Myo1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myo1a
Entrez Gene ID	432516
Full Name	myosin IA
Synonyms	BBM-I,Myhl
General protein information	Preferred Names myosin IA Names unconventional myosin-Ia BBMI MIHC brush border myosin 1 brush border myosin I myosin I heavy chain myosin, heavy polypeptide-like (110kD) myosin-Ia
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 D3\|10 74.62 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006513859.3	XP_006513922.1	unconventional myosin-Ia isoform X1
NM_001081219.2	NP_001074688.1	unconventional myosin-Ia
XM_006513860.3	XP_006513923.1	unconventional myosin-Ia isoform X1
XM_006513859.4	XP_006513922.1	unconventional myosin-Ia isoform X1
XM_006513860.4	XP_006513923.1	unconventional myosin-Ia isoform X1

Pathways from BioSystems

Homologies

	MYO1A	XP_001115546.1
Canis lupus familiaris (dog)	MYO1A	XP_531642.2
Rattus norvegicus (Norway rat)	Myo1a	NP_062197.1
Pan troglodytes (chimpanzee)	MYO1A	XP_003313620.1
Bos taurus (cattle)	MYO1A	NP_776820.1
Mus musculus (house mouse)	Myo1a	NP_001074688.1
Gallus gallus (chicken)	MYO1A	NP_990494.1
Xenopus tropicalis (tropical clawed frog)	myo1a	NP_001008063.1
Homo sapiens (human)	MYO1A	NP_001242970.1

Gene Ontology
Bibliography

Related articles in PubMed

Restoration of cytoskeletal and membrane tethering defects but not defects in membrane trafficking in the intestinal brush border of mice lacking both myosin Ia and myosin VI.
Hegan PS, Kravtsov DV, Caputo C, Egan ME, Ameen NA, Mooseker MS
Cytoskeleton (Hoboken, N.J.)72(9)455-76(2015 Sep)

Myosin Ia is required for CFTR brush border membrane trafficking and ion transport in the mouse small intestine.
Kravtsov DV, Caputo C, Collaco A, Hoekstra N, Egan ME, Mooseker MS, Ameen NA
Traffic (Copenhagen, Denmark)13(8)1072-82(2012 Aug)

Brush border myosin Ia has tumor suppressor activity in the intestine.
Mazzolini R, Dopeso H, Mateo-Lozano S, Chang W, Rodrigues P, Bazzocco S, Alazzouzi H, Landolfi S, Hern?ndez-Losa J, Andretta E, Alhopuro P, Esp?n E, Armengol M, Tabernero J, Ram?n y Cajal S, Kloor M, Gebert J, Mariadason JM, Schwartz S Jr, Aaltonen LA, Mooseker MS, Arango D
Proceedings of the National Academy of Sciences of the United States of America109(5)1530-5(2012 Jan)

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Revenu C, Ubelmann F, Hurbain I, El-Marjou F, Dingli F, Loew D, Delacour D, Gilet J, Brot-Laroche E, Rivero F, Louvard D, Robine S
Molecular biology of the cell23(2)324-36(2012 Jan)

The transcriptional repressor Blimp1/Prdm1 regulates postnatal reprogramming of intestinal enterocytes.
Harper J, Mould A, Andrews RM, Bikoff EK, Robertson EJ
Proceedings of the National Academy of Sciences of the United States of America108(26)10585-90(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Myo1a and Myo6 play essential roles in response to intestinal mucosal injury
Title: Restoration of cytoskeletal and membrane tethering defects but not defects in membrane trafficking in the intestinal brush border of mice lacking both myosin Ia and myosin VI.

The data indicates that Myo1a is an important regulator of CFTR traffic and anion transport in the brush border membrane of villus enterocytes.
Title: Myosin Ia is required for CFTR brush border membrane trafficking and ion transport in the mouse small intestine.

Data suggest that Myo1d may be the primary compensating class I myosin in the Myo1a KO model; they also suggest that dynamics govern the localization and function of different yet closely related myosins that target common actin structures.
Title: Differential localization and dynamics of class I myosins in the enterocyte microvillus.

