Macc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Macc1
Entrez Gene ID	238455
Full Name	metastasis associated in colon cancer 1
Synonyms	4732474O15Rik,Gm267
General protein information	Preferred Names metastasis associated in colon cancer 1 Names metastasis-associated in colon cancer protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 F2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515861.2	XP_006515924.1	metastasis-associated in colon cancer protein 1 isoform X1
XM_006515861.3	XP_006515924.1	metastasis-associated in colon cancer protein 1 isoform X1
XM_006515863.3	XP_006515926.1	metastasis-associated in colon cancer protein 1 isoform X2
NM_001163136.1	NP_001156608.1	metastasis-associated in colon cancer protein 1
XM_006515862.2	XP_006515925.1	metastasis-associated in colon cancer protein 1 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MACC1	XP_418705.4
Canis lupus familiaris (dog)	MACC1	XP_005628662.1
Bos taurus (cattle)	MACC1	XP_002686732.1
Mus musculus (house mouse)	Macc1	NP_001156608.1
Danio rerio (zebrafish)	macc1	NP_001186695.1
Rattus norvegicus (Norway rat)	Macc1	NP_001178704.1
Xenopus tropicalis (tropical clawed frog)	macc1	XP_002933345.1
Homo sapiens (human)	MACC1	NP_877439.3
Pan troglodytes (chimpanzee)	MACC1	XP_003318388.1

Gene Ontology
Bibliography

Related articles in PubMed

Metastasis-associated in colon cancer-1 upregulates vascular endothelial growth factor-C/D to promote lymphangiogenesis in human gastric cancer.
Sun L, Duan J, Jiang Y, Wang L, Huang N, Lin L, Liao Y, Liao W
Cancer letters357(1)242-53(2015 Feb)

Analysis of HGF, MACC1, C-met and apoptosis-related genes in cervical carcinoma mice.
Chen XP, Ren XP, Lan JY, Chen YG, Shen ZJ
Molecular biology reports41(3)1247-56(2014 Mar)

In vivo imaging of colorectal cancer growth and metastasis by targeting MACC1 with shRNA in xenografted mice.
Pichorner A, Sack U, Kobelt D, Kelch I, Arlt F, Smith J, Walther W, Schlag PM, Stein U
Clinical & experimental metastasis29(6)573-83(2012 Aug)

MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis.
Stein U, Walther W, Arlt F, Schwabe H, Smith J, Fichtner I, Birchmeier W, Schlag PM
Nature medicine15(1)59-67(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest a critical role for MACC1 in lymphatic dissemination of GC, providing evidence that MACC1 upregulated VEGF-C/VEGF-D secretion to promote lymphangiogenesis via c-Met signaling.
Title: Metastasis-associated in colon cancer-1 upregulates vascular endothelial growth factor-C/D to promote lymphangiogenesis in human gastric cancer.

HGF, MACC1 and C-met genes involve into malignant cervical tumors occurrence, development and prognosis, and might become potential molecular target therapy site of cervical cancer
Title: Analysis of HGF, MACC1, C-met and apoptosis-related genes in cervical carcinoma mice.

MACC1 has a role in colorectal cancer and can be used for imaging in xenografts
Title: In vivo imaging of colorectal cancer growth and metastasis by targeting MACC1 with shRNA in xenografted mice.

MACC1 will be useful for the identification of poor prognosis subjects with colorectal cancer and is a promising new target for intervention in metastasis formation.
Title: MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis.

The following Macc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Macc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08749	NM_001163136.1 Latest version!	Mus musculus metastasis associated in colon cancer 1 (Macc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu08749	XM_006515863.3 Latest version!	Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu08749	XM_006515862.2 Latest version!	Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu47837	XM_006515861.3 Latest version!	Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu47837	XM_006515861.2	Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Macc1 ( NM_001163136.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001156608.1 Mus musculus metastasis associated in colon cancer 1 (Macc1), mRNA.

Price & Availability↑

CloneID	OMu08749
Clone ID Related Accession (Same CDS sequence)	XM_006515863.3 , NM_001163136.1 , XM_006515862.2
Accession Version	NM_001163136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Mus musculus(house mouse)
Product	metastasis-associated in colon cancer protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC138307.2 and AC132596.5. On or before Jul 10, 2009 this sequence version replaced XM_906766.2, XM_138397.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The exon combination of this CCDS representation is inferred based on orthologous transcript alignments, e.g., human AJ313524.1, BC137090.1 or BC144470.1. Partial mouse transcript data also support this representation, e.g., mRNA accession AK028965.1, and RNA-Seq data from the ENCODE project. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGCTAATCA GTGAAAGAGC CTATTGTTGG CCACGAGAAA TTGGTCGAAG TAGATCTGAA 
GGAAATTTGA TTGACATGGA AGCCTCGAAA CGCTCAAAAA ATCAAAATAT TACAGCACGG 
CAAGGGCTAG GCTTGCCTCA CAACTGGCCA GATGCTCCTA CCCTCCATCG TGATAGTGAT 
GCCAAAGCTA CAAATCCATT CTGGAAGGAA CTGTCTGCCT CAAACCCATT TCTGGAGGAC 
ATCACGCAGC TAAGAAACAA CAAAAAGAGA GATAATATGT CTATCTTGAA GGAAGATCCT 
TTTCTTTTTT TAAGACAAAT AGAAACAGAA AATTCTTTTG ACTCCTCTGG TGATGAACTT 
GACATGGATC AGGGGTTTAA GTCATCTTCC TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT 
TCTGAACTCC TGGATGTTTT AGGTGATAGG ACACACACTC ATCAAAGTAT ACATAGCTCT 
GATCAGATCC TAATACAAGA CTTAGAATGG CTACAAAATG ACCGAGAAGC ATATAAAATG 
GCTTGGCTTA GTCAACGTCA GTTGGCTCGC TCTTGCCTAG ATCTGAATAC AATTAATCAG 
AGTCCTGGGT GGGCTCAAAC ACAAATTGCA GAGACCATTG CAGTTTGTAA ATTAAATCAC 
CAAGGAGGGT CAGTCCAATT ACCCGACTCA GACATCACTA TTCATATACC CCAAGGACAT 
ATAGCTGTGG GAGAATTCCA GGAGGTATCT CTGAGAGCAT TTCTGGATCC TCCACCAAAG 
CTGAACCATG ATCTCTCTTG TACTGTAAGC CCTCTCTTGG AAATCAGGTT AAGCAATCTG 
AATACCATGG AAGCTATTTT GTTGGAGATG AAAACTGGAG CAGAAGTAAA AACAGATCCT 
CTGAGCCAAG TCATGACTAA AATGGTGTGT TTGTACAGCC TGGACAGAGA AGGCCCTTTC 
CAAGCATTAA ACACCTGCTA TATCTACAAA GATACCATCC AAGCTAAGCT TCTTGACCTG 
AGCCATGTAA TGTATATAGT CATAGCTGTA CAAGCTACGA TCATTCATCC ACCAGCTGCT 
ACTATTTGGG ATTATATCCA CAAAACCACC TCTATTGGAA TTTATGGACC TAAATATCTC 
CATCCAAGCT TTACTGCTGT TTTCACTGTT TGTGGACACA ACTATGTGCC AGGAAAGTTT 
ACAGTCTCTG ACATTAGGAA GGGTGGAAAA AACACATCTC CAGTCGTATT TCACCTCTGG 
GGGAAGCATT CATTTTCACT TGACAAGCCA AAAGATTTAA TTATTTCAGC TTTTTCGTGT 
GATCCTGATT TTGAAGTAAA GGAAGAGGGA GAAAGAAAAG TAATTGCACA AAGGCAATTG 
GAAGCAGGTG TGGTAGTTCA TCAACAAGTT TTCTTTTCTT CAATTGATCA CAGAGAGATG 
CACTTGTTTG TTTTCCGGGT TCAGGTAAAG ACCCCCAATG AAAAACCAGT GACACAGTTC 
TTTGTCACTT CACCTGATCC ATCCCCAAAT CTAAAAAGGT TCTCATATCT GCCAGGATTT 
TTCCAGAAGA GAGAAGACAA GAGAGTGCCT TTATTGTCGA CAGGACTTGT TAAGTATCCC 
ACTTTCCAAG ATAAAAAATT GAACTTTACC AATTATGGGG TTGCACTGAA GACAGTACTG 
AGGCAAAATA AGATTGACTA TTTGCTTGAA TATTTAAAAG GGGACACAAT TGCCCTTCTT 
GGAGAAGGTA CAGTAAGAGC CATAGGACAG TCCAAAGTGA AAGAATGGTA CGTAGGAGTC 
CTCCGAGGTA AGATCGGACT CGTGCATTGT AAAAATGTCA AAGTGATTTC TAAAGAACAA 
GTGATGTCAA TGTCTGATGG CATTTTGACA ACCAAGAATC TTCTGGAACA AATTGCCTTG 
CCATTTAAAA GTCTGACTTA TATATACTCT GTTGTATTGA CCTTGGTGTC AGAAAAAGTT 
CACGACTGGA AAGTTTTAGC TAATGTCCTG GGTTACTCAC ATCTGGCACA GGAAGATTTT 
AATAGAATTC AAGCAGACAA GGAACCAGAA AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG 
GAAGATTGCC ATGAAGATAG AAATACCAGG AAGTTCCTTT ATGAACTGAT TGTGGCTCTG 
CTAAAAATGG ATTGCCAAGA GTTAGTGGCA CATCTTATCC AAGAGGCTGT TATTCTGACT 
TCAGCTGTGA AACTTGGAAG AAGCTGGAGG GAACTTGCTG AGAAATCTGC AGGACTCACT 
AAGCATCAGA TGCAGGCATA TGAAATTCCT CACCGAGGAA AATCTGGAGA TGTTTCAGCA 
GAGATGATGT GGAAACCTGC CTATGATTTT CTCTATGCAT GGAGTTTTCA CTATGGAAAT 
AGCTACAGAG ATGTGTTACA AGATCTTCAA TCAGCTTTGG ACAGAATGAA AAACCCTGTC 
ACTAAACGGT GGAGAGATTT AACTGGAACT TTAATACTTA TAAATTCTTT AGAGATTCTG 
AGAGTGACTG CTTTCTCTAC TTCTCAGGAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001156608.1
CDS	1..2556
Translation	MLISERAYCWPREIGRSRSEGNLIDMEASKRSKNQNITARQGLGLPHNWPDAPTLHRDSDAKATNPFWKELSASNPFLEDITQLRNNKKRDNMSILKEDPFLFLRQIETENSFDSSGDELDMDQGFKSSSSRKSGRSKSVSELLDVLGDRTHTHQSIHSSDQILIQDLEWLQNDREAYKMAWLSQRQLARSCLDLNTINQSPGWAQTQIAETIAVCKLNHQGGSVQLPDSDITIHIPQGHIAVGEFQEVSLRAFLDPPPKLNHDLSCTVSPLLEIRLSNLNTMEAILLEMKTGAEVKTDPLSQVMTKMVCLYSLDREGPFQALNTCYIYKDTIQAKLLDLSHVMYIVIAVQATIIHPPAATIWDYIHKTTSIGIYGPKYLHPSFTAVFTVCGHNYVPGKFTVSDIRKGGKNTSPVVFHLWGKHSFSLDKPKDLIISAFSCDPDFEVKEEGERKVIAQRQLEAGVVVHQQVFFSSIDHREMHLFVFRVQVKTPNEKPVTQFFVTSPDPSPNLKRFSYLPGFFQKREDKRVPLLSTGLVKYPTFQDKKLNFTNYGVALKTVLRQNKIDYLLEYLKGDTIALLGEGTVRAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMSMSDGILTTKNLLEQIALPFKSLTYIYSVVLTLVSEKVHDWKVLANVLGYSHLAQEDFNRIQADKEPERVSYVVKKLKEDCHEDRNTRKFLYELIVALLKMDCQELVAHLIQEAVILTSAVKLGRSWRELAEKSAGLTKHQMQAYEIPHRGKSGDVSAEMMWKPAYDFLYAWSFHYGNSYRDVLQDLQSALDRMKNPVTKRWRDLTGTLILINSLEILRVTAFSTSQEI

