Tjp3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tjp3
Entrez Gene ID	27375
Full Name	tight junction protein 3
General protein information	Preferred Names tight junction protein 3 Names tight junction protein ZO-3 zona occludens protein 3 zonula occludens protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_013769.3	NP_038797.2	tight junction protein ZO-3 isoform 2
XM_006513708.3	XP_006513771.1	tight junction protein ZO-3 isoform X1
NM_001282095.1	NP_001269024.1	tight junction protein ZO-3 isoform 2
NM_001282096.1	NP_001269025.1	tight junction protein ZO-3 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TJP3	XP_001135804.2
Mus musculus (house mouse)	Tjp3	NP_001269025.1
Rattus norvegicus (Norway rat)	Tjp3	NP_001101543.1
Xenopus tropicalis (tropical clawed frog)	tjp3	XP_002941439.2
	TJP3	XP_001117962.1
Bos taurus (cattle)	TJP3	NP_001039339.1
Homo sapiens (human)	TJP3	NP_001254490.1
Canis lupus familiaris (dog)	TJP3	NP_001003202.1
Gallus gallus (chicken)	TJP3	XP_003642888.2

Gene Ontology
Bibliography

Related articles in PubMed

Different effects of ZO-1, ZO-2 and ZO-3 silencing on kidney collecting duct principal cell proliferation and adhesion.
Qiao X, Roth I, F?raille E, Hasler U
Cell cycle (Georgetown, Tex.)13(19)3059-75(2014)

Tight function zonula occludens-3 regulates cyclin D1-dependent cell proliferation.
Capaldo CT, Koch S, Kwon M, Laur O, Parkos CA, Nusrat A
Molecular biology of the cell22(10)1677-85(2011 May)

Roles of ZO-1 and ZO-2 in establishment of the belt-like adherens and tight junctions with paracellular permselective barrier function.
Tsukita S, Katsuno T, Yamazaki Y, Umeda K, Tamura A, Tsukita S
Annals of the New York Academy of Sciences116544-52(2009 May)

Serum response factor is required for sprouting angiogenesis and vascular integrity.
Franco CA, Mericskay M, Parlakian A, Gary-Bobo G, Gao-Li J, Paulin D, Gustafsson E, Li Z
Developmental cell15(3)448-61(2008 Sep)

Identification of transcripts with enriched expression in the developing and adult pancreas.
Hoffman BG, Zavaglia B, Witzsche J, Ruiz de Algara T, Beach M, Hoodless PA, Jones SJ, Marra MA, Helgason CD
Genome biology9(6)R99(2008)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ZO-3 shows an epithelial-specific Tight junctions localization
Title: Roles of ZO-1 and ZO-2 in establishment of the belt-like adherens and tight junctions with paracellular permselective barrier function.

Early embryonic lethality of mice lacking Tjp2, but not Tjp3, reveals critical and nonredundant roles for individual zonula occludens proteins in mammalian development.
Title: Early embryonic lethality of mice lacking ZO-2, but Not ZO-3, reveals critical and nonredundant roles for individual zonula occludens proteins in mammalian development.

ZO-3 (-/-) mice were viable and fertile and their tight junctions did not appear to be affected.
Title: Normal establishment of epithelial tight junctions in mice and cultured cells lacking expression of ZO-3, a tight-junction MAGUK protein.

The following Tjp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tjp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09924	NM_001282095.1 Latest version!	Mus musculus tight junction protein 3 (Tjp3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu09924	NM_013769.3 Latest version!	Mus musculus tight junction protein 3 (Tjp3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu09924	XM_006513708.3 Latest version!	Mus musculus tight junction protein 3 (Tjp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu08435	NM_001282096.1 Latest version!	Mus musculus tight junction protein 3 (Tjp3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tjp3 ( NM_013769.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038797.2 Mus musculus tight junction protein 3 (Tjp3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu09924
Clone ID Related Accession (Same CDS sequence)	NM_013769.3 , XM_006513708.3 , NM_001282095.1
Accession Version	NM_013769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK134100.1, AK029083.1 and AV236903.1. On Aug 18, 2013 this sequence version replaced NM_013769.2. Transcript Variant: This variant (3) differs in the 5' UTR and coding region, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK134100.1, BC012518.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGC TGACCATCTG GGAACAACAC ACAGCCACAC TGTACAAGGA CCCCCGCCGC 
GGCTTCGGCA TAGCTGTCTC TGGAGGCCAT GACCGAGCTA GTGGATCCGT GGTGGTGTCT 
GATGTGGTAC CAGGAGGCCC CGCTGAGGGC CGGCTCCGGA CCGGGGACCA CATCGTCATG 
GTGAATGGTG TCTCTGTGGA GAACGTCACA TCTGCCTTTG CCATCCAGAT CCTTAAGACC 
TGCACCAAAA CAGCCAACGT TACAGTGAAG CGTCCCCGCA GGGTCCAGCT GCCTGCCACC 
AAGGCTAGCC CAGCTTCTGG CCACCAGCTC TCAGACCAGG AAGAAGCTGA CCATGGCCGG 
GGCTATGAGG GGGACTCCTC CAGTGGCTCA GGTCGATCAT GGGGTGAGCG TTCTCGCCGG 
TCCAGGGCAG GTCGCCGCGG CAGGGTAGGC AGCCATGGGC GCCGGAGTTC GGGTGGCGGA 
TCTGAAGCCA ACGGTCTGGA CCTGGTGTCT GGCTATAAGC GCTTGCCGAA GCAGGATGTG 
CTAATGAGGC CACTAAAGTC CGTGCTGGTG AAACGAAGAA ACAGCGAAGA GTTTGGGGTG 
AAGCTAGGCA GTCAGATCTT TATAAAACAC ATCACGGAAT CTGGCCTTGC TGCTCGGAAC 
CATGGGCTGC AGGAGGGAGA CCTTATCTTA CAGATCAACG GGGTGTCCAG TGCTAATCTG 
TCCCTGAGTG ACACACGAAG GCTCATTGAG AAGTCGGAGG GGGAGCTGAC GTTGCTGGTG 
CTAAGAGACA GTGGGCAGTT CCTGGTGAAC ATCCCTCCCG CAGTGAGCGA CAGCGACAGC 
TCCCTAATGG AAGACATCTC AGACCTGACC TCAGAATTGT CCCAGGCGCC CCCTTCCCAC 
GTTCCTCCGC CACCTCTGAA GGGTCAGAGG AGCCCTGAGG ACAGCCAGAC AGACTCCCCA 
GTGGAGACCC CGCAGCCCCG GAGGCGGGAA CGGTCAGTGA ATTCAAGAGC CATCGCTGAA 
CCAGAGTCTC CTGGAGAAAG CCGCTATGAC ATTTACAGGG TTCCTAGCCG GCAGAGCCTG 
GAGGACCGTG GCTACAGCCC CGACACTCGT GTCGTGAGCT TCCCCAAGGG CGCGAGTATT 
GGCTTGCGGC TGGCCGGCGG CAACGACGTG GGCATCTTCG TATCCGGGGT GCAGGCAGGC 
AGCCCGGCCG ACGGGCAAGG CATCCAGGAA GGGGATGAGA TCTTGCAGGT GAATGGTATG 
CCATTTCGGA ACCTGACTCG GGAGGAGGCC GTGCAGTTTC TGTTGGGGCT GCCTCCAGGG 
GAAGACATGG AGCTGGTAAC ACAGAGCAAA CAGGACATCT TTAAGAAGAT GGTCCAGTCT 
CGCGTGGGTG ACTCCTTCTA CATCCGCACA CACTTTGAGC TGGAGCCGAG CCCACCCTAT 
GGCCTGGGCT TCACCCGCGG TGATGTCTTC CACGTAGTGG ACACGTTGTA CCCGGGCTCC 
GGGCCAGGCC ATGGGCACAG CAGCCATGGG GGCCTCTGGC TGGCCGCCCG CATGGGCAGA 
GATCTGCGAG AGCAAGAACG CGGCGTCATC CCCAACCAGA GCAGGGCCGA GCAGCTGGCC 
AGTCTGGAGG CTGCGCAGCG GGCCGCGGGC GTGGGGCCAG GCGCCTCAGT GGGTTCCAAC 
CCACGGGCGG AGTTTTGGCG ACTCCGAGGT CTCCGCCGAG GGACCAAGAA GGCGTCGACC 
CAGCGGAGCC GCGAGGACCT ATCAGCGCTG ACCAGGCAGG GACACTACCC GCCCTATGAG 
CGTGTGGTGC TCAGAGAAGC CAGTTTTAAG CGCCCCGTGG TGATCCTAGG ACCAGTGGCA 
GACATTGCCA TGAAGAAACT GACCACAGAG ATGCCAGAAG AGTTTGAGAT TGCAGAAAGC 
ATGTCCCGGA CTGACAGCCC ATCCAAGATC ATCAAACTGG ACACAGTGCG GGTGATCGCA 
GAGAGGGATA AGCACGCACT CCTGGATGTC ACCCCCTCTG CCATCGAGCG CCTCAACTAC 
GTGCAGTACT ACCCCATCGT CATCTTTTGT GCCCCAGAGA GCCGGCCGGC CCTCAAGGCC 
CTCCGGGAGT GGCTGGCCCC GGCCTCTCGC CGCAGCAGCC GCCGCCTGTA TGCGCAGGCC 
CAGAAACTGC AGAAACACAG TGGCCACCTC TTCACCGCCA CCATCCCCCT GCATGGCACT 
AGTGACTCCT GGTACCAGGA AGTCAAGGCT GTAATACAGC AACAGCAGGC GCGACCGATC 
TGGACCGCGG AGGACCAGCT GAACAGCTCA TCCGAGGACC TTGACCTGCC GGGCCATGGT 
CTGGCCGCCA GCTCTGGAGA CCTCAGCTGT GACAGTCGGA CCAACAGCGA CTATGAGGAC 
ACCGACGGCG AGGGCGCCTA TACGGACAGA GAGGGCGGAC CCCAGGATGT CGATGAGGAG 
GTCGCACCCA CAGCCCTGGC CCGGTCCTCA GAGCCCGTAT GGGTAGATGA TCACCAGGGG 
CTGATGGGTC ATGGGACAAC TATCACAGAC AAATGGGAGA CACAGGCTGA CAGCCACTAC 
ACCCAGGACC AGCGGCGCCA GGACAGCATG CGGACCTACA AGCACGAGGC CCTGAGGAAG 
AAGTTCACAA GAGCCCGGGA TGTGGAATCC TCTGATGACG AGGGCTATGA CTGGGGCCCA 
GCCACCGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038797.2
CDS	230..2944
Translation	MEELTIWEQHTATLYKDPRRGFGIAVSGGHDRASGSVVVSDVVPGGPAEGRLRTGDHIVMVNGVSVENVTSAFAIQILKTCTKTANVTVKRPRRVQLPATKASPASGHQLSDQEEADHGRGYEGDSSSGSGRSWGERSRRSRAGRRGRVGSHGRRSSGGGSEANGLDLVSGYKRLPKQDVLMRPLKSVLVKRRNSEEFGVKLGSQIFIKHITESGLAARNHGLQEGDLILQINGVSSANLSLSDTRRLIEKSEGELTLLVLRDSGQFLVNIPPAVSDSDSSLMEDISDLTSELSQAPPSHVPPPPLKGQRSPEDSQTDSPVETPQPRRRERSVNSRAIAEPESPGESRYDIYRVPSRQSLEDRGYSPDTRVVSFPKGASIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDEILQVNGMPFRNLTREEAVQFLLGLPPGEDMELVTQSKQDIFKKMVQSRVGDSFYIRTHFELEPSPPYGLGFTRGDVFHVVDTLYPGSGPGHGHSSHGGLWLAARMGRDLREQERGVIPNQSRAEQLASLEAAQRAAGVGPGASVGSNPRAEFWRLRGLRRGTKKASTQRSREDLSALTRQGHYPPYERVVLREASFKRPVVILGPVADIAMKKLTTEMPEEFEIAESMSRTDSPSKIIKLDTVRVIAERDKHALLDVTPSAIERLNYVQYYPIVIFCAPESRPALKALREWLAPASRRSSRRLYAQAQKLQKHSGHLFTATIPLHGTSDSWYQEVKAVIQQQQARPIWTAEDQLNSSSEDLDLPGHGLAASSGDLSCDSRTNSDYEDTDGEGAYTDREGGPQDVDEEVAPTALARSSEPVWVDDHQGLMGHGTTITDKWETQADSHYTQDQRRQDSMRTYKHEALRKKFTRARDVESSDDEGYDWGPATDL

