
Danio rerio (zebrafish)	pdlim3b	NP_001036183.1
Mus musculus (house mouse)	Pdlim3	NP_058078.1
Bos taurus (cattle)	PDLIM3	NP_001029818.1

Gene Ontology
Bibliography

Related articles in PubMed

Animal Models of Congenital Cardiomyopathies Associated With Mutations in Z-Line Proteins.
Bang ML
Journal of cellular physiology232(1)38-52(2017 Jan)

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Lewandowski JP, Du F, Zhang S, Powell MB, Falkenstein KN, Ji H, Vokes SA
Developmental biology406(1)92-103(2015 Oct)

Integrative genomic approach identifies multiple genes involved in cardiac collagen deposition.
Lodder EM, Scicluna BP, Beekman L, Arends D, Moerland PD, Tanck MW, Adriaens ME, Bezzina CR
Circulation. Cardiovascular genetics7(6)790-8(2014 Dec)

Radial glia require PDGFD-PDGFR? signalling in human but not mouse neocortex.
Lui JH, Nowakowski TJ, Pollen AA, Javaherian A, Kriegstein AR, Oldham MC
Nature515(7526)264-8(2014 Nov)

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Lumayag S, Haldin CE, Corbett NJ, Wahlin KJ, Cowan C, Turturro S, Larsen PE, Kovacs B, Witmer PD, Valle D, Zack DJ, Nicholson DA, Xu S
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

upon induction of muscle differentiation a nuclear translocation of smALP from the cytoplasm to the nucleus of C2C12 myoblasts, concomitant to an up-regulation of the protein expression, occurs in parallel with the nuclear accumulation of myogenin.
Title: Nuclear translocation of the cytoskeleton-associated protein, smALP, upon induction of skeletal muscle differentiation.

characterized the internal interaction site of ALP by NMR
Title: Backbone NMR assignment of the internal interaction site of ALP.

critical role of ALP on muscle differentiation, likely via cytoskeletal regulation of SRF
Title: The cytoskeleton-associated PDZ-LIM protein, ALP, acts on serum response factor activity to regulate muscle differentiation.

ZASP-like motif in actinin-associated LIM protein is required for interaction with the alpha-actinin rod and for targeting to the muscle Z-line
Title: The ZASP-like motif in actinin-associated LIM protein is required for interaction with the alpha-actinin rod and for targeting to the muscle Z-line.