Target ORF information:

RefSeq Version	NM_001163136.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus metastasis associated in colon cancer 1 (Macc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163136.1

ORF Insert Sequence:

ATGCTAATCA GTGAAAGAGC CTATTGTTGG CCACGAGAAA TTGGTCGAAG TAGATCTGAA 
GGAAATTTGA TTGACATGGA AGCCTCGAAA CGCTCAAAAA ATCAAAATAT TACAGCACGG 
CAAGGGCTAG GCTTGCCTCA CAACTGGCCA GATGCTCCTA CCCTCCATCG TGATAGTGAT 
GCCAAAGCTA CAAATCCATT CTGGAAGGAA CTGTCTGCCT CAAACCCATT TCTGGAGGAC 
ATCACGCAGC TAAGAAACAA CAAAAAGAGA GATAATATGT CTATCTTGAA GGAAGATCCT 
TTTCTTTTTT TAAGACAAAT AGAAACAGAA AATTCTTTTG ACTCCTCTGG TGATGAACTT 
GACATGGATC AGGGGTTTAA GTCATCTTCC TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT 
TCTGAACTCC TGGATGTTTT AGGTGATAGG ACACACACTC ATCAAAGTAT ACATAGCTCT 
GATCAGATCC TAATACAAGA CTTAGAATGG CTACAAAATG ACCGAGAAGC ATATAAAATG 
GCTTGGCTTA GTCAACGTCA GTTGGCTCGC TCTTGCCTAG ATCTGAATAC AATTAATCAG 
AGTCCTGGGT GGGCTCAAAC ACAAATTGCA GAGACCATTG CAGTTTGTAA ATTAAATCAC 
CAAGGAGGGT CAGTCCAATT ACCCGACTCA GACATCACTA TTCATATACC CCAAGGACAT 
ATAGCTGTGG GAGAATTCCA GGAGGTATCT CTGAGAGCAT TTCTGGATCC TCCACCAAAG 
CTGAACCATG ATCTCTCTTG TACTGTAAGC CCTCTCTTGG AAATCAGGTT AAGCAATCTG 
AATACCATGG AAGCTATTTT GTTGGAGATG AAAACTGGAG CAGAAGTAAA AACAGATCCT 
CTGAGCCAAG TCATGACTAA AATGGTGTGT TTGTACAGCC TGGACAGAGA AGGCCCTTTC 
CAAGCATTAA ACACCTGCTA TATCTACAAA GATACCATCC AAGCTAAGCT TCTTGACCTG 
AGCCATGTAA TGTATATAGT CATAGCTGTA CAAGCTACGA TCATTCATCC ACCAGCTGCT 
ACTATTTGGG ATTATATCCA CAAAACCACC TCTATTGGAA TTTATGGACC TAAATATCTC 
CATCCAAGCT TTACTGCTGT TTTCACTGTT TGTGGACACA ACTATGTGCC AGGAAAGTTT 
ACAGTCTCTG ACATTAGGAA GGGTGGAAAA AACACATCTC CAGTCGTATT TCACCTCTGG 
GGGAAGCATT CATTTTCACT TGACAAGCCA AAAGATTTAA TTATTTCAGC TTTTTCGTGT 
GATCCTGATT TTGAAGTAAA GGAAGAGGGA GAAAGAAAAG TAATTGCACA AAGGCAATTG 
GAAGCAGGTG TGGTAGTTCA TCAACAAGTT TTCTTTTCTT CAATTGATCA CAGAGAGATG 
CACTTGTTTG TTTTCCGGGT TCAGGTAAAG ACCCCCAATG AAAAACCAGT GACACAGTTC 
TTTGTCACTT CACCTGATCC ATCCCCAAAT CTAAAAAGGT TCTCATATCT GCCAGGATTT 
TTCCAGAAGA GAGAAGACAA GAGAGTGCCT TTATTGTCGA CAGGACTTGT TAAGTATCCC 
ACTTTCCAAG ATAAAAAATT GAACTTTACC AATTATGGGG TTGCACTGAA GACAGTACTG 
AGGCAAAATA AGATTGACTA TTTGCTTGAA TATTTAAAAG GGGACACAAT TGCCCTTCTT 
GGAGAAGGTA CAGTAAGAGC CATAGGACAG TCCAAAGTGA AAGAATGGTA CGTAGGAGTC 
CTCCGAGGTA AGATCGGACT CGTGCATTGT AAAAATGTCA AAGTGATTTC TAAAGAACAA 
GTGATGTCAA TGTCTGATGG CATTTTGACA ACCAAGAATC TTCTGGAACA AATTGCCTTG 
CCATTTAAAA GTCTGACTTA TATATACTCT GTTGTATTGA CCTTGGTGTC AGAAAAAGTT 
CACGACTGGA AAGTTTTAGC TAATGTCCTG GGTTACTCAC ATCTGGCACA GGAAGATTTT 
AATAGAATTC AAGCAGACAA GGAACCAGAA AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG 
GAAGATTGCC ATGAAGATAG AAATACCAGG AAGTTCCTTT ATGAACTGAT TGTGGCTCTG 
CTAAAAATGG ATTGCCAAGA GTTAGTGGCA CATCTTATCC AAGAGGCTGT TATTCTGACT 
TCAGCTGTGA AACTTGGAAG AAGCTGGAGG GAACTTGCTG AGAAATCTGC AGGACTCACT 
AAGCATCAGA TGCAGGCATA TGAAATTCCT CACCGAGGAA AATCTGGAGA TGTTTCAGCA 
GAGATGATGT GGAAACCTGC CTATGATTTT CTCTATGCAT GGAGTTTTCA CTATGGAAAT 
AGCTACAGAG ATGTGTTACA AGATCTTCAA TCAGCTTTGG ACAGAATGAA AAACCCTGTC 
ACTAAACGGT GGAGAGATTT AACTGGAACT TTAATACTTA TAAATTCTTT AGAGATTCTG 
AGAGTGACTG CTTTCTCTAC TTCTCAGGAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Macc1 ( XM_006515863.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515926.1 Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu08749
Clone ID Related Accession (Same CDS sequence)	XM_006515863.3 , NM_001163136.1 , XM_006515862.2
Accession Version	XM_006515863.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	metastasis-associated in colon cancer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515863.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAATCA GTGAAAGAGC CTATTGTTGG CCACGAGAAA TTGGTCGAAG TAGATCTGAA 
GGAAATTTGA TTGACATGGA AGCCTCGAAA CGCTCAAAAA ATCAAAATAT TACAGCACGG 
CAAGGGCTAG GCTTGCCTCA CAACTGGCCA GATGCTCCTA CCCTCCATCG TGATAGTGAT 
