Bicra cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bicra
Entrez Gene ID	243842
Full Name	BRD4 interacting chromatin remodeling complex associated protein
Synonyms	Gltscr1
General protein information	Preferred Names BRD4 interacting chromatin remodeling complex associated protein Names glioma tumor suppressor candidate region gene 1 protein glioma tumor suppressor candidate region gene 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A2\|7 8.67 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011250559.2	XP_011248861.1	glioma tumor suppressor candidate region gene 1 protein isoform X3
NM_001081418.1	NP_001074887.1	BRD4-interacting chromatin-remodeling complex-associated protein
XM_006539917.3	XP_006539980.1	glioma tumor suppressor candidate region gene 1 protein isoform X1
XM_006539918.3	XP_006539981.1	glioma tumor suppressor candidate region gene 1 protein isoform X2
XM_030242555.1	XP_030098415.1	BRD4-interacting chromatin-remodeling complex-associated protein isoform X4
XM_006539918.4	XP_006539981.1	BRD4-interacting chromatin-remodeling complex-associated protein isoform X2
XM_011250559.3	XP_011248861.1	BRD4-interacting chromatin-remodeling complex-associated protein isoform X3
XM_006539917.4	XP_006539980.1	BRD4-interacting chromatin-remodeling complex-associated protein isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	gltscr1	NP_001107299.1
	GLTSCR1	XP_002801361.1
Gallus gallus (chicken)	LOC101749950	XP_004950244.1
Homo sapiens (human)	GLTSCR1	NP_056526.3
Mus musculus (house mouse)	Gltscr1	NP_001074887.1
Pan troglodytes (chimpanzee)	GLTSCR1	XP_003316527.1
Canis lupus familiaris (dog)	GLTSCR1	XP_005616924.1
Rattus norvegicus (Norway rat)	Gltscr1	NP_001099696.2

Gene Ontology
Bibliography

Related articles in PubMed

The gene for soluble N-ethylmaleimide sensitive factor attachment protein alpha is mutated in hydrocephaly with hop gait (hyh) mice.
Hong HK, Chakravarti A, Takahashi JS
Proceedings of the National Academy of Sciences of the United States of America101(6)1748-53(2004 Feb)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Mapping of the mouse hyh gene to a YAC/BAC contig on proximal Chromosome 7.
Chae TH, Allen KM, Davisson MT, Sweet HO, Walsh CA
Mammalian genome : official journal of the International Mammalian Genome Society13(5)239-44(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bicra gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bicra cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu89489	XM_030242555.1 Latest version!	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu02393	XM_006539917.4 Latest version!	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu38222	XM_006539918.4 Latest version!	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu74158	XM_011250559.3 Latest version!	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu02393	NM_001081418.1 Latest version!	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OMu38222	XM_006539918.3	Mus musculus glioma tumor suppressor candidate region gene 1 (Gltscr1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu74158	XM_011250559.2	Mus musculus glioma tumor suppressor candidate region gene 1 (Gltscr1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OMu02393	XM_006539917.3	Mus musculus glioma tumor suppressor candidate region gene 1 (Gltscr1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bicra ( XM_030242555.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098415.1 Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu89489
Clone ID Related Accession (Same CDS sequence)	XM_030242555.1
Accession Version	XM_030242555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	BRD4-interacting chromatin-remodeling complex-associated protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTCCAT GTGCAGGAAG CTGCCGCCAA CCACCTAAAC 
CCTGAGCCCA GCCAGCCTGC CCCCAGCGTG GACCTGGACT TCCTAGAAGA TGATATCTTG 
GGCTCCCCTG CAGCAGGAGG AGGTGGAGGG GGCGGCGGGG CCCCAGACCA GCCCTGTGAC 
ATCCTTCAGC AGAGTCTTCA GGAGGCCAAC ATCACAGAAC AGACCCTGGA GGCTGAGGCT 
GAACTGGACC TGGGCCCCTT CCAGCTGCCC ACCCTACAGC CCGCTGACAA TGGGGCAGGT 
GCTACTGGAG CCGCAGGAGC CACGGCAGTG ACTGCAGGAC CCCAGGCTCT CTTCCCAGGC 
AGCGCGGATC TGCTGGGGCT GCAAGCCCCG CCCACTGTAC TGACCCACCA GGCCCTGGTG 
CCACCCCAGG ATGTGGTCAA CAAGGCCTTG AGCGTCCAGC CCTTCCTGCA GCCTGTGGGC 
CTGGGCAATG TGACCCTTCA GCCCATTTCA GGCCTCCAGG GCCTTCCCAA TGGCAGTCCT 
GGGAATGCTG CAGCAGCCAC CTTGGGTCTG ACACCTATTC AAGTGGTGGG CCAGCCCGTC 
ATGGCTCTCA ACCCACCCAC CTCCCAGCTC TTGGCAAAGC AGGTACCTGT CAGTGGCTAC 
CTGGCCTCAG CAGCTGGTCC TTCAGAGCCA GTGACACTGG CATCTGCCGG CGTGTCCCCC 
CAGGGAGCCG GCCTGGTCAT CCAGAAAAAT CTTCCAGCCG CAGTGACCAC CACACTCAAC 
GGGAACTCGG TGTTTGCCGG GACAGGGGCT GCCACTGCAG CAGCCAGTGG GGCACCCTCG 
GGACAGCCGC TGGCGGTGGC CCCGGGCCTT GGCACATCAC CACTGGTACA AGCACCCAGT 
GTGATTTTAC ACAGAACCCC TACGCCTATC CAGCCCAAGC CTACAGGGGT CCTGCCCTCC 
AAACTCTACC AGCTGACACC CAAGCCCTTT CCCCCTACCG GAGCCACCCT TACCATCCAG 
GGTGAACCAG GCACCTTGCC CCAGCAGCCT AAGGCCCCCC AGAACCTGAC TTTTATGGCC 
ACGGGCAAAG CTGGCCAGAA TGTGGTGCTG TCTGGCTTCC CGGCACCGGC TTTGCAGGCG 
AATGTGTTCA AGCAGCCACC AGTCACCACC ACGGGGACAG CCCCGCCACA GCCACCAGGG 
GCCCTCAGCA AACCCATGAG CGTCCACCTC CTCAATCAAG GCAGCAGCAT CGTGATCCCA 
GCCCAGCACA TGCTGCCTGG CCAGAACCAG TTCTTGCTGC CAGGCACCCC AGCCGTACAA 
CTCCCTCAGT CACTCTCTGC ACTGCCTGCC AACGTGGGAG GCCAGATCCT CACAGCTGCA 
GCACCACACG CAGGTGGACA GCTCATTGCC AACCCTATCC TCACCAACCA GAACCTGGCA 
GGCCCACTGA GTCTGGGCCC AGTGCTGGCA CCCCACTCTG GGGCACACAG CGCTGCACAC 
ATCCTCTCTG CAGCTCCCAT CCAGGTGGGC CAGCCTGCCC TCTTCCAGAT GCCTGTGTCA 
CTGGCCACTG GCAGCCTGCC TACTCAGAGC CAGCCGGCTC CCACTGGCCC CACAGCCACC 
ACCGTCCTCC AGGGCGTCAC CCTGCCTCCC AGTGCTGTGG CCATGCTTAA CACGCCTGAT 
GGGCTAGTGC AACCCTCCAC TCCAGCTGCC ACCACTGGGG AGGCCACACC AGTTCTGGCC 
GTTCAGCCTG CAACCCAGGT GCCCCCTGCT GTCACCACAC CACTGCCTAT GGGTCTCCAA 
CAGCCACAGG CACAGCAGCC TCCACAGGTC CCTACTCCAC AGGCGGCCAC CCAGCCTCAG 
GCCACCCCTC CTCAGGCCAG CCCAAGCCTG GCTTCCAGCC CAGAGAAGAT AGTCCTGGGG 
CAGGCGCCCC CTGCGGCCAC AACGGCCATC CTCACTCAGG ATTCCCTACA GATGTTCCTG 
CCCCAGATCC CTGCAGCTGC TCCACTGAAA GCCCCTGGCC CCGCCTCCTC CCCCTCACTA 
CCTCACCAGG CCCCCCTGGG AGACAGTCCC CACATGCCCT CCCCACACCC TGCCAGGCCC 
CCTTCCCGCC CACCCTCAAG ACCCCACTCA CGCCCTCCAT CCCAGCCCCA GAGCCTGACC 
TGCCCACCCT CTGAGCCCAC CCTGCACCCT TGCCCTCCAC CCCAGGGTCC CCCAACTCTA 
CCTGGCATCT TTGTCATCCA GAATCAATTG GGCGCCCCAC CACCAGCCAG CACCCCAGCC 
TCCACAGCCC CGGGCCCACC CCAGCCTCCT CTGCGACCCC CATCCCAGCC TCCAGAGGGC 
CCACTGCCCC CAGCCTCCCA CCTCCCTCCT GCCTCCACCC CCTCGGCCGT GGCCTCCTCC 
TCTGAGCCTT CTGCCAGGTT GCCGGTCCCC ACACCCCCTG ACTTCCAACT CCAGTTCCCA 
CCGAGCCAGG GACCCCATAA GTCCCCTACT CCGCCACCAG CCCTCCACAT GGTCCCTGAG 
CCCACGGCAC CCCCTCCTCC ACCACCTCGG ACCTTCCAGA TGGTAACCGC CCCCTTCCCA 
GCGTTGCCCC AGCCAAAAGC ACTTCTGGAA CGATTCCACC AGGTGCCATC TGGGATTATT 
CTCCAGAATA AGGCTGGGGG TACTCCCACC ACCCCACAGA CATCCACCAC CCTGGGGACC 
CTCACCGGTC CTACTGCCTC TGTGCTAGTC AGTGGACAGG CACCACCTGG GACTCCTGCC 
GCCTCTAGCC ATGTCCCAGC CTCCACACCT ATGGCCACCA CAGGCCTCCC TCCTCTACTT 
CCTGCCGAAA ACAAAGCTTT TGCCAGCAAC CTTCCAACCC TGAGTGTGGC CAAAGCTACC 
GTGTCTGGGC CAGGGAAGCC CCCAGCAATT CAGTATGACA GCAAGTTGTG TAGCTTGAAG 
AAACAGCCCC TACTGCAACC CAGCAAAGAA GCCTGCTTCC TGGAGCATCT GCACAAACAC 
CAGGGCTCTG TCCTGCACCC CGATTACAAG ACAGCCTTCC CCTCCTTTGA GGACGCCCTC 
CATCGCCTCC TGCCCTACCA TGTCTACCAA GGCGCCCTCC CCTCCCCCAA CGACTACCAT 
AAAGTGGATG AAGAATTTGA GACTGTCTCT ACGCAGCTGC TCAAACGCAC CCAGGCCATG 
CTCAATAAAT ATCGGCTTTT GCTTCTGGAA GAGTCCAGGA GAGTCAGTCC TTCTGCGGAG 
ATGGTTATGA TCGACCGAAT GTTCATTCAG GAGGAGAAGA CCACCCTTGC CTTGGATAAG 
CAGCTTGCCA AGGAGAAGCC TGATGAGTAC GTGTCTTCCT CCCGCTCCCT TGGCTTCCCT 
GTCCCAGTGT CTTCCGAGGG CCACCGGCTC CCCAGCCATG GCCAGTCGTC TTCATCCTCC 
ACATCTGGAA CGTCTGCCCA GCCCCCTCCT CATCTGCCCA CCAAGCTAGT GATCCGGCAC 
GGTGGGGCCG GCGGCTCTCC CTCAGTGACC TGGGCCCGGG CATCCTCCTC CTTGTCATCC 
ACTTCCTCAT CCTCCTCCTC ATCCTCTGCT GCCTCATCCC TGGACGCAGA TGAGGACGGC 
CCCATGCCCA CCCGTAACCG GCCACCCATC AAGACCTATG AGGCCCGGAG CCGCATTGGT 
CTCAAACTCA AGATCAAACA AGAGGCGGGG CTCAGCAAGG TGGTGCACAA CACTGCACTG 
GATCCTGTGC ATCAGCCCTT GCCGGCTCCA ACCCCAGCGA AAGGGGCGGA GCCTCCGCCA 
CACCCAGCTC CGCCCCCACT CCCTCCTGCT ACCCAGGCGC AGATGAATGG CACTCTGGAC 
CATCCCCCAC CCGCAGTACG CAAACCCACG GTGCCTGCGT CCTGCCCACG TCTACCACTA 
CGCAAGACCT ACCGAGAAAA CATGGGCAAT CCTGGTGCCG CCGAGGGTGC ACAGGGACGG 
CCGCGGGGTG CGGGCAGCCC CACCCCACTG CCCACCAAGG TAGACGAAGC CACCAGTGGG 
CTGATCCGGG AGCTGGCAGC GGTGGAGGAT GAACTATATC AGCGGGTTCT GAAGGGCGGC 
CCACCACCCC CGGAGACTCC AGCCTCCGCT ACCAGCCAGG GCCCCACTGA ACCCAGTTGG 
GAAGCACCCG TGCTACCCCC AGCCAAACGA CGCAAGTCTG AGTCCCCGGA CGTGGACCAG 
GCCAGCTTCT CTAGTGACAG CCCGCAGGAT GATACACTTA CTGAGCATTT GCAGAGTGCC 
ATCGACAGCA TCCTTAACCT GCAGCAGGCC CCCGGCCGGA CACCCGCAGG CCCATACCCC 
CATACGGGGC CCACGCCTGG CACCCCCACA TCCCCAGCGC CCCTGCACAG GCCTGACGCC 
TTCCCACCCT CTAGTCACAA TGGTGGCCTC GGTGCCAGGA CGTTGAACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098415.1
CDS	358..4851
Translation	MDDEDGRCLLDVICDPQALNDFLHGSEKLHVQEAAANHLNPEPSQPAPSVDLDFLEDDILGSPAAGGGGGGGGAPDQPCDILQQSLQEANITEQTLEAEAELDLGPFQLPTLQPADNGAGATGAAGATAVTAGPQALFPGSADLLGLQAPPTVLTHQALVPPQDVVNKALSVQPFLQPVGLGNVTLQPISGLQGLPNGSPGNAAAATLGLTPIQVVGQPVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLPAAVTTTLNGNSVFAGTGAATAAASGAPSGQPLAVAPGLGTSPLVQAPSVILHRTPTPIQPKPTGVLPSKLYQLTPKPFPPTGATLTIQGEPGTLPQQPKAPQNLTFMATGKAGQNVVLSGFPAPALQANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTPAVQLPQSLSALPANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAAHILSAAPIQVGQPALFQMPVSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNTPDGLVQPSTPAATTGEATPVLAVQPATQVPPAVTTPLPMGLQQPQAQQPPQVPTPQAATQPQATPPQASPSLASSPEKIVLGQAPPAATTAILTQDSLQMFLPQIPAAAPLKAPGPASSPSLPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPTLPGIFVIQNQLGAPPPASTPASTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVASSSEPSARLPVPTPPDFQLQFPPSQGPHKSPTPPPALHMVPEPTAPPPPPPRTFQMVTAPFPALPQPKALLERFHQVPSGIILQNKAGGTPTTPQTSTTLGTLTGPTASVLVSGQAPPGTPAASSHVPASTPMATTGLPPLLPAENKAFASNLPTLSVAKATVSGPGKPPAIQYDSKLCSLKKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPNDYHKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALDKQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGQSSSSSTSGTSAQPPPHLPTKLVIRHGGAGGSPSVTWARASSSLSSTSSSSSSSSAASSLDADEDGPMPTRNRPPIKTYEARSRIGLKLKIKQEAGLSKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTLDHPPPAVRKPTVPASCPRLPLRKTYRENMGNPGAAEGAQGRPRGAGSPTPLPTKVDEATSGLIRELAAVEDELYQRVLKGGPPPPETPASATSQGPTEPSWEAPVLPPAKRRKSESPDVDQASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPHTGPTPGTPTSPAPLHRPDAFPPSSHNGGLGARTLNR

Target ORF information:

RefSeq Version	XM_030242555.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242555.1

ORF Insert Sequence:

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTCCAT GTGCAGGAAG CTGCCGCCAA CCACCTAAAC 
CCTGAGCCCA GCCAGCCTGC CCCCAGCGTG GACCTGGACT TCCTAGAAGA TGATATCTTG 
GGCTCCCCTG CAGCAGGAGG AGGTGGAGGG GGCGGCGGGG CCCCAGACCA GCCCTGTGAC 
ATCCTTCAGC AGAGTCTTCA GGAGGCCAAC ATCACAGAAC AGACCCTGGA GGCTGAGGCT 
GAACTGGACC TGGGCCCCTT CCAGCTGCCC ACCCTACAGC CCGCTGACAA TGGGGCAGGT 
GCTACTGGAG CCGCAGGAGC CACGGCAGTG ACTGCAGGAC CCCAGGCTCT CTTCCCAGGC 
AGCGCGGATC TGCTGGGGCT GCAAGCCCCG CCCACTGTAC TGACCCACCA GGCCCTGGTG 
CCACCCCAGG ATGTGGTCAA CAAGGCCTTG AGCGTCCAGC CCTTCCTGCA GCCTGTGGGC 
CTGGGCAATG TGACCCTTCA GCCCATTTCA GGCCTCCAGG GCCTTCCCAA TGGCAGTCCT 
GGGAATGCTG CAGCAGCCAC CTTGGGTCTG ACACCTATTC AAGTGGTGGG CCAGCCCGTC 
ATGGCTCTCA ACCCACCCAC CTCCCAGCTC TTGGCAAAGC AGGTACCTGT CAGTGGCTAC 
CTGGCCTCAG CAGCTGGTCC TTCAGAGCCA GTGACACTGG CATCTGCCGG CGTGTCCCCC 
CAGGGAGCCG GCCTGGTCAT CCAGAAAAAT CTTCCAGCCG CAGTGACCAC CACACTCAAC 
GGGAACTCGG TGTTTGCCGG GACAGGGGCT GCCACTGCAG CAGCCAGTGG GGCACCCTCG 
GGACAGCCGC TGGCGGTGGC CCCGGGCCTT GGCACATCAC CACTGGTACA AGCACCCAGT 
GTGATTTTAC ACAGAACCCC TACGCCTATC CAGCCCAAGC CTACAGGGGT CCTGCCCTCC 
AAACTCTACC AGCTGACACC CAAGCCCTTT CCCCCTACCG GAGCCACCCT TACCATCCAG 
GGTGAACCAG GCACCTTGCC CCAGCAGCCT AAGGCCCCCC AGAACCTGAC TTTTATGGCC 
ACGGGCAAAG CTGGCCAGAA TGTGGTGCTG TCTGGCTTCC CGGCACCGGC TTTGCAGGCG 
AATGTGTTCA AGCAGCCACC AGTCACCACC ACGGGGACAG CCCCGCCACA GCCACCAGGG 
GCCCTCAGCA AACCCATGAG CGTCCACCTC CTCAATCAAG GCAGCAGCAT CGTGATCCCA 
GCCCAGCACA TGCTGCCTGG CCAGAACCAG TTCTTGCTGC CAGGCACCCC AGCCGTACAA 
CTCCCTCAGT CACTCTCTGC ACTGCCTGCC AACGTGGGAG GCCAGATCCT CACAGCTGCA 
GCACCACACG CAGGTGGACA GCTCATTGCC AACCCTATCC TCACCAACCA GAACCTGGCA 
GGCCCACTGA GTCTGGGCCC AGTGCTGGCA CCCCACTCTG GGGCACACAG CGCTGCACAC 
ATCCTCTCTG CAGCTCCCAT CCAGGTGGGC CAGCCTGCCC TCTTCCAGAT GCCTGTGTCA 
CTGGCCACTG GCAGCCTGCC TACTCAGAGC CAGCCGGCTC CCACTGGCCC CACAGCCACC 
ACCGTCCTCC AGGGCGTCAC CCTGCCTCCC AGTGCTGTGG CCATGCTTAA CACGCCTGAT 
GGGCTAGTGC AACCCTCCAC TCCAGCTGCC ACCACTGGGG AGGCCACACC AGTTCTGGCC 
GTTCAGCCTG CAACCCAGGT GCCCCCTGCT GTCACCACAC CACTGCCTAT GGGTCTCCAA 
CAGCCACAGG CACAGCAGCC TCCACAGGTC CCTACTCCAC AGGCGGCCAC CCAGCCTCAG 
GCCACCCCTC CTCAGGCCAG CCCAAGCCTG GCTTCCAGCC CAGAGAAGAT AGTCCTGGGG 
CAGGCGCCCC CTGCGGCCAC AACGGCCATC CTCACTCAGG ATTCCCTACA GATGTTCCTG 
CCCCAGATCC CTGCAGCTGC TCCACTGAAA GCCCCTGGCC CCGCCTCCTC CCCCTCACTA 
CCTCACCAGG CCCCCCTGGG AGACAGTCCC CACATGCCCT CCCCACACCC TGCCAGGCCC 
CCTTCCCGCC CACCCTCAAG ACCCCACTCA CGCCCTCCAT CCCAGCCCCA GAGCCTGACC 
TGCCCACCCT CTGAGCCCAC CCTGCACCCT TGCCCTCCAC CCCAGGGTCC CCCAACTCTA 
CCTGGCATCT TTGTCATCCA GAATCAATTG GGCGCCCCAC CACCAGCCAG CACCCCAGCC 
TCCACAGCCC CGGGCCCACC CCAGCCTCCT CTGCGACCCC CATCCCAGCC TCCAGAGGGC 
CCACTGCCCC CAGCCTCCCA CCTCCCTCCT GCCTCCACCC CCTCGGCCGT GGCCTCCTCC 
TCTGAGCCTT CTGCCAGGTT GCCGGTCCCC ACACCCCCTG ACTTCCAACT CCAGTTCCCA 
CCGAGCCAGG GACCCCATAA GTCCCCTACT CCGCCACCAG CCCTCCACAT GGTCCCTGAG 
CCCACGGCAC CCCCTCCTCC ACCACCTCGG ACCTTCCAGA TGGTAACCGC CCCCTTCCCA 
GCGTTGCCCC AGCCAAAAGC ACTTCTGGAA CGATTCCACC AGGTGCCATC TGGGATTATT 
CTCCAGAATA AGGCTGGGGG TACTCCCACC ACCCCACAGA CATCCACCAC CCTGGGGACC 
CTCACCGGTC CTACTGCCTC TGTGCTAGTC AGTGGACAGG CACCACCTGG GACTCCTGCC 
GCCTCTAGCC ATGTCCCAGC CTCCACACCT ATGGCCACCA CAGGCCTCCC TCCTCTACTT 
CCTGCCGAAA ACAAAGCTTT TGCCAGCAAC CTTCCAACCC TGAGTGTGGC CAAAGCTACC 
GTGTCTGGGC CAGGGAAGCC CCCAGCAATT CAGTATGACA GCAAGTTGTG TAGCTTGAAG 
AAACAGCCCC TACTGCAACC CAGCAAAGAA GCCTGCTTCC TGGAGCATCT GCACAAACAC 
CAGGGCTCTG TCCTGCACCC CGATTACAAG ACAGCCTTCC CCTCCTTTGA GGACGCCCTC 
CATCGCCTCC TGCCCTACCA TGTCTACCAA GGCGCCCTCC CCTCCCCCAA CGACTACCAT 
AAAGTGGATG AAGAATTTGA GACTGTCTCT ACGCAGCTGC TCAAACGCAC CCAGGCCATG 
CTCAATAAAT ATCGGCTTTT GCTTCTGGAA GAGTCCAGGA GAGTCAGTCC TTCTGCGGAG 
ATGGTTATGA TCGACCGAAT GTTCATTCAG GAGGAGAAGA CCACCCTTGC CTTGGATAAG 
CAGCTTGCCA AGGAGAAGCC TGATGAGTAC GTGTCTTCCT CCCGCTCCCT TGGCTTCCCT 
GTCCCAGTGT CTTCCGAGGG CCACCGGCTC CCCAGCCATG GCCAGTCGTC TTCATCCTCC 
ACATCTGGAA CGTCTGCCCA GCCCCCTCCT CATCTGCCCA CCAAGCTAGT GATCCGGCAC 
GGTGGGGCCG GCGGCTCTCC CTCAGTGACC TGGGCCCGGG CATCCTCCTC CTTGTCATCC 
ACTTCCTCAT CCTCCTCCTC ATCCTCTGCT GCCTCATCCC TGGACGCAGA TGAGGACGGC 
CCCATGCCCA CCCGTAACCG GCCACCCATC AAGACCTATG AGGCCCGGAG CCGCATTGGT 
CTCAAACTCA AGATCAAACA AGAGGCGGGG CTCAGCAAGG TGGTGCACAA CACTGCACTG 
GATCCTGTGC ATCAGCCCTT GCCGGCTCCA ACCCCAGCGA AAGGGGCGGA GCCTCCGCCA 
CACCCAGCTC CGCCCCCACT CCCTCCTGCT ACCCAGGCGC AGATGAATGG CACTCTGGAC 
CATCCCCCAC CCGCAGTACG CAAACCCACG GTGCCTGCGT CCTGCCCACG TCTACCACTA 
CGCAAGACCT ACCGAGAAAA CATGGGCAAT CCTGGTGCCG CCGAGGGTGC ACAGGGACGG 
CCGCGGGGTG CGGGCAGCCC CACCCCACTG CCCACCAAGG TAGACGAAGC CACCAGTGGG 
CTGATCCGGG AGCTGGCAGC GGTGGAGGAT GAACTATATC AGCGGGTTCT GAAGGGCGGC 
CCACCACCCC CGGAGACTCC AGCCTCCGCT ACCAGCCAGG GCCCCACTGA ACCCAGTTGG 
GAAGCACCCG TGCTACCCCC AGCCAAACGA CGCAAGTCTG AGTCCCCGGA CGTGGACCAG 
GCCAGCTTCT CTAGTGACAG CCCGCAGGAT GATACACTTA CTGAGCATTT GCAGAGTGCC 
ATCGACAGCA TCCTTAACCT GCAGCAGGCC CCCGGCCGGA CACCCGCAGG CCCATACCCC 
CATACGGGGC CCACGCCTGG CACCCCCACA TCCCCAGCGC CCCTGCACAG GCCTGACGCC 
TTCCCACCCT CTAGTCACAA TGGTGGCCTC GGTGCCAGGA CGTTGAACAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicra ( XM_006539917.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539980.1 Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02393
Clone ID Related Accession (Same CDS sequence)	NM_001081418.1 , XM_006539917.3 , XM_006539917.4
Accession Version	XM_006539917.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	BRD4-interacting chromatin-remodeling complex-associated protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539917.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGGAGAGGAG CCAGCAGCCC CTCTCTACAG AGGGTCCCCA CCTCTCGGTG 
CCTGCCTCCG TCATAGTCAG CGCCCCGCCT CCTGCCCAAG ACCCAGCCCT GGCCACGCCC 
GTCACCAAAG GAGCTGGCCT CGGCGCTCAG ACCCCGGACA GCCGGGCTTC CCCAGCTCCG 
GCTCCCCAGA TCCCTGCAGC TGCTCCACTG AAAGCCCCTG GCCCCGCCTC CTCCCCCTCA 
CTACCTCACC AGGCCCCCCT GGGAGACAGT CCCCACATGC CCTCCCCACA CCCTGCCAGG 
CCCCCTTCCC GCCCACCCTC AAGACCCCAC TCACGCCCTC CATCCCAGCC CCAGAGCCTG 
ACCTGCCCAC CCTCTGAGCC CACCCTGCAC CCTTGCCCTC CACCCCAGGG TCCCCCAACT 
CTACCTGGCA TCTTTGTCAT CCAGAATCAA TTGGGCGCCC CACCACCAGC CAGCACCCCA 
GCCTCCACAG CCCCGGGCCC ACCCCAGCCT CCTCTGCGAC CCCCATCCCA GCCTCCAGAG 
GGCCCACTGC CCCCAGCCTC CCACCTCCCT CCTGCCTCCA CCCCCTCGGC CGTGGCCTCC 
TCCTCTGAGC CTTCTGCCAG GTTGCCGGTC CCCACACCCC CTGACTTCCA ACTCCAGTTC 
CCACCGAGCC AGGGACCCCA TAAGTCCCCT ACTCCGCCAC CAGCCCTCCA CATGGTCCCT 
GAGCCCACGG CACCCCCTCC TCCACCACCT CGGACCTTCC AGATGGTAAC CGCCCCCTTC 
CCAGCGTTGC CCCAGCCAAA AGCACTTCTG GAACGATTCC ACCAGGTGCC ATCTGGGATT 
ATTCTCCAGA ATAAGGCTGG GGGTACTCCC ACCACCCCAC AGACATCCAC CACCCTGGGG 
ACCCTCACCG GTCCTACTGC CTCTGTGCTA GTCAGTGGAC AGGCACCACC TGGGACTCCT 
GCCGCCTCTA GCCATGTCCC AGCCTCCACA CCTATGGCCA CCACAGGCCT CCCTCCTCTA 
CTTCCTGCCG AAAACAAAGC TTTTGCCAGC AACCTTCCAA CCCTGAGTGT GGCCAAAGCT 
ACCGTGTCTG GGCCAGGGAA GCCCCCAGCA ATTCAGTATG ACAGCAAGTT GTGTAGCTTG 
AAGAAACAGC CCCTACTGCA ACCCAGCAAA GAAGCCTGCT TCCTGGAGCA TCTGCACAAA 
CACCAGGGCT CTGTCCTGCA CCCCGATTAC AAGACAGCCT TCCCCTCCTT TGAGGACGCC 
CTCCATCGCC TCCTGCCCTA CCATGTCTAC CAAGGCGCCC TCCCCTCCCC CAACGACTAC 
CATAAAGTGG ATGAAGAATT TGAGACTGTC TCTACGCAGC TGCTCAAACG CACCCAGGCC 
ATGCTCAATA AATATCGGCT TTTGCTTCTG GAAGAGTCCA GGAGAGTCAG TCCTTCTGCG 
GAGATGGTTA TGATCGACCG AATGTTCATT CAGGAGGAGA AGACCACCCT TGCCTTGGAT 
AAGCAGCTTG CCAAGGAGAA GCCTGATGAG TACGTGTCTT CCTCCCGCTC CCTTGGCTTC 
CCTGTCCCAG TGTCTTCCGA GGGCCACCGG CTCCCCAGCC ATGGCCAGTC GTCTTCATCC 
TCCACATCTG GAACGTCTGC CCAGCCCCCT CCTCATCTGC CCACCAAGCT AGTGATCCGG 
CACGGTGGGG CCGGCGGCTC TCCCTCAGTG ACCTGGGCCC GGGCATCCTC CTCCTTGTCA 
TCCACTTCCT CATCCTCCTC CTCATCCTCT GCTGCCTCAT CCCTGGACGC AGATGAGGAC 
GGCCCCATGC CCACCCGTAA CCGGCCACCC ATCAAGACCT ATGAGGCCCG GAGCCGCATT 
GGTCTCAAAC TCAAGATCAA ACAAGAGGCG GGGCTCAGCA AGGTGGTGCA CAACACTGCA 
CTGGATCCTG TGCATCAGCC CTTGCCGGCT CCAACCCCAG CGAAAGGGGC GGAGCCTCCG 
CCACACCCAG CTCCGCCCCC ACTCCCTCCT GCTACCCAGG CGCAGATGAA TGGCACTCTG 
GACCATCCCC CACCCGCAGT ACGCAAACCC ACGGTGCCTG CGTCCTGCCC ACGTCTACCA 
CTACGCAAGA CCTACCGAGA AAACATGGGC AATCCTGGTG CCGCCGAGGG TGCACAGGGA 
CGGCCGCGGG GTGCGGGCAG CCCCACCCCA CTGCCCACCA AGGTAGACGA AGCCACCAGT 
GGGCTGATCC GGGAGCTGGC AGCGGTGGAG GATGAACTAT ATCAGCGGGT TCTGAAGGGC 
GGCCCACCAC CCCCGGAGAC TCCAGCCTCC GCTACCAGCC AGGGCCCCAC TGAACCCAGT 
TGGGAAGCAC CCGTGCTACC CCCAGCCAAA CGACGCAAGT CTGAGTCCCC GGACGTGGAC 
CAGGCCAGCT TCTCTAGTGA CAGCCCGCAG GATGATACAC TTACTGAGCA TTTGCAGAGT 
GCCATCGACA GCATCCTTAA CCTGCAGCAG GCCCCCGGCC GGACACCCGC AGGCCCATAC 
CCCCATACGG GGCCCACGCC TGGCACCCCC ACATCCCCAG CGCCCCTGCA CAGGCCTGAC 
GCCTTCCCAC CCTCTAGTCA CAATGGTGGC CTCGGTGCCA GGACGTTGAA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539980.1
CDS	358..5094
Translation	MDDEDGRCLLDVICDPQALNDFLHGSEKLDSDDLLDAPVEAQSAFYEGPGLHVQEAAANHLNPEPSQPAPSVDLDFLEDDILGSPAAGGGGGGGGAPDQPCDILQQSLQEANITEQTLEAEAELDLGPFQLPTLQPADNGAGATGAAGATAVTAGPQALFPGSADLLGLQAPPTVLTHQALVPPQDVVNKALSVQPFLQPVGLGNVTLQPISGLQGLPNGSPGNAAAATLGLTPIQVVGQPVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLPAAVTTTLNGNSVFAGTGAATAAASGAPSGQPLAVAPGLGTSPLVQAPSVILHRTPTPIQPKPTGVLPSKLYQLTPKPFPPTGATLTIQGEPGTLPQQPKAPQNLTFMATGKAGQNVVLSGFPAPALQANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTPAVQLPQSLSALPANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAAHILSAAPIQVGQPALFQMPVSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNTPDGLVQPSTPAATTGEATPVLAVQPATQVPPAVTTPLPMGLQQPQAQQPPQVPTPQAATQPQATPPQASPSLASSPEKIVLGQAPPAATTAILTQDSLQMFLPQERSQQPLSTEGPHLSVPASVIVSAPPPAQDPALATPVTKGAGLGAQTPDSRASPAPAPQIPAAAPLKAPGPASSPSLPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPTLPGIFVIQNQLGAPPPASTPASTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVASSSEPSARLPVPTPPDFQLQFPPSQGPHKSPTPPPALHMVPEPTAPPPPPPRTFQMVTAPFPALPQPKALLERFHQVPSGIILQNKAGGTPTTPQTSTTLGTLTGPTASVLVSGQAPPGTPAASSHVPASTPMATTGLPPLLPAENKAFASNLPTLSVAKATVSGPGKPPAIQYDSKLCSLKKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPNDYHKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALDKQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGQSSSSSTSGTSAQPPPHLPTKLVIRHGGAGGSPSVTWARASSSLSSTSSSSSSSSAASSLDADEDGPMPTRNRPPIKTYEARSRIGLKLKIKQEAGLSKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTLDHPPPAVRKPTVPASCPRLPLRKTYRENMGNPGAAEGAQGRPRGAGSPTPLPTKVDEATSGLIRELAAVEDELYQRVLKGGPPPPETPASATSQGPTEPSWEAPVLPPAKRRKSESPDVDQASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPHTGPTPGTPTSPAPLHRPDAFPPSSHNGGLGARTLNR

Target ORF information:

RefSeq Version	XM_006539917.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539917.4

ORF Insert Sequence:

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGGAGAGGAG CCAGCAGCCC CTCTCTACAG AGGGTCCCCA CCTCTCGGTG 
CCTGCCTCCG TCATAGTCAG CGCCCCGCCT CCTGCCCAAG ACCCAGCCCT GGCCACGCCC 
GTCACCAAAG GAGCTGGCCT CGGCGCTCAG ACCCCGGACA GCCGGGCTTC CCCAGCTCCG 
GCTCCCCAGA TCCCTGCAGC TGCTCCACTG AAAGCCCCTG GCCCCGCCTC CTCCCCCTCA 
CTACCTCACC AGGCCCCCCT GGGAGACAGT CCCCACATGC CCTCCCCACA CCCTGCCAGG 
CCCCCTTCCC GCCCACCCTC AAGACCCCAC TCACGCCCTC CATCCCAGCC CCAGAGCCTG 
ACCTGCCCAC CCTCTGAGCC CACCCTGCAC CCTTGCCCTC CACCCCAGGG TCCCCCAACT 
CTACCTGGCA TCTTTGTCAT CCAGAATCAA TTGGGCGCCC CACCACCAGC CAGCACCCCA 
GCCTCCACAG CCCCGGGCCC ACCCCAGCCT CCTCTGCGAC CCCCATCCCA GCCTCCAGAG 
GGCCCACTGC CCCCAGCCTC CCACCTCCCT CCTGCCTCCA CCCCCTCGGC CGTGGCCTCC 
TCCTCTGAGC CTTCTGCCAG GTTGCCGGTC CCCACACCCC CTGACTTCCA ACTCCAGTTC 
CCACCGAGCC AGGGACCCCA TAAGTCCCCT ACTCCGCCAC CAGCCCTCCA CATGGTCCCT 
GAGCCCACGG CACCCCCTCC TCCACCACCT CGGACCTTCC AGATGGTAAC CGCCCCCTTC 
CCAGCGTTGC CCCAGCCAAA AGCACTTCTG GAACGATTCC ACCAGGTGCC ATCTGGGATT 
ATTCTCCAGA ATAAGGCTGG GGGTACTCCC ACCACCCCAC AGACATCCAC CACCCTGGGG 
ACCCTCACCG GTCCTACTGC CTCTGTGCTA GTCAGTGGAC AGGCACCACC TGGGACTCCT 
GCCGCCTCTA GCCATGTCCC AGCCTCCACA CCTATGGCCA CCACAGGCCT CCCTCCTCTA 
CTTCCTGCCG AAAACAAAGC TTTTGCCAGC AACCTTCCAA CCCTGAGTGT GGCCAAAGCT 
ACCGTGTCTG GGCCAGGGAA GCCCCCAGCA ATTCAGTATG ACAGCAAGTT GTGTAGCTTG 
AAGAAACAGC CCCTACTGCA ACCCAGCAAA GAAGCCTGCT TCCTGGAGCA TCTGCACAAA 
CACCAGGGCT CTGTCCTGCA CCCCGATTAC AAGACAGCCT TCCCCTCCTT TGAGGACGCC 
CTCCATCGCC TCCTGCCCTA CCATGTCTAC CAAGGCGCCC TCCCCTCCCC CAACGACTAC 
CATAAAGTGG ATGAAGAATT TGAGACTGTC TCTACGCAGC TGCTCAAACG CACCCAGGCC 
ATGCTCAATA AATATCGGCT TTTGCTTCTG GAAGAGTCCA GGAGAGTCAG TCCTTCTGCG 
GAGATGGTTA TGATCGACCG AATGTTCATT CAGGAGGAGA AGACCACCCT TGCCTTGGAT 
AAGCAGCTTG CCAAGGAGAA GCCTGATGAG TACGTGTCTT CCTCCCGCTC CCTTGGCTTC 
CCTGTCCCAG TGTCTTCCGA GGGCCACCGG CTCCCCAGCC ATGGCCAGTC GTCTTCATCC 
TCCACATCTG GAACGTCTGC CCAGCCCCCT CCTCATCTGC CCACCAAGCT AGTGATCCGG 
CACGGTGGGG CCGGCGGCTC TCCCTCAGTG ACCTGGGCCC GGGCATCCTC CTCCTTGTCA 
TCCACTTCCT CATCCTCCTC CTCATCCTCT GCTGCCTCAT CCCTGGACGC AGATGAGGAC 
GGCCCCATGC CCACCCGTAA CCGGCCACCC ATCAAGACCT ATGAGGCCCG GAGCCGCATT 
GGTCTCAAAC TCAAGATCAA ACAAGAGGCG GGGCTCAGCA AGGTGGTGCA CAACACTGCA 
CTGGATCCTG TGCATCAGCC CTTGCCGGCT CCAACCCCAG CGAAAGGGGC GGAGCCTCCG 
CCACACCCAG CTCCGCCCCC ACTCCCTCCT GCTACCCAGG CGCAGATGAA TGGCACTCTG 
GACCATCCCC CACCCGCAGT ACGCAAACCC ACGGTGCCTG CGTCCTGCCC ACGTCTACCA 
CTACGCAAGA CCTACCGAGA AAACATGGGC AATCCTGGTG CCGCCGAGGG TGCACAGGGA 
CGGCCGCGGG GTGCGGGCAG CCCCACCCCA CTGCCCACCA AGGTAGACGA AGCCACCAGT 
GGGCTGATCC GGGAGCTGGC AGCGGTGGAG GATGAACTAT ATCAGCGGGT TCTGAAGGGC 
GGCCCACCAC CCCCGGAGAC TCCAGCCTCC GCTACCAGCC AGGGCCCCAC TGAACCCAGT 
TGGGAAGCAC CCGTGCTACC CCCAGCCAAA CGACGCAAGT CTGAGTCCCC GGACGTGGAC 
CAGGCCAGCT TCTCTAGTGA CAGCCCGCAG GATGATACAC TTACTGAGCA TTTGCAGAGT 
GCCATCGACA GCATCCTTAA CCTGCAGCAG GCCCCCGGCC GGACACCCGC AGGCCCATAC 
CCCCATACGG GGCCCACGCC TGGCACCCCC ACATCCCCAG CGCCCCTGCA CAGGCCTGAC 
GCCTTCCCAC CCTCTAGTCA CAATGGTGGC CTCGGTGCCA GGACGTTGAA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicra ( XM_006539918.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539981.1 Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X2, mRNA.