Target ORF information:

RefSeq Version	NM_013769.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 3 (Tjp3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013769.3

ORF Insert Sequence:

ATGGAAGAGC TGACCATCTG GGAACAACAC ACAGCCACAC TGTACAAGGA CCCCCGCCGC 
GGCTTCGGCA TAGCTGTCTC TGGAGGCCAT GACCGAGCTA GTGGATCCGT GGTGGTGTCT 
GATGTGGTAC CAGGAGGCCC CGCTGAGGGC CGGCTCCGGA CCGGGGACCA CATCGTCATG 
GTGAATGGTG TCTCTGTGGA GAACGTCACA TCTGCCTTTG CCATCCAGAT CCTTAAGACC 
TGCACCAAAA CAGCCAACGT TACAGTGAAG CGTCCCCGCA GGGTCCAGCT GCCTGCCACC 
AAGGCTAGCC CAGCTTCTGG CCACCAGCTC TCAGACCAGG AAGAAGCTGA CCATGGCCGG 
GGCTATGAGG GGGACTCCTC CAGTGGCTCA GGTCGATCAT GGGGTGAGCG TTCTCGCCGG 
TCCAGGGCAG GTCGCCGCGG CAGGGTAGGC AGCCATGGGC GCCGGAGTTC GGGTGGCGGA 
TCTGAAGCCA ACGGTCTGGA CCTGGTGTCT GGCTATAAGC GCTTGCCGAA GCAGGATGTG 
CTAATGAGGC CACTAAAGTC CGTGCTGGTG AAACGAAGAA ACAGCGAAGA GTTTGGGGTG 
AAGCTAGGCA GTCAGATCTT TATAAAACAC ATCACGGAAT CTGGCCTTGC TGCTCGGAAC 
CATGGGCTGC AGGAGGGAGA CCTTATCTTA CAGATCAACG GGGTGTCCAG TGCTAATCTG 
TCCCTGAGTG ACACACGAAG GCTCATTGAG AAGTCGGAGG GGGAGCTGAC GTTGCTGGTG 
CTAAGAGACA GTGGGCAGTT CCTGGTGAAC ATCCCTCCCG CAGTGAGCGA CAGCGACAGC 
TCCCTAATGG AAGACATCTC AGACCTGACC TCAGAATTGT CCCAGGCGCC CCCTTCCCAC 
GTTCCTCCGC CACCTCTGAA GGGTCAGAGG AGCCCTGAGG ACAGCCAGAC AGACTCCCCA 
GTGGAGACCC CGCAGCCCCG GAGGCGGGAA CGGTCAGTGA ATTCAAGAGC CATCGCTGAA 
CCAGAGTCTC CTGGAGAAAG CCGCTATGAC ATTTACAGGG TTCCTAGCCG GCAGAGCCTG 
GAGGACCGTG GCTACAGCCC CGACACTCGT GTCGTGAGCT TCCCCAAGGG CGCGAGTATT 
GGCTTGCGGC TGGCCGGCGG CAACGACGTG GGCATCTTCG TATCCGGGGT GCAGGCAGGC 
AGCCCGGCCG ACGGGCAAGG CATCCAGGAA GGGGATGAGA TCTTGCAGGT GAATGGTATG 
CCATTTCGGA ACCTGACTCG GGAGGAGGCC GTGCAGTTTC TGTTGGGGCT GCCTCCAGGG 
GAAGACATGG AGCTGGTAAC ACAGAGCAAA CAGGACATCT TTAAGAAGAT GGTCCAGTCT 
CGCGTGGGTG ACTCCTTCTA CATCCGCACA CACTTTGAGC TGGAGCCGAG CCCACCCTAT 
GGCCTGGGCT TCACCCGCGG TGATGTCTTC CACGTAGTGG ACACGTTGTA CCCGGGCTCC 
GGGCCAGGCC ATGGGCACAG CAGCCATGGG GGCCTCTGGC TGGCCGCCCG CATGGGCAGA 
GATCTGCGAG AGCAAGAACG CGGCGTCATC CCCAACCAGA GCAGGGCCGA GCAGCTGGCC 
AGTCTGGAGG CTGCGCAGCG GGCCGCGGGC GTGGGGCCAG GCGCCTCAGT GGGTTCCAAC 
CCACGGGCGG AGTTTTGGCG ACTCCGAGGT CTCCGCCGAG GGACCAAGAA GGCGTCGACC 
CAGCGGAGCC GCGAGGACCT ATCAGCGCTG ACCAGGCAGG GACACTACCC GCCCTATGAG 
CGTGTGGTGC TCAGAGAAGC CAGTTTTAAG CGCCCCGTGG TGATCCTAGG ACCAGTGGCA 
GACATTGCCA TGAAGAAACT GACCACAGAG ATGCCAGAAG AGTTTGAGAT TGCAGAAAGC 
ATGTCCCGGA CTGACAGCCC ATCCAAGATC ATCAAACTGG ACACAGTGCG GGTGATCGCA 
GAGAGGGATA AGCACGCACT CCTGGATGTC ACCCCCTCTG CCATCGAGCG CCTCAACTAC 
GTGCAGTACT ACCCCATCGT CATCTTTTGT GCCCCAGAGA GCCGGCCGGC CCTCAAGGCC 
CTCCGGGAGT GGCTGGCCCC GGCCTCTCGC CGCAGCAGCC GCCGCCTGTA TGCGCAGGCC 
CAGAAACTGC AGAAACACAG TGGCCACCTC TTCACCGCCA CCATCCCCCT GCATGGCACT 
AGTGACTCCT GGTACCAGGA AGTCAAGGCT GTAATACAGC AACAGCAGGC GCGACCGATC 
TGGACCGCGG AGGACCAGCT GAACAGCTCA TCCGAGGACC TTGACCTGCC GGGCCATGGT 
CTGGCCGCCA GCTCTGGAGA CCTCAGCTGT GACAGTCGGA CCAACAGCGA CTATGAGGAC 
ACCGACGGCG AGGGCGCCTA TACGGACAGA GAGGGCGGAC CCCAGGATGT CGATGAGGAG 
GTCGCACCCA CAGCCCTGGC CCGGTCCTCA GAGCCCGTAT GGGTAGATGA TCACCAGGGG 
CTGATGGGTC ATGGGACAAC TATCACAGAC AAATGGGAGA CACAGGCTGA CAGCCACTAC 
ACCCAGGACC AGCGGCGCCA GGACAGCATG CGGACCTACA AGCACGAGGC CCTGAGGAAG 
AAGTTCACAA GAGCCCGGGA TGTGGAATCC TCTGATGACG AGGGCTATGA CTGGGGCCCA 
GCCACCGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp3 ( NM_001282095.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001269024.1 Mus musculus tight junction protein 3 (Tjp3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu09924
Clone ID Related Accession (Same CDS sequence)	NM_013769.3 , XM_006513708.3 , NM_001282095.1
Accession Version	NM_001282095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK167587.1, AK029083.1 and AV236903.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK167587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAGC TGACCATCTG GGAACAACAC ACAGCCACAC TGTACAAGGA CCCCCGCCGC 
GGCTTCGGCA TAGCTGTCTC TGGAGGCCAT GACCGAGCTA GTGGATCCGT GGTGGTGTCT 
