Adgrf5 cDNA ORF clone, Mus musculus(house mouse)

The following Adgrf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adgrf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu53483 XM_006524124.4
Latest version!
Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu53483 XM_006524125.4
Latest version!
Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu53484 XM_006524126.4
Latest version!
Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu53485 XM_006524128.4
Latest version!
Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu53486 XM_006524129.3
Latest version!
Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu13458 NM_001081178.1
Latest version!
Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu53486 NM_001357332.1
Latest version!
Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu53485 XM_006524127.3
Latest version!
Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu53486 XM_017317437.1
Latest version!
Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OMu53486 XM_006524129.2 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu53485 XM_006524128.3 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu53483 XM_006524125.3 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu53483 XM_006524124.3 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu53484 XM_006524126.3 Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu53483
    Clone ID Related Accession (Same CDS sequence) XM_006524125.3 , XM_006524125.4 , XM_006524124.3 , XM_006524124.4
    Accession Version XM_006524125.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524125.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524188.1
    CDS293..4513
    Translation

    Target ORF information:

    RefSeq Version XM_006524125.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524125.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53483
    Clone ID Related Accession (Same CDS sequence) XM_006524125.3 , XM_006524125.4 , XM_006524124.3 , XM_006524124.4
    Accession Version XM_006524124.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524124.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524187.1
    CDS650..4870
    Translation

    Target ORF information:

    RefSeq Version XM_006524124.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524124.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53484
    Clone ID Related Accession (Same CDS sequence) XM_006524126.3 , XM_006524126.4
    Accession Version XM_006524126.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524126.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GCAGCCCCAA AGGCCCCCCA AGGCAATGAT GGAAATCTTG TTAGCAGTGG CAGTCCGTCA
    CCCCGATGGA ACATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524189.1
    CDS650..4867
    Translation

    Target ORF information:

    RefSeq Version XM_006524126.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524126.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GCAGCCCCAA AGGCCCCCCA AGGCAATGAT GGAAATCTTG TTAGCAGTGG CAGTCCGTCA
    CCCCGATGGA ACATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53485
    Clone ID Related Accession (Same CDS sequence) XM_006524128.4 , XM_006524128.3 , XM_006524127.3
    Accession Version XM_006524128.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524128.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG
    CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG
    GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC
    AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC
    ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA
    GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT
    GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC
    CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA
    GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA
    GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC
    ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA
    TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC
    TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC
    ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG
    TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC
    ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG
    CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC
    AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT
    GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT
    GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT
    AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA
    ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC
    ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG
    GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA
    ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT
    TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG
    TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT
    CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC
    TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT
    CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT
    ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG
    ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA
    TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC
    TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT
    ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA
    GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC
    GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC
    TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG
    GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG
    GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA
    ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC
    ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG
    AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC
    ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC
    CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA
    GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT
    TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC
    TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT
    TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT
    GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC
    AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC
    CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC
    GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG
    AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC
    TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC
    TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC
    AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT
    CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT
    CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG
    AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC
    GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG
    AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC
    AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA
    GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG
    GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG
    CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG
    TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT
    AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC
    TCTAGTGCTT ACTCACTGCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524191.1
    CDS292..4458
    Translation

    Target ORF information:

    RefSeq Version XM_006524128.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524128.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG
    CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG
    GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC
    AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC
    ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA
    GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT
    GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC
    CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA
    GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA
    GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC
    ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA
    TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC
    TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC
    ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG
    TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC
    ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG
    CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC
    AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT
    GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT
    GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT
    AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA
    ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC
    ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG
    GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA
    ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT
    TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG
    TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT
    CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC
    TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT
    CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT
    ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG
    ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA
    TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC
    TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT
    ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA
    GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC
    GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC
    TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG
    GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG
    GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA
    ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC
    ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG
    AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC
    ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC
    CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA
    GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT
    TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC
    TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT
    TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT
    GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC
    AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC
    CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC
    GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG
    AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC
    TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC
    TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC
    AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT
    CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT
    CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG
    AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC
    GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG
    AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC
    AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA
    GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG
    GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG
    CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG
    TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT
    AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC
    TCTAGTGCTT ACTCACTGCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53486
    Clone ID Related Accession (Same CDS sequence) XM_006524129.2 , XM_006524129.3 , XM_017317437.1 , NM_001357332.1
    Accession Version XM_006524129.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524192.1
    CDS650..4750
    Translation