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CloneID	OMu38222
Clone ID Related Accession (Same CDS sequence)	XM_006539918.3 , XM_006539918.4
Accession Version	XM_006539918.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	BRD4-interacting chromatin-remodeling complex-associated protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539918.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTCCAT GTGCAGGAAG CTGCCGCCAA CCACCTAAAC 
CCTGAGCCCA GCCAGCCTGC CCCCAGCGTG GACCTGGACT TCCTAGAAGA TGATATCTTG 
GGCTCCCCTG CAGCAGGAGG AGGTGGAGGG GGCGGCGGGG CCCCAGACCA GCCCTGTGAC 
ATCCTTCAGC AGAGTCTTCA GGAGGCCAAC ATCACAGAAC AGACCCTGGA GGCTGAGGCT 
GAACTGGACC TGGGCCCCTT CCAGCTGCCC ACCCTACAGC CCGCTGACAA TGGGGCAGGT 
GCTACTGGAG CCGCAGGAGC CACGGCAGTG ACTGCAGGAC CCCAGGCTCT CTTCCCAGGC 
AGCGCGGATC TGCTGGGGCT GCAAGCCCCG CCCACTGTAC TGACCCACCA GGCCCTGGTG 
CCACCCCAGG ATGTGGTCAA CAAGGCCTTG AGCGTCCAGC CCTTCCTGCA GCCTGTGGGC 
CTGGGCAATG TGACCCTTCA GCCCATTTCA GGCCTCCAGG GCCTTCCCAA TGGCAGTCCT 
GGGAATGCTG CAGCAGCCAC CTTGGGTCTG ACACCTATTC AAGTGGTGGG CCAGCCCGTC 
ATGGCTCTCA ACCCACCCAC CTCCCAGCTC TTGGCAAAGC AGGTACCTGT CAGTGGCTAC 
CTGGCCTCAG CAGCTGGTCC TTCAGAGCCA GTGACACTGG CATCTGCCGG CGTGTCCCCC 
CAGGGAGCCG GCCTGGTCAT CCAGAAAAAT CTTCCAGCCG CAGTGACCAC CACACTCAAC 
GGGAACTCGG TGTTTGCCGG GACAGGGGCT GCCACTGCAG CAGCCAGTGG GGCACCCTCG 
GGACAGCCGC TGGCGGTGGC CCCGGGCCTT GGCACATCAC CACTGGTACA AGCACCCAGT 
GTGATTTTAC ACAGAACCCC TACGCCTATC CAGCCCAAGC CTACAGGGGT CCTGCCCTCC 
AAACTCTACC AGCTGACACC CAAGCCCTTT CCCCCTACCG GAGCCACCCT TACCATCCAG 
GGTGAACCAG GCACCTTGCC CCAGCAGCCT AAGGCCCCCC AGAACCTGAC TTTTATGGCC 
ACGGGCAAAG CTGGCCAGAA TGTGGTGCTG TCTGGCTTCC CGGCACCGGC TTTGCAGGCG 
AATGTGTTCA AGCAGCCACC AGTCACCACC ACGGGGACAG CCCCGCCACA GCCACCAGGG 
GCCCTCAGCA AACCCATGAG CGTCCACCTC CTCAATCAAG GCAGCAGCAT CGTGATCCCA 
GCCCAGCACA TGCTGCCTGG CCAGAACCAG TTCTTGCTGC CAGGCACCCC AGCCGTACAA 
CTCCCTCAGT CACTCTCTGC ACTGCCTGCC AACGTGGGAG GCCAGATCCT CACAGCTGCA 
GCACCACACG CAGGTGGACA GCTCATTGCC AACCCTATCC TCACCAACCA GAACCTGGCA 
GGCCCACTGA GTCTGGGCCC AGTGCTGGCA CCCCACTCTG GGGCACACAG CGCTGCACAC 
ATCCTCTCTG CAGCTCCCAT CCAGGTGGGC CAGCCTGCCC TCTTCCAGAT GCCTGTGTCA 
CTGGCCACTG GCAGCCTGCC TACTCAGAGC CAGCCGGCTC CCACTGGCCC CACAGCCACC 
ACCGTCCTCC AGGGCGTCAC CCTGCCTCCC AGTGCTGTGG CCATGCTTAA CACGCCTGAT 
GGGCTAGTGC AACCCTCCAC TCCAGCTGCC ACCACTGGGG AGGCCACACC AGTTCTGGCC 
GTTCAGCCTG CAACCCAGGT GCCCCCTGCT GTCACCACAC CACTGCCTAT GGGTCTCCAA 
CAGCCACAGG CACAGCAGCC TCCACAGGTC CCTACTCCAC AGGCGGCCAC CCAGCCTCAG 
GCCACCCCTC CTCAGGCCAG CCCAAGCCTG GCTTCCAGCC CAGAGAAGAT AGTCCTGGGG 
CAGGCGCCCC CTGCGGCCAC AACGGCCATC CTCACTCAGG ATTCCCTACA GATGTTCCTG 
CCCCAGGAGA GGAGCCAGCA GCCCCTCTCT ACAGAGGGTC CCCACCTCTC GGTGCCTGCC 
TCCGTCATAG TCAGCGCCCC GCCTCCTGCC CAAGACCCAG CCCTGGCCAC GCCCGTCACC 
AAAGGAGCTG GCCTCGGCGC TCAGACCCCG GACAGCCGGG CTTCCCCAGC TCCGGCTCCC 
CAGATCCCTG CAGCTGCTCC ACTGAAAGCC CCTGGCCCCG CCTCCTCCCC CTCACTACCT 
CACCAGGCCC CCCTGGGAGA CAGTCCCCAC ATGCCCTCCC CACACCCTGC CAGGCCCCCT 
TCCCGCCCAC CCTCAAGACC CCACTCACGC CCTCCATCCC AGCCCCAGAG CCTGACCTGC 
CCACCCTCTG AGCCCACCCT GCACCCTTGC CCTCCACCCC AGGGTCCCCC AACTCTACCT 
GGCATCTTTG TCATCCAGAA TCAATTGGGC GCCCCACCAC CAGCCAGCAC CCCAGCCTCC 
ACAGCCCCGG GCCCACCCCA GCCTCCTCTG CGACCCCCAT CCCAGCCTCC AGAGGGCCCA 
CTGCCCCCAG CCTCCCACCT CCCTCCTGCC TCCACCCCCT CGGCCGTGGC CTCCTCCTCT 
GAGCCTTCTG CCAGGTTGCC GGTCCCCACA CCCCCTGACT TCCAACTCCA GTTCCCACCG 
AGCCAGGGAC CCCATAAGTC CCCTACTCCG CCACCAGCCC TCCACATGGT CCCTGAGCCC 
ACGGCACCCC CTCCTCCACC ACCTCGGACC TTCCAGATGG TAACCGCCCC CTTCCCAGCG 
TTGCCCCAGC CAAAAGCACT TCTGGAACGA TTCCACCAGG TGCCATCTGG GATTATTCTC 
CAGAATAAGG CTGGGGGTAC TCCCACCACC CCACAGACAT CCACCACCCT GGGGACCCTC 
ACCGGTCCTA CTGCCTCTGT GCTAGTCAGT GGACAGGCAC CACCTGGGAC TCCTGCCGCC 
TCTAGCCATG TCCCAGCCTC CACACCTATG GCCACCACAG GCCTCCCTCC TCTACTTCCT 
GCCGAAAACA AAGCTTTTGC CAGCAACCTT CCAACCCTGA GTGTGGCCAA AGCTACCGTG 
TCTGGGCCAG GGAAGCCCCC AGCAATTCAG TATGACAGCA AGTTGTGTAG CTTGAAGAAA 
CAGCCCCTAC TGCAACCCAG CAAAGAAGCC TGCTTCCTGG AGCATCTGCA CAAACACCAG 
GGCTCTGTCC TGCACCCCGA TTACAAGACA GCCTTCCCCT CCTTTGAGGA CGCCCTCCAT 
CGCCTCCTGC CCTACCATGT CTACCAAGGC GCCCTCCCCT CCCCCAACGA CTACCATAAA 
GTGGATGAAG AATTTGAGAC TGTCTCTACG CAGCTGCTCA AACGCACCCA GGCCATGCTC 
AATAAATATC GGCTTTTGCT TCTGGAAGAG TCCAGGAGAG TCAGTCCTTC TGCGGAGATG 
GTTATGATCG ACCGAATGTT CATTCAGGAG GAGAAGACCA CCCTTGCCTT GGATAAGCAG 
CTTGCCAAGG AGAAGCCTGA TGAGTACGTG TCTTCCTCCC GCTCCCTTGG CTTCCCTGTC 
CCAGTGTCTT CCGAGGGCCA CCGGCTCCCC AGCCATGGCC AGTCGTCTTC ATCCTCCACA 
TCTGGAACGT CTGCCCAGCC CCCTCCTCAT CTGCCCACCA AGCTAGTGAT CCGGCACGGT 
GGGGCCGGCG GCTCTCCCTC AGTGACCTGG GCCCGGGCAT CCTCCTCCTT GTCATCCACT 
TCCTCATCCT CCTCCTCATC CTCTGCTGCC TCATCCCTGG ACGCAGATGA GGACGGCCCC 
ATGCCCACCC GTAACCGGCC ACCCATCAAG ACCTATGAGG CCCGGAGCCG CATTGGTCTC 
AAACTCAAGA TCAAACAAGA GGCGGGGCTC AGCAAGGTGG TGCACAACAC TGCACTGGAT 
CCTGTGCATC AGCCCTTGCC GGCTCCAACC CCAGCGAAAG GGGCGGAGCC TCCGCCACAC 
CCAGCTCCGC CCCCACTCCC TCCTGCTACC CAGGCGCAGA TGAATGGCAC TCTGGACCAT 
CCCCCACCCG CAGTACGCAA ACCCACGGTG CCTGCGTCCT GCCCACGTCT ACCACTACGC 
AAGACCTACC GAGAAAACAT GGGCAATCCT GGTGCCGCCG AGGGTGCACA GGGACGGCCG 
CGGGGTGCGG GCAGCCCCAC CCCACTGCCC ACCAAGGTAG ACGAAGCCAC CAGTGGGCTG 
ATCCGGGAGC TGGCAGCGGT GGAGGATGAA CTATATCAGC GGGTTCTGAA GGGCGGCCCA 
CCACCCCCGG AGACTCCAGC CTCCGCTACC AGCCAGGGCC CCACTGAACC CAGTTGGGAA 
GCACCCGTGC TACCCCCAGC CAAACGACGC AAGTCTGAGT CCCCGGACGT GGACCAGGCC 
AGCTTCTCTA GTGACAGCCC GCAGGATGAT ACACTTACTG AGCATTTGCA GAGTGCCATC 
GACAGCATCC TTAACCTGCA GCAGGCCCCC GGCCGGACAC CCGCAGGCCC ATACCCCCAT 
ACGGGGCCCA CGCCTGGCAC CCCCACATCC CCAGCGCCCC TGCACAGGCC TGACGCCTTC 
CCACCCTCTA GTCACAATGG TGGCCTCGGT GCCAGGACGT TGAACAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539981.1
CDS	358..5028
Translation	MDDEDGRCLLDVICDPQALNDFLHGSEKLHVQEAAANHLNPEPSQPAPSVDLDFLEDDILGSPAAGGGGGGGGAPDQPCDILQQSLQEANITEQTLEAEAELDLGPFQLPTLQPADNGAGATGAAGATAVTAGPQALFPGSADLLGLQAPPTVLTHQALVPPQDVVNKALSVQPFLQPVGLGNVTLQPISGLQGLPNGSPGNAAAATLGLTPIQVVGQPVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLPAAVTTTLNGNSVFAGTGAATAAASGAPSGQPLAVAPGLGTSPLVQAPSVILHRTPTPIQPKPTGVLPSKLYQLTPKPFPPTGATLTIQGEPGTLPQQPKAPQNLTFMATGKAGQNVVLSGFPAPALQANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTPAVQLPQSLSALPANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAAHILSAAPIQVGQPALFQMPVSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNTPDGLVQPSTPAATTGEATPVLAVQPATQVPPAVTTPLPMGLQQPQAQQPPQVPTPQAATQPQATPPQASPSLASSPEKIVLGQAPPAATTAILTQDSLQMFLPQERSQQPLSTEGPHLSVPASVIVSAPPPAQDPALATPVTKGAGLGAQTPDSRASPAPAPQIPAAAPLKAPGPASSPSLPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPTLPGIFVIQNQLGAPPPASTPASTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVASSSEPSARLPVPTPPDFQLQFPPSQGPHKSPTPPPALHMVPEPTAPPPPPPRTFQMVTAPFPALPQPKALLERFHQVPSGIILQNKAGGTPTTPQTSTTLGTLTGPTASVLVSGQAPPGTPAASSHVPASTPMATTGLPPLLPAENKAFASNLPTLSVAKATVSGPGKPPAIQYDSKLCSLKKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPNDYHKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALDKQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGQSSSSSTSGTSAQPPPHLPTKLVIRHGGAGGSPSVTWARASSSLSSTSSSSSSSSAASSLDADEDGPMPTRNRPPIKTYEARSRIGLKLKIKQEAGLSKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTLDHPPPAVRKPTVPASCPRLPLRKTYRENMGNPGAAEGAQGRPRGAGSPTPLPTKVDEATSGLIRELAAVEDELYQRVLKGGPPPPETPASATSQGPTEPSWEAPVLPPAKRRKSESPDVDQASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPHTGPTPGTPTSPAPLHRPDAFPPSSHNGGLGARTLNR

Target ORF information:

RefSeq Version	XM_006539918.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539918.4

ORF Insert Sequence:

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTCCAT GTGCAGGAAG CTGCCGCCAA CCACCTAAAC 
CCTGAGCCCA GCCAGCCTGC CCCCAGCGTG GACCTGGACT TCCTAGAAGA TGATATCTTG 
GGCTCCCCTG CAGCAGGAGG AGGTGGAGGG GGCGGCGGGG CCCCAGACCA GCCCTGTGAC 
ATCCTTCAGC AGAGTCTTCA GGAGGCCAAC ATCACAGAAC AGACCCTGGA GGCTGAGGCT 
GAACTGGACC TGGGCCCCTT CCAGCTGCCC ACCCTACAGC CCGCTGACAA TGGGGCAGGT 
GCTACTGGAG CCGCAGGAGC CACGGCAGTG ACTGCAGGAC CCCAGGCTCT CTTCCCAGGC 
AGCGCGGATC TGCTGGGGCT GCAAGCCCCG CCCACTGTAC TGACCCACCA GGCCCTGGTG 
CCACCCCAGG ATGTGGTCAA CAAGGCCTTG AGCGTCCAGC CCTTCCTGCA GCCTGTGGGC 
CTGGGCAATG TGACCCTTCA GCCCATTTCA GGCCTCCAGG GCCTTCCCAA TGGCAGTCCT 
GGGAATGCTG CAGCAGCCAC CTTGGGTCTG ACACCTATTC AAGTGGTGGG CCAGCCCGTC 
ATGGCTCTCA ACCCACCCAC CTCCCAGCTC TTGGCAAAGC AGGTACCTGT CAGTGGCTAC 
CTGGCCTCAG CAGCTGGTCC TTCAGAGCCA GTGACACTGG CATCTGCCGG CGTGTCCCCC 
CAGGGAGCCG GCCTGGTCAT CCAGAAAAAT CTTCCAGCCG CAGTGACCAC CACACTCAAC 
GGGAACTCGG TGTTTGCCGG GACAGGGGCT GCCACTGCAG CAGCCAGTGG GGCACCCTCG 
GGACAGCCGC TGGCGGTGGC CCCGGGCCTT GGCACATCAC CACTGGTACA AGCACCCAGT 
GTGATTTTAC ACAGAACCCC TACGCCTATC CAGCCCAAGC CTACAGGGGT CCTGCCCTCC 
AAACTCTACC AGCTGACACC CAAGCCCTTT CCCCCTACCG GAGCCACCCT TACCATCCAG 
GGTGAACCAG GCACCTTGCC CCAGCAGCCT AAGGCCCCCC AGAACCTGAC TTTTATGGCC 
ACGGGCAAAG CTGGCCAGAA TGTGGTGCTG TCTGGCTTCC CGGCACCGGC TTTGCAGGCG 
AATGTGTTCA AGCAGCCACC AGTCACCACC ACGGGGACAG CCCCGCCACA GCCACCAGGG 
GCCCTCAGCA AACCCATGAG CGTCCACCTC CTCAATCAAG GCAGCAGCAT CGTGATCCCA 
GCCCAGCACA TGCTGCCTGG CCAGAACCAG TTCTTGCTGC CAGGCACCCC AGCCGTACAA 
CTCCCTCAGT CACTCTCTGC ACTGCCTGCC AACGTGGGAG GCCAGATCCT CACAGCTGCA 
GCACCACACG CAGGTGGACA GCTCATTGCC AACCCTATCC TCACCAACCA GAACCTGGCA 
GGCCCACTGA GTCTGGGCCC AGTGCTGGCA CCCCACTCTG GGGCACACAG CGCTGCACAC 
ATCCTCTCTG CAGCTCCCAT CCAGGTGGGC CAGCCTGCCC TCTTCCAGAT GCCTGTGTCA 
CTGGCCACTG GCAGCCTGCC TACTCAGAGC CAGCCGGCTC CCACTGGCCC CACAGCCACC 
ACCGTCCTCC AGGGCGTCAC CCTGCCTCCC AGTGCTGTGG CCATGCTTAA CACGCCTGAT 
GGGCTAGTGC AACCCTCCAC TCCAGCTGCC ACCACTGGGG AGGCCACACC AGTTCTGGCC 
GTTCAGCCTG CAACCCAGGT GCCCCCTGCT GTCACCACAC CACTGCCTAT GGGTCTCCAA 
CAGCCACAGG CACAGCAGCC TCCACAGGTC CCTACTCCAC AGGCGGCCAC CCAGCCTCAG 
GCCACCCCTC CTCAGGCCAG CCCAAGCCTG GCTTCCAGCC CAGAGAAGAT AGTCCTGGGG 
CAGGCGCCCC CTGCGGCCAC AACGGCCATC CTCACTCAGG ATTCCCTACA GATGTTCCTG 
CCCCAGGAGA GGAGCCAGCA GCCCCTCTCT ACAGAGGGTC CCCACCTCTC GGTGCCTGCC 
TCCGTCATAG TCAGCGCCCC GCCTCCTGCC CAAGACCCAG CCCTGGCCAC GCCCGTCACC 
AAAGGAGCTG GCCTCGGCGC TCAGACCCCG GACAGCCGGG CTTCCCCAGC TCCGGCTCCC 
CAGATCCCTG CAGCTGCTCC ACTGAAAGCC CCTGGCCCCG CCTCCTCCCC CTCACTACCT 
CACCAGGCCC CCCTGGGAGA CAGTCCCCAC ATGCCCTCCC CACACCCTGC CAGGCCCCCT 
TCCCGCCCAC CCTCAAGACC CCACTCACGC CCTCCATCCC AGCCCCAGAG CCTGACCTGC 
CCACCCTCTG AGCCCACCCT GCACCCTTGC CCTCCACCCC AGGGTCCCCC AACTCTACCT 
GGCATCTTTG TCATCCAGAA TCAATTGGGC GCCCCACCAC CAGCCAGCAC CCCAGCCTCC 
ACAGCCCCGG GCCCACCCCA GCCTCCTCTG CGACCCCCAT CCCAGCCTCC AGAGGGCCCA 
CTGCCCCCAG CCTCCCACCT CCCTCCTGCC TCCACCCCCT CGGCCGTGGC CTCCTCCTCT 
GAGCCTTCTG CCAGGTTGCC GGTCCCCACA CCCCCTGACT TCCAACTCCA GTTCCCACCG 
AGCCAGGGAC CCCATAAGTC CCCTACTCCG CCACCAGCCC TCCACATGGT CCCTGAGCCC 
ACGGCACCCC CTCCTCCACC ACCTCGGACC TTCCAGATGG TAACCGCCCC CTTCCCAGCG 
TTGCCCCAGC CAAAAGCACT TCTGGAACGA TTCCACCAGG TGCCATCTGG GATTATTCTC 
CAGAATAAGG CTGGGGGTAC TCCCACCACC CCACAGACAT CCACCACCCT GGGGACCCTC 
ACCGGTCCTA CTGCCTCTGT GCTAGTCAGT GGACAGGCAC CACCTGGGAC TCCTGCCGCC 
TCTAGCCATG TCCCAGCCTC CACACCTATG GCCACCACAG GCCTCCCTCC TCTACTTCCT 
GCCGAAAACA AAGCTTTTGC CAGCAACCTT CCAACCCTGA GTGTGGCCAA AGCTACCGTG 
TCTGGGCCAG GGAAGCCCCC AGCAATTCAG TATGACAGCA AGTTGTGTAG CTTGAAGAAA 
CAGCCCCTAC TGCAACCCAG CAAAGAAGCC TGCTTCCTGG AGCATCTGCA CAAACACCAG 
GGCTCTGTCC TGCACCCCGA TTACAAGACA GCCTTCCCCT CCTTTGAGGA CGCCCTCCAT 
CGCCTCCTGC CCTACCATGT CTACCAAGGC GCCCTCCCCT CCCCCAACGA CTACCATAAA 
GTGGATGAAG AATTTGAGAC TGTCTCTACG CAGCTGCTCA AACGCACCCA GGCCATGCTC 
AATAAATATC GGCTTTTGCT TCTGGAAGAG TCCAGGAGAG TCAGTCCTTC TGCGGAGATG 
GTTATGATCG ACCGAATGTT CATTCAGGAG GAGAAGACCA CCCTTGCCTT GGATAAGCAG 
CTTGCCAAGG AGAAGCCTGA TGAGTACGTG TCTTCCTCCC GCTCCCTTGG CTTCCCTGTC 
CCAGTGTCTT CCGAGGGCCA CCGGCTCCCC AGCCATGGCC AGTCGTCTTC ATCCTCCACA 
TCTGGAACGT CTGCCCAGCC CCCTCCTCAT CTGCCCACCA AGCTAGTGAT CCGGCACGGT 
GGGGCCGGCG GCTCTCCCTC AGTGACCTGG GCCCGGGCAT CCTCCTCCTT GTCATCCACT 
TCCTCATCCT CCTCCTCATC CTCTGCTGCC TCATCCCTGG ACGCAGATGA GGACGGCCCC 
ATGCCCACCC GTAACCGGCC ACCCATCAAG ACCTATGAGG CCCGGAGCCG CATTGGTCTC 
AAACTCAAGA TCAAACAAGA GGCGGGGCTC AGCAAGGTGG TGCACAACAC TGCACTGGAT 
CCTGTGCATC AGCCCTTGCC GGCTCCAACC CCAGCGAAAG GGGCGGAGCC TCCGCCACAC 
CCAGCTCCGC CCCCACTCCC TCCTGCTACC CAGGCGCAGA TGAATGGCAC TCTGGACCAT 
CCCCCACCCG CAGTACGCAA ACCCACGGTG CCTGCGTCCT GCCCACGTCT ACCACTACGC 
AAGACCTACC GAGAAAACAT GGGCAATCCT GGTGCCGCCG AGGGTGCACA GGGACGGCCG 
CGGGGTGCGG GCAGCCCCAC CCCACTGCCC ACCAAGGTAG ACGAAGCCAC CAGTGGGCTG 
ATCCGGGAGC TGGCAGCGGT GGAGGATGAA CTATATCAGC GGGTTCTGAA GGGCGGCCCA 
CCACCCCCGG AGACTCCAGC CTCCGCTACC AGCCAGGGCC CCACTGAACC CAGTTGGGAA 
GCACCCGTGC TACCCCCAGC CAAACGACGC AAGTCTGAGT CCCCGGACGT GGACCAGGCC 
AGCTTCTCTA GTGACAGCCC GCAGGATGAT ACACTTACTG AGCATTTGCA GAGTGCCATC 
GACAGCATCC TTAACCTGCA GCAGGCCCCC GGCCGGACAC CCGCAGGCCC ATACCCCCAT 
ACGGGGCCCA CGCCTGGCAC CCCCACATCC CCAGCGCCCC TGCACAGGCC TGACGCCTTC 
CCACCCTCTA GTCACAATGG TGGCCTCGGT GCCAGGACGT TGAACAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicra ( XM_011250559.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248861.1 Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X3, mRNA.

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CloneID	OMu74158
Clone ID Related Accession (Same CDS sequence)	XM_011250559.2 , XM_011250559.3
Accession Version	XM_011250559.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	BRD4-interacting chromatin-remodeling complex-associated protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250559.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGATCCCTGC AGCTGCTCCA CTGAAAGCCC CTGGCCCCGC CTCCTCCCCC 
TCACTACCTC ACCAGGCCCC CCTGGGAGAC AGTCCCCACA TGCCCTCCCC ACACCCTGCC 
AGGCCCCCTT CCCGCCCACC CTCAAGACCC CACTCACGCC CTCCATCCCA GCCCCAGAGC 
CTGACCTGCC CACCCTCTGA GCCCACCCTG CACCCTTGCC CTCCACCCCA GGGTCCCCCA 
ACTCTACCTG GCATCTTTGT CATCCAGAAT CAATTGGGCG CCCCACCACC AGCCAGCACC 
CCAGCCTCCA CAGCCCCGGG CCCACCCCAG CCTCCTCTGC GACCCCCATC CCAGCCTCCA 
GAGGGCCCAC TGCCCCCAGC CTCCCACCTC CCTCCTGCCT CCACCCCCTC GGCCGTGGCC 
TCCTCCTCTG AGCCTTCTGC CAGGTTGCCG GTCCCCACAC CCCCTGACTT CCAACTCCAG 
TTCCCACCGA GCCAGGGACC CCATAAGTCC CCTACTCCGC CACCAGCCCT CCACATGGTC 
CCTGAGCCCA CGGCACCCCC TCCTCCACCA CCTCGGACCT TCCAGATGGT AACCGCCCCC 
TTCCCAGCGT TGCCCCAGCC AAAAGCACTT CTGGAACGAT TCCACCAGGT GCCATCTGGG 
ATTATTCTCC AGAATAAGGC TGGGGGTACT CCCACCACCC CACAGACATC CACCACCCTG 
GGGACCCTCA CCGGTCCTAC TGCCTCTGTG CTAGTCAGTG GACAGGCACC ACCTGGGACT 
CCTGCCGCCT CTAGCCATGT CCCAGCCTCC ACACCTATGG CCACCACAGG CCTCCCTCCT 
CTACTTCCTG CCGAAAACAA AGCTTTTGCC AGCAACCTTC CAACCCTGAG TGTGGCCAAA 
GCTACCGTGT CTGGGCCAGG GAAGCCCCCA GCAATTCAGT ATGACAGCAA GTTGTGTAGC 
TTGAAGAAAC AGCCCCTACT GCAACCCAGC AAAGAAGCCT GCTTCCTGGA GCATCTGCAC 
AAACACCAGG GCTCTGTCCT GCACCCCGAT TACAAGACAG CCTTCCCCTC CTTTGAGGAC 
GCCCTCCATC GCCTCCTGCC CTACCATGTC TACCAAGGCG CCCTCCCCTC CCCCAACGAC 
TACCATAAAG TGGATGAAGA ATTTGAGACT GTCTCTACGC AGCTGCTCAA ACGCACCCAG 
GCCATGCTCA ATAAATATCG GCTTTTGCTT CTGGAAGAGT CCAGGAGAGT CAGTCCTTCT 
GCGGAGATGG TTATGATCGA CCGAATGTTC ATTCAGGAGG AGAAGACCAC CCTTGCCTTG 
GATAAGCAGC TTGCCAAGGA GAAGCCTGAT GAGTACGTGT CTTCCTCCCG CTCCCTTGGC 
TTCCCTGTCC CAGTGTCTTC CGAGGGCCAC CGGCTCCCCA GCCATGGCCA GTCGTCTTCA 
TCCTCCACAT CTGGAACGTC TGCCCAGCCC CCTCCTCATC TGCCCACCAA GCTAGTGATC 
CGGCACGGTG GGGCCGGCGG CTCTCCCTCA GTGACCTGGG CCCGGGCATC CTCCTCCTTG 
TCATCCACTT CCTCATCCTC CTCCTCATCC TCTGCTGCCT CATCCCTGGA CGCAGATGAG 
GACGGCCCCA TGCCCACCCG TAACCGGCCA CCCATCAAGA CCTATGAGGC CCGGAGCCGC 
ATTGGTCTCA AACTCAAGAT CAAACAAGAG GCGGGGCTCA GCAAGGTGGT GCACAACACT 
GCACTGGATC CTGTGCATCA GCCCTTGCCG GCTCCAACCC CAGCGAAAGG GGCGGAGCCT 
CCGCCACACC CAGCTCCGCC CCCACTCCCT CCTGCTACCC AGGCGCAGAT GAATGGCACT 
CTGGACCATC CCCCACCCGC AGTACGCAAA CCCACGGTGC CTGCGTCCTG CCCACGTCTA 
CCACTACGCA AGACCTACCG AGAAAACATG GGCAATCCTG GTGCCGCCGA GGGTGCACAG 
GGACGGCCGC GGGGTGCGGG CAGCCCCACC CCACTGCCCA CCAAGGTAGA CGAAGCCACC 
AGTGGGCTGA TCCGGGAGCT GGCAGCGGTG GAGGATGAAC TATATCAGCG GGTTCTGAAG 
GGCGGCCCAC CACCCCCGGA GACTCCAGCC TCCGCTACCA GCCAGGGCCC CACTGAACCC 
AGTTGGGAAG CACCCGTGCT ACCCCCAGCC AAACGACGCA AGTCTGAGTC CCCGGACGTG 
GACCAGGCCA GCTTCTCTAG TGACAGCCCG CAGGATGATA CACTTACTGA GCATTTGCAG 
AGTGCCATCG ACAGCATCCT TAACCTGCAG CAGGCCCCCG GCCGGACACC CGCAGGCCCA 
TACCCCCATA CGGGGCCCAC GCCTGGCACC CCCACATCCC CAGCGCCCCT GCACAGGCCT 
GACGCCTTCC CACCCTCTAG TCACAATGGT GGCCTCGGTG CCAGGACGTT GAACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248861.1
CDS	358..4917
Translation	MDDEDGRCLLDVICDPQALNDFLHGSEKLDSDDLLDAPVEAQSAFYEGPGLHVQEAAANHLNPEPSQPAPSVDLDFLEDDILGSPAAGGGGGGGGAPDQPCDILQQSLQEANITEQTLEAEAELDLGPFQLPTLQPADNGAGATGAAGATAVTAGPQALFPGSADLLGLQAPPTVLTHQALVPPQDVVNKALSVQPFLQPVGLGNVTLQPISGLQGLPNGSPGNAAAATLGLTPIQVVGQPVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLPAAVTTTLNGNSVFAGTGAATAAASGAPSGQPLAVAPGLGTSPLVQAPSVILHRTPTPIQPKPTGVLPSKLYQLTPKPFPPTGATLTIQGEPGTLPQQPKAPQNLTFMATGKAGQNVVLSGFPAPALQANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTPAVQLPQSLSALPANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAAHILSAAPIQVGQPALFQMPVSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNTPDGLVQPSTPAATTGEATPVLAVQPATQVPPAVTTPLPMGLQQPQAQQPPQVPTPQAATQPQATPPQASPSLASSPEKIVLGQAPPAATTAILTQDSLQMFLPQIPAAAPLKAPGPASSPSLPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPTLPGIFVIQNQLGAPPPASTPASTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVASSSEPSARLPVPTPPDFQLQFPPSQGPHKSPTPPPALHMVPEPTAPPPPPPRTFQMVTAPFPALPQPKALLERFHQVPSGIILQNKAGGTPTTPQTSTTLGTLTGPTASVLVSGQAPPGTPAASSHVPASTPMATTGLPPLLPAENKAFASNLPTLSVAKATVSGPGKPPAIQYDSKLCSLKKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPNDYHKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALDKQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGQSSSSSTSGTSAQPPPHLPTKLVIRHGGAGGSPSVTWARASSSLSSTSSSSSSSSAASSLDADEDGPMPTRNRPPIKTYEARSRIGLKLKIKQEAGLSKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTLDHPPPAVRKPTVPASCPRLPLRKTYRENMGNPGAAEGAQGRPRGAGSPTPLPTKVDEATSGLIRELAAVEDELYQRVLKGGPPPPETPASATSQGPTEPSWEAPVLPPAKRRKSESPDVDQASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPHTGPTPGTPTSPAPLHRPDAFPPSSHNGGLGARTLNR

Target ORF information:

RefSeq Version	XM_011250559.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250559.3

ORF Insert Sequence:

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGATCCCTGC AGCTGCTCCA CTGAAAGCCC CTGGCCCCGC CTCCTCCCCC 
TCACTACCTC ACCAGGCCCC CCTGGGAGAC AGTCCCCACA TGCCCTCCCC ACACCCTGCC 
AGGCCCCCTT CCCGCCCACC CTCAAGACCC CACTCACGCC CTCCATCCCA GCCCCAGAGC 
CTGACCTGCC CACCCTCTGA GCCCACCCTG CACCCTTGCC CTCCACCCCA GGGTCCCCCA 
ACTCTACCTG GCATCTTTGT CATCCAGAAT CAATTGGGCG CCCCACCACC AGCCAGCACC 
CCAGCCTCCA CAGCCCCGGG CCCACCCCAG CCTCCTCTGC GACCCCCATC CCAGCCTCCA 
GAGGGCCCAC TGCCCCCAGC CTCCCACCTC CCTCCTGCCT CCACCCCCTC GGCCGTGGCC 
TCCTCCTCTG AGCCTTCTGC CAGGTTGCCG GTCCCCACAC CCCCTGACTT CCAACTCCAG 
TTCCCACCGA GCCAGGGACC CCATAAGTCC CCTACTCCGC CACCAGCCCT CCACATGGTC 
CCTGAGCCCA CGGCACCCCC TCCTCCACCA CCTCGGACCT TCCAGATGGT AACCGCCCCC 
TTCCCAGCGT TGCCCCAGCC AAAAGCACTT CTGGAACGAT TCCACCAGGT GCCATCTGGG 
ATTATTCTCC AGAATAAGGC TGGGGGTACT CCCACCACCC CACAGACATC CACCACCCTG 
GGGACCCTCA CCGGTCCTAC TGCCTCTGTG CTAGTCAGTG GACAGGCACC ACCTGGGACT 
CCTGCCGCCT CTAGCCATGT CCCAGCCTCC ACACCTATGG CCACCACAGG CCTCCCTCCT 
CTACTTCCTG CCGAAAACAA AGCTTTTGCC AGCAACCTTC CAACCCTGAG TGTGGCCAAA 
GCTACCGTGT CTGGGCCAGG GAAGCCCCCA GCAATTCAGT ATGACAGCAA GTTGTGTAGC 
TTGAAGAAAC AGCCCCTACT GCAACCCAGC AAAGAAGCCT GCTTCCTGGA GCATCTGCAC 
AAACACCAGG GCTCTGTCCT GCACCCCGAT TACAAGACAG CCTTCCCCTC CTTTGAGGAC 
GCCCTCCATC GCCTCCTGCC CTACCATGTC TACCAAGGCG CCCTCCCCTC CCCCAACGAC 
TACCATAAAG TGGATGAAGA ATTTGAGACT GTCTCTACGC AGCTGCTCAA ACGCACCCAG 
GCCATGCTCA ATAAATATCG GCTTTTGCTT CTGGAAGAGT CCAGGAGAGT CAGTCCTTCT 
GCGGAGATGG TTATGATCGA CCGAATGTTC ATTCAGGAGG AGAAGACCAC CCTTGCCTTG 
GATAAGCAGC TTGCCAAGGA GAAGCCTGAT GAGTACGTGT CTTCCTCCCG CTCCCTTGGC 
TTCCCTGTCC CAGTGTCTTC CGAGGGCCAC CGGCTCCCCA GCCATGGCCA GTCGTCTTCA 
TCCTCCACAT CTGGAACGTC TGCCCAGCCC CCTCCTCATC TGCCCACCAA GCTAGTGATC 
CGGCACGGTG GGGCCGGCGG CTCTCCCTCA GTGACCTGGG CCCGGGCATC CTCCTCCTTG 
TCATCCACTT CCTCATCCTC CTCCTCATCC TCTGCTGCCT CATCCCTGGA CGCAGATGAG 
GACGGCCCCA TGCCCACCCG TAACCGGCCA CCCATCAAGA CCTATGAGGC CCGGAGCCGC 
ATTGGTCTCA AACTCAAGAT CAAACAAGAG GCGGGGCTCA GCAAGGTGGT GCACAACACT 
GCACTGGATC CTGTGCATCA GCCCTTGCCG GCTCCAACCC CAGCGAAAGG GGCGGAGCCT 
CCGCCACACC CAGCTCCGCC CCCACTCCCT CCTGCTACCC AGGCGCAGAT GAATGGCACT 
CTGGACCATC CCCCACCCGC AGTACGCAAA CCCACGGTGC CTGCGTCCTG CCCACGTCTA 
CCACTACGCA AGACCTACCG AGAAAACATG GGCAATCCTG GTGCCGCCGA GGGTGCACAG 
GGACGGCCGC GGGGTGCGGG CAGCCCCACC CCACTGCCCA CCAAGGTAGA CGAAGCCACC 
AGTGGGCTGA TCCGGGAGCT GGCAGCGGTG GAGGATGAAC TATATCAGCG GGTTCTGAAG 
GGCGGCCCAC CACCCCCGGA GACTCCAGCC TCCGCTACCA GCCAGGGCCC CACTGAACCC 
AGTTGGGAAG CACCCGTGCT ACCCCCAGCC AAACGACGCA AGTCTGAGTC CCCGGACGTG 
GACCAGGCCA GCTTCTCTAG TGACAGCCCG CAGGATGATA CACTTACTGA GCATTTGCAG 
AGTGCCATCG ACAGCATCCT TAACCTGCAG CAGGCCCCCG GCCGGACACC CGCAGGCCCA 
TACCCCCATA CGGGGCCCAC GCCTGGCACC CCCACATCCC CAGCGCCCCT GCACAGGCCT 
GACGCCTTCC CACCCTCTAG TCACAATGGT GGCCTCGGTG CCAGGACGTT GAACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicra ( NM_001081418.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074887.1 Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), mRNA.

