Mamld1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mamld1
Entrez Gene ID	333639
Full Name	mastermind-like domain containing 1
Synonyms	G630014P10Rik
General protein information	Preferred Names mastermind-like domain containing 1 Names mastermind-like domain-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X A7.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017318531.2	XP_017174020.1	mastermind-like domain-containing protein 1 isoform X2
XM_011247622.2	XP_011245924.1	mastermind-like domain-containing protein 1 isoform X1
XM_017318531.1	XP_017174020.1	mastermind-like domain-containing protein 1 isoform X2
NM_001256048.1	NP_001242977.1	mastermind-like domain-containing protein 1 isoform 2
NM_001081354.2	NP_001074823.2	mastermind-like domain-containing protein 1 isoform 1
XM_011247622.3	XP_011245924.1	mastermind-like domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAMLD1	XP_521302.3
Mus musculus (house mouse)	Mamld1	NP_001074823.2

Gene Ontology
Bibliography

Related articles in PubMed

Knockout of Murine Mamld1 Impairs Testicular Growth and Daily Sperm Production but Permits Normal Postnatal Androgen Production and Fertility.
Miyado M, Yoshida K, Miyado K, Katsumi M, Saito K, Nakamura S, Ogata T, Fukami M
International journal of molecular sciences18(6)(2017 Jun)

Parturition failure in mice lacking Mamld1.
Miyado M, Miyado K, Katsumi M, Saito K, Nakamura A, Shihara D, Ogata T, Fukami M
Scientific reports514705(2015 Oct)

The candidate splicing factor Sfswap regulates growth and patterning of inner ear sensory organs.
Moayedi Y, Basch ML, Pacheco NL, Gao SS, Wang R, Harrison W, Xiao N, Oghalai JS, Overbeek PA, Mardon G, Groves AK
PLoS genetics10(1)e1004055(2014 Jan)

Mamld1 deficiency significantly reduces mRNA expression levels of multiple genes expressed in mouse fetal Leydig cells but permits normal genital and reproductive development.
Miyado M, Nakamura M, Miyado K, Morohashi K, Sano S, Nagata E, Fukami M, Ogata T
Endocrinology153(12)6033-40(2012 Dec)

Mamld1 knockdown reduces testosterone production and Cyp17a1 expression in mouse Leydig tumor cells.
Nakamura M, Fukami M, Sugawa F, Miyado M, Nonomura K, Ogata T
PloS one6(4)e19123(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MAMLD1 likely participates in functional luteolysis by regulating Stat5b and other genes, independent of the PGF2alpha signaling pathway.
Title: Parturition failure in mice lacking Mamld1.

Mamld1 deficiency significantly reduces mRNA expression levels of multiple genes expressed in mouse fetal Leydig cells but permits normal genital and reproductive development
Title: Mamld1 deficiency significantly reduces mRNA expression levels of multiple genes expressed in mouse fetal Leydig cells but permits normal genital and reproductive development.

Mamld1 enhances Cyp17a1 expression primarily in Leydig cells and permit to produce a sufficient amount of testosterone for male sex development, independently of the Hes3-related non-canonical Notch signaling
Title: Mamld1 knockdown reduces testosterone production and Cyp17a1 expression in mouse Leydig tumor cells.

CXorf6 transactivates the Hes3 promoter, augments testosterone production
Title: Mastermind-like domain-containing 1 (MAMLD1 or CXorf6) transactivates the Hes3 promoter, augments testosterone production, and contains the SF1 target sequence.