    Target ORF information:

    RefSeq Version XM_006524129.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524129.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu13458
    Clone ID Related Accession (Same CDS sequence) NM_001081178.1
    Accession Version NM_001081178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-04
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC169504.1, CAAA01049588.1 and CAAA01202959.1. On Feb 6, 2007 this sequence version replaced XM_283438.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG
    CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG
    GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC
    AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC
    ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA
    GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT
    GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC
    CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA
    GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA
    GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC
    ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA
    TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC
    TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC
    ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG
    TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC
    ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG
    CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC
    AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT
    GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT
    GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT
    AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA
    ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC
    ATAGCTGTGA CCTTCACCTC TGTAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT
    TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG
    TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT
    CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC
    TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT
    CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT
    ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG
    ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA
    TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC
    TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT
    ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA
    GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC
    GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC
    TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG
    GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG
    GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA
    ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC
    ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG
    AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC
    ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC
    CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA
    GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT
    TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC
    TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT
    TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT
    GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC
    AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC
    CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC
    GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG
    AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC
    TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC
    TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC
    AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT
    CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT
    CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG
    AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC
    GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG
    AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC
    AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA
    GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG
    GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG
    CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG
    TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT
    AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC
    TCTAGTGCTT ACTCACTGCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001074647.1
    CDS267..4313
    Translation

    Target ORF information:

    RefSeq Version NM_001081178.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG
    CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG
    GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC
    AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC
    ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA
    GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT
    GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC
    CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA
    GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA
    GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC
    ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA
    TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC
    TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC
    ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG
    TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC
    ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG
    CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC
    AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT
    GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT
    GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT
    AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA
    ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC
    ATAGCTGTGA CCTTCACCTC TGTAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT
    TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG
    TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT
    CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC
    TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT
    CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT
    ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG
    ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA
    TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC
    TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT
    ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA
    GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC
    GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC
    TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG
    GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG
    GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA
    ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC
    ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG
    AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC
    ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC
    CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA
    GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT
    TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC
    TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT
    TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT
    GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC
    AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC
    CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC
    GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG
    AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC
    TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC
    TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC
    AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT
    CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT
    CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG
    AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC
    GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG
    AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC
    AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA
    GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG
    GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG
    CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG
    TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT
    AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC
    TCTAGTGCTT ACTCACTGCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53486
    Clone ID Related Accession (Same CDS sequence) XM_006524129.2 , XM_006524129.3 , XM_017317437.1 , NM_001357332.1
    Accession Version NM_001357332.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform 2 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT025700.16, AC169504.1, CT010585.12 and AW457181.1. On Oct 25, 2017 this sequence version replaced XM_017317437.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.213531.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001344261.1
    CDS191..4291
    Translation

    Target ORF information:

    RefSeq Version NM_001357332.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001357332.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53483
    Clone ID Related Accession (Same CDS sequence) XM_006524125.3 , XM_006524125.4 , XM_006524124.3 , XM_006524124.4
    Accession Version XM_006524125.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524125.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524188.1
    CDS295..4515
    Translation

    Target ORF information:

    RefSeq Version XM_006524125.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524125.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53483
    Clone ID Related Accession (Same CDS sequence) XM_006524125.3 , XM_006524125.4 , XM_006524124.3 , XM_006524124.4
    Accession Version XM_006524124.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524124.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524187.1
    CDS316..4536
    Translation

    Target ORF information:

