Phgdh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phgdh
Entrez Gene ID	236539
Full Name	3-phosphoglycerate dehydrogenase
Synonyms	3-PGDH,3PGDH,4930479N23,A10,PGAD,PGD,PGDH,SERA
General protein information	Preferred Names 3-phosphoglycerate dehydrogenase Names D-3-phosphoglycerate dehydrogenase 3-phosphoglycerate dehyrogenase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_016966.3	NP_058662.2	D-3-phosphoglycerate dehydrogenase

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	EDA9	NP_195146.1
Pan troglodytes (chimpanzee)	PHGDH	NP_001092041.1
Canis lupus familiaris (dog)	LOC607890	XP_849835.1
Gallus gallus (chicken)	PHGDH	XP_422226.2
Drosophila melanogaster (fruit fly)	CG6287	NP_609496.1
Caenorhabditis elegans (roundworm)	CELE_C31C9.2	NP_496868.1
Oryza sativa (rice)	Os04g0650800	NP_001054094.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008849	XP_319591.2
Xenopus tropicalis (tropical clawed frog)	phgdh	NP_001015929.1
Homo sapiens (human)	PHGDH	NP_006614.2
Bos taurus (cattle)	PHGDH	NP_001030189.1
Rattus norvegicus (Norway rat)	Phgdh	NP_113808.1
Magnaporthe oryzae (rice blast fungus)	MGG_07207	XP_003715419.1
	PHGDH	XP_001114128.1
Canis lupus familiaris (dog)	PHGDH	XP_003639659.1
Canis lupus familiaris (dog)	LOC608055	XP_850035.1
Mus musculus (house mouse)	Phgdh	NP_058662.2
Danio rerio (zebrafish)	phgdh	NP_955871.1

Gene Ontology
Bibliography

Related articles in PubMed

Steroid receptor coactivators 1 and 2 mediate fetal-to-maternal signaling that initiates parturition.
Gao L, Rabbitt EH, Condon JC, Renthal NE, Johnston JM, Mitsche MA, Chambon P, Xu J, O'Malley BW, Mendelson CR
The Journal of clinical investigation125(7)2808-24(2015 Jul)

Requirement of the expression of 3-phosphoglycerate dehydrogenase for traversing S phase in murine T lymphocytes following immobilized anti-CD3 activation.
Jun do Y, Taub D, Chrest FJ, Kim YH
Cellular immunology287(2)78-85(2014 Feb)

D-serine in glia and neurons derives from 3-phosphoglycerate dehydrogenase.
Ehmsen JT, Ma TM, Sason H, Rosenberg D, Ogo T, Furuya S, Snyder SH, Wolosker H
The Journal of neuroscience : the official journal of the Society for Neuroscience33(30)12464-9(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate that the PHGDH gene expression, dictated by IL-2R signaling, is a crucial event for DNA synthesis during S phase of activated T cells.
Title: Requirement of the expression of 3-phosphoglycerate dehydrogenase for traversing S phase in murine T lymphocytes following immobilized anti-CD3 activation.

Using transgenic mice expressing enhanced GFP under the Srr promoter and mice with targeted deletion of Srr or 3-Phosphoglycerate dehydrogenase, we demonstrate predominantly neuronal sources of d-serine dependent on astrocytic supply of l-serine.
Title: D-serine in glia and neurons derives from 3-phosphoglycerate dehydrogenase.

Elevated levels of 4E-BP1 may be involved in a mechanism to arrest brain development in Phgdh knock out embryos.
Title: L-serine deficiency caused by genetic Phgdh deletion leads to robust induction of 4E-BP1 and subsequent repression of translation initiation in the developing central nervous system.

Phgdh deletion reveals a pivotal role for L-serine biosynthesis in controlling the level of brain D-serine, an N-methyl-D-aspartate receptor co-agonist
Title: Brain-specific Phgdh deletion reveals a pivotal role for L-serine biosynthesis in controlling the level of D-serine, an N-methyl-D-aspartate receptor co-agonist, in adult brain.

Data suggest expression of Phgdh in endometrium is regulated by Hoxa10.
Title: 3-Phosphoglycerate dehydrogenase expression is regulated by HOXA10 in murine endometrium and human endometrial cells.

The following Phgdh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phgdh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16633	NM_016966.3 Latest version!	Mus musculus 3-phosphoglycerate dehydrogenase (Phgdh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$314.30 $449.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phgdh ( NM_016966.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058662.2 Mus musculus 3-phosphoglycerate dehydrogenase (Phgdh), mRNA.

