Olfr544 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Olfr544
Entrez Gene ID	257926
Full Name	olfactory receptor 544
Synonyms	MOR42-3,Ors6
General protein information	Preferred Names olfactory receptor 544 Names olfactory receptor 544 G-protein-coupled receptor GA_x6K02T2PBJ9-5206624-5205620 odorant receptor S6 odorant receptor S79 olfactory receptor MOR42-3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E3
Summary	Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_020289.2	NP_064685.2	olfactory receptor 544

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	or113-2	NP_001124289.1
Xenopus tropicalis (tropical clawed frog)	LOC100486026	XP_002945173.1
Xenopus tropicalis (tropical clawed frog)	LOC100487752	XP_002942415.2
Xenopus tropicalis (tropical clawed frog)	LOC100488858	XP_002942422.1
Xenopus tropicalis (tropical clawed frog)	LOC100487277	XP_002942412.1
Xenopus tropicalis (tropical clawed frog)	LOC100488220	XP_002942418.1
Xenopus tropicalis (tropical clawed frog)	LOC100488070	XP_002942417.1
Danio rerio (zebrafish)	LOC100535375	XP_003200051.1
Xenopus tropicalis (tropical clawed frog)	LOC100486975	XP_002942410.1
Xenopus tropicalis (tropical clawed frog)	LOC100488379	XP_002942419.1
Mus musculus (house mouse)	Olfr544	NP_064685.2
Xenopus tropicalis (tropical clawed frog)	LOC100488533	XP_002942420.1
Rattus norvegicus (Norway rat)	Olr39	NP_001000128.1
Danio rerio (zebrafish)	or114-1	NP_001122054.1
Danio rerio (zebrafish)	or113-1	NP_001124274.1
Xenopus tropicalis (tropical clawed frog)	LOC100489177	XP_002942424.1
Xenopus tropicalis (tropical clawed frog)	LOC100489019	XP_002942423.1
Xenopus tropicalis (tropical clawed frog)	LOC100487582	XP_002942414.1
Xenopus tropicalis (tropical clawed frog)	LOC100487911	XP_002942416.2
Xenopus tropicalis (tropical clawed frog)	LOC100487429	XP_002942413.1

Gene Ontology
Bibliography

Related articles in PubMed

Olfactory receptor 544 reduces adiposity by steering fuel preference toward fats.
Wu C, Hwang SH, Jia Y, Choi J, Kim YJ, Choi D, Pathiraja D, Choi IG, Koo SH, Lee SJ
The Journal of clinical investigation127(11)4118-4123(2017 Nov)

Molecular determinants of the olfactory receptor Olfr544 activation by azelaic acid.
Thach TT, Hong YJ, Lee S, Lee SJ
Biochemical and biophysical research communications485(2)241-248(2017 04)

Functional Role of the C-Terminal Amphipathic Helix 8 of Olfactory Receptors and Other G Protein-Coupled Receptors.
Sato T, Kawasaki T, Mine S, Matsumura H
International journal of molecular sciences17(11)(2016 Nov)

The N-terminal acidic residue of the cytosolic helix 8 of an odorant receptor is responsible for different response dynamics via G-protein.
Kawasaki T, Saka T, Mine S, Mizohata E, Inoue T, Matsumura H, Sato T
FEBS letters589(10)1136-42(2015 Apr)

Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues.
Kang N, Kim H, Jae Y, Lee N, Ku CR, Margolis F, Lee EJ, Bahk YY, Kim MS, Koo J
PloS one10(1)e0116097(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Olfr544 orchestrates the metabolic interplay between the liver and adipose tissue, mobilizing stored fats from adipose tissue and shifting the fuel preference to fats in the liver and brown adipose tissue.
Title: Olfactory receptor 544 reduces adiposity by steering fuel preference toward fats.

These data indicated that a hydrogen bond between Y109 residue and azelaic acid is a major determinant of the Olfr544-azelaic acid interaction and activation.
Title: Molecular determinants of the olfactory receptor Olfr544 activation by azelaic acid.

Data indicate that the hydrophobic core buried between helix 8 and TM1-2 of olfactory receptor (mOR-S6) is important for the activation of both G protein alpha 15 (Galpha15).
Title: Functional Role of the C-Terminal Amphipathic Helix 8 of Olfactory Receptors and Other G Protein-Coupled Receptors.