    RefSeq Version XM_006524124.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524124.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53484
    Clone ID Related Accession (Same CDS sequence) XM_006524126.3 , XM_006524126.4
    Accession Version XM_006524126.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524126.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GCAGCCCCAA AGGCCCCCCA AGGCAATGAT GGAAATCTTG TTAGCAGTGG CAGTCCGTCA
    CCCCGATGGA ACATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524189.1
    CDS321..4538
    Translation

    Target ORF information:

    RefSeq Version XM_006524126.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524126.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGG AGATAATGCC AGCTACAAAG GGGAAGTGCC CGAGGTCCTA
    AGGCATGTGA TGGAAGTGAG TACAGTTCAG AGCACCACCG GGAACAGCCT AGAAACAAGT
    AAACCCCAGG AGCAGCCAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GCAGCCCCAA AGGCCCCCCA AGGCAATGAT GGAAATCTTG TTAGCAGTGG CAGTCCGTCA
    CCCCGATGGA ACATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53485
    Clone ID Related Accession (Same CDS sequence) XM_006524128.4 , XM_006524128.3 , XM_006524127.3
    Accession Version XM_006524128.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524128.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG
    CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG
    GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC
    AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC
    ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA
    GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT
    GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC
    CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA
    GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA
    GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC
    ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA
    TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC
    TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC
    ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG
    TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC
    ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG
    CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC
    AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT
    GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT
    GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT
    AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA
    ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC
    ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG
    GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA
    ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT
    TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG
    TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT
    CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC
    TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT
    CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT
    ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG
    ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA
    TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC
    TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT
    ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA
    GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC
    GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC
    TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG
    GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG
    GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA
    ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC
    ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG
    AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC
    ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC
    CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA
    GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT
    TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC
    TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT
    TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT
    GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC
    AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC
    CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC
    GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG
    AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC
    TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC
    TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC
    AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT
    CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT
    CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG
    AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC
    GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG
    AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC
    AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA
    GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG
    GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG
    CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG
    TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT
    AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC
    TCTAGTGCTT ACTCACTGCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524191.1
    CDS294..4460
    Translation

    Target ORF information:

    RefSeq Version XM_006524128.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524128.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG
    CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG
    GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC
    AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC
    ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA
    GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT
    GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC
    CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA
    GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA
    GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC
    ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA
    TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC
    TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC
    ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG
    TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC
    ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG
    CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC
    AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT
    GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT
    GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT
    AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA
    ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC
    ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG
    GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA
    ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT
    TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG
    TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT
    CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC
    TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT
    CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT
    ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG
    ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA
    TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC
    TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT
    ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA
    GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC
    GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC
    TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG
    GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG
    GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA
    ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC
    ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG
    AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC
    ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC
    CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA
    GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT
    TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC
    TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT
    TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT
    GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC
    AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC
    CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC
    GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG
    AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC
    TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC
    TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC
    AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT
    CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT
    CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG
    AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC
    GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG
    AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC
    AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA
    GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG
    GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG
    CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG
    TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT
    AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC
    TCTAGTGCTT ACTCACTGCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53485
    Clone ID Related Accession (Same CDS sequence) XM_006524128.4 , XM_006524128.3 , XM_006524127.3
    Accession Version XM_006524127.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4167bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524127.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG
    CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG
    GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC
    AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC
    ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA
    GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT
    GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC
    CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA
    GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA
    GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC
    ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA
    TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC
    TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC
    ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG
    TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC
    ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG
    CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC
    AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT
    GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT
    GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT
    AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA
    ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC
    ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG
    GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA
    ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT
    TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG
    TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT
    CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC
    TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT
    CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT
    ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG
    ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA
    TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC
    TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT
    ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA
    GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC
    GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC
    TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG
    GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG
    GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA
    ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC
    ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG
    AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC
    ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC
    CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA
    GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT
    TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC
    TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT
    TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT
    GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC
    AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC
    CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC
    GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG
    AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC
    TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC
    TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC
    AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT
    CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT
    CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG
    AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC
    GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG
    AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC
    AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA
    GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG
    GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG
    CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG
    TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT
    AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC
    TCTAGTGCTT ACTCACTGCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524190.1
    CDS548..4714
    Translation