Price & Availability↑

CloneID	OMu16633
Clone ID Related Accession (Same CDS sequence)	NM_016966.3
Accession Version	NM_016966.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	D-3-phosphoglycerate dehydrogenase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY290664.1, AK076815.1 and AW545294.1. On or before May 4, 2006 this sequence version replaced XM_001005889.1, NM_016966.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK076815.1, BC110673.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTTCG CAAATCTGCG CAAAGTACTC ATCAGTGACA GCCTGGACCC CTGCTGCCGG 
AAGATCCTGC AAGATGGAGG GCTGCAGGTG GTGGAGAAGC AGAACTTGAG CAAGGAGGAG 
CTGATAGCTG AACTCCAGGA CTGTGAAGGC CTCATTGTCC GGTCTGCTAC TAAGGTCACT 
GCTGATGTCA TCAATGCAGC AGAGAAGCTC CAGGTGGTGG GCAGGGCTGG CACAGGTGTG 
GACAATGTGG ATCTGGAGGC TGCCACGAGG AAGGGCATCC TAGTCATGAA CACCCCCAAC 
GGGAACAGCC TCAGTGCTGC GGAGCTCACC TGTGGGATGA TCATGTGCCT GGCCAGGCAG 
ATTCCCCAGG CAACAGCTTC GATGAAAGAT GGCAAATGGG ACCGGAAGAA GTTCATGGGG 
ACAGAACTGA ACGGGAAGAC ACTGGGAATT CTTGGCCTCG GCAGAATTGG AAGAGAGGTG 
GCCACCCGAA TGCAATCCTT TGGAATGAAG ACTGTAGGCT ATGACCCCAT CATCTCTCCT 
GAAGTCGCCG CCTCCTTTGG TGTTCAGCAG CTGCCGCTGG AGGAGATCTG GCCTCTCTGT 
GACTTTATAA CTGTCCATAC CCCACTCCTG CCCTCTACCA CAGGCTTGCT GAATGACAGC 
ACCTTTGCTC AGTGCAAGAA AGGTGTGCGA GTAGTGAACT GTGCTCGAGG AGGCATTGTG 
GATGAAGGTG CCCTGCTGCG TGCCCTGCAG TCTGGTCAGT GTGCTGGGGC TGCACTGGAT 
GTGTTTACAG AAGAGCCACC ACGGGACCGG GCCTTAGTGG ACCACGAGAA TGTCATCAGC 
TGTCCCCACC TGGGTGCCAG CACCAAGGAA GCCCAGAGCC GCTGTGGGGA GGAAATCGCA 
GTCCAGTTTG TGGACATGGT GAAGGGGAAA TCTCTAACAG GCGTTGTGAA CGCCCAGGCC 
CTCACCAGTG CCTTCTCTCC ACACACCAAG CCTTGGATTG GTCTGGCAGA AGCAATGGGC 
ACGCTGATGC ACGCCTGGGC TGGCTCCCCT AAAGGGACCA TCCAGGTGGT TACACAAGGA 
ACATCTCTGA AGAATGCTGG GACCTGCCTG AGCCCTGCAG TCATTGTCGG CCTTCTGAGA 
GAAGCATCTA AGCAGGCAGA CGTGAACTTG GTGAACGCTA AGCTACTGGT GAAAGAGGCT 
GGCCTCAATG TCACCACCTC CCACAACCCT GGGGTTCCAG GGGAGCAGGG TAGCGGGGAA 
TGCCTCCTGA CTGTGGCCCT GGCAGGTGCT CCCTACCAAG CCGTGGGCTT GGTCCAGGGC 
ACCACACCAA TGCTGCAGAT GCTCAACGGA GCTGTCTTCA GACCAGAGGT GCCACTACGC 
AGGGGCCAAC CCCTGCTCGT ATTCCGGGCT CAGCCCTCCG ACCCTGGGAT GCTGCCCACT 
ATGATTGGCC TCCTGGCAGA GGCGGGTGTA CAGCTGCTGT CCTACCAAAC CTCCATGGTG 
TCTGACGGAG AGCCCTGGCA CGTCATGGGC CTGTCCTCCC TGCTGCCCAG CCTGGAAACA 
TGGAAGCAGC ATGTATTGGA GGCTTTCCAG TTCTGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058662.2
CDS	138..1739
Translation	MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWDRKKFMGTELNGKTLGILGLGRIGREVATRMQSFGMKTVGYDPIISPEVAASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDSTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEAMGTLMHAWAGSPKGTIQVVTQGTSLKNAGTCLSPAVIVGLLREASKQADVNLVNAKLLVKEAGLNVTTSHNPGVPGEQGSGECLLTVALAGAPYQAVGLVQGTTPMLQMLNGAVFRPEVPLRRGQPLLVFRAQPSDPGMLPTMIGLLAEAGVQLLSYQTSMVSDGEPWHVMGLSSLLPSLETWKQHVLEAFQFCF

Target ORF information:

RefSeq Version	NM_016966.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus 3-phosphoglycerate dehydrogenase (Phgdh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016966.3

ORF Insert Sequence:

ATGGCCTTCG CAAATCTGCG CAAAGTACTC ATCAGTGACA GCCTGGACCC CTGCTGCCGG 
AAGATCCTGC AAGATGGAGG GCTGCAGGTG GTGGAGAAGC AGAACTTGAG CAAGGAGGAG 
CTGATAGCTG AACTCCAGGA CTGTGAAGGC CTCATTGTCC GGTCTGCTAC TAAGGTCACT 
GCTGATGTCA TCAATGCAGC AGAGAAGCTC CAGGTGGTGG GCAGGGCTGG CACAGGTGTG 
GACAATGTGG ATCTGGAGGC TGCCACGAGG AAGGGCATCC TAGTCATGAA CACCCCCAAC 
GGGAACAGCC TCAGTGCTGC GGAGCTCACC TGTGGGATGA TCATGTGCCT GGCCAGGCAG 
ATTCCCCAGG CAACAGCTTC GATGAAAGAT GGCAAATGGG ACCGGAAGAA GTTCATGGGG 
ACAGAACTGA ACGGGAAGAC ACTGGGAATT CTTGGCCTCG GCAGAATTGG AAGAGAGGTG 
GCCACCCGAA TGCAATCCTT TGGAATGAAG ACTGTAGGCT ATGACCCCAT CATCTCTCCT 
GAAGTCGCCG CCTCCTTTGG TGTTCAGCAG CTGCCGCTGG AGGAGATCTG GCCTCTCTGT 
GACTTTATAA CTGTCCATAC CCCACTCCTG CCCTCTACCA CAGGCTTGCT GAATGACAGC 
ACCTTTGCTC AGTGCAAGAA AGGTGTGCGA GTAGTGAACT GTGCTCGAGG AGGCATTGTG 
GATGAAGGTG CCCTGCTGCG TGCCCTGCAG TCTGGTCAGT GTGCTGGGGC TGCACTGGAT 
GTGTTTACAG AAGAGCCACC ACGGGACCGG GCCTTAGTGG ACCACGAGAA TGTCATCAGC 
TGTCCCCACC TGGGTGCCAG CACCAAGGAA GCCCAGAGCC GCTGTGGGGA GGAAATCGCA 
GTCCAGTTTG TGGACATGGT GAAGGGGAAA TCTCTAACAG GCGTTGTGAA CGCCCAGGCC 
CTCACCAGTG CCTTCTCTCC ACACACCAAG CCTTGGATTG GTCTGGCAGA AGCAATGGGC 
ACGCTGATGC ACGCCTGGGC TGGCTCCCCT AAAGGGACCA TCCAGGTGGT TACACAAGGA 
ACATCTCTGA AGAATGCTGG GACCTGCCTG AGCCCTGCAG TCATTGTCGG CCTTCTGAGA 
GAAGCATCTA AGCAGGCAGA CGTGAACTTG GTGAACGCTA AGCTACTGGT GAAAGAGGCT 
GGCCTCAATG TCACCACCTC CCACAACCCT GGGGTTCCAG GGGAGCAGGG TAGCGGGGAA 
TGCCTCCTGA CTGTGGCCCT GGCAGGTGCT CCCTACCAAG CCGTGGGCTT GGTCCAGGGC 
ACCACACCAA TGCTGCAGAT GCTCAACGGA GCTGTCTTCA GACCAGAGGT GCCACTACGC 
AGGGGCCAAC CCCTGCTCGT ATTCCGGGCT CAGCCCTCCG ACCCTGGGAT GCTGCCCACT 
ATGATTGGCC TCCTGGCAGA GGCGGGTGTA CAGCTGCTGT CCTACCAAAC CTCCATGGTG 
TCTGACGGAG AGCCCTGGCA CGTCATGGGC CTGTCCTCCC TGCTGCCCAG CCTGGAAACA 
TGGAAGCAGC ATGTATTGGA GGCTTTCCAG TTCTGCTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Steroid receptor coactivators 1 and 2 mediate fetal-to-maternal signaling that initiates parturition.
The Journal of clinical investigation125(7)2808-24(2015 Jul)
Gao L,Rabbitt EH,Condon JC,Renthal NE,Johnston JM,Mitsche MA,Chambon P,Xu J,O'Malley BW,Mendelson CR

Requirement of the expression of 3-phosphoglycerate dehydrogenase for traversing S phase in murine T lymphocytes following immobilized anti-CD3 activation.
Cellular immunology287(2)78-85(2014 Feb)
Jun do Y,Taub D,Chrest FJ,Kim YH

D-serine in glia and neurons derives from 3-phosphoglycerate dehydrogenase.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(30)12464-9(2013 Jul)
Ehmsen JT,Ma TM,Sason H,Rosenberg D,Ogo T,Furuya S,Snyder SH,Wolosker H

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Phgdh cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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