TSPYL2 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	TSPYL2
Entrez Gene ID	52808
Full Name	TSPY-like 2
Synonyms	CDA1,CINAP,DENTT,DXBwg1396e,DXHXS1008E,E130307F10Rik
General protein information	Preferred Names TSPY-like 2 Names testis-specific Y-encoded-like protein 2 CASK interacting nucleosome assembly protein TSPY-like protein 2 cell division autoantigen 1 nucleolar protein differentially expressed nucleolar TGF-beta1 target nucleolar TGF-beta1 target protein
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: X Map Location: X F3\|X 68.46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001358369.1	NP_001345298.1	testis-specific Y-encoded-like protein 2 isoform c
NM_029836.3	NP_084112.1	testis-specific Y-encoded-like protein 2
NM_029836.4	NP_084112.1	testis-specific Y-encoded-like protein 2 isoform a
XM_006528908.3	XP_006528971.1	testis-specific Y-encoded-like protein 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TSPYL2	NP_071400.1
Pan troglodytes (chimpanzee)	TSPYL2	XP_001148762.2
	TSPYL2	XP_001085755.1
Bos taurus (cattle)	TSPYL2	XP_002700166.1
Canis lupus familiaris (dog)	TSPYL2	XP_005641432.1
Mus musculus (house mouse)	Tspyl2	NP_084112.1
Rattus norvegicus (Norway rat)	Tspyl2	NP_001178547.1

Gene Ontology
Bibliography

Related articles in PubMed

Diabetes Reduces Severity of Aortic Aneurysms Depending on the Presence of Cell Division Autoantigen 1 (CDA1).
Li J, Huynh P, Dai A, Wu T, Tu Y, Chow B, Kiriazis H, Du XJ, Bach LA, Wilkinson-Berka JL, Biros E, Walker P, Nataatmadja M, West M, Golledge J, Allen TJ, Cooper ME, Chai Z
Diabetes(2018 Jan)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The ATPase hCINAP regulates 18S rRNA processing and is essential for embryogenesis and tumour growth.
Bai D, Zhang J, Li T, Hang R, Liu Y, Tian Y, Huang D, Qu L, Cao X, Ji J, Zheng X
Nature communications712310(2016 Aug)

Tspyl2 Loss-of-Function Causes Neurodevelopmental Brain and Behavior Abnormalities in Mice.
Li Q, Chan SY, Wong KK, Wei R, Leung YO, Ding AY, Hui TC, Cheung C, Chua SE, Sham PC, Wu EX, McAlonan GM
Behavior genetics46(4)529-37(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that disrupting Tspyl2 gene expression leads to behavioral and brain morphological alterations that mirror a number of neurodevelopmental psychiatric traits.
Title: Tspyl2 Loss-of-Function Causes Neurodevelopmental Brain and Behavior Abnormalities in Mice.

Following infection with T. gondii, myotubes but not myoblasts or fibroblasts further upregulate the negative cell cycle regulator Tspyl2.
Title: Withdrawal of skeletal muscle cells from cell cycle progression triggers differentiation of Toxoplasma gondii towards the bradyzoite stage.

We conclude that TSPYL2 contributes to cognitive variability through regulating the expression of Grin2a and Grin2b.
Title: The nucleosome assembly protein TSPYL2 regulates the expression of NMDA receptor subunits GluN2A and GluN2B.

CDA1 deletion reduces but does not block renal TGF-beta signaling.
Title: Genetic deletion of cell division autoantigen 1 retards diabetes-associated renal injury.

TSPYL2 serves a biological role in maintaining the G1 checkpoint under stress condition.
Title: TSPYL2 is important for G1 checkpoint maintenance upon DNA damage.

The following TSPYL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSPYL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07541	NM_029836.4 Latest version!	Mus musculus TSPY-like 2 (Tspyl2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu56698	NM_001358369.1 Latest version!	Mus musculus TSPY-like 2 (Tspyl2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu56698	XM_006528908.3 Latest version!	Mus musculus TSPY-like 2 (Tspyl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu07541	NM_029836.3	Mus musculus TSPY-like 2 (Tspyl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSPYL2 ( NM_029836.3 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_084112.1 Mus musculus TSPY-like 2 (Tspyl2), mRNA.

