Clic5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Clic5
Entrez Gene ID	224796
Full Name	chloride intracellular channel 5
Synonyms	5730531E12Rik,B330005L24,Gm322,jbg,nmf318
General protein information	Preferred Names chloride intracellular channel 5 Names chloride intracellular channel protein 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524135.4	XP_006524198.1	chloride intracellular channel protein 5 isoform X1
NM_172621.2	NP_766209.1	chloride intracellular channel protein 5
XM_006524135.3	XP_006524198.1	chloride intracellular channel protein 5 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLIC5	XP_001142911.1
Xenopus tropicalis (tropical clawed frog)	clic4	NP_001006831.1
Mus musculus (house mouse)	Clic5	NP_766209.1
Drosophila melanogaster (fruit fly)	Clic	NP_572928.1
Homo sapiens (human)	CLIC5	NP_001107558.1
	CLIC5	XP_001101512.1
Canis lupus familiaris (dog)	CLIC5	XP_538943.2
Bos taurus (cattle)	CLIC5	NP_776701.1
Rattus norvegicus (Norway rat)	Clic5	NP_446055.1
Gallus gallus (chicken)	CLIC5	XP_420060.2
Danio rerio (zebrafish)	clic5a	XP_005169863.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000943	XP_003437145.1

Gene Ontology
Bibliography

Related articles in PubMed

CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.
Tang T, Lang X, Xu C, Wang X, Gong T, Yang Y, Cui J, Bai L, Wang J, Jiang W, Zhou R
Nature communications8(1)202(2017 08)

Both CLIC4 and CLIC5A activate ERM proteins in glomerular endothelium.
Tavasoli M, Al-Momany A, Wang X, Li L, Edwards JC, Ballermann BJ
American journal of physiology. Renal physiology311(5)F945-F957(2016 Nov)

Emerging biological roles of Cl- intracellular channel proteins.
Argenzio E, Moolenaar WH
Journal of cell science129(22)4165-4174(2016 11)

The chloride intracellular channel 5A stimulates podocyte Rac1, protecting against hypertension-induced glomerular injury.
Tavasoli M, Li L, Al-Momany A, Zhu LF, Adam BA, Wang Z, Ballermann BJ
Kidney international89(4)833-47(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate CLICs-dependent chloride efflux as an essential and proximal upstream event for NLRP3 activation.
Title: CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.

The findings indicate that CLIC4/CLIC5A-mediated ERM activation is required for maintenance of the glomerular capillary architecture.
Title: Both CLIC4 and CLIC5A activate ERM proteins in glomerular endothelium.

Augmented hypertension-induced glomerular capillary injury in mice lacking CLIC5 results from abrogation of Rac1-dependent Pak and ezrin activation, perhaps reducing the tensile strength of the podocyte actin cytoskeleton.
Title: The chloride intracellular channel 5A stimulates podocyte Rac1, protecting against hypertension-induced glomerular injury.

The mechanism of CLIC5A action involves clustered plasma membrane phosphatidylinositol 4,5-bisphosphate accumulation, in turn facilitating ezrin activation and actin-dependent cell surface remodeling.
Title: Clustered PI(4,5)P? accumulation and ezrin phosphorylation in response to CLIC5A.

Biochemical assays showed interaction of CLIC5 with ERM proteins, TPRN, and possibly myosin VI (MYO6). In addition, CLIC5 and RDX failed to localize normally in fused stereocilia of MYO6 mutant mice
Title: CLIC5 stabilizes membrane-actin filament linkages at the base of hair cell stereocilia in a molecular complex with radixin, taperin, and myosin VI.

The following Clic5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Clic5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18046	NM_172621.2 Latest version!	Mus musculus chloride intracellular channel 5 (Clic5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu53491	XM_006524135.4 Latest version!	Mus musculus chloride intracellular channel 5 (Clic5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu53491	XM_006524135.3	Mus musculus chloride intracellular channel 5 (Clic5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Clic5 ( NM_172621.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766209.1 Mus musculus chloride intracellular channel 5 (Clic5), mRNA.