GATGTGGTAC CAGGAGGCCC CGCTGAGGGC CGGCTCCGGA CCGGGGACCA CATCGTCATG 
GTGAATGGTG TCTCTGTGGA GAACGTCACA TCTGCCTTTG CCATCCAGAT CCTTAAGACC 
TGCACCAAAA CAGCCAACGT TACAGTGAAG CGTCCCCGCA GGGTCCAGCT GCCTGCCACC 
AAGGCTAGCC CAGCTTCTGG CCACCAGCTC TCAGACCAGG AAGAAGCTGA CCATGGCCGG 
GGCTATGAGG GGGACTCCTC CAGTGGCTCA GGTCGATCAT GGGGTGAGCG TTCTCGCCGG 
TCCAGGGCAG GTCGCCGCGG CAGGGTAGGC AGCCATGGGC GCCGGAGTTC GGGTGGCGGA 
TCTGAAGCCA ACGGTCTGGA CCTGGTGTCT GGCTATAAGC GCTTGCCGAA GCAGGATGTG 
CTAATGAGGC CACTAAAGTC CGTGCTGGTG AAACGAAGAA ACAGCGAAGA GTTTGGGGTG 
AAGCTAGGCA GTCAGATCTT TATAAAACAC ATCACGGAAT CTGGCCTTGC TGCTCGGAAC 
CATGGGCTGC AGGAGGGAGA CCTTATCTTA CAGATCAACG GGGTGTCCAG TGCTAATCTG 
TCCCTGAGTG ACACACGAAG GCTCATTGAG AAGTCGGAGG GGGAGCTGAC GTTGCTGGTG 
CTAAGAGACA GTGGGCAGTT CCTGGTGAAC ATCCCTCCCG CAGTGAGCGA CAGCGACAGC 
TCCCTAATGG AAGACATCTC AGACCTGACC TCAGAATTGT CCCAGGCGCC CCCTTCCCAC 
GTTCCTCCGC CACCTCTGAA GGGTCAGAGG AGCCCTGAGG ACAGCCAGAC AGACTCCCCA 
GTGGAGACCC CGCAGCCCCG GAGGCGGGAA CGGTCAGTGA ATTCAAGAGC CATCGCTGAA 
CCAGAGTCTC CTGGAGAAAG CCGCTATGAC ATTTACAGGG TTCCTAGCCG GCAGAGCCTG 
GAGGACCGTG GCTACAGCCC CGACACTCGT GTCGTGAGCT TCCCCAAGGG CGCGAGTATT 
GGCTTGCGGC TGGCCGGCGG CAACGACGTG GGCATCTTCG TATCCGGGGT GCAGGCAGGC 
AGCCCGGCCG ACGGGCAAGG CATCCAGGAA GGGGATGAGA TCTTGCAGGT GAATGGTATG 
CCATTTCGGA ACCTGACTCG GGAGGAGGCC GTGCAGTTTC TGTTGGGGCT GCCTCCAGGG 
GAAGACATGG AGCTGGTAAC ACAGAGCAAA CAGGACATCT TTAAGAAGAT GGTCCAGTCT 
CGCGTGGGTG ACTCCTTCTA CATCCGCACA CACTTTGAGC TGGAGCCGAG CCCACCCTAT 
GGCCTGGGCT TCACCCGCGG TGATGTCTTC CACGTAGTGG ACACGTTGTA CCCGGGCTCC 
GGGCCAGGCC ATGGGCACAG CAGCCATGGG GGCCTCTGGC TGGCCGCCCG CATGGGCAGA 
GATCTGCGAG AGCAAGAACG CGGCGTCATC CCCAACCAGA GCAGGGCCGA GCAGCTGGCC 
AGTCTGGAGG CTGCGCAGCG GGCCGCGGGC GTGGGGCCAG GCGCCTCAGT GGGTTCCAAC 
CCACGGGCGG AGTTTTGGCG ACTCCGAGGT CTCCGCCGAG GGACCAAGAA GGCGTCGACC 
CAGCGGAGCC GCGAGGACCT ATCAGCGCTG ACCAGGCAGG GACACTACCC GCCCTATGAG 
CGTGTGGTGC TCAGAGAAGC CAGTTTTAAG CGCCCCGTGG TGATCCTAGG ACCAGTGGCA 
GACATTGCCA TGAAGAAACT GACCACAGAG ATGCCAGAAG AGTTTGAGAT TGCAGAAAGC 
ATGTCCCGGA CTGACAGCCC ATCCAAGATC ATCAAACTGG ACACAGTGCG GGTGATCGCA 
GAGAGGGATA AGCACGCACT CCTGGATGTC ACCCCCTCTG CCATCGAGCG CCTCAACTAC 
GTGCAGTACT ACCCCATCGT CATCTTTTGT GCCCCAGAGA GCCGGCCGGC CCTCAAGGCC 
CTCCGGGAGT GGCTGGCCCC GGCCTCTCGC CGCAGCAGCC GCCGCCTGTA TGCGCAGGCC 
CAGAAACTGC AGAAACACAG TGGCCACCTC TTCACCGCCA CCATCCCCCT GCATGGCACT 
AGTGACTCCT GGTACCAGGA AGTCAAGGCT GTAATACAGC AACAGCAGGC GCGACCGATC 
TGGACCGCGG AGGACCAGCT GAACAGCTCA TCCGAGGACC TTGACCTGCC GGGCCATGGT 
CTGGCCGCCA GCTCTGGAGA CCTCAGCTGT GACAGTCGGA CCAACAGCGA CTATGAGGAC 
ACCGACGGCG AGGGCGCCTA TACGGACAGA GAGGGCGGAC CCCAGGATGT CGATGAGGAG 
GTCGCACCCA CAGCCCTGGC CCGGTCCTCA GAGCCCGTAT GGGTAGATGA TCACCAGGGG 
CTGATGGGTC ATGGGACAAC TATCACAGAC AAATGGGAGA CACAGGCTGA CAGCCACTAC 
ACCCAGGACC AGCGGCGCCA GGACAGCATG CGGACCTACA AGCACGAGGC CCTGAGGAAG 
AAGTTCACAA GAGCCCGGGA TGTGGAATCC TCTGATGACG AGGGCTATGA CTGGGGCCCA 
GCCACCGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269024.1
CDS	128..2842
Translation	MEELTIWEQHTATLYKDPRRGFGIAVSGGHDRASGSVVVSDVVPGGPAEGRLRTGDHIVMVNGVSVENVTSAFAIQILKTCTKTANVTVKRPRRVQLPATKASPASGHQLSDQEEADHGRGYEGDSSSGSGRSWGERSRRSRAGRRGRVGSHGRRSSGGGSEANGLDLVSGYKRLPKQDVLMRPLKSVLVKRRNSEEFGVKLGSQIFIKHITESGLAARNHGLQEGDLILQINGVSSANLSLSDTRRLIEKSEGELTLLVLRDSGQFLVNIPPAVSDSDSSLMEDISDLTSELSQAPPSHVPPPPLKGQRSPEDSQTDSPVETPQPRRRERSVNSRAIAEPESPGESRYDIYRVPSRQSLEDRGYSPDTRVVSFPKGASIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDEILQVNGMPFRNLTREEAVQFLLGLPPGEDMELVTQSKQDIFKKMVQSRVGDSFYIRTHFELEPSPPYGLGFTRGDVFHVVDTLYPGSGPGHGHSSHGGLWLAARMGRDLREQERGVIPNQSRAEQLASLEAAQRAAGVGPGASVGSNPRAEFWRLRGLRRGTKKASTQRSREDLSALTRQGHYPPYERVVLREASFKRPVVILGPVADIAMKKLTTEMPEEFEIAESMSRTDSPSKIIKLDTVRVIAERDKHALLDVTPSAIERLNYVQYYPIVIFCAPESRPALKALREWLAPASRRSSRRLYAQAQKLQKHSGHLFTATIPLHGTSDSWYQEVKAVIQQQQARPIWTAEDQLNSSSEDLDLPGHGLAASSGDLSCDSRTNSDYEDTDGEGAYTDREGGPQDVDEEVAPTALARSSEPVWVDDHQGLMGHGTTITDKWETQADSHYTQDQRRQDSMRTYKHEALRKKFTRARDVESSDDEGYDWGPATDL