The following Mamld1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mamld1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02193	XM_011247622.3 Latest version!	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu83142	XM_017318531.2 Latest version!	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu02193	NM_001081354.2 Latest version!	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu05856	NM_001256048.1 Latest version!	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu83142	XM_017318531.1	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu02193	XM_011247622.2	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mamld1 ( XM_011247622.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245924.1 Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02193
Clone ID Related Accession (Same CDS sequence)	XM_011247622.2 , NM_001081354.2 , XM_011247622.3
Accession Version	XM_011247622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mastermind-like domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG GGCTGTTGCC ATCGCTGTGC GTGGACTTCT 
ACGGCTCTCT GGCTGGAGCA CCAGCATCAG CAGTGGAACT CTCTCACTAG CACTCATGGA 
CATGTGCCAC CATCTAACCT TACTCATGTA GACAAAGCCT GCAAGCTTGG GGAAGCCAGG 
CCTCCCCATG TCAGCCTGGG GCGACAGCCC CCATCCCGCC AGGCCCTGGG GAGTGAGTCC 
TTCCTGCCCG GCAGCTCCTT TGCTCATGAG CTGGCCCGAG TCACATCCAC CTCCTCGTAC 
AACACCTCAG AGGCAGCCCC CTGGGGCGGC TGGGATCCGA AGGCCTGGAG GCAGGTGCCT 
GCTCCACTAC TGCCTAGCTG CGATGCTGCA GCCAGAGAAG CAGAGATCAG GTCCTATGGC 
AATGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245924.1
CDS	378..2789
Translation	MDDWKSRLVIENMLPHFNMVGNRQETRKLQESGTSKRRQEGENFHFTGMADGSYPNKIKRPCLEDVTLSMGPGAHPTSLSTEMQMPTLNMNPTSADLGVAGQSLLLENNPLDSNVVNSSAMGSPFGVPSTADTGLKGHAVPYYEKNNSMPAVDQELQDLLEELTKIQEPSSNDLDLEKILGSKPEEPLVLHNPQAPLGPPAKLPVQMPHMESLGSSKEFASSCSQVAGTSLPIMPSSTGMSYSIPSSSKQIVSSSSSTAQAQVKNQVQNMLPVTMPPLSVPQWHHAHQLKALAASKQGSATKQGSNRNWSSLPPPGLSPPYLPVPSPHPPPPQPPPPPFSPQNFTASCMSSSSLSGSAVQSSPNALLSSMAPSSNASLGPTLPYVPAKLPGLPLNQQPQFSPQSSILANLVSSSVKSPQGHLISALPTSTPGPSPPYRPENLSSPGLPQQSFTPQYSLIRSLTPTSNLLSQQQQQQQQQQQQQQQQQQQQQQQQHQANSIFKPMTSSQQPKTLSMIMQQGLSSSSPEAPEPFTFSNTKPLSHFVSEPSPQKMASMSTHSRQSSLLHYLPQATPAHAPSATASSTATATLQLQHHHQQHHHQQHHHQQQHHQQQHHQQHHHQQQQHQQQQHQQQQQQQPDQSSFLLQQIMQQPQRFQRMMASDSMPALPGQGCCHRCAWTSTALWLEHQHQQWNSLTSTHGHVPPSNLTHVDKACKLGEARPPHVSLGRQPPSRQALGSESFLPGSSFAHELARVTSTSSYNTSEAAPWGGWDPKAWRQVPAPLLPSCDAAAREAEIRSYGNDP

Target ORF information:

RefSeq Version	XM_011247622.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247622.3

ORF Insert Sequence:

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG GGCTGTTGCC ATCGCTGTGC GTGGACTTCT 
ACGGCTCTCT GGCTGGAGCA CCAGCATCAG CAGTGGAACT CTCTCACTAG CACTCATGGA 
CATGTGCCAC CATCTAACCT TACTCATGTA GACAAAGCCT GCAAGCTTGG GGAAGCCAGG 
CCTCCCCATG TCAGCCTGGG GCGACAGCCC CCATCCCGCC AGGCCCTGGG GAGTGAGTCC 
TTCCTGCCCG GCAGCTCCTT TGCTCATGAG CTGGCCCGAG TCACATCCAC CTCCTCGTAC 
AACACCTCAG AGGCAGCCCC CTGGGGCGGC TGGGATCCGA AGGCCTGGAG GCAGGTGCCT 
GCTCCACTAC TGCCTAGCTG CGATGCTGCA GCCAGAGAAG CAGAGATCAG GTCCTATGGC 
AATGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mamld1 ( XM_017318531.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174020.1 Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu83142
Clone ID Related Accession (Same CDS sequence)	XM_017318531.2 , XM_017318531.1
Accession Version	XM_017318531.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mastermind-like domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318531.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG CCAGAGAAGC AGAGATCAGG TCCTATGGCA 
ATGACCCCTA AACAGCAGAA TGTGTGTATC GCTCAGCAGA TGACTCAATT TCAAGTACAA 
GAACAAGTCA TCTCCAAGTG TAATCAAACC AAGAAGAGTC CCCCATCCAA CCAGCAAAAG 
ATGCCACCCA AAAATGAGCT CCTTCAGGAC AATCAGATTC CAAGAATATT ACCACCCACA 
AGTCACATGG GTCATCAACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174020.1
CDS	378..2621
Translation	MDDWKSRLVIENMLPHFNMVGNRQETRKLQESGTSKRRQEGENFHFTGMADGSYPNKIKRPCLEDVTLSMGPGAHPTSLSTEMQMPTLNMNPTSADLGVAGQSLLLENNPLDSNVVNSSAMGSPFGVPSTADTGLKGHAVPYYEKNNSMPAVDQELQDLLEELTKIQEPSSNDLDLEKILGSKPEEPLVLHNPQAPLGPPAKLPVQMPHMESLGSSKEFASSCSQVAGTSLPIMPSSTGMSYSIPSSSKQIVSSSSSTAQAQVKNQVQNMLPVTMPPLSVPQWHHAHQLKALAASKQGSATKQGSNRNWSSLPPPGLSPPYLPVPSPHPPPPQPPPPPFSPQNFTASCMSSSSLSGSAVQSSPNALLSSMAPSSNASLGPTLPYVPAKLPGLPLNQQPQFSPQSSILANLVSSSVKSPQGHLISALPTSTPGPSPPYRPENLSSPGLPQQSFTPQYSLIRSLTPTSNLLSQQQQQQQQQQQQQQQQQQQQQQQQHQANSIFKPMTSSQQPKTLSMIMQQGLSSSSPEAPEPFTFSNTKPLSHFVSEPSPQKMASMSTHSRQSSLLHYLPQATPAHAPSATASSTATATLQLQHHHQQHHHQQHHHQQQHHQQQHHQQHHHQQQQHQQQQHQQQQQQQPDQSSFLLQQIMQQPQRFQRMMASDSMPALPGQPEKQRSGPMAMTPKQQNVCIAQQMTQFQVQEQVISKCNQTKKSPPSNQQKMPPKNELLQDNQIPRILPPTSHMGHQL

Target ORF information:

RefSeq Version	XM_017318531.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318531.2

ORF Insert Sequence:

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG CCAGAGAAGC AGAGATCAGG TCCTATGGCA 
ATGACCCCTA AACAGCAGAA TGTGTGTATC GCTCAGCAGA TGACTCAATT TCAAGTACAA 
GAACAAGTCA TCTCCAAGTG TAATCAAACC AAGAAGAGTC CCCCATCCAA CCAGCAAAAG 
ATGCCACCCA AAAATGAGCT CCTTCAGGAC AATCAGATTC CAAGAATATT ACCACCCACA 
AGTCACATGG GTCATCAACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mamld1 ( NM_001081354.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074823.2 Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant 1, mRNA.