Price & Availability↑

CloneID	OMu18046
Clone ID Related Accession (Same CDS sequence)	NM_172621.2
Accession Version	NM_172621.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	chloride intracellular channel protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116688.14, AK156849.1 and AC153418.1. On Jan 21, 2006 this sequence version replaced NM_172621.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK156849.1, AK046522.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGACGGACT CAGCGACAAC TAATGGGGAC GACAGGGACC CCGAGATCGA GCTCTTCGTG 
AAGGCTGGGA TCGACGGGGA AAGCATTGGC AACTGTCCCT TCTCTCAGCG TCTCTTCATG 
ATCCTCTGGC TGAAAGGAGT CGTGTTCAAT GTCACCACCG TGGATCTGAA AAGAAAGCCA 
GCCGATCTAC ACAACCTGGC TCCTGGCACG CACCCGCCCT TCCTGACCTT CAATGGGGAT 
GTGAAGACAG ATGTCAACAA GATTGAAGAG TTCCTGGAGG AGACCCTAAC CCCCGAGAAG 
TACCCCAAAC TGGCTGCAAA ACACCGGGAA TCTAACACCG CGGGCATCGA CATCTTCTCC 
AAGTTCTCAG CCTACATCAA AAACACCAAA CAACAGAACA ATGCTGCCCT TGAGAGAGGC 
TTGACAAAGG CGCTGAGGAA GCTGGATGAC TACCTAAACA GCCCTCTGCC AGAGGAGATT 
GACACCAACA CCCACGGGGA CGAGAAGGGG TCCCAGCGCA AGTTCCTGGA TGGGGATGAG 
CTGACCCTGG CCGACTGCAA TCTGCTGCCC AAGCTGCATG TGGTCAAGAT TGTGGCTAAG 
AAGTACCGAA ACTATGACAT CCCAGCTGAG ATGACAGGCT TGTGGCGATA CCTCAAGAAT 
GCCTATGCAC GGGACGAGTT CACCAACACC TGTGCAGCTG ACAGTGAGAT CGAGTTGGCC 
TACGCAGATG TCGCCAGGCG CCTCAGCCGA TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766209.1
CDS	328..1083
Translation	MTDSATTNGDDRDPEIELFVKAGIDGESIGNCPFSQRLFMILWLKGVVFNVTTVDLKRKPADLHNLAPGTHPPFLTFNGDVKTDVNKIEEFLEETLTPEKYPKLAAKHRESNTAGIDIFSKFSAYIKNTKQQNNAALERGLTKALRKLDDYLNSPLPEEIDTNTHGDEKGSQRKFLDGDELTLADCNLLPKLHVVKIVAKKYRNYDIPAEMTGLWRYLKNAYARDEFTNTCAADSEIELAYADVARRLSRS

Target ORF information:

RefSeq Version	NM_172621.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride intracellular channel 5 (Clic5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172621.2

ORF Insert Sequence:

ATGACGGACT CAGCGACAAC TAATGGGGAC GACAGGGACC CCGAGATCGA GCTCTTCGTG 
AAGGCTGGGA TCGACGGGGA AAGCATTGGC AACTGTCCCT TCTCTCAGCG TCTCTTCATG 
ATCCTCTGGC TGAAAGGAGT CGTGTTCAAT GTCACCACCG TGGATCTGAA AAGAAAGCCA 
GCCGATCTAC ACAACCTGGC TCCTGGCACG CACCCGCCCT TCCTGACCTT CAATGGGGAT 
GTGAAGACAG ATGTCAACAA GATTGAAGAG TTCCTGGAGG AGACCCTAAC CCCCGAGAAG 
TACCCCAAAC TGGCTGCAAA ACACCGGGAA TCTAACACCG CGGGCATCGA CATCTTCTCC 
AAGTTCTCAG CCTACATCAA AAACACCAAA CAACAGAACA ATGCTGCCCT TGAGAGAGGC 
TTGACAAAGG CGCTGAGGAA GCTGGATGAC TACCTAAACA GCCCTCTGCC AGAGGAGATT 
GACACCAACA CCCACGGGGA CGAGAAGGGG TCCCAGCGCA AGTTCCTGGA TGGGGATGAG 
CTGACCCTGG CCGACTGCAA TCTGCTGCCC AAGCTGCATG TGGTCAAGAT TGTGGCTAAG 
AAGTACCGAA ACTATGACAT CCCAGCTGAG ATGACAGGCT TGTGGCGATA CCTCAAGAAT 
GCCTATGCAC GGGACGAGTT CACCAACACC TGTGCAGCTG ACAGTGAGAT CGAGTTGGCC 
TACGCAGATG TCGCCAGGCG CCTCAGCCGA TCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clic5 ( XM_006524135.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524198.1 Mus musculus chloride intracellular channel 5 (Clic5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53491
Clone ID Related Accession (Same CDS sequence)	XM_006524135.4 , XM_006524135.3
Accession Version	XM_006524135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	chloride intracellular channel protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524135.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAAG ACTACAGTGA TACCTATGAT AAAATCCAAA AGAGGAGCAC AGATGAGGCT 
CCAGACCTGC AGGAGGAAAG TGACGCTCCT TTGTATGACA GAGTCCATGA AGACTCTGAA 
AATGATCTGT ATGCCACCAG GCTAGAAAGC CAGGAGTACG AATACTTGTC ACCGTATGTC 
TCGAAAAGTG ATGAAGCCAC CCCAGCCTCC CTGGAGCCAG AAGCAGATGG CTACCTCCTG 
CCGGATGAGG TGTTTTCAGA GTCCCGGGCG GTTCGGGATG TCTCCATGGG AGACTCACAT 
TCACCAAGCC AGGACCCAGA GCCTCTCTCC TGGGAACCTC AGGAGAATGG AGGAGCAACC 
GAGGAAGACG TGCCTTCCTC ACATTCACTA AGCCAGGACC CGGAGCGTCT CCCGTGGGAA 
CCTCAGGAGA ATGGAGGAGC AACCGAGGAA GATGTGCCTT CCTCACATTC ACTAAGCCAG 
GACCTGGAGC GTCTCCCGTG GGAACCTCAG GAGAATGGAG GAGCAACCGA GGAAGACGTG 
TCTTCCTCAC ATTCACTAAG CCAGGACCTG GAGCGTCTCC CGTGGGAACC TCAGGAGAAT 
GGAGGAGCAA CCGAGGAAGA CGTGCCTTCC TTACTGAGCT TCAGGGTTCA GCACAGCAAG 
GTGCTTGCCA CAAGCCAGGA TTCCTCTATC AGCCTTTCTG CTGCTATTGG GTTGGGAGAA 
AAAGAAGCAG CTTCCAGCAA CCCTGAGATC TTCCTGTTTG TGAAGGCTGG GATCGACGGG 
GAAAGCATTG GCAACTGTCC CTTCTCTCAG CGTCTCTTCA TGATCCTCTG GCTGAAAGGA 
GTCGTGTTCA ATGTCACCAC CGTGGATCTG AAAAGAAAGC CAGCCGATCT ACACAACCTG 
GCTCCTGGCA CGCACCCGCC CTTCCTGACC TTCAATGGGG ATGTGAAGAC AGATGTCAAC 
AAGATTGAAG AGTTCCTGGA GGAGACCCTA ACCCCCGAGA AGTACCCCAA ACTGGCTGCA 
AAACACCGGG AATCTAACAC CGCGGGCATC GACATCTTCT CCAAGTTCTC AGCCTACATC 
AAAAACACCA AACAACAGAA CAATGCTGCC CTTGAGAGAG GCTTGACAAA GGCGCTGAGG 
AAGCTGGATG ACTACCTAAA CAGCCCTCTG CCAGAGGAGA TTGACACCAA CACCCACGGG 
GACGAGAAGG GGTCCCAGCG CAAGTTCCTG GATGGGGATG AGCTGACCCT GGCCGACTGC 
AATCTGCTGC CCAAGCTGCA TGTGGTCAAG ATTGTGGCTA AGAAGTACCG AAACTATGAC 
ATCCCAGCTG AGATGACAGG CTTGTGGCGA TACCTCAAGA ATGCCTATGC ACGGGACGAG 
TTCACCAACA CCTGTGCAGC TGACAGTGAG ATCGAGTTGG CCTACGCAGA TGTCGCCAGG 
CGCCTCAGCC GATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524198.1
CDS	129..1586
Translation	MNEDYSDTYDKIQKRSTDEAPDLQEESDAPLYDRVHEDSENDLYATRLESQEYEYLSPYVSKSDEATPASLEPEADGYLLPDEVFSESRAVRDVSMGDSHSPSQDPEPLSWEPQENGGATEEDVPSSHSLSQDPERLPWEPQENGGATEEDVPSSHSLSQDLERLPWEPQENGGATEEDVSSSHSLSQDLERLPWEPQENGGATEEDVPSLLSFRVQHSKVLATSQDSSISLSAAIGLGEKEAASSNPEIFLFVKAGIDGESIGNCPFSQRLFMILWLKGVVFNVTTVDLKRKPADLHNLAPGTHPPFLTFNGDVKTDVNKIEEFLEETLTPEKYPKLAAKHRESNTAGIDIFSKFSAYIKNTKQQNNAALERGLTKALRKLDDYLNSPLPEEIDTNTHGDEKGSQRKFLDGDELTLADCNLLPKLHVVKIVAKKYRNYDIPAEMTGLWRYLKNAYARDEFTNTCAADSEIELAYADVARRLSRS

