tjp3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tjp3
Entrez Gene ID	100127773
Full Name	tight junction protein 3
Synonyms	zo-3,zo3
General protein information	Preferred Names tight junction protein 3 Names tight junction protein ZO-3 tight junction protein 3 (zona occludens 3)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018096588.2	XP_017952077.1	tight junction protein ZO-3 isoform X2
XM_018096590.1	XP_017952079.1	tight junction protein ZO-3 isoform X3
XM_004911053.4	XP_004911110.1	tight junction protein ZO-3 isoform X1
XM_018096588.1	XP_017952077.1	tight junction protein ZO-3 isoform X3
XM_004911053.3	XP_004911110.1	tight junction protein ZO-3 isoform X1
XM_018096586.1	XP_017952075.1	tight junction protein ZO-3 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TJP3	XP_001135804.2
Mus musculus (house mouse)	Tjp3	NP_001269025.1
Rattus norvegicus (Norway rat)	Tjp3	NP_001101543.1
Xenopus tropicalis (tropical clawed frog)	tjp3	XP_002941439.2
	TJP3	XP_001117962.1
Bos taurus (cattle)	TJP3	NP_001039339.1
Homo sapiens (human)	TJP3	NP_001254490.1
Canis lupus familiaris (dog)	TJP3	NP_001003202.1
Gallus gallus (chicken)	TJP3	XP_003642888.2

Gene Ontology

The following tjp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tjp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04412	XM_004911053.4 Latest version!	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa31053	XM_018096588.2 Latest version!	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa41994	XM_018096586.1 Latest version!	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa31053	XM_018096590.1 Latest version!	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OXa31053	XM_018096588.1	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa04412	XM_004911053.3	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tjp3 ( XM_004911053.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911110.1 Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04412
Clone ID Related Accession (Same CDS sequence)	XM_004911053.4 , XM_004911053.3
Accession Version	XM_004911053.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004911053.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTGC GGTTCCAGGT GTCAGATATG GAGGAAATGA CAATATGGGA ACAGTACACT 
GTGACACTAA TCCGGGACCC TCGCAGAGGT TTTGGTATTG CAATCTCTGG AGGAAGAGAC 
CGTCCATTAG GAACAGATGG AGACAATTCT GTTATTGTTT CAGATGTTAT TCGTGGTGGA 
CCAGCTGATG GTAGACTTCA GACACGAGAT CGCATTGTCA TGGTTAATGG CGTTTCAATG 
GAAAATGTGT CATCCTCATT TGCCATCCAA ACACTAAAAT CATGTGCTAA GACTGCAAAT 
GTGACTGTGA AACGGCCACG AAAGATTCAG ATTCCTGTCA GCAACTCCAT GCATCCAGCT 
TCTACTTATT CCTCCCATAC CCAAAAATCC TCAGACCCTG TCTTCTCAGA TACCTCAAGA 
AGAGAAAAGG ATGATGGGTC AGATTCCCCT TTACCATGGA GAGGGAGATA TGAAAGCTCA 
AATCAGGAAC CTCCTGAGGA CACAGCAAGA GGCTATGAAG GAGATTCATC TAGCGGCCGC 
AGCTCCCAGG GGTCTTTCAG TCACTCATCT CGTCATACCA GACATCGAAG GGGAAGAACT 
GTTGGTAGTG AAGGGGTAGA TTATGCAAAG AGGAAAGTAG GAAGAGAAAC AGTTAGTTCG 
GGCAGTGAGA CAGGAAGACG GTCCAGGAGA AGAGAGAGTG GCAGCGATGT GATTGACTCT 
AGCAGTGAGG CACGTGGATA TTACAGCAAG AGGAAAGAGA CTGGCAGTGA AATGGGGGGG 
TCTGGTAGTG AGATAGGGGA AAGATACCAA AATGGCCTAG AACTGGTGTC TGGGTTCAAG 
AGACTTCCTA TCCAGGATGT GCCAGCCAAA CCTATAAAAG CTGTTCTGGT GAAACGTGCA 
GAAAACCAAG AATATGGTTT AAAGTTGGGT AGTCAGATCT TCATTAAGCA CATAACAGAA 
AGTGGATTGG CAGCTCAAGA GAAGGCATTA CAAGAAGGAG ACCTCATTCT AAAGATTAAT 
GGAGTGACTA GTGAGAATAT GTCATTGGCT GACACTCGGA GACTCATTGA GAAGTCAAGG 
GGCAAACTTA CCCTCACTGT TTTGAGAGAC AATCGACAGT TCCTGGTTAA TATCCCAGAA 
GTGAGAGATA GTGAGAGTGA GGGAAGAAGC TCCCCTCGAG AGGATATCTC TGAAATAGGT 
TCTGAAGATT TTCCACCACC ATCGGAAGCT CCCCCACCCC CACCAGCACA TCAACAGATA 
AGCACGTCTG AGAGTGATGG TAGTGCCTCG GAAGAGCAAG GAATGAATGG ATCAGAAGCT 
GTGCAAAAAA TGGAAAATTC CAAAGTTGAG ATTGCTGTAA AAAATGAAGA AGCACAGGTG 
GTGTCGACAT ACAGAGAGGA ATCAGTGAAT CCCAATAACA ACACGAGAGA AGACTTGAGA 
ACAGGGTACA GTCCGGATGC TCGTGTAATT CAGTTCATGA AAGAAAAGAG TATTGGTCTG 
CGTCTTGCAG GTGGAAATGA CGTGGGAATA TTTGTGGCTG CCGTACAGGC AGCAAGCCCT 
GCAGAACGAG AAGGAATGAA AGAGGGTGAT CAGATCCTGC AGGTGAATGA TACAAGCTTC 
CATAACTTGA CCCGTGAGGA TGCTGTGCAG TATTTAATGG GCCTTCCACA AAACGAGGAT 
GTCATATTCC TTACACAGGG CAAAGAAGAC ATTTACAGAA AAATGATTAA ATCCAATGTT 
GGAGATTCCT TTTATATCCG TACACACTTC GACTATGAGT CAGATGCTCC ATCCGGCCTA 
AGTTTTACTC GAGGAGAAAT ATTTCATATC GTAGACACAA TGTATCGTGG GAAGATAGGA 
AGCTGGCTAG CTGTCCGTGT TGCCAGGGAT TTAAAGGAAA AGGAAAAGGG AATAATACCA 
AACAACAACA GGGCAGAGCA GTATGCCAGT TTGGAACCAG TACTAAAGCC ACAAAGCTCC 
AGTGGACAGA GGGCAGAGTT CTGGAAGTTA AGGGGCCTGC GTGGAGCTAA GAAATTACTA 
AGGAAGAGTA GAGAAGATTT AGCTGCACTG AGCAAGAAGA TTAAATATCC TCCATATGAA 
AGAGTGGTGC TTCGGGAAGC AAGCTTTAAA AGACCTGTAG TTGTTATGGG TCCAATCACA 
GATATTGCTC ATCAGAAGCT TTGTGCAGAT TTGCCCCAAG AGTTTGAGTC TGCAGAGAGT 
GTCACGCGAG ATGGAGGAGG GACCTCTAAG GTCATTAAAC TGGATGTTGT AAGGGAAATT 
GCTGCAAAGA ATAAGCATGC ACTCCTTGAG ATCACTCCAA CAGCTGTAGA ACATCTTAAC 
TACATGCAGT TCTATCCTAT TGTGGTATTT TGTAACCCTG AAAACAGACA AGGAGTAAAA 
GCCATGAGGA AATGGCTGCT TCCTGACTCG AGAAAGAGTT CGAGGCGTTT GTATGCACAA 
GCTGTTAAGC AGCGCAAGAA CAACTTTCAT CTATTTACTG CCACAATAAA TCTGAGTGGA 
GCAAGTGACT CATGGCTTCG CAGCTTGAAA GAAATTATTC AAACTGAGCA AACAAAACCA 
ATATGGACAA CAGAGGATAA GGAGAATACA ATCACAGAGC CACTGGATCT CCTGGATGAC 
TCCACAGCTG AAGTCAATGA CTACCTCAGT TGTGATAGCC GTGCAAACAG TGACTATGAG 
GAAACTGATG CTGAGGGAGG AGCTTATACT GACCAGGAAC TGGAAGAAGA AATTCAAGAG 
CCAGCACTTG CTAGGTCTTC TGAACCAGTG CTGGATTACC AGTCAACCTA TTACAGAGCC 
CATTCGCCAG ATGACTATCC TTCAGAATAC AAAGACCAAT CTTTGCCTGA GACAAACAAC 
ATCCATGGAA CCAAGCCAAG ACCTGAATTA AGTTCTAACT ACAGAAGTTC TTTACCATCT 
AATGATGACA GCCCTCAACA GGAATCCGTA AGCCAGCCAC AAGGCCAATG GAGTGCAAGT 
GCTATGAACA GTCGAGAATA TGAACACGAT GCTCTGCGCA AAAAGTTCAA CAATGCAAGA 
TATTCCAGCT CAGATGAGGA GGAAGATGCT TGGGGTGGGC CTGCAACTGA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911110.1
CDS	70..3186
Translation	MAVRFQVSDMEEMTIWEQYTVTLIRDPRRGFGIAISGGRDRPLGTDGDNSVIVSDVIRGGPADGRLQTRDRIVMVNGVSMENVSSSFAIQTLKSCAKTANVTVKRPRKIQIPVSNSMHPASTYSSHTQKSSDPVFSDTSRREKDDGSDSPLPWRGRYESSNQEPPEDTARGYEGDSSSGRSSQGSFSHSSRHTRHRRGRTVGSEGVDYAKRKVGRETVSSGSETGRRSRRRESGSDVIDSSSEARGYYSKRKETGSEMGGSGSEIGERYQNGLELVSGFKRLPIQDVPAKPIKAVLVKRAENQEYGLKLGSQIFIKHITESGLAAQEKALQEGDLILKINGVTSENMSLADTRRLIEKSRGKLTLTVLRDNRQFLVNIPEVRDSESEGRSSPREDISEIGSEDFPPPSEAPPPPPAHQQISTSESDGSASEEQGMNGSEAVQKMENSKVEIAVKNEEAQVVSTYREESVNPNNNTREDLRTGYSPDARVIQFMKEKSIGLRLAGGNDVGIFVAAVQAASPAEREGMKEGDQILQVNDTSFHNLTREDAVQYLMGLPQNEDVIFLTQGKEDIYRKMIKSNVGDSFYIRTHFDYESDAPSGLSFTRGEIFHIVDTMYRGKIGSWLAVRVARDLKEKEKGIIPNNNRAEQYASLEPVLKPQSSSGQRAEFWKLRGLRGAKKLLRKSREDLAALSKKIKYPPYERVVLREASFKRPVVVMGPITDIAHQKLCADLPQEFESAESVTRDGGGTSKVIKLDVVREIAAKNKHALLEITPTAVEHLNYMQFYPIVVFCNPENRQGVKAMRKWLLPDSRKSSRRLYAQAVKQRKNNFHLFTATINLSGASDSWLRSLKEIIQTEQTKPIWTTEDKENTITEPLDLLDDSTAEVNDYLSCDSRANSDYEETDAEGGAYTDQELEEEIQEPALARSSEPVLDYQSTYYRAHSPDDYPSEYKDQSLPETNNIHGTKPRPELSSNYRSSLPSNDDSPQQESVSQPQGQWSASAMNSREYEHDALRKKFNNARYSSSDEEEDAWGGPATDL

Target ORF information:

RefSeq Version	XM_004911053.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911053.4

ORF Insert Sequence:

ATGGCCGTGC GGTTCCAGGT GTCAGATATG GAGGAAATGA CAATATGGGA ACAGTACACT 
GTGACACTAA TCCGGGACCC TCGCAGAGGT TTTGGTATTG CAATCTCTGG AGGAAGAGAC 
CGTCCATTAG GAACAGATGG AGACAATTCT GTTATTGTTT CAGATGTTAT TCGTGGTGGA 
CCAGCTGATG GTAGACTTCA GACACGAGAT CGCATTGTCA TGGTTAATGG CGTTTCAATG 
GAAAATGTGT CATCCTCATT TGCCATCCAA ACACTAAAAT CATGTGCTAA GACTGCAAAT 
GTGACTGTGA AACGGCCACG AAAGATTCAG ATTCCTGTCA GCAACTCCAT GCATCCAGCT 
TCTACTTATT CCTCCCATAC CCAAAAATCC TCAGACCCTG TCTTCTCAGA TACCTCAAGA 
AGAGAAAAGG ATGATGGGTC AGATTCCCCT TTACCATGGA GAGGGAGATA TGAAAGCTCA 
AATCAGGAAC CTCCTGAGGA CACAGCAAGA GGCTATGAAG GAGATTCATC TAGCGGCCGC 
AGCTCCCAGG GGTCTTTCAG TCACTCATCT CGTCATACCA GACATCGAAG GGGAAGAACT 
GTTGGTAGTG AAGGGGTAGA TTATGCAAAG AGGAAAGTAG GAAGAGAAAC AGTTAGTTCG 
GGCAGTGAGA CAGGAAGACG GTCCAGGAGA AGAGAGAGTG GCAGCGATGT GATTGACTCT 
AGCAGTGAGG CACGTGGATA TTACAGCAAG AGGAAAGAGA CTGGCAGTGA AATGGGGGGG 
TCTGGTAGTG AGATAGGGGA AAGATACCAA AATGGCCTAG AACTGGTGTC TGGGTTCAAG 
AGACTTCCTA TCCAGGATGT GCCAGCCAAA CCTATAAAAG CTGTTCTGGT GAAACGTGCA 
GAAAACCAAG AATATGGTTT AAAGTTGGGT AGTCAGATCT TCATTAAGCA CATAACAGAA 
AGTGGATTGG CAGCTCAAGA GAAGGCATTA CAAGAAGGAG ACCTCATTCT AAAGATTAAT 
GGAGTGACTA GTGAGAATAT GTCATTGGCT GACACTCGGA GACTCATTGA GAAGTCAAGG 
GGCAAACTTA CCCTCACTGT TTTGAGAGAC AATCGACAGT TCCTGGTTAA TATCCCAGAA 
GTGAGAGATA GTGAGAGTGA GGGAAGAAGC TCCCCTCGAG AGGATATCTC TGAAATAGGT 
TCTGAAGATT TTCCACCACC ATCGGAAGCT CCCCCACCCC CACCAGCACA TCAACAGATA 
AGCACGTCTG AGAGTGATGG TAGTGCCTCG GAAGAGCAAG GAATGAATGG ATCAGAAGCT 
GTGCAAAAAA TGGAAAATTC CAAAGTTGAG ATTGCTGTAA AAAATGAAGA AGCACAGGTG 
GTGTCGACAT ACAGAGAGGA ATCAGTGAAT CCCAATAACA ACACGAGAGA AGACTTGAGA 
ACAGGGTACA GTCCGGATGC TCGTGTAATT CAGTTCATGA AAGAAAAGAG TATTGGTCTG 
CGTCTTGCAG GTGGAAATGA CGTGGGAATA TTTGTGGCTG CCGTACAGGC AGCAAGCCCT 
GCAGAACGAG AAGGAATGAA AGAGGGTGAT CAGATCCTGC AGGTGAATGA TACAAGCTTC 
CATAACTTGA CCCGTGAGGA TGCTGTGCAG TATTTAATGG GCCTTCCACA AAACGAGGAT 
GTCATATTCC TTACACAGGG CAAAGAAGAC ATTTACAGAA AAATGATTAA ATCCAATGTT 
GGAGATTCCT TTTATATCCG TACACACTTC GACTATGAGT CAGATGCTCC ATCCGGCCTA 
AGTTTTACTC GAGGAGAAAT ATTTCATATC GTAGACACAA TGTATCGTGG GAAGATAGGA 
AGCTGGCTAG CTGTCCGTGT TGCCAGGGAT TTAAAGGAAA AGGAAAAGGG AATAATACCA 
AACAACAACA GGGCAGAGCA GTATGCCAGT TTGGAACCAG TACTAAAGCC ACAAAGCTCC 
AGTGGACAGA GGGCAGAGTT CTGGAAGTTA AGGGGCCTGC GTGGAGCTAA GAAATTACTA 
AGGAAGAGTA GAGAAGATTT AGCTGCACTG AGCAAGAAGA TTAAATATCC TCCATATGAA 
AGAGTGGTGC TTCGGGAAGC AAGCTTTAAA AGACCTGTAG TTGTTATGGG TCCAATCACA 
GATATTGCTC ATCAGAAGCT TTGTGCAGAT TTGCCCCAAG AGTTTGAGTC TGCAGAGAGT 
GTCACGCGAG ATGGAGGAGG GACCTCTAAG GTCATTAAAC TGGATGTTGT AAGGGAAATT 
GCTGCAAAGA ATAAGCATGC ACTCCTTGAG ATCACTCCAA CAGCTGTAGA ACATCTTAAC 
TACATGCAGT TCTATCCTAT TGTGGTATTT TGTAACCCTG AAAACAGACA AGGAGTAAAA 
GCCATGAGGA AATGGCTGCT TCCTGACTCG AGAAAGAGTT CGAGGCGTTT GTATGCACAA 
GCTGTTAAGC AGCGCAAGAA CAACTTTCAT CTATTTACTG CCACAATAAA TCTGAGTGGA 
GCAAGTGACT CATGGCTTCG CAGCTTGAAA GAAATTATTC AAACTGAGCA AACAAAACCA 
ATATGGACAA CAGAGGATAA GGAGAATACA ATCACAGAGC CACTGGATCT CCTGGATGAC 
TCCACAGCTG AAGTCAATGA CTACCTCAGT TGTGATAGCC GTGCAAACAG TGACTATGAG 
GAAACTGATG CTGAGGGAGG AGCTTATACT GACCAGGAAC TGGAAGAAGA AATTCAAGAG 
CCAGCACTTG CTAGGTCTTC TGAACCAGTG CTGGATTACC AGTCAACCTA TTACAGAGCC 
CATTCGCCAG ATGACTATCC TTCAGAATAC AAAGACCAAT CTTTGCCTGA GACAAACAAC 
ATCCATGGAA CCAAGCCAAG ACCTGAATTA AGTTCTAACT ACAGAAGTTC TTTACCATCT 
AATGATGACA GCCCTCAACA GGAATCCGTA AGCCAGCCAC AAGGCCAATG GAGTGCAAGT 
GCTATGAACA GTCGAGAATA TGAACACGAT GCTCTGCGCA AAAAGTTCAA CAATGCAAGA 
TATTCCAGCT CAGATGAGGA GGAAGATGCT TGGGGTGGGC CTGCAACTGA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp3 ( XM_018096588.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952077.1 Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa31053
Clone ID Related Accession (Same CDS sequence)	XM_018096588.2 , XM_018096590.1 , XM_018096588.1
Accession Version	XM_018096588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA TGACAATATG GGAACAGTAC ACTGTGACAC TAATCCGGGA CCCTCGCAGA 
GGTTTTGGTA TTGCAATCTC TGGAGGAAGA GACCGTCCAT TAGGAACAGA TGGAGACAAT 
TCTGTTATTG TTTCAGATGT TATTCGTGGT GGACCAGCTG ATGGTAGACT TCAGACACGA 
GATCGCATTG TCATGGTTAA TGGCGTTTCA ATGGAAAATG TGTCATCCTC ATTTGCCATC 
CAAACACTAA AATCATGTGC TAAGACTGCA AATGTGACTG TGAAACGGCC ACGAAAGATT 
CAGATTCCTG TCAGCAACTC CATGCATCCA GCTTCTACTT ATTCCTCCCA TACCCAAAAA 
TCCTCAGACC CTGTCTTCTC AGATACCTCA AGAAGAGAAA AGGATGATGG GTCAGATTCC 
CCTTTACCAT GGAGAGGGAG ATATGAAAGC TCAAATCAGG AACCTCCTGA GGACACAGCA 
AGAGGCTATG AAGGAGATTC ATCTAGCGGC CGCAGCTCCC AGGGGTCTTT CAGTCACTCA 
TCTCGTCATA CCAGACATCG AAGGGGAAGA ACTGTTGGTA GTGAAGGGGT AGATTATGCA 
AAGAGGAAAG TAGGAAGAGA AACAGTTAGT TCGGGCAGTG AGACAGGAAG ACGGTCCAGG 
AGAAGAGAGA GTGGCAGCGA TGTGATTGAC TCTAGCAGTG AGGCACGTGG ATATTACAGC 
AAGAGGAAAG AGACTGGCAG TGAAATGGGG GGGTCTGGTA GTGAGATAGG GGAAAGATAC 
CAAAATGGCC TAGAACTGGT GTCTGGGTTC AAGAGACTTC CTATCCAGGA TGTGCCAGCC 
AAACCTATAA AAGCTGTTCT GGTGAAACGT GCAGAAAACC AAGAATATGG TTTAAAGTTG 
GGTAGTCAGA TCTTCATTAA GCACATAACA GAAAGTGGAT TGGCAGCTCA AGAGAAGGCA 
TTACAAGAAG GAGACCTCAT TCTAAAGATT AATGGAGTGA CTAGTGAGAA TATGTCATTG 
GCTGACACTC GGAGACTCAT TGAGAAGTCA AGGGGCAAAC TTACCCTCAC TGTTTTGAGA 
GACAATCGAC AGTTCCTGGT TAATATCCCA GAAGTGAGAG ATAGTGAGAG TGAGGGAAGA 
AGCTCCCCTC GAGAGGATAT CTCTGAAATA GGTTCTGAAG ATTTTCCACC ACCATCGGAA 
GCTCCCCCAC CCCCACCAGC ACATCAACAG ATAAGCACGT CTGAGAGTGA TGGTAGTGCC 
TCGGAAGAGC AAGGAATGAA TGGATCAGAA GCTGTGCAAA AAATGGAAAA TTCCAAAGTT 
GAGATTGCTG TAAAAAATGA AGAAGCACAG GTGGTGTCGA CATACAGAGA GGAATCAGTG 
AATCCCAATA ACAACACGAG AGAAGACTTG AGAACAGGGT ACAGTCCGGA TGCTCGTGTA 
ATTCAGTTCA TGAAAGAAAA GAGTATTGGT CTGCGTCTTG CAGGTGGAAA TGACGTGGGA 
ATATTTGTGG CTGCCGTACA GGCAGCAAGC CCTGCAGAAC GAGAAGGAAT GAAAGAGGGT 
GATCAGATCC TGCAGGTGAA TGATACAAGC TTCCATAACT TGACCCGTGA GGATGCTGTG 
CAGTATTTAA TGGGCCTTCC ACAAAACGAG GATGTCATAT TCCTTACACA GGGCAAAGAA 
GACATTTACA GAAAAATGAT TAAATCCAAT GTTGGAGATT CCTTTTATAT CCGTACACAC 
TTCGACTATG AGTCAGATGC TCCATCCGGC CTAAGTTTTA CTCGAGGAGA AATATTTCAT 
ATCGTAGACA CAATGTATCG TGGGAAGATA GGAAGCTGGC TAGCTGTCCG TGTTGCCAGG 
GATTTAAAGG AAAAGGAAAA GGGAATAATA CCAAACAACA ACAGGGCAGA GCAGTATGCC 
AGTTTGGAAC CAGTACTAAA GCCACAAAGC TCCAGTGGAC AGAGGGCAGA GTTCTGGAAG 
TTAAGGGGCC TGCGTGGAGC TAAGAAATTA CTAAGGAAGA GTAGAGAAGA TTTAGCTGCA 
CTGAGCAAGA AGATTAAATA TCCTCCATAT GAAAGAGTGG TGCTTCGGGA AGCAAGCTTT 
AAAAGACCTG TAGTTGTTAT GGGTCCAATC ACAGATATTG CTCATCAGAA GCTTTGTGCA 
GATTTGCCCC AAGAGTTTGA GTCTGCAGAG AGTGTCACGC GAGATGGAGG AGGGACCTCT 
AAGGTCATTA AACTGGATGT TGTAAGGGAA ATTGCTGCAA AGAATAAGCA TGCACTCCTT 
GAGATCACTC CAACAGCTGT AGAACATCTT AACTACATGC AGTTCTATCC TATTGTGGTA 
TTTTGTAACC CTGAAAACAG ACAAGGAGTA AAAGCCATGA GGAAATGGCT GCTTCCTGAC 
TCGAGAAAGA GTTCGAGGCG TTTGTATGCA CAAGCTGTTA AGCAGCGCAA GAACAACTTT 
CATCTATTTA CTGCCACAAT AAATCTGAGT GGAGCAAGTG ACTCATGGCT TCGCAGCTTG 
AAAGAAATTA TTCAAACTGA GCAAACAAAA CCAATATGGA CAACAGAGGA TAAGGAGAAT 
ACAATCACAG AGCCACTGGA TCTCCTGGAT GACTCCACAG CTGAAGTCAA TGACTACCTC 
AGTTGTGATA GCCGTGCAAA CAGTGACTAT GAGGAAACTG ATGCTGAGGG AGGAGCTTAT 
ACTGACCAGG AACTGGAAGA AGAAATTCAA GAGCCAGCAC TTGCTAGGTC TTCTGAACCA 
GTGCTGGATT ACCAGTCAAC CTATTACAGA GCCCATTCGC CAGATGACTA TCCTTCAGAA 
TACAAAGACC AATCTTTGCC TGAGACAAAC AACATCCATG GAACCAAGCC AAGACCTGAA 
TTAAGTTCTA ACTACAGAAG TTCTTTACCA TCTAATGATG ACAGCCCTCA ACAGGAATCC 
GTAAGCCAGC CACAAGGCCA ATGGAGTGCA AGTGCTATGA ACAGTCGAGA ATATGAACAC 
GATGCTCTGC GCAAAAAGTT CAACAATGCA AGATATTCCA GCTCAGATGA GGAGGAAGAT 
GCTTGGGGTG GGCCTGCAAC TGACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952077.1
CDS	187..3276
Translation	MEEMTIWEQYTVTLIRDPRRGFGIAISGGRDRPLGTDGDNSVIVSDVIRGGPADGRLQTRDRIVMVNGVSMENVSSSFAIQTLKSCAKTANVTVKRPRKIQIPVSNSMHPASTYSSHTQKSSDPVFSDTSRREKDDGSDSPLPWRGRYESSNQEPPEDTARGYEGDSSSGRSSQGSFSHSSRHTRHRRGRTVGSEGVDYAKRKVGRETVSSGSETGRRSRRRESGSDVIDSSSEARGYYSKRKETGSEMGGSGSEIGERYQNGLELVSGFKRLPIQDVPAKPIKAVLVKRAENQEYGLKLGSQIFIKHITESGLAAQEKALQEGDLILKINGVTSENMSLADTRRLIEKSRGKLTLTVLRDNRQFLVNIPEVRDSESEGRSSPREDISEIGSEDFPPPSEAPPPPPAHQQISTSESDGSASEEQGMNGSEAVQKMENSKVEIAVKNEEAQVVSTYREESVNPNNNTREDLRTGYSPDARVIQFMKEKSIGLRLAGGNDVGIFVAAVQAASPAEREGMKEGDQILQVNDTSFHNLTREDAVQYLMGLPQNEDVIFLTQGKEDIYRKMIKSNVGDSFYIRTHFDYESDAPSGLSFTRGEIFHIVDTMYRGKIGSWLAVRVARDLKEKEKGIIPNNNRAEQYASLEPVLKPQSSSGQRAEFWKLRGLRGAKKLLRKSREDLAALSKKIKYPPYERVVLREASFKRPVVVMGPITDIAHQKLCADLPQEFESAESVTRDGGGTSKVIKLDVVREIAAKNKHALLEITPTAVEHLNYMQFYPIVVFCNPENRQGVKAMRKWLLPDSRKSSRRLYAQAVKQRKNNFHLFTATINLSGASDSWLRSLKEIIQTEQTKPIWTTEDKENTITEPLDLLDDSTAEVNDYLSCDSRANSDYEETDAEGGAYTDQELEEEIQEPALARSSEPVLDYQSTYYRAHSPDDYPSEYKDQSLPETNNIHGTKPRPELSSNYRSSLPSNDDSPQQESVSQPQGQWSASAMNSREYEHDALRKKFNNARYSSSDEEEDAWGGPATDL