The N-terminal hydrophobic core between helix 8 and TM1-2 of mOR-S6 is important for activation of both Galpha15_olf and Galpha15.
Title: The N-terminal acidic residue of the cytosolic helix 8 of an odorant receptor is responsible for different response dynamics via G-protein.

Three olfactory receptors (olfr544, olfr558, and olfr1386) were expressed in the olfactory marker protein (+) cells of the bladder and thyroid.
Title: Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues.

The following Olfr544 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Olfr544 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu25252	NM_020289.2 Latest version!	Mus musculus olfactory receptor 544 (Olfr544), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Olfr544 ( NM_020289.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064685.2 Mus musculus olfactory receptor 544 (Olfr544), mRNA.

Price & Availability↑

CloneID	OMu25252
Clone ID Related Accession (Same CDS sequence)	NM_020289.2
Accession Version	NM_020289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-23
Organism	Mus musculus(house mouse)
Product	olfactory receptor 544
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC132096.4. On Nov 18, 2009 this sequence version replaced NM_020289.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DR065630.1, DR065624.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCAGGGT GGAGCAATGG CACCTACAAT GAGTCCTACA CCAGCTTCCT CCTCATGGGC 
TTCCCAGGGA TGCAGGAAGC CAGAGCCCTC CTGGTGCTGC CCTTCCTCAG CCTCTACCTG 
GTGATCCTCT TCACCAATGC CCTGGTCATC CACACGGTGG CATCCCAGCG CAGCCTGCAC 
CAGCCCATGT ACCTGCTCAT TGCCCTGCTC CTGGCTGTCA ATATCTGCGC TGCCACCACC 
GTGGTGCCCC CCATGCTCTT CAGCTTCTCC ACACGCTTCA ACCGCATCTC CCTCCCTCGA 
TGCTTGGGAC AGATGTTCTG CATCTACTTC CTTATTGTCT TTGACTGCAA CATCCTCCTG 
GTCATGGCTC TAGATCGCTA TGTGGCTATC TGCTACCCTC TCCGCTACCC AGAAATAGTG 
ACAGGACAGT TACTGGCTGG TCTGGTGGTG CTGGCAGTCA CCAGGAGCAC ATGCATTGTT 
GCTCCAGTGG TGGTGCTGGC CTCGCGGGTT CGCTTCTGTC GCTCAGATGT GATCCGCCAC 
TTTGCCTGTG AGCACATGGC CCTGATGAAG CTTTCCTGTG GGGACATCTC GCTGAATAAG 
ACGGTGGGAC TCACTGTTCG CATCTTCAAC CGAGTCCTGG ATATGCTCCT GTTAGGTGCC 
TCCTACTCCC GCATCATCCA TGCTGCCTTC AGGATCTCAT CAGGTGGAGC ACGGTCCAAA 
GCCCTGAACA CCTGTGGCTC CCACCTGCTG GTCATCTTCA CTGTCTACTC CTCCACCATG 
TCCTCATCCA TTGTCTACCG TGTGGCGCGC ACTGCCTCCC AAGATGTGCA CAACTTGCTC 
AGTGCTTTCT ATCTGTTGCT CCCGTGTCTG GTCAACCCCA TCATCTACGG GGCCAGAACC 
AAGGAAATCA GGCAGCACCT GGTAGCTCTG TTCCAAAGGA CTCAGCAACA GGTCTTCACT 
GAGAAGCCCC AGTCCCTGCC CTCGAATAGA GAGCTTCCTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064685.2
CDS	177..1181
Translation	MSGWSNGTYNESYTSFLLMGFPGMQEARALLVLPFLSLYLVILFTNALVIHTVASQRSLHQPMYLLIALLLAVNICAATTVVPPMLFSFSTRFNRISLPRCLGQMFCIYFLIVFDCNILLVMALDRYVAICYPLRYPEIVTGQLLAGLVVLAVTRSTCIVAPVVVLASRVRFCRSDVIRHFACEHMALMKLSCGDISLNKTVGLTVRIFNRVLDMLLLGASYSRIIHAAFRISSGGARSKALNTCGSHLLVIFTVYSSTMSSSIVYRVARTASQDVHNLLSAFYLLLPCLVNPIIYGARTKEIRQHLVALFQRTQQQVFTEKPQSLPSNRELPG