Target ORF information:

RefSeq Version	NM_001282095.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 3 (Tjp3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282095.1

ORF Insert Sequence:

ATGGAAGAGC TGACCATCTG GGAACAACAC ACAGCCACAC TGTACAAGGA CCCCCGCCGC 
GGCTTCGGCA TAGCTGTCTC TGGAGGCCAT GACCGAGCTA GTGGATCCGT GGTGGTGTCT 
GATGTGGTAC CAGGAGGCCC CGCTGAGGGC CGGCTCCGGA CCGGGGACCA CATCGTCATG 
GTGAATGGTG TCTCTGTGGA GAACGTCACA TCTGCCTTTG CCATCCAGAT CCTTAAGACC 
TGCACCAAAA CAGCCAACGT TACAGTGAAG CGTCCCCGCA GGGTCCAGCT GCCTGCCACC 
AAGGCTAGCC CAGCTTCTGG CCACCAGCTC TCAGACCAGG AAGAAGCTGA CCATGGCCGG 
GGCTATGAGG GGGACTCCTC CAGTGGCTCA GGTCGATCAT GGGGTGAGCG TTCTCGCCGG 
TCCAGGGCAG GTCGCCGCGG CAGGGTAGGC AGCCATGGGC GCCGGAGTTC GGGTGGCGGA 
TCTGAAGCCA ACGGTCTGGA CCTGGTGTCT GGCTATAAGC GCTTGCCGAA GCAGGATGTG 
CTAATGAGGC CACTAAAGTC CGTGCTGGTG AAACGAAGAA ACAGCGAAGA GTTTGGGGTG 
AAGCTAGGCA GTCAGATCTT TATAAAACAC ATCACGGAAT CTGGCCTTGC TGCTCGGAAC 
CATGGGCTGC AGGAGGGAGA CCTTATCTTA CAGATCAACG GGGTGTCCAG TGCTAATCTG 
TCCCTGAGTG ACACACGAAG GCTCATTGAG AAGTCGGAGG GGGAGCTGAC GTTGCTGGTG 
CTAAGAGACA GTGGGCAGTT CCTGGTGAAC ATCCCTCCCG CAGTGAGCGA CAGCGACAGC 
TCCCTAATGG AAGACATCTC AGACCTGACC TCAGAATTGT CCCAGGCGCC CCCTTCCCAC 
GTTCCTCCGC CACCTCTGAA GGGTCAGAGG AGCCCTGAGG ACAGCCAGAC AGACTCCCCA 
GTGGAGACCC CGCAGCCCCG GAGGCGGGAA CGGTCAGTGA ATTCAAGAGC CATCGCTGAA 
CCAGAGTCTC CTGGAGAAAG CCGCTATGAC ATTTACAGGG TTCCTAGCCG GCAGAGCCTG 
GAGGACCGTG GCTACAGCCC CGACACTCGT GTCGTGAGCT TCCCCAAGGG CGCGAGTATT 
GGCTTGCGGC TGGCCGGCGG CAACGACGTG GGCATCTTCG TATCCGGGGT GCAGGCAGGC 
AGCCCGGCCG ACGGGCAAGG CATCCAGGAA GGGGATGAGA TCTTGCAGGT GAATGGTATG 
CCATTTCGGA ACCTGACTCG GGAGGAGGCC GTGCAGTTTC TGTTGGGGCT GCCTCCAGGG 
GAAGACATGG AGCTGGTAAC ACAGAGCAAA CAGGACATCT TTAAGAAGAT GGTCCAGTCT 
CGCGTGGGTG ACTCCTTCTA CATCCGCACA CACTTTGAGC TGGAGCCGAG CCCACCCTAT 
GGCCTGGGCT TCACCCGCGG TGATGTCTTC CACGTAGTGG ACACGTTGTA CCCGGGCTCC 
GGGCCAGGCC ATGGGCACAG CAGCCATGGG GGCCTCTGGC TGGCCGCCCG CATGGGCAGA 
GATCTGCGAG AGCAAGAACG CGGCGTCATC CCCAACCAGA GCAGGGCCGA GCAGCTGGCC 
AGTCTGGAGG CTGCGCAGCG GGCCGCGGGC GTGGGGCCAG GCGCCTCAGT GGGTTCCAAC 
CCACGGGCGG AGTTTTGGCG ACTCCGAGGT CTCCGCCGAG GGACCAAGAA GGCGTCGACC 
CAGCGGAGCC GCGAGGACCT ATCAGCGCTG ACCAGGCAGG GACACTACCC GCCCTATGAG 
CGTGTGGTGC TCAGAGAAGC CAGTTTTAAG CGCCCCGTGG TGATCCTAGG ACCAGTGGCA 
GACATTGCCA TGAAGAAACT GACCACAGAG ATGCCAGAAG AGTTTGAGAT TGCAGAAAGC 
ATGTCCCGGA CTGACAGCCC ATCCAAGATC ATCAAACTGG ACACAGTGCG GGTGATCGCA 
GAGAGGGATA AGCACGCACT CCTGGATGTC ACCCCCTCTG CCATCGAGCG CCTCAACTAC 
GTGCAGTACT ACCCCATCGT CATCTTTTGT GCCCCAGAGA GCCGGCCGGC CCTCAAGGCC 
CTCCGGGAGT GGCTGGCCCC GGCCTCTCGC CGCAGCAGCC GCCGCCTGTA TGCGCAGGCC 
CAGAAACTGC AGAAACACAG TGGCCACCTC TTCACCGCCA CCATCCCCCT GCATGGCACT 
AGTGACTCCT GGTACCAGGA AGTCAAGGCT GTAATACAGC AACAGCAGGC GCGACCGATC 
TGGACCGCGG AGGACCAGCT GAACAGCTCA TCCGAGGACC TTGACCTGCC GGGCCATGGT 
CTGGCCGCCA GCTCTGGAGA CCTCAGCTGT GACAGTCGGA CCAACAGCGA CTATGAGGAC 
ACCGACGGCG AGGGCGCCTA TACGGACAGA GAGGGCGGAC CCCAGGATGT CGATGAGGAG 
GTCGCACCCA CAGCCCTGGC CCGGTCCTCA GAGCCCGTAT GGGTAGATGA TCACCAGGGG 
CTGATGGGTC ATGGGACAAC TATCACAGAC AAATGGGAGA CACAGGCTGA CAGCCACTAC 
ACCCAGGACC AGCGGCGCCA GGACAGCATG CGGACCTACA AGCACGAGGC CCTGAGGAAG 
AAGTTCACAA GAGCCCGGGA TGTGGAATCC TCTGATGACG AGGGCTATGA CTGGGGCCCA 
GCCACCGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp3 ( XM_006513708.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513771.1 Mus musculus tight junction protein 3 (Tjp3), transcript variant X1, mRNA.