Price & Availability↑

CloneID	OMu07541
Clone ID Related Accession (Same CDS sequence)	NM_029836.3 , NM_029836.4
Accession Version	NM_029836.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-27
Organism	Mus musculus(House mouse)
Product	testis-specific Y-encoded-like protein 2
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ066847.1, BY008032.1 and BC054393.1. On Feb 14, 2006 this sequence version replaced NM_029836.2. ##Evidence-Data-START## Transcript exon combination :: BC054393.1, BK000279.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGCC CGGACGAGGG GCCTCCGGCC AAGACCCCCC GCCTGAGCAG CTCGGAACCC 
CGTCAGCGCG ACCTGCCGCC CCCGCCACCC CCACCGCTCC AGCGACTGCC CCTGCCTCCA 
CCCCAGCAGC GTCCGAGACC CCAGGAAGAA ACCGAGGCTG CACAGGTGCT GGCTGACATG 
AGGGGGGTGG GCCCCACGCT GCCCCCACCA CTGCCCTATG TCATTCTTGA GGAAGGGGGG 
ATCCGCGCGT ATTTCACCCT GAGTGCTGAG AGTCCCGGAT GGGATCACGC AATGGAGTCA 
GGGTTCGGGG AGGCCCCTTC CACAGGGATC ATGGAAACGC TCCCATCTTC AGAAATCTCT 
GGGGGAAGCC TGGCAATTGA CTTTCAGGTT GCAGAGCCCA GCAGCCTCGG AGAGAAGGCC 
CTAGAAACCT GTAGCTTAGG AGGGTGGGGG CCCCAGATGT TAGTTGGTCC GAAGAGGAAG 
GAGGAGGCTA TCATAATAGT GGAAGATGAG GATGAGGATG ACAAGGAGAG TGTGAGGAGG 
CGGCAGCGTC GGAGGAGGAG GAGAAGGAAG CAGAGGAAGG CGAAGGAGAG CCGAGAGAGG 
AGTGCCCAGA GGATGGAGAG CATACTGCAG GCCCTGGAGA GCATTCAAAT GGACCTGGAG 
GCGGTGAACA TCAAGGCAGG CAAGGCCTTC TTGCGTCTCA AGCGCAAATT CATCCAGATG 
CGAAGGCCCT TTTTGGAGCG CAGAGACCTC ATCATCCAGC ATATCCCAGG CTTCTGGGTC 
AAAGCATTCC TCAACCACCC CAGAATTTCA ATCTTGATCA ACCAACGTGA CAGAGACATT 
TTCCGCTACT TGACCAATCT TCAGGTACAG GACCTCAGAC ATATCTCCAT GGGCTACAAA 
ATGAAGCTGT ACTTCCAGAC AAACCCATAC TTTACAAACA TGGTGATCGT CAAGGAGTTC 
CAGCGCAACC GCTCAGGCCG GTTGGTGTCT CATTCTACCC CAATACGTTG GCACCGGGGC 
CAGGAACCCC AGGCCTATAA TCGCAGGAGC CATGACACCA GAGAAAGCTT CTTCAACTGG 
TTTTCCAATC ACAGCCTCCC AGAAGCTGAC AGAATTGCTG AGATCATCAA GAACGACCTG 
TGGGTTAACC CAGTACGCTA CTATATGAGA CGAGGTGGCT ACAGATCAAG CAGAAAGAAG 
CAACACGGAA AGGAAAGAGC CAAGAATCAA TATGAAATGG TGATCATGGA AGATGCTCAT 
GACCATTATG CAATTGAAGA CATTCTCAGT GACATCTCAG AAATTGATGA GATCACTGAC 
AATGAGACCA TTCACGACAT CAAGATCTCT GACTTCATGG AGACCACCGA CTACTTCGAG 
ACTACTGATA ATGAGGTGAC CGATGCCAAT GAGAACCTGT GCGACAGTGA GAATCCTGAC 
CACAGTGAAG GCTACAACAC CAAGATCACT GACAACAAAG GGAGCGTTGC CGCAAATCCA 
GATGACAACA GTGATGACCC AGAGGAAAAG AATACCTATG ACAGTGAGGA TTCCAACAGC 
GAGAAAGCTG ATGGTGATAA CACAACCCTA CGTGACAACC AGCAGGTTAC TAATATCCAG 
GACAGCAGTG ATAGTGACAA TGGAGATGAG GGCAGTGATG ATGAAGATGA TGATGGTAAT 
GAGGGGGACA ATGAGGGCAG TGATGATGAT GATGATGACA ATGAGGGCAG TGATGATGAT 
GACAGGGACA TTAGATACTA TAAGAATGGC CCTGAAGTCT TTGACAAGGC TCTGGATAAC 
CGCACCAACC AGAATGACTA TGAGGAGGAG GTAGAACTCA TCTCTGAAGA CTCAGTTGAA 
GAAGAGGAAG AGACCAGTGA GGAAGCCAGC CAGCTAAGTG AGGACAGCTA TGAAGATGAG 
CGAATCTATG GGGAGGAAAG AAGTGAGGTG AACTCCGAAG ATTCTGATAT TCAGGAAGTG 
CTGCCGGTCC CAAAAGCTTG GGCCAGCCTG GGGAAGAAAG GGAAAATCGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084112.1
CDS	160..2193
Translation	MDRPDEGPPAKTPRLSSSEPRQRDLPPPPPPPLQRLPLPPPQQRPRPQEETEAAQVLADMRGVGPTLPPPLPYVILEEGGIRAYFTLSAESPGWDHAMESGFGEAPSTGIMETLPSSEISGGSLAIDFQVAEPSSLGEKALETCSLGGWGPQMLVGPKRKEEAIIIVEDEDEDDKESVRRRQRRRRRRRKQRKAKESRERSAQRMESILQALESIQMDLEAVNIKAGKAFLRLKRKFIQMRRPFLERRDLIIQHIPGFWVKAFLNHPRISILINQRDRDIFRYLTNLQVQDLRHISMGYKMKLYFQTNPYFTNMVIVKEFQRNRSGRLVSHSTPIRWHRGQEPQAYNRRSHDTRESFFNWFSNHSLPEADRIAEIIKNDLWVNPVRYYMRRGGYRSSRKKQHGKERAKNQYEMVIMEDAHDHYAIEDILSDISEIDEITDNETIHDIKISDFMETTDYFETTDNEVTDANENLCDSENPDHSEGYNTKITDNKGSVAANPDDNSDDPEEKNTYDSEDSNSEKADGDNTTLRDNQQVTNIQDSSDSDNGDEGSDDEDDDGNEGDNEGSDDDDDDNEGSDDDDRDIRYYKNGPEVFDKALDNRTNQNDYEEEVELISEDSVEEEEETSEEASQLSEDSYEDERIYGEERSEVNSEDSDIQEVLPVPKAWASLGKKGKIG

Target ORF information:

RefSeq Version	NM_029836.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus TSPY-like 2 (Tspyl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029836.3

ORF Insert Sequence:

ATGGACCGCC CGGACGAGGG GCCTCCGGCC AAGACCCCCC GCCTGAGCAG CTCGGAACCC 
CGTCAGCGCG ACCTGCCGCC CCCGCCACCC CCACCGCTCC AGCGACTGCC CCTGCCTCCA 
CCCCAGCAGC GTCCGAGACC CCAGGAAGAA ACCGAGGCTG CACAGGTGCT GGCTGACATG 
AGGGGGGTGG GCCCCACGCT GCCCCCACCA CTGCCCTATG TCATTCTTGA GGAAGGGGGG 
ATCCGCGCGT ATTTCACCCT GAGTGCTGAG AGTCCCGGAT GGGATCACGC AATGGAGTCA 
GGGTTCGGGG AGGCCCCTTC CACAGGGATC ATGGAAACGC TCCCATCTTC AGAAATCTCT 
GGGGGAAGCC TGGCAATTGA CTTTCAGGTT GCAGAGCCCA GCAGCCTCGG AGAGAAGGCC 
CTAGAAACCT GTAGCTTAGG AGGGTGGGGG CCCCAGATGT TAGTTGGTCC GAAGAGGAAG 
GAGGAGGCTA TCATAATAGT GGAAGATGAG GATGAGGATG ACAAGGAGAG TGTGAGGAGG 
CGGCAGCGTC GGAGGAGGAG GAGAAGGAAG CAGAGGAAGG CGAAGGAGAG CCGAGAGAGG 
AGTGCCCAGA GGATGGAGAG CATACTGCAG GCCCTGGAGA GCATTCAAAT GGACCTGGAG 
GCGGTGAACA TCAAGGCAGG CAAGGCCTTC TTGCGTCTCA AGCGCAAATT CATCCAGATG 
CGAAGGCCCT TTTTGGAGCG CAGAGACCTC ATCATCCAGC ATATCCCAGG CTTCTGGGTC 
AAAGCATTCC TCAACCACCC CAGAATTTCA ATCTTGATCA ACCAACGTGA CAGAGACATT 
TTCCGCTACT TGACCAATCT TCAGGTACAG GACCTCAGAC ATATCTCCAT GGGCTACAAA 
ATGAAGCTGT ACTTCCAGAC AAACCCATAC TTTACAAACA TGGTGATCGT CAAGGAGTTC 
CAGCGCAACC GCTCAGGCCG GTTGGTGTCT CATTCTACCC CAATACGTTG GCACCGGGGC 
CAGGAACCCC AGGCCTATAA TCGCAGGAGC CATGACACCA GAGAAAGCTT CTTCAACTGG 
TTTTCCAATC ACAGCCTCCC AGAAGCTGAC AGAATTGCTG AGATCATCAA GAACGACCTG 
TGGGTTAACC CAGTACGCTA CTATATGAGA CGAGGTGGCT ACAGATCAAG CAGAAAGAAG 
CAACACGGAA AGGAAAGAGC CAAGAATCAA TATGAAATGG TGATCATGGA AGATGCTCAT 
GACCATTATG CAATTGAAGA CATTCTCAGT GACATCTCAG AAATTGATGA GATCACTGAC 
AATGAGACCA TTCACGACAT CAAGATCTCT GACTTCATGG AGACCACCGA CTACTTCGAG 
ACTACTGATA ATGAGGTGAC CGATGCCAAT GAGAACCTGT GCGACAGTGA GAATCCTGAC 
CACAGTGAAG GCTACAACAC CAAGATCACT GACAACAAAG GGAGCGTTGC CGCAAATCCA 
GATGACAACA GTGATGACCC AGAGGAAAAG AATACCTATG ACAGTGAGGA TTCCAACAGC 
GAGAAAGCTG ATGGTGATAA CACAACCCTA CGTGACAACC AGCAGGTTAC TAATATCCAG 
GACAGCAGTG ATAGTGACAA TGGAGATGAG GGCAGTGATG ATGAAGATGA TGATGGTAAT 
GAGGGGGACA ATGAGGGCAG TGATGATGAT GATGATGACA ATGAGGGCAG TGATGATGAT 
GACAGGGACA TTAGATACTA TAAGAATGGC CCTGAAGTCT TTGACAAGGC TCTGGATAAC 
CGCACCAACC AGAATGACTA TGAGGAGGAG GTAGAACTCA TCTCTGAAGA CTCAGTTGAA 
GAAGAGGAAG AGACCAGTGA GGAAGCCAGC CAGCTAAGTG AGGACAGCTA TGAAGATGAG 
CGAATCTATG GGGAGGAAAG AAGTGAGGTG AACTCCGAAG ATTCTGATAT TCAGGAAGTG 
CTGCCGGTCC CAAAAGCTTG GGCCAGCCTG GGGAAGAAAG GGAAAATCGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSPYL2 ( NM_029836.4 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_084112.1 Mus musculus TSPY-like 2 (Tspyl2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07541
Clone ID Related Accession (Same CDS sequence)	NM_029836.3 , NM_029836.4
Accession Version	NM_029836.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	testis-specific Y-encoded-like protein 2 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ066847.1, BY008032.1, BC054393.1 and AW046203.1. On Dec 1, 2017 this sequence version replaced NM_029836.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK000279.1, BC054393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGCC CGGACGAGGG GCCTCCGGCC AAGACCCCCC GCCTGAGCAG CTCGGAACCC 
CGTCAGCGCG ACCTGCCGCC CCCGCCACCC CCACCGCTCC AGCGACTGCC CCTGCCTCCA 
CCCCAGCAGC GTCCGAGACC CCAGGAAGAA ACCGAGGCTG CACAGGTGCT GGCTGACATG 
AGGGGGGTGG GCCCCACGCT GCCCCCACCA CTGCCCTATG TCATTCTTGA GGAAGGGGGG 
ATCCGCGCGT ATTTCACCCT GAGTGCTGAG AGTCCCGGAT GGGATCACGC AATGGAGTCA 
GGGTTCGGGG AGGCCCCTTC CACAGGGATC ATGGAAACGC TCCCATCTTC AGAAATCTCT 
GGGGGAAGCC TGGCAATTGA CTTTCAGGTT GCAGAGCCCA GCAGCCTCGG AGAGAAGGCC 
CTAGAAACCT GTAGCTTAGG AGGGTGGGGG CCCCAGATGT TAGTTGGTCC GAAGAGGAAG 
GAGGAGGCTA TCATAATAGT GGAAGATGAG GATGAGGATG ACAAGGAGAG TGTGAGGAGG 
CGGCAGCGTC GGAGGAGGAG GAGAAGGAAG CAGAGGAAGG CGAAGGAGAG CCGAGAGAGG 
AGTGCCCAGA GGATGGAGAG CATACTGCAG GCCCTGGAGA GCATTCAAAT GGACCTGGAG 
GCGGTGAACA TCAAGGCAGG CAAGGCCTTC TTGCGTCTCA AGCGCAAATT CATCCAGATG 
CGAAGGCCCT TTTTGGAGCG CAGAGACCTC ATCATCCAGC ATATCCCAGG CTTCTGGGTC 
AAAGCATTCC TCAACCACCC CAGAATTTCA ATCTTGATCA ACCAACGTGA CAGAGACATT 
TTCCGCTACT TGACCAATCT TCAGGTACAG GACCTCAGAC ATATCTCCAT GGGCTACAAA 
ATGAAGCTGT ACTTCCAGAC AAACCCATAC TTTACAAACA TGGTGATCGT CAAGGAGTTC 
CAGCGCAACC GCTCAGGCCG GTTGGTGTCT CATTCTACCC CAATACGTTG GCACCGGGGC 
CAGGAACCCC AGGCCTATAA TCGCAGGAGC CATGACACCA GAGAAAGCTT CTTCAACTGG 
TTTTCCAATC ACAGCCTCCC AGAAGCTGAC AGAATTGCTG AGATCATCAA GAACGACCTG 
TGGGTTAACC CAGTACGCTA CTATATGAGA CGAGGTGGCT ACAGATCAAG CAGAAAGAAG 
CAACACGGAA AGGAAAGAGC CAAGAATCAA TATGAAATGG TGATCATGGA AGATGCTCAT 
GACCATTATG CAATTGAAGA CATTCTCAGT GACATCTCAG AAATTGATGA GATCACTGAC 
AATGAGACCA TTCACGACAT CAAGATCTCT GACTTCATGG AGACCACCGA CTACTTCGAG 
ACTACTGATA ATGAGGTGAC CGATGCCAAT GAGAACCTGT GCGACAGTGA GAATCCTGAC 
CACAGTGAAG GCTACAACAC CAAGATCACT GACAACAAAG GGAGCGTTGC CGCAAATCCA 
GATGACAACA GTGATGACCC AGAGGAAAAG AATACCTATG ACAGTGAGGA TTCCAACAGC 
GAGAAAGCTG ATGGTGATAA CACAACCCTA CGTGACAACC AGCAGGTTAC TAATATCCAG 
GACAGCAGTG ATAGTGACAA TGGAGATGAG GGCAGTGATG ATGAAGATGA TGATGGTAAT 
GAGGGGGACA ATGAGGGCAG TGATGATGAT GATGATGACA ATGAGGGCAG TGATGATGAT 
GACAGGGACA TTAGATACTA TAAGAATGGC CCTGAAGTCT TTGACAAGGC TCTGGATAAC 
CGCACCAACC AGAATGACTA TGAGGAGGAG GTAGAACTCA TCTCTGAAGA CTCAGTTGAA 
GAAGAGGAAG AGACCAGTGA GGAAGCCAGC CAGCTAAGTG AGGACAGCTA TGAAGATGAG 
CGAATCTATG GGGAGGAAAG AAGTGAGGTG AACTCCGAAG ATTCTGATAT TCAGGAAGTG 
CTGCCGGTCC CAAAAGCTTG GGCCAGCCTG GGGAAGAAAG GGAAAATCGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084112.1
CDS	160..2193
Translation	MDRPDEGPPAKTPRLSSSEPRQRDLPPPPPPPLQRLPLPPPQQRPRPQEETEAAQVLADMRGVGPTLPPPLPYVILEEGGIRAYFTLSAESPGWDHAMESGFGEAPSTGIMETLPSSEISGGSLAIDFQVAEPSSLGEKALETCSLGGWGPQMLVGPKRKEEAIIIVEDEDEDDKESVRRRQRRRRRRRKQRKAKESRERSAQRMESILQALESIQMDLEAVNIKAGKAFLRLKRKFIQMRRPFLERRDLIIQHIPGFWVKAFLNHPRISILINQRDRDIFRYLTNLQVQDLRHISMGYKMKLYFQTNPYFTNMVIVKEFQRNRSGRLVSHSTPIRWHRGQEPQAYNRRSHDTRESFFNWFSNHSLPEADRIAEIIKNDLWVNPVRYYMRRGGYRSSRKKQHGKERAKNQYEMVIMEDAHDHYAIEDILSDISEIDEITDNETIHDIKISDFMETTDYFETTDNEVTDANENLCDSENPDHSEGYNTKITDNKGSVAANPDDNSDDPEEKNTYDSEDSNSEKADGDNTTLRDNQQVTNIQDSSDSDNGDEGSDDEDDDGNEGDNEGSDDDDDDNEGSDDDDRDIRYYKNGPEVFDKALDNRTNQNDYEEEVELISEDSVEEEEETSEEASQLSEDSYEDERIYGEERSEVNSEDSDIQEVLPVPKAWASLGKKGKIG

