Ap2s1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap2s1
Entrez Gene ID	232910
Full Name	adaptor-related protein complex 2, sigma 1 subunit
Synonyms	AI043088
General protein information	Preferred Names adaptor-related protein complex 2, sigma 1 subunit Names AP-2 complex subunit sigma adapter-related protein complex 2 sigma subunit adapter-related protein complex 2 subunit sigma adaptor protein complex AP-2 subunit sigma adaptor-related protein complex 2 subunit sigma clathrin assembly protein 2 sigma small chain clathrin assembly protein 2 small chain clathrin coat assembly protein AP17 clathrin coat-associated protein AP17 plasma membrane adaptor AP-2 17 kDa protein sigma-adaptin 3b sigma2-adaptin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_198613.2	NP_941015.2	AP-2 complex subunit sigma

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AP2S1	NP_001069546.1
Gallus gallus (chicken)	LOC100859555	XP_003643618.2
Danio rerio (zebrafish)	ap2s1	NP_998320.1
Schizosaccharomyces pombe (fission yeast)	aps2	NP_596138.1
Pan troglodytes (chimpanzee)	AP2S1	XP_003953587.1
Magnaporthe oryzae (rice blast fungus)	MGG_01762	XP_003714763.1
Drosophila melanogaster (fruit fly)	AP-2sigma	NP_650961.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001703	XP_321389.2
Caenorhabditis elegans (roundworm)	aps-2	NP_508767.1
Kluyveromyces lactis	KLLA0B06545g	XP_451826.1
Eremothecium gossypii	AGOS_AFR370C	NP_985917.1
Arabidopsis thaliana (thale cress)	AT1G47830	NP_175219.1
Xenopus tropicalis (tropical clawed frog)	ap2s1	NP_001016325.1
Homo sapiens (human)	AP2S1	NP_004060.2
	AP2S1	XP_001112688.1
Canis lupus familiaris (dog)	AP2S1	XP_533634.1
Mus musculus (house mouse)	Ap2s1	NP_941015.2
Rattus norvegicus (Norway rat)	Ap2s1	NP_075241.2
Saccharomyces cerevisiae (baker's yeast)	APS2	NP_012592.1
Neurospora crassa	NCU07989	XP_962659.1
Oryza sativa (rice)	Os12g0207300	NP_001066387.1

Gene Ontology
Bibliography

Related articles in PubMed

Otoferlin couples to clathrin-mediated endocytosis in mature cochlear inner hair cells.
Duncker SV, Franz C, Kuhn S, Schulte U, Campanelli D, Brandt N, Hirt B, Fakler B, Blin N, Ruth P, Engel J, Marcotti W, Zimmermann U, Knipper M
The Journal of neuroscience : the official journal of the Society for Neuroscience33(22)9508-19(2013 May)

Molecular architecture and functional model of the endocytic AP2 complex.
Collins BM, McCoy AJ, Kent HM, Evans PR, Owen DJ
Cell109(4)523-35(2002 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ap2s1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap2s1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09068	NM_198613.2 Latest version!	Mus musculus adaptor-related protein complex 2, sigma 1 subunit (Ap2s1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap2s1 ( NM_198613.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_941015.2 Mus musculus adaptor-related protein complex 2, sigma 1 subunit (Ap2s1), mRNA.

Price & Availability↑

CloneID	OMu09068
Clone ID Related Accession (Same CDS sequence)	NM_198613.2
Accession Version	NM_198613.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	AP-2 complex subunit sigma
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK146584.1. On Nov 27, 2007 this sequence version replaced NM_198613.1. ##Evidence-Data-START## Transcript exon combination :: AK146584.1, BU604137.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCCGAT TCATCCTTAT CCAGAACCGG GCAGGCAAGA CGCGCCTGGC CAAGTGGTAC 
ATGCAGTTCG ATGACGACGA GAAGCAGAAG CTGATCGAGG AGGTGCACGC CGTGGTCACC 
GTCAGGGATG CCAAGCACAC CAACTTTGTG GAGTTCCGGA ACTTCAAGAT CATCTACCGA 
CGCTACGCTG GCCTCTACTT CTGCATCTGC GTGGATGTCA ACGACAACAA TCTGGCCTAT 
CTCGAGGCCA TCCACAACTT CGTAGAAGTG TTAAATGAAT ACTTCCACAA TGTCTGTGAA 
CTGGACCTGG TGTTCAACTT CTACAAGGTT TACACGGTGG TAGATGAGAT GTTCCTGGCA 
GGAGAGATCC GAGAGACCAG CCAGACGAAG GTGCTGAAGC AGCTGCTGAT GCTGCAGTCC 
CTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_941015.2
CDS	93..521
Translation	MIRFILIQNRAGKTRLAKWYMQFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIIYRRYAGLYFCICVDVNDNNLAYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLLMLQSLE

Target ORF information:

RefSeq Version	NM_198613.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adaptor-related protein complex 2, sigma 1 subunit (Ap2s1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198613.2

ORF Insert Sequence:

ATGATCCGAT TCATCCTTAT CCAGAACCGG GCAGGCAAGA CGCGCCTGGC CAAGTGGTAC 
ATGCAGTTCG ATGACGACGA GAAGCAGAAG CTGATCGAGG AGGTGCACGC CGTGGTCACC 
GTCAGGGATG CCAAGCACAC CAACTTTGTG GAGTTCCGGA ACTTCAAGAT CATCTACCGA 
CGCTACGCTG GCCTCTACTT CTGCATCTGC GTGGATGTCA ACGACAACAA TCTGGCCTAT 
CTCGAGGCCA TCCACAACTT CGTAGAAGTG TTAAATGAAT ACTTCCACAA TGTCTGTGAA 
CTGGACCTGG TGTTCAACTT CTACAAGGTT TACACGGTGG TAGATGAGAT GTTCCTGGCA 
GGAGAGATCC GAGAGACCAG CCAGACGAAG GTGCTGAAGC AGCTGCTGAT GCTGCAGTCC 
CTGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Otoferlin couples to clathrin-mediated endocytosis in mature cochlear inner hair cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(22)9508-19(2013 May)
Duncker SV,Franz C,Kuhn S,Schulte U,Campanelli D,Brandt N,Hirt B,Fakler B,Blin N,Ruth P,Engel J,Marcotti W,Zimmermann U,Knipper M

Molecular architecture and functional model of the endocytic AP2 complex.
Cell109(4)523-35(2002 May)
Collins BM,McCoy AJ,Kent HM,Evans PR,Owen DJ