    Target ORF information:

    RefSeq Version XM_006524127.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524127.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAATTCTCCA AGAACATGAG
    CTAGCCGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGCTG CAGCGGGTGA TCGCGTGGCG
    GAAGAATACA TGGTGGATAT TGAGATCAGC TTTGAAAATG TCTCCTTCCT GGAGTCCATC
    AGAGCTCACT TGAACAACCT CAGTTTTCCA ATTCGAGGGA CCGAAGCTGA CATTTTGAAC
    ATAGCAATGA CTACAGTCTG CACTCCTGCT GGAAATGACC TCTTGTGCTT CTGTGAGAAA
    GGCTACCAGT GGTCTGAGGA AAGGTGTCTC CACTCTCTCA CTTGTCAAGA CTATGACAGT
    GCCCTGCCAG GCGGCTACTG CAGTTGTCTG AAAGGACTTC CTCCCCAGGG ACCTTTCTGC
    CAGCTCCCAG AAGCATTCAT TACCTTAAAA CTCAAAGTCA GACTGAACAT AGGATTTCAA
    GAAGACCTAA AGAACACGTC TTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA
    GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC
    ACCAAGGGCA GCGTGGTTGT GAACTACGTA GTCCGGGTTA CATCGGCACC ACTGCCTGGA
    TCTATTCATA AAGCCAATGA ACAAGTCATA CAGAACCTCA ACCATACTTA CAAAATGGAC
    TACAACTCCT TCCAGGGAAC CCCGAGCAAT GAGACGAAAT TCACTGTCAT ACCAGAATTC
    ATCTTTGAGG GAGACAATGT GACTCTGGAG TGTGAAACTG AGTTCGTGAC CTCCAACACG
    TCTTGGTACT ATGGAGAAAA ACGCTCTGAC ATCCAGAACA GCGACAAATA CTCCATTCAC
    ACCACTGTCA TCAACAATAT AAGCCTGATC ACCCGCCTTA CCATTTACAA CTTCACACAG
    CATGATGCGG GCATGTATGG TTGCAATGTG ACATTGGATA TTTTTGAATA TGGAACAGTC
    AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AGGAGAGAAA GGTGGTGTGT
    GACAACCATC CTATATCGCT GAACTGCTGC AGCGAGAATA TTGCAAACTG GAGCAGTATT
    GAATGGAAAC AGGAAGGGAA GATAAGCATT CTAGGGAACC CAGAATCAGA CCTAGAGTCT
    AGCTGCAGCA CTTACACGCT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG GTCTTCTGGA
    ACAACAGTCA TCTACACATG CGAGTTCGTC AGTGCCTATG GAGCCAGAGG CAGTAAGAAC
    ATAGCTGTGA CCTTCACCTC TGTAGGAGAT AATGCCAGCT ACAAAGGGGA AGTGCCCGAG
    GTCCTAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGCCTAGAA
    ACAAGTAAAC CCCAGGAGCA GCCAGCCAAC CTAACAATAA CCCGGGACCC AATTTCTGTT
    TCTGAGGGAC AAAGCTTTTC TATAACCTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG
    TACTGGAACA CCTCAGCTGG TATTAAAATC CACCCAAGGT TTTATACCAT GAGGAGGTAT
    CAGGATGGAG CAGAGTCGGT GCTGATGGTG AAGACCTCCA CCCGAGAGTG GAACGGAACC
    TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATTG CCACCAAAGA TGTCACCGTT
    CACCCGCTGC CTCTGGTGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGT
    ACCAGTTCCC ATCAATTCAA ATGCTGCGTA GAAGAGGATG CAGGGGAAGA GTACGCTGTG
    ACTTTCCACG TGGACTCCTC ATCCTTTCCC GCTGAAAGAG AAGTTATCGG CAAGCAAGCA
    TGCTACACAT ACAGTCTCCC AGCGAACTTA CCTCGATGTC CCAAAGACAT CGCAGTGTTC
    TGCCACTTCA CCAATGCAGC CAACAGCTCA GTCCGGAGCC CATCTATGAA GCTCAAACTT
    ATTCCAAGGG AGAATGTCAC ATGCCAGGAT CCCATCATTG GCATTGGGGA TCCTGGGAAA
    GTCATTCAGA AGCTGTGCCA GTTCTCAGGT GTTTACGGAA GCCCTGGACA GGCCATCGGC