Target ORF information:

RefSeq Version	NM_029836.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSPY-like 2 (Tspyl2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029836.4

ORF Insert Sequence:

ATGGACCGCC CGGACGAGGG GCCTCCGGCC AAGACCCCCC GCCTGAGCAG CTCGGAACCC 
CGTCAGCGCG ACCTGCCGCC CCCGCCACCC CCACCGCTCC AGCGACTGCC CCTGCCTCCA 
CCCCAGCAGC GTCCGAGACC CCAGGAAGAA ACCGAGGCTG CACAGGTGCT GGCTGACATG 
AGGGGGGTGG GCCCCACGCT GCCCCCACCA CTGCCCTATG TCATTCTTGA GGAAGGGGGG 
ATCCGCGCGT ATTTCACCCT GAGTGCTGAG AGTCCCGGAT GGGATCACGC AATGGAGTCA 
GGGTTCGGGG AGGCCCCTTC CACAGGGATC ATGGAAACGC TCCCATCTTC AGAAATCTCT 
GGGGGAAGCC TGGCAATTGA CTTTCAGGTT GCAGAGCCCA GCAGCCTCGG AGAGAAGGCC 
CTAGAAACCT GTAGCTTAGG AGGGTGGGGG CCCCAGATGT TAGTTGGTCC GAAGAGGAAG 
GAGGAGGCTA TCATAATAGT GGAAGATGAG GATGAGGATG ACAAGGAGAG TGTGAGGAGG 
CGGCAGCGTC GGAGGAGGAG GAGAAGGAAG CAGAGGAAGG CGAAGGAGAG CCGAGAGAGG 
AGTGCCCAGA GGATGGAGAG CATACTGCAG GCCCTGGAGA GCATTCAAAT GGACCTGGAG 
GCGGTGAACA TCAAGGCAGG CAAGGCCTTC TTGCGTCTCA AGCGCAAATT CATCCAGATG 
CGAAGGCCCT TTTTGGAGCG CAGAGACCTC ATCATCCAGC ATATCCCAGG CTTCTGGGTC 
AAAGCATTCC TCAACCACCC CAGAATTTCA ATCTTGATCA ACCAACGTGA CAGAGACATT 
TTCCGCTACT TGACCAATCT TCAGGTACAG GACCTCAGAC ATATCTCCAT GGGCTACAAA 
ATGAAGCTGT ACTTCCAGAC AAACCCATAC TTTACAAACA TGGTGATCGT CAAGGAGTTC 
CAGCGCAACC GCTCAGGCCG GTTGGTGTCT CATTCTACCC CAATACGTTG GCACCGGGGC 
CAGGAACCCC AGGCCTATAA TCGCAGGAGC CATGACACCA GAGAAAGCTT CTTCAACTGG 
TTTTCCAATC ACAGCCTCCC AGAAGCTGAC AGAATTGCTG AGATCATCAA GAACGACCTG 
TGGGTTAACC CAGTACGCTA CTATATGAGA CGAGGTGGCT ACAGATCAAG CAGAAAGAAG 
CAACACGGAA AGGAAAGAGC CAAGAATCAA TATGAAATGG TGATCATGGA AGATGCTCAT 
GACCATTATG CAATTGAAGA CATTCTCAGT GACATCTCAG AAATTGATGA GATCACTGAC 
AATGAGACCA TTCACGACAT CAAGATCTCT GACTTCATGG AGACCACCGA CTACTTCGAG 
ACTACTGATA ATGAGGTGAC CGATGCCAAT GAGAACCTGT GCGACAGTGA GAATCCTGAC 
CACAGTGAAG GCTACAACAC CAAGATCACT GACAACAAAG GGAGCGTTGC CGCAAATCCA 
GATGACAACA GTGATGACCC AGAGGAAAAG AATACCTATG ACAGTGAGGA TTCCAACAGC 
GAGAAAGCTG ATGGTGATAA CACAACCCTA CGTGACAACC AGCAGGTTAC TAATATCCAG 
GACAGCAGTG ATAGTGACAA TGGAGATGAG GGCAGTGATG ATGAAGATGA TGATGGTAAT 
GAGGGGGACA ATGAGGGCAG TGATGATGAT GATGATGACA ATGAGGGCAG TGATGATGAT 
GACAGGGACA TTAGATACTA TAAGAATGGC CCTGAAGTCT TTGACAAGGC TCTGGATAAC 
CGCACCAACC AGAATGACTA TGAGGAGGAG GTAGAACTCA TCTCTGAAGA CTCAGTTGAA 
GAAGAGGAAG AGACCAGTGA GGAAGCCAGC CAGCTAAGTG AGGACAGCTA TGAAGATGAG 
CGAATCTATG GGGAGGAAAG AAGTGAGGTG AACTCCGAAG ATTCTGATAT TCAGGAAGTG 
CTGCCGGTCC CAAAAGCTTG GGCCAGCCTG GGGAAGAAAG GGAAAATCGG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSPYL2 ( NM_001358369.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001345298.1 Mus musculus TSPY-like 2 (Tspyl2), transcript variant 3, mRNA.

