Slc51b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc51b
Entrez Gene ID	330962
Full Name	solute carrier family 51, beta subunit
Synonyms	Ostb
General protein information	Preferred Names solute carrier family 51, beta subunit Names organic solute transporter subunit beta OST-beta Ost beta organic solute transporter beta solute carrier family 51 subunit beta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_178933.2	NP_849264.1	organic solute transporter subunit beta

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SLC51B	XP_001174342.1
Homo sapiens (human)	SLC51B	NP_849190.2
Bos taurus (cattle)	SLC51B	NP_001071335.1
Canis lupus familiaris (dog)	SLC51B	XP_853414.1
Mus musculus (house mouse)	Slc51b	NP_849264.1
Rattus norvegicus (Norway rat)	Slc51b	XP_238546.1
	SLC51B	XP_001108335.1

Gene Ontology
Bibliography

Related articles in PubMed

Global functions of extracellular, transmembrane and cytoplasmic domains of organic solute transporter ?-subunit.
Christian WV, Hinkle PM
The Biochemical journal474(12)1981-1992(2017 05)

Cholesterol 7?-hydroxylase-deficient mice are protected from high-fat/high-cholesterol diet-induced metabolic disorders.
Ferrell JM, Boehme S, Li F, Chiang JY
Journal of lipid research57(7)1144-54(2016 07)

Maternal bile acid transporter deficiency promotes neonatal demise.
Zhang Y, Li F, Wang Y, Pitre A, Fang ZZ, Frank MW, Calabrese C, Krausz KW, Neale G, Frase S, Vogel P, Rock CO, Gonzalez FJ, Schuetz JD
Nature communications68186(2015 Sep)

Diet1 functions in the FGF15/19 enterohepatic signaling axis to modulate bile acid and lipid levels.
Vergnes L, Lee JM, Chin RG, Auwerx J, Reue K
Cell metabolism17(6)916-28(2013 Jun)

?-Subunit of the Ost?-Ost? organic solute transporter is required not only for heterodimerization and trafficking but also for function.
Christian WV, Li N, Hinkle PM, Ballatori N
The Journal of biological chemistry287(25)21233-43(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that transport of bile acid taurocholate is retained when OstB is truncated to contain only the transmembrane domain with 15 additional residues on each side and co-expressed with intact OstA (organic solute transporter alpha subunit); shorter fragments of OstB are inactive.
Title: Global functions of extracellular, transmembrane and cytoplasmic domains of organic solute transporter ?-subunit.

These findings provide direct support for the hypothesis that Ostalpha-Ostbeta is a major basolateral transporter of bile acids and conjugated steroids in the intestine, kidney, and liver.
Title: Ostalpha-Ostbeta is required for bile acid and conjugated steroid disposition in the intestine, kidney, and liver.

These data indicate that Ostalpha-Ostbeta is essential for intestinal bile acid transport in mice.
Title: The organic solute transporter alpha-beta, Ostalpha-Ostbeta, is essential for intestinal bile acid transport and homeostasis.

Present as heterodimers (with Ost alpha) and/or heteromultimers; the interaction between Ostalpha and Ostbeta increases the stability of the proteins and is required for delivery of the heteromeric complex to the plasma membrane.
Title: Heterodimerization, trafficking and membrane topology of the two proteins, Ost alpha and Ost beta, that constitute the organic solute and steroid transporter.

LXRalpha transcriptionally regulate mouse organic solute transporter alpha/beta via inverted repeat-1 elements shared with farnesoid X receptor
Title: LXR alpha transactivates mouse organic solute transporter alpha and beta via IR-1 elements shared with FXR.

The following Slc51b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc51b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18003	NM_178933.2 Latest version!	Mus musculus solute carrier family 51, beta subunit (Slc51b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc51b ( NM_178933.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_849264.1 Mus musculus solute carrier family 51, beta subunit (Slc51b), mRNA.