Target ORF information:

RefSeq Version	XM_006524135.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride intracellular channel 5 (Clic5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524135.4

ORF Insert Sequence:

ATGAATGAAG ACTACAGTGA TACCTATGAT AAAATCCAAA AGAGGAGCAC AGATGAGGCT 
CCAGACCTGC AGGAGGAAAG TGACGCTCCT TTGTATGACA GAGTCCATGA AGACTCTGAA 
AATGATCTGT ATGCCACCAG GCTAGAAAGC CAGGAGTACG AATACTTGTC ACCGTATGTC 
TCGAAAAGTG ATGAAGCCAC CCCAGCCTCC CTGGAGCCAG AAGCAGATGG CTACCTCCTG 
CCGGATGAGG TGTTTTCAGA GTCCCGGGCG GTTCGGGATG TCTCCATGGG AGACTCACAT 
TCACCAAGCC AGGACCCAGA GCCTCTCTCC TGGGAACCTC AGGAGAATGG AGGAGCAACC 
GAGGAAGACG TGCCTTCCTC ACATTCACTA AGCCAGGACC CGGAGCGTCT CCCGTGGGAA 
CCTCAGGAGA ATGGAGGAGC AACCGAGGAA GATGTGCCTT CCTCACATTC ACTAAGCCAG 
GACCTGGAGC GTCTCCCGTG GGAACCTCAG GAGAATGGAG GAGCAACCGA GGAAGACGTG 
TCTTCCTCAC ATTCACTAAG CCAGGACCTG GAGCGTCTCC CGTGGGAACC TCAGGAGAAT 
GGAGGAGCAA CCGAGGAAGA CGTGCCTTCC TTACTGAGCT TCAGGGTTCA GCACAGCAAG 
GTGCTTGCCA CAAGCCAGGA TTCCTCTATC AGCCTTTCTG CTGCTATTGG GTTGGGAGAA 
AAAGAAGCAG CTTCCAGCAA CCCTGAGATC TTCCTGTTTG TGAAGGCTGG GATCGACGGG 
GAAAGCATTG GCAACTGTCC CTTCTCTCAG CGTCTCTTCA TGATCCTCTG GCTGAAAGGA 
GTCGTGTTCA ATGTCACCAC CGTGGATCTG AAAAGAAAGC CAGCCGATCT ACACAACCTG 
GCTCCTGGCA CGCACCCGCC CTTCCTGACC TTCAATGGGG ATGTGAAGAC AGATGTCAAC 
AAGATTGAAG AGTTCCTGGA GGAGACCCTA ACCCCCGAGA AGTACCCCAA ACTGGCTGCA 
AAACACCGGG AATCTAACAC CGCGGGCATC GACATCTTCT CCAAGTTCTC AGCCTACATC 
AAAAACACCA AACAACAGAA CAATGCTGCC CTTGAGAGAG GCTTGACAAA GGCGCTGAGG 
AAGCTGGATG ACTACCTAAA CAGCCCTCTG CCAGAGGAGA TTGACACCAA CACCCACGGG 
GACGAGAAGG GGTCCCAGCG CAAGTTCCTG GATGGGGATG AGCTGACCCT GGCCGACTGC 
AATCTGCTGC CCAAGCTGCA TGTGGTCAAG ATTGTGGCTA AGAAGTACCG AAACTATGAC 
ATCCCAGCTG AGATGACAGG CTTGTGGCGA TACCTCAAGA ATGCCTATGC ACGGGACGAG 
TTCACCAACA CCTGTGCAGC TGACAGTGAG ATCGAGTTGG CCTACGCAGA TGTCGCCAGG 
CGCCTCAGCC GATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Clic5 ( XM_006524135.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524198.1 Mus musculus chloride intracellular channel 5 (Clic5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu53491
Clone ID Related Accession (Same CDS sequence)	XM_006524135.4 , XM_006524135.3
Accession Version	XM_006524135.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	chloride intracellular channel protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAAG ACTACAGTGA TACCTATGAT AAAATCCAAA AGAGGAGCAC AGATGAGGCT 