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CloneID	OMu56698
Clone ID Related Accession (Same CDS sequence)	NM_001358369.1 , XM_006528908.3
Accession Version	NM_001358369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Mus musculus(house mouse)
Product	testis-specific Y-encoded-like protein 2 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL731727.18 and AW046203.1. On Dec 1, 2017 this sequence version replaced XM_006528908.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.39810.1, SRR1660817.86354.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGCC CGGACGAGGG GCCTCCGGCC AAGACCCCCC GCCTGAGCAG CTCGGAACCC 
CGTCAGCGCG ACCTGCCGCC CCCGCCACCC CCACCGCTCC AGCGACTGCC CCTGCCTCCA 
CCCCAGCAGC GTCCGAGACC CCAGGAAGAA ACCGAGGCTG CACAGGTGCT GGCTGACATG 
AGGGGGGTGG GCCCCACGCT GCCCCCACCA CTGCCCTATG TCATTCTTGA GGAAGGGGGG 
ATCCGCGCGT ATTTCACCCT GAGTGCTGAG AGTCCCGGAT GGGATCACGC AATGGAGTCA 
GGGTTCGGGG AGGCCCCTTC CACAGGGATC ATGGAAACGC TCCCATCTTC AGAAATCTCT 
GGGGGAAGCC TGGCAATTGA CTTTCAGGTT GCAGAGCCCA GCAGCCTCGG AGAGAAGGCC 
CTAGAAACCT GTAGCTTAGG AGGGTGGGGG CCCCAGATGT TAGTTGGTCC GAAGAGGAAG 
GAGGAGGCTA TCATAATAGT GGAAGATGAG GATGAGGATG ACAAGGAGAG TGTGAGGAGG 
CGGCAGCGTC GGAGGAGGAG GAGAAGGAAG CAGAGGAAGG CGAAGGAGAG CCGAGAGAGG 
AGTGCCCAGA GGATGGAGAG CATACTGCAG GCCCTGGAGA GCATTCAAAT GGACCTGGAG 
GCGGTGAACA TCAAGGCAGG CAAGGCCTTC TTGCGTCTCA AGCGCAAATT CATCCAGATG 
CGAAGGCCCT TTTTGGAGCG CAGAGACCTC ATCATCCAGC ATATCCCAGG CTTCTGGGTC 
AAAGCATTCC TCAACCACCC CAGAATTTCA ATCTTGATCA ACCAACGTGA CAGAGACATT 
TTCCGCTACT TGACCAATCT TCAGGTACAG GACCTCAGAC ATATCTCCAT GGGCTACAAA 
ATGAAGCTGT ACTTCCAGAC AAACCCATAC TTTACAAACA TGGTGATCGT CAAGGAGTTC 
CAGCGCAACC GCTCAGGCCG GTTGGTGTCT CATTCTACCC CAATACGTTG GCACCGGGGC 
CAGGAACCCC AGGCCTATAA TCGCAGGAGC CATGACACCA GAGAAAGCTT CTTCAACTGG 
TTTTCCAATC ACAGCCTCCC AGAAGCTGAC AGAATTGCTG AGATCATCAA GAACGACCTG 
TGGGTTAACC CAGTACGCTA CTATATGAGA CGAGGTGGCT ACAGATCAAG CAGAAAGAAG 
CAACACGGAA AGGAAAGTAG AGCCAAGAAT CAATATGAAA TGGTGATCAT GGAAGATGCT 
CATGACCATT ATGCAATTGA AGACATTCTC AGTGACATCT CAGAAATTGA TGAGATCACT 
GACAATGAGA CCATTCACGA CATCAAGATC TCTGACTTCA TGGAGACCAC CGACTACTTC 
GAGACTACTG ATAATGAGGT GACCGATGCC AATGAGAACC TGTGCGACAG TGAGAATCCT 
GACCACAGTG AAGGCTACAA CACCAAGATC ACTGACAACA AAGGGAGCGT TGCCGCAAAT 
CCAGATGACA ACAGTGATGA CCCAGAGGAA AAGAATACCT ATGACAGTGA GGATTCCAAC 
AGCGAGAAAG CTGATGGTGA TAACACAACC CTACGTGACA ACCAGCAGGT TACTAATATC 
CAGGACAGCA GTGATAGTGA CAATGGAGAT GAGGGCAGTG ATGATGAAGA TGATGATGGT 
AATGAGGGGG ACAATGAGGG CAGTGATGAT GATGATGATG ACAATGAGGG CAGTGATGAT 
GATGACAGGG ACATTAGATA CTATAAGAAT GGCCCTGAAG TCTTTGACAA GGCTCTGGAT 
AACCGCACCA ACCAGAATGA CTATGAGGAG GAGGTAGAAC TCATCTCTGA AGACTCAGTT 
GAAGAAGAGG AAGAGACCAG TGAGGAAGCC AGCCAGCTAA GTGAGGACAG CTATGAAGAT 
GAGCGAATCT ATGGGGAGGA AAGAAGTGAG GTGAACTCCG AAGATTCTGA TATTCAGGAA 
GTGCTGCCGG TCCCAAAAGC TTGGGCCAGC CTGGGGAAGA AAGGGAAAAT CGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345298.1
CDS	160..2196
Translation	MDRPDEGPPAKTPRLSSSEPRQRDLPPPPPPPLQRLPLPPPQQRPRPQEETEAAQVLADMRGVGPTLPPPLPYVILEEGGIRAYFTLSAESPGWDHAMESGFGEAPSTGIMETLPSSEISGGSLAIDFQVAEPSSLGEKALETCSLGGWGPQMLVGPKRKEEAIIIVEDEDEDDKESVRRRQRRRRRRRKQRKAKESRERSAQRMESILQALESIQMDLEAVNIKAGKAFLRLKRKFIQMRRPFLERRDLIIQHIPGFWVKAFLNHPRISILINQRDRDIFRYLTNLQVQDLRHISMGYKMKLYFQTNPYFTNMVIVKEFQRNRSGRLVSHSTPIRWHRGQEPQAYNRRSHDTRESFFNWFSNHSLPEADRIAEIIKNDLWVNPVRYYMRRGGYRSSRKKQHGKESRAKNQYEMVIMEDAHDHYAIEDILSDISEIDEITDNETIHDIKISDFMETTDYFETTDNEVTDANENLCDSENPDHSEGYNTKITDNKGSVAANPDDNSDDPEEKNTYDSEDSNSEKADGDNTTLRDNQQVTNIQDSSDSDNGDEGSDDEDDDGNEGDNEGSDDDDDDNEGSDDDDRDIRYYKNGPEVFDKALDNRTNQNDYEEEVELISEDSVEEEEETSEEASQLSEDSYEDERIYGEERSEVNSEDSDIQEVLPVPKAWASLGKKGKIG