GCCAAAGCTA CAAATCCATT CTGGAAGGAA CTGTCTGCCT CAAACCCATT TCTGGAGGAC 
ATCACGCAGC TAAGAAACAA CAAAAAGAGA GATAATATGT CTATCTTGAA GGAAGATCCT 
TTTCTTTTTT TAAGACAAAT AGAAACAGAA AATTCTTTTG ACTCCTCTGG TGATGAACTT 
GACATGGATC AGGGGTTTAA GTCATCTTCC TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT 
TCTGAACTCC TGGATGTTTT AGGTGATAGG ACACACACTC ATCAAAGTAT ACATAGCTCT 
GATCAGATCC TAATACAAGA CTTAGAATGG CTACAAAATG ACCGAGAAGC ATATAAAATG 
GCTTGGCTTA GTCAACGTCA GTTGGCTCGC TCTTGCCTAG ATCTGAATAC AATTAATCAG 
AGTCCTGGGT GGGCTCAAAC ACAAATTGCA GAGACCATTG CAGTTTGTAA ATTAAATCAC 
CAAGGAGGGT CAGTCCAATT ACCCGACTCA GACATCACTA TTCATATACC CCAAGGACAT 
ATAGCTGTGG GAGAATTCCA GGAGGTATCT CTGAGAGCAT TTCTGGATCC TCCACCAAAG 
CTGAACCATG ATCTCTCTTG TACTGTAAGC CCTCTCTTGG AAATCAGGTT AAGCAATCTG 
AATACCATGG AAGCTATTTT GTTGGAGATG AAAACTGGAG CAGAAGTAAA AACAGATCCT 
CTGAGCCAAG TCATGACTAA AATGGTGTGT TTGTACAGCC TGGACAGAGA AGGCCCTTTC 
CAAGCATTAA ACACCTGCTA TATCTACAAA GATACCATCC AAGCTAAGCT TCTTGACCTG 
AGCCATGTAA TGTATATAGT CATAGCTGTA CAAGCTACGA TCATTCATCC ACCAGCTGCT 
ACTATTTGGG ATTATATCCA CAAAACCACC TCTATTGGAA TTTATGGACC TAAATATCTC 
CATCCAAGCT TTACTGCTGT TTTCACTGTT TGTGGACACA ACTATGTGCC AGGAAAGTTT 
ACAGTCTCTG ACATTAGGAA GGGTGGAAAA AACACATCTC CAGTCGTATT TCACCTCTGG 
GGGAAGCATT CATTTTCACT TGACAAGCCA AAAGATTTAA TTATTTCAGC TTTTTCGTGT 
GATCCTGATT TTGAAGTAAA GGAAGAGGGA GAAAGAAAAG TAATTGCACA AAGGCAATTG 
GAAGCAGGTG TGGTAGTTCA TCAACAAGTT TTCTTTTCTT CAATTGATCA CAGAGAGATG 
CACTTGTTTG TTTTCCGGGT TCAGGTAAAG ACCCCCAATG AAAAACCAGT GACACAGTTC 
TTTGTCACTT CACCTGATCC ATCCCCAAAT CTAAAAAGGT TCTCATATCT GCCAGGATTT 
TTCCAGAAGA GAGAAGACAA GAGAGTGCCT TTATTGTCGA CAGGACTTGT TAAGTATCCC 
ACTTTCCAAG ATAAAAAATT GAACTTTACC AATTATGGGG TTGCACTGAA GACAGTACTG 
AGGCAAAATA AGATTGACTA TTTGCTTGAA TATTTAAAAG GGGACACAAT TGCCCTTCTT 
GGAGAAGGTA CAGTAAGAGC CATAGGACAG TCCAAAGTGA AAGAATGGTA CGTAGGAGTC 
CTCCGAGGTA AGATCGGACT CGTGCATTGT AAAAATGTCA AAGTGATTTC TAAAGAACAA 
GTGATGTCAA TGTCTGATGG CATTTTGACA ACCAAGAATC TTCTGGAACA AATTGCCTTG 
CCATTTAAAA GTCTGACTTA TATATACTCT GTTGTATTGA CCTTGGTGTC AGAAAAAGTT 
CACGACTGGA AAGTTTTAGC TAATGTCCTG GGTTACTCAC ATCTGGCACA GGAAGATTTT 
AATAGAATTC AAGCAGACAA GGAACCAGAA AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG 
GAAGATTGCC ATGAAGATAG AAATACCAGG AAGTTCCTTT ATGAACTGAT TGTGGCTCTG 
CTAAAAATGG ATTGCCAAGA GTTAGTGGCA CATCTTATCC AAGAGGCTGT TATTCTGACT 
TCAGCTGTGA AACTTGGAAG AAGCTGGAGG GAACTTGCTG AGAAATCTGC AGGACTCACT 
AAGCATCAGA TGCAGGCATA TGAAATTCCT CACCGAGGAA AATCTGGAGA TGTTTCAGCA 
GAGATGATGT GGAAACCTGC CTATGATTTT CTCTATGCAT GGAGTTTTCA CTATGGAAAT 
AGCTACAGAG ATGTGTTACA AGATCTTCAA TCAGCTTTGG ACAGAATGAA AAACCCTGTC 
ACTAAACGGT GGAGAGATTT AACTGGAACT TTAATACTTA TAAATTCTTT AGAGATTCTG 
AGAGTGACTG CTTTCTCTAC TTCTCAGGAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515926.1
CDS	409..2964
Translation	MLISERAYCWPREIGRSRSEGNLIDMEASKRSKNQNITARQGLGLPHNWPDAPTLHRDSDAKATNPFWKELSASNPFLEDITQLRNNKKRDNMSILKEDPFLFLRQIETENSFDSSGDELDMDQGFKSSSSRKSGRSKSVSELLDVLGDRTHTHQSIHSSDQILIQDLEWLQNDREAYKMAWLSQRQLARSCLDLNTINQSPGWAQTQIAETIAVCKLNHQGGSVQLPDSDITIHIPQGHIAVGEFQEVSLRAFLDPPPKLNHDLSCTVSPLLEIRLSNLNTMEAILLEMKTGAEVKTDPLSQVMTKMVCLYSLDREGPFQALNTCYIYKDTIQAKLLDLSHVMYIVIAVQATIIHPPAATIWDYIHKTTSIGIYGPKYLHPSFTAVFTVCGHNYVPGKFTVSDIRKGGKNTSPVVFHLWGKHSFSLDKPKDLIISAFSCDPDFEVKEEGERKVIAQRQLEAGVVVHQQVFFSSIDHREMHLFVFRVQVKTPNEKPVTQFFVTSPDPSPNLKRFSYLPGFFQKREDKRVPLLSTGLVKYPTFQDKKLNFTNYGVALKTVLRQNKIDYLLEYLKGDTIALLGEGTVRAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMSMSDGILTTKNLLEQIALPFKSLTYIYSVVLTLVSEKVHDWKVLANVLGYSHLAQEDFNRIQADKEPERVSYVVKKLKEDCHEDRNTRKFLYELIVALLKMDCQELVAHLIQEAVILTSAVKLGRSWRELAEKSAGLTKHQMQAYEIPHRGKSGDVSAEMMWKPAYDFLYAWSFHYGNSYRDVLQDLQSALDRMKNPVTKRWRDLTGTLILINSLEILRVTAFSTSQEI

Target ORF information:

RefSeq Version	XM_006515863.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515863.3

ORF Insert Sequence:

ATGCTAATCA GTGAAAGAGC CTATTGTTGG CCACGAGAAA TTGGTCGAAG TAGATCTGAA 
GGAAATTTGA TTGACATGGA AGCCTCGAAA CGCTCAAAAA ATCAAAATAT TACAGCACGG 
CAAGGGCTAG GCTTGCCTCA CAACTGGCCA GATGCTCCTA CCCTCCATCG TGATAGTGAT 
GCCAAAGCTA CAAATCCATT CTGGAAGGAA CTGTCTGCCT CAAACCCATT TCTGGAGGAC 
ATCACGCAGC TAAGAAACAA CAAAAAGAGA GATAATATGT CTATCTTGAA GGAAGATCCT 
TTTCTTTTTT TAAGACAAAT AGAAACAGAA AATTCTTTTG ACTCCTCTGG TGATGAACTT 
GACATGGATC AGGGGTTTAA GTCATCTTCC TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT 
TCTGAACTCC TGGATGTTTT AGGTGATAGG ACACACACTC ATCAAAGTAT ACATAGCTCT 
GATCAGATCC TAATACAAGA CTTAGAATGG CTACAAAATG ACCGAGAAGC ATATAAAATG 
GCTTGGCTTA GTCAACGTCA GTTGGCTCGC TCTTGCCTAG ATCTGAATAC AATTAATCAG 
AGTCCTGGGT GGGCTCAAAC ACAAATTGCA GAGACCATTG CAGTTTGTAA ATTAAATCAC 
CAAGGAGGGT CAGTCCAATT ACCCGACTCA GACATCACTA TTCATATACC CCAAGGACAT 
ATAGCTGTGG GAGAATTCCA GGAGGTATCT CTGAGAGCAT TTCTGGATCC TCCACCAAAG 
CTGAACCATG ATCTCTCTTG TACTGTAAGC CCTCTCTTGG AAATCAGGTT AAGCAATCTG 
AATACCATGG AAGCTATTTT GTTGGAGATG AAAACTGGAG CAGAAGTAAA AACAGATCCT 
CTGAGCCAAG TCATGACTAA AATGGTGTGT TTGTACAGCC TGGACAGAGA AGGCCCTTTC 
CAAGCATTAA ACACCTGCTA TATCTACAAA GATACCATCC AAGCTAAGCT TCTTGACCTG 
AGCCATGTAA TGTATATAGT CATAGCTGTA CAAGCTACGA TCATTCATCC ACCAGCTGCT 
ACTATTTGGG ATTATATCCA CAAAACCACC TCTATTGGAA TTTATGGACC TAAATATCTC 
CATCCAAGCT TTACTGCTGT TTTCACTGTT TGTGGACACA ACTATGTGCC AGGAAAGTTT 
ACAGTCTCTG ACATTAGGAA GGGTGGAAAA AACACATCTC CAGTCGTATT TCACCTCTGG 
GGGAAGCATT CATTTTCACT TGACAAGCCA AAAGATTTAA TTATTTCAGC TTTTTCGTGT 
GATCCTGATT TTGAAGTAAA GGAAGAGGGA GAAAGAAAAG TAATTGCACA AAGGCAATTG 
GAAGCAGGTG TGGTAGTTCA TCAACAAGTT TTCTTTTCTT CAATTGATCA CAGAGAGATG 
CACTTGTTTG TTTTCCGGGT TCAGGTAAAG ACCCCCAATG AAAAACCAGT GACACAGTTC 
TTTGTCACTT CACCTGATCC ATCCCCAAAT CTAAAAAGGT TCTCATATCT GCCAGGATTT 
TTCCAGAAGA GAGAAGACAA GAGAGTGCCT TTATTGTCGA CAGGACTTGT TAAGTATCCC 
ACTTTCCAAG ATAAAAAATT GAACTTTACC AATTATGGGG TTGCACTGAA GACAGTACTG 
AGGCAAAATA AGATTGACTA TTTGCTTGAA TATTTAAAAG GGGACACAAT TGCCCTTCTT 
GGAGAAGGTA CAGTAAGAGC CATAGGACAG TCCAAAGTGA AAGAATGGTA CGTAGGAGTC 
CTCCGAGGTA AGATCGGACT CGTGCATTGT AAAAATGTCA AAGTGATTTC TAAAGAACAA 
GTGATGTCAA TGTCTGATGG CATTTTGACA ACCAAGAATC TTCTGGAACA AATTGCCTTG 
CCATTTAAAA GTCTGACTTA TATATACTCT GTTGTATTGA CCTTGGTGTC AGAAAAAGTT 
CACGACTGGA AAGTTTTAGC TAATGTCCTG GGTTACTCAC ATCTGGCACA GGAAGATTTT 
AATAGAATTC AAGCAGACAA GGAACCAGAA AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG 
GAAGATTGCC ATGAAGATAG AAATACCAGG AAGTTCCTTT ATGAACTGAT TGTGGCTCTG 
CTAAAAATGG ATTGCCAAGA GTTAGTGGCA CATCTTATCC AAGAGGCTGT TATTCTGACT 
TCAGCTGTGA AACTTGGAAG AAGCTGGAGG GAACTTGCTG AGAAATCTGC AGGACTCACT 
AAGCATCAGA TGCAGGCATA TGAAATTCCT CACCGAGGAA AATCTGGAGA TGTTTCAGCA 
GAGATGATGT GGAAACCTGC CTATGATTTT CTCTATGCAT GGAGTTTTCA CTATGGAAAT 
AGCTACAGAG ATGTGTTACA AGATCTTCAA TCAGCTTTGG ACAGAATGAA AAACCCTGTC 
ACTAAACGGT GGAGAGATTT AACTGGAACT TTAATACTTA TAAATTCTTT AGAGATTCTG 
AGAGTGACTG CTTTCTCTAC TTCTCAGGAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Macc1 ( XM_006515862.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515925.1 Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X2, mRNA.

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CloneID	OMu08749
Clone ID Related Accession (Same CDS sequence)	XM_006515863.3 , NM_001163136.1 , XM_006515862.2
Accession Version	XM_006515862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	metastasis-associated in colon cancer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006515862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAATCA GTGAAAGAGC CTATTGTTGG CCACGAGAAA TTGGTCGAAG TAGATCTGAA 
GGAAATTTGA TTGACATGGA AGCCTCGAAA CGCTCAAAAA ATCAAAATAT TACAGCACGG 
CAAGGGCTAG GCTTGCCTCA CAACTGGCCA GATGCTCCTA CCCTCCATCG TGATAGTGAT 
GCCAAAGCTA CAAATCCATT CTGGAAGGAA CTGTCTGCCT CAAACCCATT TCTGGAGGAC 
ATCACGCAGC TAAGAAACAA CAAAAAGAGA GATAATATGT CTATCTTGAA GGAAGATCCT 
TTTCTTTTTT TAAGACAAAT AGAAACAGAA AATTCTTTTG ACTCCTCTGG TGATGAACTT 
GACATGGATC AGGGGTTTAA GTCATCTTCC TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT 
TCTGAACTCC TGGATGTTTT AGGTGATAGG ACACACACTC ATCAAAGTAT ACATAGCTCT 
GATCAGATCC TAATACAAGA CTTAGAATGG CTACAAAATG ACCGAGAAGC ATATAAAATG 
GCTTGGCTTA GTCAACGTCA GTTGGCTCGC TCTTGCCTAG ATCTGAATAC AATTAATCAG 
AGTCCTGGGT GGGCTCAAAC ACAAATTGCA GAGACCATTG CAGTTTGTAA ATTAAATCAC 
CAAGGAGGGT CAGTCCAATT ACCCGACTCA GACATCACTA TTCATATACC CCAAGGACAT 
ATAGCTGTGG GAGAATTCCA GGAGGTATCT CTGAGAGCAT TTCTGGATCC TCCACCAAAG 
CTGAACCATG ATCTCTCTTG TACTGTAAGC CCTCTCTTGG AAATCAGGTT AAGCAATCTG 
AATACCATGG AAGCTATTTT GTTGGAGATG AAAACTGGAG CAGAAGTAAA AACAGATCCT 
CTGAGCCAAG TCATGACTAA AATGGTGTGT TTGTACAGCC TGGACAGAGA AGGCCCTTTC 
CAAGCATTAA ACACCTGCTA TATCTACAAA GATACCATCC AAGCTAAGCT TCTTGACCTG 
AGCCATGTAA TGTATATAGT CATAGCTGTA CAAGCTACGA TCATTCATCC ACCAGCTGCT 
ACTATTTGGG ATTATATCCA CAAAACCACC TCTATTGGAA TTTATGGACC TAAATATCTC 
CATCCAAGCT TTACTGCTGT TTTCACTGTT TGTGGACACA ACTATGTGCC AGGAAAGTTT 
ACAGTCTCTG ACATTAGGAA GGGTGGAAAA AACACATCTC CAGTCGTATT TCACCTCTGG 
GGGAAGCATT CATTTTCACT TGACAAGCCA AAAGATTTAA TTATTTCAGC TTTTTCGTGT 
GATCCTGATT TTGAAGTAAA GGAAGAGGGA GAAAGAAAAG TAATTGCACA AAGGCAATTG 
GAAGCAGGTG TGGTAGTTCA TCAACAAGTT TTCTTTTCTT CAATTGATCA CAGAGAGATG 
CACTTGTTTG TTTTCCGGGT TCAGGTAAAG ACCCCCAATG AAAAACCAGT GACACAGTTC 
TTTGTCACTT CACCTGATCC ATCCCCAAAT CTAAAAAGGT TCTCATATCT GCCAGGATTT 
TTCCAGAAGA GAGAAGACAA GAGAGTGCCT TTATTGTCGA CAGGACTTGT TAAGTATCCC 
ACTTTCCAAG ATAAAAAATT GAACTTTACC AATTATGGGG TTGCACTGAA GACAGTACTG 
AGGCAAAATA AGATTGACTA TTTGCTTGAA TATTTAAAAG GGGACACAAT TGCCCTTCTT 
GGAGAAGGTA CAGTAAGAGC CATAGGACAG TCCAAAGTGA AAGAATGGTA CGTAGGAGTC 
CTCCGAGGTA AGATCGGACT CGTGCATTGT AAAAATGTCA AAGTGATTTC TAAAGAACAA 
GTGATGTCAA TGTCTGATGG CATTTTGACA ACCAAGAATC TTCTGGAACA AATTGCCTTG 
CCATTTAAAA GTCTGACTTA TATATACTCT GTTGTATTGA CCTTGGTGTC AGAAAAAGTT 
CACGACTGGA AAGTTTTAGC TAATGTCCTG GGTTACTCAC ATCTGGCACA GGAAGATTTT 
AATAGAATTC AAGCAGACAA GGAACCAGAA AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG 
GAAGATTGCC ATGAAGATAG AAATACCAGG AAGTTCCTTT ATGAACTGAT TGTGGCTCTG 
CTAAAAATGG ATTGCCAAGA GTTAGTGGCA CATCTTATCC AAGAGGCTGT TATTCTGACT 
TCAGCTGTGA AACTTGGAAG AAGCTGGAGG GAACTTGCTG AGAAATCTGC AGGACTCACT 
AAGCATCAGA TGCAGGCATA TGAAATTCCT CACCGAGGAA AATCTGGAGA TGTTTCAGCA 
GAGATGATGT GGAAACCTGC CTATGATTTT CTCTATGCAT GGAGTTTTCA CTATGGAAAT 
AGCTACAGAG ATGTGTTACA AGATCTTCAA TCAGCTTTGG ACAGAATGAA AAACCCTGTC 
ACTAAACGGT GGAGAGATTT AACTGGAACT TTAATACTTA TAAATTCTTT AGAGATTCTG 
AGAGTGACTG CTTTCTCTAC TTCTCAGGAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515925.1
CDS	351..2906
Translation	MLISERAYCWPREIGRSRSEGNLIDMEASKRSKNQNITARQGLGLPHNWPDAPTLHRDSDAKATNPFWKELSASNPFLEDITQLRNNKKRDNMSILKEDPFLFLRQIETENSFDSSGDELDMDQGFKSSSSRKSGRSKSVSELLDVLGDRTHTHQSIHSSDQILIQDLEWLQNDREAYKMAWLSQRQLARSCLDLNTINQSPGWAQTQIAETIAVCKLNHQGGSVQLPDSDITIHIPQGHIAVGEFQEVSLRAFLDPPPKLNHDLSCTVSPLLEIRLSNLNTMEAILLEMKTGAEVKTDPLSQVMTKMVCLYSLDREGPFQALNTCYIYKDTIQAKLLDLSHVMYIVIAVQATIIHPPAATIWDYIHKTTSIGIYGPKYLHPSFTAVFTVCGHNYVPGKFTVSDIRKGGKNTSPVVFHLWGKHSFSLDKPKDLIISAFSCDPDFEVKEEGERKVIAQRQLEAGVVVHQQVFFSSIDHREMHLFVFRVQVKTPNEKPVTQFFVTSPDPSPNLKRFSYLPGFFQKREDKRVPLLSTGLVKYPTFQDKKLNFTNYGVALKTVLRQNKIDYLLEYLKGDTIALLGEGTVRAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMSMSDGILTTKNLLEQIALPFKSLTYIYSVVLTLVSEKVHDWKVLANVLGYSHLAQEDFNRIQADKEPERVSYVVKKLKEDCHEDRNTRKFLYELIVALLKMDCQELVAHLIQEAVILTSAVKLGRSWRELAEKSAGLTKHQMQAYEIPHRGKSGDVSAEMMWKPAYDFLYAWSFHYGNSYRDVLQDLQSALDRMKNPVTKRWRDLTGTLILINSLEILRVTAFSTSQEI

