Lrrk1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrrk1
Entrez Gene ID	233328
Full Name	leucine-rich repeat kinase 1
Synonyms	AW319595,C230002E15Rik,D130026O16Rik,mKIAA1790
General protein information	Preferred Names leucine-rich repeat kinase 1 Names leucine-rich repeat serine/threonine-protein kinase 1 lrrk1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 C

mRNA and Protein(s)

mRNA	Protein	Name
XM_006540839.3	XP_006540902.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
NM_146191.3	NP_666303.3	leucine-rich repeat serine/threonine-protein kinase 1
XM_006540840.3	XP_006540903.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X3
XM_006540838.3	XP_006540901.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
XM_006540839.4	XP_006540902.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
XM_006540841.1	XP_006540904.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X4
XM_006540840.4	XP_006540903.1	leucine-rich repeat serine/threonine-protein kinase 1 isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	lrrk1	XP_002667476.3
Bos taurus (cattle)	LRRK1	NP_001192703.1
Xenopus tropicalis (tropical clawed frog)	lrrk1	XP_002939309.2
Homo sapiens (human)	LRRK1	NP_078928.3
Pan troglodytes (chimpanzee)	LRRK1	XP_510623.3
Rattus norvegicus (Norway rat)	Lrrk1	NP_001178553.1
	LRRK1	XP_001084079.1
Canis lupus familiaris (dog)	LRRK1	XP_005618338.1
Mus musculus (house mouse)	Lrrk1	NP_666303.3
Gallus gallus (chicken)	LRRK1	XP_003641870.2

Gene Ontology
Bibliography

Related articles in PubMed

Age-Dependent Dopaminergic Neurodegeneration and Impairment of the Autophagy-Lysosomal Pathway in LRRK-Deficient Mice.
Giaime E, Tong Y, Wagner LK, Yuan Y, Huang G, Shen J
Neuron96(4)796-807.e6(2017 Nov)

Leucine-rich repeat kinase-1 regulates osteoclast function by modulating RAC1/Cdc42 Small GTPase phosphorylation and activation.
Zeng C, Goodluck H, Qin X, Liu B, Mohan S, Xing W
American journal of physiology. Endocrinology and metabolism311(4)E772-E780(2016 Oct)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

LRRK1 is critical in the regulation of B-cell responses and CARMA1-dependent NF-?B activation.
Morimoto K, Baba Y, Shinohara H, Kang S, Nojima S, Kimura T, Ito D, Yoshida Y, Maeda Y, Sarashina-Kida H, Nishide M, Hosokawa T, Kato Y, Hayama Y, Kinehara Y, Okuno T, Takamatsu H, Hirano T, Shima Y, Narazaki M, Kurosaki T, Toyofuku T, Kumanogoh A
Scientific reports625738(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings revealed an essential role of LRRK in the survival of DA neurons and in the regulation of the autophagy-lysosomal pathway in the aging brain.
Title: Age-Dependent Dopaminergic Neurodegeneration and Impairment of the Autophagy-Lysosomal Pathway in LRRK-Deficient Mice.

Lrrk1 deficiency in osteoclasts resulted in reduced phosphorylation and activation of RAC1/Cdc42
Title: Leucine-rich repeat kinase-1 regulates osteoclast function by modulating RAC1/Cdc42 Small GTPase phosphorylation and activation.

the spatiotemporal regulation of Rab7 activity during tunicamycin-induced autophagy is regulated by LRRK1.
Title: Leucine-Rich Repeat Kinase 1 Regulates Autophagy through Turning On TBC1D2-Dependent Rab7 Inactivation.

FIH-1 has a role in disrupting the LRRK1/EGFR complex to positively regulate keratinocyte migration
Title: FIH-1 disrupts an LRRK1/EGFR complex to positively regulate keratinocyte migration.

Skeletal phenotypes in Lrrk1 and Lrrk2 knockout (KO) mice, were investigated.
Title: Targeted disruption of leucine-rich repeat kinase 1 but not leucine-rich repeat kinase 2 in mice causes severe osteopetrosis.

The following Lrrk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrrk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu38812	XM_006540840.4 Latest version!	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu38813	XM_006540841.1 Latest version!	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu38811	XM_006540839.4 Latest version!	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu17582	NM_146191.3 Latest version!	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu38810	XM_006540838.3 Latest version!	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OMu38811	XM_006540839.3	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu38812	XM_006540840.3	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrrk1 ( XM_006540840.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540903.1 Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X4, mRNA.

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CloneID	OMu38812
Clone ID Related Accession (Same CDS sequence)	XM_006540840.3 , XM_006540840.4
Accession Version	XM_006540840.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540840.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAAT TCCCAGCTTT CCTAAGTGAG TCTTTGGAGG TCCTTTGTCT GAATGACAAC 
CATCTTGATG CTGTTCCCCC ATCGGTCTGC CTGCTGAAGA ACCTCTCAGA GCTCTACTTG 
GGCAATAACC CTGGCCTCCG AGAGCTCCCT CCGGAGCTAG GCCAGCTGGG GAACCTCTGG 
CAACTGGACA TTGAAGATCT GAACATTAGC AACGTGCCTG CGGAGGTCAG GAAAGAAGGC 
CCGAAAGCAA CACTGTCTTT CCTGCGTGCT CAGCTGCGCA AAGCAGAGAA GTGTAAGCTG 
ATGAAGATGA TCCTCGTGGG TCCCCCGCGC CAGGGCAAGT CCACACTCCT GGAGATCTTA 
CAGACCGGGA AGGCCCCGCA GCTAGCACAC AGTGAAGCCA CCATCAGGAC CACCAAGTGG 
GAGCTCCAGA GACCAGCAGG CTCCAAAGCC AAGGTTGAGT CCGTAGAGTT CAACGTCTGG 
GACATCGGGG GTCCGGCCAG CATGGCCACG GTCAATCAGT GCTTCTTCAC AGACAAGGCG 
CTGTATGTGG TGGTCTGGAA CCTGGCACTG GGAGAGGAAG CCGTGGCCAA CCTCCAGTTC 
TGGCTGCTCA ACATTGAGGC CAAGGCCCCA AATGCCGTTG TGCTGGTGGT CGGGACACAC 
CTGGACCTAA TCGAAGCTAA GTTCCGAGTG GAGAGGATAG CAACCCTGCG TGCCTACGTG 
CTGGCCCTCT GCCGCTCGCC ATCGGGCTCC AGGGCTACGG GCTTCCCGGA CATCACTTTC 
AAACACTTGC ATGAGATCTC CTGCAAGAAT CTGGAAGGGC AGGAGGGGCT GCGGCAACTG 
ATCTTCCACG TCACGTGCAA CATGAAGGAT GTGGGTAGCA CTATCGGCTG TCAAAAACTC 
GCCGGCAGGC TGATCCCCAG GAGCTACATA AGCCTACAGG AGGCCGTGCT GGCTGAGCAA 
CAGCGCCGCA GTCTGGGTGA CCAAGTACAG TATCTGACGG ACAGGCAGCT GGACCAGCTG 
GTAGAGCAGA CGCCTGGCAA TGATATCAAA GACTATGAAG ACCTGCAGTC TGCCATCAGC 
TTCCTCATAG AAACTGGGAC CCTGCTGCAC TTTCCAGACA CGAGCCATGG CCTAAGGAAC 
CTCTACTTCC TGGATCCCAT CTGGCTCTCT GAATGCCTGC AGAGGATCTT TAACATCAAG 
GGCTCGCGGT CAGTGGCAAA GAATGGGGTG ATCCAAGCGG AAGACCTCAG GATGCTGCTG 
GTGGGGACAG GCTTCACACA GCAGACTGAG GAGCAATACT TCCAGTTCCT TGCCAAGTTC 
GAGATTGCCC TCCCTGTTGC CAATGACAGC TACCTCCTAC CACACCTCCT TCCATCCAAG 
CCTGGGCTGG ACACCCACAG CATGCGGCAC CCAATGGCTA ACACCATCCA GCGGGTGTTT 
AAGATGAGCT TCGTGCCTGT TGGCTTCTGG CAACGGTTCA TAGCACGGAT GCTAATCAGC 
TTGGCTGAGA TGGACCTGCA GCTTTTTGAA AACAAGAAGA ATACGAAAAG CAGGAACCGG 
AAAGTCACTA TTTACAGTTT TACAGGGAGC CAGAGAAACC GCTGCAGTAC ATTCCGAGTC 
CGAAGAAATC AGACCATCTA CTGGCAGGAA GGGCTGCTGG TCACTTTCGA TGGGGGCTAC 
CTCAGTGTGG AATCCTCAGA TGTGAACTGG AAAAAGAAAA AAAGCGGAGG AATTAAAATC 
ATCTGCCAGT CAGAGATGAG GGACTTCTCG GCAATGGCTT TTATCACAGA CCATGTCAAC 
TCCCTGATTG ACCAGTGGTT CCCTGCCCTG ACGGCCACAG AAAGTGACGG GACCCCACTG 
ATGGAGCAGT ACGTACCCTG CCCTGTCTGT GAAGCATCCT GGGCCCAGCA TGCAGATCCA 
AACGAGAGGT CAGAGAGCGT GCAGTACTTT GATATGGAAG ACTGTGTGCT CACAGCCATC 
GAACGGGACT TCATCTCCTG CCCCAGACAC CCAGACCTCC CAGTACCACT TCAGGAGCTT 
GTACCAGAGC TGTTCATGAC TGACTTCCCA GCCAGGCTCT TCCTGGAGAA CAGCAAGCTG 
GAGCACACGG AGGGTGAGAA CAGCATTCTG GGCCAAGGTG GCAGTGGCAC AGTCATCTAC 
CAGGCCCGGT ACCAGGGCCA GCCTGTGGCT GTGAAGAGGT TCCATATCAA GAAGTTCAAG 
AATTCTGCTA ATGCCCCAGC AGACACCATG CTGCGGCACC TGAGAGCCAT GGATGCCATG 
AAGAACTTCT CAGATTTCCG TCAGGAGGCC AGCATGCTGC ATGCCTTGCA GCATCCTTGC 
ATCGTGTCAC TCATCGGCAT CAGCATCCAC CCGCTCTGCT TCGCCTTGGA GCTGGCCCCG 
CTGGGCAGTC TCAACACCGT GCTATCTGAG AATGCCAAAG ATTCTTCCTT TATGCCCCTG 
GGACACATGC TCACCCAAAA GATAGCCTAC CAGATTGCCT CGGGCCTGGC CTACCTGCAC 
AAGAAAAACA TCATCTTCTG TGACCTCAAG TCGGACAACA TCCTAGTGTG GTCGCTGAGC 
GCCAAGGAGC ATATCAACAT CAAACTGTCA GACTACGGCA TCTCCAGGCA GTCCTTTCAC 
GAGGGGGCCC TGGGTGTGGA GGGCACCCCA GGTTACCAGG CCCCAGAGAT CAGGCCTCGC 
ATCGTGTATG ACGAGAAGGT AGATATGTTC TCCTACGGCA TGGTGCTATA CGAGCTGCTG 
TCAGGACAGC GTCCTGCACT GGGCCACCAC CAACTTCAGA TTGTCAAGAA GTTGTCCAAG 
GGCATCCGCC CAGTTCTGGG GCAGCCGGAG GAAGTTCAGT TCCATCGGCT GCAGGCGCTC 
ATGATGGAGT GCTGGGACAC AAAGCCGGAG AAGCGCCCCC TGGCCTTGTC CGTGGTGAGC 
CAGATGAAAG ACCCAACCTT TGCCACCTTC ATGTACATGC TGCCCTGTGG GAAGCAGTCG 
GCCTTCTTCT CATCCCAGAG CCAGGAGTAC ACTGTGGTGT TCTGGGATGG GAAGGAGGAG 
TCCAGGAATT ACACGGTGGT CAACACAGAG AAGGGCCTTC TGGAAGTACA GAGGATGACC 
TGCCCCGGGA TGAAGTTGAG CTGTCAGCTC AAGGTCCAGA GCTCAGTGTG GATAGCCACG 
GAGGACCAGA AAATCTATAT CTACAGCCTT AAGGGTATGT GCCCATTAAG CGTGCCCCAG 
CAGGCCCTGG ACACCCCAGC TGTCGTCACC TGTTTCTTGG CAGTACCTGT TATCAAAAAG 
AACTCCTTCC TGGTGCTGGC AGGCCTGGCT GACGGACTCG TGGCTGTGTT TCCTGTGGCA 
CGGGGCACCC CAAAAGAAAG CTGCTCCTAC CTGTGCTCCC ACACAGCCAA CAGGTCCAAG 
TTCTGCATCC CCGACGAAGA TGCACGGCAG AACCCTTACC CAGTGAAGGC AATGGAAGTG 
GTCAACAGTG GGTCTGAGGT CTGGTACAGC AACGGGCCTG GCCTCCTCGT CATTGACTGC 
ACCATCCTGG ACATCAGCAG GCGGCTGGAG CCCTACGCAG CTCCGTCCAT GGTCACGTCA 
CTTGTGTGCA GCTCAGACTG CAGAGGAGAG GAGATGGTCT GGTGCCTCGA CGACAAGGCC 
AATTGCTTAG TGATGTACCA CTCAGCCACC TACCAGCTCT GTGCCCGGTA CTTCTGTGGG 
GACCCCAATC CTCTCAGGGA CACGTTTTCT GTGCAGCCTT CAGTCCTAGA AACCCCAGGC 
AGTCACAAAA CCACCTCAAA GGGGCCTGTG GAAGAGTGCA TCGCGGATGT GAGCATCATG 
TACAGCGAGG AGCTGGGCAC GCAGATCCTC ACCCACCAGG ACTCGCTCAC CGACTACTGC 
TCCATGTCCT CTTATTCTTC GTCCCCACCC CACCAAGATC CTAGGTCCCC TTCCAGTCTG 
CCCAGCTCCC TCACCAGCTA TTCCAGTGTG CCTTTCTCTG CCAACTATGA AGACTCAGAC 
AGGCTGCAGG AGCCCAGTGT CACCTCTGAC AGAACTGAGC ATGACCTGAG CCCCATGGAT 
GGGGAGACGT TCAGCCAGCA CCTGCAGGCT GTGAAGGTGC TTGCTGTCAA AGACCTCATT 
TGGGTTCCTA GACATGGAGG AGATATCATT GTCATCGGCC TGGAGAAGGA TTCAGGTGCC 
CAGCGGGGCA GAGTCATCGC TGTTTTAAAA GCCCGAGAGC TCAATCGGCA CGGGGTTCTG 
GTGGATGCCG CAGTGGTGGC AAAGGACACC GTAGTGTGCA GCTTTGCAAA TGAAAACACA 
GAGTGGTGCC TGGCTGTCTG GAGGGGCTGG GGCGCCAGGG AGTTTGACAT CTTCTACCAG 
TCCTATGAGG AGCTGGGCCG GCTGGAGGCT TGTACTCGCA AGAGAAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540903.1
CDS	530..4960
Translation	MLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKNLSELYLGNNPGLRELPPELGQLGNLWQLDIEDLNISNVPAEVRKEGPKATLSFLRAQLRKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQLAHSEATIRTTKWELQRPAGSKAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAGRLIPRSYISLQEAVLAEQQRRSLGDQVQYLTDRQLDQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIQAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQARYQGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLSAKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIVKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSAFFSSQSQEYTVVFWDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWIATEDQKIYIYSLKGMCPLSVPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFPVARGTPKESCSYLCSHTANRSKFCIPDEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCTILDISRRLEPYAAPSMVTSLVCSSDCRGEEMVWCLDDKANCLVMYHSATYQLCARYFCGDPNPLRDTFSVQPSVLETPGSHKTTSKGPVEECIADVSIMYSEELGTQILTHQDSLTDYCSMSSYSSSPPHQDPRSPSSLPSSLTSYSSVPFSANYEDSDRLQEPSVTSDRTEHDLSPMDGETFSQHLQAVKVLAVKDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNRHGVLVDAAVVAKDTVVCSFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR

Target ORF information:

RefSeq Version	XM_006540840.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540840.4

ORF Insert Sequence:

ATGTTGGAAT TCCCAGCTTT CCTAAGTGAG TCTTTGGAGG TCCTTTGTCT GAATGACAAC 
CATCTTGATG CTGTTCCCCC ATCGGTCTGC CTGCTGAAGA ACCTCTCAGA GCTCTACTTG 
GGCAATAACC CTGGCCTCCG AGAGCTCCCT CCGGAGCTAG GCCAGCTGGG GAACCTCTGG 
CAACTGGACA TTGAAGATCT GAACATTAGC AACGTGCCTG CGGAGGTCAG GAAAGAAGGC 
CCGAAAGCAA CACTGTCTTT CCTGCGTGCT CAGCTGCGCA AAGCAGAGAA GTGTAAGCTG 
ATGAAGATGA TCCTCGTGGG TCCCCCGCGC CAGGGCAAGT CCACACTCCT GGAGATCTTA 
CAGACCGGGA AGGCCCCGCA GCTAGCACAC AGTGAAGCCA CCATCAGGAC CACCAAGTGG 
GAGCTCCAGA GACCAGCAGG CTCCAAAGCC AAGGTTGAGT CCGTAGAGTT CAACGTCTGG 
GACATCGGGG GTCCGGCCAG CATGGCCACG GTCAATCAGT GCTTCTTCAC AGACAAGGCG 
CTGTATGTGG TGGTCTGGAA CCTGGCACTG GGAGAGGAAG CCGTGGCCAA CCTCCAGTTC 
TGGCTGCTCA ACATTGAGGC CAAGGCCCCA AATGCCGTTG TGCTGGTGGT CGGGACACAC 
CTGGACCTAA TCGAAGCTAA GTTCCGAGTG GAGAGGATAG CAACCCTGCG TGCCTACGTG 
CTGGCCCTCT GCCGCTCGCC ATCGGGCTCC AGGGCTACGG GCTTCCCGGA CATCACTTTC 
AAACACTTGC ATGAGATCTC CTGCAAGAAT CTGGAAGGGC AGGAGGGGCT GCGGCAACTG 
ATCTTCCACG TCACGTGCAA CATGAAGGAT GTGGGTAGCA CTATCGGCTG TCAAAAACTC 
GCCGGCAGGC TGATCCCCAG GAGCTACATA AGCCTACAGG AGGCCGTGCT GGCTGAGCAA 
CAGCGCCGCA GTCTGGGTGA CCAAGTACAG TATCTGACGG ACAGGCAGCT GGACCAGCTG 
GTAGAGCAGA CGCCTGGCAA TGATATCAAA GACTATGAAG ACCTGCAGTC TGCCATCAGC 
TTCCTCATAG AAACTGGGAC CCTGCTGCAC TTTCCAGACA CGAGCCATGG CCTAAGGAAC 
CTCTACTTCC TGGATCCCAT CTGGCTCTCT GAATGCCTGC AGAGGATCTT TAACATCAAG 
GGCTCGCGGT CAGTGGCAAA GAATGGGGTG ATCCAAGCGG AAGACCTCAG GATGCTGCTG 
GTGGGGACAG GCTTCACACA GCAGACTGAG GAGCAATACT TCCAGTTCCT TGCCAAGTTC 
GAGATTGCCC TCCCTGTTGC CAATGACAGC TACCTCCTAC CACACCTCCT TCCATCCAAG 
CCTGGGCTGG ACACCCACAG CATGCGGCAC CCAATGGCTA ACACCATCCA GCGGGTGTTT 
AAGATGAGCT TCGTGCCTGT TGGCTTCTGG CAACGGTTCA TAGCACGGAT GCTAATCAGC 
TTGGCTGAGA TGGACCTGCA GCTTTTTGAA AACAAGAAGA ATACGAAAAG CAGGAACCGG 
AAAGTCACTA TTTACAGTTT TACAGGGAGC CAGAGAAACC GCTGCAGTAC ATTCCGAGTC 
CGAAGAAATC AGACCATCTA CTGGCAGGAA GGGCTGCTGG TCACTTTCGA TGGGGGCTAC 
CTCAGTGTGG AATCCTCAGA TGTGAACTGG AAAAAGAAAA AAAGCGGAGG AATTAAAATC 
ATCTGCCAGT CAGAGATGAG GGACTTCTCG GCAATGGCTT TTATCACAGA CCATGTCAAC 
TCCCTGATTG ACCAGTGGTT CCCTGCCCTG ACGGCCACAG AAAGTGACGG GACCCCACTG 
ATGGAGCAGT ACGTACCCTG CCCTGTCTGT GAAGCATCCT GGGCCCAGCA TGCAGATCCA 
AACGAGAGGT CAGAGAGCGT GCAGTACTTT GATATGGAAG ACTGTGTGCT CACAGCCATC 
GAACGGGACT TCATCTCCTG CCCCAGACAC CCAGACCTCC CAGTACCACT TCAGGAGCTT 
GTACCAGAGC TGTTCATGAC TGACTTCCCA GCCAGGCTCT TCCTGGAGAA CAGCAAGCTG 
GAGCACACGG AGGGTGAGAA CAGCATTCTG GGCCAAGGTG GCAGTGGCAC AGTCATCTAC 
CAGGCCCGGT ACCAGGGCCA GCCTGTGGCT GTGAAGAGGT TCCATATCAA GAAGTTCAAG 
AATTCTGCTA ATGCCCCAGC AGACACCATG CTGCGGCACC TGAGAGCCAT GGATGCCATG 
AAGAACTTCT CAGATTTCCG TCAGGAGGCC AGCATGCTGC ATGCCTTGCA GCATCCTTGC 
ATCGTGTCAC TCATCGGCAT CAGCATCCAC CCGCTCTGCT TCGCCTTGGA GCTGGCCCCG 
CTGGGCAGTC TCAACACCGT GCTATCTGAG AATGCCAAAG ATTCTTCCTT TATGCCCCTG 
GGACACATGC TCACCCAAAA GATAGCCTAC CAGATTGCCT CGGGCCTGGC CTACCTGCAC 
AAGAAAAACA TCATCTTCTG TGACCTCAAG TCGGACAACA TCCTAGTGTG GTCGCTGAGC 
GCCAAGGAGC ATATCAACAT CAAACTGTCA GACTACGGCA TCTCCAGGCA GTCCTTTCAC 
GAGGGGGCCC TGGGTGTGGA GGGCACCCCA GGTTACCAGG CCCCAGAGAT CAGGCCTCGC 
ATCGTGTATG ACGAGAAGGT AGATATGTTC TCCTACGGCA TGGTGCTATA CGAGCTGCTG 
TCAGGACAGC GTCCTGCACT GGGCCACCAC CAACTTCAGA TTGTCAAGAA GTTGTCCAAG 
GGCATCCGCC CAGTTCTGGG GCAGCCGGAG GAAGTTCAGT TCCATCGGCT GCAGGCGCTC 
ATGATGGAGT GCTGGGACAC AAAGCCGGAG AAGCGCCCCC TGGCCTTGTC CGTGGTGAGC 
CAGATGAAAG ACCCAACCTT TGCCACCTTC ATGTACATGC TGCCCTGTGG GAAGCAGTCG 
GCCTTCTTCT CATCCCAGAG CCAGGAGTAC ACTGTGGTGT TCTGGGATGG GAAGGAGGAG 
TCCAGGAATT ACACGGTGGT CAACACAGAG AAGGGCCTTC TGGAAGTACA GAGGATGACC 
TGCCCCGGGA TGAAGTTGAG CTGTCAGCTC AAGGTCCAGA GCTCAGTGTG GATAGCCACG 
GAGGACCAGA AAATCTATAT CTACAGCCTT AAGGGTATGT GCCCATTAAG CGTGCCCCAG 
CAGGCCCTGG ACACCCCAGC TGTCGTCACC TGTTTCTTGG CAGTACCTGT TATCAAAAAG 
AACTCCTTCC TGGTGCTGGC AGGCCTGGCT GACGGACTCG TGGCTGTGTT TCCTGTGGCA 
CGGGGCACCC CAAAAGAAAG CTGCTCCTAC CTGTGCTCCC ACACAGCCAA CAGGTCCAAG 
TTCTGCATCC CCGACGAAGA TGCACGGCAG AACCCTTACC CAGTGAAGGC AATGGAAGTG 
GTCAACAGTG GGTCTGAGGT CTGGTACAGC AACGGGCCTG GCCTCCTCGT CATTGACTGC 
ACCATCCTGG ACATCAGCAG GCGGCTGGAG CCCTACGCAG CTCCGTCCAT GGTCACGTCA 
CTTGTGTGCA GCTCAGACTG CAGAGGAGAG GAGATGGTCT GGTGCCTCGA CGACAAGGCC 
AATTGCTTAG TGATGTACCA CTCAGCCACC TACCAGCTCT GTGCCCGGTA CTTCTGTGGG 
GACCCCAATC CTCTCAGGGA CACGTTTTCT GTGCAGCCTT CAGTCCTAGA AACCCCAGGC 
AGTCACAAAA CCACCTCAAA GGGGCCTGTG GAAGAGTGCA TCGCGGATGT GAGCATCATG 
TACAGCGAGG AGCTGGGCAC GCAGATCCTC ACCCACCAGG ACTCGCTCAC CGACTACTGC 
TCCATGTCCT CTTATTCTTC GTCCCCACCC CACCAAGATC CTAGGTCCCC TTCCAGTCTG 
CCCAGCTCCC TCACCAGCTA TTCCAGTGTG CCTTTCTCTG CCAACTATGA AGACTCAGAC 
AGGCTGCAGG AGCCCAGTGT CACCTCTGAC AGAACTGAGC ATGACCTGAG CCCCATGGAT 
GGGGAGACGT TCAGCCAGCA CCTGCAGGCT GTGAAGGTGC TTGCTGTCAA AGACCTCATT 
TGGGTTCCTA GACATGGAGG AGATATCATT GTCATCGGCC TGGAGAAGGA TTCAGGTGCC 
CAGCGGGGCA GAGTCATCGC TGTTTTAAAA GCCCGAGAGC TCAATCGGCA CGGGGTTCTG 
GTGGATGCCG CAGTGGTGGC AAAGGACACC GTAGTGTGCA GCTTTGCAAA TGAAAACACA 
GAGTGGTGCC TGGCTGTCTG GAGGGGCTGG GGCGCCAGGG AGTTTGACAT CTTCTACCAG 
TCCTATGAGG AGCTGGGCCG GCTGGAGGCT TGTACTCGCA AGAGAAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrk1 ( XM_006540841.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540904.1 Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X5, mRNA.