The following Pdlim3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdlim3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu40872	XM_006509465.2 Latest version!	Mus musculus PDZ and LIM domain 3 (Pdlim3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu10643	NM_016798.4 Latest version!	Mus musculus PDZ and LIM domain 3 (Pdlim3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
OMu40872	NM_001374652.1 Latest version!	Mus musculus PDZ and LIM domain 3 (Pdlim3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu10643	NM_016798.3	Mus musculus PDZ and LIM domain 3 (Pdlim3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdlim3 ( XM_006509465.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509528.1 Mus musculus PDZ and LIM domain 3 (Pdlim3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40872
Clone ID Related Accession (Same CDS sequence)	XM_006509465.2 , NM_001374652.1
Accession Version	XM_006509465.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	PDZ and LIM domain protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509465.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCAGA ACGTAGTTCT CCCGGGTCCT GCGCCCTGGG GCTTCAGACT CTCTGGGGGC 
ATAGACTTCA ATCAGCCTTT GGTCATCACC AGGATCACTC CAGGAAGCAA AGCAGCAGCT 
GCCAACCTGT GTCCTGGAGA TGTCATCCTA GCTATTGATG GCTTTGGTAC GGAGTCTATG 
ACTCATGCTG ATGCACAGGA CAGGATTAAA GCAGCGTCCT ACCAGTTGTG TCTCAAGATT 
GACAGGGCAG AAACTCGCTT ATGGTCCCCG CAGGTGTCTG AAGATGGAAA AGCACACCCC 
TTCAAAATCA ACTTAGAAGC TGAACCACAG GATGTGAACT ACTTTGAACA CAAGCACAAT 
ATTCGGCCCA AACCTTTCAT AATTCCAGGC CGAACCAGTG GCTGCAGCAC TCCTTCCGGT 
GTTGACTGTG GCAGTGGACG TAGCACCCCT TCTTCCGTCA GTACCGTTAG TACAATCTGC 
CCGGGTGACT TGAAAGTTGC TGCTAAGATG GCCCCTAACA TTCCTTTGGA AATGGAACTT 
CCGGGCGTGA AGATCGTACA CGCTCAGTTT AACACGCCTA TGCAGTTGTA CTCAGATGAC 
AATATTATGG AAACACTTCA GGGTCAGGTT GCCACAGCTC TAGGGGAGAC ATCCTCCATG 
AGTGAACCCA CAGCTTCAGT GCCCCCTCAG TCGGACGTGT ACCGCATGCT CCATGACAAC 
CGGGATGACC CAGCCGCTCC TCGCCAGTCG GGCTCCTTCA GGGTGCTGCA GGATCTGGTG 
AATGACGGGC CTGATGACCG TCCGGCTGGA ACTCGAAGCG TGAGGGCTCC GGTTACGAAA 
GTCCACGGTG GGGCTGGCAG TGCCCAGAGG ATGCCGCTCT GTGACAAGTG TGGGAGTGGT 
ATTGTAGGTG CTGTCGTCAA GGCCCGGGAT AAGTACCGGC ACCCCGAGTG CTTTGTGTGT 
GCCGACTGTA ACCTCAACCT CAAGCAGAAA GGCTACTTCT TCGTGGAGGG GGAGCTGTAT 
TGTGAGACGC ACGCGAGAGC CCGCACGAGG CCCCCAGAGG GCTACGACAC CGTCACTCTG 
TACCCCAAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509528.1
CDS	129..1223
Translation	MPQNVVLPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAASYQLCLKIDRAETRLWSPQVSEDGKAHPFKINLEAEPQDVNYFEHKHNIRPKPFIIPGRTSGCSTPSGVDCGSGRSTPSSVSTVSTICPGDLKVAAKMAPNIPLEMELPGVKIVHAQFNTPMQLYSDDNIMETLQGQVATALGETSSMSEPTASVPPQSDVYRMLHDNRDDPAAPRQSGSFRVLQDLVNDGPDDRPAGTRSVRAPVTKVHGGAGSAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFVEGELYCETHARARTRPPEGYDTVTLYPKA

Target ORF information:

RefSeq Version	XM_006509465.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PDZ and LIM domain 3 (Pdlim3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509465.2

ORF Insert Sequence:

ATGCCCCAGA ACGTAGTTCT CCCGGGTCCT GCGCCCTGGG GCTTCAGACT CTCTGGGGGC 
ATAGACTTCA ATCAGCCTTT GGTCATCACC AGGATCACTC CAGGAAGCAA AGCAGCAGCT 
GCCAACCTGT GTCCTGGAGA TGTCATCCTA GCTATTGATG GCTTTGGTAC GGAGTCTATG 
ACTCATGCTG ATGCACAGGA CAGGATTAAA GCAGCGTCCT ACCAGTTGTG TCTCAAGATT 
GACAGGGCAG AAACTCGCTT ATGGTCCCCG CAGGTGTCTG AAGATGGAAA AGCACACCCC 
TTCAAAATCA ACTTAGAAGC TGAACCACAG GATGTGAACT ACTTTGAACA CAAGCACAAT 
ATTCGGCCCA AACCTTTCAT AATTCCAGGC CGAACCAGTG GCTGCAGCAC TCCTTCCGGT 
GTTGACTGTG GCAGTGGACG TAGCACCCCT TCTTCCGTCA GTACCGTTAG TACAATCTGC 
CCGGGTGACT TGAAAGTTGC TGCTAAGATG GCCCCTAACA TTCCTTTGGA AATGGAACTT 
CCGGGCGTGA AGATCGTACA CGCTCAGTTT AACACGCCTA TGCAGTTGTA CTCAGATGAC 
AATATTATGG AAACACTTCA GGGTCAGGTT GCCACAGCTC TAGGGGAGAC ATCCTCCATG 
AGTGAACCCA CAGCTTCAGT GCCCCCTCAG TCGGACGTGT ACCGCATGCT CCATGACAAC 
CGGGATGACC CAGCCGCTCC TCGCCAGTCG GGCTCCTTCA GGGTGCTGCA GGATCTGGTG 
AATGACGGGC CTGATGACCG TCCGGCTGGA ACTCGAAGCG TGAGGGCTCC GGTTACGAAA 
GTCCACGGTG GGGCTGGCAG TGCCCAGAGG ATGCCGCTCT GTGACAAGTG TGGGAGTGGT 
ATTGTAGGTG CTGTCGTCAA GGCCCGGGAT AAGTACCGGC ACCCCGAGTG CTTTGTGTGT 
GCCGACTGTA ACCTCAACCT CAAGCAGAAA GGCTACTTCT TCGTGGAGGG GGAGCTGTAT 
TGTGAGACGC ACGCGAGAGC CCGCACGAGG CCCCCAGAGG GCTACGACAC CGTCACTCTG 
TACCCCAAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdlim3 ( NM_016798.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058078.1 Mus musculus PDZ and LIM domain 3 (Pdlim3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu10643
Clone ID Related Accession (Same CDS sequence)	NM_016798.4 , NM_016798.3
Accession Version	NM_016798.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	PDZ and LIM domain protein 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113469.9 and AC114558.18. On Jun 12, 2018 this sequence version replaced NM_016798.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK078421.1, AK016447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCAGA ACGTAGTTCT CCCGGGTCCT GCGCCCTGGG GCTTCAGACT CTCTGGGGGC 
ATAGACTTCA ATCAGCCTTT GGTCATCACC AGGATCACTC CAGGAAGCAA AGCAGCAGCT 
GCCAACCTGT GTCCTGGAGA TGTCATCCTA GCTATTGATG GCTTTGGTAC GGAGTCTATG 
ACTCATGCTG ATGCACAGGA CAGGATTAAA GCAGCGTCCT ACCAGTTGTG TCTCAAGATT 
GACAGGGCAG AAACTCGCTT ATGGTCCCCG CAGGTGTCTG AAGATGGAAA AGCACACCCC 
TTCAAAATCA ACTTAGAAGC TGAACCACAG GAATTCAAAC CCATTGGTAC CGCGCACAAC 
AGAAGGGCCC AGCCTTTCGT AGCAGCAGCA AACATTGATG ACAAAAGACA GGTAGTGAGC 
GCTTCCTATA ACTCACCAAT TGGGCTCTAT TCAACTAGCA ATATCCAAGA CGCGCTCCAC 
GGACAGCTAC GGGGTCTCAT CCCCGGATCA CTGCAAAATG AACCCACAGC TTCAGTGCCC 
CCTCAGTCGG ACGTGTACCG CATGCTCCAT GACAACCGGG ATGACCCAGC CGCTCCTCGC 
CAGTCGGGCT CCTTCAGGGT GCTGCAGGAT CTGGTGAATG ACGGGCCTGA TGACCGTCCG 
GCTGGAACTC GAAGCGTGAG GGCTCCGGTT ACGAAAGTCC ACGGTGGGGC TGGCAGTGCC 
CAGAGGATGC CGCTCTGTGA CAAGTGTGGG AGTGGTATTG TAGGTGCTGT CGTCAAGGCC 
CGGGATAAGT ACCGGCACCC CGAGTGCTTT GTGTGTGCCG ACTGTAACCT CAACCTCAAG 
CAGAAAGGCT ACTTCTTCGT GGAGGGGGAG CTGTATTGTG AGACGCACGC GAGAGCCCGC 
ACGAGGCCCC CAGAGGGCTA CGACACCGTC ACTCTGTACC CCAAAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058078.1
CDS	94..1044
Translation	MPQNVVLPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAASYQLCLKIDRAETRLWSPQVSEDGKAHPFKINLEAEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPGSLQNEPTASVPPQSDVYRMLHDNRDDPAAPRQSGSFRVLQDLVNDGPDDRPAGTRSVRAPVTKVHGGAGSAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFVEGELYCETHARARTRPPEGYDTVTLYPKA