    GGGACTGTCA CTTACAAATG TGTGGGCACC CAGTGGAAGG AGGAGTCAAG GGCCTGCATC
    TCAGCTCCAA TCAATGGTTT GCTCCAGGTG GCCAAGGCTT TGATCAAGAG CCCCACCCAG
    GACCAGAAGC TCCCTACATA CCTGAGGGAC CTTTCTGTTA GTGCTGGGAA GGAAGAACAG
    GACATCCGCT CATCTCCAGG AAGCCTGGGA GCCATCATCA GCATCCTTGA CTTGCTCTCA
    ACGGTTCCCA CCCAAGTGAA TTCAGAAATG ATGAGAGACA TACTTGCTAC CATTAACGTC
    ATCTTAGACA AGTCTGCCTT GAACTCCTGG GAGAAGTTGC TACAGCAACA GAGCAATCAG
    AGTTCTCAGT TCCTGCACTC CGTGGAAAGG TTTTCCCAAG CCCTCCAGCT GGGAGACAGC
    ACTCCTCCAT TCCTCGCCCA CCCTAACGTT CAGATGAAGA GCATGGTGAT AAAACGTGGC
    CACCCCCAAA TCTACCAACA GCAGTTTATC TTCAAAGACT CTGACCTGTG GGGTGATGTA
    GCCATTGATG AGTGTCAGCT GGGAAACTTG CAGCCTGATT CATCGATCGT TACTGTGGCT
    TTCCCAACTC TCAAAGCCAT CCTTGCCCAG GATGTACAGA GAAAGACATC CTCCAACAGC
    TTAGTAATGA CAACCACTGT CAGCCATAAC ATTGTCAAGC CATTTAGGAT TTCTATGACT
    TTTAAGAACA ACCATCGTTC GGGTGGCAAG CCACAGTGTG TCTTCTGGAA CTTCAGTCTT
    GCCAACAATA CAGGGGGCTG GGACAGCAGT GGGTGCTCTG TGGAAGATGA TGGTAGGGAC
    AATAGGGACA GAGTCTTCTG CAAGTGTAAC CACCTGACGT CATTCTCCAT CCTCATGTCC
    CCAGACTCCC CAGATCCAGG GTCTCTTTTA AAAATACTTC TGGATATCAT TTCTTACATC
    GGGTTGGGGT TTTCCATAGT CAGCTTAGCT GCCTGTCTAG TTGTGGAAGC CATGGTGTGG
    AAATCTGTGA CCAAGAACCG AACTTCCTAT ATGCGCCACA TCTGCATAGT CAACATTGCC
    TTTTGCCTTC TGATTGCTGA CATCTGGTTC ATTGTGGCTG GTGCTATCCA CGACGGTCGC
    TACCCACTCA ACGAAACAGC CTGTGTGGCC GCCACATTCT TCATTCACTT CTTCTACCTC
    AGTGTCTTCT TCTGGATGCT AACTCTAGGC CTCATGCTCT TCTACCGGCT GATTTTCATT
    CTACACGATG CAAGCAAGTC CACTCAGAAA GCCATCGCAT TTTCTCTAGG CTATGGCTGT
    CCCCTCATTA TCTCCTCTAT CACAGTGGGG GTTACGCAGC CACAGGAAGT CTACATGAGG
    AAGAACGCGT GTTGGCTCAA CTGGGAGGAC ACCAGAGCAC TGCTGGCTTT TGCCATCCCC
    GCGTTGATTA TTGTGGTGGT AAATGTGAGC ATCACAGTTG TGGTCATCAC CAAGATCCTG
    AGGCCCTCCA TTGGGGACAA GCCAGGCAAG CAAGAGAAGA GCAGCCTATT CCAGATCAGC
    AAGAGTATCG GGGTCCTCAC ACCACTCTTG GGGCTCACTT GGGGTTTCGG TCTTGCCACA
    GTGATCCAGG GAAGCAATGC TGTGTTCCAC ATCATATTTA CTCTCCTCAA TGCCTTCCAG
    GGGCTCTTCA TTTTGCTCTT TGGCTGCCTC TGGGATCAGA AGGTGCAGGA AGCTTTGCTG
    CATAAGTTTT CATTGTCAAG GTGGTCTTCT CAACACTCAA AGTCAACATC CATAGGTTCG
    TCAACACCCG TGTTTTCAAT GAGTTCTCCA ATATCCCGAA GATTTAATAA TCTTTTTGGT
    AAAACAGGAA CATATAACGT TTCCACCCCA GAGACAACCA GTTCATCCCT GGAAAATTCC
    TCTAGTGCTT ACTCACTGCT CAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53486
    Clone ID Related Accession (Same CDS sequence) XM_006524129.2 , XM_006524129.3 , XM_017317437.1 , NM_001357332.1
    Accession Version XM_006524129.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product adhesion G protein-coupled receptor F5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006524192.1
    CDS315..4415
    Translation