These studies show that class I myosins directly control the mechanical properties of the cell membrane; they also position these motor proteins as master regulators of cellular events involving membrane deformation.
Title: Control of cell membrane tension by myosin-I.

critical multifunctional component of the enterocyte, required for maintaining the normal composition and highly ordered structure of the brush border
Title: Myosin-1a is critical for normal brush border structure and composition.

The following Myo1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myo1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21253	XM_006513860.4 Latest version!	Mus musculus myosin IA (Myo1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu21253	XM_006513859.4 Latest version!	Mus musculus myosin IA (Myo1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu21253	NM_001081219.2 Latest version!	Mus musculus myosin IA (Myo1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu21253	XM_006513859.3	Mus musculus myosin IA (Myo1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu21253	XM_006513860.3	Mus musculus myosin IA (Myo1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myo1a ( XM_006513859.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513922.1 Mus musculus myosin IA (Myo1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21253
Clone ID Related Accession (Same CDS sequence)	XM_006513859.3 , NM_001081219.2 , XM_006513860.3 , XM_006513859.4 , XM_006513860.4
Accession Version	XM_006513859.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ia isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513859.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513922.1
CDS	284..3415
Translation	MPLLEGPVGVEDLILLEPLDEESLIKNLQLRYENKEIYTYIGNVVISMNPYEQLPIYGPEFIAKYRDYTFYELKPHIYALANVAYQSLKDRDRDQCILITGESGAGKTEASKLVMSYVAAVCGKGEQVNSVKEQLLQSNPVLEAFGNAKTIRNNNSSRFGKYMDIEFDFKGSPLGGVITNYLLEKSRVVKQLKGERNFHIFYQLLAGADAQLLKALKLEEDTSVYGYLNGEVSKVNGMDDASNFRAVQHAMSVIGFSEEEIRQVLEVTALVLKLGNVKLTDEFQANGIPASGICDGKGIQEIGEMMGLNSTELERALCSRTMETGKEKVVTVLNVTQAQYARDALAKNIYSRLFDWIVKRINESIKVGTGEKKKVMGVLDIYGFEILEDNSFEQFVINYCNERLQQVFIELTLKEEQEEYKREGIPWTKVEYFDNGIICNLIEHSQRGILAMLDEECLRPGVVSDSTFLAKLNQLFSKHSHYESKVSQNAQRQYDRTMGLSCFRISHYAGKVTYNVTGFIDKNNDLLFRDLSQTMWKAQHPLLKSLFPEGNPKEASLKRPPTAGTQFKNSVAVLMKNLYSKNPNYIRCIKPNDQQQKGRFTSEMVMVQARYLGLLENVRVRRAGYAFRQGYKPFLERYRLLSRSTWPRWNGDDREGVEKVLGSLTLSSEELAYGKTKIFIRSPKTLFYLEEQRRLRLQQLATLIQKVYRGWRCRTHYQQMRKSQILISAWFRGNKQKKHYGKIRSSVLLIQAFVRGWRARKNYRKYFRSGAALTLANFIYQSMAQKFLLNLKKNLPSTKVLDNTWPAAPYRCFNTANQELQRLFYQWKCKKFRDQLSPKQVQTLREKLCASELFKGKKASYPQSVPIPFRGDYIGLQGNPKLQRLKGREEGPVLVADTVKKVNRGNGKTSARILLLTKGHVILTDAKKSQAQIVIGLEDVAGVSVSSLQDGLFSLHLSEMSSAVSKGDILLVSDHVVELLTKMYQAVLDATQRQLSVTVTEKFSVRFKEGSVAVKVIQGPEGGGNRKLICKKKGSNAMEVTVR

Target ORF information:

RefSeq Version	XM_006513859.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IA (Myo1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513859.4

ORF Insert Sequence:

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1a ( XM_006513860.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513923.1 Mus musculus myosin IA (Myo1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu21253
Clone ID Related Accession (Same CDS sequence)	XM_006513859.3 , NM_001081219.2 , XM_006513860.3 , XM_006513859.4 , XM_006513860.4
Accession Version	XM_006513860.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ia isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513860.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513923.1
CDS	298..3429
Translation	MPLLEGPVGVEDLILLEPLDEESLIKNLQLRYENKEIYTYIGNVVISMNPYEQLPIYGPEFIAKYRDYTFYELKPHIYALANVAYQSLKDRDRDQCILITGESGAGKTEASKLVMSYVAAVCGKGEQVNSVKEQLLQSNPVLEAFGNAKTIRNNNSSRFGKYMDIEFDFKGSPLGGVITNYLLEKSRVVKQLKGERNFHIFYQLLAGADAQLLKALKLEEDTSVYGYLNGEVSKVNGMDDASNFRAVQHAMSVIGFSEEEIRQVLEVTALVLKLGNVKLTDEFQANGIPASGICDGKGIQEIGEMMGLNSTELERALCSRTMETGKEKVVTVLNVTQAQYARDALAKNIYSRLFDWIVKRINESIKVGTGEKKKVMGVLDIYGFEILEDNSFEQFVINYCNERLQQVFIELTLKEEQEEYKREGIPWTKVEYFDNGIICNLIEHSQRGILAMLDEECLRPGVVSDSTFLAKLNQLFSKHSHYESKVSQNAQRQYDRTMGLSCFRISHYAGKVTYNVTGFIDKNNDLLFRDLSQTMWKAQHPLLKSLFPEGNPKEASLKRPPTAGTQFKNSVAVLMKNLYSKNPNYIRCIKPNDQQQKGRFTSEMVMVQARYLGLLENVRVRRAGYAFRQGYKPFLERYRLLSRSTWPRWNGDDREGVEKVLGSLTLSSEELAYGKTKIFIRSPKTLFYLEEQRRLRLQQLATLIQKVYRGWRCRTHYQQMRKSQILISAWFRGNKQKKHYGKIRSSVLLIQAFVRGWRARKNYRKYFRSGAALTLANFIYQSMAQKFLLNLKKNLPSTKVLDNTWPAAPYRCFNTANQELQRLFYQWKCKKFRDQLSPKQVQTLREKLCASELFKGKKASYPQSVPIPFRGDYIGLQGNPKLQRLKGREEGPVLVADTVKKVNRGNGKTSARILLLTKGHVILTDAKKSQAQIVIGLEDVAGVSVSSLQDGLFSLHLSEMSSAVSKGDILLVSDHVVELLTKMYQAVLDATQRQLSVTVTEKFSVRFKEGSVAVKVIQGPEGGGNRKLICKKKGSNAMEVTVR

Target ORF information:

RefSeq Version	XM_006513860.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IA (Myo1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513860.4

ORF Insert Sequence:

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1a ( NM_001081219.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074688.1 Mus musculus myosin IA (Myo1a), mRNA.

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CloneID	OMu21253
Clone ID Related Accession (Same CDS sequence)	XM_006513859.3 , NM_001081219.2 , XM_006513860.3 , XM_006513859.4 , XM_006513860.4
Accession Version	NM_001081219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ia
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF009960.1, AC160970.7, AK008176.1 and CF929471.1. On or before Feb 15, 2015 this sequence version replaced XM_011251968.1, XM_006542881.1, NM_001081219.1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC147605.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074688.1
CDS	1..3132
Translation	MPLLEGPVGVEDLILLEPLDEESLIKNLQLRYENKEIYTYIGNVVISMNPYEQLPIYGPEFIAKYRDYTFYELKPHIYALANVAYQSLKDRDRDQCILITGESGAGKTEASKLVMSYVAAVCGKGEQVNSVKEQLLQSNPVLEAFGNAKTIRNNNSSRFGKYMDIEFDFKGSPLGGVITNYLLEKSRVVKQLKGERNFHIFYQLLAGADAQLLKALKLEEDTSVYGYLNGEVSKVNGMDDASNFRAVQHAMSVIGFSEEEIRQVLEVTALVLKLGNVKLTDEFQANGIPASGICDGKGIQEIGEMMGLNSTELERALCSRTMETGKEKVVTVLNVTQAQYARDALAKNIYSRLFDWIVKRINESIKVGTGEKKKVMGVLDIYGFEILEDNSFEQFVINYCNERLQQVFIELTLKEEQEEYKREGIPWTKVEYFDNGIICNLIEHSQRGILAMLDEECLRPGVVSDSTFLAKLNQLFSKHSHYESKVSQNAQRQYDRTMGLSCFRISHYAGKVTYNVTGFIDKNNDLLFRDLSQTMWKAQHPLLKSLFPEGNPKEASLKRPPTAGTQFKNSVAVLMKNLYSKNPNYIRCIKPNDQQQKGRFTSEMVMVQARYLGLLENVRVRRAGYAFRQGYKPFLERYRLLSRSTWPRWNGDDREGVEKVLGSLTLSSEELAYGKTKIFIRSPKTLFYLEEQRRLRLQQLATLIQKVYRGWRCRTHYQQMRKSQILISAWFRGNKQKKHYGKIRSSVLLIQAFVRGWRARKNYRKYFRSGAALTLANFIYQSMAQKFLLNLKKNLPSTKVLDNTWPAAPYRCFNTANQELQRLFYQWKCKKFRDQLSPKQVQTLREKLCASELFKGKKASYPQSVPIPFRGDYIGLQGNPKLQRLKGREEGPVLVADTVKKVNRGNGKTSARILLLTKGHVILTDAKKSQAQIVIGLEDVAGVSVSSLQDGLFSLHLSEMSSAVSKGDILLVSDHVVELLTKMYQAVLDATQRQLSVTVTEKFSVRFKEGSVAVKVIQGPEGGGNRKLICKKKGSNAMEVTVR

