Adgrf5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adgrf5
Entrez Gene ID	245977
Full Name	adhesion G protein-coupled receptor F5
Synonyms	Gpr116,Gprhep,Ig-Hepta
General protein information	Preferred Names adhesion G protein-coupled receptor F5 Names adhesion G protein-coupled receptor F5 G protein-coupled hepta-helical receptor Ig-Hepta G-protein coupled hepta-helical receptor Ig-hepta G-protein coupled receptor 116 probable G-protein coupled receptor 116
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q13
Summary	a G protein-coupled receptor; may have a role in the regulation of acid-base balance [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008766835.2	XP_008765057.1	adhesion G protein-coupled receptor F5 isoform X4
XM_008766832.2	XP_008765054.1	adhesion G protein-coupled receptor F5 isoform X1
XM_008766833.2	XP_008765055.1	adhesion G protein-coupled receptor F5 isoform X1
XM_008766834.2	XP_008765056.1	adhesion G protein-coupled receptor F5 isoform X2
XM_006244594.3	XP_006244656.1	adhesion G protein-coupled receptor F5 isoform X3
NM_139110.1	NP_620810.1	adhesion G protein-coupled receptor F5 precursor
XM_006244597.3	XP_006244659.1	adhesion G protein-coupled receptor F5 isoform X5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPR116	XP_001140861.2
Gallus gallus (chicken)	GPR116	XP_004935990.1
Xenopus tropicalis (tropical clawed frog)	gpr116	NP_001107298.1
Mus musculus (house mouse)	Gpr116	NP_001074647.1
Homo sapiens (human)	GPR116	NP_001091988.1
	GPR116	XP_001103527.2
Canis lupus familiaris (dog)	GPR116	XP_005627499.1
Bos taurus (cattle)	GPR116	NP_001180172.1
Rattus norvegicus (Norway rat)	Gpr116	NP_620810.1

Gene Ontology
Bibliography

Related articles in PubMed

Developmental vascular remodeling defects and postnatal kidney failure in mice lacking Gpr116 (Adgrf5) and Eltd1 (Adgrl4).
Lu S, Liu S, Wietelmann A, Kojonazarov B, Atzberger A, Tang C, Schermuly RT, Gr?ne HJ, Offermanns S
PloS one12(8)e0183166(2017)

Targeted Disruption of Ig-Hepta/Gpr116 Causes Emphysema-like Symptoms That Are Associated with Alveolar Macrophage Activation.
Ariestanti DM, Ando H, Hirose S, Nakamura N
The Journal of biological chemistry290(17)11032-40(2015 Apr)

Orphan G protein-coupled receptor GPR116 regulates pulmonary surfactant pool size.
Bridges JP, Ludwig MG, Mueller M, Kinzel B, Sato A, Xu Y, Whitsett JA, Ikegami M
American journal of respiratory cell and molecular biology49(3)348-57(2013 Sep)

Essential regulation of lung surfactant homeostasis by the orphan G protein-coupled receptor GPR116.
Yang MY, Hilton MB, Seaman S, Haines DC, Nagashima K, Burks CM, Tessarollo L, Ivanova PT, Brown HA, Umstead TM, Floros J, Chroneos ZC, St Croix B
Cell reports3(5)1457-64(2013 May)

Adipose tissue deletion of Gpr116 impairs insulin sensitivity through modulation of adipose function.
Nie T, Hui X, Gao X, Li K, Lin W, Xiang X, Ding M, Kuang Y, Xu A, Fei J, Wang Z, Wu D
FEBS letters586(20)3618-25(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Adgrf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adgrf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02200	NM_139110.1 Latest version!	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$993.30 $1419.00
ORa32039	XM_006244594.3 Latest version!	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa32041	XM_006244597.3 Latest version!	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa44402	XM_008766832.2 Latest version!	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa44402	XM_008766833.2 Latest version!	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa44403	XM_008766834.2 Latest version!	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa44404	XM_008766835.2 Latest version!	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adgrf5 ( NM_139110.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620810.1 Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), mRNA.

Price & Availability↑

CloneID	ORa02200
Clone ID Related Accession (Same CDS sequence)	NM_139110.1
Accession Version	NM_139110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	adhesion G protein-coupled receptor F5 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB019120.1. ##Evidence-Data-START## Transcript exon combination :: AB019120.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGGACCC CAGAAACAGA CCTAGAGTCT 
AGCTGCAGCA CTTATACACT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG TTCTTCTGGA 
ACAACAGTCA TCTACACGTG TGAGTTCGTC AGCGTCTACG GAGCCAAAGG CAGTAAGAAC 
ATAGCCGTGA CCTTCACCTC TGTAGCCAAC CTAACAATAA CCCCGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACTTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CTTCCGCTGG TATTAAAATC CATCCAAGGT TTTATACCAT GAGGAGGTAT 
CGGGATGGAG CAGAGTCGGT GCTGACGGTG AAGACCTCCA CCAGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATAG CCACCAAAGA TGTCACTGTT 
CACCCTCTGC CTCTGGAGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGC 
ACCAGTTCCC ATCAGTTCAA GTGCTGCATA GAAGAGAACG ATGGGGAAGA GTACATTGTG 
ACTTTCCACG TGGATTCCTC ATCCTTTCCT GCTGAAAGAG AAGTTATTGG CAAGCAGGCA 
TGCTACACAT ACAGTCTCCC AGGAAAGTTA CCATCACGGT GTCCCAAAGA CATTGACGTG 
TTCTGCCACT TTACCAATGC AGCCAACAGC TCAGTCCGGA GCCCATCTAT GAAGCTTACC 
CTTGTTCCAG GGAAGAATAT CACATGCCAG GATCCCATCA TAGGTATTGG GGAACCTGGG 
AAAGTCATCC AGAAGCTGTG CCAGTTTGCA GGTGTTTCTA GAAGCCCTGG ACAGACCATT 
GGTGGAACCG TCACTTATAA ATGTGTAGGC TCCCAGTGGA AGGAGGAGAC AAGGGCCTGC 
ATATCAGCCC CAATCAATGG TTTGCTCCAG TTGGCCAAGG CTTTGATCAA GAGCCCCTCC 
CAGGATCAGA AGCTCCCTAA ATACCTGCGG GACCTTTCTG TTAGCACTGG GAAGGAAGAA 
CAGGATATAC GCTCATCGCC CGGGAGCCTG GGAGCCATCA TCAGCATCCT TGATTTGCTT 
TCAACAGTTC CCACTCAAGT GAATTCAGAA ATGATGAGGG ATATACTTGC TACCATTAAT 
GTCATCTTAG ACAAGTCCAC CTTGAACTCC TGGGAGAAGT TACTACAGCA ACAGAGCAAC 
CAGAGCTCTC AGTTCCTGCA GTCAGTGGAG AGGTTTTCCA AAGCGCTCGA GCTGGGAGAC 
AGCACCCCTC CCTTCCTCTT CCACCCTAAT GTTCAGATGA AGAGCATGGT GATAAAACGT 
GGCCACGCCC AAATGTACCA ACAGAAATTT GTCTTCACAG ACTCTGACCT ATGGGGTGAT 
GTAGCCATTG ATGAGTGTCA GCTGGGAAGC TTGCAGCCTG ATTCATCGAT CGTTACTGTG 
GCTTTCCCAA CTCTCAAAGC CATCCTGGCC CAGGATGGAC AGAGAAAGAC ACCCTCCAAC 
AGCCTAGTAA TGACCACCAC TGTCAGCCAT AATATCGTCA AGCCTTTTAG GATTTCTATG 
ACATTCAAGA ACAACCATCG TTCGGGTGGC AAGCCACAGT GTGTCTTCTG GAACTTCAGC 
CTTGCCAATA ATACAGGGGG CTGGGATAGC AGTGGGTGCA CTGTGGAAGA TGATGGTAGG 
GACAATAGGG ACAGAGTCTT CTGCAAGTGT AACCACCTGA CGTCATTCTC CATTCTCATG 
TCCCCAGACT CCCCAGACCC AGGATCTCTT TTAAAAATAC TTCTGGATAT CATTTCTTAC 
ATCGGGTTGG GCTTTTCCAT AGTCAGCTTA GCTGCCTGTC TAGTTGTGGA AGCCATGGTG 
TGGAAATCAG TGACCAAGAA CCGAACTTCC TATATGCGCC ACATCTGCAT CGTCAACATT 
GCCCTTTGCC TTCTGATTGC TGACATCTGG TTCATTGTGG CTGGTGCTAT CCACGATGGG 
CATTACCCAC TCAACGAAAC AGCCTGTGTG GCCGCCACAT TCTTCATTCA CTTCTTCTAC 
CTCAGTGTCT TCTTCTGGAT GCTAACTCTG GGCCTCATGC TCTTCTACCG GCTGATTTTC 
ATTCTACATG ACGCGAGCAA GTCCACGCAG AAAGCCATTG CCTTTTCTCT AGGCTATGGC 
TGTCCACTCA TTATCTCATC CATCACAGTG GGGGTTACAC AGCCACAGGA AGTTTACATG 
AGGAAGAATG CATGTTGGCT CAACTGGGAG GACACCAGAG CACTGCTGGC TTTTGCTATC 
CCAGCGTTGA TTATTGTGGT GGTGAACGTG AGCATCACAG TTGTGGTCAT CACCAAGATC 
CTAAGGCCCT CCGTCGGAGA CAAGCCAGGC AAGCAGGAAA AGAGCAGCCT ATTCCAGATC 
AGCAAGAGCA TTGGAGTCCT CACGCCACTC TTGGGGCTCA CTTGGGGTTT TGGTCTGGCC 
ACAGTGATCC AGGGGAGCAA TGCTGTGTTC CACATCATAT TTACTCTCCT CAATGCCTTT 
CAGGGGCTCT TCATTTTGCT CTTTGGCTGC CTCTGGGATC AGAAGGTACA GGAAGCTTTG 
CTGCATAAGT TTTCATTGTC AAGGTGGTCT TCTCAACACT CAAAGTCAAC ATCCTTAGGT 
TCATCAACAC CTGTGTTTTC GATGAGTTCT CCGATATCCC GAAGATTTAA TAATCTTTTT 
GGTAAAACAG GAACATATAA CGTTTCCACC CCAGAGACAA CCAGCTCATC CGTGGAAAAT 
TCCTCTAGTG CTTACTCATT GCTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620810.1
CDS	179..4228
Translation	MKSSRTVTLYFVLIVICSSEATWSRPAEPIVHPLILQEHELAGEELLRPKRAVAVGGPVAEEYTVDVEISFENVSFLESIRAHLNSLRFPVQGNGTDILSMAMTTVCTPTGNDLLCFCEKGYQWPEERCLSSLTCQEHDSALPGRYCNCLKGLPPQGPFCQLPETYITLKIKVRLNIGFQEDLENTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVDYIVEVASAPLPGSIHKANEQVIQNLNQTYKMDYNSFQGTPSNETKFTVTPEFIFEGDNVTLECESEFVSSNTSWFYGEKRSDIQNSDKFSIHTSIINNISLVTRLTIFNFTQHDAGLYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNNPISLNCCSENIANWSRIEWKQEGKINIEGTPETDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSVYGAKGSKNIAVTFTSVANLTITPDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYRDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLESDIMMDPLEASGLCTSSHQFKCCIEENDGEEYIVTFHVDSSSFPAEREVIGKQACYTYSLPGKLPSRCPKDIDVFCHFTNAANSSVRSPSMKLTLVPGKNITCQDPIIGIGEPGKVIQKLCQFAGVSRSPGQTIGGTVTYKCVGSQWKEETRACISAPINGLLQLAKALIKSPSQDQKLPKYLRDLSVSTGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSTLNSWEKLLQQQSNQSSQFLQSVERFSKALELGDSTPPFLFHPNVQMKSMVIKRGHAQMYQQKFVFTDSDLWGDVAIDECQLGSLQPDSSIVTVAFPTLKAILAQDGQRKTPSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCTVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIALCLLIADIWFIVAGAIHDGHYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSVGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSVENSSSAYSLLN

Target ORF information:

RefSeq Version	NM_139110.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139110.1

ORF Insert Sequence:

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGGACCC CAGAAACAGA CCTAGAGTCT 
AGCTGCAGCA CTTATACACT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG TTCTTCTGGA 
ACAACAGTCA TCTACACGTG TGAGTTCGTC AGCGTCTACG GAGCCAAAGG CAGTAAGAAC 
ATAGCCGTGA CCTTCACCTC TGTAGCCAAC CTAACAATAA CCCCGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACTTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CTTCCGCTGG TATTAAAATC CATCCAAGGT TTTATACCAT GAGGAGGTAT 
CGGGATGGAG CAGAGTCGGT GCTGACGGTG AAGACCTCCA CCAGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATAG CCACCAAAGA TGTCACTGTT 
CACCCTCTGC CTCTGGAGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGC 
ACCAGTTCCC ATCAGTTCAA GTGCTGCATA GAAGAGAACG ATGGGGAAGA GTACATTGTG 
ACTTTCCACG TGGATTCCTC ATCCTTTCCT GCTGAAAGAG AAGTTATTGG CAAGCAGGCA 
TGCTACACAT ACAGTCTCCC AGGAAAGTTA CCATCACGGT GTCCCAAAGA CATTGACGTG 
TTCTGCCACT TTACCAATGC AGCCAACAGC TCAGTCCGGA GCCCATCTAT GAAGCTTACC 
CTTGTTCCAG GGAAGAATAT CACATGCCAG GATCCCATCA TAGGTATTGG GGAACCTGGG 
AAAGTCATCC AGAAGCTGTG CCAGTTTGCA GGTGTTTCTA GAAGCCCTGG ACAGACCATT 
GGTGGAACCG TCACTTATAA ATGTGTAGGC TCCCAGTGGA AGGAGGAGAC AAGGGCCTGC 
ATATCAGCCC CAATCAATGG TTTGCTCCAG TTGGCCAAGG CTTTGATCAA GAGCCCCTCC 
CAGGATCAGA AGCTCCCTAA ATACCTGCGG GACCTTTCTG TTAGCACTGG GAAGGAAGAA 
CAGGATATAC GCTCATCGCC CGGGAGCCTG GGAGCCATCA TCAGCATCCT TGATTTGCTT 
TCAACAGTTC CCACTCAAGT GAATTCAGAA ATGATGAGGG ATATACTTGC TACCATTAAT 
GTCATCTTAG ACAAGTCCAC CTTGAACTCC TGGGAGAAGT TACTACAGCA ACAGAGCAAC 
CAGAGCTCTC AGTTCCTGCA GTCAGTGGAG AGGTTTTCCA AAGCGCTCGA GCTGGGAGAC 
AGCACCCCTC CCTTCCTCTT CCACCCTAAT GTTCAGATGA AGAGCATGGT GATAAAACGT 
GGCCACGCCC AAATGTACCA ACAGAAATTT GTCTTCACAG ACTCTGACCT ATGGGGTGAT 
GTAGCCATTG ATGAGTGTCA GCTGGGAAGC TTGCAGCCTG ATTCATCGAT CGTTACTGTG 
GCTTTCCCAA CTCTCAAAGC CATCCTGGCC CAGGATGGAC AGAGAAAGAC ACCCTCCAAC 
AGCCTAGTAA TGACCACCAC TGTCAGCCAT AATATCGTCA AGCCTTTTAG GATTTCTATG 
ACATTCAAGA ACAACCATCG TTCGGGTGGC AAGCCACAGT GTGTCTTCTG GAACTTCAGC 
CTTGCCAATA ATACAGGGGG CTGGGATAGC AGTGGGTGCA CTGTGGAAGA TGATGGTAGG 
GACAATAGGG ACAGAGTCTT CTGCAAGTGT AACCACCTGA CGTCATTCTC CATTCTCATG 
TCCCCAGACT CCCCAGACCC AGGATCTCTT TTAAAAATAC TTCTGGATAT CATTTCTTAC 
ATCGGGTTGG GCTTTTCCAT AGTCAGCTTA GCTGCCTGTC TAGTTGTGGA AGCCATGGTG 
TGGAAATCAG TGACCAAGAA CCGAACTTCC TATATGCGCC ACATCTGCAT CGTCAACATT 
GCCCTTTGCC TTCTGATTGC TGACATCTGG TTCATTGTGG CTGGTGCTAT CCACGATGGG 
CATTACCCAC TCAACGAAAC AGCCTGTGTG GCCGCCACAT TCTTCATTCA CTTCTTCTAC 
CTCAGTGTCT TCTTCTGGAT GCTAACTCTG GGCCTCATGC TCTTCTACCG GCTGATTTTC 
ATTCTACATG ACGCGAGCAA GTCCACGCAG AAAGCCATTG CCTTTTCTCT AGGCTATGGC 
TGTCCACTCA TTATCTCATC CATCACAGTG GGGGTTACAC AGCCACAGGA AGTTTACATG 
AGGAAGAATG CATGTTGGCT CAACTGGGAG GACACCAGAG CACTGCTGGC TTTTGCTATC 
CCAGCGTTGA TTATTGTGGT GGTGAACGTG AGCATCACAG TTGTGGTCAT CACCAAGATC 
CTAAGGCCCT CCGTCGGAGA CAAGCCAGGC AAGCAGGAAA AGAGCAGCCT ATTCCAGATC 
AGCAAGAGCA TTGGAGTCCT CACGCCACTC TTGGGGCTCA CTTGGGGTTT TGGTCTGGCC 
ACAGTGATCC AGGGGAGCAA TGCTGTGTTC CACATCATAT TTACTCTCCT CAATGCCTTT 
CAGGGGCTCT TCATTTTGCT CTTTGGCTGC CTCTGGGATC AGAAGGTACA GGAAGCTTTG 
CTGCATAAGT TTTCATTGTC AAGGTGGTCT TCTCAACACT CAAAGTCAAC ATCCTTAGGT 
TCATCAACAC CTGTGTTTTC GATGAGTTCT CCGATATCCC GAAGATTTAA TAATCTTTTT 
GGTAAAACAG GAACATATAA CGTTTCCACC CCAGAGACAA CCAGCTCATC CGTGGAAAAT 
TCCTCTAGTG CTTACTCATT GCTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006244594.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244656.1 Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa32039
Clone ID Related Accession (Same CDS sequence)	XM_006244594.3
Accession Version	XM_006244594.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	adhesion G protein-coupled receptor F5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244594.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGGACCC CAGAAACAGA CCTAGAGTCT 
AGCTGCAGCA CTTATACACT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG TTCTTCTGGA 
ACAACAGTCA TCTACACATG TGAGTTCGTC AGCGTCTACG GAGCCAAAGG CAGTAAGAAC 
ATAGCCGTGA CCTTCACCTC TGTAGGAGAT AATTCCAGCT ACAAAGGGGA AGTGCCCGAG 
GCCCCAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGACTAGAA 
ACAAGTAAAT CCCAGGAGCA GCAAGCCAAC CTAACAATAA CCCCGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACTTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CTTCCGCTGG TATTAAAATC CATCCAAGGT TTTATACCAT GAGGAGGTAT 
CGGGATGGAG CAGAGTCGGT GCTGACGGTG AAGACCTCCA CCAGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATAG CCACCAAAGA TGTCACTGTT 
CACCCTCTGC CTCTGGAGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGC 
ACCAGTTCCC ATCAGTTCAA GTGCTGCATA GAAGAGAACG ATGGGGAAGA GTACATTGTG 
ACTTTCCACG TGGATTCCTC ATCCTTTCCT GCTGAAAGAG AAGTTATTGG CAAGCAGGCA 
TGCTACACAT ACAGTCTCCC AGGAAAGTTA CCATCACGGT GTCCCAAAGA CATTGACGTG 
TTCTGCCACT TTACCAATGC AGCCAACAGC TCAGTCCGGA GCCCATCTAT GAAGCTTACC 
CTTGTTCCAG GGAAGAATAT CACATGCCAG GATCCCATCA TAGGTATTGG GGAACCTGGG 
AAAGTCATCC AGAAGCTGTG CCAGTTTGCA GGTGTTTCTA GAAGCCCTGG ACAGACCATT 
GGTGGAACCG TCACTTATAA ATGTGTAGGC TCCCAGTGGA AGGAGGAGAC AAGGGCCTGC 
ATATCAGCCC CAATCAATGG TTTGCTCCAG TTGGCCAAGG CTTTGATCAA GAGCCCCTCC 
CAGGATCAGA AGCTCCCTAA ATACCTGCGG GACCTTTCTG TTAGCACTGG GAAGGAAGAA 
CAGGATATAC GCTCGTCGCC CGGGAGCCTG GGAGCCATCA TCAGCATCCT TGATTTGCTT 
TCAACAGTTC CCACTCAAGT GAATTCAGAA ATGATGAGGG ATATACTTGC TACCATTAAT 
GTCATCTTAG ACAAGTCCAC CTTGAACTCC TGGGAGAAGT TACTACAGCA ACAGAGCAAC 
CAGAGCTCTC AGTTCCTGCA GTCAGTGGAG AGGTTTTCCA AAGCGCTCGA GCTGGGAGAC 
AGCACCCCTC CCTTCCTCTT CCACCCTAAT GTTCAGATGA AGAGCATGGT GATAAAACGT 
GGCCACGCCC AAATGTACCA ACAGAAATTT GTCTTCACAG ACTCTGACCT ATGGGGTGAT 
GTAGCCATTG ATGAGTGTCA GCTGGGAAGC TTGCAGCCTG ATTCATCGAT CGTTACTGTG 
GCTTTCCCAA CTCTCAAAGC CATCCTGGCC CAGGATGGAC AGAGAAAGAC ACCCTCCAAC 
AGCCTAGTAA TGACCACCAC TGTCAGCCAT AATATCGTCA AGCCTTTTAG GATTTCTATG 
ACATTCAAGA ACAACCATCG TTCGGGTGGC AAGCCACAGT GTGTCTTCTG GAACTTCAGC 
CTTGCCAATA ATACAGGGGG CTGGGATAGC AGTGGGTGCA CTGTGGAAGA TGATGGTAGG 
GACAATAGGG ACAGAGTCTT CTGCAAGTGT AACCACCTGA CGTCATTCTC CATTCTCATG 
TCCCCAGACT CCCCAGACCC AGGATCTCTT TTAAAAATAC TTCTGGATAT CATTTCTTAC 
ATCGGGTTGG GCTTTTCCAT AGTCAGCTTA GCTGCCTGTC TAGTTGTGGA AGCCATGGTG 
TGGAAATCAG TGACCAAGAA CCGAACTTCC TATATGCGCC ACATCTGCAT CGTCAACATT 
GCCCTTTGCC TTCTGATTGC TGACATCTGG TTCATTGTGG CTGGTGCTAT CCATGATGGG 
CATTACCCAC TCAACGAAAC AGCCTGTGTG GCCGCCACAT TCTTCATTCA CTTCTTCTAC 
CTCAGTGTCT TCTTCTGGAT GCTAACTCTG GGCCTCATGC TCTTCTACCG GCTGATTTTC 
ATTCTACATG ACGCGAGCAA GTCCACGCAG AAAGCCATTG CCTTTTCTCT AGGCTATGGC 
TGTCCACTCA TTATCTCATC CATCACAGTG GGGGTTACAC AGCCACAGGA AGTTTACATG 
AGGAAGAATG CATGTTGGCT CAACTGGGAG GACACCAGAG CACTGCTGGC TTTTGCCATC 
CCAGCGTTGA TTATTGTGGT GGTGAACGTG AGCATCACAG TTGTGGTCAT CACCAAGATC 
CTAAGGCCCT CCGTCGGAGA CAAGCCAGGC AAGCAGGAGA AGAGCAGCCT ATTCCAGATC 
AGCAAGAGCA TTGGAGTCCT CACGCCACTC TTGGGGCTCA CTTGGGGTTT TGGTCTGGCC 
ACAGTGATCC AGGGGAGCAA TGCTGTGTTC CACATCATAT TTACTCTCCT CAATGCCTTT 
CAGGGGCTCT TCATTTTGCT CTTTGGCTGC CTCTGGGATC AGAAGGTACA GGAAGCTTTG 
CTGCATAAGT TTTCATTGTC AAGGTGGTCT TCTCAACACT CAAAGTCAAC ATCCTTAGGT 
TCATCAACAC CTGTGTTTTC GATGAGTTCT CCGATATCCC GAAGATTTAA TAATCTTTTT 
GGTAAAACAG GAACATATAA CGTTTCCACC CCAGAGACAA CCAGCTCATC CGTGGAAAAT 
TCCTCTAGTG CTTACTCATT GCTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244656.1
CDS	386..4555
Translation	MKSSRTVTLYFVLIVICSSEATWSRPAEPIVHPLILQEHELAGEELLRPKRAVAVGGPVAEEYTVDVEISFENVSFLESIRAHLNSLRFPVQGNGTDILSMAMTTVCTPTGNDLLCFCEKGYQWPEERCLSSLTCQEHDSALPGRYCNCLKGLPPQGPFCQLPETYITLKIKVRLNIGFQEDLENTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVDYIVEVASAPLPGSIHKANEQVIQNLNQTYKMDYNSFQGTPSNETKFTVTPEFIFEGDNVTLECESEFVSSNTSWFYGEKRSDIQNSDKFSIHTSIINNISLVTRLTIFNFTQHDAGLYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNNPISLNCCSENIANWSRIEWKQEGKINIEGTPETDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSVYGAKGSKNIAVTFTSVGDNSSYKGEVPEAPRHVMEVSTVQSTTGNRLETSKSQEQQANLTITPDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYRDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLESDIMMDPLEASGLCTSSHQFKCCIEENDGEEYIVTFHVDSSSFPAEREVIGKQACYTYSLPGKLPSRCPKDIDVFCHFTNAANSSVRSPSMKLTLVPGKNITCQDPIIGIGEPGKVIQKLCQFAGVSRSPGQTIGGTVTYKCVGSQWKEETRACISAPINGLLQLAKALIKSPSQDQKLPKYLRDLSVSTGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSTLNSWEKLLQQQSNQSSQFLQSVERFSKALELGDSTPPFLFHPNVQMKSMVIKRGHAQMYQQKFVFTDSDLWGDVAIDECQLGSLQPDSSIVTVAFPTLKAILAQDGQRKTPSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCTVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIALCLLIADIWFIVAGAIHDGHYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSVGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSVENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006244594.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244594.3