    Target ORF information:

    RefSeq Version XM_006524129.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006524129.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu53486
    Clone ID Related Accession (Same CDS sequence) XM_006524129.2 , XM_006524129.3 , XM_017317437.1 , NM_001357332.1
    Accession Version XM_017317437.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product adhesion G protein-coupled receptor F5 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172926.1
    CDS191..4291
    Translation

    Target ORF information:

    RefSeq Version XM_017317437.1
    Organism Mus musculus(House mouse)
    Definition Mus musculus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017317437.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGATCGC CAAGGACCTT CACGTTCTAC TTCCTGCTTC TTGTGATTTG TTCTTCAGAA 
    GCTGCGCTGA GCACACCAAC AGAGCCCATT GTTCAACCTT CAGTGAGTAA AGCTAAACCT
    GCAGTCATTT CCTCCGCCCA GGTCTTTGAT TTCCCGATTC TCCAAGAACA TGAGCTAGCC
    GGGGAAGAGC TACTGAGGCC AAAAAGAGCA GCTGCAGCGG GTGATCGCGT GGCGGAAGAA
    TACATGGTGG ATATTGAGAT CAGCTTTGAA AATGTCTCCT TCCTGGAGTC CATCAGAGCT
    CACTTGAACA ACCTCAGTTT TCCAATTCGA GGGACCGAAG CTGACATTTT GAACATAGCA
    ATGACTACAG TCTGCACTCC TGCTGGAAAT GACCTCTTGT GCTTCTGTGA GAAAGGCTAC
    CAGTGGTCTG AGGAAAGGTG TCTCCACTCT CTCACTTGTC AAGACTATGA CAGTGCCCTG
    CCAGGCGGCT ACTGCAGTTG TCTGAAAGGA CTTCCTCCCC AGGGACCTTT CTGCCAGCTC
    CCAGAAGCAT TCATTACCTT AAAACTCAAA GTCAGACTGA ACATAGGATT TCAAGAAGAC
    CTAAAGAACA CGTCTTCTGC CCTCTATAGG TCCTACAAGA CTGATCTGGA AAGAGCGTTC
    CGAGCGGGTT ACAGAACTTT ACCAGGATTC AGATCGGTGA CTGTGACACA GTTCACCAAG
    GGCAGCGTGG TTGTGAACTA CGTAGTCCGG GTTACATCGG CACCACTGCC TGGATCTATT
    CATAAAGCCA ATGAACAAGT CATACAGAAC CTCAACCATA CTTACAAAAT GGACTACAAC
    TCCTTCCAGG GAACCCCGAG CAATGAGACG AAATTCACTG TCATACCAGA ATTCATCTTT
    GAGGGAGACA ATGTGACTCT GGAGTGTGAA ACTGAGTTCG TGACCTCCAA CACGTCTTGG