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CloneID	OMu02393
Clone ID Related Accession (Same CDS sequence)	NM_001081418.1 , XM_006539917.3 , XM_006539917.4
Accession Version	NM_001081418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	BRD4-interacting chromatin-remodeling complex-associated protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC148990.6. On Feb 6, 2007 this sequence version replaced XM_986680.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGGAGAGGAG CCAGCAGCCC CTCTCTACAG AGGGTCCCCA CCTCTCGGTG 
CCTGCCTCCG TCATAGTCAG CGCCCCGCCT CCTGCCCAAG ACCCAGCCCT GGCCACGCCC 
GTCACCAAAG GAGCTGGCCT CGGCGCTCAG ACCCCGGACA GCCGGGCTTC CCCAGCTCCG 
GCTCCCCAGA TCCCTGCAGC TGCTCCACTG AAAGCCCCTG GCCCCGCCTC CTCCCCCTCA 
CTACCTCACC AGGCCCCCCT GGGAGACAGT CCCCACATGC CCTCCCCACA CCCTGCCAGG 
CCCCCTTCCC GCCCACCCTC AAGACCCCAC TCACGCCCTC CATCCCAGCC CCAGAGCCTG 
ACCTGCCCAC CCTCTGAGCC CACCCTGCAC CCTTGCCCTC CACCCCAGGG TCCCCCAACT 
CTACCTGGCA TCTTTGTCAT CCAGAATCAA TTGGGCGCCC CACCACCAGC CAGCACCCCA 
GCCTCCACAG CCCCGGGCCC ACCCCAGCCT CCTCTGCGAC CCCCATCCCA GCCTCCAGAG 
GGCCCACTGC CCCCAGCCTC CCACCTCCCT CCTGCCTCCA CCCCCTCGGC CGTGGCCTCC 
TCCTCTGAGC CTTCTGCCAG GTTGCCGGTC CCCACACCCC CTGACTTCCA ACTCCAGTTC 
CCACCGAGCC AGGGACCCCA TAAGTCCCCT ACTCCGCCAC CAGCCCTCCA CATGGTCCCT 
GAGCCCACGG CACCCCCTCC TCCACCACCT CGGACCTTCC AGATGGTAAC CGCCCCCTTC 
CCAGCGTTGC CCCAGCCAAA AGCACTTCTG GAACGATTCC ACCAGGTGCC ATCTGGGATT 
ATTCTCCAGA ATAAGGCTGG GGGTACTCCC ACCACCCCAC AGACATCCAC CACCCTGGGG 
ACCCTCACCG GTCCTACTGC CTCTGTGCTA GTCAGTGGAC AGGCACCACC TGGGACTCCT 
GCCGCCTCTA GCCATGTCCC AGCCTCCACA CCTATGGCCA CCACAGGCCT CCCTCCTCTA 
CTTCCTGCCG AAAACAAAGC TTTTGCCAGC AACCTTCCAA CCCTGAGTGT GGCCAAAGCT 
ACCGTGTCTG GGCCAGGGAA GCCCCCAGCA ATTCAGTATG ACAGCAAGTT GTGTAGCTTG 
AAGAAACAGC CCCTACTGCA ACCCAGCAAA GAAGCCTGCT TCCTGGAGCA TCTGCACAAA 
CACCAGGGCT CTGTCCTGCA CCCCGATTAC AAGACAGCCT TCCCCTCCTT TGAGGACGCC 
CTCCATCGCC TCCTGCCCTA CCATGTCTAC CAAGGCGCCC TCCCCTCCCC CAACGACTAC 
CATAAAGTGG ATGAAGAATT TGAGACTGTC TCTACGCAGC TGCTCAAACG CACCCAGGCC 
ATGCTCAATA AATATCGGCT TTTGCTTCTG GAAGAGTCCA GGAGAGTCAG TCCTTCTGCG 
GAGATGGTTA TGATCGACCG AATGTTCATT CAGGAGGAGA AGACCACCCT TGCCTTGGAT 
AAGCAGCTTG CCAAGGAGAA GCCTGATGAG TACGTGTCTT CCTCCCGCTC CCTTGGCTTC 
CCTGTCCCAG TGTCTTCCGA GGGCCACCGG CTCCCCAGCC ATGGCCAGTC GTCTTCATCC 
TCCACATCTG GAACGTCTGC CCAGCCCCCT CCTCATCTGC CCACCAAGCT AGTGATCCGG 
CACGGTGGGG CCGGCGGCTC TCCCTCAGTG ACCTGGGCCC GGGCATCCTC CTCCTTGTCA 
TCCACTTCCT CATCCTCCTC CTCATCCTCT GCTGCCTCAT CCCTGGACGC AGATGAGGAC 
GGCCCCATGC CCACCCGTAA CCGGCCACCC ATCAAGACCT ATGAGGCCCG GAGCCGCATT 
GGTCTCAAAC TCAAGATCAA ACAAGAGGCG GGGCTCAGCA AGGTGGTGCA CAACACTGCA 
CTGGATCCTG TGCATCAGCC CTTGCCGGCT CCAACCCCAG CGAAAGGGGC GGAGCCTCCG 
CCACACCCAG CTCCGCCCCC ACTCCCTCCT GCTACCCAGG CGCAGATGAA TGGCACTCTG 
GACCATCCCC CACCCGCAGT ACGCAAACCC ACGGTGCCTG CGTCCTGCCC ACGTCTACCA 
CTACGCAAGA CCTACCGAGA AAACATGGGC AATCCTGGTG CCGCCGAGGG TGCACAGGGA 
CGGCCGCGGG GTGCGGGCAG CCCCACCCCA CTGCCCACCA AGGTAGACGA AGCCACCAGT 
GGGCTGATCC GGGAGCTGGC AGCGGTGGAG GATGAACTAT ATCAGCGGGT TCTGAAGGGC 
GGCCCACCAC CCCCGGAGAC TCCAGCCTCC GCTACCAGCC AGGGCCCCAC TGAACCCAGT 
TGGGAAGCAC CCGTGCTACC CCCAGCCAAA CGACGCAAGT CTGAGTCCCC GGACGTGGAC 
CAGGCCAGCT TCTCTAGTGA CAGCCCGCAG GATGATACAC TTACTGAGCA TTTGCAGAGT 
GCCATCGACA GCATCCTTAA CCTGCAGCAG GCCCCCGGCC GGACACCCGC AGGCCCATAC 
CCCCATACGG GGCCCACGCC TGGCACCCCC ACATCCCCAG CGCCCCTGCA CAGGCCTGAC 
GCCTTCCCAC CCTCTAGTCA CAATGGTGGC CTCGGTGCCA GGACGTTGAA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074887.1
CDS	108..4844
Translation	MDDEDGRCLLDVICDPQALNDFLHGSEKLDSDDLLDAPVEAQSAFYEGPGLHVQEAAANHLNPEPSQPAPSVDLDFLEDDILGSPAAGGGGGGGGAPDQPCDILQQSLQEANITEQTLEAEAELDLGPFQLPTLQPADNGAGATGAAGATAVTAGPQALFPGSADLLGLQAPPTVLTHQALVPPQDVVNKALSVQPFLQPVGLGNVTLQPISGLQGLPNGSPGNAAAATLGLTPIQVVGQPVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLPAAVTTTLNGNSVFAGTGAATAAASGAPSGQPLAVAPGLGTSPLVQAPSVILHRTPTPIQPKPTGVLPSKLYQLTPKPFPPTGATLTIQGEPGTLPQQPKAPQNLTFMATGKAGQNVVLSGFPAPALQANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTPAVQLPQSLSALPANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAAHILSAAPIQVGQPALFQMPVSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNTPDGLVQPSTPAATTGEATPVLAVQPATQVPPAVTTPLPMGLQQPQAQQPPQVPTPQAATQPQATPPQASPSLASSPEKIVLGQAPPAATTAILTQDSLQMFLPQERSQQPLSTEGPHLSVPASVIVSAPPPAQDPALATPVTKGAGLGAQTPDSRASPAPAPQIPAAAPLKAPGPASSPSLPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPTLPGIFVIQNQLGAPPPASTPASTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVASSSEPSARLPVPTPPDFQLQFPPSQGPHKSPTPPPALHMVPEPTAPPPPPPRTFQMVTAPFPALPQPKALLERFHQVPSGIILQNKAGGTPTTPQTSTTLGTLTGPTASVLVSGQAPPGTPAASSHVPASTPMATTGLPPLLPAENKAFASNLPTLSVAKATVSGPGKPPAIQYDSKLCSLKKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPNDYHKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALDKQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGQSSSSSTSGTSAQPPPHLPTKLVIRHGGAGGSPSVTWARASSSLSSTSSSSSSSSAASSLDADEDGPMPTRNRPPIKTYEARSRIGLKLKIKQEAGLSKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTLDHPPPAVRKPTVPASCPRLPLRKTYRENMGNPGAAEGAQGRPRGAGSPTPLPTKVDEATSGLIRELAAVEDELYQRVLKGGPPPPETPASATSQGPTEPSWEAPVLPPAKRRKSESPDVDQASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPHTGPTPGTPTSPAPLHRPDAFPPSSHNGGLGARTLNR

Target ORF information:

RefSeq Version	NM_001081418.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus BRD4 interacting chromatin remodeling complex associated protein (Bicra), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081418.1

ORF Insert Sequence:

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGGAGAGGAG CCAGCAGCCC CTCTCTACAG AGGGTCCCCA CCTCTCGGTG 
CCTGCCTCCG TCATAGTCAG CGCCCCGCCT CCTGCCCAAG ACCCAGCCCT GGCCACGCCC 
GTCACCAAAG GAGCTGGCCT CGGCGCTCAG ACCCCGGACA GCCGGGCTTC CCCAGCTCCG 
GCTCCCCAGA TCCCTGCAGC TGCTCCACTG AAAGCCCCTG GCCCCGCCTC CTCCCCCTCA 
CTACCTCACC AGGCCCCCCT GGGAGACAGT CCCCACATGC CCTCCCCACA CCCTGCCAGG 
CCCCCTTCCC GCCCACCCTC AAGACCCCAC TCACGCCCTC CATCCCAGCC CCAGAGCCTG 
ACCTGCCCAC CCTCTGAGCC CACCCTGCAC CCTTGCCCTC CACCCCAGGG TCCCCCAACT 
CTACCTGGCA TCTTTGTCAT CCAGAATCAA TTGGGCGCCC CACCACCAGC CAGCACCCCA 
GCCTCCACAG CCCCGGGCCC ACCCCAGCCT CCTCTGCGAC CCCCATCCCA GCCTCCAGAG 
GGCCCACTGC CCCCAGCCTC CCACCTCCCT CCTGCCTCCA CCCCCTCGGC CGTGGCCTCC 
TCCTCTGAGC CTTCTGCCAG GTTGCCGGTC CCCACACCCC CTGACTTCCA ACTCCAGTTC 
CCACCGAGCC AGGGACCCCA TAAGTCCCCT ACTCCGCCAC CAGCCCTCCA CATGGTCCCT 
GAGCCCACGG CACCCCCTCC TCCACCACCT CGGACCTTCC AGATGGTAAC CGCCCCCTTC 
CCAGCGTTGC CCCAGCCAAA AGCACTTCTG GAACGATTCC ACCAGGTGCC ATCTGGGATT 
ATTCTCCAGA ATAAGGCTGG GGGTACTCCC ACCACCCCAC AGACATCCAC CACCCTGGGG 
ACCCTCACCG GTCCTACTGC CTCTGTGCTA GTCAGTGGAC AGGCACCACC TGGGACTCCT 
GCCGCCTCTA GCCATGTCCC AGCCTCCACA CCTATGGCCA CCACAGGCCT CCCTCCTCTA 
CTTCCTGCCG AAAACAAAGC TTTTGCCAGC AACCTTCCAA CCCTGAGTGT GGCCAAAGCT 
ACCGTGTCTG GGCCAGGGAA GCCCCCAGCA ATTCAGTATG ACAGCAAGTT GTGTAGCTTG 
AAGAAACAGC CCCTACTGCA ACCCAGCAAA GAAGCCTGCT TCCTGGAGCA TCTGCACAAA 
CACCAGGGCT CTGTCCTGCA CCCCGATTAC AAGACAGCCT TCCCCTCCTT TGAGGACGCC 
CTCCATCGCC TCCTGCCCTA CCATGTCTAC CAAGGCGCCC TCCCCTCCCC CAACGACTAC 
CATAAAGTGG ATGAAGAATT TGAGACTGTC TCTACGCAGC TGCTCAAACG CACCCAGGCC 
ATGCTCAATA AATATCGGCT TTTGCTTCTG GAAGAGTCCA GGAGAGTCAG TCCTTCTGCG 
GAGATGGTTA TGATCGACCG AATGTTCATT CAGGAGGAGA AGACCACCCT TGCCTTGGAT 
AAGCAGCTTG CCAAGGAGAA GCCTGATGAG TACGTGTCTT CCTCCCGCTC CCTTGGCTTC 
CCTGTCCCAG TGTCTTCCGA GGGCCACCGG CTCCCCAGCC ATGGCCAGTC GTCTTCATCC 
TCCACATCTG GAACGTCTGC CCAGCCCCCT CCTCATCTGC CCACCAAGCT AGTGATCCGG 
CACGGTGGGG CCGGCGGCTC TCCCTCAGTG ACCTGGGCCC GGGCATCCTC CTCCTTGTCA 
TCCACTTCCT CATCCTCCTC CTCATCCTCT GCTGCCTCAT CCCTGGACGC AGATGAGGAC 
GGCCCCATGC CCACCCGTAA CCGGCCACCC ATCAAGACCT ATGAGGCCCG GAGCCGCATT 
GGTCTCAAAC TCAAGATCAA ACAAGAGGCG GGGCTCAGCA AGGTGGTGCA CAACACTGCA 
CTGGATCCTG TGCATCAGCC CTTGCCGGCT CCAACCCCAG CGAAAGGGGC GGAGCCTCCG 
CCACACCCAG CTCCGCCCCC ACTCCCTCCT GCTACCCAGG CGCAGATGAA TGGCACTCTG 
GACCATCCCC CACCCGCAGT ACGCAAACCC ACGGTGCCTG CGTCCTGCCC ACGTCTACCA 
CTACGCAAGA CCTACCGAGA AAACATGGGC AATCCTGGTG CCGCCGAGGG TGCACAGGGA 
CGGCCGCGGG GTGCGGGCAG CCCCACCCCA CTGCCCACCA AGGTAGACGA AGCCACCAGT 
GGGCTGATCC GGGAGCTGGC AGCGGTGGAG GATGAACTAT ATCAGCGGGT TCTGAAGGGC 
GGCCCACCAC CCCCGGAGAC TCCAGCCTCC GCTACCAGCC AGGGCCCCAC TGAACCCAGT 
TGGGAAGCAC CCGTGCTACC CCCAGCCAAA CGACGCAAGT CTGAGTCCCC GGACGTGGAC 
CAGGCCAGCT TCTCTAGTGA CAGCCCGCAG GATGATACAC TTACTGAGCA TTTGCAGAGT 
GCCATCGACA GCATCCTTAA CCTGCAGCAG GCCCCCGGCC GGACACCCGC AGGCCCATAC 
CCCCATACGG GGCCCACGCC TGGCACCCCC ACATCCCCAG CGCCCCTGCA CAGGCCTGAC 
GCCTTCCCAC CCTCTAGTCA CAATGGTGGC CTCGGTGCCA GGACGTTGAA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicra ( XM_006539917.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539980.1 Mus musculus glioma tumor suppressor candidate region gene 1 (Gltscr1), transcript variant X1, mRNA.