ORF Insert Sequence:

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGGACCC CAGAAACAGA CCTAGAGTCT 
AGCTGCAGCA CTTATACACT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG TTCTTCTGGA 
ACAACAGTCA TCTACACATG TGAGTTCGTC AGCGTCTACG GAGCCAAAGG CAGTAAGAAC 
ATAGCCGTGA CCTTCACCTC TGTAGGAGAT AATTCCAGCT ACAAAGGGGA AGTGCCCGAG 
GCCCCAAGGC ATGTGATGGA AGTGAGTACA GTTCAGAGCA CCACCGGGAA CAGACTAGAA 
ACAAGTAAAT CCCAGGAGCA GCAAGCCAAC CTAACAATAA CCCCGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACTTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CTTCCGCTGG TATTAAAATC CATCCAAGGT TTTATACCAT GAGGAGGTAT 
CGGGATGGAG CAGAGTCGGT GCTGACGGTG AAGACCTCCA CCAGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATAG CCACCAAAGA TGTCACTGTT 
CACCCTCTGC CTCTGGAGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGC 
ACCAGTTCCC ATCAGTTCAA GTGCTGCATA GAAGAGAACG ATGGGGAAGA GTACATTGTG 
ACTTTCCACG TGGATTCCTC ATCCTTTCCT GCTGAAAGAG AAGTTATTGG CAAGCAGGCA 
TGCTACACAT ACAGTCTCCC AGGAAAGTTA CCATCACGGT GTCCCAAAGA CATTGACGTG 
TTCTGCCACT TTACCAATGC AGCCAACAGC TCAGTCCGGA GCCCATCTAT GAAGCTTACC 
CTTGTTCCAG GGAAGAATAT CACATGCCAG GATCCCATCA TAGGTATTGG GGAACCTGGG 
AAAGTCATCC AGAAGCTGTG CCAGTTTGCA GGTGTTTCTA GAAGCCCTGG ACAGACCATT 
GGTGGAACCG TCACTTATAA ATGTGTAGGC TCCCAGTGGA AGGAGGAGAC AAGGGCCTGC 
ATATCAGCCC CAATCAATGG TTTGCTCCAG TTGGCCAAGG CTTTGATCAA GAGCCCCTCC 
CAGGATCAGA AGCTCCCTAA ATACCTGCGG GACCTTTCTG TTAGCACTGG GAAGGAAGAA 
CAGGATATAC GCTCGTCGCC CGGGAGCCTG GGAGCCATCA TCAGCATCCT TGATTTGCTT 
TCAACAGTTC CCACTCAAGT GAATTCAGAA ATGATGAGGG ATATACTTGC TACCATTAAT 
GTCATCTTAG ACAAGTCCAC CTTGAACTCC TGGGAGAAGT TACTACAGCA ACAGAGCAAC 
CAGAGCTCTC AGTTCCTGCA GTCAGTGGAG AGGTTTTCCA AAGCGCTCGA GCTGGGAGAC 
AGCACCCCTC CCTTCCTCTT CCACCCTAAT GTTCAGATGA AGAGCATGGT GATAAAACGT 
GGCCACGCCC AAATGTACCA ACAGAAATTT GTCTTCACAG ACTCTGACCT ATGGGGTGAT 
GTAGCCATTG ATGAGTGTCA GCTGGGAAGC TTGCAGCCTG ATTCATCGAT CGTTACTGTG 
GCTTTCCCAA CTCTCAAAGC CATCCTGGCC CAGGATGGAC AGAGAAAGAC ACCCTCCAAC 
AGCCTAGTAA TGACCACCAC TGTCAGCCAT AATATCGTCA AGCCTTTTAG GATTTCTATG 
ACATTCAAGA ACAACCATCG TTCGGGTGGC AAGCCACAGT GTGTCTTCTG GAACTTCAGC 
CTTGCCAATA ATACAGGGGG CTGGGATAGC AGTGGGTGCA CTGTGGAAGA TGATGGTAGG 
GACAATAGGG ACAGAGTCTT CTGCAAGTGT AACCACCTGA CGTCATTCTC CATTCTCATG 
TCCCCAGACT CCCCAGACCC AGGATCTCTT TTAAAAATAC TTCTGGATAT CATTTCTTAC 
ATCGGGTTGG GCTTTTCCAT AGTCAGCTTA GCTGCCTGTC TAGTTGTGGA AGCCATGGTG 
TGGAAATCAG TGACCAAGAA CCGAACTTCC TATATGCGCC ACATCTGCAT CGTCAACATT 
GCCCTTTGCC TTCTGATTGC TGACATCTGG TTCATTGTGG CTGGTGCTAT CCATGATGGG 
CATTACCCAC TCAACGAAAC AGCCTGTGTG GCCGCCACAT TCTTCATTCA CTTCTTCTAC 
CTCAGTGTCT TCTTCTGGAT GCTAACTCTG GGCCTCATGC TCTTCTACCG GCTGATTTTC 
ATTCTACATG ACGCGAGCAA GTCCACGCAG AAAGCCATTG CCTTTTCTCT AGGCTATGGC 
TGTCCACTCA TTATCTCATC CATCACAGTG GGGGTTACAC AGCCACAGGA AGTTTACATG 
AGGAAGAATG CATGTTGGCT CAACTGGGAG GACACCAGAG CACTGCTGGC TTTTGCCATC 
CCAGCGTTGA TTATTGTGGT GGTGAACGTG AGCATCACAG TTGTGGTCAT CACCAAGATC 
CTAAGGCCCT CCGTCGGAGA CAAGCCAGGC AAGCAGGAGA AGAGCAGCCT ATTCCAGATC 
AGCAAGAGCA TTGGAGTCCT CACGCCACTC TTGGGGCTCA CTTGGGGTTT TGGTCTGGCC 
ACAGTGATCC AGGGGAGCAA TGCTGTGTTC CACATCATAT TTACTCTCCT CAATGCCTTT 
CAGGGGCTCT TCATTTTGCT CTTTGGCTGC CTCTGGGATC AGAAGGTACA GGAAGCTTTG 
CTGCATAAGT TTTCATTGTC AAGGTGGTCT TCTCAACACT CAAAGTCAAC ATCCTTAGGT 
TCATCAACAC CTGTGTTTTC GATGAGTTCT CCGATATCCC GAAGATTTAA TAATCTTTTT 
GGTAAAACAG GAACATATAA CGTTTCCACC CCAGAGACAA CCAGCTCATC CGTGGAAAAT 
TCCTCTAGTG CTTACTCATT GCTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_006244597.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244659.1 Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.

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CloneID	ORa32041
Clone ID Related Accession (Same CDS sequence)	XM_006244597.3
Accession Version	XM_006244597.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	adhesion G protein-coupled receptor F5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGGACCC CAGAAACAGA CCTAGAGTCT 
AGCTGCAGCA CTTATACACT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG TTCTTCTGGA 
ACAACAGTCA TCTACACATG TGAGTTCGTC AGCGTCTACG GAGCCAAAGG CAGTAAGAAC 
ATAGCCGTGA CCTTCACCTC TGTAGCCAAC CTAACAATAA CCCCGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACTTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CTTCCGCTGG TATTAAAATC CATCCAAGGT TTTATACCAT GAGGAGGTAT 
CGGGATGGAG CAGAGTCGGT GCTGACGGTG AAGACCTCCA CCAGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATAG CCACCAAAGA TGTCACTGTT 
CACCCTCTGC CTCTGGAGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGC 
ACCAGTTCCC ATCAGTTCAA GTGCTGCATA GAAGAGAACG ATGGGGAAGA GTACATTGTG 
ACTTTCCACG TGGATTCCTC ATCCTTTCCT GCTGAAAGAG AAGTTATTGG CAAGCAGGCA 
TGCTACACAT ACAGTCTCCC AGGAAAGTTA CCATCACGGT GTCCCAAAGA CATTGACGTG 
TTCTGCCACT TTACCAATGC AGCCAACAGC TCAGTCCGGA GCCCATCTAT GAAGCTTACC 
CTTGTTCCAG GGAAGAATAT CACATGCCAG GATCCCATCA TAGGTATTGG GGAACCTGGG 
AAAGTCATCC AGAAGCTGTG CCAGTTTGCA GGTGTTTCTA GAAGCCCTGG ACAGACCATT 
GGTGGAACCG TCACTTATAA ATGTGTAGGC TCCCAGTGGA AGGAGGAGAC AAGGGCCTGC 
ATATCAGCCC CAATCAATGG TTTGCTCCAG TTGGCCAAGG CTTTGATCAA GAGCCCCTCC 
CAGGATCAGA AGCTCCCTAA ATACCTGCGG GACCTTTCTG TTAGCACTGG GAAGGAAGAA 
CAGGATATAC GCTCGTCGCC CGGGAGCCTG GGAGCCATCA TCAGCATCCT TGATTTGCTT 
TCAACAGTTC CCACTCAAGT GAATTCAGAA ATGATGAGGG ATATACTTGC TACCATTAAT 
GTCATCTTAG ACAAGTCCAC CTTGAACTCC TGGGAGAAGT TACTACAGCA ACAGAGCAAC 
CAGAGCTCTC AGTTCCTGCA GTCAGTGGAG AGGTTTTCCA AAGCGCTCGA GCTGGGAGAC 
AGCACCCCTC CCTTCCTCTT CCACCCTAAT GTTCAGATGA AGAGCATGGT GATAAAACGT 
GGCCACGCCC AAATGTACCA ACAGAAATTT GTCTTCACAG ACTCTGACCT ATGGGGTGAT 
GTAGCCATTG ATGAGTGTCA GCTGGGAAGC TTGCAGCCTG ATTCATCGAT CGTTACTGTG 
GCTTTCCCAA CTCTCAAAGC CATCCTGGCC CAGGATGGAC AGAGAAAGAC ACCCTCCAAC 
AGCCTAGTAA TGACCACCAC TGTCAGCCAT AATATCGTCA AGCCTTTTAG GATTTCTATG 
ACATTCAAGA ACAACCATCG TTCGGGTGGC AAGCCACAGT GTGTCTTCTG GAACTTCAGC 
CTTGCCAATA ATACAGGGGG CTGGGATAGC AGTGGGTGCA CTGTGGAAGA TGATGGTAGG 
GACAATAGGG ACAGAGTCTT CTGCAAGTGT AACCACCTGA CGTCATTCTC CATTCTCATG 
TCCCCAGACT CCCCAGACCC AGGATCTCTT TTAAAAATAC TTCTGGATAT CATTTCTTAC 
ATCGGGTTGG GCTTTTCCAT AGTCAGCTTA GCTGCCTGTC TAGTTGTGGA AGCCATGGTG 
TGGAAATCAG TGACCAAGAA CCGAACTTCC TATATGCGCC ACATCTGCAT CGTCAACATT 
GCCCTTTGCC TTCTGATTGC TGACATCTGG TTCATTGTGG CTGGTGCTAT CCATGATGGG 
CATTACCCAC TCAACGAAAC AGCCTGTGTG GCCGCCACAT TCTTCATTCA CTTCTTCTAC 
CTCAGTGTCT TCTTCTGGAT GCTAACTCTG GGCCTCATGC TCTTCTACCG GCTGATTTTC 
ATTCTACATG ACGCGAGCAA GTCCACGCAG AAAGCCATTG CCTTTTCTCT AGGCTATGGC 
TGTCCACTCA TTATCTCATC CATCACAGTG GGGGTTACAC AGCCACAGGA AGTTTACATG 
AGGAAGAATG CATGTTGGCT CAACTGGGAG GACACCAGAG CACTGCTGGC TTTTGCCATC 
CCAGCGTTGA TTATTGTGGT GGTGAACGTG AGCATCACAG TTGTGGTCAT CACCAAGATC 
CTAAGGCCCT CCGTCGGAGA CAAGCCAGGC AAGCAGGAGA AGAGCAGCCT ATTCCAGATC 
AGCAAGAGCA TTGGAGTCCT CACGCCACTC TTGGGGCTCA CTTGGGGTTT TGGTCTGGCC 
ACAGTGATCC AGGGGAGCAA TGCTGTGTTC CACATCATAT TTACTCTCCT CAATGCCTTT 
CAGGGGCTCT TCATTTTGCT CTTTGGCTGC CTCTGGGATC AGAAGGTACA GGAAGCTTTG 
CTGCATAAGT TTTCATTGTC AAGGTGGTCT TCTCAACACT CAAAGTCAAC ATCCTTAGGT 
TCATCAACAC CTGTGTTTTC GATGAGTTCT CCGATATCCC GAAGATTTAA TAATCTTTTT 
GGTAAAACAG GAACATATAA CGTTTCCACC CCAGAGACAA CCAGCTCATC CGTGGAAAAT 
TCCTCTAGTG CTTACTCATT GCTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244659.1
CDS	346..4395
Translation	MKSSRTVTLYFVLIVICSSEATWSRPAEPIVHPLILQEHELAGEELLRPKRAVAVGGPVAEEYTVDVEISFENVSFLESIRAHLNSLRFPVQGNGTDILSMAMTTVCTPTGNDLLCFCEKGYQWPEERCLSSLTCQEHDSALPGRYCNCLKGLPPQGPFCQLPETYITLKIKVRLNIGFQEDLENTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVDYIVEVASAPLPGSIHKANEQVIQNLNQTYKMDYNSFQGTPSNETKFTVTPEFIFEGDNVTLECESEFVSSNTSWFYGEKRSDIQNSDKFSIHTSIINNISLVTRLTIFNFTQHDAGLYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNNPISLNCCSENIANWSRIEWKQEGKINIEGTPETDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSVYGAKGSKNIAVTFTSVANLTITPDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYRDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLESDIMMDPLEASGLCTSSHQFKCCIEENDGEEYIVTFHVDSSSFPAEREVIGKQACYTYSLPGKLPSRCPKDIDVFCHFTNAANSSVRSPSMKLTLVPGKNITCQDPIIGIGEPGKVIQKLCQFAGVSRSPGQTIGGTVTYKCVGSQWKEETRACISAPINGLLQLAKALIKSPSQDQKLPKYLRDLSVSTGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSTLNSWEKLLQQQSNQSSQFLQSVERFSKALELGDSTPPFLFHPNVQMKSMVIKRGHAQMYQQKFVFTDSDLWGDVAIDECQLGSLQPDSSIVTVAFPTLKAILAQDGQRKTPSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCTVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIALCLLIADIWFIVAGAIHDGHYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSVGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSVENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_006244597.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244597.3