Target ORF information:

RefSeq Version	NM_001081219.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IA (Myo1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081219.2

ORF Insert Sequence:

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1a ( XM_006513859.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513922.1 Mus musculus myosin IA (Myo1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu21253
Clone ID Related Accession (Same CDS sequence)	XM_006513859.3 , NM_001081219.2 , XM_006513860.3 , XM_006513859.4 , XM_006513860.4
Accession Version	XM_006513859.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ia isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513859.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513922.1
CDS	284..3415
Translation	MPLLEGPVGVEDLILLEPLDEESLIKNLQLRYENKEIYTYIGNVVISMNPYEQLPIYGPEFIAKYRDYTFYELKPHIYALANVAYQSLKDRDRDQCILITGESGAGKTEASKLVMSYVAAVCGKGEQVNSVKEQLLQSNPVLEAFGNAKTIRNNNSSRFGKYMDIEFDFKGSPLGGVITNYLLEKSRVVKQLKGERNFHIFYQLLAGADAQLLKALKLEEDTSVYGYLNGEVSKVNGMDDASNFRAVQHAMSVIGFSEEEIRQVLEVTALVLKLGNVKLTDEFQANGIPASGICDGKGIQEIGEMMGLNSTELERALCSRTMETGKEKVVTVLNVTQAQYARDALAKNIYSRLFDWIVKRINESIKVGTGEKKKVMGVLDIYGFEILEDNSFEQFVINYCNERLQQVFIELTLKEEQEEYKREGIPWTKVEYFDNGIICNLIEHSQRGILAMLDEECLRPGVVSDSTFLAKLNQLFSKHSHYESKVSQNAQRQYDRTMGLSCFRISHYAGKVTYNVTGFIDKNNDLLFRDLSQTMWKAQHPLLKSLFPEGNPKEASLKRPPTAGTQFKNSVAVLMKNLYSKNPNYIRCIKPNDQQQKGRFTSEMVMVQARYLGLLENVRVRRAGYAFRQGYKPFLERYRLLSRSTWPRWNGDDREGVEKVLGSLTLSSEELAYGKTKIFIRSPKTLFYLEEQRRLRLQQLATLIQKVYRGWRCRTHYQQMRKSQILISAWFRGNKQKKHYGKIRSSVLLIQAFVRGWRARKNYRKYFRSGAALTLANFIYQSMAQKFLLNLKKNLPSTKVLDNTWPAAPYRCFNTANQELQRLFYQWKCKKFRDQLSPKQVQTLREKLCASELFKGKKASYPQSVPIPFRGDYIGLQGNPKLQRLKGREEGPVLVADTVKKVNRGNGKTSARILLLTKGHVILTDAKKSQAQIVIGLEDVAGVSVSSLQDGLFSLHLSEMSSAVSKGDILLVSDHVVELLTKMYQAVLDATQRQLSVTVTEKFSVRFKEGSVAVKVIQGPEGGGNRKLICKKKGSNAMEVTVR

Target ORF information:

RefSeq Version	XM_006513859.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IA (Myo1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513859.3

ORF Insert Sequence:

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myo1a ( XM_006513860.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513923.1 Mus musculus myosin IA (Myo1a), transcript variant X2, mRNA.