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CloneID	OMu02393
Clone ID Related Accession (Same CDS sequence)	NM_001081418.1 , XM_006539917.3 , XM_006539917.4
Accession Version	XM_006539917.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGGAGAGGAG CCAGCAGCCC CTCTCTACAG AGGGTCCCCA CCTCTCGGTG 
CCTGCCTCCG TCATAGTCAG CGCCCCGCCT CCTGCCCAAG ACCCAGCCCT GGCCACGCCC 
GTCACCAAAG GAGCTGGCCT CGGCGCTCAG ACCCCGGACA GCCGGGCTTC CCCAGCTCCG 
GCTCCCCAGA TCCCTGCAGC TGCTCCACTG AAAGCCCCTG GCCCCGCCTC CTCCCCCTCA 
CTACCTCACC AGGCCCCCCT GGGAGACAGT CCCCACATGC CCTCCCCACA CCCTGCCAGG 
CCCCCTTCCC GCCCACCCTC AAGACCCCAC TCACGCCCTC CATCCCAGCC CCAGAGCCTG 
ACCTGCCCAC CCTCTGAGCC CACCCTGCAC CCTTGCCCTC CACCCCAGGG TCCCCCAACT 
CTACCTGGCA TCTTTGTCAT CCAGAATCAA TTGGGCGCCC CACCACCAGC CAGCACCCCA 
GCCTCCACAG CCCCGGGCCC ACCCCAGCCT CCTCTGCGAC CCCCATCCCA GCCTCCAGAG 
GGCCCACTGC CCCCAGCCTC CCACCTCCCT CCTGCCTCCA CCCCCTCGGC CGTGGCCTCC 
TCCTCTGAGC CTTCTGCCAG GTTGCCGGTC CCCACACCCC CTGACTTCCA ACTCCAGTTC 
CCACCGAGCC AGGGACCCCA TAAGTCCCCT ACTCCGCCAC CAGCCCTCCA CATGGTCCCT 
GAGCCCACGG CACCCCCTCC TCCACCACCT CGGACCTTCC AGATGGTAAC CGCCCCCTTC 
CCAGCGTTGC CCCAGCCAAA AGCACTTCTG GAACGATTCC ACCAGGTGCC ATCTGGGATT 
ATTCTCCAGA ATAAGGCTGG GGGTACTCCC ACCACCCCAC AGACATCCAC CACCCTGGGG 
ACCCTCACCG GTCCTACTGC CTCTGTGCTA GTCAGTGGAC AGGCACCACC TGGGACTCCT 
GCCGCCTCTA GCCATGTCCC AGCCTCCACA CCTATGGCCA CCACAGGCCT CCCTCCTCTA 
CTTCCTGCCG AAAACAAAGC TTTTGCCAGC AACCTTCCAA CCCTGAGTGT GGCCAAAGCT 
ACCGTGTCTG GGCCAGGGAA GCCCCCAGCA ATTCAGTATG ACAGCAAGTT GTGTAGCTTG 
AAGAAACAGC CCCTACTGCA ACCCAGCAAA GAAGCCTGCT TCCTGGAGCA TCTGCACAAA 
CACCAGGGCT CTGTCCTGCA CCCCGATTAC AAGACAGCCT TCCCCTCCTT TGAGGACGCC 
CTCCATCGCC TCCTGCCCTA CCATGTCTAC CAAGGCGCCC TCCCCTCCCC CAACGACTAC 
CATAAAGTGG ATGAAGAATT TGAGACTGTC TCTACGCAGC TGCTCAAACG CACCCAGGCC 
ATGCTCAATA AATATCGGCT TTTGCTTCTG GAAGAGTCCA GGAGAGTCAG TCCTTCTGCG 
GAGATGGTTA TGATCGACCG AATGTTCATT CAGGAGGAGA AGACCACCCT TGCCTTGGAT 
AAGCAGCTTG CCAAGGAGAA GCCTGATGAG TACGTGTCTT CCTCCCGCTC CCTTGGCTTC 
CCTGTCCCAG TGTCTTCCGA GGGCCACCGG CTCCCCAGCC ATGGCCAGTC GTCTTCATCC 
TCCACATCTG GAACGTCTGC CCAGCCCCCT CCTCATCTGC CCACCAAGCT AGTGATCCGG 
CACGGTGGGG CCGGCGGCTC TCCCTCAGTG ACCTGGGCCC GGGCATCCTC CTCCTTGTCA 
TCCACTTCCT CATCCTCCTC CTCATCCTCT GCTGCCTCAT CCCTGGACGC AGATGAGGAC 
GGCCCCATGC CCACCCGTAA CCGGCCACCC ATCAAGACCT ATGAGGCCCG GAGCCGCATT 
GGTCTCAAAC TCAAGATCAA ACAAGAGGCG GGGCTCAGCA AGGTGGTGCA CAACACTGCA 
CTGGATCCTG TGCATCAGCC CTTGCCGGCT CCAACCCCAG CGAAAGGGGC GGAGCCTCCG 
CCACACCCAG CTCCGCCCCC ACTCCCTCCT GCTACCCAGG CGCAGATGAA TGGCACTCTG 
GACCATCCCC CACCCGCAGT ACGCAAACCC ACGGTGCCTG CGTCCTGCCC ACGTCTACCA 
CTACGCAAGA CCTACCGAGA AAACATGGGC AATCCTGGTG CCGCCGAGGG TGCACAGGGA 
CGGCCGCGGG GTGCGGGCAG CCCCACCCCA CTGCCCACCA AGGTAGACGA AGCCACCAGT 
GGGCTGATCC GGGAGCTGGC AGCGGTGGAG GATGAACTAT ATCAGCGGGT TCTGAAGGGC 
GGCCCACCAC CCCCGGAGAC TCCAGCCTCC GCTACCAGCC AGGGCCCCAC TGAACCCAGT 
TGGGAAGCAC CCGTGCTACC CCCAGCCAAA CGACGCAAGT CTGAGTCCCC GGACGTGGAC 
CAGGCCAGCT TCTCTAGTGA CAGCCCGCAG GATGATACAC TTACTGAGCA TTTGCAGAGT 
GCCATCGACA GCATCCTTAA CCTGCAGCAG GCCCCCGGCC GGACACCCGC AGGCCCATAC 
CCCCATACGG GGCCCACGCC TGGCACCCCC ACATCCCCAG CGCCCCTGCA CAGGCCTGAC 
GCCTTCCCAC CCTCTAGTCA CAATGGTGGC CTCGGTGCCA GGACGTTGAA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539980.1
CDS	297..5033
Translation	MDDEDGRCLLDVICDPQALNDFLHGSEKLDSDDLLDAPVEAQSAFYEGPGLHVQEAAANHLNPEPSQPAPSVDLDFLEDDILGSPAAGGGGGGGGAPDQPCDILQQSLQEANITEQTLEAEAELDLGPFQLPTLQPADNGAGATGAAGATAVTAGPQALFPGSADLLGLQAPPTVLTHQALVPPQDVVNKALSVQPFLQPVGLGNVTLQPISGLQGLPNGSPGNAAAATLGLTPIQVVGQPVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLPAAVTTTLNGNSVFAGTGAATAAASGAPSGQPLAVAPGLGTSPLVQAPSVILHRTPTPIQPKPTGVLPSKLYQLTPKPFPPTGATLTIQGEPGTLPQQPKAPQNLTFMATGKAGQNVVLSGFPAPALQANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTPAVQLPQSLSALPANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAAHILSAAPIQVGQPALFQMPVSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNTPDGLVQPSTPAATTGEATPVLAVQPATQVPPAVTTPLPMGLQQPQAQQPPQVPTPQAATQPQATPPQASPSLASSPEKIVLGQAPPAATTAILTQDSLQMFLPQERSQQPLSTEGPHLSVPASVIVSAPPPAQDPALATPVTKGAGLGAQTPDSRASPAPAPQIPAAAPLKAPGPASSPSLPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPTLPGIFVIQNQLGAPPPASTPASTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVASSSEPSARLPVPTPPDFQLQFPPSQGPHKSPTPPPALHMVPEPTAPPPPPPRTFQMVTAPFPALPQPKALLERFHQVPSGIILQNKAGGTPTTPQTSTTLGTLTGPTASVLVSGQAPPGTPAASSHVPASTPMATTGLPPLLPAENKAFASNLPTLSVAKATVSGPGKPPAIQYDSKLCSLKKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPNDYHKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALDKQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGQSSSSSTSGTSAQPPPHLPTKLVIRHGGAGGSPSVTWARASSSLSSTSSSSSSSSAASSLDADEDGPMPTRNRPPIKTYEARSRIGLKLKIKQEAGLSKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTLDHPPPAVRKPTVPASCPRLPLRKTYRENMGNPGAAEGAQGRPRGAGSPTPLPTKVDEATSGLIRELAAVEDELYQRVLKGGPPPPETPASATSQGPTEPSWEAPVLPPAKRRKSESPDVDQASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPHTGPTPGTPTSPAPLHRPDAFPPSSHNGGLGARTLNR

Target ORF information:

RefSeq Version	XM_006539917.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glioma tumor suppressor candidate region gene 1 (Gltscr1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539917.3

ORF Insert Sequence:

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGGAGAGGAG CCAGCAGCCC CTCTCTACAG AGGGTCCCCA CCTCTCGGTG 
CCTGCCTCCG TCATAGTCAG CGCCCCGCCT CCTGCCCAAG ACCCAGCCCT GGCCACGCCC 
GTCACCAAAG GAGCTGGCCT CGGCGCTCAG ACCCCGGACA GCCGGGCTTC CCCAGCTCCG 
GCTCCCCAGA TCCCTGCAGC TGCTCCACTG AAAGCCCCTG GCCCCGCCTC CTCCCCCTCA 
CTACCTCACC AGGCCCCCCT GGGAGACAGT CCCCACATGC CCTCCCCACA CCCTGCCAGG 
CCCCCTTCCC GCCCACCCTC AAGACCCCAC TCACGCCCTC CATCCCAGCC CCAGAGCCTG 
ACCTGCCCAC CCTCTGAGCC CACCCTGCAC CCTTGCCCTC CACCCCAGGG TCCCCCAACT 
CTACCTGGCA TCTTTGTCAT CCAGAATCAA TTGGGCGCCC CACCACCAGC CAGCACCCCA 
GCCTCCACAG CCCCGGGCCC ACCCCAGCCT CCTCTGCGAC CCCCATCCCA GCCTCCAGAG 
GGCCCACTGC CCCCAGCCTC CCACCTCCCT CCTGCCTCCA CCCCCTCGGC CGTGGCCTCC 
TCCTCTGAGC CTTCTGCCAG GTTGCCGGTC CCCACACCCC CTGACTTCCA ACTCCAGTTC 
CCACCGAGCC AGGGACCCCA TAAGTCCCCT ACTCCGCCAC CAGCCCTCCA CATGGTCCCT 
GAGCCCACGG CACCCCCTCC TCCACCACCT CGGACCTTCC AGATGGTAAC CGCCCCCTTC 
CCAGCGTTGC CCCAGCCAAA AGCACTTCTG GAACGATTCC ACCAGGTGCC ATCTGGGATT 
ATTCTCCAGA ATAAGGCTGG GGGTACTCCC ACCACCCCAC AGACATCCAC CACCCTGGGG 
ACCCTCACCG GTCCTACTGC CTCTGTGCTA GTCAGTGGAC AGGCACCACC TGGGACTCCT 
GCCGCCTCTA GCCATGTCCC AGCCTCCACA CCTATGGCCA CCACAGGCCT CCCTCCTCTA 
CTTCCTGCCG AAAACAAAGC TTTTGCCAGC AACCTTCCAA CCCTGAGTGT GGCCAAAGCT 
ACCGTGTCTG GGCCAGGGAA GCCCCCAGCA ATTCAGTATG ACAGCAAGTT GTGTAGCTTG 
AAGAAACAGC CCCTACTGCA ACCCAGCAAA GAAGCCTGCT TCCTGGAGCA TCTGCACAAA 
CACCAGGGCT CTGTCCTGCA CCCCGATTAC AAGACAGCCT TCCCCTCCTT TGAGGACGCC 
CTCCATCGCC TCCTGCCCTA CCATGTCTAC CAAGGCGCCC TCCCCTCCCC CAACGACTAC 
CATAAAGTGG ATGAAGAATT TGAGACTGTC TCTACGCAGC TGCTCAAACG CACCCAGGCC 
ATGCTCAATA AATATCGGCT TTTGCTTCTG GAAGAGTCCA GGAGAGTCAG TCCTTCTGCG 
GAGATGGTTA TGATCGACCG AATGTTCATT CAGGAGGAGA AGACCACCCT TGCCTTGGAT 
AAGCAGCTTG CCAAGGAGAA GCCTGATGAG TACGTGTCTT CCTCCCGCTC CCTTGGCTTC 
CCTGTCCCAG TGTCTTCCGA GGGCCACCGG CTCCCCAGCC ATGGCCAGTC GTCTTCATCC 
TCCACATCTG GAACGTCTGC CCAGCCCCCT CCTCATCTGC CCACCAAGCT AGTGATCCGG 
CACGGTGGGG CCGGCGGCTC TCCCTCAGTG ACCTGGGCCC GGGCATCCTC CTCCTTGTCA 
TCCACTTCCT CATCCTCCTC CTCATCCTCT GCTGCCTCAT CCCTGGACGC AGATGAGGAC 
GGCCCCATGC CCACCCGTAA CCGGCCACCC ATCAAGACCT ATGAGGCCCG GAGCCGCATT 
GGTCTCAAAC TCAAGATCAA ACAAGAGGCG GGGCTCAGCA AGGTGGTGCA CAACACTGCA 
CTGGATCCTG TGCATCAGCC CTTGCCGGCT CCAACCCCAG CGAAAGGGGC GGAGCCTCCG 
CCACACCCAG CTCCGCCCCC ACTCCCTCCT GCTACCCAGG CGCAGATGAA TGGCACTCTG 
GACCATCCCC CACCCGCAGT ACGCAAACCC ACGGTGCCTG CGTCCTGCCC ACGTCTACCA 
CTACGCAAGA CCTACCGAGA AAACATGGGC AATCCTGGTG CCGCCGAGGG TGCACAGGGA 
CGGCCGCGGG GTGCGGGCAG CCCCACCCCA CTGCCCACCA AGGTAGACGA AGCCACCAGT 
GGGCTGATCC GGGAGCTGGC AGCGGTGGAG GATGAACTAT ATCAGCGGGT TCTGAAGGGC 
GGCCCACCAC CCCCGGAGAC TCCAGCCTCC GCTACCAGCC AGGGCCCCAC TGAACCCAGT 
TGGGAAGCAC CCGTGCTACC CCCAGCCAAA CGACGCAAGT CTGAGTCCCC GGACGTGGAC 
CAGGCCAGCT TCTCTAGTGA CAGCCCGCAG GATGATACAC TTACTGAGCA TTTGCAGAGT 
GCCATCGACA GCATCCTTAA CCTGCAGCAG GCCCCCGGCC GGACACCCGC AGGCCCATAC 
CCCCATACGG GGCCCACGCC TGGCACCCCC ACATCCCCAG CGCCCCTGCA CAGGCCTGAC 
GCCTTCCCAC CCTCTAGTCA CAATGGTGGC CTCGGTGCCA GGACGTTGAA CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicra ( XM_006539918.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539981.1 Mus musculus glioma tumor suppressor candidate region gene 1 (Gltscr1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu38222
Clone ID Related Accession (Same CDS sequence)	XM_006539918.3 , XM_006539918.4
Accession Version	XM_006539918.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539918.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTCCAT GTGCAGGAAG CTGCCGCCAA CCACCTAAAC 
CCTGAGCCCA GCCAGCCTGC CCCCAGCGTG GACCTGGACT TCCTAGAAGA TGATATCTTG 
GGCTCCCCTG CAGCAGGAGG AGGTGGAGGG GGCGGCGGGG CCCCAGACCA GCCCTGTGAC 
ATCCTTCAGC AGAGTCTTCA GGAGGCCAAC ATCACAGAAC AGACCCTGGA GGCTGAGGCT 
GAACTGGACC TGGGCCCCTT CCAGCTGCCC ACCCTACAGC CCGCTGACAA TGGGGCAGGT 
GCTACTGGAG CCGCAGGAGC CACGGCAGTG ACTGCAGGAC CCCAGGCTCT CTTCCCAGGC 
AGCGCGGATC TGCTGGGGCT GCAAGCCCCG CCCACTGTAC TGACCCACCA GGCCCTGGTG 
CCACCCCAGG ATGTGGTCAA CAAGGCCTTG AGCGTCCAGC CCTTCCTGCA GCCTGTGGGC 
CTGGGCAATG TGACCCTTCA GCCCATTTCA GGCCTCCAGG GCCTTCCCAA TGGCAGTCCT 
GGGAATGCTG CAGCAGCCAC CTTGGGTCTG ACACCTATTC AAGTGGTGGG CCAGCCCGTC 
ATGGCTCTCA ACCCACCCAC CTCCCAGCTC TTGGCAAAGC AGGTACCTGT CAGTGGCTAC 
CTGGCCTCAG CAGCTGGTCC TTCAGAGCCA GTGACACTGG CATCTGCCGG CGTGTCCCCC 
CAGGGAGCCG GCCTGGTCAT CCAGAAAAAT CTTCCAGCCG CAGTGACCAC CACACTCAAC 
GGGAACTCGG TGTTTGCCGG GACAGGGGCT GCCACTGCAG CAGCCAGTGG GGCACCCTCG 
GGACAGCCGC TGGCGGTGGC CCCGGGCCTT GGCACATCAC CACTGGTACA AGCACCCAGT 
GTGATTTTAC ACAGAACCCC TACGCCTATC CAGCCCAAGC CTACAGGGGT CCTGCCCTCC 
AAACTCTACC AGCTGACACC CAAGCCCTTT CCCCCTACCG GAGCCACCCT TACCATCCAG 
GGTGAACCAG GCACCTTGCC CCAGCAGCCT AAGGCCCCCC AGAACCTGAC TTTTATGGCC 
ACGGGCAAAG CTGGCCAGAA TGTGGTGCTG TCTGGCTTCC CGGCACCGGC TTTGCAGGCG 
AATGTGTTCA AGCAGCCACC AGTCACCACC ACGGGGACAG CCCCGCCACA GCCACCAGGG 
GCCCTCAGCA AACCCATGAG CGTCCACCTC CTCAATCAAG GCAGCAGCAT CGTGATCCCA 
GCCCAGCACA TGCTGCCTGG CCAGAACCAG TTCTTGCTGC CAGGCACCCC AGCCGTACAA 
CTCCCTCAGT CACTCTCTGC ACTGCCTGCC AACGTGGGAG GCCAGATCCT CACAGCTGCA 
GCACCACACG CAGGTGGACA GCTCATTGCC AACCCTATCC TCACCAACCA GAACCTGGCA 
GGCCCACTGA GTCTGGGCCC AGTGCTGGCA CCCCACTCTG GGGCACACAG CGCTGCACAC 
ATCCTCTCTG CAGCTCCCAT CCAGGTGGGC CAGCCTGCCC TCTTCCAGAT GCCTGTGTCA 
CTGGCCACTG GCAGCCTGCC TACTCAGAGC CAGCCGGCTC CCACTGGCCC CACAGCCACC 
ACCGTCCTCC AGGGCGTCAC CCTGCCTCCC AGTGCTGTGG CCATGCTTAA CACGCCTGAT 
GGGCTAGTGC AACCCTCCAC TCCAGCTGCC ACCACTGGGG AGGCCACACC AGTTCTGGCC 
GTTCAGCCTG CAACCCAGGT GCCCCCTGCT GTCACCACAC CACTGCCTAT GGGTCTCCAA 
CAGCCACAGG CACAGCAGCC TCCACAGGTC CCTACTCCAC AGGCGGCCAC CCAGCCTCAG 
GCCACCCCTC CTCAGGCCAG CCCAAGCCTG GCTTCCAGCC CAGAGAAGAT AGTCCTGGGG 
CAGGCGCCCC CTGCGGCCAC AACGGCCATC CTCACTCAGG ATTCCCTACA GATGTTCCTG 
CCCCAGGAGA GGAGCCAGCA GCCCCTCTCT ACAGAGGGTC CCCACCTCTC GGTGCCTGCC 
TCCGTCATAG TCAGCGCCCC GCCTCCTGCC CAAGACCCAG CCCTGGCCAC GCCCGTCACC 
AAAGGAGCTG GCCTCGGCGC TCAGACCCCG GACAGCCGGG CTTCCCCAGC TCCGGCTCCC 
CAGATCCCTG CAGCTGCTCC ACTGAAAGCC CCTGGCCCCG CCTCCTCCCC CTCACTACCT 
CACCAGGCCC CCCTGGGAGA CAGTCCCCAC ATGCCCTCCC CACACCCTGC CAGGCCCCCT 
TCCCGCCCAC CCTCAAGACC CCACTCACGC CCTCCATCCC AGCCCCAGAG CCTGACCTGC 
CCACCCTCTG AGCCCACCCT GCACCCTTGC CCTCCACCCC AGGGTCCCCC AACTCTACCT 
GGCATCTTTG TCATCCAGAA TCAATTGGGC GCCCCACCAC CAGCCAGCAC CCCAGCCTCC 
ACAGCCCCGG GCCCACCCCA GCCTCCTCTG CGACCCCCAT CCCAGCCTCC AGAGGGCCCA 
CTGCCCCCAG CCTCCCACCT CCCTCCTGCC TCCACCCCCT CGGCCGTGGC CTCCTCCTCT 
GAGCCTTCTG CCAGGTTGCC GGTCCCCACA CCCCCTGACT TCCAACTCCA GTTCCCACCG 
AGCCAGGGAC CCCATAAGTC CCCTACTCCG CCACCAGCCC TCCACATGGT CCCTGAGCCC 
ACGGCACCCC CTCCTCCACC ACCTCGGACC TTCCAGATGG TAACCGCCCC CTTCCCAGCG 
TTGCCCCAGC CAAAAGCACT TCTGGAACGA TTCCACCAGG TGCCATCTGG GATTATTCTC 
CAGAATAAGG CTGGGGGTAC TCCCACCACC CCACAGACAT CCACCACCCT GGGGACCCTC 
ACCGGTCCTA CTGCCTCTGT GCTAGTCAGT GGACAGGCAC CACCTGGGAC TCCTGCCGCC 
TCTAGCCATG TCCCAGCCTC CACACCTATG GCCACCACAG GCCTCCCTCC TCTACTTCCT 
GCCGAAAACA AAGCTTTTGC CAGCAACCTT CCAACCCTGA GTGTGGCCAA AGCTACCGTG 
TCTGGGCCAG GGAAGCCCCC AGCAATTCAG TATGACAGCA AGTTGTGTAG CTTGAAGAAA 
CAGCCCCTAC TGCAACCCAG CAAAGAAGCC TGCTTCCTGG AGCATCTGCA CAAACACCAG 
GGCTCTGTCC TGCACCCCGA TTACAAGACA GCCTTCCCCT CCTTTGAGGA CGCCCTCCAT 
CGCCTCCTGC CCTACCATGT CTACCAAGGC GCCCTCCCCT CCCCCAACGA CTACCATAAA 
GTGGATGAAG AATTTGAGAC TGTCTCTACG CAGCTGCTCA AACGCACCCA GGCCATGCTC 
AATAAATATC GGCTTTTGCT TCTGGAAGAG TCCAGGAGAG TCAGTCCTTC TGCGGAGATG 
GTTATGATCG ACCGAATGTT CATTCAGGAG GAGAAGACCA CCCTTGCCTT GGATAAGCAG 
CTTGCCAAGG AGAAGCCTGA TGAGTACGTG TCTTCCTCCC GCTCCCTTGG CTTCCCTGTC 
CCAGTGTCTT CCGAGGGCCA CCGGCTCCCC AGCCATGGCC AGTCGTCTTC ATCCTCCACA 
TCTGGAACGT CTGCCCAGCC CCCTCCTCAT CTGCCCACCA AGCTAGTGAT CCGGCACGGT 
GGGGCCGGCG GCTCTCCCTC AGTGACCTGG GCCCGGGCAT CCTCCTCCTT GTCATCCACT 
TCCTCATCCT CCTCCTCATC CTCTGCTGCC TCATCCCTGG ACGCAGATGA GGACGGCCCC 
ATGCCCACCC GTAACCGGCC ACCCATCAAG ACCTATGAGG CCCGGAGCCG CATTGGTCTC 
AAACTCAAGA TCAAACAAGA GGCGGGGCTC AGCAAGGTGG TGCACAACAC TGCACTGGAT 
CCTGTGCATC AGCCCTTGCC GGCTCCAACC CCAGCGAAAG GGGCGGAGCC TCCGCCACAC 
CCAGCTCCGC CCCCACTCCC TCCTGCTACC CAGGCGCAGA TGAATGGCAC TCTGGACCAT 
CCCCCACCCG CAGTACGCAA ACCCACGGTG CCTGCGTCCT GCCCACGTCT ACCACTACGC 
AAGACCTACC GAGAAAACAT GGGCAATCCT GGTGCCGCCG AGGGTGCACA GGGACGGCCG 
CGGGGTGCGG GCAGCCCCAC CCCACTGCCC ACCAAGGTAG ACGAAGCCAC CAGTGGGCTG 
ATCCGGGAGC TGGCAGCGGT GGAGGATGAA CTATATCAGC GGGTTCTGAA GGGCGGCCCA 
CCACCCCCGG AGACTCCAGC CTCCGCTACC AGCCAGGGCC CCACTGAACC CAGTTGGGAA 
GCACCCGTGC TACCCCCAGC CAAACGACGC AAGTCTGAGT CCCCGGACGT GGACCAGGCC 
AGCTTCTCTA GTGACAGCCC GCAGGATGAT ACACTTACTG AGCATTTGCA GAGTGCCATC 
GACAGCATCC TTAACCTGCA GCAGGCCCCC GGCCGGACAC CCGCAGGCCC ATACCCCCAT 
ACGGGGCCCA CGCCTGGCAC CCCCACATCC CCAGCGCCCC TGCACAGGCC TGACGCCTTC 
CCACCCTCTA GTCACAATGG TGGCCTCGGT GCCAGGACGT TGAACAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539981.1
CDS	297..4967
Translation	MDDEDGRCLLDVICDPQALNDFLHGSEKLHVQEAAANHLNPEPSQPAPSVDLDFLEDDILGSPAAGGGGGGGGAPDQPCDILQQSLQEANITEQTLEAEAELDLGPFQLPTLQPADNGAGATGAAGATAVTAGPQALFPGSADLLGLQAPPTVLTHQALVPPQDVVNKALSVQPFLQPVGLGNVTLQPISGLQGLPNGSPGNAAAATLGLTPIQVVGQPVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLPAAVTTTLNGNSVFAGTGAATAAASGAPSGQPLAVAPGLGTSPLVQAPSVILHRTPTPIQPKPTGVLPSKLYQLTPKPFPPTGATLTIQGEPGTLPQQPKAPQNLTFMATGKAGQNVVLSGFPAPALQANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTPAVQLPQSLSALPANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAAHILSAAPIQVGQPALFQMPVSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNTPDGLVQPSTPAATTGEATPVLAVQPATQVPPAVTTPLPMGLQQPQAQQPPQVPTPQAATQPQATPPQASPSLASSPEKIVLGQAPPAATTAILTQDSLQMFLPQERSQQPLSTEGPHLSVPASVIVSAPPPAQDPALATPVTKGAGLGAQTPDSRASPAPAPQIPAAAPLKAPGPASSPSLPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPTLPGIFVIQNQLGAPPPASTPASTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVASSSEPSARLPVPTPPDFQLQFPPSQGPHKSPTPPPALHMVPEPTAPPPPPPRTFQMVTAPFPALPQPKALLERFHQVPSGIILQNKAGGTPTTPQTSTTLGTLTGPTASVLVSGQAPPGTPAASSHVPASTPMATTGLPPLLPAENKAFASNLPTLSVAKATVSGPGKPPAIQYDSKLCSLKKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPNDYHKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALDKQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGQSSSSSTSGTSAQPPPHLPTKLVIRHGGAGGSPSVTWARASSSLSSTSSSSSSSSAASSLDADEDGPMPTRNRPPIKTYEARSRIGLKLKIKQEAGLSKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTLDHPPPAVRKPTVPASCPRLPLRKTYRENMGNPGAAEGAQGRPRGAGSPTPLPTKVDEATSGLIRELAAVEDELYQRVLKGGPPPPETPASATSQGPTEPSWEAPVLPPAKRRKSESPDVDQASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPHTGPTPGTPTSPAPLHRPDAFPPSSHNGGLGARTLNR