Target ORF information:

RefSeq Version	NM_001358369.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus TSPY-like 2 (Tspyl2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358369.1

ORF Insert Sequence:

ATGGACCGCC CGGACGAGGG GCCTCCGGCC AAGACCCCCC GCCTGAGCAG CTCGGAACCC 
CGTCAGCGCG ACCTGCCGCC CCCGCCACCC CCACCGCTCC AGCGACTGCC CCTGCCTCCA 
CCCCAGCAGC GTCCGAGACC CCAGGAAGAA ACCGAGGCTG CACAGGTGCT GGCTGACATG 
AGGGGGGTGG GCCCCACGCT GCCCCCACCA CTGCCCTATG TCATTCTTGA GGAAGGGGGG 
ATCCGCGCGT ATTTCACCCT GAGTGCTGAG AGTCCCGGAT GGGATCACGC AATGGAGTCA 
GGGTTCGGGG AGGCCCCTTC CACAGGGATC ATGGAAACGC TCCCATCTTC AGAAATCTCT 
GGGGGAAGCC TGGCAATTGA CTTTCAGGTT GCAGAGCCCA GCAGCCTCGG AGAGAAGGCC 
CTAGAAACCT GTAGCTTAGG AGGGTGGGGG CCCCAGATGT TAGTTGGTCC GAAGAGGAAG 
GAGGAGGCTA TCATAATAGT GGAAGATGAG GATGAGGATG ACAAGGAGAG TGTGAGGAGG 
CGGCAGCGTC GGAGGAGGAG GAGAAGGAAG CAGAGGAAGG CGAAGGAGAG CCGAGAGAGG 
AGTGCCCAGA GGATGGAGAG CATACTGCAG GCCCTGGAGA GCATTCAAAT GGACCTGGAG 
GCGGTGAACA TCAAGGCAGG CAAGGCCTTC TTGCGTCTCA AGCGCAAATT CATCCAGATG 
CGAAGGCCCT TTTTGGAGCG CAGAGACCTC ATCATCCAGC ATATCCCAGG CTTCTGGGTC 
AAAGCATTCC TCAACCACCC CAGAATTTCA ATCTTGATCA ACCAACGTGA CAGAGACATT 
TTCCGCTACT TGACCAATCT TCAGGTACAG GACCTCAGAC ATATCTCCAT GGGCTACAAA 
ATGAAGCTGT ACTTCCAGAC AAACCCATAC TTTACAAACA TGGTGATCGT CAAGGAGTTC 
CAGCGCAACC GCTCAGGCCG GTTGGTGTCT CATTCTACCC CAATACGTTG GCACCGGGGC 
CAGGAACCCC AGGCCTATAA TCGCAGGAGC CATGACACCA GAGAAAGCTT CTTCAACTGG 
TTTTCCAATC ACAGCCTCCC AGAAGCTGAC AGAATTGCTG AGATCATCAA GAACGACCTG 
TGGGTTAACC CAGTACGCTA CTATATGAGA CGAGGTGGCT ACAGATCAAG CAGAAAGAAG 
CAACACGGAA AGGAAAGTAG AGCCAAGAAT CAATATGAAA TGGTGATCAT GGAAGATGCT 
CATGACCATT ATGCAATTGA AGACATTCTC AGTGACATCT CAGAAATTGA TGAGATCACT 
GACAATGAGA CCATTCACGA CATCAAGATC TCTGACTTCA TGGAGACCAC CGACTACTTC 
GAGACTACTG ATAATGAGGT GACCGATGCC AATGAGAACC TGTGCGACAG TGAGAATCCT 
GACCACAGTG AAGGCTACAA CACCAAGATC ACTGACAACA AAGGGAGCGT TGCCGCAAAT 
CCAGATGACA ACAGTGATGA CCCAGAGGAA AAGAATACCT ATGACAGTGA GGATTCCAAC 
AGCGAGAAAG CTGATGGTGA TAACACAACC CTACGTGACA ACCAGCAGGT TACTAATATC 
CAGGACAGCA GTGATAGTGA CAATGGAGAT GAGGGCAGTG ATGATGAAGA TGATGATGGT 
AATGAGGGGG ACAATGAGGG CAGTGATGAT GATGATGATG ACAATGAGGG CAGTGATGAT 
GATGACAGGG ACATTAGATA CTATAAGAAT GGCCCTGAAG TCTTTGACAA GGCTCTGGAT 
AACCGCACCA ACCAGAATGA CTATGAGGAG GAGGTAGAAC TCATCTCTGA AGACTCAGTT 
GAAGAAGAGG AAGAGACCAG TGAGGAAGCC AGCCAGCTAA GTGAGGACAG CTATGAAGAT 
GAGCGAATCT ATGGGGAGGA AAGAAGTGAG GTGAACTCCG AAGATTCTGA TATTCAGGAA 
GTGCTGCCGG TCCCAAAAGC TTGGGCCAGC CTGGGGAAGA AAGGGAAAAT CGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TSPYL2 ( XM_006528908.3 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006528971.1 Mus musculus TSPY-like 2 (Tspyl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56698
Clone ID Related Accession (Same CDS sequence)	NM_001358369.1 , XM_006528908.3
Accession Version	XM_006528908.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	testis-specific Y-encoded-like protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528908.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGCC CGGACGAGGG GCCTCCGGCC AAGACCCCCC GCCTGAGCAG CTCGGAACCC 