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CloneID	OMu21253
Clone ID Related Accession (Same CDS sequence)	XM_006513859.3 , NM_001081219.2 , XM_006513860.3 , XM_006513859.4 , XM_006513860.4
Accession Version	XM_006513860.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	unconventional myosin-Ia isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513923.1
CDS	285..3416
Translation	MPLLEGPVGVEDLILLEPLDEESLIKNLQLRYENKEIYTYIGNVVISMNPYEQLPIYGPEFIAKYRDYTFYELKPHIYALANVAYQSLKDRDRDQCILITGESGAGKTEASKLVMSYVAAVCGKGEQVNSVKEQLLQSNPVLEAFGNAKTIRNNNSSRFGKYMDIEFDFKGSPLGGVITNYLLEKSRVVKQLKGERNFHIFYQLLAGADAQLLKALKLEEDTSVYGYLNGEVSKVNGMDDASNFRAVQHAMSVIGFSEEEIRQVLEVTALVLKLGNVKLTDEFQANGIPASGICDGKGIQEIGEMMGLNSTELERALCSRTMETGKEKVVTVLNVTQAQYARDALAKNIYSRLFDWIVKRINESIKVGTGEKKKVMGVLDIYGFEILEDNSFEQFVINYCNERLQQVFIELTLKEEQEEYKREGIPWTKVEYFDNGIICNLIEHSQRGILAMLDEECLRPGVVSDSTFLAKLNQLFSKHSHYESKVSQNAQRQYDRTMGLSCFRISHYAGKVTYNVTGFIDKNNDLLFRDLSQTMWKAQHPLLKSLFPEGNPKEASLKRPPTAGTQFKNSVAVLMKNLYSKNPNYIRCIKPNDQQQKGRFTSEMVMVQARYLGLLENVRVRRAGYAFRQGYKPFLERYRLLSRSTWPRWNGDDREGVEKVLGSLTLSSEELAYGKTKIFIRSPKTLFYLEEQRRLRLQQLATLIQKVYRGWRCRTHYQQMRKSQILISAWFRGNKQKKHYGKIRSSVLLIQAFVRGWRARKNYRKYFRSGAALTLANFIYQSMAQKFLLNLKKNLPSTKVLDNTWPAAPYRCFNTANQELQRLFYQWKCKKFRDQLSPKQVQTLREKLCASELFKGKKASYPQSVPIPFRGDYIGLQGNPKLQRLKGREEGPVLVADTVKKVNRGNGKTSARILLLTKGHVILTDAKKSQAQIVIGLEDVAGVSVSSLQDGLFSLHLSEMSSAVSKGDILLVSDHVVELLTKMYQAVLDATQRQLSVTVTEKFSVRFKEGSVAVKVIQGPEGGGNRKLICKKKGSNAMEVTVR

Target ORF information:

RefSeq Version	XM_006513860.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus myosin IA (Myo1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513860.3

ORF Insert Sequence:

ATGCCTCTCC TGGAAGGTCC CGTGGGGGTG GAAGACCTCA TCCTCCTGGA GCCCCTAGAT 
GAGGAGTCTC TGATCAAGAA CCTCCAACTC CGTTATGAAA ACAAAGAGAT TTATACATAC 
ATTGGGAATG TGGTGATCTC AATGAACCCC TACGAGCAGC TTCCCATCTA TGGACCAGAG 
TTCATTGCCA AATACCGGGA CTATACTTTC TATGAGCTGA AGCCTCACAT CTATGCTTTG 
GCAAATGTGG CCTACCAGTC ACTGAAGGAT CGGGACCGGG ACCAGTGTAT CCTCATCACA 
GGCGAGAGTG GAGCTGGCAA GACTGAGGCG AGTAAGCTAG TGATGTCCTA CGTGGCTGCT 
GTCTGTGGGA AAGGAGAGCA GGTGAACTCT GTGAAGGAGC AGCTGCTTCA GTCAAACCCA 
GTGCTGGAAG CATTTGGCAA TGCCAAGACC ATCCGCAATA ACAACTCCTC TCGATTTGGA 
AAGTACATGG ACATCGAGTT TGACTTCAAA GGGTCCCCCC TGGGCGGTGT CATCACCAAC 
TATCTGCTTG AGAAGTCCCG AGTGGTGAAG CAACTCAAAG GGGAGAGGAA TTTCCATATC 
TTCTATCAAC TGCTGGCTGG AGCAGACGCA CAGCTGCTGA AGGCCCTAAA GCTTGAGGAG 
GACACGAGTG TATACGGCTA CCTGAATGGG GAGGTGTCCA AGGTGAACGG TATGGACGAC 
GCCTCCAACT TCAGGGCTGT GCAGCATGCG ATGTCAGTGA TTGGCTTCTC AGAGGAGGAA 
ATTAGACAAG TGCTGGAGGT GACAGCCCTG GTGCTGAAGC TGGGGAACGT GAAGCTGACA 
GACGAGTTCC AGGCCAATGG GATCCCAGCC AGTGGCATCT GTGATGGGAA AGGTATTCAG 
GAGATTGGGG AGATGATGGG CTTGAACTCT ACGGAACTAG AGAGAGCGCT GTGCTCAAGA 
ACTATGGAGA CAGGCAAAGA GAAGGTGGTC ACGGTGCTGA ATGTCACACA GGCTCAATAT 
GCTCGGGATG CCCTGGCAAA GAATATTTAC AGCCGCCTCT TCGACTGGAT AGTGAAACGC 
ATCAACGAGA GCATCAAGGT GGGCACTGGG GAGAAGAAGA AGGTTATGGG GGTCCTGGAT 
ATCTACGGCT TTGAGATATT GGAGGATAAC AGCTTTGAGC AGTTCGTGAT CAACTACTGC 
AATGAGAGGC TACAGCAGGT GTTCATAGAA CTGACCCTGA AGGAAGAACA GGAGGAATAC 
AAGAGAGAAG GCATACCGTG GACAAAGGTG GAATACTTTG ACAACGGCAT CATCTGTAAC 
CTCATTGAAC ATAGTCAGCG AGGTATCCTG GCCATGCTGG ACGAGGAGTG CCTGAGACCC 
GGGGTGGTTA GTGACTCCAC CTTCTTAGCG AAGCTGAACC AGCTCTTCTC TAAGCACAGT 
CACTACGAGA GTAAAGTCTC CCAGAATGCC CAGCGCCAGT ACGACCGCAC CATGGGCCTC 
AGCTGCTTCC GCATCAGCCA CTACGCGGGC AAGGTGACCT ACAACGTGAC CGGCTTCATC 
GACAAGAATA ACGACCTACT CTTCCGAGAC CTATCCCAGA CAATGTGGAA GGCCCAGCAT 
CCCCTCCTGA AGTCCTTGTT TCCAGAGGGG AACCCCAAGG AAGCGTCTCT CAAACGACCC 
CCGACTGCCG GGACCCAGTT TAAGAATTCG GTGGCTGTAC TCATGAAGAA CCTGTATTCT 
AAGAACCCCA ACTACATCAG GTGCATAAAG CCCAATGACC AGCAGCAGAA AGGCAGGTTC 
ACCTCAGAGA TGGTTATGGT CCAGGCGCGA TACCTGGGGC TGCTGGAGAA TGTTCGGGTG 
CGCAGGGCTG GCTATGCCTT CCGCCAGGGC TATAAGCCTT TCCTGGAAAG GTACCGATTG 
CTGAGCCGGA GCACCTGGCC TCGCTGGAAT GGGGATGATC GGGAAGGTGT GGAGAAGGTA 
CTCGGGTCTC TGACTTTGTC CTCAGAGGAG CTGGCCTACG GGAAGACAAA GATCTTCATC 
AGAAGCCCCA AAACTCTTTT CTACCTGGAA GAACAAAGGC GCCTGAGGCT TCAGCAGCTG 
GCCACCCTCA TACAGAAGGT CTACCGCGGC TGGCGCTGTC GGACCCACTA CCAGCAGATG 
CGGAAGAGCC AAATCCTCAT CTCGGCTTGG TTTCGGGGCA ACAAGCAAAA GAAGCACTAT 
GGGAAGATAC GGTCGTCAGT GCTGCTGATC CAGGCTTTCG TGAGGGGCTG GAGGGCCCGC 
AAGAACTATC GAAAATACTT CCGGTCAGGT GCTGCCCTCA CCTTGGCAAA TTTTATCTAC 
CAGAGCATGG CACAGAAATT CCTCTTGAAT CTGAAGAAGA ATTTGCCATC CACCAAAGTG 
TTGGACAACA CATGGCCAGC CGCCCCTTAC AGGTGCTTCA ACACAGCCAA TCAGGAGCTG 
CAGCGCCTCT TCTACCAGTG GAAGTGCAAG AAGTTCCGAG ATCAGCTGTC CCCGAAGCAG 
GTGCAGACAC TGCGGGAAAA GCTCTGTGCC AGTGAACTGT TTAAGGGCAA GAAGGCTTCC 
TACCCCCAGA GTGTGCCTAT CCCGTTCCGT GGTGATTACA TCGGGCTGCA AGGGAACCCC 
AAGCTGCAGA GACTGAAGGG CAGGGAGGAG GGGCCTGTCC TGGTGGCAGA CACCGTGAAG 
AAGGTCAACC GTGGCAATGG CAAGACATCG GCGCGGATTC TCCTCCTGAC CAAGGGGCAC 
GTGATTCTCA CGGATGCGAA GAAGTCCCAG GCCCAGATTG TCATAGGACT GGAGGATGTG 
GCTGGGGTGT CAGTCAGCAG CCTCCAGGAC GGGCTCTTTA GCTTGCATCT GAGTGAGATG 
TCATCGGCAG TCTCCAAGGG GGACATCCTG CTAGTGAGCG ACCATGTGGT TGAACTGCTG 
ACAAAAATGT ACCAAGCAGT GTTGGATGCC ACACAGAGGC AGCTCTCTGT CACTGTGACT 
GAGAAGTTCT CAGTGAGGTT CAAGGAGGGC AGTGTGGCTG TCAAGGTTAT CCAGGGCCCC 
GAGGGGGGTG GGAACAGAAA ACTGATTTGC AAGAAGAAGG GGAGTAATGC CATGGAGGTG 
ACTGTGCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Restoration of cytoskeletal and membrane tethering defects but not defects in membrane trafficking in the intestinal brush border of mice lacking both myosin Ia and myosin VI.
Cytoskeleton (Hoboken, N.J.)72(9)455-76(2015 Sep)
Hegan PS,Kravtsov DV,Caputo C,Egan ME,Ameen NA,Mooseker MS