Target ORF information:

RefSeq Version	XM_006515862.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515862.2

ORF Insert Sequence:

ATGCTAATCA GTGAAAGAGC CTATTGTTGG CCACGAGAAA TTGGTCGAAG TAGATCTGAA 
GGAAATTTGA TTGACATGGA AGCCTCGAAA CGCTCAAAAA ATCAAAATAT TACAGCACGG 
CAAGGGCTAG GCTTGCCTCA CAACTGGCCA GATGCTCCTA CCCTCCATCG TGATAGTGAT 
GCCAAAGCTA CAAATCCATT CTGGAAGGAA CTGTCTGCCT CAAACCCATT TCTGGAGGAC 
ATCACGCAGC TAAGAAACAA CAAAAAGAGA GATAATATGT CTATCTTGAA GGAAGATCCT 
TTTCTTTTTT TAAGACAAAT AGAAACAGAA AATTCTTTTG ACTCCTCTGG TGATGAACTT 
GACATGGATC AGGGGTTTAA GTCATCTTCC TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT 
TCTGAACTCC TGGATGTTTT AGGTGATAGG ACACACACTC ATCAAAGTAT ACATAGCTCT 
GATCAGATCC TAATACAAGA CTTAGAATGG CTACAAAATG ACCGAGAAGC ATATAAAATG 
GCTTGGCTTA GTCAACGTCA GTTGGCTCGC TCTTGCCTAG ATCTGAATAC AATTAATCAG 
AGTCCTGGGT GGGCTCAAAC ACAAATTGCA GAGACCATTG CAGTTTGTAA ATTAAATCAC 
CAAGGAGGGT CAGTCCAATT ACCCGACTCA GACATCACTA TTCATATACC CCAAGGACAT 
ATAGCTGTGG GAGAATTCCA GGAGGTATCT CTGAGAGCAT TTCTGGATCC TCCACCAAAG 
CTGAACCATG ATCTCTCTTG TACTGTAAGC CCTCTCTTGG AAATCAGGTT AAGCAATCTG 
AATACCATGG AAGCTATTTT GTTGGAGATG AAAACTGGAG CAGAAGTAAA AACAGATCCT 
CTGAGCCAAG TCATGACTAA AATGGTGTGT TTGTACAGCC TGGACAGAGA AGGCCCTTTC 
CAAGCATTAA ACACCTGCTA TATCTACAAA GATACCATCC AAGCTAAGCT TCTTGACCTG 
AGCCATGTAA TGTATATAGT CATAGCTGTA CAAGCTACGA TCATTCATCC ACCAGCTGCT 
ACTATTTGGG ATTATATCCA CAAAACCACC TCTATTGGAA TTTATGGACC TAAATATCTC 
CATCCAAGCT TTACTGCTGT TTTCACTGTT TGTGGACACA ACTATGTGCC AGGAAAGTTT 
ACAGTCTCTG ACATTAGGAA GGGTGGAAAA AACACATCTC CAGTCGTATT TCACCTCTGG 
GGGAAGCATT CATTTTCACT TGACAAGCCA AAAGATTTAA TTATTTCAGC TTTTTCGTGT 
GATCCTGATT TTGAAGTAAA GGAAGAGGGA GAAAGAAAAG TAATTGCACA AAGGCAATTG 
GAAGCAGGTG TGGTAGTTCA TCAACAAGTT TTCTTTTCTT CAATTGATCA CAGAGAGATG 
CACTTGTTTG TTTTCCGGGT TCAGGTAAAG ACCCCCAATG AAAAACCAGT GACACAGTTC 
TTTGTCACTT CACCTGATCC ATCCCCAAAT CTAAAAAGGT TCTCATATCT GCCAGGATTT 
TTCCAGAAGA GAGAAGACAA GAGAGTGCCT TTATTGTCGA CAGGACTTGT TAAGTATCCC 
ACTTTCCAAG ATAAAAAATT GAACTTTACC AATTATGGGG TTGCACTGAA GACAGTACTG 
AGGCAAAATA AGATTGACTA TTTGCTTGAA TATTTAAAAG GGGACACAAT TGCCCTTCTT 
GGAGAAGGTA CAGTAAGAGC CATAGGACAG TCCAAAGTGA AAGAATGGTA CGTAGGAGTC 
CTCCGAGGTA AGATCGGACT CGTGCATTGT AAAAATGTCA AAGTGATTTC TAAAGAACAA 
GTGATGTCAA TGTCTGATGG CATTTTGACA ACCAAGAATC TTCTGGAACA AATTGCCTTG 
CCATTTAAAA GTCTGACTTA TATATACTCT GTTGTATTGA CCTTGGTGTC AGAAAAAGTT 
CACGACTGGA AAGTTTTAGC TAATGTCCTG GGTTACTCAC ATCTGGCACA GGAAGATTTT 
AATAGAATTC AAGCAGACAA GGAACCAGAA AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG 
GAAGATTGCC ATGAAGATAG AAATACCAGG AAGTTCCTTT ATGAACTGAT TGTGGCTCTG 
CTAAAAATGG ATTGCCAAGA GTTAGTGGCA CATCTTATCC AAGAGGCTGT TATTCTGACT 
TCAGCTGTGA AACTTGGAAG AAGCTGGAGG GAACTTGCTG AGAAATCTGC AGGACTCACT 
AAGCATCAGA TGCAGGCATA TGAAATTCCT CACCGAGGAA AATCTGGAGA TGTTTCAGCA 
GAGATGATGT GGAAACCTGC CTATGATTTT CTCTATGCAT GGAGTTTTCA CTATGGAAAT 
AGCTACAGAG ATGTGTTACA AGATCTTCAA TCAGCTTTGG ACAGAATGAA AAACCCTGTC 
ACTAAACGGT GGAGAGATTT AACTGGAACT TTAATACTTA TAAATTCTTT AGAGATTCTG 
AGAGTGACTG CTTTCTCTAC TTCTCAGGAA ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Macc1 ( XM_006515861.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515924.1 Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47837
Clone ID Related Accession (Same CDS sequence)	XM_006515861.2 , XM_006515861.3
Accession Version	XM_006515861.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	metastasis-associated in colon cancer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006515861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCTC CAGAGAAAGA TGGTAGCAAA ATGCTAATCA GTGAAAGAGC CTATTGTTGG 
CCACGAGAAA TTGGTCGAAG TAGATCTGAA GGAAATTTGA TTGACATGGA AGCCTCGAAA 
CGCTCAAAAA ATCAAAATAT TACAGCACGG CAAGGGCTAG GCTTGCCTCA CAACTGGCCA 
GATGCTCCTA CCCTCCATCG TGATAGTGAT GCCAAAGCTA CAAATCCATT CTGGAAGGAA 
CTGTCTGCCT CAAACCCATT TCTGGAGGAC ATCACGCAGC TAAGAAACAA CAAAAAGAGA 
GATAATATGT CTATCTTGAA GGAAGATCCT TTTCTTTTTT TAAGACAAAT AGAAACAGAA 
AATTCTTTTG ACTCCTCTGG TGATGAACTT GACATGGATC AGGGGTTTAA GTCATCTTCC 
TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT TCTGAACTCC TGGATGTTTT AGGTGATAGG 
ACACACACTC ATCAAAGTAT ACATAGCTCT GATCAGATCC TAATACAAGA CTTAGAATGG 
CTACAAAATG ACCGAGAAGC ATATAAAATG GCTTGGCTTA GTCAACGTCA GTTGGCTCGC 
TCTTGCCTAG ATCTGAATAC AATTAATCAG AGTCCTGGGT GGGCTCAAAC ACAAATTGCA 
GAGACCATTG CAGTTTGTAA ATTAAATCAC CAAGGAGGGT CAGTCCAATT ACCCGACTCA 
GACATCACTA TTCATATACC CCAAGGACAT ATAGCTGTGG GAGAATTCCA GGAGGTATCT 
CTGAGAGCAT TTCTGGATCC TCCACCAAAG CTGAACCATG ATCTCTCTTG TACTGTAAGC 
CCTCTCTTGG AAATCAGGTT AAGCAATCTG AATACCATGG AAGCTATTTT GTTGGAGATG 
AAAACTGGAG CAGAAGTAAA AACAGATCCT CTGAGCCAAG TCATGACTAA AATGGTGTGT 
TTGTACAGCC TGGACAGAGA AGGCCCTTTC CAAGCATTAA ACACCTGCTA TATCTACAAA 
GATACCATCC AAGCTAAGCT TCTTGACCTG AGCCATGTAA TGTATATAGT CATAGCTGTA 
CAAGCTACGA TCATTCATCC ACCAGCTGCT ACTATTTGGG ATTATATCCA CAAAACCACC 
TCTATTGGAA TTTATGGACC TAAATATCTC CATCCAAGCT TTACTGCTGT TTTCACTGTT 
TGTGGACACA ACTATGTGCC AGGAAAGTTT ACAGTCTCTG ACATTAGGAA GGGTGGAAAA 
AACACATCTC CAGTCGTATT TCACCTCTGG GGGAAGCATT CATTTTCACT TGACAAGCCA 
AAAGATTTAA TTATTTCAGC TTTTTCGTGT GATCCTGATT TTGAAGTAAA GGAAGAGGGA 
GAAAGAAAAG TAATTGCACA AAGGCAATTG GAAGCAGGTG TGGTAGTTCA TCAACAAGTT 
TTCTTTTCTT CAATTGATCA CAGAGAGATG CACTTGTTTG TTTTCCGGGT TCAGGTAAAG 
ACCCCCAATG AAAAACCAGT GACACAGTTC TTTGTCACTT CACCTGATCC ATCCCCAAAT 
CTAAAAAGGT TCTCATATCT GCCAGGATTT TTCCAGAAGA GAGAAGACAA GAGAGTGCCT 
TTATTGTCGA CAGGACTTGT TAAGTATCCC ACTTTCCAAG ATAAAAAATT GAACTTTACC 
AATTATGGGG TTGCACTGAA GACAGTACTG AGGCAAAATA AGATTGACTA TTTGCTTGAA 
TATTTAAAAG GGGACACAAT TGCCCTTCTT GGAGAAGGTA CAGTAAGAGC CATAGGACAG 
TCCAAAGTGA AAGAATGGTA CGTAGGAGTC CTCCGAGGTA AGATCGGACT CGTGCATTGT 
AAAAATGTCA AAGTGATTTC TAAAGAACAA GTGATGTCAA TGTCTGATGG CATTTTGACA 
ACCAAGAATC TTCTGGAACA AATTGCCTTG CCATTTAAAA GTCTGACTTA TATATACTCT 
GTTGTATTGA CCTTGGTGTC AGAAAAAGTT CACGACTGGA AAGTTTTAGC TAATGTCCTG 
GGTTACTCAC ATCTGGCACA GGAAGATTTT AATAGAATTC AAGCAGACAA GGAACCAGAA 
AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG GAAGATTGCC ATGAAGATAG AAATACCAGG 
AAGTTCCTTT ATGAACTGAT TGTGGCTCTG CTAAAAATGG ATTGCCAAGA GTTAGTGGCA 
CATCTTATCC AAGAGGCTGT TATTCTGACT TCAGCTGTGA AACTTGGAAG AAGCTGGAGG 
GAACTTGCTG AGAAATCTGC AGGACTCACT AAGCATCAGA TGCAGGCATA TGAAATTCCT 
CACCGAGGAA AATCTGGAGA TGTTTCAGCA GAGATGATGT GGAAACCTGC CTATGATTTT 
CTCTATGCAT GGAGTTTTCA CTATGGAAAT AGCTACAGAG ATGTGTTACA AGATCTTCAA 
TCAGCTTTGG ACAGAATGAA AAACCCTGTC ACTAAACGGT GGAGAGATTT AACTGGAACT 
TTAATACTTA TAAATTCTTT AGAGATTCTG AGAGTGACTG CTTTCTCTAC TTCTCAGGAA 
ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515924.1
CDS	396..2981
Translation	MISPEKDGSKMLISERAYCWPREIGRSRSEGNLIDMEASKRSKNQNITARQGLGLPHNWPDAPTLHRDSDAKATNPFWKELSASNPFLEDITQLRNNKKRDNMSILKEDPFLFLRQIETENSFDSSGDELDMDQGFKSSSSRKSGRSKSVSELLDVLGDRTHTHQSIHSSDQILIQDLEWLQNDREAYKMAWLSQRQLARSCLDLNTINQSPGWAQTQIAETIAVCKLNHQGGSVQLPDSDITIHIPQGHIAVGEFQEVSLRAFLDPPPKLNHDLSCTVSPLLEIRLSNLNTMEAILLEMKTGAEVKTDPLSQVMTKMVCLYSLDREGPFQALNTCYIYKDTIQAKLLDLSHVMYIVIAVQATIIHPPAATIWDYIHKTTSIGIYGPKYLHPSFTAVFTVCGHNYVPGKFTVSDIRKGGKNTSPVVFHLWGKHSFSLDKPKDLIISAFSCDPDFEVKEEGERKVIAQRQLEAGVVVHQQVFFSSIDHREMHLFVFRVQVKTPNEKPVTQFFVTSPDPSPNLKRFSYLPGFFQKREDKRVPLLSTGLVKYPTFQDKKLNFTNYGVALKTVLRQNKIDYLLEYLKGDTIALLGEGTVRAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMSMSDGILTTKNLLEQIALPFKSLTYIYSVVLTLVSEKVHDWKVLANVLGYSHLAQEDFNRIQADKEPERVSYVVKKLKEDCHEDRNTRKFLYELIVALLKMDCQELVAHLIQEAVILTSAVKLGRSWRELAEKSAGLTKHQMQAYEIPHRGKSGDVSAEMMWKPAYDFLYAWSFHYGNSYRDVLQDLQSALDRMKNPVTKRWRDLTGTLILINSLEILRVTAFSTSQEI