Target ORF information:

RefSeq Version	XM_018096588.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096588.2

ORF Insert Sequence:

ATGGAGGAAA TGACAATATG GGAACAGTAC ACTGTGACAC TAATCCGGGA CCCTCGCAGA 
GGTTTTGGTA TTGCAATCTC TGGAGGAAGA GACCGTCCAT TAGGAACAGA TGGAGACAAT 
TCTGTTATTG TTTCAGATGT TATTCGTGGT GGACCAGCTG ATGGTAGACT TCAGACACGA 
GATCGCATTG TCATGGTTAA TGGCGTTTCA ATGGAAAATG TGTCATCCTC ATTTGCCATC 
CAAACACTAA AATCATGTGC TAAGACTGCA AATGTGACTG TGAAACGGCC ACGAAAGATT 
CAGATTCCTG TCAGCAACTC CATGCATCCA GCTTCTACTT ATTCCTCCCA TACCCAAAAA 
TCCTCAGACC CTGTCTTCTC AGATACCTCA AGAAGAGAAA AGGATGATGG GTCAGATTCC 
CCTTTACCAT GGAGAGGGAG ATATGAAAGC TCAAATCAGG AACCTCCTGA GGACACAGCA 
AGAGGCTATG AAGGAGATTC ATCTAGCGGC CGCAGCTCCC AGGGGTCTTT CAGTCACTCA 
TCTCGTCATA CCAGACATCG AAGGGGAAGA ACTGTTGGTA GTGAAGGGGT AGATTATGCA 
AAGAGGAAAG TAGGAAGAGA AACAGTTAGT TCGGGCAGTG AGACAGGAAG ACGGTCCAGG 
AGAAGAGAGA GTGGCAGCGA TGTGATTGAC TCTAGCAGTG AGGCACGTGG ATATTACAGC 
AAGAGGAAAG AGACTGGCAG TGAAATGGGG GGGTCTGGTA GTGAGATAGG GGAAAGATAC 
CAAAATGGCC TAGAACTGGT GTCTGGGTTC AAGAGACTTC CTATCCAGGA TGTGCCAGCC 
AAACCTATAA AAGCTGTTCT GGTGAAACGT GCAGAAAACC AAGAATATGG TTTAAAGTTG 
GGTAGTCAGA TCTTCATTAA GCACATAACA GAAAGTGGAT TGGCAGCTCA AGAGAAGGCA 
TTACAAGAAG GAGACCTCAT TCTAAAGATT AATGGAGTGA CTAGTGAGAA TATGTCATTG 
GCTGACACTC GGAGACTCAT TGAGAAGTCA AGGGGCAAAC TTACCCTCAC TGTTTTGAGA 
GACAATCGAC AGTTCCTGGT TAATATCCCA GAAGTGAGAG ATAGTGAGAG TGAGGGAAGA 
AGCTCCCCTC GAGAGGATAT CTCTGAAATA GGTTCTGAAG ATTTTCCACC ACCATCGGAA 
GCTCCCCCAC CCCCACCAGC ACATCAACAG ATAAGCACGT CTGAGAGTGA TGGTAGTGCC 
TCGGAAGAGC AAGGAATGAA TGGATCAGAA GCTGTGCAAA AAATGGAAAA TTCCAAAGTT 
GAGATTGCTG TAAAAAATGA AGAAGCACAG GTGGTGTCGA CATACAGAGA GGAATCAGTG 
AATCCCAATA ACAACACGAG AGAAGACTTG AGAACAGGGT ACAGTCCGGA TGCTCGTGTA 
ATTCAGTTCA TGAAAGAAAA GAGTATTGGT CTGCGTCTTG CAGGTGGAAA TGACGTGGGA 
ATATTTGTGG CTGCCGTACA GGCAGCAAGC CCTGCAGAAC GAGAAGGAAT GAAAGAGGGT 
GATCAGATCC TGCAGGTGAA TGATACAAGC TTCCATAACT TGACCCGTGA GGATGCTGTG 
CAGTATTTAA TGGGCCTTCC ACAAAACGAG GATGTCATAT TCCTTACACA GGGCAAAGAA 
GACATTTACA GAAAAATGAT TAAATCCAAT GTTGGAGATT CCTTTTATAT CCGTACACAC 
TTCGACTATG AGTCAGATGC TCCATCCGGC CTAAGTTTTA CTCGAGGAGA AATATTTCAT 
ATCGTAGACA CAATGTATCG TGGGAAGATA GGAAGCTGGC TAGCTGTCCG TGTTGCCAGG 
GATTTAAAGG AAAAGGAAAA GGGAATAATA CCAAACAACA ACAGGGCAGA GCAGTATGCC 
AGTTTGGAAC CAGTACTAAA GCCACAAAGC TCCAGTGGAC AGAGGGCAGA GTTCTGGAAG 
TTAAGGGGCC TGCGTGGAGC TAAGAAATTA CTAAGGAAGA GTAGAGAAGA TTTAGCTGCA 
CTGAGCAAGA AGATTAAATA TCCTCCATAT GAAAGAGTGG TGCTTCGGGA AGCAAGCTTT 
AAAAGACCTG TAGTTGTTAT GGGTCCAATC ACAGATATTG CTCATCAGAA GCTTTGTGCA 
GATTTGCCCC AAGAGTTTGA GTCTGCAGAG AGTGTCACGC GAGATGGAGG AGGGACCTCT 
AAGGTCATTA AACTGGATGT TGTAAGGGAA ATTGCTGCAA AGAATAAGCA TGCACTCCTT 
GAGATCACTC CAACAGCTGT AGAACATCTT AACTACATGC AGTTCTATCC TATTGTGGTA 
TTTTGTAACC CTGAAAACAG ACAAGGAGTA AAAGCCATGA GGAAATGGCT GCTTCCTGAC 
TCGAGAAAGA GTTCGAGGCG TTTGTATGCA CAAGCTGTTA AGCAGCGCAA GAACAACTTT 
CATCTATTTA CTGCCACAAT AAATCTGAGT GGAGCAAGTG ACTCATGGCT TCGCAGCTTG 
AAAGAAATTA TTCAAACTGA GCAAACAAAA CCAATATGGA CAACAGAGGA TAAGGAGAAT 
ACAATCACAG AGCCACTGGA TCTCCTGGAT GACTCCACAG CTGAAGTCAA TGACTACCTC 
AGTTGTGATA GCCGTGCAAA CAGTGACTAT GAGGAAACTG ATGCTGAGGG AGGAGCTTAT 
ACTGACCAGG AACTGGAAGA AGAAATTCAA GAGCCAGCAC TTGCTAGGTC TTCTGAACCA 
GTGCTGGATT ACCAGTCAAC CTATTACAGA GCCCATTCGC CAGATGACTA TCCTTCAGAA 
TACAAAGACC AATCTTTGCC TGAGACAAAC AACATCCATG GAACCAAGCC AAGACCTGAA 
TTAAGTTCTA ACTACAGAAG TTCTTTACCA TCTAATGATG ACAGCCCTCA ACAGGAATCC 
GTAAGCCAGC CACAAGGCCA ATGGAGTGCA AGTGCTATGA ACAGTCGAGA ATATGAACAC 
GATGCTCTGC GCAAAAAGTT CAACAATGCA AGATATTCCA GCTCAGATGA GGAGGAAGAT 
GCTTGGGGTG GGCCTGCAAC TGACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp3 ( XM_018096586.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952075.1 Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa41994
Clone ID Related Accession (Same CDS sequence)	XM_018096586.1
Accession Version	XM_018096586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTAC TGGATAGTGT GTCAGATATG GAGGAAATGA CAATATGGGA ACAGTACACT 
GTGACACTAA TCCGGGACCC TCGCAGAGGT TTTGGTATTG CAATCTCTGG AGGAAGAGAC 
CGTCCATTAG GAACAGATGG AGACAATTCT GTTATTGTTT CAGATGTTAT TCGTGGTGGA 
CCAGCTGATG GTAGACTTCA GACACGAGAT CGCATTGTCA TGGTTAATGG CGTTTCAATG 
GAAAATGTGT CATCCTCATT TGCCATCCAA ACACTAAAAT CATGTGCTAA GACTGCAAAT 
GTGACTGTGA AACGGCCACG AAAGATTCAG ATTCCTGTCA GCAACTCCAT GCATCCAGCT 
TCTACTTATT CCTCCCATAC CCAAAAATCC TCAGACCCTG TCTTCTCAGA TACCTCAAGA 
AGAGAAAAGG ATGATGGGTC AGATTCCCCT TTACCATGGA GAGGGAGATA TGAAAGCTCA 
AATCAGGAAC CTCCTGAGGA CACAGCAAGA GGCTATGAAG GAGATTCATC TAGCGGCCGC 
AGCTCCCAGG GGTCTTTCAG TCACTCATCT CGTCATACCA GACATCGAAG GGGAAGAACT 
GTTGGTAGTG AAGGGGTAGA TTATGCAAAG AGGAAAGTAG GAAGAGAAAC AGTTAGTTCG 
GGCAGTGAGA CAGGAAGACG GTCCAGGAGA AGAGAGAGTG GCAGCGATGT GATTGACTCT 
AGCAGTGAGG CACGTGGATA TTACAGCAAG AGGAAAGAGA CTGGCAGTGA AATGGGGGGG 
TCTGGTAGTG AGATAGGGGA AAGATACCAA AATGGCCTAG AACTGGTGTC TGGGTTCAAG 
AGACTTCCTA TCCAGGATGT GCCAGCCAAA CCTATAAAAG CTGTTCTGGT GAAACGTGCA 
GAAAACCAAG AATATGGTTT AAAGTTGGGT AGTCAGATCT TCATTAAGCA CATAACAGAA 
AGTGGATTGG CAGCTCAAGA GAAGGCATTA CAAGAAGGAG ACCTCATTCT AAAGATTAAT 
GGAGTGACTA GTGAGAATAT GTCATTGGCT GACACTCGGA GACTCATTGA GAAGTCAAGG 
GGCAAACTTA CCCTCACTGT TTTGAGAGAC AATCGACAGT TCCTGGTTAA TATCCCAGAA 
GTGAGAGATA GTGAGAGTGA GGGAAGAAGC TCCCCTCGAG AGGATATCTC TGAAATAGGT 
TCTGAAGATT TTCCACCACC ATCGGAAGCT CCCCCACCCC CACCAGCACA TCAACAGATA 
AGCACGTCTG AGAGTGATGG TAGTGCCTCG GAAGAGCAAG GAATGAATGG ATCAGAAGCT 
GTGCAAAAAA TGGAAAATTC CAAAGTTGAG ATTGCTGTAA AAAATGAAGA AGCACAGGTG 
GTGTCGACAT ACAGAGAGGA ATCAGTGAAT CCCAATAACA ACACGAGAGA AGACTTGAGA 
ACAGGGTACA GTCCGGATGC TCGTGTAATT CAGTTCATGA AAGAAAAGAG TATTGGTCTG 
CGTCTTGCAG GTGGAAATGA CGTGGGAATA TTTGTGGCTG CCGTACAGGC AGCAAGCCCT 
GCAGAACGAG AAGGAATGAA AGAGGGTGAT CAGATCCTGC AGGTGAATGA TACAAGCTTC 
CATAACTTGA CCCGTGAGGA TGCTGTGCAG TATTTAATGG GCCTTCCACA AAACGAGGAT 
GTCATATTCC TTACACAGGG CAAAGAAGAC ATTTACAGAA AAATGATTAA ATCCAATGTT 
GGAGATTCCT TTTATATCCG TACACACTTC GACTATGAGT CAGATGCTCC ATCCGGCCTA 
AGTTTTACTC GAGGAGAAAT ATTTCATATC GTAGACACAA TGTATCGTGG GAAGATAGGA 
AGCTGGCTAG CTGTCCGTGT TGCCAGGGAT TTAAAGGAAA AGGAAAAGGG AATAATACCA 
AACAACAACA GGGCAGAGCA GTATGCCAGT TTGGAACCAG TACTAAAGCC ACAAAGCTCC 
AGTGGACAGA GGGCAGAGTT CTGGAAGTTA AGGGGCCTGC GTGGAGCTAA GAAATTACTA 
AGGAAGAGTA GAGAAGATTT AGCTGCACTG AGCAAGAAGA TTAAATATCC TCCATATGAA 
AGAGTGGTGC TTCGGGAAGC AAGCTTTAAA AGACCTGTAG TTGTTATGGG TCCAATCACA 
GATATTGCTC ATCAGAAGCT TTGTGCAGAT TTGCCCCAAG AGTTTGAGTC TGCAGAGAGT 
GTCACGCGAG ATGGAGGAGG GACCTCTAAG GTCATTAAAC TGGATGTTGT AAGGGAAATT 
GCTGCAAAGA ATAAGCATGC ACTCCTTGAG ATCACTCCAA CAGCTGTAGA ACATCTTAAC 
TACATGCAGT TCTATCCTAT TGTGGTATTT TGTAACCCTG AAAACAGACA AGGAGTAAAA 
GCCATGAGGA AATGGCTGCT TCCTGACTCG AGAAAGAGTT CGAGGCGTTT GTATGCACAA 
GCTGTTAAGC AGCGCAAGAA CAACTTTCAT CTATTTACTG CCACAATAAA TCTGAGTGGA 
GCAAGTGACT CATGGCTTCG CAGCTTGAAA GAAATTATTC AAACTGAGCA AACAAAACCA 
ATATGGACAA CAGAGGATAA GGAGAATACA ATCACAGAGC CACTGGATCT CCTGGATGAC 
TCCACAGCTG AAGTCAATGA CTACCTCAGT TGTGATAGCC GTGCAAACAG TGACTATGAG 
GAAACTGATG CTGAGGGAGG AGCTTATACT GACCAGGAAC TGGAAGAAGA AATTCAAGAG 
CCAGCACTTG CTAGGTCTTC TGAACCAGTG CTGGATTACC AGTCAACCTA TTACAGAGCC 
CATTCGCCAG ATGACTATCC TTCAGAATAC AAAGACCAAT CTTTGCCTGA GACAAACAAC 
ATCCATGGAA CCAAGCCAAG ACCTGAATTA AGTTCTAACT ACAGAAGTTC TTTACCATCT 
AATGATGACA GCCCTCAACA GGAATCCGTA AGCCAGCCAC AAGGCCAATG GAGTGCAAGT 
GCTATGAACA GTCGAGAATA TGAACACGAT GCTCTGCGCA AAAAGTTCAA CAATGCAAGA 
TATTCCAGCT CAGATGAGGA GGAAGATGCT TGGGGTGGGC CTGCAACTGA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952075.1
CDS	107..3223
Translation	MNVLDSVSDMEEMTIWEQYTVTLIRDPRRGFGIAISGGRDRPLGTDGDNSVIVSDVIRGGPADGRLQTRDRIVMVNGVSMENVSSSFAIQTLKSCAKTANVTVKRPRKIQIPVSNSMHPASTYSSHTQKSSDPVFSDTSRREKDDGSDSPLPWRGRYESSNQEPPEDTARGYEGDSSSGRSSQGSFSHSSRHTRHRRGRTVGSEGVDYAKRKVGRETVSSGSETGRRSRRRESGSDVIDSSSEARGYYSKRKETGSEMGGSGSEIGERYQNGLELVSGFKRLPIQDVPAKPIKAVLVKRAENQEYGLKLGSQIFIKHITESGLAAQEKALQEGDLILKINGVTSENMSLADTRRLIEKSRGKLTLTVLRDNRQFLVNIPEVRDSESEGRSSPREDISEIGSEDFPPPSEAPPPPPAHQQISTSESDGSASEEQGMNGSEAVQKMENSKVEIAVKNEEAQVVSTYREESVNPNNNTREDLRTGYSPDARVIQFMKEKSIGLRLAGGNDVGIFVAAVQAASPAEREGMKEGDQILQVNDTSFHNLTREDAVQYLMGLPQNEDVIFLTQGKEDIYRKMIKSNVGDSFYIRTHFDYESDAPSGLSFTRGEIFHIVDTMYRGKIGSWLAVRVARDLKEKEKGIIPNNNRAEQYASLEPVLKPQSSSGQRAEFWKLRGLRGAKKLLRKSREDLAALSKKIKYPPYERVVLREASFKRPVVVMGPITDIAHQKLCADLPQEFESAESVTRDGGGTSKVIKLDVVREIAAKNKHALLEITPTAVEHLNYMQFYPIVVFCNPENRQGVKAMRKWLLPDSRKSSRRLYAQAVKQRKNNFHLFTATINLSGASDSWLRSLKEIIQTEQTKPIWTTEDKENTITEPLDLLDDSTAEVNDYLSCDSRANSDYEETDAEGGAYTDQELEEEIQEPALARSSEPVLDYQSTYYRAHSPDDYPSEYKDQSLPETNNIHGTKPRPELSSNYRSSLPSNDDSPQQESVSQPQGQWSASAMNSREYEHDALRKKFNNARYSSSDEEEDAWGGPATDL