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CloneID	OMu09924
Clone ID Related Accession (Same CDS sequence)	NM_013769.3 , XM_006513708.3 , NM_001282095.1
Accession Version	XM_006513708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGC TGACCATCTG GGAACAACAC ACAGCCACAC TGTACAAGGA CCCCCGCCGC 
GGCTTCGGCA TAGCTGTCTC TGGAGGCCAT GACCGAGCTA GTGGATCCGT GGTGGTGTCT 
GATGTGGTAC CAGGAGGCCC CGCTGAGGGC CGGCTCCGGA CCGGGGACCA CATCGTCATG 
GTGAATGGTG TCTCTGTGGA GAACGTCACA TCTGCCTTTG CCATCCAGAT CCTTAAGACC 
TGCACCAAAA CAGCCAACGT TACAGTGAAG CGTCCCCGCA GGGTCCAGCT GCCTGCCACC 
AAGGCTAGCC CAGCTTCTGG CCACCAGCTC TCAGACCAGG AAGAAGCTGA CCATGGCCGG 
GGCTATGAGG GGGACTCCTC CAGTGGCTCA GGTCGATCAT GGGGTGAGCG TTCTCGCCGG 
TCCAGGGCAG GTCGCCGCGG CAGGGTAGGC AGCCATGGGC GCCGGAGTTC GGGTGGCGGA 
TCTGAAGCCA ACGGTCTGGA CCTGGTGTCT GGCTATAAGC GCTTGCCGAA GCAGGATGTG 
CTAATGAGGC CACTAAAGTC CGTGCTGGTG AAACGAAGAA ACAGCGAAGA GTTTGGGGTG 
AAGCTAGGCA GTCAGATCTT TATAAAACAC ATCACGGAAT CTGGCCTTGC TGCTCGGAAC 
CATGGGCTGC AGGAGGGAGA CCTTATCTTA CAGATCAACG GGGTGTCCAG TGCTAATCTG 
TCCCTGAGTG ACACACGAAG GCTCATTGAG AAGTCGGAGG GGGAGCTGAC GTTGCTGGTG 
CTAAGAGACA GTGGGCAGTT CCTGGTGAAC ATCCCTCCCG CAGTGAGCGA CAGCGACAGC 
TCCCTAATGG AAGACATCTC AGACCTGACC TCAGAATTGT CCCAGGCGCC CCCTTCCCAC 
GTTCCTCCGC CACCTCTGAA GGGTCAGAGG AGCCCTGAGG ACAGCCAGAC AGACTCCCCA 
GTGGAGACCC CGCAGCCCCG GAGGCGGGAA CGGTCAGTGA ATTCAAGAGC CATCGCTGAA 
CCAGAGTCTC CTGGAGAAAG CCGCTATGAC ATTTACAGGG TTCCTAGCCG GCAGAGCCTG 
GAGGACCGTG GCTACAGCCC CGACACTCGT GTCGTGAGCT TCCCCAAGGG CGCGAGTATT 
GGCTTGCGGC TGGCCGGCGG CAACGACGTG GGCATCTTCG TATCCGGGGT GCAGGCAGGC 
AGCCCGGCCG ACGGGCAAGG CATCCAGGAA GGGGATGAGA TCTTGCAGGT GAATGGTATG 
CCATTTCGGA ACCTGACTCG GGAGGAGGCC GTGCAGTTTC TGTTGGGGCT GCCTCCAGGG 
GAAGACATGG AGCTGGTAAC ACAGAGCAAA CAGGACATCT TTAAGAAGAT GGTCCAGTCT 
CGCGTGGGTG ACTCCTTCTA CATCCGCACA CACTTTGAGC TGGAGCCGAG CCCACCCTAT 
GGCCTGGGCT TCACCCGCGG TGATGTCTTC CACGTAGTGG ACACGTTGTA CCCGGGCTCC 
GGGCCAGGCC ATGGGCACAG CAGCCATGGG GGCCTCTGGC TGGCCGCCCG CATGGGCAGA 
GATCTGCGAG AGCAAGAACG CGGCGTCATC CCCAACCAGA GCAGGGCCGA GCAGCTGGCC 
AGTCTGGAGG CTGCGCAGCG GGCCGCGGGC GTGGGGCCAG GCGCCTCAGT GGGTTCCAAC 
CCACGGGCGG AGTTTTGGCG ACTCCGAGGT CTCCGCCGAG GGACCAAGAA GGCGTCGACC 
CAGCGGAGCC GCGAGGACCT ATCAGCGCTG ACCAGGCAGG GACACTACCC GCCCTATGAG 
CGTGTGGTGC TCAGAGAAGC CAGTTTTAAG CGCCCCGTGG TGATCCTAGG ACCAGTGGCA 
GACATTGCCA TGAAGAAACT GACCACAGAG ATGCCAGAAG AGTTTGAGAT TGCAGAAAGC 
ATGTCCCGGA CTGACAGCCC ATCCAAGATC ATCAAACTGG ACACAGTGCG GGTGATCGCA 
GAGAGGGATA AGCACGCACT CCTGGATGTC ACCCCCTCTG CCATCGAGCG CCTCAACTAC 
GTGCAGTACT ACCCCATCGT CATCTTTTGT GCCCCAGAGA GCCGGCCGGC CCTCAAGGCC 
CTCCGGGAGT GGCTGGCCCC GGCCTCTCGC CGCAGCAGCC GCCGCCTGTA TGCGCAGGCC 
CAGAAACTGC AGAAACACAG TGGCCACCTC TTCACCGCCA CCATCCCCCT GCATGGCACT 
AGTGACTCCT GGTACCAGGA AGTCAAGGCT GTAATACAGC AACAGCAGGC GCGACCGATC 
TGGACCGCGG AGGACCAGCT GAACAGCTCA TCCGAGGACC TTGACCTGCC GGGCCATGGT 
CTGGCCGCCA GCTCTGGAGA CCTCAGCTGT GACAGTCGGA CCAACAGCGA CTATGAGGAC 
ACCGACGGCG AGGGCGCCTA TACGGACAGA GAGGGCGGAC CCCAGGATGT CGATGAGGAG 
GTCGCACCCA CAGCCCTGGC CCGGTCCTCA GAGCCCGTAT GGGTAGATGA TCACCAGGGG 
CTGATGGGTC ATGGGACAAC TATCACAGAC AAATGGGAGA CACAGGCTGA CAGCCACTAC 
ACCCAGGACC AGCGGCGCCA GGACAGCATG CGGACCTACA AGCACGAGGC CCTGAGGAAG 
AAGTTCACAA GAGCCCGGGA TGTGGAATCC TCTGATGACG AGGGCTATGA CTGGGGCCCA 
GCCACCGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513771.1
CDS	307..3021
Translation	MEELTIWEQHTATLYKDPRRGFGIAVSGGHDRASGSVVVSDVVPGGPAEGRLRTGDHIVMVNGVSVENVTSAFAIQILKTCTKTANVTVKRPRRVQLPATKASPASGHQLSDQEEADHGRGYEGDSSSGSGRSWGERSRRSRAGRRGRVGSHGRRSSGGGSEANGLDLVSGYKRLPKQDVLMRPLKSVLVKRRNSEEFGVKLGSQIFIKHITESGLAARNHGLQEGDLILQINGVSSANLSLSDTRRLIEKSEGELTLLVLRDSGQFLVNIPPAVSDSDSSLMEDISDLTSELSQAPPSHVPPPPLKGQRSPEDSQTDSPVETPQPRRRERSVNSRAIAEPESPGESRYDIYRVPSRQSLEDRGYSPDTRVVSFPKGASIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDEILQVNGMPFRNLTREEAVQFLLGLPPGEDMELVTQSKQDIFKKMVQSRVGDSFYIRTHFELEPSPPYGLGFTRGDVFHVVDTLYPGSGPGHGHSSHGGLWLAARMGRDLREQERGVIPNQSRAEQLASLEAAQRAAGVGPGASVGSNPRAEFWRLRGLRRGTKKASTQRSREDLSALTRQGHYPPYERVVLREASFKRPVVILGPVADIAMKKLTTEMPEEFEIAESMSRTDSPSKIIKLDTVRVIAERDKHALLDVTPSAIERLNYVQYYPIVIFCAPESRPALKALREWLAPASRRSSRRLYAQAQKLQKHSGHLFTATIPLHGTSDSWYQEVKAVIQQQQARPIWTAEDQLNSSSEDLDLPGHGLAASSGDLSCDSRTNSDYEDTDGEGAYTDREGGPQDVDEEVAPTALARSSEPVWVDDHQGLMGHGTTITDKWETQADSHYTQDQRRQDSMRTYKHEALRKKFTRARDVESSDDEGYDWGPATDL