Target ORF information:

RefSeq Version	XM_006515861.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515861.2

ORF Insert Sequence:

ATGATCTCTC CAGAGAAAGA TGGTAGCAAA ATGCTAATCA GTGAAAGAGC CTATTGTTGG 
CCACGAGAAA TTGGTCGAAG TAGATCTGAA GGAAATTTGA TTGACATGGA AGCCTCGAAA 
CGCTCAAAAA ATCAAAATAT TACAGCACGG CAAGGGCTAG GCTTGCCTCA CAACTGGCCA 
GATGCTCCTA CCCTCCATCG TGATAGTGAT GCCAAAGCTA CAAATCCATT CTGGAAGGAA 
CTGTCTGCCT CAAACCCATT TCTGGAGGAC ATCACGCAGC TAAGAAACAA CAAAAAGAGA 
GATAATATGT CTATCTTGAA GGAAGATCCT TTTCTTTTTT TAAGACAAAT AGAAACAGAA 
AATTCTTTTG ACTCCTCTGG TGATGAACTT GACATGGATC AGGGGTTTAA GTCATCTTCC 
TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT TCTGAACTCC TGGATGTTTT AGGTGATAGG 
ACACACACTC ATCAAAGTAT ACATAGCTCT GATCAGATCC TAATACAAGA CTTAGAATGG 
CTACAAAATG ACCGAGAAGC ATATAAAATG GCTTGGCTTA GTCAACGTCA GTTGGCTCGC 
TCTTGCCTAG ATCTGAATAC AATTAATCAG AGTCCTGGGT GGGCTCAAAC ACAAATTGCA 
GAGACCATTG CAGTTTGTAA ATTAAATCAC CAAGGAGGGT CAGTCCAATT ACCCGACTCA 
GACATCACTA TTCATATACC CCAAGGACAT ATAGCTGTGG GAGAATTCCA GGAGGTATCT 
CTGAGAGCAT TTCTGGATCC TCCACCAAAG CTGAACCATG ATCTCTCTTG TACTGTAAGC 
CCTCTCTTGG AAATCAGGTT AAGCAATCTG AATACCATGG AAGCTATTTT GTTGGAGATG 
AAAACTGGAG CAGAAGTAAA AACAGATCCT CTGAGCCAAG TCATGACTAA AATGGTGTGT 
TTGTACAGCC TGGACAGAGA AGGCCCTTTC CAAGCATTAA ACACCTGCTA TATCTACAAA 
GATACCATCC AAGCTAAGCT TCTTGACCTG AGCCATGTAA TGTATATAGT CATAGCTGTA 
CAAGCTACGA TCATTCATCC ACCAGCTGCT ACTATTTGGG ATTATATCCA CAAAACCACC 
TCTATTGGAA TTTATGGACC TAAATATCTC CATCCAAGCT TTACTGCTGT TTTCACTGTT 
TGTGGACACA ACTATGTGCC AGGAAAGTTT ACAGTCTCTG ACATTAGGAA GGGTGGAAAA 
AACACATCTC CAGTCGTATT TCACCTCTGG GGGAAGCATT CATTTTCACT TGACAAGCCA 
AAAGATTTAA TTATTTCAGC TTTTTCGTGT GATCCTGATT TTGAAGTAAA GGAAGAGGGA 
GAAAGAAAAG TAATTGCACA AAGGCAATTG GAAGCAGGTG TGGTAGTTCA TCAACAAGTT 
TTCTTTTCTT CAATTGATCA CAGAGAGATG CACTTGTTTG TTTTCCGGGT TCAGGTAAAG 
ACCCCCAATG AAAAACCAGT GACACAGTTC TTTGTCACTT CACCTGATCC ATCCCCAAAT 
CTAAAAAGGT TCTCATATCT GCCAGGATTT TTCCAGAAGA GAGAAGACAA GAGAGTGCCT 
TTATTGTCGA CAGGACTTGT TAAGTATCCC ACTTTCCAAG ATAAAAAATT GAACTTTACC 
AATTATGGGG TTGCACTGAA GACAGTACTG AGGCAAAATA AGATTGACTA TTTGCTTGAA 
TATTTAAAAG GGGACACAAT TGCCCTTCTT GGAGAAGGTA CAGTAAGAGC CATAGGACAG 
TCCAAAGTGA AAGAATGGTA CGTAGGAGTC CTCCGAGGTA AGATCGGACT CGTGCATTGT 
AAAAATGTCA AAGTGATTTC TAAAGAACAA GTGATGTCAA TGTCTGATGG CATTTTGACA 
ACCAAGAATC TTCTGGAACA AATTGCCTTG CCATTTAAAA GTCTGACTTA TATATACTCT 
GTTGTATTGA CCTTGGTGTC AGAAAAAGTT CACGACTGGA AAGTTTTAGC TAATGTCCTG 
GGTTACTCAC ATCTGGCACA GGAAGATTTT AATAGAATTC AAGCAGACAA GGAACCAGAA 
AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG GAAGATTGCC ATGAAGATAG AAATACCAGG 
AAGTTCCTTT ATGAACTGAT TGTGGCTCTG CTAAAAATGG ATTGCCAAGA GTTAGTGGCA 
CATCTTATCC AAGAGGCTGT TATTCTGACT TCAGCTGTGA AACTTGGAAG AAGCTGGAGG 
GAACTTGCTG AGAAATCTGC AGGACTCACT AAGCATCAGA TGCAGGCATA TGAAATTCCT 
CACCGAGGAA AATCTGGAGA TGTTTCAGCA GAGATGATGT GGAAACCTGC CTATGATTTT 
CTCTATGCAT GGAGTTTTCA CTATGGAAAT AGCTACAGAG ATGTGTTACA AGATCTTCAA 
TCAGCTTTGG ACAGAATGAA AAACCCTGTC ACTAAACGGT GGAGAGATTT AACTGGAACT 
TTAATACTTA TAAATTCTTT AGAGATTCTG AGAGTGACTG CTTTCTCTAC TTCTCAGGAA 
ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Macc1 ( XM_006515861.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515924.1 Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X1, mRNA.

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CloneID	OMu47837
Clone ID Related Accession (Same CDS sequence)	XM_006515861.2 , XM_006515861.3
Accession Version	XM_006515861.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	metastasis-associated in colon cancer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515861.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTCTC CAGAGAAAGA TGGTAGCAAA ATGCTAATCA GTGAAAGAGC CTATTGTTGG 
CCACGAGAAA TTGGTCGAAG TAGATCTGAA GGAAATTTGA TTGACATGGA AGCCTCGAAA 