Target ORF information:

RefSeq Version	XM_018096586.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096586.1

ORF Insert Sequence:

ATGAATGTAC TGGATAGTGT GTCAGATATG GAGGAAATGA CAATATGGGA ACAGTACACT 
GTGACACTAA TCCGGGACCC TCGCAGAGGT TTTGGTATTG CAATCTCTGG AGGAAGAGAC 
CGTCCATTAG GAACAGATGG AGACAATTCT GTTATTGTTT CAGATGTTAT TCGTGGTGGA 
CCAGCTGATG GTAGACTTCA GACACGAGAT CGCATTGTCA TGGTTAATGG CGTTTCAATG 
GAAAATGTGT CATCCTCATT TGCCATCCAA ACACTAAAAT CATGTGCTAA GACTGCAAAT 
GTGACTGTGA AACGGCCACG AAAGATTCAG ATTCCTGTCA GCAACTCCAT GCATCCAGCT 
TCTACTTATT CCTCCCATAC CCAAAAATCC TCAGACCCTG TCTTCTCAGA TACCTCAAGA 
AGAGAAAAGG ATGATGGGTC AGATTCCCCT TTACCATGGA GAGGGAGATA TGAAAGCTCA 
AATCAGGAAC CTCCTGAGGA CACAGCAAGA GGCTATGAAG GAGATTCATC TAGCGGCCGC 
AGCTCCCAGG GGTCTTTCAG TCACTCATCT CGTCATACCA GACATCGAAG GGGAAGAACT 
GTTGGTAGTG AAGGGGTAGA TTATGCAAAG AGGAAAGTAG GAAGAGAAAC AGTTAGTTCG 
GGCAGTGAGA CAGGAAGACG GTCCAGGAGA AGAGAGAGTG GCAGCGATGT GATTGACTCT 
AGCAGTGAGG CACGTGGATA TTACAGCAAG AGGAAAGAGA CTGGCAGTGA AATGGGGGGG 
TCTGGTAGTG AGATAGGGGA AAGATACCAA AATGGCCTAG AACTGGTGTC TGGGTTCAAG 
AGACTTCCTA TCCAGGATGT GCCAGCCAAA CCTATAAAAG CTGTTCTGGT GAAACGTGCA 
GAAAACCAAG AATATGGTTT AAAGTTGGGT AGTCAGATCT TCATTAAGCA CATAACAGAA 
AGTGGATTGG CAGCTCAAGA GAAGGCATTA CAAGAAGGAG ACCTCATTCT AAAGATTAAT 
GGAGTGACTA GTGAGAATAT GTCATTGGCT GACACTCGGA GACTCATTGA GAAGTCAAGG 
GGCAAACTTA CCCTCACTGT TTTGAGAGAC AATCGACAGT TCCTGGTTAA TATCCCAGAA 
GTGAGAGATA GTGAGAGTGA GGGAAGAAGC TCCCCTCGAG AGGATATCTC TGAAATAGGT 
TCTGAAGATT TTCCACCACC ATCGGAAGCT CCCCCACCCC CACCAGCACA TCAACAGATA 
AGCACGTCTG AGAGTGATGG TAGTGCCTCG GAAGAGCAAG GAATGAATGG ATCAGAAGCT 
GTGCAAAAAA TGGAAAATTC CAAAGTTGAG ATTGCTGTAA AAAATGAAGA AGCACAGGTG 
GTGTCGACAT ACAGAGAGGA ATCAGTGAAT CCCAATAACA ACACGAGAGA AGACTTGAGA 
ACAGGGTACA GTCCGGATGC TCGTGTAATT CAGTTCATGA AAGAAAAGAG TATTGGTCTG 
CGTCTTGCAG GTGGAAATGA CGTGGGAATA TTTGTGGCTG CCGTACAGGC AGCAAGCCCT 
GCAGAACGAG AAGGAATGAA AGAGGGTGAT CAGATCCTGC AGGTGAATGA TACAAGCTTC 
CATAACTTGA CCCGTGAGGA TGCTGTGCAG TATTTAATGG GCCTTCCACA AAACGAGGAT 
GTCATATTCC TTACACAGGG CAAAGAAGAC ATTTACAGAA AAATGATTAA ATCCAATGTT 
GGAGATTCCT TTTATATCCG TACACACTTC GACTATGAGT CAGATGCTCC ATCCGGCCTA 
AGTTTTACTC GAGGAGAAAT ATTTCATATC GTAGACACAA TGTATCGTGG GAAGATAGGA 
AGCTGGCTAG CTGTCCGTGT TGCCAGGGAT TTAAAGGAAA AGGAAAAGGG AATAATACCA 
AACAACAACA GGGCAGAGCA GTATGCCAGT TTGGAACCAG TACTAAAGCC ACAAAGCTCC 
AGTGGACAGA GGGCAGAGTT CTGGAAGTTA AGGGGCCTGC GTGGAGCTAA GAAATTACTA 
AGGAAGAGTA GAGAAGATTT AGCTGCACTG AGCAAGAAGA TTAAATATCC TCCATATGAA 
AGAGTGGTGC TTCGGGAAGC AAGCTTTAAA AGACCTGTAG TTGTTATGGG TCCAATCACA 
GATATTGCTC ATCAGAAGCT TTGTGCAGAT TTGCCCCAAG AGTTTGAGTC TGCAGAGAGT 
GTCACGCGAG ATGGAGGAGG GACCTCTAAG GTCATTAAAC TGGATGTTGT AAGGGAAATT 
GCTGCAAAGA ATAAGCATGC ACTCCTTGAG ATCACTCCAA CAGCTGTAGA ACATCTTAAC 
TACATGCAGT TCTATCCTAT TGTGGTATTT TGTAACCCTG AAAACAGACA AGGAGTAAAA 
GCCATGAGGA AATGGCTGCT TCCTGACTCG AGAAAGAGTT CGAGGCGTTT GTATGCACAA 
GCTGTTAAGC AGCGCAAGAA CAACTTTCAT CTATTTACTG CCACAATAAA TCTGAGTGGA 
GCAAGTGACT CATGGCTTCG CAGCTTGAAA GAAATTATTC AAACTGAGCA AACAAAACCA 
ATATGGACAA CAGAGGATAA GGAGAATACA ATCACAGAGC CACTGGATCT CCTGGATGAC 
TCCACAGCTG AAGTCAATGA CTACCTCAGT TGTGATAGCC GTGCAAACAG TGACTATGAG 
GAAACTGATG CTGAGGGAGG AGCTTATACT GACCAGGAAC TGGAAGAAGA AATTCAAGAG 
CCAGCACTTG CTAGGTCTTC TGAACCAGTG CTGGATTACC AGTCAACCTA TTACAGAGCC 
CATTCGCCAG ATGACTATCC TTCAGAATAC AAAGACCAAT CTTTGCCTGA GACAAACAAC 
ATCCATGGAA CCAAGCCAAG ACCTGAATTA AGTTCTAACT ACAGAAGTTC TTTACCATCT 
AATGATGACA GCCCTCAACA GGAATCCGTA AGCCAGCCAC AAGGCCAATG GAGTGCAAGT 
GCTATGAACA GTCGAGAATA TGAACACGAT GCTCTGCGCA AAAAGTTCAA CAATGCAAGA 
TATTCCAGCT CAGATGAGGA GGAAGATGCT TGGGGTGGGC CTGCAACTGA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp3 ( XM_004911053.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004911110.1 Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa04412
Clone ID Related Accession (Same CDS sequence)	XM_004911053.4 , XM_004911053.3
Accession Version	XM_004911053.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004911053.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTGC GGTTCCAGGT GTCAGATATG GAGGAAATGA CAATATGGGA ACAGTACACT 
GTGACACTAA TCCGGGACCC TCGCAGAGGT TTTGGTATTG CAATCTCTGG AGGAAGAGAC 
CGTCCATTAG GAACAGATGG AGACAATTCT GTTATTGTTT CAGATGTTAT TCGTGGTGGA 
CCAGCTGATG GTAGACTTCA GACACGAGAT CGCATTGTCA TGGTTAATGG CGTTTCAATG 
GAAAATGTGT CATCCTCATT TGCCATCCAA ACACTAAAAT CATGTGCTAA GACTGCAAAT 