CCAGACCTGC AGGAGGAAAG TGACGCTCCT TTGTATGACA GAGTCCATGA AGACTCTGAA 
AATGATCTGT ATGCCACCAG GCTAGAAAGC CAGGAGTACG AATACTTGTC ACCGTATGTC 
TCGAAAAGTG ATGAAGCCAC CCCAGCCTCC CTGGAGCCAG AAGCAGATGG CTACCTCCTG 
CCGGATGAGG TGTTTTCAGA GTCCCGGGCG GTTCGGGATG TCTCCATGGG AGACTCACAT 
TCACCAAGCC AGGACCCAGA GCCTCTCTCC TGGGAACCTC AGGAGAATGG AGGAGCAACC 
GAGGAAGACG TGCCTTCCTC ACATTCACTA AGCCAGGACC CGGAGCGTCT CCCGTGGGAA 
CCTCAGGAGA ATGGAGGAGC AACCGAGGAA GATGTGCCTT CCTCACATTC ACTAAGCCAG 
GACCTGGAGC GTCTCCCGTG GGAACCTCAG GAGAATGGAG GAGCAACCGA GGAAGACGTG 
TCTTCCTCAC ATTCACTAAG CCAGGACCTG GAGCGTCTCC CGTGGGAACC TCAGGAGAAT 
GGAGGAGCAA CCGAGGAAGA CGTGCCTTCC TTACTGAGCT TCAGGGTTCA GCACAGCAAG 
GTGCTTGCCA CAAGCCAGGA TTCCTCTATC AGCCTTTCTG CTGCTATTGG GTTGGGAGAA 
AAAGAAGCAG CTTCCAGCAA CCCTGAGATC TTCCTGTTTG TGAAGGCTGG GATCGACGGG 
GAAAGCATTG GCAACTGTCC CTTCTCTCAG CGTCTCTTCA TGATCCTCTG GCTGAAAGGA 
GTCGTGTTCA ATGTCACCAC CGTGGATCTG AAAAGAAAGC CAGCCGATCT ACACAACCTG 
GCTCCTGGCA CGCACCCGCC CTTCCTGACC TTCAATGGGG ATGTGAAGAC AGATGTCAAC 
AAGATTGAAG AGTTCCTGGA GGAGACCCTA ACCCCCGAGA AGTACCCCAA ACTGGCTGCA 
AAACACCGGG AATCTAACAC CGCGGGCATC GACATCTTCT CCAAGTTCTC AGCCTACATC 
AAAAACACCA AACAACAGAA CAATGCTGCC CTTGAGAGAG GCTTGACAAA GGCGCTGAGG 
AAGCTGGATG ACTACCTAAA CAGCCCTCTG CCAGAGGAGA TTGACACCAA CACCCACGGG 
GACGAGAAGG GGTCCCAGCG CAAGTTCCTG GATGGGGATG AGCTGACCCT GGCCGACTGC 
AATCTGCTGC CCAAGCTGCA TGTGGTCAAG ATTGTGGCTA AGAAGTACCG AAACTATGAC 
ATCCCAGCTG AGATGACAGG CTTGTGGCGA TACCTCAAGA ATGCCTATGC ACGGGACGAG 
TTCACCAACA CCTGTGCAGC TGACAGTGAG ATCGAGTTGG CCTACGCAGA TGTCGCCAGG 
CGCCTCAGCC GATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524198.1
CDS	227..1684
Translation	MNEDYSDTYDKIQKRSTDEAPDLQEESDAPLYDRVHEDSENDLYATRLESQEYEYLSPYVSKSDEATPASLEPEADGYLLPDEVFSESRAVRDVSMGDSHSPSQDPEPLSWEPQENGGATEEDVPSSHSLSQDPERLPWEPQENGGATEEDVPSSHSLSQDLERLPWEPQENGGATEEDVSSSHSLSQDLERLPWEPQENGGATEEDVPSLLSFRVQHSKVLATSQDSSISLSAAIGLGEKEAASSNPEIFLFVKAGIDGESIGNCPFSQRLFMILWLKGVVFNVTTVDLKRKPADLHNLAPGTHPPFLTFNGDVKTDVNKIEEFLEETLTPEKYPKLAAKHRESNTAGIDIFSKFSAYIKNTKQQNNAALERGLTKALRKLDDYLNSPLPEEIDTNTHGDEKGSQRKFLDGDELTLADCNLLPKLHVVKIVAKKYRNYDIPAEMTGLWRYLKNAYARDEFTNTCAADSEIELAYADVARRLSRS

Target ORF information:

RefSeq Version	XM_006524135.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chloride intracellular channel 5 (Clic5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524135.3