CGCTCAAAAA ATCAAAATAT TACAGCACGG CAAGGGCTAG GCTTGCCTCA CAACTGGCCA 
GATGCTCCTA CCCTCCATCG TGATAGTGAT GCCAAAGCTA CAAATCCATT CTGGAAGGAA 
CTGTCTGCCT CAAACCCATT TCTGGAGGAC ATCACGCAGC TAAGAAACAA CAAAAAGAGA 
GATAATATGT CTATCTTGAA GGAAGATCCT TTTCTTTTTT TAAGACAAAT AGAAACAGAA 
AATTCTTTTG ACTCCTCTGG TGATGAACTT GACATGGATC AGGGGTTTAA GTCATCTTCC 
TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT TCTGAACTCC TGGATGTTTT AGGTGATAGG 
ACACACACTC ATCAAAGTAT ACATAGCTCT GATCAGATCC TAATACAAGA CTTAGAATGG 
CTACAAAATG ACCGAGAAGC ATATAAAATG GCTTGGCTTA GTCAACGTCA GTTGGCTCGC 
TCTTGCCTAG ATCTGAATAC AATTAATCAG AGTCCTGGGT GGGCTCAAAC ACAAATTGCA 
GAGACCATTG CAGTTTGTAA ATTAAATCAC CAAGGAGGGT CAGTCCAATT ACCCGACTCA 
GACATCACTA TTCATATACC CCAAGGACAT ATAGCTGTGG GAGAATTCCA GGAGGTATCT 
CTGAGAGCAT TTCTGGATCC TCCACCAAAG CTGAACCATG ATCTCTCTTG TACTGTAAGC 
CCTCTCTTGG AAATCAGGTT AAGCAATCTG AATACCATGG AAGCTATTTT GTTGGAGATG 
AAAACTGGAG CAGAAGTAAA AACAGATCCT CTGAGCCAAG TCATGACTAA AATGGTGTGT 
TTGTACAGCC TGGACAGAGA AGGCCCTTTC CAAGCATTAA ACACCTGCTA TATCTACAAA 
GATACCATCC AAGCTAAGCT TCTTGACCTG AGCCATGTAA TGTATATAGT CATAGCTGTA 
CAAGCTACGA TCATTCATCC ACCAGCTGCT ACTATTTGGG ATTATATCCA CAAAACCACC 
TCTATTGGAA TTTATGGACC TAAATATCTC CATCCAAGCT TTACTGCTGT TTTCACTGTT 
TGTGGACACA ACTATGTGCC AGGAAAGTTT ACAGTCTCTG ACATTAGGAA GGGTGGAAAA 
AACACATCTC CAGTCGTATT TCACCTCTGG GGGAAGCATT CATTTTCACT TGACAAGCCA 
AAAGATTTAA TTATTTCAGC TTTTTCGTGT GATCCTGATT TTGAAGTAAA GGAAGAGGGA 
GAAAGAAAAG TAATTGCACA AAGGCAATTG GAAGCAGGTG TGGTAGTTCA TCAACAAGTT 
TTCTTTTCTT CAATTGATCA CAGAGAGATG CACTTGTTTG TTTTCCGGGT TCAGGTAAAG 
ACCCCCAATG AAAAACCAGT GACACAGTTC TTTGTCACTT CACCTGATCC ATCCCCAAAT 
CTAAAAAGGT TCTCATATCT GCCAGGATTT TTCCAGAAGA GAGAAGACAA GAGAGTGCCT 
TTATTGTCGA CAGGACTTGT TAAGTATCCC ACTTTCCAAG ATAAAAAATT GAACTTTACC 
AATTATGGGG TTGCACTGAA GACAGTACTG AGGCAAAATA AGATTGACTA TTTGCTTGAA 
TATTTAAAAG GGGACACAAT TGCCCTTCTT GGAGAAGGTA CAGTAAGAGC CATAGGACAG 
TCCAAAGTGA AAGAATGGTA CGTAGGAGTC CTCCGAGGTA AGATCGGACT CGTGCATTGT 
AAAAATGTCA AAGTGATTTC TAAAGAACAA GTGATGTCAA TGTCTGATGG CATTTTGACA 
ACCAAGAATC TTCTGGAACA AATTGCCTTG CCATTTAAAA GTCTGACTTA TATATACTCT 
GTTGTATTGA CCTTGGTGTC AGAAAAAGTT CACGACTGGA AAGTTTTAGC TAATGTCCTG 
GGTTACTCAC ATCTGGCACA GGAAGATTTT AATAGAATTC AAGCAGACAA GGAACCAGAA 
AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG GAAGATTGCC ATGAAGATAG AAATACCAGG 
AAGTTCCTTT ATGAACTGAT TGTGGCTCTG CTAAAAATGG ATTGCCAAGA GTTAGTGGCA 
CATCTTATCC AAGAGGCTGT TATTCTGACT TCAGCTGTGA AACTTGGAAG AAGCTGGAGG 
GAACTTGCTG AGAAATCTGC AGGACTCACT AAGCATCAGA TGCAGGCATA TGAAATTCCT 
CACCGAGGAA AATCTGGAGA TGTTTCAGCA GAGATGATGT GGAAACCTGC CTATGATTTT 
CTCTATGCAT GGAGTTTTCA CTATGGAAAT AGCTACAGAG ATGTGTTACA AGATCTTCAA 
TCAGCTTTGG ACAGAATGAA AAACCCTGTC ACTAAACGGT GGAGAGATTT AACTGGAACT 
TTAATACTTA TAAATTCTTT AGAGATTCTG AGAGTGACTG CTTTCTCTAC TTCTCAGGAA 
ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515924.1
CDS	397..2982
Translation	MISPEKDGSKMLISERAYCWPREIGRSRSEGNLIDMEASKRSKNQNITARQGLGLPHNWPDAPTLHRDSDAKATNPFWKELSASNPFLEDITQLRNNKKRDNMSILKEDPFLFLRQIETENSFDSSGDELDMDQGFKSSSSRKSGRSKSVSELLDVLGDRTHTHQSIHSSDQILIQDLEWLQNDREAYKMAWLSQRQLARSCLDLNTINQSPGWAQTQIAETIAVCKLNHQGGSVQLPDSDITIHIPQGHIAVGEFQEVSLRAFLDPPPKLNHDLSCTVSPLLEIRLSNLNTMEAILLEMKTGAEVKTDPLSQVMTKMVCLYSLDREGPFQALNTCYIYKDTIQAKLLDLSHVMYIVIAVQATIIHPPAATIWDYIHKTTSIGIYGPKYLHPSFTAVFTVCGHNYVPGKFTVSDIRKGGKNTSPVVFHLWGKHSFSLDKPKDLIISAFSCDPDFEVKEEGERKVIAQRQLEAGVVVHQQVFFSSIDHREMHLFVFRVQVKTPNEKPVTQFFVTSPDPSPNLKRFSYLPGFFQKREDKRVPLLSTGLVKYPTFQDKKLNFTNYGVALKTVLRQNKIDYLLEYLKGDTIALLGEGTVRAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMSMSDGILTTKNLLEQIALPFKSLTYIYSVVLTLVSEKVHDWKVLANVLGYSHLAQEDFNRIQADKEPERVSYVVKKLKEDCHEDRNTRKFLYELIVALLKMDCQELVAHLIQEAVILTSAVKLGRSWRELAEKSAGLTKHQMQAYEIPHRGKSGDVSAEMMWKPAYDFLYAWSFHYGNSYRDVLQDLQSALDRMKNPVTKRWRDLTGTLILINSLEILRVTAFSTSQEI