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CloneID	OMu02193
Clone ID Related Accession (Same CDS sequence)	XM_011247622.2 , NM_001081354.2 , XM_011247622.3
Accession Version	NM_001081354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	mastermind-like domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY733469.1 and AL731843.14. On Dec 24, 2011 this sequence version replaced NM_001081354.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: BC157923.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG GGCTGTTGCC ATCGCTGTGC GTGGACTTCT 
ACGGCTCTCT GGCTGGAGCA CCAGCATCAG CAGTGGAACT CTCTCACTAG CACTCATGGA 
CATGTGCCAC CATCTAACCT TACTCATGTA GACAAAGCCT GCAAGCTTGG GGAAGCCAGG 
CCTCCCCATG TCAGCCTGGG GCGACAGCCC CCATCCCGCC AGGCCCTGGG GAGTGAGTCC 
TTCCTGCCCG GCAGCTCCTT TGCTCATGAG CTGGCCCGAG TCACATCCAC CTCCTCGTAC 
AACACCTCAG AGGCAGCCCC CTGGGGCGGC TGGGATCCGA AGGCCTGGAG GCAGGTGCCT 
GCTCCACTAC TGCCTAGCTG CGATGCTGCA GCCAGAGAAG CAGAGATCAG GTCCTATGGC 
AATGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074823.2
CDS	158..2569
Translation	MDDWKSRLVIENMLPHFNMVGNRQETRKLQESGTSKRRQEGENFHFTGMADGSYPNKIKRPCLEDVTLSMGPGAHPTSLSTEMQMPTLNMNPTSADLGVAGQSLLLENNPLDSNVVNSSAMGSPFGVPSTADTGLKGHAVPYYEKNNSMPAVDQELQDLLEELTKIQEPSSNDLDLEKILGSKPEEPLVLHNPQAPLGPPAKLPVQMPHMESLGSSKEFASSCSQVAGTSLPIMPSSTGMSYSIPSSSKQIVSSSSSTAQAQVKNQVQNMLPVTMPPLSVPQWHHAHQLKALAASKQGSATKQGSNRNWSSLPPPGLSPPYLPVPSPHPPPPQPPPPPFSPQNFTASCMSSSSLSGSAVQSSPNALLSSMAPSSNASLGPTLPYVPAKLPGLPLNQQPQFSPQSSILANLVSSSVKSPQGHLISALPTSTPGPSPPYRPENLSSPGLPQQSFTPQYSLIRSLTPTSNLLSQQQQQQQQQQQQQQQQQQQQQQQQHQANSIFKPMTSSQQPKTLSMIMQQGLSSSSPEAPEPFTFSNTKPLSHFVSEPSPQKMASMSTHSRQSSLLHYLPQATPAHAPSATASSTATATLQLQHHHQQHHHQQHHHQQQHHQQQHHQQHHHQQQQHQQQQHQQQQQQQPDQSSFLLQQIMQQPQRFQRMMASDSMPALPGQGCCHRCAWTSTALWLEHQHQQWNSLTSTHGHVPPSNLTHVDKACKLGEARPPHVSLGRQPPSRQALGSESFLPGSSFAHELARVTSTSSYNTSEAAPWGGWDPKAWRQVPAPLLPSCDAAAREAEIRSYGNDP

Target ORF information:

RefSeq Version	NM_001081354.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081354.2

ORF Insert Sequence:

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG GGCTGTTGCC ATCGCTGTGC GTGGACTTCT 
ACGGCTCTCT GGCTGGAGCA CCAGCATCAG CAGTGGAACT CTCTCACTAG CACTCATGGA 
CATGTGCCAC CATCTAACCT TACTCATGTA GACAAAGCCT GCAAGCTTGG GGAAGCCAGG 
CCTCCCCATG TCAGCCTGGG GCGACAGCCC CCATCCCGCC AGGCCCTGGG GAGTGAGTCC 
TTCCTGCCCG GCAGCTCCTT TGCTCATGAG CTGGCCCGAG TCACATCCAC CTCCTCGTAC 
AACACCTCAG AGGCAGCCCC CTGGGGCGGC TGGGATCCGA AGGCCTGGAG GCAGGTGCCT 
GCTCCACTAC TGCCTAGCTG CGATGCTGCA GCCAGAGAAG CAGAGATCAG GTCCTATGGC 
AATGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mamld1 ( NM_001256048.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001242977.1 Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant 2, mRNA.