ORF Insert Sequence:

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGGACCC CAGAAACAGA CCTAGAGTCT 
AGCTGCAGCA CTTATACACT CAAGGCAGAT GGAACCCAGT GTCCCAGTGG TTCTTCTGGA 
ACAACAGTCA TCTACACATG TGAGTTCGTC AGCGTCTACG GAGCCAAAGG CAGTAAGAAC 
ATAGCCGTGA CCTTCACCTC TGTAGCCAAC CTAACAATAA CCCCGGACCC AATTTCTGTT 
TCTGAGGGAC AAAGCTTTTC TATAACTTGC CTCAGCGATG TGAGTAGCTT TGATGAGGTG 
TACTGGAACA CTTCCGCTGG TATTAAAATC CATCCAAGGT TTTATACCAT GAGGAGGTAT 
CGGGATGGAG CAGAGTCGGT GCTGACGGTG AAGACCTCCA CCAGAGAGTG GAACGGAACC 
TACCACTGCA TATTTAGATA TAAGAATTCA TACAGCATAG CCACCAAAGA TGTCACTGTT 
CACCCTCTGC CTCTGGAGTC AGACATCATG ATGGATCCTT TGGAAGCCAG TGGTCTGTGC 
ACCAGTTCCC ATCAGTTCAA GTGCTGCATA GAAGAGAACG ATGGGGAAGA GTACATTGTG 
ACTTTCCACG TGGATTCCTC ATCCTTTCCT GCTGAAAGAG AAGTTATTGG CAAGCAGGCA 
TGCTACACAT ACAGTCTCCC AGGAAAGTTA CCATCACGGT GTCCCAAAGA CATTGACGTG 
TTCTGCCACT TTACCAATGC AGCCAACAGC TCAGTCCGGA GCCCATCTAT GAAGCTTACC 
CTTGTTCCAG GGAAGAATAT CACATGCCAG GATCCCATCA TAGGTATTGG GGAACCTGGG 
AAAGTCATCC AGAAGCTGTG CCAGTTTGCA GGTGTTTCTA GAAGCCCTGG ACAGACCATT 
GGTGGAACCG TCACTTATAA ATGTGTAGGC TCCCAGTGGA AGGAGGAGAC AAGGGCCTGC 
ATATCAGCCC CAATCAATGG TTTGCTCCAG TTGGCCAAGG CTTTGATCAA GAGCCCCTCC 
CAGGATCAGA AGCTCCCTAA ATACCTGCGG GACCTTTCTG TTAGCACTGG GAAGGAAGAA 
CAGGATATAC GCTCGTCGCC CGGGAGCCTG GGAGCCATCA TCAGCATCCT TGATTTGCTT 
TCAACAGTTC CCACTCAAGT GAATTCAGAA ATGATGAGGG ATATACTTGC TACCATTAAT 
GTCATCTTAG ACAAGTCCAC CTTGAACTCC TGGGAGAAGT TACTACAGCA ACAGAGCAAC 
CAGAGCTCTC AGTTCCTGCA GTCAGTGGAG AGGTTTTCCA AAGCGCTCGA GCTGGGAGAC 
AGCACCCCTC CCTTCCTCTT CCACCCTAAT GTTCAGATGA AGAGCATGGT GATAAAACGT 
GGCCACGCCC AAATGTACCA ACAGAAATTT GTCTTCACAG ACTCTGACCT ATGGGGTGAT 
GTAGCCATTG ATGAGTGTCA GCTGGGAAGC TTGCAGCCTG ATTCATCGAT CGTTACTGTG 
GCTTTCCCAA CTCTCAAAGC CATCCTGGCC CAGGATGGAC AGAGAAAGAC ACCCTCCAAC 
AGCCTAGTAA TGACCACCAC TGTCAGCCAT AATATCGTCA AGCCTTTTAG GATTTCTATG 
ACATTCAAGA ACAACCATCG TTCGGGTGGC AAGCCACAGT GTGTCTTCTG GAACTTCAGC 
CTTGCCAATA ATACAGGGGG CTGGGATAGC AGTGGGTGCA CTGTGGAAGA TGATGGTAGG 
GACAATAGGG ACAGAGTCTT CTGCAAGTGT AACCACCTGA CGTCATTCTC CATTCTCATG 
TCCCCAGACT CCCCAGACCC AGGATCTCTT TTAAAAATAC TTCTGGATAT CATTTCTTAC 
ATCGGGTTGG GCTTTTCCAT AGTCAGCTTA GCTGCCTGTC TAGTTGTGGA AGCCATGGTG 
TGGAAATCAG TGACCAAGAA CCGAACTTCC TATATGCGCC ACATCTGCAT CGTCAACATT 
GCCCTTTGCC TTCTGATTGC TGACATCTGG TTCATTGTGG CTGGTGCTAT CCATGATGGG 
CATTACCCAC TCAACGAAAC AGCCTGTGTG GCCGCCACAT TCTTCATTCA CTTCTTCTAC 
CTCAGTGTCT TCTTCTGGAT GCTAACTCTG GGCCTCATGC TCTTCTACCG GCTGATTTTC 
ATTCTACATG ACGCGAGCAA GTCCACGCAG AAAGCCATTG CCTTTTCTCT AGGCTATGGC 
TGTCCACTCA TTATCTCATC CATCACAGTG GGGGTTACAC AGCCACAGGA AGTTTACATG 
AGGAAGAATG CATGTTGGCT CAACTGGGAG GACACCAGAG CACTGCTGGC TTTTGCCATC 
CCAGCGTTGA TTATTGTGGT GGTGAACGTG AGCATCACAG TTGTGGTCAT CACCAAGATC 
CTAAGGCCCT CCGTCGGAGA CAAGCCAGGC AAGCAGGAGA AGAGCAGCCT ATTCCAGATC 
AGCAAGAGCA TTGGAGTCCT CACGCCACTC TTGGGGCTCA CTTGGGGTTT TGGTCTGGCC 
ACAGTGATCC AGGGGAGCAA TGCTGTGTTC CACATCATAT TTACTCTCCT CAATGCCTTT 
CAGGGGCTCT TCATTTTGCT CTTTGGCTGC CTCTGGGATC AGAAGGTACA GGAAGCTTTG 
CTGCATAAGT TTTCATTGTC AAGGTGGTCT TCTCAACACT CAAAGTCAAC ATCCTTAGGT 
TCATCAACAC CTGTGTTTTC GATGAGTTCT CCGATATCCC GAAGATTTAA TAATCTTTTT 
GGTAAAACAG GAACATATAA CGTTTCCACC CCAGAGACAA CCAGCTCATC CGTGGAAAAT 
TCCTCTAGTG CTTACTCATT GCTCAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_008766832.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765054.1 Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.