Target ORF information:

RefSeq Version	XM_006515861.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus metastasis associated in colon cancer 1 (Macc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515861.3

ORF Insert Sequence:

ATGATCTCTC CAGAGAAAGA TGGTAGCAAA ATGCTAATCA GTGAAAGAGC CTATTGTTGG 
CCACGAGAAA TTGGTCGAAG TAGATCTGAA GGAAATTTGA TTGACATGGA AGCCTCGAAA 
CGCTCAAAAA ATCAAAATAT TACAGCACGG CAAGGGCTAG GCTTGCCTCA CAACTGGCCA 
GATGCTCCTA CCCTCCATCG TGATAGTGAT GCCAAAGCTA CAAATCCATT CTGGAAGGAA 
CTGTCTGCCT CAAACCCATT TCTGGAGGAC ATCACGCAGC TAAGAAACAA CAAAAAGAGA 
GATAATATGT CTATCTTGAA GGAAGATCCT TTTCTTTTTT TAAGACAAAT AGAAACAGAA 
AATTCTTTTG ACTCCTCTGG TGATGAACTT GACATGGATC AGGGGTTTAA GTCATCTTCC 
TCTCGGAAGT CAGGAAGGTC TAAGAGTGTT TCTGAACTCC TGGATGTTTT AGGTGATAGG 
ACACACACTC ATCAAAGTAT ACATAGCTCT GATCAGATCC TAATACAAGA CTTAGAATGG 
CTACAAAATG ACCGAGAAGC ATATAAAATG GCTTGGCTTA GTCAACGTCA GTTGGCTCGC 
TCTTGCCTAG ATCTGAATAC AATTAATCAG AGTCCTGGGT GGGCTCAAAC ACAAATTGCA 
GAGACCATTG CAGTTTGTAA ATTAAATCAC CAAGGAGGGT CAGTCCAATT ACCCGACTCA 
GACATCACTA TTCATATACC CCAAGGACAT ATAGCTGTGG GAGAATTCCA GGAGGTATCT 
CTGAGAGCAT TTCTGGATCC TCCACCAAAG CTGAACCATG ATCTCTCTTG TACTGTAAGC 
CCTCTCTTGG AAATCAGGTT AAGCAATCTG AATACCATGG AAGCTATTTT GTTGGAGATG 
AAAACTGGAG CAGAAGTAAA AACAGATCCT CTGAGCCAAG TCATGACTAA AATGGTGTGT 
TTGTACAGCC TGGACAGAGA AGGCCCTTTC CAAGCATTAA ACACCTGCTA TATCTACAAA 
GATACCATCC AAGCTAAGCT TCTTGACCTG AGCCATGTAA TGTATATAGT CATAGCTGTA 
CAAGCTACGA TCATTCATCC ACCAGCTGCT ACTATTTGGG ATTATATCCA CAAAACCACC 
TCTATTGGAA TTTATGGACC TAAATATCTC CATCCAAGCT TTACTGCTGT TTTCACTGTT 
TGTGGACACA ACTATGTGCC AGGAAAGTTT ACAGTCTCTG ACATTAGGAA GGGTGGAAAA 
AACACATCTC CAGTCGTATT TCACCTCTGG GGGAAGCATT CATTTTCACT TGACAAGCCA 
AAAGATTTAA TTATTTCAGC TTTTTCGTGT GATCCTGATT TTGAAGTAAA GGAAGAGGGA 
GAAAGAAAAG TAATTGCACA AAGGCAATTG GAAGCAGGTG TGGTAGTTCA TCAACAAGTT 
TTCTTTTCTT CAATTGATCA CAGAGAGATG CACTTGTTTG TTTTCCGGGT TCAGGTAAAG 
ACCCCCAATG AAAAACCAGT GACACAGTTC TTTGTCACTT CACCTGATCC ATCCCCAAAT 
CTAAAAAGGT TCTCATATCT GCCAGGATTT TTCCAGAAGA GAGAAGACAA GAGAGTGCCT 
TTATTGTCGA CAGGACTTGT TAAGTATCCC ACTTTCCAAG ATAAAAAATT GAACTTTACC 
AATTATGGGG TTGCACTGAA GACAGTACTG AGGCAAAATA AGATTGACTA TTTGCTTGAA 
TATTTAAAAG GGGACACAAT TGCCCTTCTT GGAGAAGGTA CAGTAAGAGC CATAGGACAG 
TCCAAAGTGA AAGAATGGTA CGTAGGAGTC CTCCGAGGTA AGATCGGACT CGTGCATTGT 
AAAAATGTCA AAGTGATTTC TAAAGAACAA GTGATGTCAA TGTCTGATGG CATTTTGACA 
ACCAAGAATC TTCTGGAACA AATTGCCTTG CCATTTAAAA GTCTGACTTA TATATACTCT 
GTTGTATTGA CCTTGGTGTC AGAAAAAGTT CACGACTGGA AAGTTTTAGC TAATGTCCTG 
GGTTACTCAC ATCTGGCACA GGAAGATTTT AATAGAATTC AAGCAGACAA GGAACCAGAA 
AGGGTTTCTT ACGTTGTCAA GAAGTTAAAG GAAGATTGCC ATGAAGATAG AAATACCAGG 
AAGTTCCTTT ATGAACTGAT TGTGGCTCTG CTAAAAATGG ATTGCCAAGA GTTAGTGGCA 
CATCTTATCC AAGAGGCTGT TATTCTGACT TCAGCTGTGA AACTTGGAAG AAGCTGGAGG 
GAACTTGCTG AGAAATCTGC AGGACTCACT AAGCATCAGA TGCAGGCATA TGAAATTCCT 
CACCGAGGAA AATCTGGAGA TGTTTCAGCA GAGATGATGT GGAAACCTGC CTATGATTTT 
CTCTATGCAT GGAGTTTTCA CTATGGAAAT AGCTACAGAG ATGTGTTACA AGATCTTCAA 
TCAGCTTTGG ACAGAATGAA AAACCCTGTC ACTAAACGGT GGAGAGATTT AACTGGAACT 
TTAATACTTA TAAATTCTTT AGAGATTCTG AGAGTGACTG CTTTCTCTAC TTCTCAGGAA 
ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Metastasis-associated in colon cancer-1 upregulates vascular endothelial growth factor-C/D to promote lymphangiogenesis in human gastric cancer.
Cancer letters357(1)242-53(2015 Feb)
Sun L,Duan J,Jiang Y,Wang L,Huang N,Lin L,Liao Y,Liao W

Analysis of HGF, MACC1, C-met and apoptosis-related genes in cervical carcinoma mice.
Molecular biology reports41(3)1247-56(2014 Mar)
Chen XP,Ren XP,Lan JY,Chen YG,Shen ZJ

In vivo imaging of colorectal cancer growth and metastasis by targeting MACC1 with shRNA in xenografted mice.
Clinical & experimental metastasis29(6)573-83(2012 Aug)
Pichorner A,Sack U,Kobelt D,Kelch I,Arlt F,Smith J,Walther W,Schlag PM,Stein U

MACC1, a newly identified key regulator of HGF-MET signaling, predicts colon cancer metastasis.
Nature medicine15(1)59-67(2009 Jan)
Stein U,Walther W,Arlt F,Schwabe H,Smith J,Fichtner I,Birchmeier W,Schlag PM

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Macc1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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