Target ORF information:

RefSeq Version	NM_016798.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus PDZ and LIM domain 3 (Pdlim3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016798.4

ORF Insert Sequence:

ATGCCCCAGA ACGTAGTTCT CCCGGGTCCT GCGCCCTGGG GCTTCAGACT CTCTGGGGGC 
ATAGACTTCA ATCAGCCTTT GGTCATCACC AGGATCACTC CAGGAAGCAA AGCAGCAGCT 
GCCAACCTGT GTCCTGGAGA TGTCATCCTA GCTATTGATG GCTTTGGTAC GGAGTCTATG 
ACTCATGCTG ATGCACAGGA CAGGATTAAA GCAGCGTCCT ACCAGTTGTG TCTCAAGATT 
GACAGGGCAG AAACTCGCTT ATGGTCCCCG CAGGTGTCTG AAGATGGAAA AGCACACCCC 
TTCAAAATCA ACTTAGAAGC TGAACCACAG GAATTCAAAC CCATTGGTAC CGCGCACAAC 
AGAAGGGCCC AGCCTTTCGT AGCAGCAGCA AACATTGATG ACAAAAGACA GGTAGTGAGC 
GCTTCCTATA ACTCACCAAT TGGGCTCTAT TCAACTAGCA ATATCCAAGA CGCGCTCCAC 
GGACAGCTAC GGGGTCTCAT CCCCGGATCA CTGCAAAATG AACCCACAGC TTCAGTGCCC 
CCTCAGTCGG ACGTGTACCG CATGCTCCAT GACAACCGGG ATGACCCAGC CGCTCCTCGC 
CAGTCGGGCT CCTTCAGGGT GCTGCAGGAT CTGGTGAATG ACGGGCCTGA TGACCGTCCG 
GCTGGAACTC GAAGCGTGAG GGCTCCGGTT ACGAAAGTCC ACGGTGGGGC TGGCAGTGCC 
CAGAGGATGC CGCTCTGTGA CAAGTGTGGG AGTGGTATTG TAGGTGCTGT CGTCAAGGCC 
CGGGATAAGT ACCGGCACCC CGAGTGCTTT GTGTGTGCCG ACTGTAACCT CAACCTCAAG 
CAGAAAGGCT ACTTCTTCGT GGAGGGGGAG CTGTATTGTG AGACGCACGC GAGAGCCCGC 
ACGAGGCCCC CAGAGGGCTA CGACACCGTC ACTCTGTACC CCAAAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdlim3 ( NM_016798.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058078.1 Mus musculus PDZ and LIM domain 3 (Pdlim3), mRNA.