Target ORF information:

RefSeq Version	XM_006539918.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus glioma tumor suppressor candidate region gene 1 (Gltscr1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539918.3

ORF Insert Sequence:

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTCCAT GTGCAGGAAG CTGCCGCCAA CCACCTAAAC 
CCTGAGCCCA GCCAGCCTGC CCCCAGCGTG GACCTGGACT TCCTAGAAGA TGATATCTTG 
GGCTCCCCTG CAGCAGGAGG AGGTGGAGGG GGCGGCGGGG CCCCAGACCA GCCCTGTGAC 
ATCCTTCAGC AGAGTCTTCA GGAGGCCAAC ATCACAGAAC AGACCCTGGA GGCTGAGGCT 
GAACTGGACC TGGGCCCCTT CCAGCTGCCC ACCCTACAGC CCGCTGACAA TGGGGCAGGT 
GCTACTGGAG CCGCAGGAGC CACGGCAGTG ACTGCAGGAC CCCAGGCTCT CTTCCCAGGC 
AGCGCGGATC TGCTGGGGCT GCAAGCCCCG CCCACTGTAC TGACCCACCA GGCCCTGGTG 
CCACCCCAGG ATGTGGTCAA CAAGGCCTTG AGCGTCCAGC CCTTCCTGCA GCCTGTGGGC 
CTGGGCAATG TGACCCTTCA GCCCATTTCA GGCCTCCAGG GCCTTCCCAA TGGCAGTCCT 
GGGAATGCTG CAGCAGCCAC CTTGGGTCTG ACACCTATTC AAGTGGTGGG CCAGCCCGTC 
ATGGCTCTCA ACCCACCCAC CTCCCAGCTC TTGGCAAAGC AGGTACCTGT CAGTGGCTAC 
CTGGCCTCAG CAGCTGGTCC TTCAGAGCCA GTGACACTGG CATCTGCCGG CGTGTCCCCC 
CAGGGAGCCG GCCTGGTCAT CCAGAAAAAT CTTCCAGCCG CAGTGACCAC CACACTCAAC 
GGGAACTCGG TGTTTGCCGG GACAGGGGCT GCCACTGCAG CAGCCAGTGG GGCACCCTCG 
GGACAGCCGC TGGCGGTGGC CCCGGGCCTT GGCACATCAC CACTGGTACA AGCACCCAGT 
GTGATTTTAC ACAGAACCCC TACGCCTATC CAGCCCAAGC CTACAGGGGT CCTGCCCTCC 
AAACTCTACC AGCTGACACC CAAGCCCTTT CCCCCTACCG GAGCCACCCT TACCATCCAG 
GGTGAACCAG GCACCTTGCC CCAGCAGCCT AAGGCCCCCC AGAACCTGAC TTTTATGGCC 
ACGGGCAAAG CTGGCCAGAA TGTGGTGCTG TCTGGCTTCC CGGCACCGGC TTTGCAGGCG 
AATGTGTTCA AGCAGCCACC AGTCACCACC ACGGGGACAG CCCCGCCACA GCCACCAGGG 
GCCCTCAGCA AACCCATGAG CGTCCACCTC CTCAATCAAG GCAGCAGCAT CGTGATCCCA 
GCCCAGCACA TGCTGCCTGG CCAGAACCAG TTCTTGCTGC CAGGCACCCC AGCCGTACAA 
CTCCCTCAGT CACTCTCTGC ACTGCCTGCC AACGTGGGAG GCCAGATCCT CACAGCTGCA 
GCACCACACG CAGGTGGACA GCTCATTGCC AACCCTATCC TCACCAACCA GAACCTGGCA 
GGCCCACTGA GTCTGGGCCC AGTGCTGGCA CCCCACTCTG GGGCACACAG CGCTGCACAC 
ATCCTCTCTG CAGCTCCCAT CCAGGTGGGC CAGCCTGCCC TCTTCCAGAT GCCTGTGTCA 
CTGGCCACTG GCAGCCTGCC TACTCAGAGC CAGCCGGCTC CCACTGGCCC CACAGCCACC 
ACCGTCCTCC AGGGCGTCAC CCTGCCTCCC AGTGCTGTGG CCATGCTTAA CACGCCTGAT 
GGGCTAGTGC AACCCTCCAC TCCAGCTGCC ACCACTGGGG AGGCCACACC AGTTCTGGCC 
GTTCAGCCTG CAACCCAGGT GCCCCCTGCT GTCACCACAC CACTGCCTAT GGGTCTCCAA 
CAGCCACAGG CACAGCAGCC TCCACAGGTC CCTACTCCAC AGGCGGCCAC CCAGCCTCAG 
GCCACCCCTC CTCAGGCCAG CCCAAGCCTG GCTTCCAGCC CAGAGAAGAT AGTCCTGGGG 
CAGGCGCCCC CTGCGGCCAC AACGGCCATC CTCACTCAGG ATTCCCTACA GATGTTCCTG 
CCCCAGGAGA GGAGCCAGCA GCCCCTCTCT ACAGAGGGTC CCCACCTCTC GGTGCCTGCC 
TCCGTCATAG TCAGCGCCCC GCCTCCTGCC CAAGACCCAG CCCTGGCCAC GCCCGTCACC 
AAAGGAGCTG GCCTCGGCGC TCAGACCCCG GACAGCCGGG CTTCCCCAGC TCCGGCTCCC 
CAGATCCCTG CAGCTGCTCC ACTGAAAGCC CCTGGCCCCG CCTCCTCCCC CTCACTACCT 
CACCAGGCCC CCCTGGGAGA CAGTCCCCAC ATGCCCTCCC CACACCCTGC CAGGCCCCCT 
TCCCGCCCAC CCTCAAGACC CCACTCACGC CCTCCATCCC AGCCCCAGAG CCTGACCTGC 
CCACCCTCTG AGCCCACCCT GCACCCTTGC CCTCCACCCC AGGGTCCCCC AACTCTACCT 
GGCATCTTTG TCATCCAGAA TCAATTGGGC GCCCCACCAC CAGCCAGCAC CCCAGCCTCC 
ACAGCCCCGG GCCCACCCCA GCCTCCTCTG CGACCCCCAT CCCAGCCTCC AGAGGGCCCA 
CTGCCCCCAG CCTCCCACCT CCCTCCTGCC TCCACCCCCT CGGCCGTGGC CTCCTCCTCT 
GAGCCTTCTG CCAGGTTGCC GGTCCCCACA CCCCCTGACT TCCAACTCCA GTTCCCACCG 
AGCCAGGGAC CCCATAAGTC CCCTACTCCG CCACCAGCCC TCCACATGGT CCCTGAGCCC 
ACGGCACCCC CTCCTCCACC ACCTCGGACC TTCCAGATGG TAACCGCCCC CTTCCCAGCG 
TTGCCCCAGC CAAAAGCACT TCTGGAACGA TTCCACCAGG TGCCATCTGG GATTATTCTC 
CAGAATAAGG CTGGGGGTAC TCCCACCACC CCACAGACAT CCACCACCCT GGGGACCCTC 
ACCGGTCCTA CTGCCTCTGT GCTAGTCAGT GGACAGGCAC CACCTGGGAC TCCTGCCGCC 
TCTAGCCATG TCCCAGCCTC CACACCTATG GCCACCACAG GCCTCCCTCC TCTACTTCCT 
GCCGAAAACA AAGCTTTTGC CAGCAACCTT CCAACCCTGA GTGTGGCCAA AGCTACCGTG 
TCTGGGCCAG GGAAGCCCCC AGCAATTCAG TATGACAGCA AGTTGTGTAG CTTGAAGAAA 
CAGCCCCTAC TGCAACCCAG CAAAGAAGCC TGCTTCCTGG AGCATCTGCA CAAACACCAG 
GGCTCTGTCC TGCACCCCGA TTACAAGACA GCCTTCCCCT CCTTTGAGGA CGCCCTCCAT 
CGCCTCCTGC CCTACCATGT CTACCAAGGC GCCCTCCCCT CCCCCAACGA CTACCATAAA 
GTGGATGAAG AATTTGAGAC TGTCTCTACG CAGCTGCTCA AACGCACCCA GGCCATGCTC 
AATAAATATC GGCTTTTGCT TCTGGAAGAG TCCAGGAGAG TCAGTCCTTC TGCGGAGATG 
GTTATGATCG ACCGAATGTT CATTCAGGAG GAGAAGACCA CCCTTGCCTT GGATAAGCAG 
CTTGCCAAGG AGAAGCCTGA TGAGTACGTG TCTTCCTCCC GCTCCCTTGG CTTCCCTGTC 
CCAGTGTCTT CCGAGGGCCA CCGGCTCCCC AGCCATGGCC AGTCGTCTTC ATCCTCCACA 
TCTGGAACGT CTGCCCAGCC CCCTCCTCAT CTGCCCACCA AGCTAGTGAT CCGGCACGGT 
GGGGCCGGCG GCTCTCCCTC AGTGACCTGG GCCCGGGCAT CCTCCTCCTT GTCATCCACT 
TCCTCATCCT CCTCCTCATC CTCTGCTGCC TCATCCCTGG ACGCAGATGA GGACGGCCCC 
ATGCCCACCC GTAACCGGCC ACCCATCAAG ACCTATGAGG CCCGGAGCCG CATTGGTCTC 
AAACTCAAGA TCAAACAAGA GGCGGGGCTC AGCAAGGTGG TGCACAACAC TGCACTGGAT 
CCTGTGCATC AGCCCTTGCC GGCTCCAACC CCAGCGAAAG GGGCGGAGCC TCCGCCACAC 
CCAGCTCCGC CCCCACTCCC TCCTGCTACC CAGGCGCAGA TGAATGGCAC TCTGGACCAT 
CCCCCACCCG CAGTACGCAA ACCCACGGTG CCTGCGTCCT GCCCACGTCT ACCACTACGC 
AAGACCTACC GAGAAAACAT GGGCAATCCT GGTGCCGCCG AGGGTGCACA GGGACGGCCG 
CGGGGTGCGG GCAGCCCCAC CCCACTGCCC ACCAAGGTAG ACGAAGCCAC CAGTGGGCTG 
ATCCGGGAGC TGGCAGCGGT GGAGGATGAA CTATATCAGC GGGTTCTGAA GGGCGGCCCA 
CCACCCCCGG AGACTCCAGC CTCCGCTACC AGCCAGGGCC CCACTGAACC CAGTTGGGAA 
GCACCCGTGC TACCCCCAGC CAAACGACGC AAGTCTGAGT CCCCGGACGT GGACCAGGCC 
AGCTTCTCTA GTGACAGCCC GCAGGATGAT ACACTTACTG AGCATTTGCA GAGTGCCATC 
GACAGCATCC TTAACCTGCA GCAGGCCCCC GGCCGGACAC CCGCAGGCCC ATACCCCCAT 
ACGGGGCCCA CGCCTGGCAC CCCCACATCC CCAGCGCCCC TGCACAGGCC TGACGCCTTC 
CCACCCTCTA GTCACAATGG TGGCCTCGGT GCCAGGACGT TGAACAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bicra ( XM_011250559.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248861.1 Mus musculus glioma tumor suppressor candidate region gene 1 (Gltscr1), transcript variant X3, mRNA.

