Nubp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nubp1
Entrez Gene ID	26425
Full Name	nucleotide binding protein 1
General protein information	Preferred Names nucleotide binding protein 1 Names cytosolic Fe-S cluster assembly factor NUBP1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A1\|16 5.61 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011955.2	NP_036085.1	cytosolic Fe-S cluster assembly factor NUBP1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NUBP1	NP_002475.2
Danio rerio (zebrafish)	nubp1	NP_001017538.1
Caenorhabditis elegans (roundworm)	F10G8.6	NP_492653.2
Saccharomyces cerevisiae (baker's yeast)	NBP35	NP_011424.3
Schizosaccharomyces pombe (fission yeast)	SPAC637.08	NP_594626.1
Mus musculus (house mouse)	Nubp1	NP_036085.1
Anopheles gambiae (African malaria mosquito)	NUBP1_ANOGA	XP_320535.3
Kluyveromyces lactis	KLLA0E18987g	XP_454809.1
Oryza sativa (rice)	Os02g0601100	NP_001047347.1
Bos taurus (cattle)	NUBP1	NP_001068763.1
Gallus gallus (chicken)	NUBP1	NP_001185570.1
Rattus norvegicus (Norway rat)	Nubp1	NP_001009619.1
Drosophila melanogaster (fruit fly)	CG17904	NP_609805.1
Eremothecium gossypii	AGOS_AFL060W	NP_985488.2
Arabidopsis thaliana (thale cress)	NBP35	NP_568748.1
Xenopus tropicalis (tropical clawed frog)	nubp1	NP_001015835.1
Pan troglodytes (chimpanzee)	NUBP1	XP_001139497.1
Canis lupus familiaris (dog)	NUBP1	XP_536975.1
Magnaporthe oryzae (rice blast fungus)	MGG_06151	XP_003712030.1
Neurospora crassa	NCU08825	XP_962036.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel family of katanin-like 2 protein isoforms (KATNAL2), interacting with nucleotide-binding proteins Nubp1 and Nubp2, are key regulators of different MT-based processes in mammalian cells.
Ververis A, Christodoulou A, Christoforou M, Kamilari C, Lederer CW, Santama N
Cellular and molecular life sciences : CMLS73(1)163-84(2016 Jan)

The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis.
Kypri E, Christodoulou A, Maimaris G, Lethan M, Markaki M, Lysandrou C, Lederer CW, Tavernarakis N, Geimer S, Pedersen LB, Santama N
Cellular and molecular life sciences : CMLS71(3)517-38(2014 Feb)

Nubp1 is required for lung branching morphogenesis and distal progenitor cell survival in mice.
Schnatwinkel C, Niswander L
PloS one7(9)e44871(2012)

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
Nakano Y, Jahan I, Bonde G, Sun X, Hildebrand MS, Engelhardt JF, Smith RJ, Cornell RA, Fritzsch B, B?nfi B
PLoS genetics8(10)e1002966(2012)

Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.
Okuno T, Yamabayashi H, Kogure K
Molecular biology reports37(3)1165-8(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

KATNAL2 isoforms interact with themselves, with each other and with Nubp1 and Nubp2.
Title: A novel family of katanin-like 2 protein isoforms (KATNAL2), interacting with nucleotide-binding proteins Nubp1 and Nubp2, are key regulators of different MT-based processes in mammalian cells.

The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis.
Title: The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis.

Nubp1 represents an essential protein for lung progenitor survival by coordinating vital cellular processes including cell polarity and centrosomal dynamics
Title: Nubp1 is required for lung branching morphogenesis and distal progenitor cell survival in mice.

The C-terminal domain or motif of Nubp1 plays an important role in regulation of localization of Nubp1 protein.
Title: Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.

KIFC5A interacts with nucleotide-binding proteins 1 and 2 (Nubp1 and Nubp2). Nubp1 and Nubp2 also interact with each other.
Title: Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication.