CGTCAGCGCG ACCTGCCGCC CCCGCCACCC CCACCGCTCC AGCGACTGCC CCTGCCTCCA 
CCCCAGCAGC GTCCGAGACC CCAGGAAGAA ACCGAGGCTG CACAGGTGCT GGCTGACATG 
AGGGGGGTGG GCCCCACGCT GCCCCCACCA CTGCCCTATG TCATTCTTGA GGAAGGGGGG 
ATCCGCGCGT ATTTCACCCT GAGTGCTGAG AGTCCCGGAT GGGATCACGC AATGGAGTCA 
GGGTTCGGGG AGGCCCCTTC CACAGGGATC ATGGAAACGC TCCCATCTTC AGAAATCTCT 
GGGGGAAGCC TGGCAATTGA CTTTCAGGTT GCAGAGCCCA GCAGCCTCGG AGAGAAGGCC 
CTAGAAACCT GTAGCTTAGG AGGGTGGGGG CCCCAGATGT TAGTTGGTCC GAAGAGGAAG 
GAGGAGGCTA TCATAATAGT GGAAGATGAG GATGAGGATG ACAAGGAGAG TGTGAGGAGG 
CGGCAGCGTC GGAGGAGGAG GAGAAGGAAG CAGAGGAAGG CGAAGGAGAG CCGAGAGAGG 
AGTGCCCAGA GGATGGAGAG CATACTGCAG GCCCTGGAGA GCATTCAAAT GGACCTGGAG 
GCGGTGAACA TCAAGGCAGG CAAGGCCTTC TTGCGTCTCA AGCGCAAATT CATCCAGATG 
CGAAGGCCCT TTTTGGAGCG CAGAGACCTC ATCATCCAGC ATATCCCAGG CTTCTGGGTC 
AAAGCATTCC TCAACCACCC CAGAATTTCA ATCTTGATCA ACCAACGTGA CAGAGACATT 
TTCCGCTACT TGACCAATCT TCAGGTACAG GACCTCAGAC ATATCTCCAT GGGCTACAAA 
ATGAAGCTGT ACTTCCAGAC AAACCCATAC TTTACAAACA TGGTGATCGT CAAGGAGTTC 
CAGCGCAACC GCTCAGGCCG GTTGGTGTCT CATTCTACCC CAATACGTTG GCACCGGGGC 
CAGGAACCCC AGGCCTATAA TCGCAGGAGC CATGACACCA GAGAAAGCTT CTTCAACTGG 
TTTTCCAATC ACAGCCTCCC AGAAGCTGAC AGAATTGCTG AGATCATCAA GAACGACCTG 
TGGGTTAACC CAGTACGCTA CTATATGAGA CGAGGTGGCT ACAGATCAAG CAGAAAGAAG 
CAACACGGAA AGGAAAGTAG AGCCAAGAAT CAATATGAAA TGGTGATCAT GGAAGATGCT 
CATGACCATT ATGCAATTGA AGACATTCTC AGTGACATCT CAGAAATTGA TGAGATCACT 
GACAATGAGA CCATTCACGA CATCAAGATC TCTGACTTCA TGGAGACCAC CGACTACTTC 
GAGACTACTG ATAATGAGGT GACCGATGCC AATGAGAACC TGTGCGACAG TGAGAATCCT 
GACCACAGTG AAGGCTACAA CACCAAGATC ACTGACAACA AAGGGAGCGT TGCCGCAAAT 
CCAGATGACA ACAGTGATGA CCCAGAGGAA AAGAATACCT ATGACAGTGA GGATTCCAAC 
AGCGAGAAAG CTGATGGTGA TAACACAACC CTACGTGACA ACCAGCAGGT TACTAATATC 
CAGGACAGCA GTGATAGTGA CAATGGAGAT GAGGGCAGTG ATGATGAAGA TGATGATGGT 
AATGAGGGGG ACAATGAGGG CAGTGATGAT GATGATGATG ACAATGAGGG CAGTGATGAT 
GATGACAGGG ACATTAGATA CTATAAGAAT GGCCCTGAAG TCTTTGACAA GGCTCTGGAT 
AACCGCACCA ACCAGAATGA CTATGAGGAG GAGGTAGAAC TCATCTCTGA AGACTCAGTT 
GAAGAAGAGG AAGAGACCAG TGAGGAAGCC AGCCAGCTAA GTGAGGACAG CTATGAAGAT 
GAGCGAATCT ATGGGGAGGA AAGAAGTGAG GTGAACTCCG AAGATTCTGA TATTCAGGAA 
GTGCTGCCGG TCCCAAAAGC TTGGGCCAGC CTGGGGAAGA AAGGGAAAAT CGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528971.1
CDS	179..2215
Translation	MDRPDEGPPAKTPRLSSSEPRQRDLPPPPPPPLQRLPLPPPQQRPRPQEETEAAQVLADMRGVGPTLPPPLPYVILEEGGIRAYFTLSAESPGWDHAMESGFGEAPSTGIMETLPSSEISGGSLAIDFQVAEPSSLGEKALETCSLGGWGPQMLVGPKRKEEAIIIVEDEDEDDKESVRRRQRRRRRRRKQRKAKESRERSAQRMESILQALESIQMDLEAVNIKAGKAFLRLKRKFIQMRRPFLERRDLIIQHIPGFWVKAFLNHPRISILINQRDRDIFRYLTNLQVQDLRHISMGYKMKLYFQTNPYFTNMVIVKEFQRNRSGRLVSHSTPIRWHRGQEPQAYNRRSHDTRESFFNWFSNHSLPEADRIAEIIKNDLWVNPVRYYMRRGGYRSSRKKQHGKESRAKNQYEMVIMEDAHDHYAIEDILSDISEIDEITDNETIHDIKISDFMETTDYFETTDNEVTDANENLCDSENPDHSEGYNTKITDNKGSVAANPDDNSDDPEEKNTYDSEDSNSEKADGDNTTLRDNQQVTNIQDSSDSDNGDEGSDDEDDDGNEGDNEGSDDDDDDNEGSDDDDRDIRYYKNGPEVFDKALDNRTNQNDYEEEVELISEDSVEEEEETSEEASQLSEDSYEDERIYGEERSEVNSEDSDIQEVLPVPKAWASLGKKGKIG