    TACTATGGAG AAAAACGCTC TGACATCCAG AACAGCGACA AATACTCCAT TCACACCACT
    GTCATCAACA ATATAAGCCT GATCACCCGC CTTACCATTT ACAACTTCAC ACAGCATGAT
    GCGGGCATGT ATGGTTGCAA TGTGACATTG GATATTTTTG AATATGGAAC AGTCAGAAAA
    TTAGATGTCA CTCCCATCCG GATTTTGGCC AAGGAGGAGA GAAAGGTGGT GTGTGACAAC
    CATCCTATAT CGCTGAACTG CTGCAGCGAG AATATTGCAA ACTGGAGCAG TATTGAATGG
    AAACAGGAAG GGAAGATAAG CATTCTAGGG AACCCAGAAT CAGACCTAGA GTCTAGCTGC
    AGCACTTACA CGCTCAAGGC AGATGGAACC CAGTGTCCCA GTGGGTCTTC TGGAACAACA
    GTCATCTACA CATGCGAGTT CGTCAGTGCC TATGGAGCCA GAGGCAGTAA GAACATAGCT
    GTGACCTTCA CCTCTGTAGC CAACCTAACA ATAACCCGGG ACCCAATTTC TGTTTCTGAG
    GGACAAAGCT TTTCTATAAC CTGCCTCAGC GATGTGAGTA GCTTTGATGA GGTGTACTGG
    AACACCTCAG CTGGTATTAA AATCCACCCA AGGTTTTATA CCATGAGGAG GTATCAGGAT
    GGAGCAGAGT CGGTGCTGAT GGTGAAGACC TCCACCCGAG AGTGGAACGG AACCTACCAC
    TGCATATTTA GATATAAGAA TTCATACAGC ATTGCCACCA AAGATGTCAC CGTTCACCCG
    CTGCCTCTGG TGTCAGACAT CATGATGGAT CCTTTGGAAG CCAGTGGTCT GTGTACCAGT
    TCCCATCAAT TCAAATGCTG CGTAGAAGAG GATGCAGGGG AAGAGTACGC TGTGACTTTC
    CACGTGGACT CCTCATCCTT TCCCGCTGAA AGAGAAGTTA TCGGCAAGCA AGCATGCTAC
    ACATACAGTC TCCCAGCGAA CTTACCTCGA TGTCCCAAAG ACATCGCAGT GTTCTGCCAC
    TTCACCAATG CAGCCAACAG CTCAGTCCGG AGCCCATCTA TGAAGCTCAA ACTTATTCCA
    AGGGAGAATG TCACATGCCA GGATCCCATC ATTGGCATTG GGGATCCTGG GAAAGTCATT
    CAGAAGCTGT GCCAGTTCTC AGGTGTTTAC GGAAGCCCTG GACAGGCCAT CGGCGGGACT
    GTCACTTACA AATGTGTGGG CACCCAGTGG AAGGAGGAGT CAAGGGCCTG CATCTCAGCT
    CCAATCAATG GTTTGCTCCA GGTGGCCAAG GCTTTGATCA AGAGCCCCAC CCAGGACCAG
    AAGCTCCCTA CATACCTGAG GGACCTTTCT GTTAGTGCTG GGAAGGAAGA ACAGGACATC
    CGCTCATCTC CAGGAAGCCT GGGAGCCATC ATCAGCATCC TTGACTTGCT CTCAACGGTT
    CCCACCCAAG TGAATTCAGA AATGATGAGA GACATACTTG CTACCATTAA CGTCATCTTA
    GACAAGTCTG CCTTGAACTC CTGGGAGAAG TTGCTACAGC AACAGAGCAA TCAGAGTTCT