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CloneID	OMu38813
Clone ID Related Accession (Same CDS sequence)	XM_006540841.1
Accession Version	XM_006540841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGG TCAATCAGTG CTTCTTCACA GACAAGGCGC TGTATGTGGT GGTCTGGAAC 
CTGGCACTGG GAGAGGAAGC CGTGGCCAAC CTCCAGTTCT GGCTGCTCAA CATTGAGGCC 
AAGGCCCCAA ATGCCGTTGT GCTGGTGGTC GGGACACACC TGGACCTAAT CGAAGCTAAG 
TTCCGAGTGG AGAGGATAGC AACCCTGCGT GCCTACGTGC TGGCCCTCTG CCGCTCGCCA 
TCGGGCTCCA GGGCTACGGG CTTCCCGGAC ATCACTTTCA AACACTTGCA TGAGATCTCC 
TGCAAGAATC TGGAAGGGCA GGAGGGGCTG CGGCAACTGA TCTTCCACGT CACGTGCAAC 
ATGAAGGATG TGGGTAGCAC TATCGGCTGT CAAAAACTCG CCGGCAGGCT GATCCCCAGG 
AGCTACATAA GCCTACAGGA GGCCGTGCTG GCTGAGCAAC AGCGCCGCAG TCTGGGTGAC 
CAAGTACAGT ATCTGACGGA CAGGCAGCTG GACCAGCTGG TAGAGCAGAC GCCTGGCAAT 
GATATCAAAG ACTATGAAGA CCTGCAGTCT GCCATCAGCT TCCTCATAGA AACTGGGACC 
CTGCTGCACT TTCCAGACAC GAGCCATGGC CTAAGGAACC TCTACTTCCT GGATCCCATC 
TGGCTCTCTG AATGCCTGCA GAGGATCTTT AACATCAAGG GCTCGCGGTC AGTGGCAAAG 
AATGGGGTGA TCCAAGCGGA AGACCTCAGG ATGCTGCTGG TGGGGACAGG CTTCACACAG 
CAGACTGAGG AGCAATACTT CCAGTTCCTT GCCAAGTTCG AGATTGCCCT CCCTGTTGCC 
AATGACAGCT ACCTCCTACC ACACCTCCTT CCATCCAAGC CTGGGCTGGA CACCCACAGC 
ATGCGGCACC CAATGGCTAA CACCATCCAG CGGGTGTTTA AGATGAGCTT CGTGCCTGTT 
GGCTTCTGGC AACGGTTCAT AGCACGGATG CTAATCAGCT TGGCTGAGAT GGACCTGCAG 
CTTTTTGAAA ACAAGAAGAA TACGAAAAGC AGGAACCGGA AAGTCACTAT TTACAGTTTT 
ACAGGGAGCC AGAGAAACCG CTGCAGTACA TTCCGAGTCC GAAGAAATCA GACCATCTAC 
TGGCAGGAAG GGCTGCTGGT CACTTTCGAT GGGGGCTACC TCAGTGTGGA ATCCTCAGAT 
GTGAACTGGA AAAAGAAAAA AAGCGGAGGA ATTAAAATCA TCTGCCAGTC AGAGATGAGG 
GACTTCTCGG CAATGGCTTT TATCACAGAC CATGTCAACT CCCTGATTGA CCAGTGGTTC 
CCTGCCCTGA CGGCCACAGA AAGTGACGGG ACCCCACTGA TGGAGCAGTA CGTACCCTGC 
CCTGTCTGTG AAGCATCCTG GGCCCAGCAT GCAGATCCAA ACGAGAGGTC AGAGAGCGTG 
CAGTACTTTG ATATGGAAGA CTGTGTGCTC ACAGCCATCG AACGGGACTT CATCTCCTGC 
CCCAGACACC CAGACCTCCC AGTACCACTT CAGGAGCTTG TACCAGAGCT GTTCATGACT 
GACTTCCCAG CCAGGCTCTT CCTGGAGAAC AGCAAGCTGG AGCACACGGA GGGTGAGAAC 
AGCATTCTGG GCCAAGGTGG CAGTGGCACA GTCATCTACC AGGCCCGGTA CCAGGGCCAG 
CCTGTGGCTG TGAAGAGGTT CCATATCAAG AAGTTCAAGA ATTCTGCTAA TGCCCCAGCA 
GACACCATGC TGCGGCACCT GAGAGCCATG GATGCCATGA AGAACTTCTC AGATTTCCGT 
CAGGAGGCCA GCATGCTGCA TGCCTTGCAG CATCCTTGCA TCGTGTCACT CATCGGCATC 
AGCATCCACC CGCTCTGCTT CGCCTTGGAG CTGGCCCCGC TGGGCAGTCT CAACACCGTG 
CTATCTGAGA ATGCCAAAGA TTCTTCCTTT ATGCCCCTGG GACACATGCT CACCCAAAAG 
ATAGCCTACC AGATTGCCTC GGGCCTGGCC TACCTGCACA AGAAAAACAT CATCTTCTGT 
GACCTCAAGT CGGACAACAT CCTAGTGTGG TCGCTGAGCG CCAAGGAGCA TATCAACATC 
AAACTGTCAG ACTACGGCAT CTCCAGGCAG TCCTTTCACG AGGGGGCCCT GGGTGTGGAG 
GGCACCCCAG GTTACCAGGC CCCAGAGATC AGGCCTCGCA TCGTGTATGA CGAGAAGGTA 
GATATGTTCT CCTACGGCAT GGTGCTATAC GAGCTGCTGT CAGGACAGCG TCCTGCACTG 
GGCCACCACC AACTTCAGAT TGTCAAGAAG TTGTCCAAGG GCATCCGCCC AGTTCTGGGG 
CAGCCGGAGG AAGTTCAGTT CCATCGGCTG CAGGCGCTCA TGATGGAGTG CTGGGACACA 
AAGCCGGAGA AGCGCCCCCT GGCCTTGTCC GTGGTGAGCC AGATGAAAGA CCCAACCTTT 
GCCACCTTCA TGTACATGCT GCCCTGTGGG AAGCAGTCGG CCTTCTTCTC ATCCCAGAGC 
CAGGAGTACA CTGTGGTGTT CTGGGATGGG AAGGAGGAGT CCAGGAATTA CACGGTGGTC 
AACACAGAGA AGGGCCTTCT GGAAGTACAG AGGATGACCT GCCCCGGGAT GAAGTTGAGC 
TGTCAGCTCA AGGTCCAGAG CTCAGTGTGG ATAGCCACGG AGGACCAGAA AATCTATATC 
TACAGCCTTA AGGGTATGTG CCCATTAAGC GTGCCCCAGC AGGCCCTGGA CACCCCAGCT 
GTCGTCACCT GTTTCTTGGC AGTACCTGTT ATCAAAAAGA ACTCCTTCCT GGTGCTGGCA 
GGCCTGGCTG ACGGACTCGT GGCTGTGTTT CCTGTGGCAC GGGGCACCCC AAAAGAAAGC 
TGCTCCTACC TGTGCTCCCA CACAGCCAAC AGGTCCAAGT TCTGCATCCC CGACGAAGAT 
GCACGGCAGA ACCCTTACCC AGTGAAGGCA ATGGAAGTGG TCAACAGTGG GTCTGAGGTC 
TGGTACAGCA ACGGGCCTGG CCTCCTCGTC ATTGACTGCA CCATCCTGGA CATCAGCAGG 
CGGCTGGAGC CCTACGCAGC TCCGTCCATG GTCACGTCAC TTGTGTGCAG CTCAGACTGC 
AGAGGAGAGG AGATGGTCTG GTGCCTCGAC GACAAGGCCA ATTGCTTAGT GATGTACCAC 
TCAGCCACCT ACCAGCTCTG TGCCCGGTAC TTCTGTGGGG ACCCCAATCC TCTCAGGGAC 
ACGTTTTCTG TGCAGCCTTC AGTCCTAGAA ACCCCAGGCA GTCACAAAAC CACCTCAAAG 
GGGCCTGTGG AAGAGTGCAT CGCGGATGTG AGCATCATGT ACAGCGAGGA GCTGGGCACG 
CAGATCCTCA CCCACCAGGA CTCGCTCACC GACTACTGCT CCATGTCCTC TTATTCTTCG 
TCCCCACCCC ACCAAGATCC TAGGTCCCCT TCCAGTCTGC CCAGCTCCCT CACCAGCTAT 
TCCAGTGTGC CTTTCTCTGC CAACTATGAA GACTCAGACA GGCTGCAGGA GCCCAGTGTC 
ACCTCTGACA GAACTGAGCA TGACCTGAGC CCCATGGATG GGGAGACGTT CAGCCAGCAC 
CTGCAGGCTG TGAAGGTGCT TGCTGTCAAA GACCTCATTT GGGTTCCTAG ACATGGAGGA 
GATATCATTG TCATCGGCCT GGAGAAGGAT TCAGGTGCCC AGCGGGGCAG AGTCATCGCT 
GTTTTAAAAG CCCGAGAGCT CAATCGGCAC GGGGTTCTGG TGGATGCCGC AGTGGTGGCA 
AAGGACACCG TAGTGTGCAG CTTTGCAAAT GAAAACACAG AGTGGTGCCT GGCTGTCTGG 
AGGGGCTGGG GCGCCAGGGA GTTTGACATC TTCTACCAGT CCTATGAGGA GCTGGGCCGG 
CTGGAGGCTT GTACTCGCAA GAGAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540904.1
CDS	142..4071
Translation	MATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAGRLIPRSYISLQEAVLAEQQRRSLGDQVQYLTDRQLDQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIQAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQARYQGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLSAKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIVKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSAFFSSQSQEYTVVFWDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWIATEDQKIYIYSLKGMCPLSVPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFPVARGTPKESCSYLCSHTANRSKFCIPDEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCTILDISRRLEPYAAPSMVTSLVCSSDCRGEEMVWCLDDKANCLVMYHSATYQLCARYFCGDPNPLRDTFSVQPSVLETPGSHKTTSKGPVEECIADVSIMYSEELGTQILTHQDSLTDYCSMSSYSSSPPHQDPRSPSSLPSSLTSYSSVPFSANYEDSDRLQEPSVTSDRTEHDLSPMDGETFSQHLQAVKVLAVKDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNRHGVLVDAAVVAKDTVVCSFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR

Target ORF information:

RefSeq Version	XM_006540841.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540841.1

ORF Insert Sequence:

ATGGCCACGG TCAATCAGTG CTTCTTCACA GACAAGGCGC TGTATGTGGT GGTCTGGAAC 
CTGGCACTGG GAGAGGAAGC CGTGGCCAAC CTCCAGTTCT GGCTGCTCAA CATTGAGGCC 
AAGGCCCCAA ATGCCGTTGT GCTGGTGGTC GGGACACACC TGGACCTAAT CGAAGCTAAG 
TTCCGAGTGG AGAGGATAGC AACCCTGCGT GCCTACGTGC TGGCCCTCTG CCGCTCGCCA 
TCGGGCTCCA GGGCTACGGG CTTCCCGGAC ATCACTTTCA AACACTTGCA TGAGATCTCC 
TGCAAGAATC TGGAAGGGCA GGAGGGGCTG CGGCAACTGA TCTTCCACGT CACGTGCAAC 
ATGAAGGATG TGGGTAGCAC TATCGGCTGT CAAAAACTCG CCGGCAGGCT GATCCCCAGG 
AGCTACATAA GCCTACAGGA GGCCGTGCTG GCTGAGCAAC AGCGCCGCAG TCTGGGTGAC 
CAAGTACAGT ATCTGACGGA CAGGCAGCTG GACCAGCTGG TAGAGCAGAC GCCTGGCAAT 
GATATCAAAG ACTATGAAGA CCTGCAGTCT GCCATCAGCT TCCTCATAGA AACTGGGACC 
CTGCTGCACT TTCCAGACAC GAGCCATGGC CTAAGGAACC TCTACTTCCT GGATCCCATC 
TGGCTCTCTG AATGCCTGCA GAGGATCTTT AACATCAAGG GCTCGCGGTC AGTGGCAAAG 
AATGGGGTGA TCCAAGCGGA AGACCTCAGG ATGCTGCTGG TGGGGACAGG CTTCACACAG 
CAGACTGAGG AGCAATACTT CCAGTTCCTT GCCAAGTTCG AGATTGCCCT CCCTGTTGCC 
AATGACAGCT ACCTCCTACC ACACCTCCTT CCATCCAAGC CTGGGCTGGA CACCCACAGC 
ATGCGGCACC CAATGGCTAA CACCATCCAG CGGGTGTTTA AGATGAGCTT CGTGCCTGTT 
GGCTTCTGGC AACGGTTCAT AGCACGGATG CTAATCAGCT TGGCTGAGAT GGACCTGCAG 
CTTTTTGAAA ACAAGAAGAA TACGAAAAGC AGGAACCGGA AAGTCACTAT TTACAGTTTT 
ACAGGGAGCC AGAGAAACCG CTGCAGTACA TTCCGAGTCC GAAGAAATCA GACCATCTAC 
TGGCAGGAAG GGCTGCTGGT CACTTTCGAT GGGGGCTACC TCAGTGTGGA ATCCTCAGAT 
GTGAACTGGA AAAAGAAAAA AAGCGGAGGA ATTAAAATCA TCTGCCAGTC AGAGATGAGG 
GACTTCTCGG CAATGGCTTT TATCACAGAC CATGTCAACT CCCTGATTGA CCAGTGGTTC 
CCTGCCCTGA CGGCCACAGA AAGTGACGGG ACCCCACTGA TGGAGCAGTA CGTACCCTGC 
CCTGTCTGTG AAGCATCCTG GGCCCAGCAT GCAGATCCAA ACGAGAGGTC AGAGAGCGTG 
CAGTACTTTG ATATGGAAGA CTGTGTGCTC ACAGCCATCG AACGGGACTT CATCTCCTGC 
CCCAGACACC CAGACCTCCC AGTACCACTT CAGGAGCTTG TACCAGAGCT GTTCATGACT 
GACTTCCCAG CCAGGCTCTT CCTGGAGAAC AGCAAGCTGG AGCACACGGA GGGTGAGAAC 
AGCATTCTGG GCCAAGGTGG CAGTGGCACA GTCATCTACC AGGCCCGGTA CCAGGGCCAG 
CCTGTGGCTG TGAAGAGGTT CCATATCAAG AAGTTCAAGA ATTCTGCTAA TGCCCCAGCA 
GACACCATGC TGCGGCACCT GAGAGCCATG GATGCCATGA AGAACTTCTC AGATTTCCGT 
CAGGAGGCCA GCATGCTGCA TGCCTTGCAG CATCCTTGCA TCGTGTCACT CATCGGCATC 
AGCATCCACC CGCTCTGCTT CGCCTTGGAG CTGGCCCCGC TGGGCAGTCT CAACACCGTG 
CTATCTGAGA ATGCCAAAGA TTCTTCCTTT ATGCCCCTGG GACACATGCT CACCCAAAAG 
ATAGCCTACC AGATTGCCTC GGGCCTGGCC TACCTGCACA AGAAAAACAT CATCTTCTGT 
GACCTCAAGT CGGACAACAT CCTAGTGTGG TCGCTGAGCG CCAAGGAGCA TATCAACATC 
AAACTGTCAG ACTACGGCAT CTCCAGGCAG TCCTTTCACG AGGGGGCCCT GGGTGTGGAG 
GGCACCCCAG GTTACCAGGC CCCAGAGATC AGGCCTCGCA TCGTGTATGA CGAGAAGGTA 
GATATGTTCT CCTACGGCAT GGTGCTATAC GAGCTGCTGT CAGGACAGCG TCCTGCACTG 
GGCCACCACC AACTTCAGAT TGTCAAGAAG TTGTCCAAGG GCATCCGCCC AGTTCTGGGG 
CAGCCGGAGG AAGTTCAGTT CCATCGGCTG CAGGCGCTCA TGATGGAGTG CTGGGACACA 
AAGCCGGAGA AGCGCCCCCT GGCCTTGTCC GTGGTGAGCC AGATGAAAGA CCCAACCTTT 
GCCACCTTCA TGTACATGCT GCCCTGTGGG AAGCAGTCGG CCTTCTTCTC ATCCCAGAGC 
CAGGAGTACA CTGTGGTGTT CTGGGATGGG AAGGAGGAGT CCAGGAATTA CACGGTGGTC 
AACACAGAGA AGGGCCTTCT GGAAGTACAG AGGATGACCT GCCCCGGGAT GAAGTTGAGC 
TGTCAGCTCA AGGTCCAGAG CTCAGTGTGG ATAGCCACGG AGGACCAGAA AATCTATATC 
TACAGCCTTA AGGGTATGTG CCCATTAAGC GTGCCCCAGC AGGCCCTGGA CACCCCAGCT 
GTCGTCACCT GTTTCTTGGC AGTACCTGTT ATCAAAAAGA ACTCCTTCCT GGTGCTGGCA 
GGCCTGGCTG ACGGACTCGT GGCTGTGTTT CCTGTGGCAC GGGGCACCCC AAAAGAAAGC 
TGCTCCTACC TGTGCTCCCA CACAGCCAAC AGGTCCAAGT TCTGCATCCC CGACGAAGAT 
GCACGGCAGA ACCCTTACCC AGTGAAGGCA ATGGAAGTGG TCAACAGTGG GTCTGAGGTC 
TGGTACAGCA ACGGGCCTGG CCTCCTCGTC ATTGACTGCA CCATCCTGGA CATCAGCAGG 
CGGCTGGAGC CCTACGCAGC TCCGTCCATG GTCACGTCAC TTGTGTGCAG CTCAGACTGC 
AGAGGAGAGG AGATGGTCTG GTGCCTCGAC GACAAGGCCA ATTGCTTAGT GATGTACCAC 
TCAGCCACCT ACCAGCTCTG TGCCCGGTAC TTCTGTGGGG ACCCCAATCC TCTCAGGGAC 
ACGTTTTCTG TGCAGCCTTC AGTCCTAGAA ACCCCAGGCA GTCACAAAAC CACCTCAAAG 
GGGCCTGTGG AAGAGTGCAT CGCGGATGTG AGCATCATGT ACAGCGAGGA GCTGGGCACG 
CAGATCCTCA CCCACCAGGA CTCGCTCACC GACTACTGCT CCATGTCCTC TTATTCTTCG 
TCCCCACCCC ACCAAGATCC TAGGTCCCCT TCCAGTCTGC CCAGCTCCCT CACCAGCTAT 
TCCAGTGTGC CTTTCTCTGC CAACTATGAA GACTCAGACA GGCTGCAGGA GCCCAGTGTC 
ACCTCTGACA GAACTGAGCA TGACCTGAGC CCCATGGATG GGGAGACGTT CAGCCAGCAC 
CTGCAGGCTG TGAAGGTGCT TGCTGTCAAA GACCTCATTT GGGTTCCTAG ACATGGAGGA 
GATATCATTG TCATCGGCCT GGAGAAGGAT TCAGGTGCCC AGCGGGGCAG AGTCATCGCT 
GTTTTAAAAG CCCGAGAGCT CAATCGGCAC GGGGTTCTGG TGGATGCCGC AGTGGTGGCA 
AAGGACACCG TAGTGTGCAG CTTTGCAAAT GAAAACACAG AGTGGTGCCT GGCTGTCTGG 
AGGGGCTGGG GCGCCAGGGA GTTTGACATC TTCTACCAGT CCTATGAGGA GCTGGGCCGG 
CTGGAGGCTT GTACTCGCAA GAGAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrk1 ( XM_006540839.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540902.1 Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu38811
Clone ID Related Accession (Same CDS sequence)	XM_006540839.3 , XM_006540839.4
Accession Version	XM_006540839.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006540839.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAA GAAGATGGAA CATCCCAGAG GGGCTTCAAA TCCCAGGTGC CGAAGACATG 
GGCAGCAAAC TGTCTTTACC AGGTGGCAGC TCCACGGTGC AGTGCCCCAG CATGGAAGAA 
ATCCACACTG CGTACAAGCA GAGGAACCTC TCCCGAGCCC GAGACCTACT CAGAGGGGTT 
TGTGAAGAGA GCGAATCCTC ACAGGAGAAG GGCCAGCTGC TGAGCATCGC GGCAGCCCAC 
GGGGATCTGG AGACTGTTCA GTTCCTGCTC ACTGAGAAGC GCGTGGAGCT GCCGACGGAG 
CCCACCGATG ACAACCCAGC TGTGGTGGCA GCGCATTTTG GACACGCCGA AGTAGTACGT 
GAACTACTAG AGTCCTTGCC AGGTCCCTGT ACCCCCCAGC GGCTACTGAA CTGGATGCTG 
GCCCTGGCTT GCCAGCGAGG GCACCTGGAG GTTGTGAAGC TGCTGGTCCT GACACATGGG 
GCTGACCCGG AGAACTATGC CGTCAGGAAG AATGAGTTCC CCGTCATTGT GCGGTTGCCC 
CTCTATGCGG CCATCAAGGC AGGGAATGAA GACATTGCCA TATTCCTGCT TCGGCACGGA 
GCCTATTTCT GCTCCTACAT CCTGCTGGAT AGTCCTGAGC CAAGCAAACA TCTGCTCAGG 
AAGTATTTCA TCGAAGCCAG CGCCCTGCCC AGCAGCTATC CCGGGAAAAT CGCACTTCGT 
GTGAAATGGT CCCATCTGAA GTTGCCCTGG GTGGACCTGG ACTGGCTCCT AGACATCTCC 
TGCCAGATCA CAGAGCTTGA CCTGTCTGCC AACTGCCTGC CTTCGCTCCC TTCCATCATC 
CCTTGGGGAC TCATCAATCT AAAGAAGCTG AATCTCTCAA ACAACCAGCT GGGGGAGCTG 
CCCTGCGTGC AGTCCTCCGA CGAAATCATC TGCTCCAGGT TACTTGAAAT TGACATCTCC 
AGCAACAAAC TGTCTCACCT CCCTCCTGGA TTCCTGCACC TCTCAAAACT TCAAAAGCTG 
ACTGCTTCCA AAAACTACCT GGAGCGGCTG TTTGAAGAAG AAAACGCCAC TAACTGGATC 
GGCCTGCGGA AGCTGCAAGA ACTCGATCTA GCTGACAACA GGCTGACAGA GCTCCCTGTC 
CAATTTATGC ACTCCTTCAA GTCTCTCACC TCTCTGAATG TCTCCAGGAA CAACCTGAAG 
AGCTTCCCGG ATCCCTGGTC CTGCCCTTTG AAATGTTGTA AAGCCTCCAA AAATGCCCTG 
GAATCGCTGC CAGACAAAAT GGCTGTCTTT TGGAAAAGCC ACCTTCGGGA TGCAGATTTC 
TCTGAGAACT CTCTGAAAGA GGTGCCCCTG GGATTGTTCC AGCTTGACGC CCTCATGTTC 
TTGAGGTTAC AGGGAAACCA GCTGTTGTCG CTGCCACCTC AAGAGAAGTG GACCTGCACC 
CAACTCAAGA CCCTGGACCT CTCCAGAAAC CAGCTCGGCA AAAATGAAGA TGGGCTTAAA 
ACAAAGAGGA TCTCCCTGTT CACCACCAGA GGGCGCCAGC GTTCTGGAAC TGAGACAGCC 
TCCATGTTGG AATTCCCAGC TTTCCTAAGT GAGTCTTTGG AGGTCCTTTG TCTGAATGAC 
AACCATCTTG ATGCTGTTCC CCCATCGGTC TGCCTGCTGA AGAACCTCTC AGAGCTCTAC 
TTGGGCAATA ACCCTGGCCT CCGAGAGCTC CCTCCGGAGC TAGGCCAGCT GGGGAACCTC 
TGGCAACTGG ACATTGAAGA TCTGAACATT AGCAACGTGC CTGCGGAGGT CAGGAAAGAA 
GGCCCGAAAG CAACACTGTC TTTCCTGCGT GCTCAGCTGC GCAAAGCAGA GAAGTGTAAG 
CTGATGAAGA TGATCCTCGT GGGTCCCCCG CGCCAGGGCA AGTCCACACT CCTGGAGATC 
TTACAGACCG GGAAGGCCCC GCAGCTAGCA CACAGTGAAG CCACCATCAG GACCACCAAG 
TGGGAGCTCC AGAGACCAGC AGGCTCCAAA GCCAAGGTTG AGTCCGTAGA GTTCAACGTC 
TGGGACATCG GGGGTCCGGC CAGCATGGCC ACGGTCAATC AGTGCTTCTT CACAGACAAG 
GCGCTGTATG TGGTGGTCTG GAACCTGGCA CTGGGAGAGG AAGCCGTGGC CAACCTCCAG 
TTCTGGCTGC TCAACATTGA GGCCAAGGCC CCAAATGCCG TTGTGCTGGT GGTCGGGACA 
CACCTGGACC TAATCGAAGC TAAGTTCCGA GTGGAGAGGA TAGCAACCCT GCGTGCCTAC 
GTGCTGGCCC TCTGCCGCTC GCCATCGGGC TCCAGGGCTA CGGGCTTCCC GGACATCACT 
TTCAAACACT TGCATGAGAT CTCCTGCAAG AATCTGGAAG GGCAGGAGGG GCTGCGGCAA 
CTGATCTTCC ACGTCACGTG CAACATGAAG GATGTGGGTA GCACTATCGG CTGTCAAAAA 
CTCGCCGGCA GGCTGATCCC CAGGAGCTAC ATAAGCCTAC AGGAGGCCGT GCTGGCTGAG 
CAACAGCGCC GCAGTCTGGG TGACCAAGTA CAGTATCTGA CGGACAGGCA GCTGGACCAG 
CTGGTAGAGC AGACGCCTGG CAATGATATC AAAGACTATG AAGACCTGCA GTCTGCCATC 
AGCTTCCTCA TAGAAACTGG GACCCTGCTG CACTTTCCAG ACACGAGCCA TGGCCTAAGG 
AACCTCTACT TCCTGGATCC CATCTGGCTC TCTGAATGCC TGCAGAGGAT CTTTAACATC 
AAGGGCTCGC GGTCAGTGGC AAAGAATGGG GTGATCCAAG CGGAAGACCT CAGGATGCTG 
CTGGTGGGGA CAGGCTTCAC ACAGCAGACT GAGGAGCAAT ACTTCCAGTT CCTTGCCAAG 
TTCGAGATTG CCCTCCCTGT TGCCAATGAC AGCTACCTCC TACCACACCT CCTTCCATCC 
AAGCCTGGGC TGGACACCCA CAGCATGCGG CACCCAATGG CTAACACCAT CCAGCGGGTG 
TTTAAGATGA GCTTCGTGCC TGTTGGCTTC TGGCAACGGT TCATAGCACG GATGCTAATC 
AGCTTGGCTG AGATGGACCT GCAGCTTTTT GAAAACAAGA AGAATACGAA AAGCAGGAAC 
CGGAAAGTCA CTATTTACAG TTTTACAGGG AGCCAGAGAA ACCGCTGCAG TACATTCCGA 
GTCCGAAGAA ATCAGACCAT CTACTGGCAG GAAGGGCTGC TGGTCACTTT CGATGGGGGC 
TACCTCAGTG TGGAATCCTC AGATGTGAAC TGGAAAAAGA AAAAAAGCGG AGGAATTAAA 
ATCATCTGCC AGTCAGAGAT GAGGGACTTC TCGGCAATGG CTTTTATCAC AGACCATGTC 
AACTCCCTGA TTGACCAGTG GTTCCCTGCC CTGACGGCCA CAGAAAGTGA CGGGACCCCA 
CTGATGGAGC AGTACGTACC CTGCCCTGTC TGTGAAGCAT CCTGGGCCCA GCATGCAGAT 
CCAAACGAGA GGTCAGAGAG CGTGCAGTAC TTTGATATGG AAGACTGTGT GCTCACAGCC 
ATCGAACGGG ACTTCATCTC CTGCCCCAGA CACCCAGACC TCCCAGTACC ACTTCAGGAG 
CTTGTACCAG AGCTGTTCAT GACTGACTTC CCAGCCAGGC TCTTCCTGGA GAACAGCAAG 
CTGGAGCACA CGGAGGGTGA GAACAGCATT CTGGGCCAAG GTGGCAGTGG CACAGTCATC 
TACCAGGCCC GGTACCAGGG CCAGCCTGTG GCTGTGAAGA GGTTCCATAT CAAGAAGTTC 
AAGAATTCTG CTAATGCCCC AGCAGACACC ATGCTGCGGC ACCTGAGAGC CATGGATGCC 
ATGAAGAACT TCTCAGATTT CCGTCAGGAG GCCAGCATGC TGCATGCCTT GCAGCATCCT 
TGCATCGTGT CACTCATCGG CATCAGCATC CACCCGCTCT GCTTCGCCTT GGAGCTGGCC 
CCGCTGGGCA GTCTCAACAC CGTGCTATCT GAGAATGCCA AAGATTCTTC CTTTATGCCC 
CTGGGACACA TGCTCACCCA AAAGATAGCC TACCAGATTG CCTCGGGCCT GGCCTACCTG 
CACAAGAAAA ACATCATCTT CTGTGACCTC AAGTCGGACA ACATCCTAGT GTGGTCGCTG 
AGCGCCAAGG AGCATATCAA CATCAAACTG TCAGACTACG GCATCTCCAG GCAGTCCTTT 
CACGAGGGGG CCCTGGGTGT GGAGGGCACC CCAGGTTACC AGGCCCCAGA GATCAGGCCT 
CGCATCGTGT ATGACGAGAA GGTAGATATG TTCTCCTACG GCATGGTGCT ATACGAGCTG 
CTGTCAGGAC AGCGTCCTGC ACTGGGCCAC CACCAACTTC AGATTGTCAA GAAGTTGTCC 
AAGGGCATCC GCCCAGTTCT GGGGCAGCCG GAGGAAGTTC AGTTCCATCG GCTGCAGGCG 
CTCATGATGG AGTGCTGGGA CACAAAGCCG GAGAAGCGCC CCCTGGCCTT GTCCGTGGTG 
AGCCAGATGA AAGACCCAAC CTTTGCCACC TTCATGTACA TGCTGCCCTG TGGGAAGCAG 
TCGGCCTTCT TCTCATCCCA GAGCCAGGAG TACACTGTGG TGTTCTGGGA TGGGAAGGAG 
GAGTCCAGGA ATTACACGGT GGTCAACACA GAGAAGGGCC TTCTGGAAGT ACAGAGGATG 
ACCTGCCCCG GGATGAAGTT GAGCTGTCAG CTCAAGGTCC AGAGCTCAGT GTGGATAGCC 
ACGGAGGACC AGAAAATCTA TATCTACAGC CTTAAGGGTA TGTGCCCATT AAGCGTGCCC 
CAGCAGGCCC TGGACACCCC AGCTGTCGTC ACCTGTTTCT TGGCAGTACC TGTTATCAAA 
AAGAACTCCT TCCTGGTGCT GGCAGGCCTG GCTGACGGAC TCGTGGCTGT GTTTCCTGTG 
GCACGGGGCA CCCCAAAAGA AAGCTGCTCC TACCTGTGCT CCCACACAGC CAACAGGTCC 
AAGTTCTGCA TCCCCGACGA AGATGCACGG CAGAACCCTT ACCCAGTGAA GGCAATGGAA 
GTGGTCAACA GTGGGTCTGA GGTCTGGTAC AGCAACGGGC CTGGCCTCCT CGTCATTGAC 
TGCACCATCC TGGACATCAG CAGGCGGCTG GAGCCCTACG CAGCTCCGTC CATGGTCACG 
TCACTTGTGT GCAGCTCAGA CTGCAGAGGA GAGGAGATGG TCTGGTGCCT CGACGACAAG 
GCCAATTGCT TAGTGATGTA CCACTCAGCC ACCTACCAGC TCTGTGCCCG GTACTTCTGT 
GGGGACCCCA ATCCTCTCAG GGACACGTTT TCTGTGCAGC CTTCAGTCCT AGAAACCCCA 
GGCAGTCACA AAACCACCTC AAAGGGGCCT GTGGAAGAGT GCATCGCGGA TGTGAGCATC 
ATGTACAGCG AGGAGCTGGG CACGCAGATC CTCACCCACC AGGACTCGCT CACCGACTAC 
TGCTCCATGT CCTCTTATTC TTCGTCCCCA CCCCACCAAG ATCCTAGGTC CCCTTCCAGT 
CTGCCCAGCT CCCTCACCAG CTATTCCAGT GTGCCTTTCT CTGCCAACTA TGAAGACTCA 
GACAGGCTGC AGGAGCCCAG TGTCACCTCT GACAGAACTG AGCATGACCT GAGCCCCATG 
GATGGGGAGA CGTTCAGCCA GCACCTGCAG GCTGTGAAGG TGCTTGCTGT CAAAGACCTC 
ATTTGGGTTC CTAGACATGG AGGAGATATC ATTGTCATCG GCCTGGAGAA GGATTCAGGT 
GCCCAGCGGG GCAGAGTCAT CGCTGTTTTA AAAGCCCGAG AGCTCAATCG GCACGGGGTT 
CTGGTGGATG CCGCAGTGGT GGCAAAGGAC ACCGTAGTGT GCAGCTTTGC AAATGAAAAC 
ACAGAGTGGT GCCTGGCTGT CTGGAGGGGC TGGGGCGCCA GGGAGTTTGA CATCTTCTAC 
CAGTCCTATG AGGAGCTGGG CCGGCTGGAG GCTTGTACTC GCAAGAGAAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540902.1
CDS	556..6549
Translation	MDQRRWNIPEGLQIPGAEDMGSKLSLPGGSSTVQCPSMEEIHTAYKQRNLSRARDLLRGVCEESESSQEKGQLLSIAAAHGDLETVQFLLTEKRVELPTEPTDDNPAVVAAHFGHAEVVRELLESLPGPCTPQRLLNWMLALACQRGHLEVVKLLVLTHGADPENYAVRKNEFPVIVRLPLYAAIKAGNEDIAIFLLRHGAYFCSYILLDSPEPSKHLLRKYFIEASALPSSYPGKIALRVKWSHLKLPWVDLDWLLDISCQITELDLSANCLPSLPSIIPWGLINLKKLNLSNNQLGELPCVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLERLFEEENATNWIGLRKLQELDLADNRLTELPVQFMHSFKSLTSLNVSRNNLKSFPDPWSCPLKCCKASKNALESLPDKMAVFWKSHLRDADFSENSLKEVPLGLFQLDALMFLRLQGNQLLSLPPQEKWTCTQLKTLDLSRNQLGKNEDGLKTKRISLFTTRGRQRSGTETASMLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKNLSELYLGNNPGLRELPPELGQLGNLWQLDIEDLNISNVPAEVRKEGPKATLSFLRAQLRKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQLAHSEATIRTTKWELQRPAGSKAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAGRLIPRSYISLQEAVLAEQQRRSLGDQVQYLTDRQLDQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIQAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQARYQGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLSAKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIVKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSAFFSSQSQEYTVVFWDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWIATEDQKIYIYSLKGMCPLSVPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFPVARGTPKESCSYLCSHTANRSKFCIPDEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCTILDISRRLEPYAAPSMVTSLVCSSDCRGEEMVWCLDDKANCLVMYHSATYQLCARYFCGDPNPLRDTFSVQPSVLETPGSHKTTSKGPVEECIADVSIMYSEELGTQILTHQDSLTDYCSMSSYSSSPPHQDPRSPSSLPSSLTSYSSVPFSANYEDSDRLQEPSVTSDRTEHDLSPMDGETFSQHLQAVKVLAVKDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNRHGVLVDAAVVAKDTVVCSFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR

Target ORF information:

RefSeq Version	XM_006540839.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540839.4

ORF Insert Sequence:

ATGGATCAAA GAAGATGGAA CATCCCAGAG GGGCTTCAAA TCCCAGGTGC CGAAGACATG 
GGCAGCAAAC TGTCTTTACC AGGTGGCAGC TCCACGGTGC AGTGCCCCAG CATGGAAGAA 
ATCCACACTG CGTACAAGCA GAGGAACCTC TCCCGAGCCC GAGACCTACT CAGAGGGGTT 
TGTGAAGAGA GCGAATCCTC ACAGGAGAAG GGCCAGCTGC TGAGCATCGC GGCAGCCCAC 
GGGGATCTGG AGACTGTTCA GTTCCTGCTC ACTGAGAAGC GCGTGGAGCT GCCGACGGAG 
CCCACCGATG ACAACCCAGC TGTGGTGGCA GCGCATTTTG GACACGCCGA AGTAGTACGT 
GAACTACTAG AGTCCTTGCC AGGTCCCTGT ACCCCCCAGC GGCTACTGAA CTGGATGCTG 
GCCCTGGCTT GCCAGCGAGG GCACCTGGAG GTTGTGAAGC TGCTGGTCCT GACACATGGG 
GCTGACCCGG AGAACTATGC CGTCAGGAAG AATGAGTTCC CCGTCATTGT GCGGTTGCCC 
CTCTATGCGG CCATCAAGGC AGGGAATGAA GACATTGCCA TATTCCTGCT TCGGCACGGA 
GCCTATTTCT GCTCCTACAT CCTGCTGGAT AGTCCTGAGC CAAGCAAACA TCTGCTCAGG 
AAGTATTTCA TCGAAGCCAG CGCCCTGCCC AGCAGCTATC CCGGGAAAAT CGCACTTCGT 
GTGAAATGGT CCCATCTGAA GTTGCCCTGG GTGGACCTGG ACTGGCTCCT AGACATCTCC 
TGCCAGATCA CAGAGCTTGA CCTGTCTGCC AACTGCCTGC CTTCGCTCCC TTCCATCATC 
CCTTGGGGAC TCATCAATCT AAAGAAGCTG AATCTCTCAA ACAACCAGCT GGGGGAGCTG 
CCCTGCGTGC AGTCCTCCGA CGAAATCATC TGCTCCAGGT TACTTGAAAT TGACATCTCC 
AGCAACAAAC TGTCTCACCT CCCTCCTGGA TTCCTGCACC TCTCAAAACT TCAAAAGCTG 
ACTGCTTCCA AAAACTACCT GGAGCGGCTG TTTGAAGAAG AAAACGCCAC TAACTGGATC 
GGCCTGCGGA AGCTGCAAGA ACTCGATCTA GCTGACAACA GGCTGACAGA GCTCCCTGTC 
CAATTTATGC ACTCCTTCAA GTCTCTCACC TCTCTGAATG TCTCCAGGAA CAACCTGAAG 
AGCTTCCCGG ATCCCTGGTC CTGCCCTTTG AAATGTTGTA AAGCCTCCAA AAATGCCCTG 
GAATCGCTGC CAGACAAAAT GGCTGTCTTT TGGAAAAGCC ACCTTCGGGA TGCAGATTTC 
TCTGAGAACT CTCTGAAAGA GGTGCCCCTG GGATTGTTCC AGCTTGACGC CCTCATGTTC 
TTGAGGTTAC AGGGAAACCA GCTGTTGTCG CTGCCACCTC AAGAGAAGTG GACCTGCACC 
CAACTCAAGA CCCTGGACCT CTCCAGAAAC CAGCTCGGCA AAAATGAAGA TGGGCTTAAA 
ACAAAGAGGA TCTCCCTGTT CACCACCAGA GGGCGCCAGC GTTCTGGAAC TGAGACAGCC 
TCCATGTTGG AATTCCCAGC TTTCCTAAGT GAGTCTTTGG AGGTCCTTTG TCTGAATGAC 
AACCATCTTG ATGCTGTTCC CCCATCGGTC TGCCTGCTGA AGAACCTCTC AGAGCTCTAC 
TTGGGCAATA ACCCTGGCCT CCGAGAGCTC CCTCCGGAGC TAGGCCAGCT GGGGAACCTC 
TGGCAACTGG ACATTGAAGA TCTGAACATT AGCAACGTGC CTGCGGAGGT CAGGAAAGAA 
GGCCCGAAAG CAACACTGTC TTTCCTGCGT GCTCAGCTGC GCAAAGCAGA GAAGTGTAAG 
CTGATGAAGA TGATCCTCGT GGGTCCCCCG CGCCAGGGCA AGTCCACACT CCTGGAGATC 
TTACAGACCG GGAAGGCCCC GCAGCTAGCA CACAGTGAAG CCACCATCAG GACCACCAAG 
TGGGAGCTCC AGAGACCAGC AGGCTCCAAA GCCAAGGTTG AGTCCGTAGA GTTCAACGTC 
TGGGACATCG GGGGTCCGGC CAGCATGGCC ACGGTCAATC AGTGCTTCTT CACAGACAAG 
GCGCTGTATG TGGTGGTCTG GAACCTGGCA CTGGGAGAGG AAGCCGTGGC CAACCTCCAG 
TTCTGGCTGC TCAACATTGA GGCCAAGGCC CCAAATGCCG TTGTGCTGGT GGTCGGGACA 
CACCTGGACC TAATCGAAGC TAAGTTCCGA GTGGAGAGGA TAGCAACCCT GCGTGCCTAC 
GTGCTGGCCC TCTGCCGCTC GCCATCGGGC TCCAGGGCTA CGGGCTTCCC GGACATCACT 
TTCAAACACT TGCATGAGAT CTCCTGCAAG AATCTGGAAG GGCAGGAGGG GCTGCGGCAA 
CTGATCTTCC ACGTCACGTG CAACATGAAG GATGTGGGTA GCACTATCGG CTGTCAAAAA 
CTCGCCGGCA GGCTGATCCC CAGGAGCTAC ATAAGCCTAC AGGAGGCCGT GCTGGCTGAG 
CAACAGCGCC GCAGTCTGGG TGACCAAGTA CAGTATCTGA CGGACAGGCA GCTGGACCAG 
CTGGTAGAGC AGACGCCTGG CAATGATATC AAAGACTATG AAGACCTGCA GTCTGCCATC 
AGCTTCCTCA TAGAAACTGG GACCCTGCTG CACTTTCCAG ACACGAGCCA TGGCCTAAGG 
AACCTCTACT TCCTGGATCC CATCTGGCTC TCTGAATGCC TGCAGAGGAT CTTTAACATC 
AAGGGCTCGC GGTCAGTGGC AAAGAATGGG GTGATCCAAG CGGAAGACCT CAGGATGCTG 
CTGGTGGGGA CAGGCTTCAC ACAGCAGACT GAGGAGCAAT ACTTCCAGTT CCTTGCCAAG 
TTCGAGATTG CCCTCCCTGT TGCCAATGAC AGCTACCTCC TACCACACCT CCTTCCATCC 
AAGCCTGGGC TGGACACCCA CAGCATGCGG CACCCAATGG CTAACACCAT CCAGCGGGTG 
TTTAAGATGA GCTTCGTGCC TGTTGGCTTC TGGCAACGGT TCATAGCACG GATGCTAATC 
AGCTTGGCTG AGATGGACCT GCAGCTTTTT GAAAACAAGA AGAATACGAA AAGCAGGAAC 
CGGAAAGTCA CTATTTACAG TTTTACAGGG AGCCAGAGAA ACCGCTGCAG TACATTCCGA 
GTCCGAAGAA ATCAGACCAT CTACTGGCAG GAAGGGCTGC TGGTCACTTT CGATGGGGGC 
TACCTCAGTG TGGAATCCTC AGATGTGAAC TGGAAAAAGA AAAAAAGCGG AGGAATTAAA 
ATCATCTGCC AGTCAGAGAT GAGGGACTTC TCGGCAATGG CTTTTATCAC AGACCATGTC 
AACTCCCTGA TTGACCAGTG GTTCCCTGCC CTGACGGCCA CAGAAAGTGA CGGGACCCCA 
CTGATGGAGC AGTACGTACC CTGCCCTGTC TGTGAAGCAT CCTGGGCCCA GCATGCAGAT 
CCAAACGAGA GGTCAGAGAG CGTGCAGTAC TTTGATATGG AAGACTGTGT GCTCACAGCC 
ATCGAACGGG ACTTCATCTC CTGCCCCAGA CACCCAGACC TCCCAGTACC ACTTCAGGAG 
CTTGTACCAG AGCTGTTCAT GACTGACTTC CCAGCCAGGC TCTTCCTGGA GAACAGCAAG 
CTGGAGCACA CGGAGGGTGA GAACAGCATT CTGGGCCAAG GTGGCAGTGG CACAGTCATC 
TACCAGGCCC GGTACCAGGG CCAGCCTGTG GCTGTGAAGA GGTTCCATAT CAAGAAGTTC 
AAGAATTCTG CTAATGCCCC AGCAGACACC ATGCTGCGGC ACCTGAGAGC CATGGATGCC 
ATGAAGAACT TCTCAGATTT CCGTCAGGAG GCCAGCATGC TGCATGCCTT GCAGCATCCT 
TGCATCGTGT CACTCATCGG CATCAGCATC CACCCGCTCT GCTTCGCCTT GGAGCTGGCC 
CCGCTGGGCA GTCTCAACAC CGTGCTATCT GAGAATGCCA AAGATTCTTC CTTTATGCCC 
CTGGGACACA TGCTCACCCA AAAGATAGCC TACCAGATTG CCTCGGGCCT GGCCTACCTG 
CACAAGAAAA ACATCATCTT CTGTGACCTC AAGTCGGACA ACATCCTAGT GTGGTCGCTG 
AGCGCCAAGG AGCATATCAA CATCAAACTG TCAGACTACG GCATCTCCAG GCAGTCCTTT 
CACGAGGGGG CCCTGGGTGT GGAGGGCACC CCAGGTTACC AGGCCCCAGA GATCAGGCCT 
CGCATCGTGT ATGACGAGAA GGTAGATATG TTCTCCTACG GCATGGTGCT ATACGAGCTG 
CTGTCAGGAC AGCGTCCTGC ACTGGGCCAC CACCAACTTC AGATTGTCAA GAAGTTGTCC 
AAGGGCATCC GCCCAGTTCT GGGGCAGCCG GAGGAAGTTC AGTTCCATCG GCTGCAGGCG 
CTCATGATGG AGTGCTGGGA CACAAAGCCG GAGAAGCGCC CCCTGGCCTT GTCCGTGGTG 
AGCCAGATGA AAGACCCAAC CTTTGCCACC TTCATGTACA TGCTGCCCTG TGGGAAGCAG 
TCGGCCTTCT TCTCATCCCA GAGCCAGGAG TACACTGTGG TGTTCTGGGA TGGGAAGGAG 
GAGTCCAGGA ATTACACGGT GGTCAACACA GAGAAGGGCC TTCTGGAAGT ACAGAGGATG 
ACCTGCCCCG GGATGAAGTT GAGCTGTCAG CTCAAGGTCC AGAGCTCAGT GTGGATAGCC 
ACGGAGGACC AGAAAATCTA TATCTACAGC CTTAAGGGTA TGTGCCCATT AAGCGTGCCC 
CAGCAGGCCC TGGACACCCC AGCTGTCGTC ACCTGTTTCT TGGCAGTACC TGTTATCAAA 
AAGAACTCCT TCCTGGTGCT GGCAGGCCTG GCTGACGGAC TCGTGGCTGT GTTTCCTGTG 
GCACGGGGCA CCCCAAAAGA AAGCTGCTCC TACCTGTGCT CCCACACAGC CAACAGGTCC 
AAGTTCTGCA TCCCCGACGA AGATGCACGG CAGAACCCTT ACCCAGTGAA GGCAATGGAA 
GTGGTCAACA GTGGGTCTGA GGTCTGGTAC AGCAACGGGC CTGGCCTCCT CGTCATTGAC 
TGCACCATCC TGGACATCAG CAGGCGGCTG GAGCCCTACG CAGCTCCGTC CATGGTCACG 
TCACTTGTGT GCAGCTCAGA CTGCAGAGGA GAGGAGATGG TCTGGTGCCT CGACGACAAG 
GCCAATTGCT TAGTGATGTA CCACTCAGCC ACCTACCAGC TCTGTGCCCG GTACTTCTGT 
GGGGACCCCA ATCCTCTCAG GGACACGTTT TCTGTGCAGC CTTCAGTCCT AGAAACCCCA 
GGCAGTCACA AAACCACCTC AAAGGGGCCT GTGGAAGAGT GCATCGCGGA TGTGAGCATC 
ATGTACAGCG AGGAGCTGGG CACGCAGATC CTCACCCACC AGGACTCGCT CACCGACTAC 
TGCTCCATGT CCTCTTATTC TTCGTCCCCA CCCCACCAAG ATCCTAGGTC CCCTTCCAGT 
CTGCCCAGCT CCCTCACCAG CTATTCCAGT GTGCCTTTCT CTGCCAACTA TGAAGACTCA 
GACAGGCTGC AGGAGCCCAG TGTCACCTCT GACAGAACTG AGCATGACCT GAGCCCCATG 
GATGGGGAGA CGTTCAGCCA GCACCTGCAG GCTGTGAAGG TGCTTGCTGT CAAAGACCTC 
ATTTGGGTTC CTAGACATGG AGGAGATATC ATTGTCATCG GCCTGGAGAA GGATTCAGGT 
GCCCAGCGGG GCAGAGTCAT CGCTGTTTTA AAAGCCCGAG AGCTCAATCG GCACGGGGTT 
CTGGTGGATG CCGCAGTGGT GGCAAAGGAC ACCGTAGTGT GCAGCTTTGC AAATGAAAAC 
ACAGAGTGGT GCCTGGCTGT CTGGAGGGGC TGGGGCGCCA GGGAGTTTGA CATCTTCTAC 
CAGTCCTATG AGGAGCTGGG CCGGCTGGAG GCTTGTACTC GCAAGAGAAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrk1 ( XM_006540839.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540902.1 Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X2, mRNA.