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CloneID	OMu05856
Clone ID Related Accession (Same CDS sequence)	NM_001256048.1
Accession Version	NM_001256048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	mastermind-like domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY733469.1 and AL731843.14. Transcript Variant: This variant (2) lacks an in-frame exon, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## CDS exon combination :: BC157989.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG ACTCATGTAG ACAAAGCCTG CAAGCTTGGG 
GAAGCCAGGC CTCCCCATGT CAGCCTGGGG CGACAGCCCC CATCCCGCCA GGCCCTGGGG 
AGTGAGTCCT TCCTGCCCGG CAGCTCCTTT GCTCATGAGC TGGCCCGAGT CACATCCACC 
TCCTCGTACA ACACCTCAGA GGCAGCCCCC TGGGGCGGCT GGGATCCGAA GGCCTGGAGG 
CAGGTGCCTG CTCCACTACT GCCTAGCTGC GATGCTGCAG CCAGAGAAGC AGAGATCAGG 
TCCTATGGCA ATGACCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001242977.1
CDS	158..2458
Translation	MDDWKSRLVIENMLPHFNMVGNRQETRKLQESGTSKRRQEGENFHFTGMADGSYPNKIKRPCLEDVTLSMGPGAHPTSLSTEMQMPTLNMNPTSADLGVAGQSLLLENNPLDSNVVNSSAMGSPFGVPSTADTGLKGHAVPYYEKNNSMPAVDQELQDLLEELTKIQEPSSNDLDLEKILGSKPEEPLVLHNPQAPLGPPAKLPVQMPHMESLGSSKEFASSCSQVAGTSLPIMPSSTGMSYSIPSSSKQIVSSSSSTAQAQVKNQVQNMLPVTMPPLSVPQWHHAHQLKALAASKQGSATKQGSNRNWSSLPPPGLSPPYLPVPSPHPPPPQPPPPPFSPQNFTASCMSSSSLSGSAVQSSPNALLSSMAPSSNASLGPTLPYVPAKLPGLPLNQQPQFSPQSSILANLVSSSVKSPQGHLISALPTSTPGPSPPYRPENLSSPGLPQQSFTPQYSLIRSLTPTSNLLSQQQQQQQQQQQQQQQQQQQQQQQQHQANSIFKPMTSSQQPKTLSMIMQQGLSSSSPEAPEPFTFSNTKPLSHFVSEPSPQKMASMSTHSRQSSLLHYLPQATPAHAPSATASSTATATLQLQHHHQQHHHQQHHHQQQHHQQQHHQQHHHQQQQHQQQQHQQQQQQQPDQSSFLLQQIMQQPQRFQRMMASDSMPALPGQTHVDKACKLGEARPPHVSLGRQPPSRQALGSESFLPGSSFAHELARVTSTSSYNTSEAAPWGGWDPKAWRQVPAPLLPSCDAAAREAEIRSYGNDP

Target ORF information:

RefSeq Version	NM_001256048.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256048.1

ORF Insert Sequence:

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG ACTCATGTAG ACAAAGCCTG CAAGCTTGGG 
GAAGCCAGGC CTCCCCATGT CAGCCTGGGG CGACAGCCCC CATCCCGCCA GGCCCTGGGG 