Target ORF information:

RefSeq Version	XM_006528908.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus TSPY-like 2 (Tspyl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528908.3

ORF Insert Sequence:

ATGGACCGCC CGGACGAGGG GCCTCCGGCC AAGACCCCCC GCCTGAGCAG CTCGGAACCC 
CGTCAGCGCG ACCTGCCGCC CCCGCCACCC CCACCGCTCC AGCGACTGCC CCTGCCTCCA 
CCCCAGCAGC GTCCGAGACC CCAGGAAGAA ACCGAGGCTG CACAGGTGCT GGCTGACATG 
AGGGGGGTGG GCCCCACGCT GCCCCCACCA CTGCCCTATG TCATTCTTGA GGAAGGGGGG 
ATCCGCGCGT ATTTCACCCT GAGTGCTGAG AGTCCCGGAT GGGATCACGC AATGGAGTCA 
GGGTTCGGGG AGGCCCCTTC CACAGGGATC ATGGAAACGC TCCCATCTTC AGAAATCTCT 
GGGGGAAGCC TGGCAATTGA CTTTCAGGTT GCAGAGCCCA GCAGCCTCGG AGAGAAGGCC 
CTAGAAACCT GTAGCTTAGG AGGGTGGGGG CCCCAGATGT TAGTTGGTCC GAAGAGGAAG 
GAGGAGGCTA TCATAATAGT GGAAGATGAG GATGAGGATG ACAAGGAGAG TGTGAGGAGG 
CGGCAGCGTC GGAGGAGGAG GAGAAGGAAG CAGAGGAAGG CGAAGGAGAG CCGAGAGAGG 
AGTGCCCAGA GGATGGAGAG CATACTGCAG GCCCTGGAGA GCATTCAAAT GGACCTGGAG 
GCGGTGAACA TCAAGGCAGG CAAGGCCTTC TTGCGTCTCA AGCGCAAATT CATCCAGATG 
CGAAGGCCCT TTTTGGAGCG CAGAGACCTC ATCATCCAGC ATATCCCAGG CTTCTGGGTC 
AAAGCATTCC TCAACCACCC CAGAATTTCA ATCTTGATCA ACCAACGTGA CAGAGACATT 
TTCCGCTACT TGACCAATCT TCAGGTACAG GACCTCAGAC ATATCTCCAT GGGCTACAAA 
ATGAAGCTGT ACTTCCAGAC AAACCCATAC TTTACAAACA TGGTGATCGT CAAGGAGTTC 
CAGCGCAACC GCTCAGGCCG GTTGGTGTCT CATTCTACCC CAATACGTTG GCACCGGGGC 
CAGGAACCCC AGGCCTATAA TCGCAGGAGC CATGACACCA GAGAAAGCTT CTTCAACTGG 
TTTTCCAATC ACAGCCTCCC AGAAGCTGAC AGAATTGCTG AGATCATCAA GAACGACCTG 
TGGGTTAACC CAGTACGCTA CTATATGAGA CGAGGTGGCT ACAGATCAAG CAGAAAGAAG 
CAACACGGAA AGGAAAGTAG AGCCAAGAAT CAATATGAAA TGGTGATCAT GGAAGATGCT 
CATGACCATT ATGCAATTGA AGACATTCTC AGTGACATCT CAGAAATTGA TGAGATCACT 
GACAATGAGA CCATTCACGA CATCAAGATC TCTGACTTCA TGGAGACCAC CGACTACTTC 
GAGACTACTG ATAATGAGGT GACCGATGCC AATGAGAACC TGTGCGACAG TGAGAATCCT 
GACCACAGTG AAGGCTACAA CACCAAGATC ACTGACAACA AAGGGAGCGT TGCCGCAAAT 
CCAGATGACA ACAGTGATGA CCCAGAGGAA AAGAATACCT ATGACAGTGA GGATTCCAAC 
AGCGAGAAAG CTGATGGTGA TAACACAACC CTACGTGACA ACCAGCAGGT TACTAATATC 
CAGGACAGCA GTGATAGTGA CAATGGAGAT GAGGGCAGTG ATGATGAAGA TGATGATGGT 
AATGAGGGGG ACAATGAGGG CAGTGATGAT GATGATGATG ACAATGAGGG CAGTGATGAT 
GATGACAGGG ACATTAGATA CTATAAGAAT GGCCCTGAAG TCTTTGACAA GGCTCTGGAT 
AACCGCACCA ACCAGAATGA CTATGAGGAG GAGGTAGAAC TCATCTCTGA AGACTCAGTT 
GAAGAAGAGG AAGAGACCAG TGAGGAAGCC AGCCAGCTAA GTGAGGACAG CTATGAAGAT 
GAGCGAATCT ATGGGGAGGA AAGAAGTGAG GTGAACTCCG AAGATTCTGA TATTCAGGAA 
GTGCTGCCGG TCCCAAAAGC TTGGGCCAGC CTGGGGAAGA AAGGGAAAAT CGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diabetes Reduces Severity of Aortic Aneurysms Depending on the Presence of Cell Division Autoantigen 1 (CDA1).
Diabetes(2018 Jan)
Li J,Huynh P,Dai A,Wu T,Tu Y,Chow B,Kiriazis H,Du XJ,Bach LA,Wilkinson-Berka JL,Biros E,Walker P,Nataatmadja M,West M,Golledge J,Allen TJ,Cooper ME,Chai Z

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The ATPase hCINAP regulates 18S rRNA processing and is essential for embryogenesis and tumour growth.
Nature communications712310(2016 Aug)
Bai D,Zhang J,Li T,Hang R,Liu Y,Tian Y,Huang D,Qu L,Cao X,Ji J,Zheng X

Tspyl2 Loss-of-Function Causes Neurodevelopmental Brain and Behavior Abnormalities in Mice.
Behavior genetics46(4)529-37(2016 Jul)
Li Q,Chan SY,Wong KK,Wei R,Leung YO,Ding AY,Hui TC,Cheung C,Chua SE,Sham PC,Wu EX,McAlonan GM

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TSPYL2 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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