The following Nubp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nubp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14112	NM_011955.2 Latest version!	Mus musculus nucleotide binding protein 1 (Nubp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nubp1 ( NM_011955.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_036085.1 Mus musculus nucleotide binding protein 1 (Nubp1), mRNA.

Price & Availability↑

CloneID	OMu14112
Clone ID Related Accession (Same CDS sequence)	NM_011955.2
Accession Version	NM_011955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	cytosolic Fe-S cluster assembly factor NUBP1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ183745.1, CX210766.1, BU614991.1, BM938167.1 and BI134234.1. On Aug 12, 2009 this sequence version replaced NM_011955.1. ##Evidence-Data-START## Transcript exon combination :: BC031430.1, BC055436.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGG CGCCCCACGG CTGCCCCGGG GCCGACAGCG CCCAGGCCGG CCGAGGAGCC 
TCGTGCCAGG GATGCCCCAA CCAGAGGCTG TGCGCATCCG GAGCCGGCGC TGCTCCGGAT 
CCCGCCGTGG AGGAAATCAG AGAGAAGATG AAGACGGTGA GACACAAGCT CTTGGTGCTG 
TCTGGGAAAG GCGGCGTTGG GAAAAGCACG TTCAGTGCAC ACCTCGCCCA CGGCCTAGCG 
GAGGATGGAG ATACACAGGT TGCTCTTCTG GACATCGATA TCTGTGGGCC ATCGATTCCC 
AAGATCATGG GCTTAGAAGG AGAGCAGGTT CACCAGAGCG GCTCCGGCTG GTCTCCAGTG 
TACGTGGATG ACAATCTGGG GGTGATGTCA GTGGGCTTCC TGCTTAGCAG CCCAGATGAT 
GCTGTCATCT GGAGGGGACC AAAGAAAAAT GGTATGATCA AGCAGTTCCT CCGTGATGTA 
GACTGGGGAG ACGTGGACTA CCTCATCGTA GACACCCCGC CTGGCACGTC TGACGAACAC 
CTCTCAGTGG TCCAGTACCT GGCTGCGGCA CACATTGATG GGGCAGTGAT CCTCACCACC 
CCTCAGGAGG TGGCCCTCCA GGATGTCCGG AAAGAGATCA GCTTCTGCCA CAAGGTGAAG 
CTGCCCATCA TTGGTGTGGT AGAAAACATG AGCGGTTTCA TCTGCCCCAA GTGCAAGAAA 
GAGTCTCAGA TCTTCCCTCC CACGACTGGG GGTGCAGAGG CCATGTGCCA GGATCTGAGG 
ATTCCACTTC TGGGCAAAGT GCCGCTGGAT CCACACATAG GCAAGAGCTG TGACAAAGGC 
CAGTCTTTTT TTGTCGAAGC TCCAGATTCA CCAGCCACAG CAGCCTACAG AAGTATAATT 
CAGAGGATCC GAGACTTTTG TAATTCTCAT CAGTCACATG CTGAGACCCT CATCAGTCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036085.1
CDS	40..1002
Translation	MEEAPHGCPGADSAQAGRGASCQGCPNQRLCASGAGAAPDPAVEEIREKMKTVRHKLLVLSGKGGVGKSTFSAHLAHGLAEDGDTQVALLDIDICGPSIPKIMGLEGEQVHQSGSGWSPVYVDDNLGVMSVGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGDVDYLIVDTPPGTSDEHLSVVQYLAAAHIDGAVILTTPQEVALQDVRKEISFCHKVKLPIIGVVENMSGFICPKCKKESQIFPPTTGGAEAMCQDLRIPLLGKVPLDPHIGKSCDKGQSFFVEAPDSPATAAYRSIIQRIRDFCNSHQSHAETLISP

Target ORF information:

RefSeq Version	NM_011955.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus nucleotide binding protein 1 (Nubp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011955.2