Target ORF information:

RefSeq Version	XM_006513708.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 3 (Tjp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513708.3

ORF Insert Sequence:

ATGGAAGAGC TGACCATCTG GGAACAACAC ACAGCCACAC TGTACAAGGA CCCCCGCCGC 
GGCTTCGGCA TAGCTGTCTC TGGAGGCCAT GACCGAGCTA GTGGATCCGT GGTGGTGTCT 
GATGTGGTAC CAGGAGGCCC CGCTGAGGGC CGGCTCCGGA CCGGGGACCA CATCGTCATG 
GTGAATGGTG TCTCTGTGGA GAACGTCACA TCTGCCTTTG CCATCCAGAT CCTTAAGACC 
TGCACCAAAA CAGCCAACGT TACAGTGAAG CGTCCCCGCA GGGTCCAGCT GCCTGCCACC 
AAGGCTAGCC CAGCTTCTGG CCACCAGCTC TCAGACCAGG AAGAAGCTGA CCATGGCCGG 
GGCTATGAGG GGGACTCCTC CAGTGGCTCA GGTCGATCAT GGGGTGAGCG TTCTCGCCGG 
TCCAGGGCAG GTCGCCGCGG CAGGGTAGGC AGCCATGGGC GCCGGAGTTC GGGTGGCGGA 
TCTGAAGCCA ACGGTCTGGA CCTGGTGTCT GGCTATAAGC GCTTGCCGAA GCAGGATGTG 
CTAATGAGGC CACTAAAGTC CGTGCTGGTG AAACGAAGAA ACAGCGAAGA GTTTGGGGTG 
AAGCTAGGCA GTCAGATCTT TATAAAACAC ATCACGGAAT CTGGCCTTGC TGCTCGGAAC 
CATGGGCTGC AGGAGGGAGA CCTTATCTTA CAGATCAACG GGGTGTCCAG TGCTAATCTG 
TCCCTGAGTG ACACACGAAG GCTCATTGAG AAGTCGGAGG GGGAGCTGAC GTTGCTGGTG 
CTAAGAGACA GTGGGCAGTT CCTGGTGAAC ATCCCTCCCG CAGTGAGCGA CAGCGACAGC 
TCCCTAATGG AAGACATCTC AGACCTGACC TCAGAATTGT CCCAGGCGCC CCCTTCCCAC 
GTTCCTCCGC CACCTCTGAA GGGTCAGAGG AGCCCTGAGG ACAGCCAGAC AGACTCCCCA 
GTGGAGACCC CGCAGCCCCG GAGGCGGGAA CGGTCAGTGA ATTCAAGAGC CATCGCTGAA 
CCAGAGTCTC CTGGAGAAAG CCGCTATGAC ATTTACAGGG TTCCTAGCCG GCAGAGCCTG 
GAGGACCGTG GCTACAGCCC CGACACTCGT GTCGTGAGCT TCCCCAAGGG CGCGAGTATT 
GGCTTGCGGC TGGCCGGCGG CAACGACGTG GGCATCTTCG TATCCGGGGT GCAGGCAGGC 
AGCCCGGCCG ACGGGCAAGG CATCCAGGAA GGGGATGAGA TCTTGCAGGT GAATGGTATG 
CCATTTCGGA ACCTGACTCG GGAGGAGGCC GTGCAGTTTC TGTTGGGGCT GCCTCCAGGG 
GAAGACATGG AGCTGGTAAC ACAGAGCAAA CAGGACATCT TTAAGAAGAT GGTCCAGTCT 
CGCGTGGGTG ACTCCTTCTA CATCCGCACA CACTTTGAGC TGGAGCCGAG CCCACCCTAT 
GGCCTGGGCT TCACCCGCGG TGATGTCTTC CACGTAGTGG ACACGTTGTA CCCGGGCTCC 
GGGCCAGGCC ATGGGCACAG CAGCCATGGG GGCCTCTGGC TGGCCGCCCG CATGGGCAGA 
GATCTGCGAG AGCAAGAACG CGGCGTCATC CCCAACCAGA GCAGGGCCGA GCAGCTGGCC 
AGTCTGGAGG CTGCGCAGCG GGCCGCGGGC GTGGGGCCAG GCGCCTCAGT GGGTTCCAAC 
CCACGGGCGG AGTTTTGGCG ACTCCGAGGT CTCCGCCGAG GGACCAAGAA GGCGTCGACC 
CAGCGGAGCC GCGAGGACCT ATCAGCGCTG ACCAGGCAGG GACACTACCC GCCCTATGAG 
CGTGTGGTGC TCAGAGAAGC CAGTTTTAAG CGCCCCGTGG TGATCCTAGG ACCAGTGGCA 
GACATTGCCA TGAAGAAACT GACCACAGAG ATGCCAGAAG AGTTTGAGAT TGCAGAAAGC 
ATGTCCCGGA CTGACAGCCC ATCCAAGATC ATCAAACTGG ACACAGTGCG GGTGATCGCA 
GAGAGGGATA AGCACGCACT CCTGGATGTC ACCCCCTCTG CCATCGAGCG CCTCAACTAC 
GTGCAGTACT ACCCCATCGT CATCTTTTGT GCCCCAGAGA GCCGGCCGGC CCTCAAGGCC 
CTCCGGGAGT GGCTGGCCCC GGCCTCTCGC CGCAGCAGCC GCCGCCTGTA TGCGCAGGCC 
CAGAAACTGC AGAAACACAG TGGCCACCTC TTCACCGCCA CCATCCCCCT GCATGGCACT 
AGTGACTCCT GGTACCAGGA AGTCAAGGCT GTAATACAGC AACAGCAGGC GCGACCGATC 
TGGACCGCGG AGGACCAGCT GAACAGCTCA TCCGAGGACC TTGACCTGCC GGGCCATGGT 
CTGGCCGCCA GCTCTGGAGA CCTCAGCTGT GACAGTCGGA CCAACAGCGA CTATGAGGAC 
ACCGACGGCG AGGGCGCCTA TACGGACAGA GAGGGCGGAC CCCAGGATGT CGATGAGGAG 
GTCGCACCCA CAGCCCTGGC CCGGTCCTCA GAGCCCGTAT GGGTAGATGA TCACCAGGGG 
CTGATGGGTC ATGGGACAAC TATCACAGAC AAATGGGAGA CACAGGCTGA CAGCCACTAC 
ACCCAGGACC AGCGGCGCCA GGACAGCATG CGGACCTACA AGCACGAGGC CCTGAGGAAG 
AAGTTCACAA GAGCCCGGGA TGTGGAATCC TCTGATGACG AGGGCTATGA CTGGGGCCCA 
GCCACCGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tjp3 ( NM_001282096.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001269025.1 Mus musculus tight junction protein 3 (Tjp3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08435
Clone ID Related Accession (Same CDS sequence)	NM_001282096.1
Accession Version	NM_001282096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	tight junction protein ZO-3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC155937.4, BY102203.1, AK029083.1 and AV236903.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGA GATTCCAGGT GGCAGACATG GAAGAGCTGA CCATCTGGGA ACAACACACA 
GCCACACTGT ACAAGGACCC CCGCCGCGGC TTCGGCATAG CTGTCTCTGG AGGCCATGAC 