GTGACTGTGA AACGGCCACG AAAGATTCAG ATTCCTGTCA GCAACTCCAT GCATCCAGCT 
TCTACTTATT CCTCCCATAC CCAAAAATCC TCAGACCCTG TCTTCTCAGA TACCTCAAGA 
AGAGAAAAGG ATGATGGGTC AGATTCCCCT TTACCATGGA GAGGGAGATA TGAAAGCTCA 
AATCAGGAAC CTCCTGAGGA CACAGCAAGA GGCTATGAAG GAGATTCATC TAGCGGCCGC 
AGCTCCCAGG GGTCTTTCAG TCACTCATCT CGTCATACCA GACATCGAAG GGGAAGAACT 
GTTGGTAGTG AAGGGGTAGA TTATGCAAAG AGGAAAGTAG GAAGAGAAAC AGTTAGTTCG 
GGCAGTGAGA CAGGAAGACG GTCCAGGAGA AGAGAGAGTG GCAGCGATGT GATTGACTCT 
AGCAGTGAGG CACGTGGATA TTACAGCAAG AGGAAAGAGA CTGGCAGTGA AATGGGGGGG 
TCTGGTAGTG AGATAGGGGA AAGATACCAA AATGGCCTAG AACTGGTGTC TGGGTTCAAG 
AGACTTCCTA TCCAGGATGT GCCAGCCAAA CCTATAAAAG CTGTTCTGGT GAAACGTGCA 
GAAAACCAAG AATATGGTTT AAAGTTGGGT AGTCAGATCT TCATTAAGCA CATAACAGAA 
AGTGGATTGG CAGCTCAAGA GAAGGCATTA CAAGAAGGAG ACCTCATTCT AAAGATTAAT 
GGAGTGACTA GTGAGAATAT GTCATTGGCT GACACTCGGA GACTCATTGA GAAGTCAAGG 
GGCAAACTTA CCCTCACTGT TTTGAGAGAC AATCGACAGT TCCTGGTTAA TATCCCAGAA 
GTGAGAGATA GTGAGAGTGA GGGAAGAAGC TCCCCTCGAG AGGATATCTC TGAAATAGGT 
TCTGAAGATT TTCCACCACC ATCGGAAGCT CCCCCACCCC CACCAGCACA TCAACAGATA 
AGCACGTCTG AGAGTGATGG TAGTGCCTCG GAAGAGCAAG GAATGAATGG ATCAGAAGCT 
GTGCAAAAAA TGGAAAATTC CAAAGTTGAG ATTGCTGTAA AAAATGAAGA AGCACAGGTG 
GTGTCGACAT ACAGAGAGGA ATCAGTGAAT CCCAATAACA ACACGAGAGA AGACTTGAGA 
ACAGGGTACA GTCCGGATGC TCGTGTAATT CAGTTCATGA AAGAAAAGAG TATTGGTCTG 
CGTCTTGCAG GTGGAAATGA CGTGGGAATA TTTGTGGCTG CCGTACAGGC AGCAAGCCCT 
GCAGAACGAG AAGGAATGAA AGAGGGTGAT CAGATCCTGC AGGTGAATGA TACAAGCTTC 
CATAACTTGA CCCGTGAGGA TGCTGTGCAG TATTTAATGG GCCTTCCACA AAACGAGGAT 
GTCATATTCC TTACACAGGG CAAAGAAGAC ATTTACAGAA AAATGATTAA ATCCAATGTT 
GGAGATTCCT TTTATATCCG TACACACTTC GACTATGAGT CAGATGCTCC ATCCGGCCTA 
AGTTTTACTC GAGGAGAAAT ATTTCATATC GTAGACACAA TGTATCGTGG GAAGATAGGA 
AGCTGGCTAG CTGTCCGTGT TGCCAGGGAT TTAAAGGAAA AGGAAAAGGG AATAATACCA 
AACAACAACA GGGCAGAGCA GTATGCCAGT TTGGAACCAG TACTAAAGCC ACAAAGCTCC 
AGTGGACAGA GGGCAGAGTT CTGGAAGTTA AGGGGCCTGC GTGGAGCTAA GAAATTACTA 
AGGAAGAGTA GAGAAGATTT AGCTGCACTG AGCAAGAAGA TTAAATATCC TCCATATGAA 
AGAGTGGTGC TTCGGGAAGC AAGCTTTAAA AGACCTGTAG TTGTTATGGG TCCAATCACA 
GATATTGCTC ATCAGAAGCT TTGTGCAGAT TTGCCCCAAG AGTTTGAGTC TGCAGAGAGT 
GTCACGCGAG ATGGAGGAGG GACCTCTAAG GTCATTAAAC TGGATGTTGT AAGGGAAATT 
GCTGCAAAGA ATAAGCATGC ACTCCTTGAG ATCACTCCAA CAGCTGTAGA ACATCTTAAC 
TACATGCAGT TCTATCCTAT TGTGGTATTT TGTAACCCTG AAAACAGACA AGGAGTAAAA 
GCCATGAGGA AATGGCTGCT TCCTGACTCG AGAAAGAGTT CGAGGCGTTT GTATGCACAA 
GCTGTTAAGC AGCGCAAGAA CAACTTTCAT CTATTTACTG CCACAATAAA TCTGAGTGGA 
GCAAGTGACT CATGGCTTCG CAGCTTGAAA GAAATTATTC AAACTGAGCA AACAAAACCA 
ATATGGACAA CAGAGGATAA GGAGAATACA ATCACAGAGC CACTGGATCT CCTGGATGAC 
TCCACAGCTG AAGTCAATGA CTACCTCAGT TGTGATAGCC GTGCAAACAG TGACTATGAG 
GAAACTGATG CTGAGGGAGG AGCTTATACT GACCAGGAAC TGGAAGAAGA AATTCAAGAG 
CCAGCACTTG CTAGGTCTTC TGAACCAGTG CTGGATTACC AGTCAACCTA TTACAGAGCC 
CATTCGCCAG ATGACTATCC TTCAGAATAC AAAGACCAAT CTTTGCCTGA GACAAACAAC 
ATCCATGGAA CCAAGCCAAG ACCTGAATTA AGTTCTAACT ACAGAAGTTC TTTACCATCT 
AATGATGACA GCCCTCAACA GGAATCCGTA AGCCAGCCAC AAGGCCAATG GAGTGCAAGT 
GCTATGAACA GTCGAGAATA TGAACACGAT GCTCTGCGCA AAAAGTTCAA CAATGCAAGA 
TATTCCAGCT CAGATGAGGA GGAAGATGCT TGGGGTGGGC CTGCAACTGA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004911110.1
CDS	74..3190
Translation	MAVRFQVSDMEEMTIWEQYTVTLIRDPRRGFGIAISGGRDRPLGTDGDNSVIVSDVIRGGPADGRLQTRDRIVMVNGVSMENVSSSFAIQTLKSCAKTANVTVKRPRKIQIPVSNSMHPASTYSSHTQKSSDPVFSDTSRREKDDGSDSPLPWRGRYESSNQEPPEDTARGYEGDSSSGRSSQGSFSHSSRHTRHRRGRTVGSEGVDYAKRKVGRETVSSGSETGRRSRRRESGSDVIDSSSEARGYYSKRKETGSEMGGSGSEIGERYQNGLELVSGFKRLPIQDVPAKPIKAVLVKRAENQEYGLKLGSQIFIKHITESGLAAQEKALQEGDLILKINGVTSENMSLADTRRLIEKSRGKLTLTVLRDNRQFLVNIPEVRDSESEGRSSPREDISEIGSEDFPPPSEAPPPPPAHQQISTSESDGSASEEQGMNGSEAVQKMENSKVEIAVKNEEAQVVSTYREESVNPNNNTREDLRTGYSPDARVIQFMKEKSIGLRLAGGNDVGIFVAAVQAASPAEREGMKEGDQILQVNDTSFHNLTREDAVQYLMGLPQNEDVIFLTQGKEDIYRKMIKSNVGDSFYIRTHFDYESDAPSGLSFTRGEIFHIVDTMYRGKIGSWLAVRVARDLKEKEKGIIPNNNRAEQYASLEPVLKPQSSSGQRAEFWKLRGLRGAKKLLRKSREDLAALSKKIKYPPYERVVLREASFKRPVVVMGPITDIAHQKLCADLPQEFESAESVTRDGGGTSKVIKLDVVREIAAKNKHALLEITPTAVEHLNYMQFYPIVVFCNPENRQGVKAMRKWLLPDSRKSSRRLYAQAVKQRKNNFHLFTATINLSGASDSWLRSLKEIIQTEQTKPIWTTEDKENTITEPLDLLDDSTAEVNDYLSCDSRANSDYEETDAEGGAYTDQELEEEIQEPALARSSEPVLDYQSTYYRAHSPDDYPSEYKDQSLPETNNIHGTKPRPELSSNYRSSLPSNDDSPQQESVSQPQGQWSASAMNSREYEHDALRKKFNNARYSSSDEEEDAWGGPATDL

Target ORF information:

RefSeq Version	XM_004911053.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004911053.3

ORF Insert Sequence:

ATGGCCGTGC GGTTCCAGGT GTCAGATATG GAGGAAATGA CAATATGGGA ACAGTACACT 
GTGACACTAA TCCGGGACCC TCGCAGAGGT TTTGGTATTG CAATCTCTGG AGGAAGAGAC 
CGTCCATTAG GAACAGATGG AGACAATTCT GTTATTGTTT CAGATGTTAT TCGTGGTGGA 
CCAGCTGATG GTAGACTTCA GACACGAGAT CGCATTGTCA TGGTTAATGG CGTTTCAATG 
GAAAATGTGT CATCCTCATT TGCCATCCAA ACACTAAAAT CATGTGCTAA GACTGCAAAT 
GTGACTGTGA AACGGCCACG AAAGATTCAG ATTCCTGTCA GCAACTCCAT GCATCCAGCT 
TCTACTTATT CCTCCCATAC CCAAAAATCC TCAGACCCTG TCTTCTCAGA TACCTCAAGA 
AGAGAAAAGG ATGATGGGTC AGATTCCCCT TTACCATGGA GAGGGAGATA TGAAAGCTCA 
AATCAGGAAC CTCCTGAGGA CACAGCAAGA GGCTATGAAG GAGATTCATC TAGCGGCCGC 
AGCTCCCAGG GGTCTTTCAG TCACTCATCT CGTCATACCA GACATCGAAG GGGAAGAACT 
GTTGGTAGTG AAGGGGTAGA TTATGCAAAG AGGAAAGTAG GAAGAGAAAC AGTTAGTTCG 
GGCAGTGAGA CAGGAAGACG GTCCAGGAGA AGAGAGAGTG GCAGCGATGT GATTGACTCT 
AGCAGTGAGG CACGTGGATA TTACAGCAAG AGGAAAGAGA CTGGCAGTGA AATGGGGGGG 
TCTGGTAGTG AGATAGGGGA AAGATACCAA AATGGCCTAG AACTGGTGTC TGGGTTCAAG 
AGACTTCCTA TCCAGGATGT GCCAGCCAAA CCTATAAAAG CTGTTCTGGT GAAACGTGCA 
GAAAACCAAG AATATGGTTT AAAGTTGGGT AGTCAGATCT TCATTAAGCA CATAACAGAA 
AGTGGATTGG CAGCTCAAGA GAAGGCATTA CAAGAAGGAG ACCTCATTCT AAAGATTAAT 
GGAGTGACTA GTGAGAATAT GTCATTGGCT GACACTCGGA GACTCATTGA GAAGTCAAGG 
GGCAAACTTA CCCTCACTGT TTTGAGAGAC AATCGACAGT TCCTGGTTAA TATCCCAGAA 
GTGAGAGATA GTGAGAGTGA GGGAAGAAGC TCCCCTCGAG AGGATATCTC TGAAATAGGT 
TCTGAAGATT TTCCACCACC ATCGGAAGCT CCCCCACCCC CACCAGCACA TCAACAGATA 
AGCACGTCTG AGAGTGATGG TAGTGCCTCG GAAGAGCAAG GAATGAATGG ATCAGAAGCT 
GTGCAAAAAA TGGAAAATTC CAAAGTTGAG ATTGCTGTAA AAAATGAAGA AGCACAGGTG 
GTGTCGACAT ACAGAGAGGA ATCAGTGAAT CCCAATAACA ACACGAGAGA AGACTTGAGA 
ACAGGGTACA GTCCGGATGC TCGTGTAATT CAGTTCATGA AAGAAAAGAG TATTGGTCTG 
CGTCTTGCAG GTGGAAATGA CGTGGGAATA TTTGTGGCTG CCGTACAGGC AGCAAGCCCT 
GCAGAACGAG AAGGAATGAA AGAGGGTGAT CAGATCCTGC AGGTGAATGA TACAAGCTTC 
CATAACTTGA CCCGTGAGGA TGCTGTGCAG TATTTAATGG GCCTTCCACA AAACGAGGAT 
GTCATATTCC TTACACAGGG CAAAGAAGAC ATTTACAGAA AAATGATTAA ATCCAATGTT 
GGAGATTCCT TTTATATCCG TACACACTTC GACTATGAGT CAGATGCTCC ATCCGGCCTA 
AGTTTTACTC GAGGAGAAAT ATTTCATATC GTAGACACAA TGTATCGTGG GAAGATAGGA 
AGCTGGCTAG CTGTCCGTGT TGCCAGGGAT TTAAAGGAAA AGGAAAAGGG AATAATACCA 
AACAACAACA GGGCAGAGCA GTATGCCAGT TTGGAACCAG TACTAAAGCC ACAAAGCTCC 
AGTGGACAGA GGGCAGAGTT CTGGAAGTTA AGGGGCCTGC GTGGAGCTAA GAAATTACTA 
AGGAAGAGTA GAGAAGATTT AGCTGCACTG AGCAAGAAGA TTAAATATCC TCCATATGAA 
AGAGTGGTGC TTCGGGAAGC AAGCTTTAAA AGACCTGTAG TTGTTATGGG TCCAATCACA 
GATATTGCTC ATCAGAAGCT TTGTGCAGAT TTGCCCCAAG AGTTTGAGTC TGCAGAGAGT 
GTCACGCGAG ATGGAGGAGG GACCTCTAAG GTCATTAAAC TGGATGTTGT AAGGGAAATT 
GCTGCAAAGA ATAAGCATGC ACTCCTTGAG ATCACTCCAA CAGCTGTAGA ACATCTTAAC 
TACATGCAGT TCTATCCTAT TGTGGTATTT TGTAACCCTG AAAACAGACA AGGAGTAAAA 
GCCATGAGGA AATGGCTGCT TCCTGACTCG AGAAAGAGTT CGAGGCGTTT GTATGCACAA 
GCTGTTAAGC AGCGCAAGAA CAACTTTCAT CTATTTACTG CCACAATAAA TCTGAGTGGA 
GCAAGTGACT CATGGCTTCG CAGCTTGAAA GAAATTATTC AAACTGAGCA AACAAAACCA 
ATATGGACAA CAGAGGATAA GGAGAATACA ATCACAGAGC CACTGGATCT CCTGGATGAC 
TCCACAGCTG AAGTCAATGA CTACCTCAGT TGTGATAGCC GTGCAAACAG TGACTATGAG 
GAAACTGATG CTGAGGGAGG AGCTTATACT GACCAGGAAC TGGAAGAAGA AATTCAAGAG 
CCAGCACTTG CTAGGTCTTC TGAACCAGTG CTGGATTACC AGTCAACCTA TTACAGAGCC 
CATTCGCCAG ATGACTATCC TTCAGAATAC AAAGACCAAT CTTTGCCTGA GACAAACAAC 
ATCCATGGAA CCAAGCCAAG ACCTGAATTA AGTTCTAACT ACAGAAGTTC TTTACCATCT 
AATGATGACA GCCCTCAACA GGAATCCGTA AGCCAGCCAC AAGGCCAATG GAGTGCAAGT 
GCTATGAACA GTCGAGAATA TGAACACGAT GCTCTGCGCA AAAAGTTCAA CAATGCAAGA 
TATTCCAGCT CAGATGAGGA GGAAGATGCT TGGGGTGGGC CTGCAACTGA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp3 ( XM_018096590.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952079.1 Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa31053
Clone ID Related Accession (Same CDS sequence)	XM_018096588.2 , XM_018096590.1 , XM_018096588.1
Accession Version	XM_018096590.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA TGACAATATG GGAACAGTAC ACTGTGACAC TAATCCGGGA CCCTCGCAGA 
GGTTTTGGTA TTGCAATCTC TGGAGGAAGA GACCGTCCAT TAGGAACAGA TGGAGACAAT 
TCTGTTATTG TTTCAGATGT TATTCGTGGT GGACCAGCTG ATGGTAGACT TCAGACACGA 
GATCGCATTG TCATGGTTAA TGGCGTTTCA ATGGAAAATG TGTCATCCTC ATTTGCCATC 
CAAACACTAA AATCATGTGC TAAGACTGCA AATGTGACTG TGAAACGGCC ACGAAAGATT 
CAGATTCCTG TCAGCAACTC CATGCATCCA GCTTCTACTT ATTCCTCCCA TACCCAAAAA 
TCCTCAGACC CTGTCTTCTC AGATACCTCA AGAAGAGAAA AGGATGATGG GTCAGATTCC 
CCTTTACCAT GGAGAGGGAG ATATGAAAGC TCAAATCAGG AACCTCCTGA GGACACAGCA 
AGAGGCTATG AAGGAGATTC ATCTAGCGGC CGCAGCTCCC AGGGGTCTTT CAGTCACTCA 
TCTCGTCATA CCAGACATCG AAGGGGAAGA ACTGTTGGTA GTGAAGGGGT AGATTATGCA 
AAGAGGAAAG TAGGAAGAGA AACAGTTAGT TCGGGCAGTG AGACAGGAAG ACGGTCCAGG 
AGAAGAGAGA GTGGCAGCGA TGTGATTGAC TCTAGCAGTG AGGCACGTGG ATATTACAGC 
AAGAGGAAAG AGACTGGCAG TGAAATGGGG GGGTCTGGTA GTGAGATAGG GGAAAGATAC 
CAAAATGGCC TAGAACTGGT GTCTGGGTTC AAGAGACTTC CTATCCAGGA TGTGCCAGCC 
AAACCTATAA AAGCTGTTCT GGTGAAACGT GCAGAAAACC AAGAATATGG TTTAAAGTTG 
GGTAGTCAGA TCTTCATTAA GCACATAACA GAAAGTGGAT TGGCAGCTCA AGAGAAGGCA 
TTACAAGAAG GAGACCTCAT TCTAAAGATT AATGGAGTGA CTAGTGAGAA TATGTCATTG 
GCTGACACTC GGAGACTCAT TGAGAAGTCA AGGGGCAAAC TTACCCTCAC TGTTTTGAGA 
GACAATCGAC AGTTCCTGGT TAATATCCCA GAAGTGAGAG ATAGTGAGAG TGAGGGAAGA 
AGCTCCCCTC GAGAGGATAT CTCTGAAATA GGTTCTGAAG ATTTTCCACC ACCATCGGAA 
GCTCCCCCAC CCCCACCAGC ACATCAACAG ATAAGCACGT CTGAGAGTGA TGGTAGTGCC 
TCGGAAGAGC AAGGAATGAA TGGATCAGAA GCTGTGCAAA AAATGGAAAA TTCCAAAGTT 
GAGATTGCTG TAAAAAATGA AGAAGCACAG GTGGTGTCGA CATACAGAGA GGAATCAGTG 
AATCCCAATA ACAACACGAG AGAAGACTTG AGAACAGGGT ACAGTCCGGA TGCTCGTGTA 
ATTCAGTTCA TGAAAGAAAA GAGTATTGGT CTGCGTCTTG CAGGTGGAAA TGACGTGGGA 
ATATTTGTGG CTGCCGTACA GGCAGCAAGC CCTGCAGAAC GAGAAGGAAT GAAAGAGGGT 
GATCAGATCC TGCAGGTGAA TGATACAAGC TTCCATAACT TGACCCGTGA GGATGCTGTG 
CAGTATTTAA TGGGCCTTCC ACAAAACGAG GATGTCATAT TCCTTACACA GGGCAAAGAA 
GACATTTACA GAAAAATGAT TAAATCCAAT GTTGGAGATT CCTTTTATAT CCGTACACAC 
TTCGACTATG AGTCAGATGC TCCATCCGGC CTAAGTTTTA CTCGAGGAGA AATATTTCAT 
ATCGTAGACA CAATGTATCG TGGGAAGATA GGAAGCTGGC TAGCTGTCCG TGTTGCCAGG 
GATTTAAAGG AAAAGGAAAA GGGAATAATA CCAAACAACA ACAGGGCAGA GCAGTATGCC 
AGTTTGGAAC CAGTACTAAA GCCACAAAGC TCCAGTGGAC AGAGGGCAGA GTTCTGGAAG 
TTAAGGGGCC TGCGTGGAGC TAAGAAATTA CTAAGGAAGA GTAGAGAAGA TTTAGCTGCA 
CTGAGCAAGA AGATTAAATA TCCTCCATAT GAAAGAGTGG TGCTTCGGGA AGCAAGCTTT 
AAAAGACCTG TAGTTGTTAT GGGTCCAATC ACAGATATTG CTCATCAGAA GCTTTGTGCA 
GATTTGCCCC AAGAGTTTGA GTCTGCAGAG AGTGTCACGC GAGATGGAGG AGGGACCTCT 
AAGGTCATTA AACTGGATGT TGTAAGGGAA ATTGCTGCAA AGAATAAGCA TGCACTCCTT 
GAGATCACTC CAACAGCTGT AGAACATCTT AACTACATGC AGTTCTATCC TATTGTGGTA 
TTTTGTAACC CTGAAAACAG ACAAGGAGTA AAAGCCATGA GGAAATGGCT GCTTCCTGAC 
TCGAGAAAGA GTTCGAGGCG TTTGTATGCA CAAGCTGTTA AGCAGCGCAA GAACAACTTT 
CATCTATTTA CTGCCACAAT AAATCTGAGT GGAGCAAGTG ACTCATGGCT TCGCAGCTTG 
AAAGAAATTA TTCAAACTGA GCAAACAAAA CCAATATGGA CAACAGAGGA TAAGGAGAAT 
ACAATCACAG AGCCACTGGA TCTCCTGGAT GACTCCACAG CTGAAGTCAA TGACTACCTC 
AGTTGTGATA GCCGTGCAAA CAGTGACTAT GAGGAAACTG ATGCTGAGGG AGGAGCTTAT 
ACTGACCAGG AACTGGAAGA AGAAATTCAA GAGCCAGCAC TTGCTAGGTC TTCTGAACCA 
GTGCTGGATT ACCAGTCAAC CTATTACAGA GCCCATTCGC CAGATGACTA TCCTTCAGAA 
TACAAAGACC AATCTTTGCC TGAGACAAAC AACATCCATG GAACCAAGCC AAGACCTGAA 
TTAAGTTCTA ACTACAGAAG TTCTTTACCA TCTAATGATG ACAGCCCTCA ACAGGAATCC 
GTAAGCCAGC CACAAGGCCA ATGGAGTGCA AGTGCTATGA ACAGTCGAGA ATATGAACAC 
GATGCTCTGC GCAAAAAGTT CAACAATGCA AGATATTCCA GCTCAGATGA GGAGGAAGAT 
GCTTGGGGTG GGCCTGCAAC TGACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952079.1
CDS	60..3149
Translation	MEEMTIWEQYTVTLIRDPRRGFGIAISGGRDRPLGTDGDNSVIVSDVIRGGPADGRLQTRDRIVMVNGVSMENVSSSFAIQTLKSCAKTANVTVKRPRKIQIPVSNSMHPASTYSSHTQKSSDPVFSDTSRREKDDGSDSPLPWRGRYESSNQEPPEDTARGYEGDSSSGRSSQGSFSHSSRHTRHRRGRTVGSEGVDYAKRKVGRETVSSGSETGRRSRRRESGSDVIDSSSEARGYYSKRKETGSEMGGSGSEIGERYQNGLELVSGFKRLPIQDVPAKPIKAVLVKRAENQEYGLKLGSQIFIKHITESGLAAQEKALQEGDLILKINGVTSENMSLADTRRLIEKSRGKLTLTVLRDNRQFLVNIPEVRDSESEGRSSPREDISEIGSEDFPPPSEAPPPPPAHQQISTSESDGSASEEQGMNGSEAVQKMENSKVEIAVKNEEAQVVSTYREESVNPNNNTREDLRTGYSPDARVIQFMKEKSIGLRLAGGNDVGIFVAAVQAASPAEREGMKEGDQILQVNDTSFHNLTREDAVQYLMGLPQNEDVIFLTQGKEDIYRKMIKSNVGDSFYIRTHFDYESDAPSGLSFTRGEIFHIVDTMYRGKIGSWLAVRVARDLKEKEKGIIPNNNRAEQYASLEPVLKPQSSSGQRAEFWKLRGLRGAKKLLRKSREDLAALSKKIKYPPYERVVLREASFKRPVVVMGPITDIAHQKLCADLPQEFESAESVTRDGGGTSKVIKLDVVREIAAKNKHALLEITPTAVEHLNYMQFYPIVVFCNPENRQGVKAMRKWLLPDSRKSSRRLYAQAVKQRKNNFHLFTATINLSGASDSWLRSLKEIIQTEQTKPIWTTEDKENTITEPLDLLDDSTAEVNDYLSCDSRANSDYEETDAEGGAYTDQELEEEIQEPALARSSEPVLDYQSTYYRAHSPDDYPSEYKDQSLPETNNIHGTKPRPELSSNYRSSLPSNDDSPQQESVSQPQGQWSASAMNSREYEHDALRKKFNNARYSSSDEEEDAWGGPATDL