Price & Availability↑

CloneID	OMu18003
Clone ID Related Accession (Same CDS sequence)	NM_178933.2
Accession Version	NM_178933.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Mus musculus(house mouse)
Product	organic solute transporter subunit beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY279396.1. On Jun 3, 2003 this sequence version replaced NM_178933.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY279396.1, AK002463.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGACCACA GTGCAGAGAA AGCTGCAGCC AATGCCGAGG TGCCCCAGGA ACTGCTGGAA 
GAAATGCTTT GGTATTTTCG TGCAGAAGAT GCGGCTCCTT GGAATTATTC CATCCTGGTC 
CTGGCAGTCC TGGTGGTCAT GACAAGCATG TTCCTCCTGA GAAGGAGCAT CCTGGCAAAC 
AGAAATCGAA AGAAGCAGCC ACAAGACAAA GAAACACCAG AAGACCTGCA TCTTGATGAC 
TCCATAATGA AAGAGAACAA CAGCCAGGTC TTCCTAAGAG AGACATTGAT CTCAGAGAAA 
CCAGACTTGG CCCCGGGGGA ACCTGAGTTG AAAGAGAAAG ACTCATCACT TGTCTTTCTG 
CCAGACCCAC AGGAAACAGA GAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849264.1
CDS	58..444
Translation	MDHSAEKAAANAEVPQELLEEMLWYFRAEDAAPWNYSILVLAVLVVMTSMFLLRRSILANRNRKKQPQDKETPEDLHLDDSIMKENNSQVFLRETLISEKPDLAPGEPELKEKDSSLVFLPDPQETES

Target ORF information:

RefSeq Version	NM_178933.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 51, beta subunit (Slc51b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178933.2

ORF Insert Sequence:

ATGGACCACA GTGCAGAGAA AGCTGCAGCC AATGCCGAGG TGCCCCAGGA ACTGCTGGAA 
GAAATGCTTT GGTATTTTCG TGCAGAAGAT GCGGCTCCTT GGAATTATTC CATCCTGGTC 
CTGGCAGTCC TGGTGGTCAT GACAAGCATG TTCCTCCTGA GAAGGAGCAT CCTGGCAAAC 
AGAAATCGAA AGAAGCAGCC ACAAGACAAA GAAACACCAG AAGACCTGCA TCTTGATGAC 
TCCATAATGA AAGAGAACAA CAGCCAGGTC TTCCTAAGAG AGACATTGAT CTCAGAGAAA 
CCAGACTTGG CCCCGGGGGA ACCTGAGTTG AAAGAGAAAG ACTCATCACT TGTCTTTCTG 
CCAGACCCAC AGGAAACAGA GAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Global functions of extracellular, transmembrane and cytoplasmic domains of organic solute transporter ?-subunit.
The Biochemical journal474(12)1981-1992(2017 05)
Christian WV,Hinkle PM

Cholesterol 7?-hydroxylase-deficient mice are protected from high-fat/high-cholesterol diet-induced metabolic disorders.
Journal of lipid research57(7)1144-54(2016 07)
Ferrell JM,Boehme S,Li F,Chiang JY

Maternal bile acid transporter deficiency promotes neonatal demise.
Nature communications68186(2015 Sep)
Zhang Y,Li F,Wang Y,Pitre A,Fang ZZ,Frank MW,Calabrese C,Krausz KW,Neale G,Frase S,Vogel P,Rock CO,Gonzalez FJ,Schuetz JD

Diet1 functions in the FGF15/19 enterohepatic signaling axis to modulate bile acid and lipid levels.
Cell metabolism17(6)916-28(2013 Jun)
Vergnes L,Lee JM,Chin RG,Auwerx J,Reue K

?-Subunit of the Ost?-Ost? organic solute transporter is required not only for heterodimerization and trafficking but also for function.
The Journal of biological chemistry287(25)21233-43(2012 Jun)
Christian WV,Li N,Hinkle PM,Ballatori N