CGAGCTAGTG GATCCGTGGT GGTGTCTGAT GTGGTACCAG GAGGCCCCGC TGAGGGCCGG 
CTCCGGACCG GGGACCACAT CGTCATGGTG AATGGTGTCT CTGTGGAGAA CGTCACATCT 
GCCTTTGCCA TCCAGATCCT TAAGACCTGC ACCAAAACAG CCAACGTTAC AGTGAAGCGT 
CCCCGCAGGG TCCAGCTGCC TGCCACCAAG GCTAGCCCAG CTTCTGGCCA CCAGCTCTCA 
GACCAGGAAG AAGCTGACCA TGGCCGGGGC TATGAGGGGG ACTCCTCCAG TGGCTCAGGT 
CGATCATGGG GTGAGCGTTC TCGCCGGTCC AGGGCAGGTC GCCGCGGCAG GGTAGGCAGC 
CATGGGCGCC GGAGTTCGGG TGGCGGATCT GAAGCCAACG GTCTGGACCT GGTGTCTGGC 
TATAAGCGCT TGCCGAAGCA GGATGTGCTA ATGAGGCCAC TAAAGTCCGT GCTGGTGAAA 
CGAAGAAACA GCGAAGAGTT TGGGGTGAAG CTAGGCAGTC AGATCTTTAT AAAACACATC 
ACGGAATCTG GCCTTGCTGC TCGGAACCAT GGGCTGCAGG AGGGAGACCT TATCTTACAG 
ATCAACGGGG TGTCCAGTGC TAATCTGTCC CTGAGTGACA CACGAAGGCT CATTGAGAAG 
TCGGAGGGGG AGCTGACGTT GCTGGTGCTA AGAGACAGTG GGCAGTTCCT GGTGAACATC 
CCTCCCGCAG TGAGCGACAG CGACAGCTCC CTAATGGAAG ACATCTCAGA CCTGACCTCA 
GAATTGTCCC AGGCGCCCCC TTCCCACGTT CCTCCGCCAC CTCTGAAGGG TCAGAGGAGC 
CCTGAGGACA GCCAGACAGA CTCCCCAGTG GAGACCCCGC AGCCCCGGAG GCGGGAACGG 
TCAGTGAATT CAAGAGCCAT CGCTGAACCA GAGTCTCCTG GAGAAAGCCG CTATGACATT 
TACAGGGTTC CTAGCCGGCA GAGCCTGGAG GACCGTGGCT ACAGCCCCGA CACTCGTGTC 
GTGAGCTTCC CCAAGGGCGC GAGTATTGGC TTGCGGCTGG CCGGCGGCAA CGACGTGGGC 
ATCTTCGTAT CCGGGGTGCA GGCAGGCAGC CCGGCCGACG GGCAAGGCAT CCAGGAAGGG 
GATGAGATCT TGCAGGTGAA TGGTATGCCA TTTCGGAACC TGACTCGGGA GGAGGCCGTG 
CAGTTTCTGT TGGGGCTGCC TCCAGGGGAA GACATGGAGC TGGTAACACA GAGCAAACAG 
GACATCTTTA AGAAGATGGT CCAGTCTCGC GTGGGTGACT CCTTCTACAT CCGCACACAC 
TTTGAGCTGG AGCCGAGCCC ACCCTATGGC CTGGGCTTCA CCCGCGGTGA TGTCTTCCAC 
GTAGTGGACA CGTTGTACCC GGGCTCCGGG CCAGGCCATG GGCACAGCAG CCATGGGGGC 
CTCTGGCTGG CCGCCCGCAT GGGCAGAGAT CTGCGAGAGC AAGAACGCGG CGTCATCCCC 
AACCAGAGCA GGGCCGAGCA GCTGGCCAGT CTGGAGGCTG CGCAGCGGGC CGCGGGCGTG 
GGGCCAGGCG CCTCAGTGGG TTCCAACCCA CGGGCGGAGT TTTGGCGACT CCGAGGTCTC 
CGCCGAGGGA CCAAGAAGGC GTCGACCCAG CGGAGCCGCG AGGACCTATC AGCGCTGACC 
AGGCAGGGAC ACTACCCGCC CTATGAGCGT GTGGTGCTCA GAGAAGCCAG TTTTAAGCGC 
CCCGTGGTGA TCCTAGGACC AGTGGCAGAC ATTGCCATGA AGAAACTGAC CACAGAGATG 
CCAGAAGAGT TTGAGATTGC AGAAAGCATG TCCCGGACTG ACAGCCCATC CAAGATCATC 
AAACTGGACA CAGTGCGGGT GATCGCAGAG AGGGATAAGC ACGCACTCCT GGATGTCACC 
CCCTCTGCCA TCGAGCGCCT CAACTACGTG CAGTACTACC CCATCGTCAT CTTTTGTGCC 
CCAGAGAGCC GGCCGGCCCT CAAGGCCCTC CGGGAGTGGC TGGCCCCGGC CTCTCGCCGC 
AGCAGCCGCC GCCTGTATGC GCAGGCCCAG AAACTGCAGA AACACAGTGG CCACCTCTTC 
ACCGCCACCA TCCCCCTGCA TGGCACTAGT GACTCCTGGT ACCAGGAAGT CAAGGCTGTA 
ATACAGCAAC AGCAGGCGCG ACCGATCTGG ACCGCGGAGG ACCAGCTGAA CAGCTCATCC 
GAGGACCTTG ACCTGCCGGG CCATGGTCTG GCCGCCAGCT CTGGAGACCT CAGCTGTGAC 
AGTCGGACCA ACAGCGACTA TGAGGACACC GACGGCGAGG GCGCCTATAC GGACAGAGAG 
GGCGGACCCC AGGATGTCGA TGAGGAGGTC GCACCCACAG CCCTGGCCCG GTCCTCAGAG 
CCCGTATGGG TAGATGATCA CCAGGGGCTG ATGGGTCATG GGACAACTAT CACAGACAAA 
TGGGAGACAC AGGCTGACAG CCACTACACC CAGGACCAGC GGCGCCAGGA CAGCATGCGG 
ACCTACAAGC ACGAGGCCCT GAGGAAGAAG TTCACAAGAG CCCGGGATGT GGAATCCTCT 
GATGACGAGG GCTATGACTG GGGCCCAGCC ACCGACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269025.1
CDS	164..2905
Translation	MAVRFQVADMEELTIWEQHTATLYKDPRRGFGIAVSGGHDRASGSVVVSDVVPGGPAEGRLRTGDHIVMVNGVSVENVTSAFAIQILKTCTKTANVTVKRPRRVQLPATKASPASGHQLSDQEEADHGRGYEGDSSSGSGRSWGERSRRSRAGRRGRVGSHGRRSSGGGSEANGLDLVSGYKRLPKQDVLMRPLKSVLVKRRNSEEFGVKLGSQIFIKHITESGLAARNHGLQEGDLILQINGVSSANLSLSDTRRLIEKSEGELTLLVLRDSGQFLVNIPPAVSDSDSSLMEDISDLTSELSQAPPSHVPPPPLKGQRSPEDSQTDSPVETPQPRRRERSVNSRAIAEPESPGESRYDIYRVPSRQSLEDRGYSPDTRVVSFPKGASIGLRLAGGNDVGIFVSGVQAGSPADGQGIQEGDEILQVNGMPFRNLTREEAVQFLLGLPPGEDMELVTQSKQDIFKKMVQSRVGDSFYIRTHFELEPSPPYGLGFTRGDVFHVVDTLYPGSGPGHGHSSHGGLWLAARMGRDLREQERGVIPNQSRAEQLASLEAAQRAAGVGPGASVGSNPRAEFWRLRGLRRGTKKASTQRSREDLSALTRQGHYPPYERVVLREASFKRPVVILGPVADIAMKKLTTEMPEEFEIAESMSRTDSPSKIIKLDTVRVIAERDKHALLDVTPSAIERLNYVQYYPIVIFCAPESRPALKALREWLAPASRRSSRRLYAQAQKLQKHSGHLFTATIPLHGTSDSWYQEVKAVIQQQQARPIWTAEDQLNSSSEDLDLPGHGLAASSGDLSCDSRTNSDYEDTDGEGAYTDREGGPQDVDEEVAPTALARSSEPVWVDDHQGLMGHGTTITDKWETQADSHYTQDQRRQDSMRTYKHEALRKKFTRARDVESSDDEGYDWGPATDL