AGTGAGTCCT TCCTGCCCGG CAGCTCCTTT GCTCATGAGC TGGCCCGAGT CACATCCACC 
TCCTCGTACA ACACCTCAGA GGCAGCCCCC TGGGGCGGCT GGGATCCGAA GGCCTGGAGG 
CAGGTGCCTG CTCCACTACT GCCTAGCTGC GATGCTGCAG CCAGAGAAGC AGAGATCAGG 
TCCTATGGCA ATGACCCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mamld1 ( XM_011247622.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245924.1 Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02193
Clone ID Related Accession (Same CDS sequence)	XM_011247622.2 , NM_001081354.2 , XM_011247622.3
Accession Version	XM_011247622.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mastermind-like domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG GGCTGTTGCC ATCGCTGTGC GTGGACTTCT 
ACGGCTCTCT GGCTGGAGCA CCAGCATCAG CAGTGGAACT CTCTCACTAG CACTCATGGA 
CATGTGCCAC CATCTAACCT TACTCATGTA GACAAAGCCT GCAAGCTTGG GGAAGCCAGG 
CCTCCCCATG TCAGCCTGGG GCGACAGCCC CCATCCCGCC AGGCCCTGGG GAGTGAGTCC 
TTCCTGCCCG GCAGCTCCTT TGCTCATGAG CTGGCCCGAG TCACATCCAC CTCCTCGTAC 
AACACCTCAG AGGCAGCCCC CTGGGGCGGC TGGGATCCGA AGGCCTGGAG GCAGGTGCCT 
GCTCCACTAC TGCCTAGCTG CGATGCTGCA GCCAGAGAAG CAGAGATCAG GTCCTATGGC 
AATGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245924.1
CDS	366..2777
Translation	MDDWKSRLVIENMLPHFNMVGNRQETRKLQESGTSKRRQEGENFHFTGMADGSYPNKIKRPCLEDVTLSMGPGAHPTSLSTEMQMPTLNMNPTSADLGVAGQSLLLENNPLDSNVVNSSAMGSPFGVPSTADTGLKGHAVPYYEKNNSMPAVDQELQDLLEELTKIQEPSSNDLDLEKILGSKPEEPLVLHNPQAPLGPPAKLPVQMPHMESLGSSKEFASSCSQVAGTSLPIMPSSTGMSYSIPSSSKQIVSSSSSTAQAQVKNQVQNMLPVTMPPLSVPQWHHAHQLKALAASKQGSATKQGSNRNWSSLPPPGLSPPYLPVPSPHPPPPQPPPPPFSPQNFTASCMSSSSLSGSAVQSSPNALLSSMAPSSNASLGPTLPYVPAKLPGLPLNQQPQFSPQSSILANLVSSSVKSPQGHLISALPTSTPGPSPPYRPENLSSPGLPQQSFTPQYSLIRSLTPTSNLLSQQQQQQQQQQQQQQQQQQQQQQQQHQANSIFKPMTSSQQPKTLSMIMQQGLSSSSPEAPEPFTFSNTKPLSHFVSEPSPQKMASMSTHSRQSSLLHYLPQATPAHAPSATASSTATATLQLQHHHQQHHHQQHHHQQQHHQQQHHQQHHHQQQQHQQQQHQQQQQQQPDQSSFLLQQIMQQPQRFQRMMASDSMPALPGQGCCHRCAWTSTALWLEHQHQQWNSLTSTHGHVPPSNLTHVDKACKLGEARPPHVSLGRQPPSRQALGSESFLPGSSFAHELARVTSTSSYNTSEAAPWGGWDPKAWRQVPAPLLPSCDAAAREAEIRSYGNDP