    CAGTTCCTGC ACTCCGTGGA AAGGTTTTCC CAAGCCCTCC AGCTGGGAGA CAGCACTCCT
    CCATTCCTCG CCCACCCTAA CGTTCAGATG AAGAGCATGG TGATAAAACG TGGCCACCCC
    CAAATCTACC AACAGCAGTT TATCTTCAAA GACTCTGACC TGTGGGGTGA TGTAGCCATT
    GATGAGTGTC AGCTGGGAAA CTTGCAGCCT GATTCATCGA TCGTTACTGT GGCTTTCCCA
    ACTCTCAAAG CCATCCTTGC CCAGGATGTA CAGAGAAAGA CATCCTCCAA CAGCTTAGTA
    ATGACAACCA CTGTCAGCCA TAACATTGTC AAGCCATTTA GGATTTCTAT GACTTTTAAG
    AACAACCATC GTTCGGGTGG CAAGCCACAG TGTGTCTTCT GGAACTTCAG TCTTGCCAAC
    AATACAGGGG GCTGGGACAG CAGTGGGTGC TCTGTGGAAG ATGATGGTAG GGACAATAGG
    GACAGAGTCT TCTGCAAGTG TAACCACCTG ACGTCATTCT CCATCCTCAT GTCCCCAGAC
    TCCCCAGATC CAGGGTCTCT TTTAAAAATA CTTCTGGATA TCATTTCTTA CATCGGGTTG
    GGGTTTTCCA TAGTCAGCTT AGCTGCCTGT CTAGTTGTGG AAGCCATGGT GTGGAAATCT
    GTGACCAAGA ACCGAACTTC CTATATGCGC CACATCTGCA TAGTCAACAT TGCCTTTTGC
    CTTCTGATTG CTGACATCTG GTTCATTGTG GCTGGTGCTA TCCACGACGG TCGCTACCCA
    CTCAACGAAA CAGCCTGTGT GGCCGCCACA TTCTTCATTC ACTTCTTCTA CCTCAGTGTC
    TTCTTCTGGA TGCTAACTCT AGGCCTCATG CTCTTCTACC GGCTGATTTT CATTCTACAC
    GATGCAAGCA AGTCCACTCA GAAAGCCATC GCATTTTCTC TAGGCTATGG CTGTCCCCTC
    ATTATCTCCT CTATCACAGT GGGGGTTACG CAGCCACAGG AAGTCTACAT GAGGAAGAAC
    GCGTGTTGGC TCAACTGGGA GGACACCAGA GCACTGCTGG CTTTTGCCAT CCCCGCGTTG
    ATTATTGTGG TGGTAAATGT GAGCATCACA GTTGTGGTCA TCACCAAGAT CCTGAGGCCC
    TCCATTGGGG ACAAGCCAGG CAAGCAAGAG AAGAGCAGCC TATTCCAGAT CAGCAAGAGT
    ATCGGGGTCC TCACACCACT CTTGGGGCTC ACTTGGGGTT TCGGTCTTGC CACAGTGATC
    CAGGGAAGCA ATGCTGTGTT CCACATCATA TTTACTCTCC TCAATGCCTT CCAGGGGCTC
    TTCATTTTGC TCTTTGGCTG CCTCTGGGAT CAGAAGGTGC AGGAAGCTTT GCTGCATAAG
    TTTTCATTGT CAAGGTGGTC TTCTCAACAC TCAAAGTCAA CATCCATAGG TTCGTCAACA
    CCCGTGTTTT CAATGAGTTC TCCAATATCC CGAAGATTTA ATAATCTTTT TGGTAAAACA
    GGAACATATA ACGTTTCCAC CCCAGAGACA ACCAGTTCAT CCCTGGAAAA TTCCTCTAGT
    GCTTACTCAC TGCTCAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.