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CloneID	OMu74158
Clone ID Related Accession (Same CDS sequence)	XM_011250559.2 , XM_011250559.3
Accession Version	XM_011250559.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	glioma tumor suppressor candidate region gene 1 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGATCCCTGC AGCTGCTCCA CTGAAAGCCC CTGGCCCCGC CTCCTCCCCC 
TCACTACCTC ACCAGGCCCC CCTGGGAGAC AGTCCCCACA TGCCCTCCCC ACACCCTGCC 
AGGCCCCCTT CCCGCCCACC CTCAAGACCC CACTCACGCC CTCCATCCCA GCCCCAGAGC 
CTGACCTGCC CACCCTCTGA GCCCACCCTG CACCCTTGCC CTCCACCCCA GGGTCCCCCA 
ACTCTACCTG GCATCTTTGT CATCCAGAAT CAATTGGGCG CCCCACCACC AGCCAGCACC 
CCAGCCTCCA CAGCCCCGGG CCCACCCCAG CCTCCTCTGC GACCCCCATC CCAGCCTCCA 
GAGGGCCCAC TGCCCCCAGC CTCCCACCTC CCTCCTGCCT CCACCCCCTC GGCCGTGGCC 
TCCTCCTCTG AGCCTTCTGC CAGGTTGCCG GTCCCCACAC CCCCTGACTT CCAACTCCAG 
TTCCCACCGA GCCAGGGACC CCATAAGTCC CCTACTCCGC CACCAGCCCT CCACATGGTC 
CCTGAGCCCA CGGCACCCCC TCCTCCACCA CCTCGGACCT TCCAGATGGT AACCGCCCCC 
TTCCCAGCGT TGCCCCAGCC AAAAGCACTT CTGGAACGAT TCCACCAGGT GCCATCTGGG 
ATTATTCTCC AGAATAAGGC TGGGGGTACT CCCACCACCC CACAGACATC CACCACCCTG 
GGGACCCTCA CCGGTCCTAC TGCCTCTGTG CTAGTCAGTG GACAGGCACC ACCTGGGACT 
CCTGCCGCCT CTAGCCATGT CCCAGCCTCC ACACCTATGG CCACCACAGG CCTCCCTCCT 
CTACTTCCTG CCGAAAACAA AGCTTTTGCC AGCAACCTTC CAACCCTGAG TGTGGCCAAA 
GCTACCGTGT CTGGGCCAGG GAAGCCCCCA GCAATTCAGT ATGACAGCAA GTTGTGTAGC 
TTGAAGAAAC AGCCCCTACT GCAACCCAGC AAAGAAGCCT GCTTCCTGGA GCATCTGCAC 
AAACACCAGG GCTCTGTCCT GCACCCCGAT TACAAGACAG CCTTCCCCTC CTTTGAGGAC 
GCCCTCCATC GCCTCCTGCC CTACCATGTC TACCAAGGCG CCCTCCCCTC CCCCAACGAC 
TACCATAAAG TGGATGAAGA ATTTGAGACT GTCTCTACGC AGCTGCTCAA ACGCACCCAG 
GCCATGCTCA ATAAATATCG GCTTTTGCTT CTGGAAGAGT CCAGGAGAGT CAGTCCTTCT 
GCGGAGATGG TTATGATCGA CCGAATGTTC ATTCAGGAGG AGAAGACCAC CCTTGCCTTG 
GATAAGCAGC TTGCCAAGGA GAAGCCTGAT GAGTACGTGT CTTCCTCCCG CTCCCTTGGC 
TTCCCTGTCC CAGTGTCTTC CGAGGGCCAC CGGCTCCCCA GCCATGGCCA GTCGTCTTCA 
TCCTCCACAT CTGGAACGTC TGCCCAGCCC CCTCCTCATC TGCCCACCAA GCTAGTGATC 
CGGCACGGTG GGGCCGGCGG CTCTCCCTCA GTGACCTGGG CCCGGGCATC CTCCTCCTTG 
TCATCCACTT CCTCATCCTC CTCCTCATCC TCTGCTGCCT CATCCCTGGA CGCAGATGAG 
GACGGCCCCA TGCCCACCCG TAACCGGCCA CCCATCAAGA CCTATGAGGC CCGGAGCCGC 
ATTGGTCTCA AACTCAAGAT CAAACAAGAG GCGGGGCTCA GCAAGGTGGT GCACAACACT 
GCACTGGATC CTGTGCATCA GCCCTTGCCG GCTCCAACCC CAGCGAAAGG GGCGGAGCCT 
CCGCCACACC CAGCTCCGCC CCCACTCCCT CCTGCTACCC AGGCGCAGAT GAATGGCACT 
CTGGACCATC CCCCACCCGC AGTACGCAAA CCCACGGTGC CTGCGTCCTG CCCACGTCTA 
CCACTACGCA AGACCTACCG AGAAAACATG GGCAATCCTG GTGCCGCCGA GGGTGCACAG 
GGACGGCCGC GGGGTGCGGG CAGCCCCACC CCACTGCCCA CCAAGGTAGA CGAAGCCACC 
AGTGGGCTGA TCCGGGAGCT GGCAGCGGTG GAGGATGAAC TATATCAGCG GGTTCTGAAG 
GGCGGCCCAC CACCCCCGGA GACTCCAGCC TCCGCTACCA GCCAGGGCCC CACTGAACCC 
AGTTGGGAAG CACCCGTGCT ACCCCCAGCC AAACGACGCA AGTCTGAGTC CCCGGACGTG 
GACCAGGCCA GCTTCTCTAG TGACAGCCCG CAGGATGATA CACTTACTGA GCATTTGCAG 
AGTGCCATCG ACAGCATCCT TAACCTGCAG CAGGCCCCCG GCCGGACACC CGCAGGCCCA 
TACCCCCATA CGGGGCCCAC GCCTGGCACC CCCACATCCC CAGCGCCCCT GCACAGGCCT 
GACGCCTTCC CACCCTCTAG TCACAATGGT GGCCTCGGTG CCAGGACGTT GAACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248861.1
CDS	297..4856
Translation	MDDEDGRCLLDVICDPQALNDFLHGSEKLDSDDLLDAPVEAQSAFYEGPGLHVQEAAANHLNPEPSQPAPSVDLDFLEDDILGSPAAGGGGGGGGAPDQPCDILQQSLQEANITEQTLEAEAELDLGPFQLPTLQPADNGAGATGAAGATAVTAGPQALFPGSADLLGLQAPPTVLTHQALVPPQDVVNKALSVQPFLQPVGLGNVTLQPISGLQGLPNGSPGNAAAATLGLTPIQVVGQPVMALNPPTSQLLAKQVPVSGYLASAAGPSEPVTLASAGVSPQGAGLVIQKNLPAAVTTTLNGNSVFAGTGAATAAASGAPSGQPLAVAPGLGTSPLVQAPSVILHRTPTPIQPKPTGVLPSKLYQLTPKPFPPTGATLTIQGEPGTLPQQPKAPQNLTFMATGKAGQNVVLSGFPAPALQANVFKQPPVTTTGTAPPQPPGALSKPMSVHLLNQGSSIVIPAQHMLPGQNQFLLPGTPAVQLPQSLSALPANVGGQILTAAAPHAGGQLIANPILTNQNLAGPLSLGPVLAPHSGAHSAAHILSAAPIQVGQPALFQMPVSLATGSLPTQSQPAPTGPTATTVLQGVTLPPSAVAMLNTPDGLVQPSTPAATTGEATPVLAVQPATQVPPAVTTPLPMGLQQPQAQQPPQVPTPQAATQPQATPPQASPSLASSPEKIVLGQAPPAATTAILTQDSLQMFLPQIPAAAPLKAPGPASSPSLPHQAPLGDSPHMPSPHPARPPSRPPSRPHSRPPSQPQSLTCPPSEPTLHPCPPPQGPPTLPGIFVIQNQLGAPPPASTPASTAPGPPQPPLRPPSQPPEGPLPPASHLPPASTPSAVASSSEPSARLPVPTPPDFQLQFPPSQGPHKSPTPPPALHMVPEPTAPPPPPPRTFQMVTAPFPALPQPKALLERFHQVPSGIILQNKAGGTPTTPQTSTTLGTLTGPTASVLVSGQAPPGTPAASSHVPASTPMATTGLPPLLPAENKAFASNLPTLSVAKATVSGPGKPPAIQYDSKLCSLKKQPLLQPSKEACFLEHLHKHQGSVLHPDYKTAFPSFEDALHRLLPYHVYQGALPSPNDYHKVDEEFETVSTQLLKRTQAMLNKYRLLLLEESRRVSPSAEMVMIDRMFIQEEKTTLALDKQLAKEKPDEYVSSSRSLGFPVPVSSEGHRLPSHGQSSSSSTSGTSAQPPPHLPTKLVIRHGGAGGSPSVTWARASSSLSSTSSSSSSSSAASSLDADEDGPMPTRNRPPIKTYEARSRIGLKLKIKQEAGLSKVVHNTALDPVHQPLPAPTPAKGAEPPPHPAPPPLPPATQAQMNGTLDHPPPAVRKPTVPASCPRLPLRKTYRENMGNPGAAEGAQGRPRGAGSPTPLPTKVDEATSGLIRELAAVEDELYQRVLKGGPPPPETPASATSQGPTEPSWEAPVLPPAKRRKSESPDVDQASFSSDSPQDDTLTEHLQSAIDSILNLQQAPGRTPAGPYPHTGPTPGTPTSPAPLHRPDAFPPSSHNGGLGARTLNR

Target ORF information:

RefSeq Version	XM_011250559.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus glioma tumor suppressor candidate region gene 1 (Gltscr1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250559.2

ORF Insert Sequence:

ATGGATGATG AGGATGGGAG ATGCTTACTA GACGTGATTT GTGATCCTCA GGCCCTCAAT 
GATTTCTTGC ATGGATCCGA GAAGCTGGAC AGCGATGACC TCCTGGATGC CCCTGTGGAG 
GCCCAAAGTG CCTTCTATGA AGGTCCTGGG CTCCATGTGC AGGAAGCTGC CGCCAACCAC 
CTAAACCCTG AGCCCAGCCA GCCTGCCCCC AGCGTGGACC TGGACTTCCT AGAAGATGAT 
ATCTTGGGCT CCCCTGCAGC AGGAGGAGGT GGAGGGGGCG GCGGGGCCCC AGACCAGCCC 
TGTGACATCC TTCAGCAGAG TCTTCAGGAG GCCAACATCA CAGAACAGAC CCTGGAGGCT 
GAGGCTGAAC TGGACCTGGG CCCCTTCCAG CTGCCCACCC TACAGCCCGC TGACAATGGG 
GCAGGTGCTA CTGGAGCCGC AGGAGCCACG GCAGTGACTG CAGGACCCCA GGCTCTCTTC 
CCAGGCAGCG CGGATCTGCT GGGGCTGCAA GCCCCGCCCA CTGTACTGAC CCACCAGGCC 
CTGGTGCCAC CCCAGGATGT GGTCAACAAG GCCTTGAGCG TCCAGCCCTT CCTGCAGCCT 
GTGGGCCTGG GCAATGTGAC CCTTCAGCCC ATTTCAGGCC TCCAGGGCCT TCCCAATGGC 
AGTCCTGGGA ATGCTGCAGC AGCCACCTTG GGTCTGACAC CTATTCAAGT GGTGGGCCAG 
CCCGTCATGG CTCTCAACCC ACCCACCTCC CAGCTCTTGG CAAAGCAGGT ACCTGTCAGT 
GGCTACCTGG CCTCAGCAGC TGGTCCTTCA GAGCCAGTGA CACTGGCATC TGCCGGCGTG 
TCCCCCCAGG GAGCCGGCCT GGTCATCCAG AAAAATCTTC CAGCCGCAGT GACCACCACA 
CTCAACGGGA ACTCGGTGTT TGCCGGGACA GGGGCTGCCA CTGCAGCAGC CAGTGGGGCA 
CCCTCGGGAC AGCCGCTGGC GGTGGCCCCG GGCCTTGGCA CATCACCACT GGTACAAGCA 
CCCAGTGTGA TTTTACACAG AACCCCTACG CCTATCCAGC CCAAGCCTAC AGGGGTCCTG 
CCCTCCAAAC TCTACCAGCT GACACCCAAG CCCTTTCCCC CTACCGGAGC CACCCTTACC 
ATCCAGGGTG AACCAGGCAC CTTGCCCCAG CAGCCTAAGG CCCCCCAGAA CCTGACTTTT 
ATGGCCACGG GCAAAGCTGG CCAGAATGTG GTGCTGTCTG GCTTCCCGGC ACCGGCTTTG 
CAGGCGAATG TGTTCAAGCA GCCACCAGTC ACCACCACGG GGACAGCCCC GCCACAGCCA 
CCAGGGGCCC TCAGCAAACC CATGAGCGTC CACCTCCTCA ATCAAGGCAG CAGCATCGTG 
ATCCCAGCCC AGCACATGCT GCCTGGCCAG AACCAGTTCT TGCTGCCAGG CACCCCAGCC 
GTACAACTCC CTCAGTCACT CTCTGCACTG CCTGCCAACG TGGGAGGCCA GATCCTCACA 
GCTGCAGCAC CACACGCAGG TGGACAGCTC ATTGCCAACC CTATCCTCAC CAACCAGAAC 
CTGGCAGGCC CACTGAGTCT GGGCCCAGTG CTGGCACCCC ACTCTGGGGC ACACAGCGCT 
GCACACATCC TCTCTGCAGC TCCCATCCAG GTGGGCCAGC CTGCCCTCTT CCAGATGCCT 
GTGTCACTGG CCACTGGCAG CCTGCCTACT CAGAGCCAGC CGGCTCCCAC TGGCCCCACA 
GCCACCACCG TCCTCCAGGG CGTCACCCTG CCTCCCAGTG CTGTGGCCAT GCTTAACACG 
CCTGATGGGC TAGTGCAACC CTCCACTCCA GCTGCCACCA CTGGGGAGGC CACACCAGTT 
CTGGCCGTTC AGCCTGCAAC CCAGGTGCCC CCTGCTGTCA CCACACCACT GCCTATGGGT 
CTCCAACAGC CACAGGCACA GCAGCCTCCA CAGGTCCCTA CTCCACAGGC GGCCACCCAG 
CCTCAGGCCA CCCCTCCTCA GGCCAGCCCA AGCCTGGCTT CCAGCCCAGA GAAGATAGTC 
CTGGGGCAGG CGCCCCCTGC GGCCACAACG GCCATCCTCA CTCAGGATTC CCTACAGATG 
TTCCTGCCCC AGATCCCTGC AGCTGCTCCA CTGAAAGCCC CTGGCCCCGC CTCCTCCCCC 
TCACTACCTC ACCAGGCCCC CCTGGGAGAC AGTCCCCACA TGCCCTCCCC ACACCCTGCC 
AGGCCCCCTT CCCGCCCACC CTCAAGACCC CACTCACGCC CTCCATCCCA GCCCCAGAGC 
CTGACCTGCC CACCCTCTGA GCCCACCCTG CACCCTTGCC CTCCACCCCA GGGTCCCCCA 
ACTCTACCTG GCATCTTTGT CATCCAGAAT CAATTGGGCG CCCCACCACC AGCCAGCACC 
CCAGCCTCCA CAGCCCCGGG CCCACCCCAG CCTCCTCTGC GACCCCCATC CCAGCCTCCA 
GAGGGCCCAC TGCCCCCAGC CTCCCACCTC CCTCCTGCCT CCACCCCCTC GGCCGTGGCC 
TCCTCCTCTG AGCCTTCTGC CAGGTTGCCG GTCCCCACAC CCCCTGACTT CCAACTCCAG 
TTCCCACCGA GCCAGGGACC CCATAAGTCC CCTACTCCGC CACCAGCCCT CCACATGGTC 
CCTGAGCCCA CGGCACCCCC TCCTCCACCA CCTCGGACCT TCCAGATGGT AACCGCCCCC 
TTCCCAGCGT TGCCCCAGCC AAAAGCACTT CTGGAACGAT TCCACCAGGT GCCATCTGGG 
ATTATTCTCC AGAATAAGGC TGGGGGTACT CCCACCACCC CACAGACATC CACCACCCTG 
GGGACCCTCA CCGGTCCTAC TGCCTCTGTG CTAGTCAGTG GACAGGCACC ACCTGGGACT 
CCTGCCGCCT CTAGCCATGT CCCAGCCTCC ACACCTATGG CCACCACAGG CCTCCCTCCT 
CTACTTCCTG CCGAAAACAA AGCTTTTGCC AGCAACCTTC CAACCCTGAG TGTGGCCAAA 
GCTACCGTGT CTGGGCCAGG GAAGCCCCCA GCAATTCAGT ATGACAGCAA GTTGTGTAGC 
TTGAAGAAAC AGCCCCTACT GCAACCCAGC AAAGAAGCCT GCTTCCTGGA GCATCTGCAC 
AAACACCAGG GCTCTGTCCT GCACCCCGAT TACAAGACAG CCTTCCCCTC CTTTGAGGAC 
GCCCTCCATC GCCTCCTGCC CTACCATGTC TACCAAGGCG CCCTCCCCTC CCCCAACGAC 
TACCATAAAG TGGATGAAGA ATTTGAGACT GTCTCTACGC AGCTGCTCAA ACGCACCCAG 
GCCATGCTCA ATAAATATCG GCTTTTGCTT CTGGAAGAGT CCAGGAGAGT CAGTCCTTCT 
GCGGAGATGG TTATGATCGA CCGAATGTTC ATTCAGGAGG AGAAGACCAC CCTTGCCTTG 
GATAAGCAGC TTGCCAAGGA GAAGCCTGAT GAGTACGTGT CTTCCTCCCG CTCCCTTGGC 
TTCCCTGTCC CAGTGTCTTC CGAGGGCCAC CGGCTCCCCA GCCATGGCCA GTCGTCTTCA 
TCCTCCACAT CTGGAACGTC TGCCCAGCCC CCTCCTCATC TGCCCACCAA GCTAGTGATC 
CGGCACGGTG GGGCCGGCGG CTCTCCCTCA GTGACCTGGG CCCGGGCATC CTCCTCCTTG 
TCATCCACTT CCTCATCCTC CTCCTCATCC TCTGCTGCCT CATCCCTGGA CGCAGATGAG 
GACGGCCCCA TGCCCACCCG TAACCGGCCA CCCATCAAGA CCTATGAGGC CCGGAGCCGC 
ATTGGTCTCA AACTCAAGAT CAAACAAGAG GCGGGGCTCA GCAAGGTGGT GCACAACACT 
GCACTGGATC CTGTGCATCA GCCCTTGCCG GCTCCAACCC CAGCGAAAGG GGCGGAGCCT 
CCGCCACACC CAGCTCCGCC CCCACTCCCT CCTGCTACCC AGGCGCAGAT GAATGGCACT 
CTGGACCATC CCCCACCCGC AGTACGCAAA CCCACGGTGC CTGCGTCCTG CCCACGTCTA 
CCACTACGCA AGACCTACCG AGAAAACATG GGCAATCCTG GTGCCGCCGA GGGTGCACAG 
GGACGGCCGC GGGGTGCGGG CAGCCCCACC CCACTGCCCA CCAAGGTAGA CGAAGCCACC 
AGTGGGCTGA TCCGGGAGCT GGCAGCGGTG GAGGATGAAC TATATCAGCG GGTTCTGAAG 
GGCGGCCCAC CACCCCCGGA GACTCCAGCC TCCGCTACCA GCCAGGGCCC CACTGAACCC 
AGTTGGGAAG CACCCGTGCT ACCCCCAGCC AAACGACGCA AGTCTGAGTC CCCGGACGTG 
GACCAGGCCA GCTTCTCTAG TGACAGCCCG CAGGATGATA CACTTACTGA GCATTTGCAG 
AGTGCCATCG ACAGCATCCT TAACCTGCAG CAGGCCCCCG GCCGGACACC CGCAGGCCCA 
TACCCCCATA CGGGGCCCAC GCCTGGCACC CCCACATCCC CAGCGCCCCT GCACAGGCCT 
GACGCCTTCC CACCCTCTAG TCACAATGGT GGCCTCGGTG CCAGGACGTT GAACAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The gene for soluble N-ethylmaleimide sensitive factor attachment protein alpha is mutated in hydrocephaly with hop gait (hyh) mice.
Proceedings of the National Academy of Sciences of the United States of America101(6)1748-53(2004 Feb)
Hong HK,Chakravarti A,Takahashi JS

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Mapping of the mouse hyh gene to a YAC/BAC contig on proximal Chromosome 7.
Mammalian genome : official journal of the International Mammalian Genome Society13(5)239-44(2002 May)
Chae TH,Allen KM,Davisson MT,Sweet HO,Walsh CA

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Bicra cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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