Target ORF information:

RefSeq Version	XM_018096590.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096590.1

ORF Insert Sequence:

ATGGAGGAAA TGACAATATG GGAACAGTAC ACTGTGACAC TAATCCGGGA CCCTCGCAGA 
GGTTTTGGTA TTGCAATCTC TGGAGGAAGA GACCGTCCAT TAGGAACAGA TGGAGACAAT 
TCTGTTATTG TTTCAGATGT TATTCGTGGT GGACCAGCTG ATGGTAGACT TCAGACACGA 
GATCGCATTG TCATGGTTAA TGGCGTTTCA ATGGAAAATG TGTCATCCTC ATTTGCCATC 
CAAACACTAA AATCATGTGC TAAGACTGCA AATGTGACTG TGAAACGGCC ACGAAAGATT 
CAGATTCCTG TCAGCAACTC CATGCATCCA GCTTCTACTT ATTCCTCCCA TACCCAAAAA 
TCCTCAGACC CTGTCTTCTC AGATACCTCA AGAAGAGAAA AGGATGATGG GTCAGATTCC 
CCTTTACCAT GGAGAGGGAG ATATGAAAGC TCAAATCAGG AACCTCCTGA GGACACAGCA 
AGAGGCTATG AAGGAGATTC ATCTAGCGGC CGCAGCTCCC AGGGGTCTTT CAGTCACTCA 
TCTCGTCATA CCAGACATCG AAGGGGAAGA ACTGTTGGTA GTGAAGGGGT AGATTATGCA 
AAGAGGAAAG TAGGAAGAGA AACAGTTAGT TCGGGCAGTG AGACAGGAAG ACGGTCCAGG 
AGAAGAGAGA GTGGCAGCGA TGTGATTGAC TCTAGCAGTG AGGCACGTGG ATATTACAGC 
AAGAGGAAAG AGACTGGCAG TGAAATGGGG GGGTCTGGTA GTGAGATAGG GGAAAGATAC 
CAAAATGGCC TAGAACTGGT GTCTGGGTTC AAGAGACTTC CTATCCAGGA TGTGCCAGCC 
AAACCTATAA AAGCTGTTCT GGTGAAACGT GCAGAAAACC AAGAATATGG TTTAAAGTTG 
GGTAGTCAGA TCTTCATTAA GCACATAACA GAAAGTGGAT TGGCAGCTCA AGAGAAGGCA 
TTACAAGAAG GAGACCTCAT TCTAAAGATT AATGGAGTGA CTAGTGAGAA TATGTCATTG 
GCTGACACTC GGAGACTCAT TGAGAAGTCA AGGGGCAAAC TTACCCTCAC TGTTTTGAGA 
GACAATCGAC AGTTCCTGGT TAATATCCCA GAAGTGAGAG ATAGTGAGAG TGAGGGAAGA 
AGCTCCCCTC GAGAGGATAT CTCTGAAATA GGTTCTGAAG ATTTTCCACC ACCATCGGAA 
GCTCCCCCAC CCCCACCAGC ACATCAACAG ATAAGCACGT CTGAGAGTGA TGGTAGTGCC 
TCGGAAGAGC AAGGAATGAA TGGATCAGAA GCTGTGCAAA AAATGGAAAA TTCCAAAGTT 
GAGATTGCTG TAAAAAATGA AGAAGCACAG GTGGTGTCGA CATACAGAGA GGAATCAGTG 
AATCCCAATA ACAACACGAG AGAAGACTTG AGAACAGGGT ACAGTCCGGA TGCTCGTGTA 
ATTCAGTTCA TGAAAGAAAA GAGTATTGGT CTGCGTCTTG CAGGTGGAAA TGACGTGGGA 
ATATTTGTGG CTGCCGTACA GGCAGCAAGC CCTGCAGAAC GAGAAGGAAT GAAAGAGGGT 
GATCAGATCC TGCAGGTGAA TGATACAAGC TTCCATAACT TGACCCGTGA GGATGCTGTG 
CAGTATTTAA TGGGCCTTCC ACAAAACGAG GATGTCATAT TCCTTACACA GGGCAAAGAA 
GACATTTACA GAAAAATGAT TAAATCCAAT GTTGGAGATT CCTTTTATAT CCGTACACAC 
TTCGACTATG AGTCAGATGC TCCATCCGGC CTAAGTTTTA CTCGAGGAGA AATATTTCAT 
ATCGTAGACA CAATGTATCG TGGGAAGATA GGAAGCTGGC TAGCTGTCCG TGTTGCCAGG 
GATTTAAAGG AAAAGGAAAA GGGAATAATA CCAAACAACA ACAGGGCAGA GCAGTATGCC 
AGTTTGGAAC CAGTACTAAA GCCACAAAGC TCCAGTGGAC AGAGGGCAGA GTTCTGGAAG 
TTAAGGGGCC TGCGTGGAGC TAAGAAATTA CTAAGGAAGA GTAGAGAAGA TTTAGCTGCA 
CTGAGCAAGA AGATTAAATA TCCTCCATAT GAAAGAGTGG TGCTTCGGGA AGCAAGCTTT 
AAAAGACCTG TAGTTGTTAT GGGTCCAATC ACAGATATTG CTCATCAGAA GCTTTGTGCA 
GATTTGCCCC AAGAGTTTGA GTCTGCAGAG AGTGTCACGC GAGATGGAGG AGGGACCTCT 
AAGGTCATTA AACTGGATGT TGTAAGGGAA ATTGCTGCAA AGAATAAGCA TGCACTCCTT 
GAGATCACTC CAACAGCTGT AGAACATCTT AACTACATGC AGTTCTATCC TATTGTGGTA 
TTTTGTAACC CTGAAAACAG ACAAGGAGTA AAAGCCATGA GGAAATGGCT GCTTCCTGAC 
TCGAGAAAGA GTTCGAGGCG TTTGTATGCA CAAGCTGTTA AGCAGCGCAA GAACAACTTT 
CATCTATTTA CTGCCACAAT AAATCTGAGT GGAGCAAGTG ACTCATGGCT TCGCAGCTTG 
AAAGAAATTA TTCAAACTGA GCAAACAAAA CCAATATGGA CAACAGAGGA TAAGGAGAAT 
ACAATCACAG AGCCACTGGA TCTCCTGGAT GACTCCACAG CTGAAGTCAA TGACTACCTC 
AGTTGTGATA GCCGTGCAAA CAGTGACTAT GAGGAAACTG ATGCTGAGGG AGGAGCTTAT 
ACTGACCAGG AACTGGAAGA AGAAATTCAA GAGCCAGCAC TTGCTAGGTC TTCTGAACCA 
GTGCTGGATT ACCAGTCAAC CTATTACAGA GCCCATTCGC CAGATGACTA TCCTTCAGAA 
TACAAAGACC AATCTTTGCC TGAGACAAAC AACATCCATG GAACCAAGCC AAGACCTGAA 
TTAAGTTCTA ACTACAGAAG TTCTTTACCA TCTAATGATG ACAGCCCTCA ACAGGAATCC 
GTAAGCCAGC CACAAGGCCA ATGGAGTGCA AGTGCTATGA ACAGTCGAGA ATATGAACAC 
GATGCTCTGC GCAAAAAGTT CAACAATGCA AGATATTCCA GCTCAGATGA GGAGGAAGAT 
GCTTGGGGTG GGCCTGCAAC TGACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjp3 ( XM_018096588.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952077.1 Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa31053
Clone ID Related Accession (Same CDS sequence)	XM_018096588.2 , XM_018096590.1 , XM_018096588.1
Accession Version	XM_018096588.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	tight junction protein ZO-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAA TGACAATATG GGAACAGTAC ACTGTGACAC TAATCCGGGA CCCTCGCAGA 
GGTTTTGGTA TTGCAATCTC TGGAGGAAGA GACCGTCCAT TAGGAACAGA TGGAGACAAT 
TCTGTTATTG TTTCAGATGT TATTCGTGGT GGACCAGCTG ATGGTAGACT TCAGACACGA 
GATCGCATTG TCATGGTTAA TGGCGTTTCA ATGGAAAATG TGTCATCCTC ATTTGCCATC 
CAAACACTAA AATCATGTGC TAAGACTGCA AATGTGACTG TGAAACGGCC ACGAAAGATT 
CAGATTCCTG TCAGCAACTC CATGCATCCA GCTTCTACTT ATTCCTCCCA TACCCAAAAA 
TCCTCAGACC CTGTCTTCTC AGATACCTCA AGAAGAGAAA AGGATGATGG GTCAGATTCC 
CCTTTACCAT GGAGAGGGAG ATATGAAAGC TCAAATCAGG AACCTCCTGA GGACACAGCA 
AGAGGCTATG AAGGAGATTC ATCTAGCGGC CGCAGCTCCC AGGGGTCTTT CAGTCACTCA 
TCTCGTCATA CCAGACATCG AAGGGGAAGA ACTGTTGGTA GTGAAGGGGT AGATTATGCA 
AAGAGGAAAG TAGGAAGAGA AACAGTTAGT TCGGGCAGTG AGACAGGAAG ACGGTCCAGG 
AGAAGAGAGA GTGGCAGCGA TGTGATTGAC TCTAGCAGTG AGGCACGTGG ATATTACAGC 
AAGAGGAAAG AGACTGGCAG TGAAATGGGG GGGTCTGGTA GTGAGATAGG GGAAAGATAC 
CAAAATGGCC TAGAACTGGT GTCTGGGTTC AAGAGACTTC CTATCCAGGA TGTGCCAGCC 
AAACCTATAA AAGCTGTTCT GGTGAAACGT GCAGAAAACC AAGAATATGG TTTAAAGTTG 
GGTAGTCAGA TCTTCATTAA GCACATAACA GAAAGTGGAT TGGCAGCTCA AGAGAAGGCA 
TTACAAGAAG GAGACCTCAT TCTAAAGATT AATGGAGTGA CTAGTGAGAA TATGTCATTG 
GCTGACACTC GGAGACTCAT TGAGAAGTCA AGGGGCAAAC TTACCCTCAC TGTTTTGAGA 
GACAATCGAC AGTTCCTGGT TAATATCCCA GAAGTGAGAG ATAGTGAGAG TGAGGGAAGA 
AGCTCCCCTC GAGAGGATAT CTCTGAAATA GGTTCTGAAG ATTTTCCACC ACCATCGGAA 
GCTCCCCCAC CCCCACCAGC ACATCAACAG ATAAGCACGT CTGAGAGTGA TGGTAGTGCC 
TCGGAAGAGC AAGGAATGAA TGGATCAGAA GCTGTGCAAA AAATGGAAAA TTCCAAAGTT 
GAGATTGCTG TAAAAAATGA AGAAGCACAG GTGGTGTCGA CATACAGAGA GGAATCAGTG 
AATCCCAATA ACAACACGAG AGAAGACTTG AGAACAGGGT ACAGTCCGGA TGCTCGTGTA 
ATTCAGTTCA TGAAAGAAAA GAGTATTGGT CTGCGTCTTG CAGGTGGAAA TGACGTGGGA 
ATATTTGTGG CTGCCGTACA GGCAGCAAGC CCTGCAGAAC GAGAAGGAAT GAAAGAGGGT 
GATCAGATCC TGCAGGTGAA TGATACAAGC TTCCATAACT TGACCCGTGA GGATGCTGTG 
CAGTATTTAA TGGGCCTTCC ACAAAACGAG GATGTCATAT TCCTTACACA GGGCAAAGAA 
GACATTTACA GAAAAATGAT TAAATCCAAT GTTGGAGATT CCTTTTATAT CCGTACACAC 
TTCGACTATG AGTCAGATGC TCCATCCGGC CTAAGTTTTA CTCGAGGAGA AATATTTCAT 
ATCGTAGACA CAATGTATCG TGGGAAGATA GGAAGCTGGC TAGCTGTCCG TGTTGCCAGG 
GATTTAAAGG AAAAGGAAAA GGGAATAATA CCAAACAACA ACAGGGCAGA GCAGTATGCC 
AGTTTGGAAC CAGTACTAAA GCCACAAAGC TCCAGTGGAC AGAGGGCAGA GTTCTGGAAG 
TTAAGGGGCC TGCGTGGAGC TAAGAAATTA CTAAGGAAGA GTAGAGAAGA TTTAGCTGCA 
CTGAGCAAGA AGATTAAATA TCCTCCATAT GAAAGAGTGG TGCTTCGGGA AGCAAGCTTT 
AAAAGACCTG TAGTTGTTAT GGGTCCAATC ACAGATATTG CTCATCAGAA GCTTTGTGCA 
GATTTGCCCC AAGAGTTTGA GTCTGCAGAG AGTGTCACGC GAGATGGAGG AGGGACCTCT 
AAGGTCATTA AACTGGATGT TGTAAGGGAA ATTGCTGCAA AGAATAAGCA TGCACTCCTT 
GAGATCACTC CAACAGCTGT AGAACATCTT AACTACATGC AGTTCTATCC TATTGTGGTA 
TTTTGTAACC CTGAAAACAG ACAAGGAGTA AAAGCCATGA GGAAATGGCT GCTTCCTGAC 
TCGAGAAAGA GTTCGAGGCG TTTGTATGCA CAAGCTGTTA AGCAGCGCAA GAACAACTTT 
CATCTATTTA CTGCCACAAT AAATCTGAGT GGAGCAAGTG ACTCATGGCT TCGCAGCTTG 
AAAGAAATTA TTCAAACTGA GCAAACAAAA CCAATATGGA CAACAGAGGA TAAGGAGAAT 
ACAATCACAG AGCCACTGGA TCTCCTGGAT GACTCCACAG CTGAAGTCAA TGACTACCTC 
AGTTGTGATA GCCGTGCAAA CAGTGACTAT GAGGAAACTG ATGCTGAGGG AGGAGCTTAT 
ACTGACCAGG AACTGGAAGA AGAAATTCAA GAGCCAGCAC TTGCTAGGTC TTCTGAACCA 
GTGCTGGATT ACCAGTCAAC CTATTACAGA GCCCATTCGC CAGATGACTA TCCTTCAGAA 
TACAAAGACC AATCTTTGCC TGAGACAAAC AACATCCATG GAACCAAGCC AAGACCTGAA 
TTAAGTTCTA ACTACAGAAG TTCTTTACCA TCTAATGATG ACAGCCCTCA ACAGGAATCC 
GTAAGCCAGC CACAAGGCCA ATGGAGTGCA AGTGCTATGA ACAGTCGAGA ATATGAACAC 
GATGCTCTGC GCAAAAAGTT CAACAATGCA AGATATTCCA GCTCAGATGA GGAGGAAGAT 
GCTTGGGGTG GGCCTGCAAC TGACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952077.1
CDS	189..3278
Translation	MEEMTIWEQYTVTLIRDPRRGFGIAISGGRDRPLGTDGDNSVIVSDVIRGGPADGRLQTRDRIVMVNGVSMENVSSSFAIQTLKSCAKTANVTVKRPRKIQIPVSNSMHPASTYSSHTQKSSDPVFSDTSRREKDDGSDSPLPWRGRYESSNQEPPEDTARGYEGDSSSGRSSQGSFSHSSRHTRHRRGRTVGSEGVDYAKRKVGRETVSSGSETGRRSRRRESGSDVIDSSSEARGYYSKRKETGSEMGGSGSEIGERYQNGLELVSGFKRLPIQDVPAKPIKAVLVKRAENQEYGLKLGSQIFIKHITESGLAAQEKALQEGDLILKINGVTSENMSLADTRRLIEKSRGKLTLTVLRDNRQFLVNIPEVRDSESEGRSSPREDISEIGSEDFPPPSEAPPPPPAHQQISTSESDGSASEEQGMNGSEAVQKMENSKVEIAVKNEEAQVVSTYREESVNPNNNTREDLRTGYSPDARVIQFMKEKSIGLRLAGGNDVGIFVAAVQAASPAEREGMKEGDQILQVNDTSFHNLTREDAVQYLMGLPQNEDVIFLTQGKEDIYRKMIKSNVGDSFYIRTHFDYESDAPSGLSFTRGEIFHIVDTMYRGKIGSWLAVRVARDLKEKEKGIIPNNNRAEQYASLEPVLKPQSSSGQRAEFWKLRGLRGAKKLLRKSREDLAALSKKIKYPPYERVVLREASFKRPVVVMGPITDIAHQKLCADLPQEFESAESVTRDGGGTSKVIKLDVVREIAAKNKHALLEITPTAVEHLNYMQFYPIVVFCNPENRQGVKAMRKWLLPDSRKSSRRLYAQAVKQRKNNFHLFTATINLSGASDSWLRSLKEIIQTEQTKPIWTTEDKENTITEPLDLLDDSTAEVNDYLSCDSRANSDYEETDAEGGAYTDQELEEEIQEPALARSSEPVLDYQSTYYRAHSPDDYPSEYKDQSLPETNNIHGTKPRPELSSNYRSSLPSNDDSPQQESVSQPQGQWSASAMNSREYEHDALRKKFNNARYSSSDEEEDAWGGPATDL