ORF Insert Sequence:

ATGAATGAAG ACTACAGTGA TACCTATGAT AAAATCCAAA AGAGGAGCAC AGATGAGGCT 
CCAGACCTGC AGGAGGAAAG TGACGCTCCT TTGTATGACA GAGTCCATGA AGACTCTGAA 
AATGATCTGT ATGCCACCAG GCTAGAAAGC CAGGAGTACG AATACTTGTC ACCGTATGTC 
TCGAAAAGTG ATGAAGCCAC CCCAGCCTCC CTGGAGCCAG AAGCAGATGG CTACCTCCTG 
CCGGATGAGG TGTTTTCAGA GTCCCGGGCG GTTCGGGATG TCTCCATGGG AGACTCACAT 
TCACCAAGCC AGGACCCAGA GCCTCTCTCC TGGGAACCTC AGGAGAATGG AGGAGCAACC 
GAGGAAGACG TGCCTTCCTC ACATTCACTA AGCCAGGACC CGGAGCGTCT CCCGTGGGAA 
CCTCAGGAGA ATGGAGGAGC AACCGAGGAA GATGTGCCTT CCTCACATTC ACTAAGCCAG 
GACCTGGAGC GTCTCCCGTG GGAACCTCAG GAGAATGGAG GAGCAACCGA GGAAGACGTG 
TCTTCCTCAC ATTCACTAAG CCAGGACCTG GAGCGTCTCC CGTGGGAACC TCAGGAGAAT 
GGAGGAGCAA CCGAGGAAGA CGTGCCTTCC TTACTGAGCT TCAGGGTTCA GCACAGCAAG 
GTGCTTGCCA CAAGCCAGGA TTCCTCTATC AGCCTTTCTG CTGCTATTGG GTTGGGAGAA 
AAAGAAGCAG CTTCCAGCAA CCCTGAGATC TTCCTGTTTG TGAAGGCTGG GATCGACGGG 
GAAAGCATTG GCAACTGTCC CTTCTCTCAG CGTCTCTTCA TGATCCTCTG GCTGAAAGGA 
GTCGTGTTCA ATGTCACCAC CGTGGATCTG AAAAGAAAGC CAGCCGATCT ACACAACCTG 
GCTCCTGGCA CGCACCCGCC CTTCCTGACC TTCAATGGGG ATGTGAAGAC AGATGTCAAC 
AAGATTGAAG AGTTCCTGGA GGAGACCCTA ACCCCCGAGA AGTACCCCAA ACTGGCTGCA 
AAACACCGGG AATCTAACAC CGCGGGCATC GACATCTTCT CCAAGTTCTC AGCCTACATC 
AAAAACACCA AACAACAGAA CAATGCTGCC CTTGAGAGAG GCTTGACAAA GGCGCTGAGG 
AAGCTGGATG ACTACCTAAA CAGCCCTCTG CCAGAGGAGA TTGACACCAA CACCCACGGG 
GACGAGAAGG GGTCCCAGCG CAAGTTCCTG GATGGGGATG AGCTGACCCT GGCCGACTGC 
AATCTGCTGC CCAAGCTGCA TGTGGTCAAG ATTGTGGCTA AGAAGTACCG AAACTATGAC 
ATCCCAGCTG AGATGACAGG CTTGTGGCGA TACCTCAAGA ATGCCTATGC ACGGGACGAG 
TTCACCAACA CCTGTGCAGC TGACAGTGAG ATCGAGTTGG CCTACGCAGA TGTCGCCAGG 
CGCCTCAGCC GATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.
Nature communications8(1)202(2017 08)
Tang T,Lang X,Xu C,Wang X,Gong T,Yang Y,Cui J,Bai L,Wang J,Jiang W,Zhou R

Both CLIC4 and CLIC5A activate ERM proteins in glomerular endothelium.
American journal of physiology. Renal physiology311(5)F945-F957(2016 Nov)
Tavasoli M,Al-Momany A,Wang X,Li L,Edwards JC,Ballermann BJ

Emerging biological roles of Cl- intracellular channel proteins.
Journal of cell science129(22)4165-4174(2016 11)
Argenzio E,Moolenaar WH

The chloride intracellular channel 5A stimulates podocyte Rac1, protecting against hypertension-induced glomerular injury.
Kidney international89(4)833-47(2016 Apr)
Tavasoli M,Li L,Al-Momany A,Zhu LF,Adam BA,Wang Z,Ballermann BJ

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Clic5 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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