Target ORF information:

RefSeq Version	NM_020289.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus olfactory receptor 544 (Olfr544), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020289.2

ORF Insert Sequence:

ATGTCAGGGT GGAGCAATGG CACCTACAAT GAGTCCTACA CCAGCTTCCT CCTCATGGGC 
TTCCCAGGGA TGCAGGAAGC CAGAGCCCTC CTGGTGCTGC CCTTCCTCAG CCTCTACCTG 
GTGATCCTCT TCACCAATGC CCTGGTCATC CACACGGTGG CATCCCAGCG CAGCCTGCAC 
CAGCCCATGT ACCTGCTCAT TGCCCTGCTC CTGGCTGTCA ATATCTGCGC TGCCACCACC 
GTGGTGCCCC CCATGCTCTT CAGCTTCTCC ACACGCTTCA ACCGCATCTC CCTCCCTCGA 
TGCTTGGGAC AGATGTTCTG CATCTACTTC CTTATTGTCT TTGACTGCAA CATCCTCCTG 
GTCATGGCTC TAGATCGCTA TGTGGCTATC TGCTACCCTC TCCGCTACCC AGAAATAGTG 
ACAGGACAGT TACTGGCTGG TCTGGTGGTG CTGGCAGTCA CCAGGAGCAC ATGCATTGTT 
GCTCCAGTGG TGGTGCTGGC CTCGCGGGTT CGCTTCTGTC GCTCAGATGT GATCCGCCAC 
TTTGCCTGTG AGCACATGGC CCTGATGAAG CTTTCCTGTG GGGACATCTC GCTGAATAAG 
ACGGTGGGAC TCACTGTTCG CATCTTCAAC CGAGTCCTGG ATATGCTCCT GTTAGGTGCC 
TCCTACTCCC GCATCATCCA TGCTGCCTTC AGGATCTCAT CAGGTGGAGC ACGGTCCAAA 
GCCCTGAACA CCTGTGGCTC CCACCTGCTG GTCATCTTCA CTGTCTACTC CTCCACCATG 
TCCTCATCCA TTGTCTACCG TGTGGCGCGC ACTGCCTCCC AAGATGTGCA CAACTTGCTC 
AGTGCTTTCT ATCTGTTGCT CCCGTGTCTG GTCAACCCCA TCATCTACGG GGCCAGAACC 
AAGGAAATCA GGCAGCACCT GGTAGCTCTG TTCCAAAGGA CTCAGCAACA GGTCTTCACT 
GAGAAGCCCC AGTCCCTGCC CTCGAATAGA GAGCTTCCTG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Olfactory receptor 544 reduces adiposity by steering fuel preference toward fats.
The Journal of clinical investigation127(11)4118-4123(2017 Nov)
Wu C,Hwang SH,Jia Y,Choi J,Kim YJ,Choi D,Pathiraja D,Choi IG,Koo SH,Lee SJ

Molecular determinants of the olfactory receptor Olfr544 activation by azelaic acid.
Biochemical and biophysical research communications485(2)241-248(2017 04)
Thach TT,Hong YJ,Lee S,Lee SJ

Functional Role of the C-Terminal Amphipathic Helix 8 of Olfactory Receptors and Other G Protein-Coupled Receptors.
International journal of molecular sciences17(11)(2016 Nov)
Sato T,Kawasaki T,Mine S,Matsumura H

The N-terminal acidic residue of the cytosolic helix 8 of an odorant receptor is responsible for different response dynamics via G-protein.
FEBS letters589(10)1136-42(2015 Apr)
Kawasaki T,Saka T,Mine S,Mizohata E,Inoue T,Matsumura H,Sato T

Olfactory marker protein expression is an indicator of olfactory receptor-associated events in non-olfactory tissues.
PloS one10(1)e0116097(2015)
Kang N,Kim H,Jae Y,Lee N,Ku CR,Margolis F,Lee EJ,Bahk YY,Kim MS,Koo J

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Olfr544 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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