Target ORF information:

RefSeq Version	XM_018096588.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis tight junction protein 3 (tjp3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096588.1

ORF Insert Sequence:

ATGGAGGAAA TGACAATATG GGAACAGTAC ACTGTGACAC TAATCCGGGA CCCTCGCAGA 
GGTTTTGGTA TTGCAATCTC TGGAGGAAGA GACCGTCCAT TAGGAACAGA TGGAGACAAT 
TCTGTTATTG TTTCAGATGT TATTCGTGGT GGACCAGCTG ATGGTAGACT TCAGACACGA 
GATCGCATTG TCATGGTTAA TGGCGTTTCA ATGGAAAATG TGTCATCCTC ATTTGCCATC 
CAAACACTAA AATCATGTGC TAAGACTGCA AATGTGACTG TGAAACGGCC ACGAAAGATT 
CAGATTCCTG TCAGCAACTC CATGCATCCA GCTTCTACTT ATTCCTCCCA TACCCAAAAA 
TCCTCAGACC CTGTCTTCTC AGATACCTCA AGAAGAGAAA AGGATGATGG GTCAGATTCC 
CCTTTACCAT GGAGAGGGAG ATATGAAAGC TCAAATCAGG AACCTCCTGA GGACACAGCA 
AGAGGCTATG AAGGAGATTC ATCTAGCGGC CGCAGCTCCC AGGGGTCTTT CAGTCACTCA 
TCTCGTCATA CCAGACATCG AAGGGGAAGA ACTGTTGGTA GTGAAGGGGT AGATTATGCA 
AAGAGGAAAG TAGGAAGAGA AACAGTTAGT TCGGGCAGTG AGACAGGAAG ACGGTCCAGG 
AGAAGAGAGA GTGGCAGCGA TGTGATTGAC TCTAGCAGTG AGGCACGTGG ATATTACAGC 
AAGAGGAAAG AGACTGGCAG TGAAATGGGG GGGTCTGGTA GTGAGATAGG GGAAAGATAC 
CAAAATGGCC TAGAACTGGT GTCTGGGTTC AAGAGACTTC CTATCCAGGA TGTGCCAGCC 
AAACCTATAA AAGCTGTTCT GGTGAAACGT GCAGAAAACC AAGAATATGG TTTAAAGTTG 
GGTAGTCAGA TCTTCATTAA GCACATAACA GAAAGTGGAT TGGCAGCTCA AGAGAAGGCA 
TTACAAGAAG GAGACCTCAT TCTAAAGATT AATGGAGTGA CTAGTGAGAA TATGTCATTG 
GCTGACACTC GGAGACTCAT TGAGAAGTCA AGGGGCAAAC TTACCCTCAC TGTTTTGAGA 
GACAATCGAC AGTTCCTGGT TAATATCCCA GAAGTGAGAG ATAGTGAGAG TGAGGGAAGA 
AGCTCCCCTC GAGAGGATAT CTCTGAAATA GGTTCTGAAG ATTTTCCACC ACCATCGGAA 
GCTCCCCCAC CCCCACCAGC ACATCAACAG ATAAGCACGT CTGAGAGTGA TGGTAGTGCC 
TCGGAAGAGC AAGGAATGAA TGGATCAGAA GCTGTGCAAA AAATGGAAAA TTCCAAAGTT 
GAGATTGCTG TAAAAAATGA AGAAGCACAG GTGGTGTCGA CATACAGAGA GGAATCAGTG 
AATCCCAATA ACAACACGAG AGAAGACTTG AGAACAGGGT ACAGTCCGGA TGCTCGTGTA 
ATTCAGTTCA TGAAAGAAAA GAGTATTGGT CTGCGTCTTG CAGGTGGAAA TGACGTGGGA 
ATATTTGTGG CTGCCGTACA GGCAGCAAGC CCTGCAGAAC GAGAAGGAAT GAAAGAGGGT 
GATCAGATCC TGCAGGTGAA TGATACAAGC TTCCATAACT TGACCCGTGA GGATGCTGTG 
CAGTATTTAA TGGGCCTTCC ACAAAACGAG GATGTCATAT TCCTTACACA GGGCAAAGAA 
GACATTTACA GAAAAATGAT TAAATCCAAT GTTGGAGATT CCTTTTATAT CCGTACACAC 
TTCGACTATG AGTCAGATGC TCCATCCGGC CTAAGTTTTA CTCGAGGAGA AATATTTCAT 
ATCGTAGACA CAATGTATCG TGGGAAGATA GGAAGCTGGC TAGCTGTCCG TGTTGCCAGG 
GATTTAAAGG AAAAGGAAAA GGGAATAATA CCAAACAACA ACAGGGCAGA GCAGTATGCC 
AGTTTGGAAC CAGTACTAAA GCCACAAAGC TCCAGTGGAC AGAGGGCAGA GTTCTGGAAG 
TTAAGGGGCC TGCGTGGAGC TAAGAAATTA CTAAGGAAGA GTAGAGAAGA TTTAGCTGCA 
CTGAGCAAGA AGATTAAATA TCCTCCATAT GAAAGAGTGG TGCTTCGGGA AGCAAGCTTT 
AAAAGACCTG TAGTTGTTAT GGGTCCAATC ACAGATATTG CTCATCAGAA GCTTTGTGCA 
GATTTGCCCC AAGAGTTTGA GTCTGCAGAG AGTGTCACGC GAGATGGAGG AGGGACCTCT 
AAGGTCATTA AACTGGATGT TGTAAGGGAA ATTGCTGCAA AGAATAAGCA TGCACTCCTT 
GAGATCACTC CAACAGCTGT AGAACATCTT AACTACATGC AGTTCTATCC TATTGTGGTA 
TTTTGTAACC CTGAAAACAG ACAAGGAGTA AAAGCCATGA GGAAATGGCT GCTTCCTGAC 
TCGAGAAAGA GTTCGAGGCG TTTGTATGCA CAAGCTGTTA AGCAGCGCAA GAACAACTTT 
CATCTATTTA CTGCCACAAT AAATCTGAGT GGAGCAAGTG ACTCATGGCT TCGCAGCTTG 
AAAGAAATTA TTCAAACTGA GCAAACAAAA CCAATATGGA CAACAGAGGA TAAGGAGAAT 
ACAATCACAG AGCCACTGGA TCTCCTGGAT GACTCCACAG CTGAAGTCAA TGACTACCTC 
AGTTGTGATA GCCGTGCAAA CAGTGACTAT GAGGAAACTG ATGCTGAGGG AGGAGCTTAT 
ACTGACCAGG AACTGGAAGA AGAAATTCAA GAGCCAGCAC TTGCTAGGTC TTCTGAACCA 
GTGCTGGATT ACCAGTCAAC CTATTACAGA GCCCATTCGC CAGATGACTA TCCTTCAGAA 
TACAAAGACC AATCTTTGCC TGAGACAAAC AACATCCATG GAACCAAGCC AAGACCTGAA 
TTAAGTTCTA ACTACAGAAG TTCTTTACCA TCTAATGATG ACAGCCCTCA ACAGGAATCC 
GTAAGCCAGC CACAAGGCCA ATGGAGTGCA AGTGCTATGA ACAGTCGAGA ATATGAACAC 
GATGCTCTGC GCAAAAAGTT CAACAATGCA AGATATTCCA GCTCAGATGA GGAGGAAGAT 
GCTTGGGGTG GGCCTGCAAC TGACCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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tjp3 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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