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CloneID	OMu38811
Clone ID Related Accession (Same CDS sequence)	XM_006540839.3 , XM_006540839.4
Accession Version	XM_006540839.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCAAA GAAGATGGAA CATCCCAGAG GGGCTTCAAA TCCCAGGTGC CGAAGACATG 
GGCAGCAAAC TGTCTTTACC AGGTGGCAGC TCCACGGTGC AGTGCCCCAG CATGGAAGAA 
ATCCACACTG CGTACAAGCA GAGGAACCTC TCCCGAGCCC GAGACCTACT CAGAGGGGTT 
TGTGAAGAGA GCGAATCCTC ACAGGAGAAG GGCCAGCTGC TGAGCATCGC GGCAGCCCAC 
GGGGATCTGG AGACTGTTCA GTTCCTGCTC ACTGAGAAGC GCGTGGAGCT GCCGACGGAG 
CCCACCGATG ACAACCCAGC TGTGGTGGCA GCGCATTTTG GACACGCCGA AGTAGTACGT 
GAACTACTAG AGTCCTTGCC AGGTCCCTGT ACCCCCCAGC GGCTACTGAA CTGGATGCTG 
GCCCTGGCTT GCCAGCGAGG GCACCTGGAG GTTGTGAAGC TGCTGGTCCT GACACATGGG 
GCTGACCCGG AGAACTATGC CGTCAGGAAG AATGAGTTCC CCGTCATTGT GCGGTTGCCC 
CTCTATGCGG CCATCAAGGC AGGGAATGAA GACATTGCCA TATTCCTGCT TCGGCACGGA 
GCCTATTTCT GCTCCTACAT CCTGCTGGAT AGTCCTGAGC CAAGCAAACA TCTGCTCAGG 
AAGTATTTCA TCGAAGCCAG CGCCCTGCCC AGCAGCTATC CCGGGAAAAT CGCACTTCGT 
GTGAAATGGT CCCATCTGAA GTTGCCCTGG GTGGACCTGG ACTGGCTCCT AGACATCTCC 
TGCCAGATCA CAGAGCTTGA CCTGTCTGCC AACTGCCTGC CTTCGCTCCC TTCCATCATC 
CCTTGGGGAC TCATCAATCT AAAGAAGCTG AATCTCTCAA ACAACCAGCT GGGGGAGCTG 
CCCTGCGTGC AGTCCTCCGA CGAAATCATC TGCTCCAGGT TACTTGAAAT TGACATCTCC 
AGCAACAAAC TGTCTCACCT CCCTCCTGGA TTCCTGCACC TCTCAAAACT TCAAAAGCTG 
ACTGCTTCCA AAAACTACCT GGAGCGGCTG TTTGAAGAAG AAAACGCCAC TAACTGGATC 
GGCCTGCGGA AGCTGCAAGA ACTCGATCTA GCTGACAACA GGCTGACAGA GCTCCCTGTC 
CAATTTATGC ACTCCTTCAA GTCTCTCACC TCTCTGAATG TCTCCAGGAA CAACCTGAAG 
AGCTTCCCGG ATCCCTGGTC CTGCCCTTTG AAATGTTGTA AAGCCTCCAA AAATGCCCTG 
GAATCGCTGC CAGACAAAAT GGCTGTCTTT TGGAAAAGCC ACCTTCGGGA TGCAGATTTC 
TCTGAGAACT CTCTGAAAGA GGTGCCCCTG GGATTGTTCC AGCTTGACGC CCTCATGTTC 
TTGAGGTTAC AGGGAAACCA GCTGTTGTCG CTGCCACCTC AAGAGAAGTG GACCTGCACC 
CAACTCAAGA CCCTGGACCT CTCCAGAAAC CAGCTCGGCA AAAATGAAGA TGGGCTTAAA 
ACAAAGAGGA TCTCCCTGTT CACCACCAGA GGGCGCCAGC GTTCTGGAAC TGAGACAGCC 
TCCATGTTGG AATTCCCAGC TTTCCTAAGT GAGTCTTTGG AGGTCCTTTG TCTGAATGAC 
AACCATCTTG ATGCTGTTCC CCCATCGGTC TGCCTGCTGA AGAACCTCTC AGAGCTCTAC 
TTGGGCAATA ACCCTGGCCT CCGAGAGCTC CCTCCGGAGC TAGGCCAGCT GGGGAACCTC 
TGGCAACTGG ACATTGAAGA TCTGAACATT AGCAACGTGC CTGCGGAGGT CAGGAAAGAA 
GGCCCGAAAG CAACACTGTC TTTCCTGCGT GCTCAGCTGC GCAAAGCAGA GAAGTGTAAG 
CTGATGAAGA TGATCCTCGT GGGTCCCCCG CGCCAGGGCA AGTCCACACT CCTGGAGATC 
TTACAGACCG GGAAGGCCCC GCAGCTAGCA CACAGTGAAG CCACCATCAG GACCACCAAG 
TGGGAGCTCC AGAGACCAGC AGGCTCCAAA GCCAAGGTTG AGTCCGTAGA GTTCAACGTC 
TGGGACATCG GGGGTCCGGC CAGCATGGCC ACGGTCAATC AGTGCTTCTT CACAGACAAG 
GCGCTGTATG TGGTGGTCTG GAACCTGGCA CTGGGAGAGG AAGCCGTGGC CAACCTCCAG 
TTCTGGCTGC TCAACATTGA GGCCAAGGCC CCAAATGCCG TTGTGCTGGT GGTCGGGACA 
CACCTGGACC TAATCGAAGC TAAGTTCCGA GTGGAGAGGA TAGCAACCCT GCGTGCCTAC 
GTGCTGGCCC TCTGCCGCTC GCCATCGGGC TCCAGGGCTA CGGGCTTCCC GGACATCACT 
TTCAAACACT TGCATGAGAT CTCCTGCAAG AATCTGGAAG GGCAGGAGGG GCTGCGGCAA 
CTGATCTTCC ACGTCACGTG CAACATGAAG GATGTGGGTA GCACTATCGG CTGTCAAAAA 
CTCGCCGGCA GGCTGATCCC CAGGAGCTAC ATAAGCCTAC AGGAGGCCGT GCTGGCTGAG 
CAACAGCGCC GCAGTCTGGG TGACCAAGTA CAGTATCTGA CGGACAGGCA GCTGGACCAG 
CTGGTAGAGC AGACGCCTGG CAATGATATC AAAGACTATG AAGACCTGCA GTCTGCCATC 
AGCTTCCTCA TAGAAACTGG GACCCTGCTG CACTTTCCAG ACACGAGCCA TGGCCTAAGG 
AACCTCTACT TCCTGGATCC CATCTGGCTC TCTGAATGCC TGCAGAGGAT CTTTAACATC 
AAGGGCTCGC GGTCAGTGGC AAAGAATGGG GTGATCCAAG CGGAAGACCT CAGGATGCTG 
CTGGTGGGGA CAGGCTTCAC ACAGCAGACT GAGGAGCAAT ACTTCCAGTT CCTTGCCAAG 
TTCGAGATTG CCCTCCCTGT TGCCAATGAC AGCTACCTCC TACCACACCT CCTTCCATCC 
AAGCCTGGGC TGGACACCCA CAGCATGCGG CACCCAATGG CTAACACCAT CCAGCGGGTG 
TTTAAGATGA GCTTCGTGCC TGTTGGCTTC TGGCAACGGT TCATAGCACG GATGCTAATC 
AGCTTGGCTG AGATGGACCT GCAGCTTTTT GAAAACAAGA AGAATACGAA AAGCAGGAAC 
CGGAAAGTCA CTATTTACAG TTTTACAGGG AGCCAGAGAA ACCGCTGCAG TACATTCCGA 
GTCCGAAGAA ATCAGACCAT CTACTGGCAG GAAGGGCTGC TGGTCACTTT CGATGGGGGC 
TACCTCAGTG TGGAATCCTC AGATGTGAAC TGGAAAAAGA AAAAAAGCGG AGGAATTAAA 
ATCATCTGCC AGTCAGAGAT GAGGGACTTC TCGGCAATGG CTTTTATCAC AGACCATGTC 
AACTCCCTGA TTGACCAGTG GTTCCCTGCC CTGACGGCCA CAGAAAGTGA CGGGACCCCA 
CTGATGGAGC AGTACGTACC CTGCCCTGTC TGTGAAGCAT CCTGGGCCCA GCATGCAGAT 
CCAAACGAGA GGTCAGAGAG CGTGCAGTAC TTTGATATGG AAGACTGTGT GCTCACAGCC 
ATCGAACGGG ACTTCATCTC CTGCCCCAGA CACCCAGACC TCCCAGTACC ACTTCAGGAG 
CTTGTACCAG AGCTGTTCAT GACTGACTTC CCAGCCAGGC TCTTCCTGGA GAACAGCAAG 
CTGGAGCACA CGGAGGGTGA GAACAGCATT CTGGGCCAAG GTGGCAGTGG CACAGTCATC 
TACCAGGCCC GGTACCAGGG CCAGCCTGTG GCTGTGAAGA GGTTCCATAT CAAGAAGTTC 
AAGAATTCTG CTAATGCCCC AGCAGACACC ATGCTGCGGC ACCTGAGAGC CATGGATGCC 
ATGAAGAACT TCTCAGATTT CCGTCAGGAG GCCAGCATGC TGCATGCCTT GCAGCATCCT 
TGCATCGTGT CACTCATCGG CATCAGCATC CACCCGCTCT GCTTCGCCTT GGAGCTGGCC 
CCGCTGGGCA GTCTCAACAC CGTGCTATCT GAGAATGCCA AAGATTCTTC CTTTATGCCC 
CTGGGACACA TGCTCACCCA AAAGATAGCC TACCAGATTG CCTCGGGCCT GGCCTACCTG 
CACAAGAAAA ACATCATCTT CTGTGACCTC AAGTCGGACA ACATCCTAGT GTGGTCGCTG 
AGCGCCAAGG AGCATATCAA CATCAAACTG TCAGACTACG GCATCTCCAG GCAGTCCTTT 
CACGAGGGGG CCCTGGGTGT GGAGGGCACC CCAGGTTACC AGGCCCCAGA GATCAGGCCT 
CGCATCGTGT ATGACGAGAA GGTAGATATG TTCTCCTACG GCATGGTGCT ATACGAGCTG 
CTGTCAGGAC AGCGTCCTGC ACTGGGCCAC CACCAACTTC AGATTGTCAA GAAGTTGTCC 
AAGGGCATCC GCCCAGTTCT GGGGCAGCCG GAGGAAGTTC AGTTCCATCG GCTGCAGGCG 
CTCATGATGG AGTGCTGGGA CACAAAGCCG GAGAAGCGCC CCCTGGCCTT GTCCGTGGTG 
AGCCAGATGA AAGACCCAAC CTTTGCCACC TTCATGTACA TGCTGCCCTG TGGGAAGCAG 
TCGGCCTTCT TCTCATCCCA GAGCCAGGAG TACACTGTGG TGTTCTGGGA TGGGAAGGAG 
GAGTCCAGGA ATTACACGGT GGTCAACACA GAGAAGGGCC TTCTGGAAGT ACAGAGGATG 
ACCTGCCCCG GGATGAAGTT GAGCTGTCAG CTCAAGGTCC AGAGCTCAGT GTGGATAGCC 
ACGGAGGACC AGAAAATCTA TATCTACAGC CTTAAGGGTA TGTGCCCATT AAGCGTGCCC 
CAGCAGGCCC TGGACACCCC AGCTGTCGTC ACCTGTTTCT TGGCAGTACC TGTTATCAAA 
AAGAACTCCT TCCTGGTGCT GGCAGGCCTG GCTGACGGAC TCGTGGCTGT GTTTCCTGTG 
GCACGGGGCA CCCCAAAAGA AAGCTGCTCC TACCTGTGCT CCCACACAGC CAACAGGTCC 
AAGTTCTGCA TCCCCGACGA AGATGCACGG CAGAACCCTT ACCCAGTGAA GGCAATGGAA 
GTGGTCAACA GTGGGTCTGA GGTCTGGTAC AGCAACGGGC CTGGCCTCCT CGTCATTGAC 
TGCACCATCC TGGACATCAG CAGGCGGCTG GAGCCCTACG CAGCTCCGTC CATGGTCACG 
TCACTTGTGT GCAGCTCAGA CTGCAGAGGA GAGGAGATGG TCTGGTGCCT CGACGACAAG 
GCCAATTGCT TAGTGATGTA CCACTCAGCC ACCTACCAGC TCTGTGCCCG GTACTTCTGT 
GGGGACCCCA ATCCTCTCAG GGACACGTTT TCTGTGCAGC CTTCAGTCCT AGAAACCCCA 
GGCAGTCACA AAACCACCTC AAAGGGGCCT GTGGAAGAGT GCATCGCGGA TGTGAGCATC 
ATGTACAGCG AGGAGCTGGG CACGCAGATC CTCACCCACC AGGACTCGCT CACCGACTAC 
TGCTCCATGT CCTCTTATTC TTCGTCCCCA CCCCACCAAG ATCCTAGGTC CCCTTCCAGT 
CTGCCCAGCT CCCTCACCAG CTATTCCAGT GTGCCTTTCT CTGCCAACTA TGAAGACTCA 
GACAGGCTGC AGGAGCCCAG TGTCACCTCT GACAGAACTG AGCATGACCT GAGCCCCATG 
GATGGGGAGA CGTTCAGCCA GCACCTGCAG GCTGTGAAGG TGCTTGCTGT CAAAGACCTC 
ATTTGGGTTC CTAGACATGG AGGAGATATC ATTGTCATCG GCCTGGAGAA GGATTCAGGT 
GCCCAGCGGG GCAGAGTCAT CGCTGTTTTA AAAGCCCGAG AGCTCAATCG GCACGGGGTT 
CTGGTGGATG CCGCAGTGGT GGCAAAGGAC ACCGTAGTGT GCAGCTTTGC AAATGAAAAC 
ACAGAGTGGT GCCTGGCTGT CTGGAGGGGC TGGGGCGCCA GGGAGTTTGA CATCTTCTAC 
CAGTCCTATG AGGAGCTGGG CCGGCTGGAG GCTTGTACTC GCAAGAGAAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540902.1
CDS	558..6551
Translation	MDQRRWNIPEGLQIPGAEDMGSKLSLPGGSSTVQCPSMEEIHTAYKQRNLSRARDLLRGVCEESESSQEKGQLLSIAAAHGDLETVQFLLTEKRVELPTEPTDDNPAVVAAHFGHAEVVRELLESLPGPCTPQRLLNWMLALACQRGHLEVVKLLVLTHGADPENYAVRKNEFPVIVRLPLYAAIKAGNEDIAIFLLRHGAYFCSYILLDSPEPSKHLLRKYFIEASALPSSYPGKIALRVKWSHLKLPWVDLDWLLDISCQITELDLSANCLPSLPSIIPWGLINLKKLNLSNNQLGELPCVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLERLFEEENATNWIGLRKLQELDLADNRLTELPVQFMHSFKSLTSLNVSRNNLKSFPDPWSCPLKCCKASKNALESLPDKMAVFWKSHLRDADFSENSLKEVPLGLFQLDALMFLRLQGNQLLSLPPQEKWTCTQLKTLDLSRNQLGKNEDGLKTKRISLFTTRGRQRSGTETASMLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKNLSELYLGNNPGLRELPPELGQLGNLWQLDIEDLNISNVPAEVRKEGPKATLSFLRAQLRKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQLAHSEATIRTTKWELQRPAGSKAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAGRLIPRSYISLQEAVLAEQQRRSLGDQVQYLTDRQLDQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIQAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQARYQGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLSAKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIVKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSAFFSSQSQEYTVVFWDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWIATEDQKIYIYSLKGMCPLSVPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFPVARGTPKESCSYLCSHTANRSKFCIPDEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCTILDISRRLEPYAAPSMVTSLVCSSDCRGEEMVWCLDDKANCLVMYHSATYQLCARYFCGDPNPLRDTFSVQPSVLETPGSHKTTSKGPVEECIADVSIMYSEELGTQILTHQDSLTDYCSMSSYSSSPPHQDPRSPSSLPSSLTSYSSVPFSANYEDSDRLQEPSVTSDRTEHDLSPMDGETFSQHLQAVKVLAVKDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNRHGVLVDAAVVAKDTVVCSFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR

Target ORF information:

RefSeq Version	XM_006540839.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540839.3

ORF Insert Sequence:

ATGGATCAAA GAAGATGGAA CATCCCAGAG GGGCTTCAAA TCCCAGGTGC CGAAGACATG 
GGCAGCAAAC TGTCTTTACC AGGTGGCAGC TCCACGGTGC AGTGCCCCAG CATGGAAGAA 
ATCCACACTG CGTACAAGCA GAGGAACCTC TCCCGAGCCC GAGACCTACT CAGAGGGGTT 
TGTGAAGAGA GCGAATCCTC ACAGGAGAAG GGCCAGCTGC TGAGCATCGC GGCAGCCCAC 
GGGGATCTGG AGACTGTTCA GTTCCTGCTC ACTGAGAAGC GCGTGGAGCT GCCGACGGAG 
CCCACCGATG ACAACCCAGC TGTGGTGGCA GCGCATTTTG GACACGCCGA AGTAGTACGT 
GAACTACTAG AGTCCTTGCC AGGTCCCTGT ACCCCCCAGC GGCTACTGAA CTGGATGCTG 
GCCCTGGCTT GCCAGCGAGG GCACCTGGAG GTTGTGAAGC TGCTGGTCCT GACACATGGG 
GCTGACCCGG AGAACTATGC CGTCAGGAAG AATGAGTTCC CCGTCATTGT GCGGTTGCCC 
CTCTATGCGG CCATCAAGGC AGGGAATGAA GACATTGCCA TATTCCTGCT TCGGCACGGA 
GCCTATTTCT GCTCCTACAT CCTGCTGGAT AGTCCTGAGC CAAGCAAACA TCTGCTCAGG 
AAGTATTTCA TCGAAGCCAG CGCCCTGCCC AGCAGCTATC CCGGGAAAAT CGCACTTCGT 
GTGAAATGGT CCCATCTGAA GTTGCCCTGG GTGGACCTGG ACTGGCTCCT AGACATCTCC 
TGCCAGATCA CAGAGCTTGA CCTGTCTGCC AACTGCCTGC CTTCGCTCCC TTCCATCATC 
CCTTGGGGAC TCATCAATCT AAAGAAGCTG AATCTCTCAA ACAACCAGCT GGGGGAGCTG 
CCCTGCGTGC AGTCCTCCGA CGAAATCATC TGCTCCAGGT TACTTGAAAT TGACATCTCC 
AGCAACAAAC TGTCTCACCT CCCTCCTGGA TTCCTGCACC TCTCAAAACT TCAAAAGCTG 
ACTGCTTCCA AAAACTACCT GGAGCGGCTG TTTGAAGAAG AAAACGCCAC TAACTGGATC 
GGCCTGCGGA AGCTGCAAGA ACTCGATCTA GCTGACAACA GGCTGACAGA GCTCCCTGTC 
CAATTTATGC ACTCCTTCAA GTCTCTCACC TCTCTGAATG TCTCCAGGAA CAACCTGAAG 
AGCTTCCCGG ATCCCTGGTC CTGCCCTTTG AAATGTTGTA AAGCCTCCAA AAATGCCCTG 
GAATCGCTGC CAGACAAAAT GGCTGTCTTT TGGAAAAGCC ACCTTCGGGA TGCAGATTTC 
TCTGAGAACT CTCTGAAAGA GGTGCCCCTG GGATTGTTCC AGCTTGACGC CCTCATGTTC 
TTGAGGTTAC AGGGAAACCA GCTGTTGTCG CTGCCACCTC AAGAGAAGTG GACCTGCACC 
CAACTCAAGA CCCTGGACCT CTCCAGAAAC CAGCTCGGCA AAAATGAAGA TGGGCTTAAA 
ACAAAGAGGA TCTCCCTGTT CACCACCAGA GGGCGCCAGC GTTCTGGAAC TGAGACAGCC 
TCCATGTTGG AATTCCCAGC TTTCCTAAGT GAGTCTTTGG AGGTCCTTTG TCTGAATGAC 
AACCATCTTG ATGCTGTTCC CCCATCGGTC TGCCTGCTGA AGAACCTCTC AGAGCTCTAC 
TTGGGCAATA ACCCTGGCCT CCGAGAGCTC CCTCCGGAGC TAGGCCAGCT GGGGAACCTC 
TGGCAACTGG ACATTGAAGA TCTGAACATT AGCAACGTGC CTGCGGAGGT CAGGAAAGAA 
GGCCCGAAAG CAACACTGTC TTTCCTGCGT GCTCAGCTGC GCAAAGCAGA GAAGTGTAAG 
CTGATGAAGA TGATCCTCGT GGGTCCCCCG CGCCAGGGCA AGTCCACACT CCTGGAGATC 
TTACAGACCG GGAAGGCCCC GCAGCTAGCA CACAGTGAAG CCACCATCAG GACCACCAAG 
TGGGAGCTCC AGAGACCAGC AGGCTCCAAA GCCAAGGTTG AGTCCGTAGA GTTCAACGTC 
TGGGACATCG GGGGTCCGGC CAGCATGGCC ACGGTCAATC AGTGCTTCTT CACAGACAAG 
GCGCTGTATG TGGTGGTCTG GAACCTGGCA CTGGGAGAGG AAGCCGTGGC CAACCTCCAG 
TTCTGGCTGC TCAACATTGA GGCCAAGGCC CCAAATGCCG TTGTGCTGGT GGTCGGGACA 
CACCTGGACC TAATCGAAGC TAAGTTCCGA GTGGAGAGGA TAGCAACCCT GCGTGCCTAC 
GTGCTGGCCC TCTGCCGCTC GCCATCGGGC TCCAGGGCTA CGGGCTTCCC GGACATCACT 
TTCAAACACT TGCATGAGAT CTCCTGCAAG AATCTGGAAG GGCAGGAGGG GCTGCGGCAA 
CTGATCTTCC ACGTCACGTG CAACATGAAG GATGTGGGTA GCACTATCGG CTGTCAAAAA 
CTCGCCGGCA GGCTGATCCC CAGGAGCTAC ATAAGCCTAC AGGAGGCCGT GCTGGCTGAG 
CAACAGCGCC GCAGTCTGGG TGACCAAGTA CAGTATCTGA CGGACAGGCA GCTGGACCAG 
CTGGTAGAGC AGACGCCTGG CAATGATATC AAAGACTATG AAGACCTGCA GTCTGCCATC 
AGCTTCCTCA TAGAAACTGG GACCCTGCTG CACTTTCCAG ACACGAGCCA TGGCCTAAGG 
AACCTCTACT TCCTGGATCC CATCTGGCTC TCTGAATGCC TGCAGAGGAT CTTTAACATC 
AAGGGCTCGC GGTCAGTGGC AAAGAATGGG GTGATCCAAG CGGAAGACCT CAGGATGCTG 
CTGGTGGGGA CAGGCTTCAC ACAGCAGACT GAGGAGCAAT ACTTCCAGTT CCTTGCCAAG 
TTCGAGATTG CCCTCCCTGT TGCCAATGAC AGCTACCTCC TACCACACCT CCTTCCATCC 
AAGCCTGGGC TGGACACCCA CAGCATGCGG CACCCAATGG CTAACACCAT CCAGCGGGTG 
TTTAAGATGA GCTTCGTGCC TGTTGGCTTC TGGCAACGGT TCATAGCACG GATGCTAATC 
AGCTTGGCTG AGATGGACCT GCAGCTTTTT GAAAACAAGA AGAATACGAA AAGCAGGAAC 
CGGAAAGTCA CTATTTACAG TTTTACAGGG AGCCAGAGAA ACCGCTGCAG TACATTCCGA 
GTCCGAAGAA ATCAGACCAT CTACTGGCAG GAAGGGCTGC TGGTCACTTT CGATGGGGGC 
TACCTCAGTG TGGAATCCTC AGATGTGAAC TGGAAAAAGA AAAAAAGCGG AGGAATTAAA 
ATCATCTGCC AGTCAGAGAT GAGGGACTTC TCGGCAATGG CTTTTATCAC AGACCATGTC 
AACTCCCTGA TTGACCAGTG GTTCCCTGCC CTGACGGCCA CAGAAAGTGA CGGGACCCCA 
CTGATGGAGC AGTACGTACC CTGCCCTGTC TGTGAAGCAT CCTGGGCCCA GCATGCAGAT 
CCAAACGAGA GGTCAGAGAG CGTGCAGTAC TTTGATATGG AAGACTGTGT GCTCACAGCC 
ATCGAACGGG ACTTCATCTC CTGCCCCAGA CACCCAGACC TCCCAGTACC ACTTCAGGAG 
CTTGTACCAG AGCTGTTCAT GACTGACTTC CCAGCCAGGC TCTTCCTGGA GAACAGCAAG 
CTGGAGCACA CGGAGGGTGA GAACAGCATT CTGGGCCAAG GTGGCAGTGG CACAGTCATC 
TACCAGGCCC GGTACCAGGG CCAGCCTGTG GCTGTGAAGA GGTTCCATAT CAAGAAGTTC 
AAGAATTCTG CTAATGCCCC AGCAGACACC ATGCTGCGGC ACCTGAGAGC CATGGATGCC 
ATGAAGAACT TCTCAGATTT CCGTCAGGAG GCCAGCATGC TGCATGCCTT GCAGCATCCT 
TGCATCGTGT CACTCATCGG CATCAGCATC CACCCGCTCT GCTTCGCCTT GGAGCTGGCC 
CCGCTGGGCA GTCTCAACAC CGTGCTATCT GAGAATGCCA AAGATTCTTC CTTTATGCCC 
CTGGGACACA TGCTCACCCA AAAGATAGCC TACCAGATTG CCTCGGGCCT GGCCTACCTG 
CACAAGAAAA ACATCATCTT CTGTGACCTC AAGTCGGACA ACATCCTAGT GTGGTCGCTG 
AGCGCCAAGG AGCATATCAA CATCAAACTG TCAGACTACG GCATCTCCAG GCAGTCCTTT 
CACGAGGGGG CCCTGGGTGT GGAGGGCACC CCAGGTTACC AGGCCCCAGA GATCAGGCCT 
CGCATCGTGT ATGACGAGAA GGTAGATATG TTCTCCTACG GCATGGTGCT ATACGAGCTG 
CTGTCAGGAC AGCGTCCTGC ACTGGGCCAC CACCAACTTC AGATTGTCAA GAAGTTGTCC 
AAGGGCATCC GCCCAGTTCT GGGGCAGCCG GAGGAAGTTC AGTTCCATCG GCTGCAGGCG 
CTCATGATGG AGTGCTGGGA CACAAAGCCG GAGAAGCGCC CCCTGGCCTT GTCCGTGGTG 
AGCCAGATGA AAGACCCAAC CTTTGCCACC TTCATGTACA TGCTGCCCTG TGGGAAGCAG 
TCGGCCTTCT TCTCATCCCA GAGCCAGGAG TACACTGTGG TGTTCTGGGA TGGGAAGGAG 
GAGTCCAGGA ATTACACGGT GGTCAACACA GAGAAGGGCC TTCTGGAAGT ACAGAGGATG 
ACCTGCCCCG GGATGAAGTT GAGCTGTCAG CTCAAGGTCC AGAGCTCAGT GTGGATAGCC 
ACGGAGGACC AGAAAATCTA TATCTACAGC CTTAAGGGTA TGTGCCCATT AAGCGTGCCC 
CAGCAGGCCC TGGACACCCC AGCTGTCGTC ACCTGTTTCT TGGCAGTACC TGTTATCAAA 
AAGAACTCCT TCCTGGTGCT GGCAGGCCTG GCTGACGGAC TCGTGGCTGT GTTTCCTGTG 
GCACGGGGCA CCCCAAAAGA AAGCTGCTCC TACCTGTGCT CCCACACAGC CAACAGGTCC 
AAGTTCTGCA TCCCCGACGA AGATGCACGG CAGAACCCTT ACCCAGTGAA GGCAATGGAA 
GTGGTCAACA GTGGGTCTGA GGTCTGGTAC AGCAACGGGC CTGGCCTCCT CGTCATTGAC 
TGCACCATCC TGGACATCAG CAGGCGGCTG GAGCCCTACG CAGCTCCGTC CATGGTCACG 
TCACTTGTGT GCAGCTCAGA CTGCAGAGGA GAGGAGATGG TCTGGTGCCT CGACGACAAG 
GCCAATTGCT TAGTGATGTA CCACTCAGCC ACCTACCAGC TCTGTGCCCG GTACTTCTGT 
GGGGACCCCA ATCCTCTCAG GGACACGTTT TCTGTGCAGC CTTCAGTCCT AGAAACCCCA 
GGCAGTCACA AAACCACCTC AAAGGGGCCT GTGGAAGAGT GCATCGCGGA TGTGAGCATC 
ATGTACAGCG AGGAGCTGGG CACGCAGATC CTCACCCACC AGGACTCGCT CACCGACTAC 
TGCTCCATGT CCTCTTATTC TTCGTCCCCA CCCCACCAAG ATCCTAGGTC CCCTTCCAGT 
CTGCCCAGCT CCCTCACCAG CTATTCCAGT GTGCCTTTCT CTGCCAACTA TGAAGACTCA 
GACAGGCTGC AGGAGCCCAG TGTCACCTCT GACAGAACTG AGCATGACCT GAGCCCCATG 
GATGGGGAGA CGTTCAGCCA GCACCTGCAG GCTGTGAAGG TGCTTGCTGT CAAAGACCTC 
ATTTGGGTTC CTAGACATGG AGGAGATATC ATTGTCATCG GCCTGGAGAA GGATTCAGGT 
GCCCAGCGGG GCAGAGTCAT CGCTGTTTTA AAAGCCCGAG AGCTCAATCG GCACGGGGTT 
CTGGTGGATG CCGCAGTGGT GGCAAAGGAC ACCGTAGTGT GCAGCTTTGC AAATGAAAAC 
ACAGAGTGGT GCCTGGCTGT CTGGAGGGGC TGGGGCGCCA GGGAGTTTGA CATCTTCTAC 
CAGTCCTATG AGGAGCTGGG CCGGCTGGAG GCTTGTACTC GCAAGAGAAG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrk1 ( XM_006540840.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540903.1 Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X4, mRNA.

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CloneID	OMu38812
Clone ID Related Accession (Same CDS sequence)	XM_006540840.3 , XM_006540840.4
Accession Version	XM_006540840.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540840.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGAAT TCCCAGCTTT CCTAAGTGAG TCTTTGGAGG TCCTTTGTCT GAATGACAAC 
CATCTTGATG CTGTTCCCCC ATCGGTCTGC CTGCTGAAGA ACCTCTCAGA GCTCTACTTG 
GGCAATAACC CTGGCCTCCG AGAGCTCCCT CCGGAGCTAG GCCAGCTGGG GAACCTCTGG 
CAACTGGACA TTGAAGATCT GAACATTAGC AACGTGCCTG CGGAGGTCAG GAAAGAAGGC 
CCGAAAGCAA CACTGTCTTT CCTGCGTGCT CAGCTGCGCA AAGCAGAGAA GTGTAAGCTG 
ATGAAGATGA TCCTCGTGGG TCCCCCGCGC CAGGGCAAGT CCACACTCCT GGAGATCTTA 
CAGACCGGGA AGGCCCCGCA GCTAGCACAC AGTGAAGCCA CCATCAGGAC CACCAAGTGG 
GAGCTCCAGA GACCAGCAGG CTCCAAAGCC AAGGTTGAGT CCGTAGAGTT CAACGTCTGG 
GACATCGGGG GTCCGGCCAG CATGGCCACG GTCAATCAGT GCTTCTTCAC AGACAAGGCG 
CTGTATGTGG TGGTCTGGAA CCTGGCACTG GGAGAGGAAG CCGTGGCCAA CCTCCAGTTC 
TGGCTGCTCA ACATTGAGGC CAAGGCCCCA AATGCCGTTG TGCTGGTGGT CGGGACACAC 
CTGGACCTAA TCGAAGCTAA GTTCCGAGTG GAGAGGATAG CAACCCTGCG TGCCTACGTG 
CTGGCCCTCT GCCGCTCGCC ATCGGGCTCC AGGGCTACGG GCTTCCCGGA CATCACTTTC 
AAACACTTGC ATGAGATCTC CTGCAAGAAT CTGGAAGGGC AGGAGGGGCT GCGGCAACTG 
ATCTTCCACG TCACGTGCAA CATGAAGGAT GTGGGTAGCA CTATCGGCTG TCAAAAACTC 
GCCGGCAGGC TGATCCCCAG GAGCTACATA AGCCTACAGG AGGCCGTGCT GGCTGAGCAA 
CAGCGCCGCA GTCTGGGTGA CCAAGTACAG TATCTGACGG ACAGGCAGCT GGACCAGCTG 
GTAGAGCAGA CGCCTGGCAA TGATATCAAA GACTATGAAG ACCTGCAGTC TGCCATCAGC 
TTCCTCATAG AAACTGGGAC CCTGCTGCAC TTTCCAGACA CGAGCCATGG CCTAAGGAAC 
CTCTACTTCC TGGATCCCAT CTGGCTCTCT GAATGCCTGC AGAGGATCTT TAACATCAAG 
GGCTCGCGGT CAGTGGCAAA GAATGGGGTG ATCCAAGCGG AAGACCTCAG GATGCTGCTG 
GTGGGGACAG GCTTCACACA GCAGACTGAG GAGCAATACT TCCAGTTCCT TGCCAAGTTC 
GAGATTGCCC TCCCTGTTGC CAATGACAGC TACCTCCTAC CACACCTCCT TCCATCCAAG 
CCTGGGCTGG ACACCCACAG CATGCGGCAC CCAATGGCTA ACACCATCCA GCGGGTGTTT 
AAGATGAGCT TCGTGCCTGT TGGCTTCTGG CAACGGTTCA TAGCACGGAT GCTAATCAGC 
TTGGCTGAGA TGGACCTGCA GCTTTTTGAA AACAAGAAGA ATACGAAAAG CAGGAACCGG 
AAAGTCACTA TTTACAGTTT TACAGGGAGC CAGAGAAACC GCTGCAGTAC ATTCCGAGTC 
CGAAGAAATC AGACCATCTA CTGGCAGGAA GGGCTGCTGG TCACTTTCGA TGGGGGCTAC 
CTCAGTGTGG AATCCTCAGA TGTGAACTGG AAAAAGAAAA AAAGCGGAGG AATTAAAATC 
ATCTGCCAGT CAGAGATGAG GGACTTCTCG GCAATGGCTT TTATCACAGA CCATGTCAAC 
TCCCTGATTG ACCAGTGGTT CCCTGCCCTG ACGGCCACAG AAAGTGACGG GACCCCACTG 
ATGGAGCAGT ACGTACCCTG CCCTGTCTGT GAAGCATCCT GGGCCCAGCA TGCAGATCCA 
AACGAGAGGT CAGAGAGCGT GCAGTACTTT GATATGGAAG ACTGTGTGCT CACAGCCATC 
GAACGGGACT TCATCTCCTG CCCCAGACAC CCAGACCTCC CAGTACCACT TCAGGAGCTT 
GTACCAGAGC TGTTCATGAC TGACTTCCCA GCCAGGCTCT TCCTGGAGAA CAGCAAGCTG 
GAGCACACGG AGGGTGAGAA CAGCATTCTG GGCCAAGGTG GCAGTGGCAC AGTCATCTAC 
CAGGCCCGGT ACCAGGGCCA GCCTGTGGCT GTGAAGAGGT TCCATATCAA GAAGTTCAAG 
AATTCTGCTA ATGCCCCAGC AGACACCATG CTGCGGCACC TGAGAGCCAT GGATGCCATG 
AAGAACTTCT CAGATTTCCG TCAGGAGGCC AGCATGCTGC ATGCCTTGCA GCATCCTTGC 
ATCGTGTCAC TCATCGGCAT CAGCATCCAC CCGCTCTGCT TCGCCTTGGA GCTGGCCCCG 
CTGGGCAGTC TCAACACCGT GCTATCTGAG AATGCCAAAG ATTCTTCCTT TATGCCCCTG 
GGACACATGC TCACCCAAAA GATAGCCTAC CAGATTGCCT CGGGCCTGGC CTACCTGCAC 
AAGAAAAACA TCATCTTCTG TGACCTCAAG TCGGACAACA TCCTAGTGTG GTCGCTGAGC 
GCCAAGGAGC ATATCAACAT CAAACTGTCA GACTACGGCA TCTCCAGGCA GTCCTTTCAC 
GAGGGGGCCC TGGGTGTGGA GGGCACCCCA GGTTACCAGG CCCCAGAGAT CAGGCCTCGC 
ATCGTGTATG ACGAGAAGGT AGATATGTTC TCCTACGGCA TGGTGCTATA CGAGCTGCTG 
TCAGGACAGC GTCCTGCACT GGGCCACCAC CAACTTCAGA TTGTCAAGAA GTTGTCCAAG 
GGCATCCGCC CAGTTCTGGG GCAGCCGGAG GAAGTTCAGT TCCATCGGCT GCAGGCGCTC 
ATGATGGAGT GCTGGGACAC AAAGCCGGAG AAGCGCCCCC TGGCCTTGTC CGTGGTGAGC 
CAGATGAAAG ACCCAACCTT TGCCACCTTC ATGTACATGC TGCCCTGTGG GAAGCAGTCG 
GCCTTCTTCT CATCCCAGAG CCAGGAGTAC ACTGTGGTGT TCTGGGATGG GAAGGAGGAG 
TCCAGGAATT ACACGGTGGT CAACACAGAG AAGGGCCTTC TGGAAGTACA GAGGATGACC 
TGCCCCGGGA TGAAGTTGAG CTGTCAGCTC AAGGTCCAGA GCTCAGTGTG GATAGCCACG 
GAGGACCAGA AAATCTATAT CTACAGCCTT AAGGGTATGT GCCCATTAAG CGTGCCCCAG 
CAGGCCCTGG ACACCCCAGC TGTCGTCACC TGTTTCTTGG CAGTACCTGT TATCAAAAAG 
AACTCCTTCC TGGTGCTGGC AGGCCTGGCT GACGGACTCG TGGCTGTGTT TCCTGTGGCA 
CGGGGCACCC CAAAAGAAAG CTGCTCCTAC CTGTGCTCCC ACACAGCCAA CAGGTCCAAG 
TTCTGCATCC CCGACGAAGA TGCACGGCAG AACCCTTACC CAGTGAAGGC AATGGAAGTG 
GTCAACAGTG GGTCTGAGGT CTGGTACAGC AACGGGCCTG GCCTCCTCGT CATTGACTGC 
ACCATCCTGG ACATCAGCAG GCGGCTGGAG CCCTACGCAG CTCCGTCCAT GGTCACGTCA 
CTTGTGTGCA GCTCAGACTG CAGAGGAGAG GAGATGGTCT GGTGCCTCGA CGACAAGGCC 
AATTGCTTAG TGATGTACCA CTCAGCCACC TACCAGCTCT GTGCCCGGTA CTTCTGTGGG 
GACCCCAATC CTCTCAGGGA CACGTTTTCT GTGCAGCCTT CAGTCCTAGA AACCCCAGGC 
AGTCACAAAA CCACCTCAAA GGGGCCTGTG GAAGAGTGCA TCGCGGATGT GAGCATCATG 
TACAGCGAGG AGCTGGGCAC GCAGATCCTC ACCCACCAGG ACTCGCTCAC CGACTACTGC 
TCCATGTCCT CTTATTCTTC GTCCCCACCC CACCAAGATC CTAGGTCCCC TTCCAGTCTG 
CCCAGCTCCC TCACCAGCTA TTCCAGTGTG CCTTTCTCTG CCAACTATGA AGACTCAGAC 
AGGCTGCAGG AGCCCAGTGT CACCTCTGAC AGAACTGAGC ATGACCTGAG CCCCATGGAT 
GGGGAGACGT TCAGCCAGCA CCTGCAGGCT GTGAAGGTGC TTGCTGTCAA AGACCTCATT 
TGGGTTCCTA GACATGGAGG AGATATCATT GTCATCGGCC TGGAGAAGGA TTCAGGTGCC 
CAGCGGGGCA GAGTCATCGC TGTTTTAAAA GCCCGAGAGC TCAATCGGCA CGGGGTTCTG 
GTGGATGCCG CAGTGGTGGC AAAGGACACC GTAGTGTGCA GCTTTGCAAA TGAAAACACA 
GAGTGGTGCC TGGCTGTCTG GAGGGGCTGG GGCGCCAGGG AGTTTGACAT CTTCTACCAG 
TCCTATGAGG AGCTGGGCCG GCTGGAGGCT TGTACTCGCA AGAGAAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540903.1
CDS	556..4986
Translation	MLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKNLSELYLGNNPGLRELPPELGQLGNLWQLDIEDLNISNVPAEVRKEGPKATLSFLRAQLRKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQLAHSEATIRTTKWELQRPAGSKAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAGRLIPRSYISLQEAVLAEQQRRSLGDQVQYLTDRQLDQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIQAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQARYQGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLSAKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIVKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSAFFSSQSQEYTVVFWDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWIATEDQKIYIYSLKGMCPLSVPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFPVARGTPKESCSYLCSHTANRSKFCIPDEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCTILDISRRLEPYAAPSMVTSLVCSSDCRGEEMVWCLDDKANCLVMYHSATYQLCARYFCGDPNPLRDTFSVQPSVLETPGSHKTTSKGPVEECIADVSIMYSEELGTQILTHQDSLTDYCSMSSYSSSPPHQDPRSPSSLPSSLTSYSSVPFSANYEDSDRLQEPSVTSDRTEHDLSPMDGETFSQHLQAVKVLAVKDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNRHGVLVDAAVVAKDTVVCSFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR

Target ORF information:

RefSeq Version	XM_006540840.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540840.3

ORF Insert Sequence:

ATGTTGGAAT TCCCAGCTTT CCTAAGTGAG TCTTTGGAGG TCCTTTGTCT GAATGACAAC 
CATCTTGATG CTGTTCCCCC ATCGGTCTGC CTGCTGAAGA ACCTCTCAGA GCTCTACTTG 
GGCAATAACC CTGGCCTCCG AGAGCTCCCT CCGGAGCTAG GCCAGCTGGG GAACCTCTGG 
CAACTGGACA TTGAAGATCT GAACATTAGC AACGTGCCTG CGGAGGTCAG GAAAGAAGGC 
CCGAAAGCAA CACTGTCTTT CCTGCGTGCT CAGCTGCGCA AAGCAGAGAA GTGTAAGCTG 
ATGAAGATGA TCCTCGTGGG TCCCCCGCGC CAGGGCAAGT CCACACTCCT GGAGATCTTA 
CAGACCGGGA AGGCCCCGCA GCTAGCACAC AGTGAAGCCA CCATCAGGAC CACCAAGTGG 
GAGCTCCAGA GACCAGCAGG CTCCAAAGCC AAGGTTGAGT CCGTAGAGTT CAACGTCTGG 
GACATCGGGG GTCCGGCCAG CATGGCCACG GTCAATCAGT GCTTCTTCAC AGACAAGGCG 
CTGTATGTGG TGGTCTGGAA CCTGGCACTG GGAGAGGAAG CCGTGGCCAA CCTCCAGTTC 
TGGCTGCTCA ACATTGAGGC CAAGGCCCCA AATGCCGTTG TGCTGGTGGT CGGGACACAC 
CTGGACCTAA TCGAAGCTAA GTTCCGAGTG GAGAGGATAG CAACCCTGCG TGCCTACGTG 
CTGGCCCTCT GCCGCTCGCC ATCGGGCTCC AGGGCTACGG GCTTCCCGGA CATCACTTTC 
AAACACTTGC ATGAGATCTC CTGCAAGAAT CTGGAAGGGC AGGAGGGGCT GCGGCAACTG 
ATCTTCCACG TCACGTGCAA CATGAAGGAT GTGGGTAGCA CTATCGGCTG TCAAAAACTC 
GCCGGCAGGC TGATCCCCAG GAGCTACATA AGCCTACAGG AGGCCGTGCT GGCTGAGCAA 
CAGCGCCGCA GTCTGGGTGA CCAAGTACAG TATCTGACGG ACAGGCAGCT GGACCAGCTG 
GTAGAGCAGA CGCCTGGCAA TGATATCAAA GACTATGAAG ACCTGCAGTC TGCCATCAGC 
TTCCTCATAG AAACTGGGAC CCTGCTGCAC TTTCCAGACA CGAGCCATGG CCTAAGGAAC 
CTCTACTTCC TGGATCCCAT CTGGCTCTCT GAATGCCTGC AGAGGATCTT TAACATCAAG 
GGCTCGCGGT CAGTGGCAAA GAATGGGGTG ATCCAAGCGG AAGACCTCAG GATGCTGCTG 
GTGGGGACAG GCTTCACACA GCAGACTGAG GAGCAATACT TCCAGTTCCT TGCCAAGTTC 
GAGATTGCCC TCCCTGTTGC CAATGACAGC TACCTCCTAC CACACCTCCT TCCATCCAAG 
CCTGGGCTGG ACACCCACAG CATGCGGCAC CCAATGGCTA ACACCATCCA GCGGGTGTTT 
AAGATGAGCT TCGTGCCTGT TGGCTTCTGG CAACGGTTCA TAGCACGGAT GCTAATCAGC 
TTGGCTGAGA TGGACCTGCA GCTTTTTGAA AACAAGAAGA ATACGAAAAG CAGGAACCGG 
AAAGTCACTA TTTACAGTTT TACAGGGAGC CAGAGAAACC GCTGCAGTAC ATTCCGAGTC 
CGAAGAAATC AGACCATCTA CTGGCAGGAA GGGCTGCTGG TCACTTTCGA TGGGGGCTAC 
CTCAGTGTGG AATCCTCAGA TGTGAACTGG AAAAAGAAAA AAAGCGGAGG AATTAAAATC 
ATCTGCCAGT CAGAGATGAG GGACTTCTCG GCAATGGCTT TTATCACAGA CCATGTCAAC 
TCCCTGATTG ACCAGTGGTT CCCTGCCCTG ACGGCCACAG AAAGTGACGG GACCCCACTG 
ATGGAGCAGT ACGTACCCTG CCCTGTCTGT GAAGCATCCT GGGCCCAGCA TGCAGATCCA 
AACGAGAGGT CAGAGAGCGT GCAGTACTTT GATATGGAAG ACTGTGTGCT CACAGCCATC 
GAACGGGACT TCATCTCCTG CCCCAGACAC CCAGACCTCC CAGTACCACT TCAGGAGCTT 
GTACCAGAGC TGTTCATGAC TGACTTCCCA GCCAGGCTCT TCCTGGAGAA CAGCAAGCTG 
GAGCACACGG AGGGTGAGAA CAGCATTCTG GGCCAAGGTG GCAGTGGCAC AGTCATCTAC 
CAGGCCCGGT ACCAGGGCCA GCCTGTGGCT GTGAAGAGGT TCCATATCAA GAAGTTCAAG 
AATTCTGCTA ATGCCCCAGC AGACACCATG CTGCGGCACC TGAGAGCCAT GGATGCCATG 
AAGAACTTCT CAGATTTCCG TCAGGAGGCC AGCATGCTGC ATGCCTTGCA GCATCCTTGC 
ATCGTGTCAC TCATCGGCAT CAGCATCCAC CCGCTCTGCT TCGCCTTGGA GCTGGCCCCG 
CTGGGCAGTC TCAACACCGT GCTATCTGAG AATGCCAAAG ATTCTTCCTT TATGCCCCTG 
GGACACATGC TCACCCAAAA GATAGCCTAC CAGATTGCCT CGGGCCTGGC CTACCTGCAC 
AAGAAAAACA TCATCTTCTG TGACCTCAAG TCGGACAACA TCCTAGTGTG GTCGCTGAGC 
GCCAAGGAGC ATATCAACAT CAAACTGTCA GACTACGGCA TCTCCAGGCA GTCCTTTCAC 
GAGGGGGCCC TGGGTGTGGA GGGCACCCCA GGTTACCAGG CCCCAGAGAT CAGGCCTCGC 
ATCGTGTATG ACGAGAAGGT AGATATGTTC TCCTACGGCA TGGTGCTATA CGAGCTGCTG 
TCAGGACAGC GTCCTGCACT GGGCCACCAC CAACTTCAGA TTGTCAAGAA GTTGTCCAAG 
GGCATCCGCC CAGTTCTGGG GCAGCCGGAG GAAGTTCAGT TCCATCGGCT GCAGGCGCTC 
ATGATGGAGT GCTGGGACAC AAAGCCGGAG AAGCGCCCCC TGGCCTTGTC CGTGGTGAGC 
CAGATGAAAG ACCCAACCTT TGCCACCTTC ATGTACATGC TGCCCTGTGG GAAGCAGTCG 
GCCTTCTTCT CATCCCAGAG CCAGGAGTAC ACTGTGGTGT TCTGGGATGG GAAGGAGGAG 
TCCAGGAATT ACACGGTGGT CAACACAGAG AAGGGCCTTC TGGAAGTACA GAGGATGACC 
TGCCCCGGGA TGAAGTTGAG CTGTCAGCTC AAGGTCCAGA GCTCAGTGTG GATAGCCACG 
GAGGACCAGA AAATCTATAT CTACAGCCTT AAGGGTATGT GCCCATTAAG CGTGCCCCAG 
CAGGCCCTGG ACACCCCAGC TGTCGTCACC TGTTTCTTGG CAGTACCTGT TATCAAAAAG 
AACTCCTTCC TGGTGCTGGC AGGCCTGGCT GACGGACTCG TGGCTGTGTT TCCTGTGGCA 
CGGGGCACCC CAAAAGAAAG CTGCTCCTAC CTGTGCTCCC ACACAGCCAA CAGGTCCAAG 
TTCTGCATCC CCGACGAAGA TGCACGGCAG AACCCTTACC CAGTGAAGGC AATGGAAGTG 
GTCAACAGTG GGTCTGAGGT CTGGTACAGC AACGGGCCTG GCCTCCTCGT CATTGACTGC 
ACCATCCTGG ACATCAGCAG GCGGCTGGAG CCCTACGCAG CTCCGTCCAT GGTCACGTCA 
CTTGTGTGCA GCTCAGACTG CAGAGGAGAG GAGATGGTCT GGTGCCTCGA CGACAAGGCC 
AATTGCTTAG TGATGTACCA CTCAGCCACC TACCAGCTCT GTGCCCGGTA CTTCTGTGGG 
GACCCCAATC CTCTCAGGGA CACGTTTTCT GTGCAGCCTT CAGTCCTAGA AACCCCAGGC 
AGTCACAAAA CCACCTCAAA GGGGCCTGTG GAAGAGTGCA TCGCGGATGT GAGCATCATG 
TACAGCGAGG AGCTGGGCAC GCAGATCCTC ACCCACCAGG ACTCGCTCAC CGACTACTGC 
TCCATGTCCT CTTATTCTTC GTCCCCACCC CACCAAGATC CTAGGTCCCC TTCCAGTCTG 
CCCAGCTCCC TCACCAGCTA TTCCAGTGTG CCTTTCTCTG CCAACTATGA AGACTCAGAC 
AGGCTGCAGG AGCCCAGTGT CACCTCTGAC AGAACTGAGC ATGACCTGAG CCCCATGGAT 
GGGGAGACGT TCAGCCAGCA CCTGCAGGCT GTGAAGGTGC TTGCTGTCAA AGACCTCATT 
TGGGTTCCTA GACATGGAGG AGATATCATT GTCATCGGCC TGGAGAAGGA TTCAGGTGCC 
CAGCGGGGCA GAGTCATCGC TGTTTTAAAA GCCCGAGAGC TCAATCGGCA CGGGGTTCTG 
GTGGATGCCG CAGTGGTGGC AAAGGACACC GTAGTGTGCA GCTTTGCAAA TGAAAACACA 
GAGTGGTGCC TGGCTGTCTG GAGGGGCTGG GGCGCCAGGG AGTTTGACAT CTTCTACCAG 
TCCTATGAGG AGCTGGGCCG GCTGGAGGCT TGTACTCGCA AGAGAAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrk1 ( NM_146191.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666303.3 Mus musculus leucine-rich repeat kinase 1 (Lrrk1), mRNA.

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CloneID	OMu17582
Clone ID Related Accession (Same CDS sequence)	NM_146191.3
Accession Version	NM_146191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat serine/threonine-protein kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY024941.1, BC072664.1, AK040153.1, AK076432.1 and BC066159.1. On or before Dec 20, 2004 this sequence version replaced XM_485946.1, NM_146191.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147469.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGCA CGTCACAGCG GCCACCCAGT ATGTACTGGT GTGTGGGGAC AGAAGGATTA 
GCTGTGTGTC CAGGACCTGC CATGGAGACC CATAATGGTG CCGAAGACAT GGGCAGCAAA 
CTGTCTTTAC CAGGTGGCAG CTCCACGGTG CAGTGCCCCA GCATGGAAGA AATCCACACT 
GCGTACAAGC AGAGGAACCT CTCCCGAGCC CGAGACCTAC TCAGAGGGGT TTGTGAAGAG 
AGCGAATCCT CACAGGAGAA GGGCCAGCTG CTGAGCATCG CGGCAGCCCA CGGGGATCTG 
GAGACTGTTC AGTTCCTGCT CACTGAGAAG CGCGTGGAGC TGCCGACGGA GCCCACCGAT 
GACAACCCAG CTGTGGTGGC AGCGCATTTT GGACACGCCG AAGTAGTACG TGAACTACTA 
GAGTCCTTGC CAGGTCCCTG TACCCCCCAG CGGCTACTGA ACTGGATGCT GGCCCTGGCT 
TGCCAGCGAG GGCACCTGGA GGTTGTGAAG CTGCTGGTCC TGACACATGG GGCTGACCCG 
GAGAACTATG CCGTCAGGAA GAATGAGTTC CCCGTCATTG TGCGGTTGCC CCTCTATGCG 
GCCATCAAGG CAGGGAATGA AGACATTGCC ATATTCCTGC TTCGGCACGG AGCCTATTTC 
TGCTCCTACA TCCTGCTGGA TAGTCCTGAG CCAAGCAAAC ATCTGCTCAG GAAGTATTTC 
ATCGAAGCCA GCGCCCTGCC CAGCAGCTAT CCCGGGAAAA TCGCACTTCG TGTGAAATGG 
TCCCATCTGA AGTTGCCCTG GGTGGACCTG GACTGGCTCC TAGACATCTC CTGCCAGATC 
ACAGAGCTTG ACCTGTCTGC CAACTGCCTG CCTTCGCTCC CTTCCATCAT CCCTTGGGGA 
CTCATCAATC TAAAGAAGCT GAATCTCTCA AACAACCAGC TGGGGGAGCT GCCCTGCGTG 
CAGTCCTCCG ACGAAATCAT CTGCTCCAGG TTACTTGAAA TTGACATCTC CAGCAACAAA 
CTGTCTCACC TCCCTCCTGG ATTCCTGCAC CTCTCAAAAC TTCAAAAGCT GACTGCTTCC 
AAAAACTACC TGGAGCGGCT GTTTGAAGAA GAAAACGCCA CTAACTGGAT CGGCCTGCGG 
AAGCTGCAAG AACTCGATCT AGCTGACAAC AGGCTGACAG AGCTCCCTGT CCAATTTATG 
CACTCCTTCA AGTCTCTCAC CTCTCTGAAT GTCTCCAGGA ACAACCTGAA GAGCTTCCCG 
GATCCCTGGT CCTGCCCTTT GAAATGTTGT AAAGCCTCCA AAAATGCCCT GGAATCGCTG 
CCAGACAAAA TGGCTGTCTT TTGGAAAAGC CACCTTCGGG ATGCAGATTT CTCTGAGAAC 
TCTCTGAAAG AGGTGCCCCT GGGATTGTTC CAGCTTGACG CCCTCATGTT CTTGAGGTTA 
CAGGGAAACC AGCTGTTGTC GCTGCCACCT CAAGAGAAGT GGACCTGCAC CCAACTCAAG 
ACCCTGGACC TCTCCAGAAA CCAGCTCGGC AAAAATGAAG ATGGGCTTAA AACAAAGAGG 
ATCTCCCTGT TCACCACCAG AGGGCGCCAG CGTTCTGGAA CTGAGACAGC CTCCATGTTG 
GAATTCCCAG CTTTCCTAAG TGAGTCTTTG GAGGTCCTTT GTCTGAATGA CAACCATCTT 
GATGCTGTTC CCCCATCGGT CTGCCTGCTG AAGAACCTCT CAGAGCTCTA CTTGGGCAAT 
AACCCTGGCC TCCGAGAGCT CCCTCCGGAG CTAGGCCAGC TGGGGAACCT CTGGCAACTG 
GACATTGAAG ATCTGAACAT TAGCAACGTG CCTGCGGAGG TCAGGAAAGA AGGCCCGAAA 
GCAACACTGT CTTTCCTGCG TGCTCAGCTG CGCAAAGCAG AGAAGTGTAA GCTGATGAAG 
ATGATCCTCG TGGGTCCCCC GCGCCAGGGC AAGTCCACAC TCCTGGAGAT CTTACAGACC 
GGGAAGGCCC CGCAGCTAGC ACACAGTGAA GCCACCATCA GGACCACCAA GTGGGAGCTC 
CAGAGACCAG CAGGCTCCAA AGCCAAGGTT GAGTCCGTAG AGTTCAACGT CTGGGACATC 
GGGGGTCCGG CCAGCATGGC CACGGTCAAT CAGTGCTTCT TCACAGACAA GGCGCTGTAT 
GTGGTGGTCT GGAACCTGGC ACTGGGAGAG GAAGCCGTGG CCAACCTCCA GTTCTGGCTG 
CTCAACATTG AGGCCAAGGC CCCAAATGCC GTTGTGCTGG TGGTCGGGAC ACACCTGGAC 
CTAATCGAAG CTAAGTTCCG AGTGGAGAGG ATAGCAACCC TGCGTGCCTA CGTGCTGGCC 
CTCTGCCGCT CGCCATCGGG CTCCAGGGCT ACGGGCTTCC CGGACATCAC TTTCAAACAC 
TTGCATGAGA TCTCCTGCAA GAATCTGGAA GGGCAGGAGG GGCTGCGGCA ACTGATCTTC 
CACGTCACGT GCAACATGAA GGATGTGGGT AGCACTATCG GCTGTCAAAA ACTCGCCGGC 
AGGCTGATCC CCAGGAGCTA CATAAGCCTA CAGGAGGCCG TGCTGGCTGA GCAACAGCGC 
CGCAGTCTGG GTGACCAAGT ACAGTATCTG ACGGACAGGC AGCTGGACCA GCTGGTAGAG 
CAGACGCCTG GCAATGATAT CAAAGACTAT GAAGACCTGC AGTCTGCCAT CAGCTTCCTC 
ATAGAAACTG GGACCCTGCT GCACTTTCCA GACACGAGCC ATGGCCTAAG GAACCTCTAC 
TTCCTGGATC CCATCTGGCT CTCTGAATGC CTGCAGAGGA TCTTTAACAT CAAGGGCTCG 
CGGTCAGTGG CAAAGAATGG GGTGATCCAA GCGGAAGACC TCAGGATGCT GCTGGTGGGG 
ACAGGCTTCA CACAGCAGAC TGAGGAGCAA TACTTCCAGT TCCTTGCCAA GTTCGAGATT 
GCCCTCCCTG TTGCCAATGA CAGCTACCTC CTACCACACC TCCTTCCATC CAAGCCTGGG 
CTGGACACCC ACAGCATGCG GCACCCAATG GCTAACACCA TCCAGCGGGT GTTTAAGATG 
AGCTTCGTGC CTGTTGGCTT CTGGCAACGG TTCATAGCAC GGATGCTAAT CAGCTTGGCT 
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACGA AAAGCAGGAA CCGGAAAGTC 
ACTATTTACA GTTTTACAGG GAGCCAGAGA AACCGCTGCA GTACATTCCG AGTCCGAAGA 
AATCAGACCA TCTACTGGCA GGAAGGGCTG CTGGTCACTT TCGATGGGGG CTACCTCAGT 
GTGGAATCCT CAGATGTGAA CTGGAAAAAG AAAAAAAGCG GAGGAATTAA AATCATCTGC 
CAGTCAGAGA TGAGGGACTT CTCGGCAATG GCTTTTATCA CAGACCATGT CAACTCCCTG 
ATTGACCAGT GGTTCCCTGC CCTGACGGCC ACAGAAAGTG ACGGGACCCC ACTGATGGAG 
CAGTACGTAC CCTGCCCTGT CTGTGAAGCA TCCTGGGCCC AGCATGCAGA TCCAAACGAG 
AGGTCAGAGA GCGTGCAGTA CTTTGATATG GAAGACTGTG TGCTCACAGC CATCGAACGG 
GACTTCATCT CCTGCCCCAG ACACCCAGAC CTCCCAGTAC CACTTCAGGA GCTTGTACCA 
GAGCTGTTCA TGACTGACTT CCCAGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC 
ACGGAGGGTG AGAACAGCAT TCTGGGCCAA GGTGGCAGTG GCACAGTCAT CTACCAGGCC 
CGGTACCAGG GCCAGCCTGT GGCTGTGAAG AGGTTCCATA TCAAGAAGTT CAAGAATTCT 
GCTAATGCCC CAGCAGACAC CATGCTGCGG CACCTGAGAG CCATGGATGC CATGAAGAAC 
TTCTCAGATT TCCGTCAGGA GGCCAGCATG CTGCATGCCT TGCAGCATCC TTGCATCGTG 
TCACTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCT TGGAGCTGGC CCCGCTGGGC 
AGTCTCAACA CCGTGCTATC TGAGAATGCC AAAGATTCTT CCTTTATGCC CCTGGGACAC 
ATGCTCACCC AAAAGATAGC CTACCAGATT GCCTCGGGCC TGGCCTACCT GCACAAGAAA 
AACATCATCT TCTGTGACCT CAAGTCGGAC AACATCCTAG TGTGGTCGCT GAGCGCCAAG 
GAGCATATCA ACATCAAACT GTCAGACTAC GGCATCTCCA GGCAGTCCTT TCACGAGGGG 
GCCCTGGGTG TGGAGGGCAC CCCAGGTTAC CAGGCCCCAG AGATCAGGCC TCGCATCGTG 
TATGACGAGA AGGTAGATAT GTTCTCCTAC GGCATGGTGC TATACGAGCT GCTGTCAGGA 
CAGCGTCCTG CACTGGGCCA CCACCAACTT CAGATTGTCA AGAAGTTGTC CAAGGGCATC 
CGCCCAGTTC TGGGGCAGCC GGAGGAAGTT CAGTTCCATC GGCTGCAGGC GCTCATGATG 
GAGTGCTGGG ACACAAAGCC GGAGAAGCGC CCCCTGGCCT TGTCCGTGGT GAGCCAGATG 
AAAGACCCAA CCTTTGCCAC CTTCATGTAC ATGCTGCCCT GTGGGAAGCA GTCGGCCTTC 
TTCTCATCCC AGAGCCAGGA GTACACTGTG GTGTTCTGGG ATGGGAAGGA GGAGTCCAGG 
AATTACACGG TGGTCAACAC AGAGAAGGGC CTTCTGGAAG TACAGAGGAT GACCTGCCCC 
GGGATGAAGT TGAGCTGTCA GCTCAAGGTC CAGAGCTCAG TGTGGATAGC CACGGAGGAC 
CAGAAAATCT ATATCTACAG CCTTAAGGGT ATGTGCCCAT TAAGCGTGCC CCAGCAGGCC 
CTGGACACCC CAGCTGTCGT CACCTGTTTC TTGGCAGTAC CTGTTATCAA AAAGAACTCC 
TTCCTGGTGC TGGCAGGCCT GGCTGACGGA CTCGTGGCTG TGTTTCCTGT GGCACGGGGC 
ACCCCAAAAG AAAGCTGCTC CTACCTGTGC TCCCACACAG CCAACAGGTC CAAGTTCTGC 
ATCCCCGACG AAGATGCACG GCAGAACCCT TACCCAGTGA AGGCAATGGA AGTGGTCAAC 
AGTGGGTCTG AGGTCTGGTA CAGCAACGGG CCTGGCCTCC TCGTCATTGA CTGCACCATC 
CTGGACATCA GCAGGCGGCT GGAGCCCTAC GCAGCTCCGT CCATGGTCAC GTCACTTGTG 
TGCAGCTCAG ACTGCAGAGG AGAGGAGATG GTCTGGTGCC TCGACGACAA GGCCAATTGC 
TTAGTGATGT ACCACTCAGC CACCTACCAG CTCTGTGCCC GGTACTTCTG TGGGGACCCC 
AATCCTCTCA GGGACACGTT TTCTGTGCAG CCTTCAGTCC TAGAAACCCC AGGCAGTCAC 
AAAACCACCT CAAAGGGGCC TGTGGAAGAG TGCATCGCGG ATGTGAGCAT CATGTACAGC 
GAGGAGCTGG GCACGCAGAT CCTCACCCAC CAGGACTCGC TCACCGACTA CTGCTCCATG 
TCCTCTTATT CTTCGTCCCC ACCCCACCAA GATCCTAGGT CCCCTTCCAG TCTGCCCAGC 
TCCCTCACCA GCTATTCCAG TGTGCCTTTC TCTGCCAACT ATGAAGACTC AGACAGGCTG 
CAGGAGCCCA GTGTCACCTC TGACAGAACT GAGCATGACC TGAGCCCCAT GGATGGGGAG 
ACGTTCAGCC AGCACCTGCA GGCTGTGAAG GTGCTTGCTG TCAAAGACCT CATTTGGGTT 
CCTAGACATG GAGGAGATAT CATTGTCATC GGCCTGGAGA AGGATTCAGG TGCCCAGCGG 
GGCAGAGTCA TCGCTGTTTT AAAAGCCCGA GAGCTCAATC GGCACGGGGT TCTGGTGGAT 
GCCGCAGTGG TGGCAAAGGA CACCGTAGTG TGCAGCTTTG CAAATGAAAA CACAGAGTGG 
TGCCTGGCTG TCTGGAGGGG CTGGGGCGCC AGGGAGTTTG ACATCTTCTA CCAGTCCTAT 
GAGGAGCTGG GCCGGCTGGA GGCTTGTACT CGCAAGAGAA GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666303.3
CDS	241..6285
Translation	MAGTSQRPPSMYWCVGTEGLAVCPGPAMETHNGAEDMGSKLSLPGGSSTVQCPSMEEIHTAYKQRNLSRARDLLRGVCEESESSQEKGQLLSIAAAHGDLETVQFLLTEKRVELPTEPTDDNPAVVAAHFGHAEVVRELLESLPGPCTPQRLLNWMLALACQRGHLEVVKLLVLTHGADPENYAVRKNEFPVIVRLPLYAAIKAGNEDIAIFLLRHGAYFCSYILLDSPEPSKHLLRKYFIEASALPSSYPGKIALRVKWSHLKLPWVDLDWLLDISCQITELDLSANCLPSLPSIIPWGLINLKKLNLSNNQLGELPCVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLERLFEEENATNWIGLRKLQELDLADNRLTELPVQFMHSFKSLTSLNVSRNNLKSFPDPWSCPLKCCKASKNALESLPDKMAVFWKSHLRDADFSENSLKEVPLGLFQLDALMFLRLQGNQLLSLPPQEKWTCTQLKTLDLSRNQLGKNEDGLKTKRISLFTTRGRQRSGTETASMLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKNLSELYLGNNPGLRELPPELGQLGNLWQLDIEDLNISNVPAEVRKEGPKATLSFLRAQLRKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQLAHSEATIRTTKWELQRPAGSKAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAGRLIPRSYISLQEAVLAEQQRRSLGDQVQYLTDRQLDQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIQAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQARYQGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLSAKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIVKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSAFFSSQSQEYTVVFWDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWIATEDQKIYIYSLKGMCPLSVPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFPVARGTPKESCSYLCSHTANRSKFCIPDEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCTILDISRRLEPYAAPSMVTSLVCSSDCRGEEMVWCLDDKANCLVMYHSATYQLCARYFCGDPNPLRDTFSVQPSVLETPGSHKTTSKGPVEECIADVSIMYSEELGTQILTHQDSLTDYCSMSSYSSSPPHQDPRSPSSLPSSLTSYSSVPFSANYEDSDRLQEPSVTSDRTEHDLSPMDGETFSQHLQAVKVLAVKDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNRHGVLVDAAVVAKDTVVCSFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR

Target ORF information:

RefSeq Version	NM_146191.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146191.3

ORF Insert Sequence:

ATGGCTGGCA CGTCACAGCG GCCACCCAGT ATGTACTGGT GTGTGGGGAC AGAAGGATTA 
GCTGTGTGTC CAGGACCTGC CATGGAGACC CATAATGGTG CCGAAGACAT GGGCAGCAAA 
CTGTCTTTAC CAGGTGGCAG CTCCACGGTG CAGTGCCCCA GCATGGAAGA AATCCACACT 
GCGTACAAGC AGAGGAACCT CTCCCGAGCC CGAGACCTAC TCAGAGGGGT TTGTGAAGAG 
AGCGAATCCT CACAGGAGAA GGGCCAGCTG CTGAGCATCG CGGCAGCCCA CGGGGATCTG 
GAGACTGTTC AGTTCCTGCT CACTGAGAAG CGCGTGGAGC TGCCGACGGA GCCCACCGAT 
GACAACCCAG CTGTGGTGGC AGCGCATTTT GGACACGCCG AAGTAGTACG TGAACTACTA 
GAGTCCTTGC CAGGTCCCTG TACCCCCCAG CGGCTACTGA ACTGGATGCT GGCCCTGGCT 
TGCCAGCGAG GGCACCTGGA GGTTGTGAAG CTGCTGGTCC TGACACATGG GGCTGACCCG 
GAGAACTATG CCGTCAGGAA GAATGAGTTC CCCGTCATTG TGCGGTTGCC CCTCTATGCG 
GCCATCAAGG CAGGGAATGA AGACATTGCC ATATTCCTGC TTCGGCACGG AGCCTATTTC 
TGCTCCTACA TCCTGCTGGA TAGTCCTGAG CCAAGCAAAC ATCTGCTCAG GAAGTATTTC 
ATCGAAGCCA GCGCCCTGCC CAGCAGCTAT CCCGGGAAAA TCGCACTTCG TGTGAAATGG 
TCCCATCTGA AGTTGCCCTG GGTGGACCTG GACTGGCTCC TAGACATCTC CTGCCAGATC 
ACAGAGCTTG ACCTGTCTGC CAACTGCCTG CCTTCGCTCC CTTCCATCAT CCCTTGGGGA 
CTCATCAATC TAAAGAAGCT GAATCTCTCA AACAACCAGC TGGGGGAGCT GCCCTGCGTG 
CAGTCCTCCG ACGAAATCAT CTGCTCCAGG TTACTTGAAA TTGACATCTC CAGCAACAAA 
CTGTCTCACC TCCCTCCTGG ATTCCTGCAC CTCTCAAAAC TTCAAAAGCT GACTGCTTCC 
AAAAACTACC TGGAGCGGCT GTTTGAAGAA GAAAACGCCA CTAACTGGAT CGGCCTGCGG 
AAGCTGCAAG AACTCGATCT AGCTGACAAC AGGCTGACAG AGCTCCCTGT CCAATTTATG 
CACTCCTTCA AGTCTCTCAC CTCTCTGAAT GTCTCCAGGA ACAACCTGAA GAGCTTCCCG 
GATCCCTGGT CCTGCCCTTT GAAATGTTGT AAAGCCTCCA AAAATGCCCT GGAATCGCTG 
CCAGACAAAA TGGCTGTCTT TTGGAAAAGC CACCTTCGGG ATGCAGATTT CTCTGAGAAC 
TCTCTGAAAG AGGTGCCCCT GGGATTGTTC CAGCTTGACG CCCTCATGTT CTTGAGGTTA 
CAGGGAAACC AGCTGTTGTC GCTGCCACCT CAAGAGAAGT GGACCTGCAC CCAACTCAAG 
ACCCTGGACC TCTCCAGAAA CCAGCTCGGC AAAAATGAAG ATGGGCTTAA AACAAAGAGG 
ATCTCCCTGT TCACCACCAG AGGGCGCCAG CGTTCTGGAA CTGAGACAGC CTCCATGTTG 
GAATTCCCAG CTTTCCTAAG TGAGTCTTTG GAGGTCCTTT GTCTGAATGA CAACCATCTT 
GATGCTGTTC CCCCATCGGT CTGCCTGCTG AAGAACCTCT CAGAGCTCTA CTTGGGCAAT 
AACCCTGGCC TCCGAGAGCT CCCTCCGGAG CTAGGCCAGC TGGGGAACCT CTGGCAACTG 
GACATTGAAG ATCTGAACAT TAGCAACGTG CCTGCGGAGG TCAGGAAAGA AGGCCCGAAA 
GCAACACTGT CTTTCCTGCG TGCTCAGCTG CGCAAAGCAG AGAAGTGTAA GCTGATGAAG 
ATGATCCTCG TGGGTCCCCC GCGCCAGGGC AAGTCCACAC TCCTGGAGAT CTTACAGACC 
GGGAAGGCCC CGCAGCTAGC ACACAGTGAA GCCACCATCA GGACCACCAA GTGGGAGCTC 
CAGAGACCAG CAGGCTCCAA AGCCAAGGTT GAGTCCGTAG AGTTCAACGT CTGGGACATC 
GGGGGTCCGG CCAGCATGGC CACGGTCAAT CAGTGCTTCT TCACAGACAA GGCGCTGTAT 
GTGGTGGTCT GGAACCTGGC ACTGGGAGAG GAAGCCGTGG CCAACCTCCA GTTCTGGCTG 
CTCAACATTG AGGCCAAGGC CCCAAATGCC GTTGTGCTGG TGGTCGGGAC ACACCTGGAC 
CTAATCGAAG CTAAGTTCCG AGTGGAGAGG ATAGCAACCC TGCGTGCCTA CGTGCTGGCC 
CTCTGCCGCT CGCCATCGGG CTCCAGGGCT ACGGGCTTCC CGGACATCAC TTTCAAACAC 
TTGCATGAGA TCTCCTGCAA GAATCTGGAA GGGCAGGAGG GGCTGCGGCA ACTGATCTTC 
CACGTCACGT GCAACATGAA GGATGTGGGT AGCACTATCG GCTGTCAAAA ACTCGCCGGC 
AGGCTGATCC CCAGGAGCTA CATAAGCCTA CAGGAGGCCG TGCTGGCTGA GCAACAGCGC 
CGCAGTCTGG GTGACCAAGT ACAGTATCTG ACGGACAGGC AGCTGGACCA GCTGGTAGAG 
CAGACGCCTG GCAATGATAT CAAAGACTAT GAAGACCTGC AGTCTGCCAT CAGCTTCCTC 
ATAGAAACTG GGACCCTGCT GCACTTTCCA GACACGAGCC ATGGCCTAAG GAACCTCTAC 
TTCCTGGATC CCATCTGGCT CTCTGAATGC CTGCAGAGGA TCTTTAACAT CAAGGGCTCG 
CGGTCAGTGG CAAAGAATGG GGTGATCCAA GCGGAAGACC TCAGGATGCT GCTGGTGGGG 
ACAGGCTTCA CACAGCAGAC TGAGGAGCAA TACTTCCAGT TCCTTGCCAA GTTCGAGATT 
GCCCTCCCTG TTGCCAATGA CAGCTACCTC CTACCACACC TCCTTCCATC CAAGCCTGGG 
CTGGACACCC ACAGCATGCG GCACCCAATG GCTAACACCA TCCAGCGGGT GTTTAAGATG 
AGCTTCGTGC CTGTTGGCTT CTGGCAACGG TTCATAGCAC GGATGCTAAT CAGCTTGGCT 
GAGATGGACC TGCAGCTTTT TGAAAACAAG AAGAATACGA AAAGCAGGAA CCGGAAAGTC 
ACTATTTACA GTTTTACAGG GAGCCAGAGA AACCGCTGCA GTACATTCCG AGTCCGAAGA 
AATCAGACCA TCTACTGGCA GGAAGGGCTG CTGGTCACTT TCGATGGGGG CTACCTCAGT 
GTGGAATCCT CAGATGTGAA CTGGAAAAAG AAAAAAAGCG GAGGAATTAA AATCATCTGC 
CAGTCAGAGA TGAGGGACTT CTCGGCAATG GCTTTTATCA CAGACCATGT CAACTCCCTG 
ATTGACCAGT GGTTCCCTGC CCTGACGGCC ACAGAAAGTG ACGGGACCCC ACTGATGGAG 
CAGTACGTAC CCTGCCCTGT CTGTGAAGCA TCCTGGGCCC AGCATGCAGA TCCAAACGAG 
AGGTCAGAGA GCGTGCAGTA CTTTGATATG GAAGACTGTG TGCTCACAGC CATCGAACGG 
GACTTCATCT CCTGCCCCAG ACACCCAGAC CTCCCAGTAC CACTTCAGGA GCTTGTACCA 
GAGCTGTTCA TGACTGACTT CCCAGCCAGG CTCTTCCTGG AGAACAGCAA GCTGGAGCAC 
ACGGAGGGTG AGAACAGCAT TCTGGGCCAA GGTGGCAGTG GCACAGTCAT CTACCAGGCC 
CGGTACCAGG GCCAGCCTGT GGCTGTGAAG AGGTTCCATA TCAAGAAGTT CAAGAATTCT 
GCTAATGCCC CAGCAGACAC CATGCTGCGG CACCTGAGAG CCATGGATGC CATGAAGAAC 
TTCTCAGATT TCCGTCAGGA GGCCAGCATG CTGCATGCCT TGCAGCATCC TTGCATCGTG 
TCACTCATCG GCATCAGCAT CCACCCGCTC TGCTTCGCCT TGGAGCTGGC CCCGCTGGGC 
AGTCTCAACA CCGTGCTATC TGAGAATGCC AAAGATTCTT CCTTTATGCC CCTGGGACAC 
ATGCTCACCC AAAAGATAGC CTACCAGATT GCCTCGGGCC TGGCCTACCT GCACAAGAAA 
AACATCATCT TCTGTGACCT CAAGTCGGAC AACATCCTAG TGTGGTCGCT GAGCGCCAAG 
GAGCATATCA ACATCAAACT GTCAGACTAC GGCATCTCCA GGCAGTCCTT TCACGAGGGG 
GCCCTGGGTG TGGAGGGCAC CCCAGGTTAC CAGGCCCCAG AGATCAGGCC TCGCATCGTG 
TATGACGAGA AGGTAGATAT GTTCTCCTAC GGCATGGTGC TATACGAGCT GCTGTCAGGA 
CAGCGTCCTG CACTGGGCCA CCACCAACTT CAGATTGTCA AGAAGTTGTC CAAGGGCATC 
CGCCCAGTTC TGGGGCAGCC GGAGGAAGTT CAGTTCCATC GGCTGCAGGC GCTCATGATG 
GAGTGCTGGG ACACAAAGCC GGAGAAGCGC CCCCTGGCCT TGTCCGTGGT GAGCCAGATG 
AAAGACCCAA CCTTTGCCAC CTTCATGTAC ATGCTGCCCT GTGGGAAGCA GTCGGCCTTC 
TTCTCATCCC AGAGCCAGGA GTACACTGTG GTGTTCTGGG ATGGGAAGGA GGAGTCCAGG 
AATTACACGG TGGTCAACAC AGAGAAGGGC CTTCTGGAAG TACAGAGGAT GACCTGCCCC 
GGGATGAAGT TGAGCTGTCA GCTCAAGGTC CAGAGCTCAG TGTGGATAGC CACGGAGGAC 
CAGAAAATCT ATATCTACAG CCTTAAGGGT ATGTGCCCAT TAAGCGTGCC CCAGCAGGCC 
CTGGACACCC CAGCTGTCGT CACCTGTTTC TTGGCAGTAC CTGTTATCAA AAAGAACTCC 
TTCCTGGTGC TGGCAGGCCT GGCTGACGGA CTCGTGGCTG TGTTTCCTGT GGCACGGGGC 
ACCCCAAAAG AAAGCTGCTC CTACCTGTGC TCCCACACAG CCAACAGGTC CAAGTTCTGC 
ATCCCCGACG AAGATGCACG GCAGAACCCT TACCCAGTGA AGGCAATGGA AGTGGTCAAC 
AGTGGGTCTG AGGTCTGGTA CAGCAACGGG CCTGGCCTCC TCGTCATTGA CTGCACCATC 
CTGGACATCA GCAGGCGGCT GGAGCCCTAC GCAGCTCCGT CCATGGTCAC GTCACTTGTG 
TGCAGCTCAG ACTGCAGAGG AGAGGAGATG GTCTGGTGCC TCGACGACAA GGCCAATTGC 
TTAGTGATGT ACCACTCAGC CACCTACCAG CTCTGTGCCC GGTACTTCTG TGGGGACCCC 
AATCCTCTCA GGGACACGTT TTCTGTGCAG CCTTCAGTCC TAGAAACCCC AGGCAGTCAC 
AAAACCACCT CAAAGGGGCC TGTGGAAGAG TGCATCGCGG ATGTGAGCAT CATGTACAGC 
GAGGAGCTGG GCACGCAGAT CCTCACCCAC CAGGACTCGC TCACCGACTA CTGCTCCATG 
TCCTCTTATT CTTCGTCCCC ACCCCACCAA GATCCTAGGT CCCCTTCCAG TCTGCCCAGC 
TCCCTCACCA GCTATTCCAG TGTGCCTTTC TCTGCCAACT ATGAAGACTC AGACAGGCTG 
CAGGAGCCCA GTGTCACCTC TGACAGAACT GAGCATGACC TGAGCCCCAT GGATGGGGAG 
ACGTTCAGCC AGCACCTGCA GGCTGTGAAG GTGCTTGCTG TCAAAGACCT CATTTGGGTT 
CCTAGACATG GAGGAGATAT CATTGTCATC GGCCTGGAGA AGGATTCAGG TGCCCAGCGG 
GGCAGAGTCA TCGCTGTTTT AAAAGCCCGA GAGCTCAATC GGCACGGGGT TCTGGTGGAT 
GCCGCAGTGG TGGCAAAGGA CACCGTAGTG TGCAGCTTTG CAAATGAAAA CACAGAGTGG 
TGCCTGGCTG TCTGGAGGGG CTGGGGCGCC AGGGAGTTTG ACATCTTCTA CCAGTCCTAT 
GAGGAGCTGG GCCGGCTGGA GGCTTGTACT CGCAAGAGAA GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lrrk1 ( XM_006540838.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006540901.1 Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X1, mRNA.