Myosin Ia is required for CFTR brush border membrane trafficking and ion transport in the mouse small intestine.
Traffic (Copenhagen, Denmark)13(8)1072-82(2012 Aug)
Kravtsov DV,Caputo C,Collaco A,Hoekstra N,Egan ME,Mooseker MS,Ameen NA

Brush border myosin Ia has tumor suppressor activity in the intestine.
Proceedings of the National Academy of Sciences of the United States of America109(5)1530-5(2012 Jan)
Mazzolini R,Dopeso H,Mateo-Lozano S,Chang W,Rodrigues P,Bazzocco S,Alazzouzi H,Landolfi S,Hern?ndez-Losa J,Andretta E,Alhopuro P,Esp?n E,Armengol M,Tabernero J,Ram?n y Cajal S,Kloor M,Gebert J,Mariadason JM,Schwartz S Jr,Aaltonen LA,Mooseker MS,Arango D

A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Molecular biology of the cell23(2)324-36(2012 Jan)
Revenu C,Ubelmann F,Hurbain I,El-Marjou F,Dingli F,Loew D,Delacour D,Gilet J,Brot-Laroche E,Rivero F,Louvard D,Robine S

The transcriptional repressor Blimp1/Prdm1 regulates postnatal reprogramming of intestinal enterocytes.
Proceedings of the National Academy of Sciences of the United States of America108(26)10585-90(2011 Jun)
Harper J,Mould A,Andrews RM,Bikoff EK,Robertson EJ

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Myo1a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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