Price & Availability↑

CloneID	OMu10643
Clone ID Related Accession (Same CDS sequence)	NM_016798.4 , NM_016798.3
Accession Version	NM_016798.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-07
Organism	Mus musculus(house mouse)
Product	PDZ and LIM domain protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC113469.9 and AC114558.18. On Mar 21, 2007 this sequence version replaced NM_016798.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK078421.1, AK016447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCCCCAGA ACGTAGTTCT CCCGGGTCCT GCGCCCTGGG GCTTCAGACT CTCTGGGGGC 
ATAGACTTCA ATCAGCCTTT GGTCATCACC AGGATCACTC CAGGAAGCAA AGCAGCAGCT 
GCCAACCTGT GTCCTGGAGA TGTCATCCTA GCTATTGATG GCTTTGGTAC GGAGTCTATG 
ACTCATGCTG ATGCACAGGA CAGGATTAAA GCAGCGTCCT ACCAGTTGTG TCTCAAGATT 
GACAGGGCAG AAACTCGCTT ATGGTCCCCG CAGGTGTCTG AAGATGGAAA AGCACACCCC 
TTCAAAATCA ACTTAGAAGC TGAACCACAG GAATTCAAAC CCATTGGTAC CGCGCACAAC 
AGAAGGGCCC AGCCTTTCGT AGCAGCAGCA AACATTGATG ACAAAAGACA GGTAGTGAGC 
GCTTCCTATA ACTCACCAAT TGGGCTCTAT TCAACTAGCA ATATCCAAGA CGCGCTCCAC 
GGACAGCTAC GGGGTCTCAT CCCCGGATCA CTGCAAAATG AACCCACAGC TTCAGTGCCC 
CCTCAGTCGG ACGTGTACCG CATGCTCCAT GACAACCGGG ATGACCCAGC CGCTCCTCGC 
CAGTCGGGCT CCTTCAGGGT GCTGCAGGAT CTGGTGAATG ACGGGCCTGA TGACCGTCCG 
GCTGGAACTC GAAGCGTGAG GGCTCCGGTT ACGAAAGTCC ACGGTGGGGC TGGCAGTGCC 
CAGAGGATGC CGCTCTGTGA CAAGTGTGGG AGTGGTATTG TAGGTGCTGT CGTCAAGGCC 
CGGGATAAGT ACCGGCACCC CGAGTGCTTT GTGTGTGCCG ACTGTAACCT CAACCTCAAG 
CAGAAAGGCT ACTTCTTCGT GGAGGGGGAG CTGTATTGTG AGACGCACGC GAGAGCCCGC 
ACGAGGCCCC CAGAGGGCTA CGACACCGTC ACTCTGTACC CCAAAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058078.1
CDS	96..1046
Translation	MPQNVVLPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAASYQLCLKIDRAETRLWSPQVSEDGKAHPFKINLEAEPQEFKPIGTAHNRRAQPFVAAANIDDKRQVVSASYNSPIGLYSTSNIQDALHGQLRGLIPGSLQNEPTASVPPQSDVYRMLHDNRDDPAAPRQSGSFRVLQDLVNDGPDDRPAGTRSVRAPVTKVHGGAGSAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFVEGELYCETHARARTRPPEGYDTVTLYPKA

Target ORF information:

RefSeq Version	NM_016798.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus PDZ and LIM domain 3 (Pdlim3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016798.3

ORF Insert Sequence:

ATGCCCCAGA ACGTAGTTCT CCCGGGTCCT GCGCCCTGGG GCTTCAGACT CTCTGGGGGC 
ATAGACTTCA ATCAGCCTTT GGTCATCACC AGGATCACTC CAGGAAGCAA AGCAGCAGCT 
GCCAACCTGT GTCCTGGAGA TGTCATCCTA GCTATTGATG GCTTTGGTAC GGAGTCTATG 
ACTCATGCTG ATGCACAGGA CAGGATTAAA GCAGCGTCCT ACCAGTTGTG TCTCAAGATT 
GACAGGGCAG AAACTCGCTT ATGGTCCCCG CAGGTGTCTG AAGATGGAAA AGCACACCCC 
TTCAAAATCA ACTTAGAAGC TGAACCACAG GAATTCAAAC CCATTGGTAC CGCGCACAAC 
AGAAGGGCCC AGCCTTTCGT AGCAGCAGCA AACATTGATG ACAAAAGACA GGTAGTGAGC 
GCTTCCTATA ACTCACCAAT TGGGCTCTAT TCAACTAGCA ATATCCAAGA CGCGCTCCAC 
GGACAGCTAC GGGGTCTCAT CCCCGGATCA CTGCAAAATG AACCCACAGC TTCAGTGCCC 
CCTCAGTCGG ACGTGTACCG CATGCTCCAT GACAACCGGG ATGACCCAGC CGCTCCTCGC 
CAGTCGGGCT CCTTCAGGGT GCTGCAGGAT CTGGTGAATG ACGGGCCTGA TGACCGTCCG 
GCTGGAACTC GAAGCGTGAG GGCTCCGGTT ACGAAAGTCC ACGGTGGGGC TGGCAGTGCC 
CAGAGGATGC CGCTCTGTGA CAAGTGTGGG AGTGGTATTG TAGGTGCTGT CGTCAAGGCC 
CGGGATAAGT ACCGGCACCC CGAGTGCTTT GTGTGTGCCG ACTGTAACCT CAACCTCAAG 
CAGAAAGGCT ACTTCTTCGT GGAGGGGGAG CTGTATTGTG AGACGCACGC GAGAGCCCGC 
ACGAGGCCCC CAGAGGGCTA CGACACCGTC ACTCTGTACC CCAAAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdlim3 ( NM_001374652.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001361581.1 Mus musculus PDZ and LIM domain 3 (Pdlim3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu40872
Clone ID Related Accession (Same CDS sequence)	XM_006509465.2 , NM_001374652.1
Accession Version	NM_001374652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Mus musculus(house mouse)
Product	PDZ and LIM domain protein 3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113469.9 and AC114558.18. On Oct 8, 2019 this sequence version replaced XM_006509465.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189673.119238.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END##