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CloneID	OMu38810
Clone ID Related Accession (Same CDS sequence)	XM_006540838.3
Accession Version	XM_006540838.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	leucine-rich repeat serine/threonine-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006540838.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCCA GAATGGCTGG GGGTCCCCAG AGATATAGGA GCCTGCTGAC AGGAGCTTTT 
CAGGATTCTC TTCCTAACCC CCAGCCCTGT GATGTCATGG GTGCCGAAGA CATGGGCAGC 
AAACTGTCTT TACCAGGTGG CAGCTCCACG GTGCAGTGCC CCAGCATGGA AGAAATCCAC 
ACTGCGTACA AGCAGAGGAA CCTCTCCCGA GCCCGAGACC TACTCAGAGG GGTTTGTGAA 
GAGAGCGAAT CCTCACAGGA GAAGGGCCAG CTGCTGAGCA TCGCGGCAGC CCACGGGGAT 
CTGGAGACTG TTCAGTTCCT GCTCACTGAG AAGCGCGTGG AGCTGCCGAC GGAGCCCACC 
GATGACAACC CAGCTGTGGT GGCAGCGCAT TTTGGACACG CCGAAGTAGT ACGTGAACTA 
CTAGAGTCCT TGCCAGGTCC CTGTACCCCC CAGCGGCTAC TGAACTGGAT GCTGGCCCTG 
GCTTGCCAGC GAGGGCACCT GGAGGTTGTG AAGCTGCTGG TCCTGACACA TGGGGCTGAC 
CCGGAGAACT ATGCCGTCAG GAAGAATGAG TTCCCCGTCA TTGTGCGGTT GCCCCTCTAT 
GCGGCCATCA AGGCAGGGAA TGAAGACATT GCCATATTCC TGCTTCGGCA CGGAGCCTAT 
TTCTGCTCCT ACATCCTGCT GGATAGTCCT GAGCCAAGCA AACATCTGCT CAGGAAGTAT 
TTCATCGAAG CCAGCGCCCT GCCCAGCAGC TATCCCGGGA AAATCGCACT TCGTGTGAAA 
TGGTCCCATC TGAAGTTGCC CTGGGTGGAC CTGGACTGGC TCCTAGACAT CTCCTGCCAG 
ATCACAGAGC TTGACCTGTC TGCCAACTGC CTGCCTTCGC TCCCTTCCAT CATCCCTTGG 
GGACTCATCA ATCTAAAGAA GCTGAATCTC TCAAACAACC AGCTGGGGGA GCTGCCCTGC 
GTGCAGTCCT CCGACGAAAT CATCTGCTCC AGGTTACTTG AAATTGACAT CTCCAGCAAC 
AAACTGTCTC ACCTCCCTCC TGGATTCCTG CACCTCTCAA AACTTCAAAA GCTGACTGCT 
TCCAAAAACT ACCTGGAGCG GCTGTTTGAA GAAGAAAACG CCACTAACTG GATCGGCCTG 
CGGAAGCTGC AAGAACTCGA TCTAGCTGAC AACAGGCTGA CAGAGCTCCC TGTCCAATTT 
ATGCACTCCT TCAAGTCTCT CACCTCTCTG AATGTCTCCA GGAACAACCT GAAGAGCTTC 
CCGGATCCCT GGTCCTGCCC TTTGAAATGT TGTAAAGCCT CCAAAAATGC CCTGGAATCG 
CTGCCAGACA AAATGGCTGT CTTTTGGAAA AGCCACCTTC GGGATGCAGA TTTCTCTGAG 
AACTCTCTGA AAGAGGTGCC CCTGGGATTG TTCCAGCTTG ACGCCCTCAT GTTCTTGAGG 
TTACAGGGAA ACCAGCTGTT GTCGCTGCCA CCTCAAGAGA AGTGGACCTG CACCCAACTC 
AAGACCCTGG ACCTCTCCAG AAACCAGCTC GGCAAAAATG AAGATGGGCT TAAAACAAAG 
AGGATCTCCC TGTTCACCAC CAGAGGGCGC CAGCGTTCTG GAACTGAGAC AGCCTCCATG 
TTGGAATTCC CAGCTTTCCT AAGTGAGTCT TTGGAGGTCC TTTGTCTGAA TGACAACCAT 
CTTGATGCTG TTCCCCCATC GGTCTGCCTG CTGAAGAACC TCTCAGAGCT CTACTTGGGC 
AATAACCCTG GCCTCCGAGA GCTCCCTCCG GAGCTAGGCC AGCTGGGGAA CCTCTGGCAA 
CTGGACATTG AAGATCTGAA CATTAGCAAC GTGCCTGCGG AGGTCAGGAA AGAAGGCCCG 
AAAGCAACAC TGTCTTTCCT GCGTGCTCAG CTGCGCAAAG CAGAGAAGTG TAAGCTGATG 
AAGATGATCC TCGTGGGTCC CCCGCGCCAG GGCAAGTCCA CACTCCTGGA GATCTTACAG 
ACCGGGAAGG CCCCGCAGCT AGCACACAGT GAAGCCACCA TCAGGACCAC CAAGTGGGAG 
CTCCAGAGAC CAGCAGGCTC CAAAGCCAAG GTTGAGTCCG TAGAGTTCAA CGTCTGGGAC 
ATCGGGGGTC CGGCCAGCAT GGCCACGGTC AATCAGTGCT TCTTCACAGA CAAGGCGCTG 
TATGTGGTGG TCTGGAACCT GGCACTGGGA GAGGAAGCCG TGGCCAACCT CCAGTTCTGG 
CTGCTCAACA TTGAGGCCAA GGCCCCAAAT GCCGTTGTGC TGGTGGTCGG GACACACCTG 
GACCTAATCG AAGCTAAGTT CCGAGTGGAG AGGATAGCAA CCCTGCGTGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCATC GGGCTCCAGG GCTACGGGCT TCCCGGACAT CACTTTCAAA 
CACTTGCATG AGATCTCCTG CAAGAATCTG GAAGGGCAGG AGGGGCTGCG GCAACTGATC 
TTCCACGTCA CGTGCAACAT GAAGGATGTG GGTAGCACTA TCGGCTGTCA AAAACTCGCC 
GGCAGGCTGA TCCCCAGGAG CTACATAAGC CTACAGGAGG CCGTGCTGGC TGAGCAACAG 
CGCCGCAGTC TGGGTGACCA AGTACAGTAT CTGACGGACA GGCAGCTGGA CCAGCTGGTA 
GAGCAGACGC CTGGCAATGA TATCAAAGAC TATGAAGACC TGCAGTCTGC CATCAGCTTC 
CTCATAGAAA CTGGGACCCT GCTGCACTTT CCAGACACGA GCCATGGCCT AAGGAACCTC 
TACTTCCTGG ATCCCATCTG GCTCTCTGAA TGCCTGCAGA GGATCTTTAA CATCAAGGGC 
TCGCGGTCAG TGGCAAAGAA TGGGGTGATC CAAGCGGAAG ACCTCAGGAT GCTGCTGGTG 
GGGACAGGCT TCACACAGCA GACTGAGGAG CAATACTTCC AGTTCCTTGC CAAGTTCGAG 
ATTGCCCTCC CTGTTGCCAA TGACAGCTAC CTCCTACCAC ACCTCCTTCC ATCCAAGCCT 
GGGCTGGACA CCCACAGCAT GCGGCACCCA ATGGCTAACA CCATCCAGCG GGTGTTTAAG 
ATGAGCTTCG TGCCTGTTGG CTTCTGGCAA CGGTTCATAG CACGGATGCT AATCAGCTTG 
GCTGAGATGG ACCTGCAGCT TTTTGAAAAC AAGAAGAATA CGAAAAGCAG GAACCGGAAA 
GTCACTATTT ACAGTTTTAC AGGGAGCCAG AGAAACCGCT GCAGTACATT CCGAGTCCGA 
AGAAATCAGA CCATCTACTG GCAGGAAGGG CTGCTGGTCA CTTTCGATGG GGGCTACCTC 
AGTGTGGAAT CCTCAGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT TAAAATCATC 
TGCCAGTCAG AGATGAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAACTCC 
CTGATTGACC AGTGGTTCCC TGCCCTGACG GCCACAGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTACG TACCCTGCCC TGTCTGTGAA GCATCCTGGG CCCAGCATGC AGATCCAAAC 
GAGAGGTCAG AGAGCGTGCA GTACTTTGAT ATGGAAGACT GTGTGCTCAC AGCCATCGAA 
CGGGACTTCA TCTCCTGCCC CAGACACCCA GACCTCCCAG TACCACTTCA GGAGCTTGTA 
CCAGAGCTGT TCATGACTGA CTTCCCAGCC AGGCTCTTCC TGGAGAACAG CAAGCTGGAG 
CACACGGAGG GTGAGAACAG CATTCTGGGC CAAGGTGGCA GTGGCACAGT CATCTACCAG 
GCCCGGTACC AGGGCCAGCC TGTGGCTGTG AAGAGGTTCC ATATCAAGAA GTTCAAGAAT 
TCTGCTAATG CCCCAGCAGA CACCATGCTG CGGCACCTGA GAGCCATGGA TGCCATGAAG 
AACTTCTCAG ATTTCCGTCA GGAGGCCAGC ATGCTGCATG CCTTGCAGCA TCCTTGCATC 
GTGTCACTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCTTGGAGCT GGCCCCGCTG 
GGCAGTCTCA ACACCGTGCT ATCTGAGAAT GCCAAAGATT CTTCCTTTAT GCCCCTGGGA 
CACATGCTCA CCCAAAAGAT AGCCTACCAG ATTGCCTCGG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTCAAGTCG GACAACATCC TAGTGTGGTC GCTGAGCGCC 
AAGGAGCATA TCAACATCAA ACTGTCAGAC TACGGCATCT CCAGGCAGTC CTTTCACGAG 
GGGGCCCTGG GTGTGGAGGG CACCCCAGGT TACCAGGCCC CAGAGATCAG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA TATGTTCTCC TACGGCATGG TGCTATACGA GCTGCTGTCA 
GGACAGCGTC CTGCACTGGG CCACCACCAA CTTCAGATTG TCAAGAAGTT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGGCA GCCGGAGGAA GTTCAGTTCC ATCGGCTGCA GGCGCTCATG 
ATGGAGTGCT GGGACACAAA GCCGGAGAAG CGCCCCCTGG CCTTGTCCGT GGTGAGCCAG 
ATGAAAGACC CAACCTTTGC CACCTTCATG TACATGCTGC CCTGTGGGAA GCAGTCGGCC 
TTCTTCTCAT CCCAGAGCCA GGAGTACACT GTGGTGTTCT GGGATGGGAA GGAGGAGTCC 
AGGAATTACA CGGTGGTCAA CACAGAGAAG GGCCTTCTGG AAGTACAGAG GATGACCTGC 
CCCGGGATGA AGTTGAGCTG TCAGCTCAAG GTCCAGAGCT CAGTGTGGAT AGCCACGGAG 
GACCAGAAAA TCTATATCTA CAGCCTTAAG GGTATGTGCC CATTAAGCGT GCCCCAGCAG 
GCCCTGGACA CCCCAGCTGT CGTCACCTGT TTCTTGGCAG TACCTGTTAT CAAAAAGAAC 
TCCTTCCTGG TGCTGGCAGG CCTGGCTGAC GGACTCGTGG CTGTGTTTCC TGTGGCACGG 
GGCACCCCAA AAGAAAGCTG CTCCTACCTG TGCTCCCACA CAGCCAACAG GTCCAAGTTC 
TGCATCCCCG ACGAAGATGC ACGGCAGAAC CCTTACCCAG TGAAGGCAAT GGAAGTGGTC 
AACAGTGGGT CTGAGGTCTG GTACAGCAAC GGGCCTGGCC TCCTCGTCAT TGACTGCACC 
ATCCTGGACA TCAGCAGGCG GCTGGAGCCC TACGCAGCTC CGTCCATGGT CACGTCACTT 
GTGTGCAGCT CAGACTGCAG AGGAGAGGAG ATGGTCTGGT GCCTCGACGA CAAGGCCAAT 
TGCTTAGTGA TGTACCACTC AGCCACCTAC CAGCTCTGTG CCCGGTACTT CTGTGGGGAC 
CCCAATCCTC TCAGGGACAC GTTTTCTGTG CAGCCTTCAG TCCTAGAAAC CCCAGGCAGT 
CACAAAACCA CCTCAAAGGG GCCTGTGGAA GAGTGCATCG CGGATGTGAG CATCATGTAC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTCACCGA CTACTGCTCC 
ATGTCCTCTT ATTCTTCGTC CCCACCCCAC CAAGATCCTA GGTCCCCTTC CAGTCTGCCC 
AGCTCCCTCA CCAGCTATTC CAGTGTGCCT TTCTCTGCCA ACTATGAAGA CTCAGACAGG 
CTGCAGGAGC CCAGTGTCAC CTCTGACAGA ACTGAGCATG ACCTGAGCCC CATGGATGGG 
GAGACGTTCA GCCAGCACCT GCAGGCTGTG AAGGTGCTTG CTGTCAAAGA CCTCATTTGG 
GTTCCTAGAC ATGGAGGAGA TATCATTGTC ATCGGCCTGG AGAAGGATTC AGGTGCCCAG 
CGGGGCAGAG TCATCGCTGT TTTAAAAGCC CGAGAGCTCA ATCGGCACGG GGTTCTGGTG 
GATGCCGCAG TGGTGGCAAA GGACACCGTA GTGTGCAGCT TTGCAAATGA AAACACAGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC GCCAGGGAGT TTGACATCTT CTACCAGTCC 
TATGAGGAGC TGGGCCGGCT GGAGGCTTGT ACTCGCAAGA GAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006540901.1
CDS	169..6216
Translation	MPSRMAGGPQRYRSLLTGAFQDSLPNPQPCDVMGAEDMGSKLSLPGGSSTVQCPSMEEIHTAYKQRNLSRARDLLRGVCEESESSQEKGQLLSIAAAHGDLETVQFLLTEKRVELPTEPTDDNPAVVAAHFGHAEVVRELLESLPGPCTPQRLLNWMLALACQRGHLEVVKLLVLTHGADPENYAVRKNEFPVIVRLPLYAAIKAGNEDIAIFLLRHGAYFCSYILLDSPEPSKHLLRKYFIEASALPSSYPGKIALRVKWSHLKLPWVDLDWLLDISCQITELDLSANCLPSLPSIIPWGLINLKKLNLSNNQLGELPCVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNYLERLFEEENATNWIGLRKLQELDLADNRLTELPVQFMHSFKSLTSLNVSRNNLKSFPDPWSCPLKCCKASKNALESLPDKMAVFWKSHLRDADFSENSLKEVPLGLFQLDALMFLRLQGNQLLSLPPQEKWTCTQLKTLDLSRNQLGKNEDGLKTKRISLFTTRGRQRSGTETASMLEFPAFLSESLEVLCLNDNHLDAVPPSVCLLKNLSELYLGNNPGLRELPPELGQLGNLWQLDIEDLNISNVPAEVRKEGPKATLSFLRAQLRKAEKCKLMKMILVGPPRQGKSTLLEILQTGKAPQLAHSEATIRTTKWELQRPAGSKAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKNLEGQEGLRQLIFHVTCNMKDVGSTIGCQKLAGRLIPRSYISLQEAVLAEQQRRSLGDQVQYLTDRQLDQLVEQTPGNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIQAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHSMRHPMANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGSQRNRCSTFRVRRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGIKIICQSEMRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCEASWAQHADPNERSESVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHTEGENSILGQGGSGTVIYQARYQGQPVAVKRFHIKKFKNSANAPADTMLRHLRAMDAMKNFSDFRQEASMLHALQHPCIVSLIGISIHPLCFALELAPLGSLNTVLSENAKDSSFMPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLSAKEHINIKLSDYGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIVKKLSKGIRPVLGQPEEVQFHRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYMLPCGKQSAFFSSQSQEYTVVFWDGKEESRNYTVVNTEKGLLEVQRMTCPGMKLSCQLKVQSSVWIATEDQKIYIYSLKGMCPLSVPQQALDTPAVVTCFLAVPVIKKNSFLVLAGLADGLVAVFPVARGTPKESCSYLCSHTANRSKFCIPDEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCTILDISRRLEPYAAPSMVTSLVCSSDCRGEEMVWCLDDKANCLVMYHSATYQLCARYFCGDPNPLRDTFSVQPSVLETPGSHKTTSKGPVEECIADVSIMYSEELGTQILTHQDSLTDYCSMSSYSSSPPHQDPRSPSSLPSSLTSYSSVPFSANYEDSDRLQEPSVTSDRTEHDLSPMDGETFSQHLQAVKVLAVKDLIWVPRHGGDIIVIGLEKDSGAQRGRVIAVLKARELNRHGVLVDAAVVAKDTVVCSFANENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR

Target ORF information:

RefSeq Version	XM_006540838.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine-rich repeat kinase 1 (Lrrk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006540838.3

ORF Insert Sequence:

ATGCCTTCCA GAATGGCTGG GGGTCCCCAG AGATATAGGA GCCTGCTGAC AGGAGCTTTT 
CAGGATTCTC TTCCTAACCC CCAGCCCTGT GATGTCATGG GTGCCGAAGA CATGGGCAGC 
AAACTGTCTT TACCAGGTGG CAGCTCCACG GTGCAGTGCC CCAGCATGGA AGAAATCCAC 
ACTGCGTACA AGCAGAGGAA CCTCTCCCGA GCCCGAGACC TACTCAGAGG GGTTTGTGAA 
GAGAGCGAAT CCTCACAGGA GAAGGGCCAG CTGCTGAGCA TCGCGGCAGC CCACGGGGAT 
CTGGAGACTG TTCAGTTCCT GCTCACTGAG AAGCGCGTGG AGCTGCCGAC GGAGCCCACC 
GATGACAACC CAGCTGTGGT GGCAGCGCAT TTTGGACACG CCGAAGTAGT ACGTGAACTA 
CTAGAGTCCT TGCCAGGTCC CTGTACCCCC CAGCGGCTAC TGAACTGGAT GCTGGCCCTG 
GCTTGCCAGC GAGGGCACCT GGAGGTTGTG AAGCTGCTGG TCCTGACACA TGGGGCTGAC 
CCGGAGAACT ATGCCGTCAG GAAGAATGAG TTCCCCGTCA TTGTGCGGTT GCCCCTCTAT 
GCGGCCATCA AGGCAGGGAA TGAAGACATT GCCATATTCC TGCTTCGGCA CGGAGCCTAT 
TTCTGCTCCT ACATCCTGCT GGATAGTCCT GAGCCAAGCA AACATCTGCT CAGGAAGTAT 
TTCATCGAAG CCAGCGCCCT GCCCAGCAGC TATCCCGGGA AAATCGCACT TCGTGTGAAA 
TGGTCCCATC TGAAGTTGCC CTGGGTGGAC CTGGACTGGC TCCTAGACAT CTCCTGCCAG 
ATCACAGAGC TTGACCTGTC TGCCAACTGC CTGCCTTCGC TCCCTTCCAT CATCCCTTGG 
GGACTCATCA ATCTAAAGAA GCTGAATCTC TCAAACAACC AGCTGGGGGA GCTGCCCTGC 
GTGCAGTCCT CCGACGAAAT CATCTGCTCC AGGTTACTTG AAATTGACAT CTCCAGCAAC 
AAACTGTCTC ACCTCCCTCC TGGATTCCTG CACCTCTCAA AACTTCAAAA GCTGACTGCT 
TCCAAAAACT ACCTGGAGCG GCTGTTTGAA GAAGAAAACG CCACTAACTG GATCGGCCTG 
CGGAAGCTGC AAGAACTCGA TCTAGCTGAC AACAGGCTGA CAGAGCTCCC TGTCCAATTT 
ATGCACTCCT TCAAGTCTCT CACCTCTCTG AATGTCTCCA GGAACAACCT GAAGAGCTTC 
CCGGATCCCT GGTCCTGCCC TTTGAAATGT TGTAAAGCCT CCAAAAATGC CCTGGAATCG 
CTGCCAGACA AAATGGCTGT CTTTTGGAAA AGCCACCTTC GGGATGCAGA TTTCTCTGAG 
AACTCTCTGA AAGAGGTGCC CCTGGGATTG TTCCAGCTTG ACGCCCTCAT GTTCTTGAGG 
TTACAGGGAA ACCAGCTGTT GTCGCTGCCA CCTCAAGAGA AGTGGACCTG CACCCAACTC 
AAGACCCTGG ACCTCTCCAG AAACCAGCTC GGCAAAAATG AAGATGGGCT TAAAACAAAG 
AGGATCTCCC TGTTCACCAC CAGAGGGCGC CAGCGTTCTG GAACTGAGAC AGCCTCCATG 
TTGGAATTCC CAGCTTTCCT AAGTGAGTCT TTGGAGGTCC TTTGTCTGAA TGACAACCAT 
CTTGATGCTG TTCCCCCATC GGTCTGCCTG CTGAAGAACC TCTCAGAGCT CTACTTGGGC 
AATAACCCTG GCCTCCGAGA GCTCCCTCCG GAGCTAGGCC AGCTGGGGAA CCTCTGGCAA 
CTGGACATTG AAGATCTGAA CATTAGCAAC GTGCCTGCGG AGGTCAGGAA AGAAGGCCCG 
AAAGCAACAC TGTCTTTCCT GCGTGCTCAG CTGCGCAAAG CAGAGAAGTG TAAGCTGATG 
AAGATGATCC TCGTGGGTCC CCCGCGCCAG GGCAAGTCCA CACTCCTGGA GATCTTACAG 
ACCGGGAAGG CCCCGCAGCT AGCACACAGT GAAGCCACCA TCAGGACCAC CAAGTGGGAG 
CTCCAGAGAC CAGCAGGCTC CAAAGCCAAG GTTGAGTCCG TAGAGTTCAA CGTCTGGGAC 
ATCGGGGGTC CGGCCAGCAT GGCCACGGTC AATCAGTGCT TCTTCACAGA CAAGGCGCTG 
TATGTGGTGG TCTGGAACCT GGCACTGGGA GAGGAAGCCG TGGCCAACCT CCAGTTCTGG 
CTGCTCAACA TTGAGGCCAA GGCCCCAAAT GCCGTTGTGC TGGTGGTCGG GACACACCTG 
GACCTAATCG AAGCTAAGTT CCGAGTGGAG AGGATAGCAA CCCTGCGTGC CTACGTGCTG 
GCCCTCTGCC GCTCGCCATC GGGCTCCAGG GCTACGGGCT TCCCGGACAT CACTTTCAAA 
CACTTGCATG AGATCTCCTG CAAGAATCTG GAAGGGCAGG AGGGGCTGCG GCAACTGATC 
TTCCACGTCA CGTGCAACAT GAAGGATGTG GGTAGCACTA TCGGCTGTCA AAAACTCGCC 
GGCAGGCTGA TCCCCAGGAG CTACATAAGC CTACAGGAGG CCGTGCTGGC TGAGCAACAG 
CGCCGCAGTC TGGGTGACCA AGTACAGTAT CTGACGGACA GGCAGCTGGA CCAGCTGGTA 
GAGCAGACGC CTGGCAATGA TATCAAAGAC TATGAAGACC TGCAGTCTGC CATCAGCTTC 
CTCATAGAAA CTGGGACCCT GCTGCACTTT CCAGACACGA GCCATGGCCT AAGGAACCTC 
TACTTCCTGG ATCCCATCTG GCTCTCTGAA TGCCTGCAGA GGATCTTTAA CATCAAGGGC 
TCGCGGTCAG TGGCAAAGAA TGGGGTGATC CAAGCGGAAG ACCTCAGGAT GCTGCTGGTG 
GGGACAGGCT TCACACAGCA GACTGAGGAG CAATACTTCC AGTTCCTTGC CAAGTTCGAG 
ATTGCCCTCC CTGTTGCCAA TGACAGCTAC CTCCTACCAC ACCTCCTTCC ATCCAAGCCT 
GGGCTGGACA CCCACAGCAT GCGGCACCCA ATGGCTAACA CCATCCAGCG GGTGTTTAAG 
ATGAGCTTCG TGCCTGTTGG CTTCTGGCAA CGGTTCATAG CACGGATGCT AATCAGCTTG 
GCTGAGATGG ACCTGCAGCT TTTTGAAAAC AAGAAGAATA CGAAAAGCAG GAACCGGAAA 
GTCACTATTT ACAGTTTTAC AGGGAGCCAG AGAAACCGCT GCAGTACATT CCGAGTCCGA 
AGAAATCAGA CCATCTACTG GCAGGAAGGG CTGCTGGTCA CTTTCGATGG GGGCTACCTC 
AGTGTGGAAT CCTCAGATGT GAACTGGAAA AAGAAAAAAA GCGGAGGAAT TAAAATCATC 
TGCCAGTCAG AGATGAGGGA CTTCTCGGCA ATGGCTTTTA TCACAGACCA TGTCAACTCC 
CTGATTGACC AGTGGTTCCC TGCCCTGACG GCCACAGAAA GTGACGGGAC CCCACTGATG 
GAGCAGTACG TACCCTGCCC TGTCTGTGAA GCATCCTGGG CCCAGCATGC AGATCCAAAC 
GAGAGGTCAG AGAGCGTGCA GTACTTTGAT ATGGAAGACT GTGTGCTCAC AGCCATCGAA 
CGGGACTTCA TCTCCTGCCC CAGACACCCA GACCTCCCAG TACCACTTCA GGAGCTTGTA 
CCAGAGCTGT TCATGACTGA CTTCCCAGCC AGGCTCTTCC TGGAGAACAG CAAGCTGGAG 
CACACGGAGG GTGAGAACAG CATTCTGGGC CAAGGTGGCA GTGGCACAGT CATCTACCAG 
GCCCGGTACC AGGGCCAGCC TGTGGCTGTG AAGAGGTTCC ATATCAAGAA GTTCAAGAAT 
TCTGCTAATG CCCCAGCAGA CACCATGCTG CGGCACCTGA GAGCCATGGA TGCCATGAAG 
AACTTCTCAG ATTTCCGTCA GGAGGCCAGC ATGCTGCATG CCTTGCAGCA TCCTTGCATC 
GTGTCACTCA TCGGCATCAG CATCCACCCG CTCTGCTTCG CCTTGGAGCT GGCCCCGCTG 
GGCAGTCTCA ACACCGTGCT ATCTGAGAAT GCCAAAGATT CTTCCTTTAT GCCCCTGGGA 
CACATGCTCA CCCAAAAGAT AGCCTACCAG ATTGCCTCGG GCCTGGCCTA CCTGCACAAG 
AAAAACATCA TCTTCTGTGA CCTCAAGTCG GACAACATCC TAGTGTGGTC GCTGAGCGCC 
AAGGAGCATA TCAACATCAA ACTGTCAGAC TACGGCATCT CCAGGCAGTC CTTTCACGAG 
GGGGCCCTGG GTGTGGAGGG CACCCCAGGT TACCAGGCCC CAGAGATCAG GCCTCGCATC 
GTGTATGACG AGAAGGTAGA TATGTTCTCC TACGGCATGG TGCTATACGA GCTGCTGTCA 
GGACAGCGTC CTGCACTGGG CCACCACCAA CTTCAGATTG TCAAGAAGTT GTCCAAGGGC 
ATCCGCCCAG TTCTGGGGCA GCCGGAGGAA GTTCAGTTCC ATCGGCTGCA GGCGCTCATG 
ATGGAGTGCT GGGACACAAA GCCGGAGAAG CGCCCCCTGG CCTTGTCCGT GGTGAGCCAG 
ATGAAAGACC CAACCTTTGC CACCTTCATG TACATGCTGC CCTGTGGGAA GCAGTCGGCC 
TTCTTCTCAT CCCAGAGCCA GGAGTACACT GTGGTGTTCT GGGATGGGAA GGAGGAGTCC 
AGGAATTACA CGGTGGTCAA CACAGAGAAG GGCCTTCTGG AAGTACAGAG GATGACCTGC 
CCCGGGATGA AGTTGAGCTG TCAGCTCAAG GTCCAGAGCT CAGTGTGGAT AGCCACGGAG 
GACCAGAAAA TCTATATCTA CAGCCTTAAG GGTATGTGCC CATTAAGCGT GCCCCAGCAG 
GCCCTGGACA CCCCAGCTGT CGTCACCTGT TTCTTGGCAG TACCTGTTAT CAAAAAGAAC 
TCCTTCCTGG TGCTGGCAGG CCTGGCTGAC GGACTCGTGG CTGTGTTTCC TGTGGCACGG 
GGCACCCCAA AAGAAAGCTG CTCCTACCTG TGCTCCCACA CAGCCAACAG GTCCAAGTTC 
TGCATCCCCG ACGAAGATGC ACGGCAGAAC CCTTACCCAG TGAAGGCAAT GGAAGTGGTC 
AACAGTGGGT CTGAGGTCTG GTACAGCAAC GGGCCTGGCC TCCTCGTCAT TGACTGCACC 
ATCCTGGACA TCAGCAGGCG GCTGGAGCCC TACGCAGCTC CGTCCATGGT CACGTCACTT 
GTGTGCAGCT CAGACTGCAG AGGAGAGGAG ATGGTCTGGT GCCTCGACGA CAAGGCCAAT 
TGCTTAGTGA TGTACCACTC AGCCACCTAC CAGCTCTGTG CCCGGTACTT CTGTGGGGAC 
CCCAATCCTC TCAGGGACAC GTTTTCTGTG CAGCCTTCAG TCCTAGAAAC CCCAGGCAGT 
CACAAAACCA CCTCAAAGGG GCCTGTGGAA GAGTGCATCG CGGATGTGAG CATCATGTAC 
AGCGAGGAGC TGGGCACGCA GATCCTCACC CACCAGGACT CGCTCACCGA CTACTGCTCC 
ATGTCCTCTT ATTCTTCGTC CCCACCCCAC CAAGATCCTA GGTCCCCTTC CAGTCTGCCC 
AGCTCCCTCA CCAGCTATTC CAGTGTGCCT TTCTCTGCCA ACTATGAAGA CTCAGACAGG 
CTGCAGGAGC CCAGTGTCAC CTCTGACAGA ACTGAGCATG ACCTGAGCCC CATGGATGGG 
GAGACGTTCA GCCAGCACCT GCAGGCTGTG AAGGTGCTTG CTGTCAAAGA CCTCATTTGG 
GTTCCTAGAC ATGGAGGAGA TATCATTGTC ATCGGCCTGG AGAAGGATTC AGGTGCCCAG 
CGGGGCAGAG TCATCGCTGT TTTAAAAGCC CGAGAGCTCA ATCGGCACGG GGTTCTGGTG 
GATGCCGCAG TGGTGGCAAA GGACACCGTA GTGTGCAGCT TTGCAAATGA AAACACAGAG 
TGGTGCCTGG CTGTCTGGAG GGGCTGGGGC GCCAGGGAGT TTGACATCTT CTACCAGTCC 
TATGAGGAGC TGGGCCGGCT GGAGGCTTGT ACTCGCAAGA GAAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Age-Dependent Dopaminergic Neurodegeneration and Impairment of the Autophagy-Lysosomal Pathway in LRRK-Deficient Mice.
Neuron96(4)796-807.e6(2017 Nov)
Giaime E,Tong Y,Wagner LK,Yuan Y,Huang G,Shen J

Leucine-rich repeat kinase-1 regulates osteoclast function by modulating RAC1/Cdc42 Small GTPase phosphorylation and activation.
American journal of physiology. Endocrinology and metabolism311(4)E772-E780(2016 Oct)
Zeng C,Goodluck H,Qin X,Liu B,Mohan S,Xing W

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

LRRK1 is critical in the regulation of B-cell responses and CARMA1-dependent NF-?B activation.
Scientific reports625738(2016 May)
Morimoto K,Baba Y,Shinohara H,Kang S,Nojima S,Kimura T,Ito D,Yoshida Y,Maeda Y,Sarashina-Kida H,Nishide M,Hosokawa T,Kato Y,Hayama Y,Kinehara Y,Okuno T,Takamatsu H,Hirano T,Shima Y,Narazaki M,Kurosaki T,Toyofuku T,Kumanogoh A

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Lrrk1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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