ORF Insert Sequence:

ATGGAGGAGG CGCCCCACGG CTGCCCCGGG GCCGACAGCG CCCAGGCCGG CCGAGGAGCC 
TCGTGCCAGG GATGCCCCAA CCAGAGGCTG TGCGCATCCG GAGCCGGCGC TGCTCCGGAT 
CCCGCCGTGG AGGAAATCAG AGAGAAGATG AAGACGGTGA GACACAAGCT CTTGGTGCTG 
TCTGGGAAAG GCGGCGTTGG GAAAAGCACG TTCAGTGCAC ACCTCGCCCA CGGCCTAGCG 
GAGGATGGAG ATACACAGGT TGCTCTTCTG GACATCGATA TCTGTGGGCC ATCGATTCCC 
AAGATCATGG GCTTAGAAGG AGAGCAGGTT CACCAGAGCG GCTCCGGCTG GTCTCCAGTG 
TACGTGGATG ACAATCTGGG GGTGATGTCA GTGGGCTTCC TGCTTAGCAG CCCAGATGAT 
GCTGTCATCT GGAGGGGACC AAAGAAAAAT GGTATGATCA AGCAGTTCCT CCGTGATGTA 
GACTGGGGAG ACGTGGACTA CCTCATCGTA GACACCCCGC CTGGCACGTC TGACGAACAC 
CTCTCAGTGG TCCAGTACCT GGCTGCGGCA CACATTGATG GGGCAGTGAT CCTCACCACC 
CCTCAGGAGG TGGCCCTCCA GGATGTCCGG AAAGAGATCA GCTTCTGCCA CAAGGTGAAG 
CTGCCCATCA TTGGTGTGGT AGAAAACATG AGCGGTTTCA TCTGCCCCAA GTGCAAGAAA 
GAGTCTCAGA TCTTCCCTCC CACGACTGGG GGTGCAGAGG CCATGTGCCA GGATCTGAGG 
ATTCCACTTC TGGGCAAAGT GCCGCTGGAT CCACACATAG GCAAGAGCTG TGACAAAGGC 
CAGTCTTTTT TTGTCGAAGC TCCAGATTCA CCAGCCACAG CAGCCTACAG AAGTATAATT 
CAGAGGATCC GAGACTTTTG TAATTCTCAT CAGTCACATG CTGAGACCCT CATCAGTCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel family of katanin-like 2 protein isoforms (KATNAL2), interacting with nucleotide-binding proteins Nubp1 and Nubp2, are key regulators of different MT-based processes in mammalian cells.
Cellular and molecular life sciences : CMLS73(1)163-84(2016 Jan)
Ververis A,Christodoulou A,Christoforou M,Kamilari C,Lederer CW,Santama N

The nucleotide-binding proteins Nubp1 and Nubp2 are negative regulators of ciliogenesis.
Cellular and molecular life sciences : CMLS71(3)517-38(2014 Feb)
Kypri E,Christodoulou A,Maimaris G,Lethan M,Markaki M,Lysandrou C,Lederer CW,Tavernarakis N,Geimer S,Pedersen LB,Santama N

Nubp1 is required for lung branching morphogenesis and distal progenitor cell survival in mice.
PloS one7(9)e44871(2012)
Schnatwinkel C,Niswander L

A mutation in the Srrm4 gene causes alternative splicing defects and deafness in the Bronx waltzer mouse.
PLoS genetics8(10)e1002966(2012)
Nakano Y,Jahan I,Bonde G,Sun X,Hildebrand MS,Engelhardt JF,Smith RJ,Cornell RA,Fritzsch B,B?nfi B

Comparison of intracellular localization of Nubp1 and Nubp2 using GFP fusion proteins.
Molecular biology reports37(3)1165-8(2010 Mar)
Okuno T,Yamabayashi H,Kogure K

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Nubp1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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