ATGCCCCAGA ACGTAGTTCT CCCGGGTCCT GCGCCCTGGG GCTTCAGACT CTCTGGGGGC 
ATAGACTTCA ATCAGCCTTT GGTCATCACC AGGATCACTC CAGGAAGCAA AGCAGCAGCT 
GCCAACCTGT GTCCTGGAGA TGTCATCCTA GCTATTGATG GCTTTGGTAC GGAGTCTATG 
ACTCATGCTG ATGCACAGGA CAGGATTAAA GCAGCGTCCT ACCAGTTGTG TCTCAAGATT 
GACAGGGCAG AAACTCGCTT ATGGTCCCCG CAGGTGTCTG AAGATGGAAA AGCACACCCC 
TTCAAAATCA ACTTAGAAGC TGAACCACAG GATGTGAACT ACTTTGAACA CAAGCACAAT 
ATTCGGCCCA AACCTTTCAT AATTCCAGGC CGAACCAGTG GCTGCAGCAC TCCTTCCGGT 
GTTGACTGTG GCAGTGGACG TAGCACCCCT TCTTCCGTCA GTACCGTTAG TACAATCTGC 
CCGGGTGACT TGAAAGTTGC TGCTAAGATG GCCCCTAACA TTCCTTTGGA AATGGAACTT 
CCGGGCGTGA AGATCGTACA CGCTCAGTTT AACACGCCTA TGCAGTTGTA CTCAGATGAC 
AATATTATGG AAACACTTCA GGGTCAGGTT GCCACAGCTC TAGGGGAGAC ATCCTCCATG 
AGTGAACCCA CAGCTTCAGT GCCCCCTCAG TCGGACGTGT ACCGCATGCT CCATGACAAC 
CGGGATGACC CAGCCGCTCC TCGCCAGTCG GGCTCCTTCA GGGTGCTGCA GGATCTGGTG 
AATGACGGGC CTGATGACCG TCCGGCTGGA ACTCGAAGCG TGAGGGCTCC GGTTACGAAA 
GTCCACGGTG GGGCTGGCAG TGCCCAGAGG ATGCCGCTCT GTGACAAGTG TGGGAGTGGT 
ATTGTAGGTG CTGTCGTCAA GGCCCGGGAT AAGTACCGGC ACCCCGAGTG CTTTGTGTGT 
GCCGACTGTA ACCTCAACCT CAAGCAGAAA GGCTACTTCT TCGTGGAGGG GGAGCTGTAT 
TGTGAGACGC ACGCGAGAGC CCGCACGAGG CCCCCAGAGG GCTACGACAC CGTCACTCTG 
TACCCCAAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361581.1
CDS	94..1188
Translation	MPQNVVLPGPAPWGFRLSGGIDFNQPLVITRITPGSKAAAANLCPGDVILAIDGFGTESMTHADAQDRIKAASYQLCLKIDRAETRLWSPQVSEDGKAHPFKINLEAEPQDVNYFEHKHNIRPKPFIIPGRTSGCSTPSGVDCGSGRSTPSSVSTVSTICPGDLKVAAKMAPNIPLEMELPGVKIVHAQFNTPMQLYSDDNIMETLQGQVATALGETSSMSEPTASVPPQSDVYRMLHDNRDDPAAPRQSGSFRVLQDLVNDGPDDRPAGTRSVRAPVTKVHGGAGSAQRMPLCDKCGSGIVGAVVKARDKYRHPECFVCADCNLNLKQKGYFFVEGELYCETHARARTRPPEGYDTVTLYPKA

Target ORF information:

RefSeq Version	NM_001374652.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PDZ and LIM domain 3 (Pdlim3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374652.1

ORF Insert Sequence:

ATGCCCCAGA ACGTAGTTCT CCCGGGTCCT GCGCCCTGGG GCTTCAGACT CTCTGGGGGC 
ATAGACTTCA ATCAGCCTTT GGTCATCACC AGGATCACTC CAGGAAGCAA AGCAGCAGCT 
GCCAACCTGT GTCCTGGAGA TGTCATCCTA GCTATTGATG GCTTTGGTAC GGAGTCTATG 
ACTCATGCTG ATGCACAGGA CAGGATTAAA GCAGCGTCCT ACCAGTTGTG TCTCAAGATT 
GACAGGGCAG AAACTCGCTT ATGGTCCCCG CAGGTGTCTG AAGATGGAAA AGCACACCCC 
TTCAAAATCA ACTTAGAAGC TGAACCACAG GATGTGAACT ACTTTGAACA CAAGCACAAT 
ATTCGGCCCA AACCTTTCAT AATTCCAGGC CGAACCAGTG GCTGCAGCAC TCCTTCCGGT 
GTTGACTGTG GCAGTGGACG TAGCACCCCT TCTTCCGTCA GTACCGTTAG TACAATCTGC 
CCGGGTGACT TGAAAGTTGC TGCTAAGATG GCCCCTAACA TTCCTTTGGA AATGGAACTT 
CCGGGCGTGA AGATCGTACA CGCTCAGTTT AACACGCCTA TGCAGTTGTA CTCAGATGAC 
AATATTATGG AAACACTTCA GGGTCAGGTT GCCACAGCTC TAGGGGAGAC ATCCTCCATG 
AGTGAACCCA CAGCTTCAGT GCCCCCTCAG TCGGACGTGT ACCGCATGCT CCATGACAAC 
CGGGATGACC CAGCCGCTCC TCGCCAGTCG GGCTCCTTCA GGGTGCTGCA GGATCTGGTG 
AATGACGGGC CTGATGACCG TCCGGCTGGA ACTCGAAGCG TGAGGGCTCC GGTTACGAAA 
GTCCACGGTG GGGCTGGCAG TGCCCAGAGG ATGCCGCTCT GTGACAAGTG TGGGAGTGGT 
ATTGTAGGTG CTGTCGTCAA GGCCCGGGAT AAGTACCGGC ACCCCGAGTG CTTTGTGTGT 
GCCGACTGTA ACCTCAACCT CAAGCAGAAA GGCTACTTCT TCGTGGAGGG GGAGCTGTAT 
TGTGAGACGC ACGCGAGAGC CCGCACGAGG CCCCCAGAGG GCTACGACAC CGTCACTCTG 
TACCCCAAAG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Animal Models of Congenital Cardiomyopathies Associated With Mutations in Z-Line Proteins.
Journal of cellular physiology232(1)38-52(2017 Jan)
Bang ML

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Developmental biology406(1)92-103(2015 Oct)
Lewandowski JP,Du F,Zhang S,Powell MB,Falkenstein KN,Ji H,Vokes SA

Integrative genomic approach identifies multiple genes involved in cardiac collagen deposition.
Circulation. Cardiovascular genetics7(6)790-8(2014 Dec)
Lodder EM,Scicluna BP,Beekman L,Arends D,Moerland PD,Tanck MW,Adriaens ME,Bezzina CR

Radial glia require PDGFD-PDGFR? signalling in human but not mouse neocortex.
Nature515(7526)264-8(2014 Nov)
Lui JH,Nowakowski TJ,Pollen AA,Javaherian A,Kriegstein AR,Oldham MC

Inactivation of the microRNA-183/96/182 cluster results in syndromic retinal degeneration.
Proceedings of the National Academy of Sciences of the United States of America110(6)E507-16(2013 Feb)
Lumayag S,Haldin CE,Corbett NJ,Wahlin KJ,Cowan C,Turturro S,Larsen PE,Kovacs B,Witmer PD,Valle D,Zack DJ,Nicholson DA,Xu S

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Pdlim3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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