Target ORF information:

RefSeq Version	XM_011247622.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247622.2

ORF Insert Sequence:

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG GGCTGTTGCC ATCGCTGTGC GTGGACTTCT 
ACGGCTCTCT GGCTGGAGCA CCAGCATCAG CAGTGGAACT CTCTCACTAG CACTCATGGA 
CATGTGCCAC CATCTAACCT TACTCATGTA GACAAAGCCT GCAAGCTTGG GGAAGCCAGG 
CCTCCCCATG TCAGCCTGGG GCGACAGCCC CCATCCCGCC AGGCCCTGGG GAGTGAGTCC 
TTCCTGCCCG GCAGCTCCTT TGCTCATGAG CTGGCCCGAG TCACATCCAC CTCCTCGTAC 
AACACCTCAG AGGCAGCCCC CTGGGGCGGC TGGGATCCGA AGGCCTGGAG GCAGGTGCCT 
GCTCCACTAC TGCCTAGCTG CGATGCTGCA GCCAGAGAAG CAGAGATCAG GTCCTATGGC 
AATGACCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mamld1 ( XM_017318531.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174020.1 Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X2, mRNA.

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CloneID	OMu83142
Clone ID Related Accession (Same CDS sequence)	XM_017318531.2 , XM_017318531.1
Accession Version	XM_017318531.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mastermind-like domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG CCAGAGAAGC AGAGATCAGG TCCTATGGCA 
ATGACCCCTA AACAGCAGAA TGTGTGTATC GCTCAGCAGA TGACTCAATT TCAAGTACAA 
GAACAAGTCA TCTCCAAGTG TAATCAAACC AAGAAGAGTC CCCCATCCAA CCAGCAAAAG 
ATGCCACCCA AAAATGAGCT CCTTCAGGAC AATCAGATTC CAAGAATATT ACCACCCACA 
AGTCACATGG GTCATCAACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174020.1
CDS	374..2617
Translation	MDDWKSRLVIENMLPHFNMVGNRQETRKLQESGTSKRRQEGENFHFTGMADGSYPNKIKRPCLEDVTLSMGPGAHPTSLSTEMQMPTLNMNPTSADLGVAGQSLLLENNPLDSNVVNSSAMGSPFGVPSTADTGLKGHAVPYYEKNNSMPAVDQELQDLLEELTKIQEPSSNDLDLEKILGSKPEEPLVLHNPQAPLGPPAKLPVQMPHMESLGSSKEFASSCSQVAGTSLPIMPSSTGMSYSIPSSSKQIVSSSSSTAQAQVKNQVQNMLPVTMPPLSVPQWHHAHQLKALAASKQGSATKQGSNRNWSSLPPPGLSPPYLPVPSPHPPPPQPPPPPFSPQNFTASCMSSSSLSGSAVQSSPNALLSSMAPSSNASLGPTLPYVPAKLPGLPLNQQPQFSPQSSILANLVSSSVKSPQGHLISALPTSTPGPSPPYRPENLSSPGLPQQSFTPQYSLIRSLTPTSNLLSQQQQQQQQQQQQQQQQQQQQQQQQHQANSIFKPMTSSQQPKTLSMIMQQGLSSSSPEAPEPFTFSNTKPLSHFVSEPSPQKMASMSTHSRQSSLLHYLPQATPAHAPSATASSTATATLQLQHHHQQHHHQQHHHQQQHHQQQHHQQHHHQQQQHQQQQHQQQQQQQPDQSSFLLQQIMQQPQRFQRMMASDSMPALPGQPEKQRSGPMAMTPKQQNVCIAQQMTQFQVQEQVISKCNQTKKSPPSNQQKMPPKNELLQDNQIPRILPPTSHMGHQL

Target ORF information:

RefSeq Version	XM_017318531.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mastermind-like domain containing 1 (Mamld1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318531.1

ORF Insert Sequence:

ATGGATGACT GGAAAAGTCG GCTTGTAATC GAGAACATGC TTCCCCATTT CAACATGGTG 
GGAAATCGTC AGGAGACCAG AAAGCTCCAG GAATCGGGAA CCAGTAAGAG AAGACAGGAA 
GGAGAGAACT TCCACTTCAC AGGCATGGCT GATGGGAGCT ATCCTAATAA AATCAAGAGA 
CCTTGCCTTG AAGATGTCAC CCTGTCTATG GGCCCAGGTG CCCACCCTAC TTCTCTCTCT 
ACAGAGATGC AGATGCCCAC ATTAAACATG AATCCCACTT CTGCAGACCT GGGAGTGGCT 
GGCCAATCAT TACTCCTTGA GAACAACCCT CTGGACAGTA ATGTTGTGAA TAGCAGTGCT 
ATGGGCTCAC CATTTGGGGT GCCATCAACT GCAGACACGG GGCTGAAAGG ACATGCTGTT 
CCTTACTATG AGAAAAACAA CAGTATGCCA GCTGTAGACC AAGAGCTTCA AGATCTGCTG 
GAGGAACTAA CCAAAATTCA AGAACCTTCT TCAAATGATC TTGACCTTGA GAAGATTCTG 
GGGAGCAAGC CAGAAGAACC TCTGGTTTTA CATAACCCCC AGGCCCCTCT TGGTCCCCCT 
GCCAAGCTTC CAGTTCAGAT GCCACATATG GAAAGCCTTG GATCCAGCAA GGAGTTTGCA 
TCTAGCTGCA GTCAAGTTGC TGGCACATCA CTACCCATCA TGCCCTCCTC CACAGGGATG 
AGCTACTCCA TTCCTTCCAG CAGTAAGCAG ATAGTCTCAT CCAGTTCTTC AACAGCACAG 
GCTCAGGTCA AAAACCAGGT CCAGAACATG CTCCCTGTCA CTATGCCTCC ATTATCTGTG 
CCTCAGTGGC ATCATGCCCA CCAGCTGAAA GCACTGGCAG CCAGCAAGCA GGGATCTGCT 
ACAAAACAAG GTTCCAACCG CAATTGGTCC AGTTTGCCTC CTCCGGGTCT TTCTCCACCT 
TACCTTCCAG TGCCATCTCC TCACCCACCA CCACCACAAC CACCACCACC ACCATTCAGC 
CCACAGAACT TCACAGCATC CTGCATGTCA TCCAGTAGCT TATCTGGCAG TGCTGTGCAA 
AGCTCGCCCA ATGCCTTACT TTCCAGCATG GCCCCCAGCA GCAATGCTTC CCTTGGGCCC 
ACCTTGCCCT ATGTTCCAGC AAAGCTCCCG GGCTTGCCTC TCAATCAGCA ACCACAATTC 
AGCCCCCAGA GCTCCATTCT TGCCAATCTG GTGTCCTCTA GTGTCAAAAG CCCTCAAGGA 
CACCTGATAT CTGCTCTGCC TACCAGCACC CCAGGGCCAT CCCCACCATA CCGCCCTGAG 
AATCTCTCCA GCCCCGGCTT GCCACAGCAG TCCTTCACTC CACAATACTC TCTGATCAGG 
AGCCTCACTC CCACCAGCAA TCTGCTAAGC CAGCAGCAGC AACAACAGCA ACAGCAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGCAA CAGCAGCAGC AGCATCAGGC AAATTCCATC 
TTTAAGCCTA TGACCAGCAG CCAGCAACCT AAAACCTTGA GCATGATCAT GCAGCAGGGC 
CTATCAAGTT CCAGTCCAGA AGCACCTGAG CCCTTTACCT TCAGCAACAC CAAGCCCTTG 
TCACATTTTG TTTCTGAGCC AAGCCCCCAG AAGATGGCCT CCATGTCTAC CCACTCCAGG 
CAGTCATCTT TGCTCCACTA CCTGCCTCAG GCAACACCAG CACATGCTCC TTCTGCCACT 
GCCTCCTCCA CTGCCACTGC CACTTTGCAA CTCCAGCATC ATCATCAACA GCATCATCAT 
CAACAGCATC ATCATCAACA GCAGCATCAT CAACAACAGC ATCATCAACA GCATCATCAT 
CAACAGCAGC AGCATCAACA GCAGCAGCAT CAACAACAAC AGCAGCAGCA ACCTGATCAG 
TCTTCATTCC TTCTGCAGCA GATAATGCAG CAACCCCAGC GCTTTCAGCG CATGATGGCT 
TCTGATTCTA TGCCTGCTCT GCCTGGACAG CCAGAGAAGC AGAGATCAGG TCCTATGGCA 
ATGACCCCTA AACAGCAGAA TGTGTGTATC GCTCAGCAGA TGACTCAATT TCAAGTACAA 
GAACAAGTCA TCTCCAAGTG TAATCAAACC AAGAAGAGTC CCCCATCCAA CCAGCAAAAG 
ATGCCACCCA AAAATGAGCT CCTTCAGGAC AATCAGATTC CAAGAATATT ACCACCCACA 
AGTCACATGG GTCATCAACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knockout of Murine Mamld1 Impairs Testicular Growth and Daily Sperm Production but Permits Normal Postnatal Androgen Production and Fertility.
International journal of molecular sciences18(6)(2017 Jun)
Miyado M,Yoshida K,Miyado K,Katsumi M,Saito K,Nakamura S,Ogata T,Fukami M

Parturition failure in mice lacking Mamld1.
Scientific reports514705(2015 Oct)
Miyado M,Miyado K,Katsumi M,Saito K,Nakamura A,Shihara D,Ogata T,Fukami M

The candidate splicing factor Sfswap regulates growth and patterning of inner ear sensory organs.
PLoS genetics10(1)e1004055(2014 Jan)
Moayedi Y,Basch ML,Pacheco NL,Gao SS,Wang R,Harrison W,Xiao N,Oghalai JS,Overbeek PA,Mardon G,Groves AK

Mamld1 deficiency significantly reduces mRNA expression levels of multiple genes expressed in mouse fetal Leydig cells but permits normal genital and reproductive development.
Endocrinology153(12)6033-40(2012 Dec)
Miyado M,Nakamura M,Miyado K,Morohashi K,Sano S,Nagata E,Fukami M,Ogata T

Mamld1 knockdown reduces testosterone production and Cyp17a1 expression in mouse Leydig tumor cells.
PloS one6(4)e19123(2011 Apr)
Nakamura M,Fukami M,Sugawa F,Miyado M,Nonomura K,Ogata T

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Mamld1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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