Target ORF information:

RefSeq Version	NM_001282096.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tight junction protein 3 (Tjp3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282096.1

ORF Insert Sequence:

ATGGCGGTGA GATTCCAGGT GGCAGACATG GAAGAGCTGA CCATCTGGGA ACAACACACA 
GCCACACTGT ACAAGGACCC CCGCCGCGGC TTCGGCATAG CTGTCTCTGG AGGCCATGAC 
CGAGCTAGTG GATCCGTGGT GGTGTCTGAT GTGGTACCAG GAGGCCCCGC TGAGGGCCGG 
CTCCGGACCG GGGACCACAT CGTCATGGTG AATGGTGTCT CTGTGGAGAA CGTCACATCT 
GCCTTTGCCA TCCAGATCCT TAAGACCTGC ACCAAAACAG CCAACGTTAC AGTGAAGCGT 
CCCCGCAGGG TCCAGCTGCC TGCCACCAAG GCTAGCCCAG CTTCTGGCCA CCAGCTCTCA 
GACCAGGAAG AAGCTGACCA TGGCCGGGGC TATGAGGGGG ACTCCTCCAG TGGCTCAGGT 
CGATCATGGG GTGAGCGTTC TCGCCGGTCC AGGGCAGGTC GCCGCGGCAG GGTAGGCAGC 
CATGGGCGCC GGAGTTCGGG TGGCGGATCT GAAGCCAACG GTCTGGACCT GGTGTCTGGC 
TATAAGCGCT TGCCGAAGCA GGATGTGCTA ATGAGGCCAC TAAAGTCCGT GCTGGTGAAA 
CGAAGAAACA GCGAAGAGTT TGGGGTGAAG CTAGGCAGTC AGATCTTTAT AAAACACATC 
ACGGAATCTG GCCTTGCTGC TCGGAACCAT GGGCTGCAGG AGGGAGACCT TATCTTACAG 
ATCAACGGGG TGTCCAGTGC TAATCTGTCC CTGAGTGACA CACGAAGGCT CATTGAGAAG 
TCGGAGGGGG AGCTGACGTT GCTGGTGCTA AGAGACAGTG GGCAGTTCCT GGTGAACATC 
CCTCCCGCAG TGAGCGACAG CGACAGCTCC CTAATGGAAG ACATCTCAGA CCTGACCTCA 
GAATTGTCCC AGGCGCCCCC TTCCCACGTT CCTCCGCCAC CTCTGAAGGG TCAGAGGAGC 
CCTGAGGACA GCCAGACAGA CTCCCCAGTG GAGACCCCGC AGCCCCGGAG GCGGGAACGG 
TCAGTGAATT CAAGAGCCAT CGCTGAACCA GAGTCTCCTG GAGAAAGCCG CTATGACATT 
TACAGGGTTC CTAGCCGGCA GAGCCTGGAG GACCGTGGCT ACAGCCCCGA CACTCGTGTC 
GTGAGCTTCC CCAAGGGCGC GAGTATTGGC TTGCGGCTGG CCGGCGGCAA CGACGTGGGC 
ATCTTCGTAT CCGGGGTGCA GGCAGGCAGC CCGGCCGACG GGCAAGGCAT CCAGGAAGGG 
GATGAGATCT TGCAGGTGAA TGGTATGCCA TTTCGGAACC TGACTCGGGA GGAGGCCGTG 
CAGTTTCTGT TGGGGCTGCC TCCAGGGGAA GACATGGAGC TGGTAACACA GAGCAAACAG 
GACATCTTTA AGAAGATGGT CCAGTCTCGC GTGGGTGACT CCTTCTACAT CCGCACACAC 
TTTGAGCTGG AGCCGAGCCC ACCCTATGGC CTGGGCTTCA CCCGCGGTGA TGTCTTCCAC 
GTAGTGGACA CGTTGTACCC GGGCTCCGGG CCAGGCCATG GGCACAGCAG CCATGGGGGC 
CTCTGGCTGG CCGCCCGCAT GGGCAGAGAT CTGCGAGAGC AAGAACGCGG CGTCATCCCC 
AACCAGAGCA GGGCCGAGCA GCTGGCCAGT CTGGAGGCTG CGCAGCGGGC CGCGGGCGTG 
GGGCCAGGCG CCTCAGTGGG TTCCAACCCA CGGGCGGAGT TTTGGCGACT CCGAGGTCTC 
CGCCGAGGGA CCAAGAAGGC GTCGACCCAG CGGAGCCGCG AGGACCTATC AGCGCTGACC 
AGGCAGGGAC ACTACCCGCC CTATGAGCGT GTGGTGCTCA GAGAAGCCAG TTTTAAGCGC 
CCCGTGGTGA TCCTAGGACC AGTGGCAGAC ATTGCCATGA AGAAACTGAC CACAGAGATG 
CCAGAAGAGT TTGAGATTGC AGAAAGCATG TCCCGGACTG ACAGCCCATC CAAGATCATC 
AAACTGGACA CAGTGCGGGT GATCGCAGAG AGGGATAAGC ACGCACTCCT GGATGTCACC 
CCCTCTGCCA TCGAGCGCCT CAACTACGTG CAGTACTACC CCATCGTCAT CTTTTGTGCC 
CCAGAGAGCC GGCCGGCCCT CAAGGCCCTC CGGGAGTGGC TGGCCCCGGC CTCTCGCCGC 
AGCAGCCGCC GCCTGTATGC GCAGGCCCAG AAACTGCAGA AACACAGTGG CCACCTCTTC 
ACCGCCACCA TCCCCCTGCA TGGCACTAGT GACTCCTGGT ACCAGGAAGT CAAGGCTGTA 
ATACAGCAAC AGCAGGCGCG ACCGATCTGG ACCGCGGAGG ACCAGCTGAA CAGCTCATCC 
GAGGACCTTG ACCTGCCGGG CCATGGTCTG GCCGCCAGCT CTGGAGACCT CAGCTGTGAC 
AGTCGGACCA ACAGCGACTA TGAGGACACC GACGGCGAGG GCGCCTATAC GGACAGAGAG 
GGCGGACCCC AGGATGTCGA TGAGGAGGTC GCACCCACAG CCCTGGCCCG GTCCTCAGAG 
CCCGTATGGG TAGATGATCA CCAGGGGCTG ATGGGTCATG GGACAACTAT CACAGACAAA 
TGGGAGACAC AGGCTGACAG CCACTACACC CAGGACCAGC GGCGCCAGGA CAGCATGCGG 
ACCTACAAGC ACGAGGCCCT GAGGAAGAAG TTCACAAGAG CCCGGGATGT GGAATCCTCT 
GATGACGAGG GCTATGACTG GGGCCCAGCC ACCGACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Different effects of ZO-1, ZO-2 and ZO-3 silencing on kidney collecting duct principal cell proliferation and adhesion.
Cell cycle (Georgetown, Tex.)13(19)3059-75(2014)
Qiao X,Roth I,F?raille E,Hasler U

Tight function zonula occludens-3 regulates cyclin D1-dependent cell proliferation.
Molecular biology of the cell22(10)1677-85(2011 May)
Capaldo CT,Koch S,Kwon M,Laur O,Parkos CA,Nusrat A

Roles of ZO-1 and ZO-2 in establishment of the belt-like adherens and tight junctions with paracellular permselective barrier function.
Annals of the New York Academy of Sciences116544-52(2009 May)
Tsukita S,Katsuno T,Yamazaki Y,Umeda K,Tamura A,Tsukita S

Serum response factor is required for sprouting angiogenesis and vascular integrity.
Developmental cell15(3)448-61(2008 Sep)
Franco CA,Mericskay M,Parlakian A,Gary-Bobo G,Gao-Li J,Paulin D,Gustafsson E,Li Z

Identification of transcripts with enriched expression in the developing and adult pancreas.
Genome biology9(6)R99(2008)
Hoffman BG,Zavaglia B,Witzsche J,Ruiz de Algara T,Beach M,Hoodless PA,Jones SJ,Marra MA,Helgason CD

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Tjp3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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