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CloneID	ORa44402
Clone ID Related Accession (Same CDS sequence)	XM_008766832.2 , XM_008766833.2
Accession Version	XM_008766832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	adhesion G protein-coupled receptor F5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGCCAGA CCTCTAAGGA CGAAGTACAT 
TCAACAGGAA GAGGAAGGGG AGATACAAGT GTGAACACAG GGACCCCAGA AACAGACCTA 
GAGTCTAGCT GCAGCACTTA TACACTCAAG GCAGATGGAA CCCAGTGTCC CAGTGGTTCT 
TCTGGAACAA CAGTCATCTA CACATGTGAG TTCGTCAGCG TCTACGGAGC CAAAGGCAGT 
AAGAACATAG CCGTGACCTT CACCTCTGTA GGAGATAATT CCAGCTACAA AGGGGAAGTG 
CCCGAGGCCC CAAGGCATGT GATGGAAGTG AGTACAGTTC AGAGCACCAC CGGGAACAGA 
CTAGAAACAA GTAAATCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAG CTTTTCTATA ACTTGCCTCA GCGATGTGAG TAGCTTTGAT 
GAGGTGTACT GGAACACTTC CGCTGGTATT AAAATCCATC CAAGGTTTTA TACCATGAGG 
AGGTATCGGG ATGGAGCAGA GTCGGTGCTG ACGGTGAAGA CCTCCACCAG AGAGTGGAAC 
GGAACCTACC ACTGCATATT TAGATATAAG AATTCATACA GCATAGCCAC CAAAGATGTC 
ACTGTTCACC CTCTGCCTCT GGAGTCAGAC ATCATGATGG ATCCTTTGGA AGCCAGTGGT 
CTGTGCACCA GTTCCCATCA GTTCAAGTGC TGCATAGAAG AGAACGATGG GGAAGAGTAC 
ATTGTGACTT TCCACGTGGA TTCCTCATCC TTTCCTGCTG AAAGAGAAGT TATTGGCAAG 
CAGGCATGCT ACACATACAG TCTCCCAGGA AAGTTACCAT CACGGTGTCC CAAAGACATT 
GACGTGTTCT GCCACTTTAC CAATGCAGCC AACAGCTCAG TCCGGAGCCC ATCTATGAAG 
CTTACCCTTG TTCCAGGGAA GAATATCACA TGCCAGGATC CCATCATAGG TATTGGGGAA 
CCTGGGAAAG TCATCCAGAA GCTGTGCCAG TTTGCAGGTG TTTCTAGAAG CCCTGGACAG 
ACCATTGGTG GAACCGTCAC TTATAAATGT GTAGGCTCCC AGTGGAAGGA GGAGACAAGG 
GCCTGCATAT CAGCCCCAAT CAATGGTTTG CTCCAGTTGG CCAAGGCTTT GATCAAGAGC 
CCCTCCCAGG ATCAGAAGCT CCCTAAATAC CTGCGGGACC TTTCTGTTAG CACTGGGAAG 
GAAGAACAGG ATATACGCTC GTCGCCCGGG AGCCTGGGAG CCATCATCAG CATCCTTGAT 
TTGCTTTCAA CAGTTCCCAC TCAAGTGAAT TCAGAAATGA TGAGGGATAT ACTTGCTACC 
ATTAATGTCA TCTTAGACAA GTCCACCTTG AACTCCTGGG AGAAGTTACT ACAGCAACAG 
AGCAACCAGA GCTCTCAGTT CCTGCAGTCA GTGGAGAGGT TTTCCAAAGC GCTCGAGCTG 
GGAGACAGCA CCCCTCCCTT CCTCTTCCAC CCTAATGTTC AGATGAAGAG CATGGTGATA 
AAACGTGGCC ACGCCCAAAT GTACCAACAG AAATTTGTCT TCACAGACTC TGACCTATGG 
GGTGATGTAG CCATTGATGA GTGTCAGCTG GGAAGCTTGC AGCCTGATTC ATCGATCGTT 
ACTGTGGCTT TCCCAACTCT CAAAGCCATC CTGGCCCAGG ATGGACAGAG AAAGACACCC 
TCCAACAGCC TAGTAATGAC CACCACTGTC AGCCATAATA TCGTCAAGCC TTTTAGGATT 
TCTATGACAT TCAAGAACAA CCATCGTTCG GGTGGCAAGC CACAGTGTGT CTTCTGGAAC 
TTCAGCCTTG CCAATAATAC AGGGGGCTGG GATAGCAGTG GGTGCACTGT GGAAGATGAT 
GGTAGGGACA ATAGGGACAG AGTCTTCTGC AAGTGTAACC ACCTGACGTC ATTCTCCATT 
CTCATGTCCC CAGACTCCCC AGACCCAGGA TCTCTTTTAA AAATACTTCT GGATATCATT 
TCTTACATCG GGTTGGGCTT TTCCATAGTC AGCTTAGCTG CCTGTCTAGT TGTGGAAGCC 
ATGGTGTGGA AATCAGTGAC CAAGAACCGA ACTTCCTATA TGCGCCACAT CTGCATCGTC 
AACATTGCCC TTTGCCTTCT GATTGCTGAC ATCTGGTTCA TTGTGGCTGG TGCTATCCAT 
GATGGGCATT ACCCACTCAA CGAAACAGCC TGTGTGGCCG CCACATTCTT CATTCACTTC 
TTCTACCTCA GTGTCTTCTT CTGGATGCTA ACTCTGGGCC TCATGCTCTT CTACCGGCTG 
ATTTTCATTC TACATGACGC GAGCAAGTCC ACGCAGAAAG CCATTGCCTT TTCTCTAGGC 
TATGGCTGTC CACTCATTAT CTCATCCATC ACAGTGGGGG TTACACAGCC ACAGGAAGTT 
TACATGAGGA AGAATGCATG TTGGCTCAAC TGGGAGGACA CCAGAGCACT GCTGGCTTTT 
GCCATCCCAG CGTTGATTAT TGTGGTGGTG AACGTGAGCA TCACAGTTGT GGTCATCACC 
AAGATCCTAA GGCCCTCCGT CGGAGACAAG CCAGGCAAGC AGGAGAAGAG CAGCCTATTC 
CAGATCAGCA AGAGCATTGG AGTCCTCACG CCACTCTTGG GGCTCACTTG GGGTTTTGGT 
CTGGCCACAG TGATCCAGGG GAGCAATGCT GTGTTCCACA TCATATTTAC TCTCCTCAAT 
GCCTTTCAGG GGCTCTTCAT TTTGCTCTTT GGCTGCCTCT GGGATCAGAA GGTACAGGAA 
GCTTTGCTGC ATAAGTTTTC ATTGTCAAGG TGGTCTTCTC AACACTCAAA GTCAACATCC 
TTAGGTTCAT CAACACCTGT GTTTTCGATG AGTTCTCCGA TATCCCGAAG ATTTAATAAT 
CTTTTTGGTA AAACAGGAAC ATATAACGTT TCCACCCCAG AGACAACCAG CTCATCCGTG 
GAAAATTCCT CTAGTGCTTA CTCATTGCTC AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765054.1
CDS	388..4623
Translation	MKSSRTVTLYFVLIVICSSEATWSRPAEPIVHPLILQEHELAGEELLRPKRAVAVGGPVAEEYTVDVEISFENVSFLESIRAHLNSLRFPVQGNGTDILSMAMTTVCTPTGNDLLCFCEKGYQWPEERCLSSLTCQEHDSALPGRYCNCLKGLPPQGPFCQLPETYITLKIKVRLNIGFQEDLENTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVDYIVEVASAPLPGSIHKANEQVIQNLNQTYKMDYNSFQGTPSNETKFTVTPEFIFEGDNVTLECESEFVSSNTSWFYGEKRSDIQNSDKFSIHTSIINNISLVTRLTIFNFTQHDAGLYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNNPISLNCCSENIANWSRIEWKQEGKINIEGQTSKDEVHSTGRGRGDTSVNTGTPETDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSVYGAKGSKNIAVTFTSVGDNSSYKGEVPEAPRHVMEVSTVQSTTGNRLETSKSQEQQANLTITPDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYRDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLESDIMMDPLEASGLCTSSHQFKCCIEENDGEEYIVTFHVDSSSFPAEREVIGKQACYTYSLPGKLPSRCPKDIDVFCHFTNAANSSVRSPSMKLTLVPGKNITCQDPIIGIGEPGKVIQKLCQFAGVSRSPGQTIGGTVTYKCVGSQWKEETRACISAPINGLLQLAKALIKSPSQDQKLPKYLRDLSVSTGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSTLNSWEKLLQQQSNQSSQFLQSVERFSKALELGDSTPPFLFHPNVQMKSMVIKRGHAQMYQQKFVFTDSDLWGDVAIDECQLGSLQPDSSIVTVAFPTLKAILAQDGQRKTPSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCTVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIALCLLIADIWFIVAGAIHDGHYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSVGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSVENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_008766832.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766832.2

ORF Insert Sequence:

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGCCAGA CCTCTAAGGA CGAAGTACAT 
TCAACAGGAA GAGGAAGGGG AGATACAAGT GTGAACACAG GGACCCCAGA AACAGACCTA 
GAGTCTAGCT GCAGCACTTA TACACTCAAG GCAGATGGAA CCCAGTGTCC CAGTGGTTCT 
TCTGGAACAA CAGTCATCTA CACATGTGAG TTCGTCAGCG TCTACGGAGC CAAAGGCAGT 
AAGAACATAG CCGTGACCTT CACCTCTGTA GGAGATAATT CCAGCTACAA AGGGGAAGTG 
CCCGAGGCCC CAAGGCATGT GATGGAAGTG AGTACAGTTC AGAGCACCAC CGGGAACAGA 
CTAGAAACAA GTAAATCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAG CTTTTCTATA ACTTGCCTCA GCGATGTGAG TAGCTTTGAT 
GAGGTGTACT GGAACACTTC CGCTGGTATT AAAATCCATC CAAGGTTTTA TACCATGAGG 
AGGTATCGGG ATGGAGCAGA GTCGGTGCTG ACGGTGAAGA CCTCCACCAG AGAGTGGAAC 
GGAACCTACC ACTGCATATT TAGATATAAG AATTCATACA GCATAGCCAC CAAAGATGTC 
ACTGTTCACC CTCTGCCTCT GGAGTCAGAC ATCATGATGG ATCCTTTGGA AGCCAGTGGT 
CTGTGCACCA GTTCCCATCA GTTCAAGTGC TGCATAGAAG AGAACGATGG GGAAGAGTAC 
ATTGTGACTT TCCACGTGGA TTCCTCATCC TTTCCTGCTG AAAGAGAAGT TATTGGCAAG 
CAGGCATGCT ACACATACAG TCTCCCAGGA AAGTTACCAT CACGGTGTCC CAAAGACATT 
GACGTGTTCT GCCACTTTAC CAATGCAGCC AACAGCTCAG TCCGGAGCCC ATCTATGAAG 
CTTACCCTTG TTCCAGGGAA GAATATCACA TGCCAGGATC CCATCATAGG TATTGGGGAA 
CCTGGGAAAG TCATCCAGAA GCTGTGCCAG TTTGCAGGTG TTTCTAGAAG CCCTGGACAG 
ACCATTGGTG GAACCGTCAC TTATAAATGT GTAGGCTCCC AGTGGAAGGA GGAGACAAGG 
GCCTGCATAT CAGCCCCAAT CAATGGTTTG CTCCAGTTGG CCAAGGCTTT GATCAAGAGC 
CCCTCCCAGG ATCAGAAGCT CCCTAAATAC CTGCGGGACC TTTCTGTTAG CACTGGGAAG 
GAAGAACAGG ATATACGCTC GTCGCCCGGG AGCCTGGGAG CCATCATCAG CATCCTTGAT 
TTGCTTTCAA CAGTTCCCAC TCAAGTGAAT TCAGAAATGA TGAGGGATAT ACTTGCTACC 
ATTAATGTCA TCTTAGACAA GTCCACCTTG AACTCCTGGG AGAAGTTACT ACAGCAACAG 
AGCAACCAGA GCTCTCAGTT CCTGCAGTCA GTGGAGAGGT TTTCCAAAGC GCTCGAGCTG 
GGAGACAGCA CCCCTCCCTT CCTCTTCCAC CCTAATGTTC AGATGAAGAG CATGGTGATA 
AAACGTGGCC ACGCCCAAAT GTACCAACAG AAATTTGTCT TCACAGACTC TGACCTATGG 
GGTGATGTAG CCATTGATGA GTGTCAGCTG GGAAGCTTGC AGCCTGATTC ATCGATCGTT 
ACTGTGGCTT TCCCAACTCT CAAAGCCATC CTGGCCCAGG ATGGACAGAG AAAGACACCC 
TCCAACAGCC TAGTAATGAC CACCACTGTC AGCCATAATA TCGTCAAGCC TTTTAGGATT 
TCTATGACAT TCAAGAACAA CCATCGTTCG GGTGGCAAGC CACAGTGTGT CTTCTGGAAC 
TTCAGCCTTG CCAATAATAC AGGGGGCTGG GATAGCAGTG GGTGCACTGT GGAAGATGAT 
GGTAGGGACA ATAGGGACAG AGTCTTCTGC AAGTGTAACC ACCTGACGTC ATTCTCCATT 
CTCATGTCCC CAGACTCCCC AGACCCAGGA TCTCTTTTAA AAATACTTCT GGATATCATT 
TCTTACATCG GGTTGGGCTT TTCCATAGTC AGCTTAGCTG CCTGTCTAGT TGTGGAAGCC 
ATGGTGTGGA AATCAGTGAC CAAGAACCGA ACTTCCTATA TGCGCCACAT CTGCATCGTC 
AACATTGCCC TTTGCCTTCT GATTGCTGAC ATCTGGTTCA TTGTGGCTGG TGCTATCCAT 
GATGGGCATT ACCCACTCAA CGAAACAGCC TGTGTGGCCG CCACATTCTT CATTCACTTC 
TTCTACCTCA GTGTCTTCTT CTGGATGCTA ACTCTGGGCC TCATGCTCTT CTACCGGCTG 
ATTTTCATTC TACATGACGC GAGCAAGTCC ACGCAGAAAG CCATTGCCTT TTCTCTAGGC 
TATGGCTGTC CACTCATTAT CTCATCCATC ACAGTGGGGG TTACACAGCC ACAGGAAGTT 
TACATGAGGA AGAATGCATG TTGGCTCAAC TGGGAGGACA CCAGAGCACT GCTGGCTTTT 
GCCATCCCAG CGTTGATTAT TGTGGTGGTG AACGTGAGCA TCACAGTTGT GGTCATCACC 
AAGATCCTAA GGCCCTCCGT CGGAGACAAG CCAGGCAAGC AGGAGAAGAG CAGCCTATTC 
CAGATCAGCA AGAGCATTGG AGTCCTCACG CCACTCTTGG GGCTCACTTG GGGTTTTGGT 
CTGGCCACAG TGATCCAGGG GAGCAATGCT GTGTTCCACA TCATATTTAC TCTCCTCAAT 
GCCTTTCAGG GGCTCTTCAT TTTGCTCTTT GGCTGCCTCT GGGATCAGAA GGTACAGGAA 
GCTTTGCTGC ATAAGTTTTC ATTGTCAAGG TGGTCTTCTC AACACTCAAA GTCAACATCC 
TTAGGTTCAT CAACACCTGT GTTTTCGATG AGTTCTCCGA TATCCCGAAG ATTTAATAAT 
CTTTTTGGTA AAACAGGAAC ATATAACGTT TCCACCCCAG AGACAACCAG CTCATCCGTG 
GAAAATTCCT CTAGTGCTTA CTCATTGCTC AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_008766833.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765055.1 Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa44402
Clone ID Related Accession (Same CDS sequence)	XM_008766832.2 , XM_008766833.2
Accession Version	XM_008766833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	adhesion G protein-coupled receptor F5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGCCAGA CCTCTAAGGA CGAAGTACAT 
TCAACAGGAA GAGGAAGGGG AGATACAAGT GTGAACACAG GGACCCCAGA AACAGACCTA 
GAGTCTAGCT GCAGCACTTA TACACTCAAG GCAGATGGAA CCCAGTGTCC CAGTGGTTCT 
TCTGGAACAA CAGTCATCTA CACATGTGAG TTCGTCAGCG TCTACGGAGC CAAAGGCAGT 
AAGAACATAG CCGTGACCTT CACCTCTGTA GGAGATAATT CCAGCTACAA AGGGGAAGTG 
CCCGAGGCCC CAAGGCATGT GATGGAAGTG AGTACAGTTC AGAGCACCAC CGGGAACAGA 
CTAGAAACAA GTAAATCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAG CTTTTCTATA ACTTGCCTCA GCGATGTGAG TAGCTTTGAT 
GAGGTGTACT GGAACACTTC CGCTGGTATT AAAATCCATC CAAGGTTTTA TACCATGAGG 
AGGTATCGGG ATGGAGCAGA GTCGGTGCTG ACGGTGAAGA CCTCCACCAG AGAGTGGAAC 
GGAACCTACC ACTGCATATT TAGATATAAG AATTCATACA GCATAGCCAC CAAAGATGTC 
ACTGTTCACC CTCTGCCTCT GGAGTCAGAC ATCATGATGG ATCCTTTGGA AGCCAGTGGT 
CTGTGCACCA GTTCCCATCA GTTCAAGTGC TGCATAGAAG AGAACGATGG GGAAGAGTAC 
ATTGTGACTT TCCACGTGGA TTCCTCATCC TTTCCTGCTG AAAGAGAAGT TATTGGCAAG 
CAGGCATGCT ACACATACAG TCTCCCAGGA AAGTTACCAT CACGGTGTCC CAAAGACATT 
GACGTGTTCT GCCACTTTAC CAATGCAGCC AACAGCTCAG TCCGGAGCCC ATCTATGAAG 
CTTACCCTTG TTCCAGGGAA GAATATCACA TGCCAGGATC CCATCATAGG TATTGGGGAA 
CCTGGGAAAG TCATCCAGAA GCTGTGCCAG TTTGCAGGTG TTTCTAGAAG CCCTGGACAG 
ACCATTGGTG GAACCGTCAC TTATAAATGT GTAGGCTCCC AGTGGAAGGA GGAGACAAGG 
GCCTGCATAT CAGCCCCAAT CAATGGTTTG CTCCAGTTGG CCAAGGCTTT GATCAAGAGC 
CCCTCCCAGG ATCAGAAGCT CCCTAAATAC CTGCGGGACC TTTCTGTTAG CACTGGGAAG 
GAAGAACAGG ATATACGCTC GTCGCCCGGG AGCCTGGGAG CCATCATCAG CATCCTTGAT 
TTGCTTTCAA CAGTTCCCAC TCAAGTGAAT TCAGAAATGA TGAGGGATAT ACTTGCTACC 
ATTAATGTCA TCTTAGACAA GTCCACCTTG AACTCCTGGG AGAAGTTACT ACAGCAACAG 
AGCAACCAGA GCTCTCAGTT CCTGCAGTCA GTGGAGAGGT TTTCCAAAGC GCTCGAGCTG 
GGAGACAGCA CCCCTCCCTT CCTCTTCCAC CCTAATGTTC AGATGAAGAG CATGGTGATA 
AAACGTGGCC ACGCCCAAAT GTACCAACAG AAATTTGTCT TCACAGACTC TGACCTATGG 
GGTGATGTAG CCATTGATGA GTGTCAGCTG GGAAGCTTGC AGCCTGATTC ATCGATCGTT 
ACTGTGGCTT TCCCAACTCT CAAAGCCATC CTGGCCCAGG ATGGACAGAG AAAGACACCC 
TCCAACAGCC TAGTAATGAC CACCACTGTC AGCCATAATA TCGTCAAGCC TTTTAGGATT 
TCTATGACAT TCAAGAACAA CCATCGTTCG GGTGGCAAGC CACAGTGTGT CTTCTGGAAC 
TTCAGCCTTG CCAATAATAC AGGGGGCTGG GATAGCAGTG GGTGCACTGT GGAAGATGAT 
GGTAGGGACA ATAGGGACAG AGTCTTCTGC AAGTGTAACC ACCTGACGTC ATTCTCCATT 
CTCATGTCCC CAGACTCCCC AGACCCAGGA TCTCTTTTAA AAATACTTCT GGATATCATT 
TCTTACATCG GGTTGGGCTT TTCCATAGTC AGCTTAGCTG CCTGTCTAGT TGTGGAAGCC 
ATGGTGTGGA AATCAGTGAC CAAGAACCGA ACTTCCTATA TGCGCCACAT CTGCATCGTC 
AACATTGCCC TTTGCCTTCT GATTGCTGAC ATCTGGTTCA TTGTGGCTGG TGCTATCCAT 
GATGGGCATT ACCCACTCAA CGAAACAGCC TGTGTGGCCG CCACATTCTT CATTCACTTC 
TTCTACCTCA GTGTCTTCTT CTGGATGCTA ACTCTGGGCC TCATGCTCTT CTACCGGCTG 
ATTTTCATTC TACATGACGC GAGCAAGTCC ACGCAGAAAG CCATTGCCTT TTCTCTAGGC 
TATGGCTGTC CACTCATTAT CTCATCCATC ACAGTGGGGG TTACACAGCC ACAGGAAGTT 
TACATGAGGA AGAATGCATG TTGGCTCAAC TGGGAGGACA CCAGAGCACT GCTGGCTTTT 
GCCATCCCAG CGTTGATTAT TGTGGTGGTG AACGTGAGCA TCACAGTTGT GGTCATCACC 
AAGATCCTAA GGCCCTCCGT CGGAGACAAG CCAGGCAAGC AGGAGAAGAG CAGCCTATTC 
CAGATCAGCA AGAGCATTGG AGTCCTCACG CCACTCTTGG GGCTCACTTG GGGTTTTGGT 
CTGGCCACAG TGATCCAGGG GAGCAATGCT GTGTTCCACA TCATATTTAC TCTCCTCAAT 
GCCTTTCAGG GGCTCTTCAT TTTGCTCTTT GGCTGCCTCT GGGATCAGAA GGTACAGGAA 
GCTTTGCTGC ATAAGTTTTC ATTGTCAAGG TGGTCTTCTC AACACTCAAA GTCAACATCC 
TTAGGTTCAT CAACACCTGT GTTTTCGATG AGTTCTCCGA TATCCCGAAG ATTTAATAAT 
CTTTTTGGTA AAACAGGAAC ATATAACGTT TCCACCCCAG AGACAACCAG CTCATCCGTG 
GAAAATTCCT CTAGTGCTTA CTCATTGCTC AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765055.1
CDS	319..4554
Translation	MKSSRTVTLYFVLIVICSSEATWSRPAEPIVHPLILQEHELAGEELLRPKRAVAVGGPVAEEYTVDVEISFENVSFLESIRAHLNSLRFPVQGNGTDILSMAMTTVCTPTGNDLLCFCEKGYQWPEERCLSSLTCQEHDSALPGRYCNCLKGLPPQGPFCQLPETYITLKIKVRLNIGFQEDLENTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVDYIVEVASAPLPGSIHKANEQVIQNLNQTYKMDYNSFQGTPSNETKFTVTPEFIFEGDNVTLECESEFVSSNTSWFYGEKRSDIQNSDKFSIHTSIINNISLVTRLTIFNFTQHDAGLYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNNPISLNCCSENIANWSRIEWKQEGKINIEGQTSKDEVHSTGRGRGDTSVNTGTPETDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSVYGAKGSKNIAVTFTSVGDNSSYKGEVPEAPRHVMEVSTVQSTTGNRLETSKSQEQQANLTITPDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYRDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLESDIMMDPLEASGLCTSSHQFKCCIEENDGEEYIVTFHVDSSSFPAEREVIGKQACYTYSLPGKLPSRCPKDIDVFCHFTNAANSSVRSPSMKLTLVPGKNITCQDPIIGIGEPGKVIQKLCQFAGVSRSPGQTIGGTVTYKCVGSQWKEETRACISAPINGLLQLAKALIKSPSQDQKLPKYLRDLSVSTGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSTLNSWEKLLQQQSNQSSQFLQSVERFSKALELGDSTPPFLFHPNVQMKSMVIKRGHAQMYQQKFVFTDSDLWGDVAIDECQLGSLQPDSSIVTVAFPTLKAILAQDGQRKTPSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCTVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIALCLLIADIWFIVAGAIHDGHYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSVGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSVENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_008766833.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766833.2

ORF Insert Sequence:

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGCCAGA CCTCTAAGGA CGAAGTACAT 
TCAACAGGAA GAGGAAGGGG AGATACAAGT GTGAACACAG GGACCCCAGA AACAGACCTA 
GAGTCTAGCT GCAGCACTTA TACACTCAAG GCAGATGGAA CCCAGTGTCC CAGTGGTTCT 
TCTGGAACAA CAGTCATCTA CACATGTGAG TTCGTCAGCG TCTACGGAGC CAAAGGCAGT 
AAGAACATAG CCGTGACCTT CACCTCTGTA GGAGATAATT CCAGCTACAA AGGGGAAGTG 
CCCGAGGCCC CAAGGCATGT GATGGAAGTG AGTACAGTTC AGAGCACCAC CGGGAACAGA 
CTAGAAACAA GTAAATCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAG CTTTTCTATA ACTTGCCTCA GCGATGTGAG TAGCTTTGAT 
GAGGTGTACT GGAACACTTC CGCTGGTATT AAAATCCATC CAAGGTTTTA TACCATGAGG 
AGGTATCGGG ATGGAGCAGA GTCGGTGCTG ACGGTGAAGA CCTCCACCAG AGAGTGGAAC 
GGAACCTACC ACTGCATATT TAGATATAAG AATTCATACA GCATAGCCAC CAAAGATGTC 
ACTGTTCACC CTCTGCCTCT GGAGTCAGAC ATCATGATGG ATCCTTTGGA AGCCAGTGGT 
CTGTGCACCA GTTCCCATCA GTTCAAGTGC TGCATAGAAG AGAACGATGG GGAAGAGTAC 
ATTGTGACTT TCCACGTGGA TTCCTCATCC TTTCCTGCTG AAAGAGAAGT TATTGGCAAG 
CAGGCATGCT ACACATACAG TCTCCCAGGA AAGTTACCAT CACGGTGTCC CAAAGACATT 
GACGTGTTCT GCCACTTTAC CAATGCAGCC AACAGCTCAG TCCGGAGCCC ATCTATGAAG 
CTTACCCTTG TTCCAGGGAA GAATATCACA TGCCAGGATC CCATCATAGG TATTGGGGAA 
CCTGGGAAAG TCATCCAGAA GCTGTGCCAG TTTGCAGGTG TTTCTAGAAG CCCTGGACAG 
ACCATTGGTG GAACCGTCAC TTATAAATGT GTAGGCTCCC AGTGGAAGGA GGAGACAAGG 
GCCTGCATAT CAGCCCCAAT CAATGGTTTG CTCCAGTTGG CCAAGGCTTT GATCAAGAGC 
CCCTCCCAGG ATCAGAAGCT CCCTAAATAC CTGCGGGACC TTTCTGTTAG CACTGGGAAG 
GAAGAACAGG ATATACGCTC GTCGCCCGGG AGCCTGGGAG CCATCATCAG CATCCTTGAT 
TTGCTTTCAA CAGTTCCCAC TCAAGTGAAT TCAGAAATGA TGAGGGATAT ACTTGCTACC 
ATTAATGTCA TCTTAGACAA GTCCACCTTG AACTCCTGGG AGAAGTTACT ACAGCAACAG 
AGCAACCAGA GCTCTCAGTT CCTGCAGTCA GTGGAGAGGT TTTCCAAAGC GCTCGAGCTG 
GGAGACAGCA CCCCTCCCTT CCTCTTCCAC CCTAATGTTC AGATGAAGAG CATGGTGATA 
AAACGTGGCC ACGCCCAAAT GTACCAACAG AAATTTGTCT TCACAGACTC TGACCTATGG 
GGTGATGTAG CCATTGATGA GTGTCAGCTG GGAAGCTTGC AGCCTGATTC ATCGATCGTT 
ACTGTGGCTT TCCCAACTCT CAAAGCCATC CTGGCCCAGG ATGGACAGAG AAAGACACCC 
TCCAACAGCC TAGTAATGAC CACCACTGTC AGCCATAATA TCGTCAAGCC TTTTAGGATT 
TCTATGACAT TCAAGAACAA CCATCGTTCG GGTGGCAAGC CACAGTGTGT CTTCTGGAAC 
TTCAGCCTTG CCAATAATAC AGGGGGCTGG GATAGCAGTG GGTGCACTGT GGAAGATGAT 
GGTAGGGACA ATAGGGACAG AGTCTTCTGC AAGTGTAACC ACCTGACGTC ATTCTCCATT 
CTCATGTCCC CAGACTCCCC AGACCCAGGA TCTCTTTTAA AAATACTTCT GGATATCATT 
TCTTACATCG GGTTGGGCTT TTCCATAGTC AGCTTAGCTG CCTGTCTAGT TGTGGAAGCC 
ATGGTGTGGA AATCAGTGAC CAAGAACCGA ACTTCCTATA TGCGCCACAT CTGCATCGTC 
AACATTGCCC TTTGCCTTCT GATTGCTGAC ATCTGGTTCA TTGTGGCTGG TGCTATCCAT 
GATGGGCATT ACCCACTCAA CGAAACAGCC TGTGTGGCCG CCACATTCTT CATTCACTTC 
TTCTACCTCA GTGTCTTCTT CTGGATGCTA ACTCTGGGCC TCATGCTCTT CTACCGGCTG 
ATTTTCATTC TACATGACGC GAGCAAGTCC ACGCAGAAAG CCATTGCCTT TTCTCTAGGC 
TATGGCTGTC CACTCATTAT CTCATCCATC ACAGTGGGGG TTACACAGCC ACAGGAAGTT 
TACATGAGGA AGAATGCATG TTGGCTCAAC TGGGAGGACA CCAGAGCACT GCTGGCTTTT 
GCCATCCCAG CGTTGATTAT TGTGGTGGTG AACGTGAGCA TCACAGTTGT GGTCATCACC 
AAGATCCTAA GGCCCTCCGT CGGAGACAAG CCAGGCAAGC AGGAGAAGAG CAGCCTATTC 
CAGATCAGCA AGAGCATTGG AGTCCTCACG CCACTCTTGG GGCTCACTTG GGGTTTTGGT 
CTGGCCACAG TGATCCAGGG GAGCAATGCT GTGTTCCACA TCATATTTAC TCTCCTCAAT 
GCCTTTCAGG GGCTCTTCAT TTTGCTCTTT GGCTGCCTCT GGGATCAGAA GGTACAGGAA 
GCTTTGCTGC ATAAGTTTTC ATTGTCAAGG TGGTCTTCTC AACACTCAAA GTCAACATCC 
TTAGGTTCAT CAACACCTGT GTTTTCGATG AGTTCTCCGA TATCCCGAAG ATTTAATAAT 
CTTTTTGGTA AAACAGGAAC ATATAACGTT TCCACCCCAG AGACAACCAG CTCATCCGTG 
GAAAATTCCT CTAGTGCTTA CTCATTGCTC AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_008766834.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765056.1 Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa44403
Clone ID Related Accession (Same CDS sequence)	XM_008766834.2
Accession Version	XM_008766834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	adhesion G protein-coupled receptor F5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGCCAGA CCTCTAAGGA CGAAGTACAT 
TCAACAGGAA GAGGAAGGGG AGATACAAGT GTGAACACAG GGACCCCAGA AACAGACCTA 
GAGTCTAGCT GCAGCACTTA TACACTCAAG GCAGATGGAA CCCAGTGTCC CAGTGGTTCT 
TCTGGAACAA CAGTCATCTA CACATGTGAG TTCGTCAGCG TCTACGGAGC CAAAGGCAGT 
AAGAACATAG CCGTGACCTT CACCTCTGTA GGAGATAATT CCAGCTACAA AGGGGAAGTG 
CCCGAGGCCC CAAGGCATGT GATGGAAGTG AGTACAGTTC AGAGCACCAC CGGGAACAGA 
CTAGAAACAA GTAAATCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAG CTTTTCTATA ACTTGCCTCA GCGATGTGAG TAGCTTTGAT 
GAGGTGTACT GGAACACTTC CGCTGGTATT AAAATCCATC CAAGGTTTTA TACCATGAGG 
AGGTATCGGG ATGGAGCAGA GTCGGTGCTG ACGGTGAAGA CCTCCACCAG AGAGTGGAAC 
GGAACCTACC ACTGCATATT TAGATATAAG AATTCATACA GCATAGCCAC CAAAGATGTC 
ACTGTTCACC CTCTGCCTCT GGAGTCAGAC ATCATGATGG ATCCTTTGGA AGCCAGTGGT 
CTGTGCACCA GTTCCCATCA GTTCAAGTGC TGCATAGAAG AGAACGATGG GGAAGAGTAC 
ATTGTGACTT TCCACGTGGA TTCCTCATCC TTTCCTGCTG AAAGAGAAGT TATTGGCAAG 
CAGGCATGCT ACACATACAG TCTCCCAGGA AAGTTACCAT CACGGTGTCC CAAAGACATT 
GACGTGTTCT GCCACTTTAC CAATGCAGCC AACAGCTCAG TCCGGAGCCC ATCTATGAAG 
CTTACCCTTG TTCCAGGGAA GAATATCACA TGCCAGGATC CCATCATAGG TATTGGGGAA 
CCTGGGAAAG TCATCCAGAA GCTGTGCCAG TTTGCAGGTG TTTCTAGAAG CCCTGGACAG 
ACCATTGGTG GAACCGTCAC TTATAAATGT GTAGGCTCCC AGTGGAAGGA GGAGACAAGG 
GCCTGCATAT CAGCCCCAAT CAATGGTTTG CTCCAGTTGG CCAAGGCTTT GATCAAGAGC 
CCCTCCCAGG ATCAGAAGCT CCCTAAATAC CTGCGGGACC TTTCTGTTAG CACTGGGAAG 
GAAGAACAGG ATATACGCTC GTCGCCCGGG AGCCTGGGAG CCATCATCAG CATCCTTGAT 
TTGCTTTCAA CAGTTCCCAC TCAAGTGAAT TCAGAAATGA TGAGGGATAT ACTTGCTACC 
ATTAATGTCA TCTTAGACAA GTCCACCTTG AACTCCTGGG AGAAGTTACT ACAGCAACAG 
AGCAACCAGA GCTCTCAGTT CCTGCAGTCA GTGGAGAGGT TTTCCAAAGC GCTCGAGCTG 
GGAGACAGCA CCCCTCCCTT CCTCTTCCAC CCTAATGTTC AGATGAAGAG CATGGTGATA 
AAACGTGGCC ACGCCCAAAT GTACCAACAG AAATTTGTCT TCACAGACTC TGACCTATGG 
GGTGATGTAG CCATTGATGA GTGTCAGCTG GGAAGCTTGC AGCCTGATTC ATCGATCGTT 
ACTGTGGCTT TCCCAACTCT CAAAGCCATC CTGGCCCAGG ATGGACAGAG AAAGACACCC 
TCCAACAGCC TAGTAATGAC CACCACTGTC AGCCATAATA TCGTCAAGCC TTTTAGGATT 
TCTATGACAT TCAAGAACAA CCATCGTTCG GGTGGCAAGC CACAGTGTGT CTTCTGGAAC 
TTCAGCCTTG CCAATAATAC AGGGGGCTGG GATAGCAGTG GGTGCACTGT GGAAGATGAT 
GGTAGGGACA ATAGGGACAG AGTCTTCTGC AAGTGTAACC ACCTGACGTC ATTCTCCATT 
CTCATGTCCC CAGACTCCCC AGACCCAGGA TCTCTTTTAA AAATACTTCT GGATATCATT 
TCTTACATCG GGTTGGGCTT TTCCATAGTC AGCTTAGCTG CCTGTCTAGT TGTGGAAGCC 
ATGGTGTGGA AATCAGTGAC CAAGAACCGA ACTTCCTATA TGCGCCACAT CTGCATCGTC 
AACATTGCCC TTTGCCTTCT GATTGCTGAC ATCTGGTTCA TTGTGGCTGG TGCTATCCAT 
GATGGGCATT ACCCACTCAA CGAAACAGCC TGTGTGGCCG CCACATTCTT CATTCACTTC 
TTCTACCTCA GTGTCTTCTT CTGGATGCTA ACTCTGGGCC TCATGCTCTT CTACCGGCTG 
ATTTTCATTC TACATGACGC GAGCAAGTCC ACGCAGAAAG CCATTGCCTT TTCTCTAGGC 
TATGGCTGTC CACTCATTAT CTCATCCATC ACAGTGGGGG TTACACAGCC ACAGGAAGTT 
TACATGAGGA AGAATGCATG TTGGCTCAAC TGGGAGGACA CCAGAGCACT GCTGGCTTTT 
GCCATCCCAG CGTTGATTAT TGTGGTGGTG AACGTGAGCA TCACAGTTGT GGTCATCACC 
AAGATCCTAA GGCCCTCCGT CGGAGACAAG CCAGGCAAGC AGGAGAAGAG CAGCCTATTC 
CAGATCAGCA AGAGCATTGG AGTCCTCACG CCACTCTTGG GGCTCACTTG GGGTTTTGGT 
CTGGCCACAG TGATCCAGGG GAGCAATGCT GTGTTCCACA TCATATTTAC TCTCCTCAAT 
GCCTTTCAGG GGCTCTTCAT TTTGCTCTTT GGCTGCCTCT GGGATCAGAA GGTACAGGAA 
GCTTTGCTGC ATAAGTTTTC ATTGTCAAGG TGGTCTTCTC AACACTCAAA GTCAACATCC 
TTAGGTTCAT CAACACCTGT GTTTTCGATG AGTTCTCCGA TATCCCGAAG ATTTAATAAT 
CTTTTTGGTA AAACAGCAAC CCCAAAGGTC CCCCAAGGCA ATGATGGACA CCTTGTTATC 
AGTGGCAGTT CATCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765056.1
CDS	390..4610
Translation	MKSSRTVTLYFVLIVICSSEATWSRPAEPIVHPLILQEHELAGEELLRPKRAVAVGGPVAEEYTVDVEISFENVSFLESIRAHLNSLRFPVQGNGTDILSMAMTTVCTPTGNDLLCFCEKGYQWPEERCLSSLTCQEHDSALPGRYCNCLKGLPPQGPFCQLPETYITLKIKVRLNIGFQEDLENTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVDYIVEVASAPLPGSIHKANEQVIQNLNQTYKMDYNSFQGTPSNETKFTVTPEFIFEGDNVTLECESEFVSSNTSWFYGEKRSDIQNSDKFSIHTSIINNISLVTRLTIFNFTQHDAGLYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNNPISLNCCSENIANWSRIEWKQEGKINIEGQTSKDEVHSTGRGRGDTSVNTGTPETDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSVYGAKGSKNIAVTFTSVGDNSSYKGEVPEAPRHVMEVSTVQSTTGNRLETSKSQEQQANLTITPDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYRDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLESDIMMDPLEASGLCTSSHQFKCCIEENDGEEYIVTFHVDSSSFPAEREVIGKQACYTYSLPGKLPSRCPKDIDVFCHFTNAANSSVRSPSMKLTLVPGKNITCQDPIIGIGEPGKVIQKLCQFAGVSRSPGQTIGGTVTYKCVGSQWKEETRACISAPINGLLQLAKALIKSPSQDQKLPKYLRDLSVSTGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSTLNSWEKLLQQQSNQSSQFLQSVERFSKALELGDSTPPFLFHPNVQMKSMVIKRGHAQMYQQKFVFTDSDLWGDVAIDECQLGSLQPDSSIVTVAFPTLKAILAQDGQRKTPSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCTVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIALCLLIADIWFIVAGAIHDGHYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSVGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTATPKVPQGNDGHLVISGSSSS

Target ORF information:

RefSeq Version	XM_008766834.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766834.2

ORF Insert Sequence:

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGCCAGA CCTCTAAGGA CGAAGTACAT 
TCAACAGGAA GAGGAAGGGG AGATACAAGT GTGAACACAG GGACCCCAGA AACAGACCTA 
GAGTCTAGCT GCAGCACTTA TACACTCAAG GCAGATGGAA CCCAGTGTCC CAGTGGTTCT 
TCTGGAACAA CAGTCATCTA CACATGTGAG TTCGTCAGCG TCTACGGAGC CAAAGGCAGT 
AAGAACATAG CCGTGACCTT CACCTCTGTA GGAGATAATT CCAGCTACAA AGGGGAAGTG 
CCCGAGGCCC CAAGGCATGT GATGGAAGTG AGTACAGTTC AGAGCACCAC CGGGAACAGA 
CTAGAAACAA GTAAATCCCA GGAGCAGCAA GCCAACCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAG CTTTTCTATA ACTTGCCTCA GCGATGTGAG TAGCTTTGAT 
GAGGTGTACT GGAACACTTC CGCTGGTATT AAAATCCATC CAAGGTTTTA TACCATGAGG 
AGGTATCGGG ATGGAGCAGA GTCGGTGCTG ACGGTGAAGA CCTCCACCAG AGAGTGGAAC 
GGAACCTACC ACTGCATATT TAGATATAAG AATTCATACA GCATAGCCAC CAAAGATGTC 
ACTGTTCACC CTCTGCCTCT GGAGTCAGAC ATCATGATGG ATCCTTTGGA AGCCAGTGGT 
CTGTGCACCA GTTCCCATCA GTTCAAGTGC TGCATAGAAG AGAACGATGG GGAAGAGTAC 
ATTGTGACTT TCCACGTGGA TTCCTCATCC TTTCCTGCTG AAAGAGAAGT TATTGGCAAG 
CAGGCATGCT ACACATACAG TCTCCCAGGA AAGTTACCAT CACGGTGTCC CAAAGACATT 
GACGTGTTCT GCCACTTTAC CAATGCAGCC AACAGCTCAG TCCGGAGCCC ATCTATGAAG 
CTTACCCTTG TTCCAGGGAA GAATATCACA TGCCAGGATC CCATCATAGG TATTGGGGAA 
CCTGGGAAAG TCATCCAGAA GCTGTGCCAG TTTGCAGGTG TTTCTAGAAG CCCTGGACAG 
ACCATTGGTG GAACCGTCAC TTATAAATGT GTAGGCTCCC AGTGGAAGGA GGAGACAAGG 
GCCTGCATAT CAGCCCCAAT CAATGGTTTG CTCCAGTTGG CCAAGGCTTT GATCAAGAGC 
CCCTCCCAGG ATCAGAAGCT CCCTAAATAC CTGCGGGACC TTTCTGTTAG CACTGGGAAG 
GAAGAACAGG ATATACGCTC GTCGCCCGGG AGCCTGGGAG CCATCATCAG CATCCTTGAT 
TTGCTTTCAA CAGTTCCCAC TCAAGTGAAT TCAGAAATGA TGAGGGATAT ACTTGCTACC 
ATTAATGTCA TCTTAGACAA GTCCACCTTG AACTCCTGGG AGAAGTTACT ACAGCAACAG 
AGCAACCAGA GCTCTCAGTT CCTGCAGTCA GTGGAGAGGT TTTCCAAAGC GCTCGAGCTG 
GGAGACAGCA CCCCTCCCTT CCTCTTCCAC CCTAATGTTC AGATGAAGAG CATGGTGATA 
AAACGTGGCC ACGCCCAAAT GTACCAACAG AAATTTGTCT TCACAGACTC TGACCTATGG 
GGTGATGTAG CCATTGATGA GTGTCAGCTG GGAAGCTTGC AGCCTGATTC ATCGATCGTT 
ACTGTGGCTT TCCCAACTCT CAAAGCCATC CTGGCCCAGG ATGGACAGAG AAAGACACCC 
TCCAACAGCC TAGTAATGAC CACCACTGTC AGCCATAATA TCGTCAAGCC TTTTAGGATT 
TCTATGACAT TCAAGAACAA CCATCGTTCG GGTGGCAAGC CACAGTGTGT CTTCTGGAAC 
TTCAGCCTTG CCAATAATAC AGGGGGCTGG GATAGCAGTG GGTGCACTGT GGAAGATGAT 
GGTAGGGACA ATAGGGACAG AGTCTTCTGC AAGTGTAACC ACCTGACGTC ATTCTCCATT 
CTCATGTCCC CAGACTCCCC AGACCCAGGA TCTCTTTTAA AAATACTTCT GGATATCATT 
TCTTACATCG GGTTGGGCTT TTCCATAGTC AGCTTAGCTG CCTGTCTAGT TGTGGAAGCC 
ATGGTGTGGA AATCAGTGAC CAAGAACCGA ACTTCCTATA TGCGCCACAT CTGCATCGTC 
AACATTGCCC TTTGCCTTCT GATTGCTGAC ATCTGGTTCA TTGTGGCTGG TGCTATCCAT 
GATGGGCATT ACCCACTCAA CGAAACAGCC TGTGTGGCCG CCACATTCTT CATTCACTTC 
TTCTACCTCA GTGTCTTCTT CTGGATGCTA ACTCTGGGCC TCATGCTCTT CTACCGGCTG 
ATTTTCATTC TACATGACGC GAGCAAGTCC ACGCAGAAAG CCATTGCCTT TTCTCTAGGC 
TATGGCTGTC CACTCATTAT CTCATCCATC ACAGTGGGGG TTACACAGCC ACAGGAAGTT 
TACATGAGGA AGAATGCATG TTGGCTCAAC TGGGAGGACA CCAGAGCACT GCTGGCTTTT 
GCCATCCCAG CGTTGATTAT TGTGGTGGTG AACGTGAGCA TCACAGTTGT GGTCATCACC 
AAGATCCTAA GGCCCTCCGT CGGAGACAAG CCAGGCAAGC AGGAGAAGAG CAGCCTATTC 
CAGATCAGCA AGAGCATTGG AGTCCTCACG CCACTCTTGG GGCTCACTTG GGGTTTTGGT 
CTGGCCACAG TGATCCAGGG GAGCAATGCT GTGTTCCACA TCATATTTAC TCTCCTCAAT 
GCCTTTCAGG GGCTCTTCAT TTTGCTCTTT GGCTGCCTCT GGGATCAGAA GGTACAGGAA 
GCTTTGCTGC ATAAGTTTTC ATTGTCAAGG TGGTCTTCTC AACACTCAAA GTCAACATCC 
TTAGGTTCAT CAACACCTGT GTTTTCGATG AGTTCTCCGA TATCCCGAAG ATTTAATAAT 
CTTTTTGGTA AAACAGCAAC CCCAAAGGTC CCCCAAGGCA ATGATGGACA CCTTGTTATC 
AGTGGCAGTT CATCCTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrf5 ( XM_008766835.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765057.1 Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.

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CloneID	ORa44404
Clone ID Related Accession (Same CDS sequence)	XM_008766835.2
Accession Version	XM_008766835.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	adhesion G protein-coupled receptor F5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGCCAGA CCTCTAAGGA CGAAGTACAT 
TCAACAGGAA GAGGAAGGGG AGATACAAGT GTGAACACAG GGACCCCAGA AACAGACCTA 
GAGTCTAGCT GCAGCACTTA TACACTCAAG GCAGATGGAA CCCAGTGTCC CAGTGGTTCT 
TCTGGAACAA CAGTCATCTA CACATGTGAG TTCGTCAGCG TCTACGGAGC CAAAGGCAGT 
AAGAACATAG CCGTGACCTT CACCTCTGTA GCCAACCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAG CTTTTCTATA ACTTGCCTCA GCGATGTGAG TAGCTTTGAT 
GAGGTGTACT GGAACACTTC CGCTGGTATT AAAATCCATC CAAGGTTTTA TACCATGAGG 
AGGTATCGGG ATGGAGCAGA GTCGGTGCTG ACGGTGAAGA CCTCCACCAG AGAGTGGAAC 
GGAACCTACC ACTGCATATT TAGATATAAG AATTCATACA GCATAGCCAC CAAAGATGTC 
ACTGTTCACC CTCTGCCTCT GGAGTCAGAC ATCATGATGG ATCCTTTGGA AGCCAGTGGT 
CTGTGCACCA GTTCCCATCA GTTCAAGTGC TGCATAGAAG AGAACGATGG GGAAGAGTAC 
ATTGTGACTT TCCACGTGGA TTCCTCATCC TTTCCTGCTG AAAGAGAAGT TATTGGCAAG 
CAGGCATGCT ACACATACAG TCTCCCAGGA AAGTTACCAT CACGGTGTCC CAAAGACATT 
GACGTGTTCT GCCACTTTAC CAATGCAGCC AACAGCTCAG TCCGGAGCCC ATCTATGAAG 
CTTACCCTTG TTCCAGGGAA GAATATCACA TGCCAGGATC CCATCATAGG TATTGGGGAA 
CCTGGGAAAG TCATCCAGAA GCTGTGCCAG TTTGCAGGTG TTTCTAGAAG CCCTGGACAG 
ACCATTGGTG GAACCGTCAC TTATAAATGT GTAGGCTCCC AGTGGAAGGA GGAGACAAGG 
GCCTGCATAT CAGCCCCAAT CAATGGTTTG CTCCAGTTGG CCAAGGCTTT GATCAAGAGC 
CCCTCCCAGG ATCAGAAGCT CCCTAAATAC CTGCGGGACC TTTCTGTTAG CACTGGGAAG 
GAAGAACAGG ATATACGCTC GTCGCCCGGG AGCCTGGGAG CCATCATCAG CATCCTTGAT 
TTGCTTTCAA CAGTTCCCAC TCAAGTGAAT TCAGAAATGA TGAGGGATAT ACTTGCTACC 
ATTAATGTCA TCTTAGACAA GTCCACCTTG AACTCCTGGG AGAAGTTACT ACAGCAACAG 
AGCAACCAGA GCTCTCAGTT CCTGCAGTCA GTGGAGAGGT TTTCCAAAGC GCTCGAGCTG 
GGAGACAGCA CCCCTCCCTT CCTCTTCCAC CCTAATGTTC AGATGAAGAG CATGGTGATA 
AAACGTGGCC ACGCCCAAAT GTACCAACAG AAATTTGTCT TCACAGACTC TGACCTATGG 
GGTGATGTAG CCATTGATGA GTGTCAGCTG GGAAGCTTGC AGCCTGATTC ATCGATCGTT 
ACTGTGGCTT TCCCAACTCT CAAAGCCATC CTGGCCCAGG ATGGACAGAG AAAGACACCC 
TCCAACAGCC TAGTAATGAC CACCACTGTC AGCCATAATA TCGTCAAGCC TTTTAGGATT 
TCTATGACAT TCAAGAACAA CCATCGTTCG GGTGGCAAGC CACAGTGTGT CTTCTGGAAC 
TTCAGCCTTG CCAATAATAC AGGGGGCTGG GATAGCAGTG GGTGCACTGT GGAAGATGAT 
GGTAGGGACA ATAGGGACAG AGTCTTCTGC AAGTGTAACC ACCTGACGTC ATTCTCCATT 
CTCATGTCCC CAGACTCCCC AGACCCAGGA TCTCTTTTAA AAATACTTCT GGATATCATT 
TCTTACATCG GGTTGGGCTT TTCCATAGTC AGCTTAGCTG CCTGTCTAGT TGTGGAAGCC 
ATGGTGTGGA AATCAGTGAC CAAGAACCGA ACTTCCTATA TGCGCCACAT CTGCATCGTC 
AACATTGCCC TTTGCCTTCT GATTGCTGAC ATCTGGTTCA TTGTGGCTGG TGCTATCCAT 
GATGGGCATT ACCCACTCAA CGAAACAGCC TGTGTGGCCG CCACATTCTT CATTCACTTC 
TTCTACCTCA GTGTCTTCTT CTGGATGCTA ACTCTGGGCC TCATGCTCTT CTACCGGCTG 
ATTTTCATTC TACATGACGC GAGCAAGTCC ACGCAGAAAG CCATTGCCTT TTCTCTAGGC 
TATGGCTGTC CACTCATTAT CTCATCCATC ACAGTGGGGG TTACACAGCC ACAGGAAGTT 
TACATGAGGA AGAATGCATG TTGGCTCAAC TGGGAGGACA CCAGAGCACT GCTGGCTTTT 
GCCATCCCAG CGTTGATTAT TGTGGTGGTG AACGTGAGCA TCACAGTTGT GGTCATCACC 
AAGATCCTAA GGCCCTCCGT CGGAGACAAG CCAGGCAAGC AGGAGAAGAG CAGCCTATTC 
CAGATCAGCA AGAGCATTGG AGTCCTCACG CCACTCTTGG GGCTCACTTG GGGTTTTGGT 
CTGGCCACAG TGATCCAGGG GAGCAATGCT GTGTTCCACA TCATATTTAC TCTCCTCAAT 
GCCTTTCAGG GGCTCTTCAT TTTGCTCTTT GGCTGCCTCT GGGATCAGAA GGTACAGGAA 
GCTTTGCTGC ATAAGTTTTC ATTGTCAAGG TGGTCTTCTC AACACTCAAA GTCAACATCC 
TTAGGTTCAT CAACACCTGT GTTTTCGATG AGTTCTCCGA TATCCCGAAG ATTTAATAAT 
CTTTTTGGTA AAACAGGAAC ATATAACGTT TCCACCCCAG AGACAACCAG CTCATCCGTG 
GAAAATTCCT CTAGTGCTTA CTCATTGCTC AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765057.1
CDS	388..4503
Translation	MKSSRTVTLYFVLIVICSSEATWSRPAEPIVHPLILQEHELAGEELLRPKRAVAVGGPVAEEYTVDVEISFENVSFLESIRAHLNSLRFPVQGNGTDILSMAMTTVCTPTGNDLLCFCEKGYQWPEERCLSSLTCQEHDSALPGRYCNCLKGLPPQGPFCQLPETYITLKIKVRLNIGFQEDLENTSSALYRSYKTDLERAFRAGYRTLPGFRSVTVTQFTKGSVVVDYIVEVASAPLPGSIHKANEQVIQNLNQTYKMDYNSFQGTPSNETKFTVTPEFIFEGDNVTLECESEFVSSNTSWFYGEKRSDIQNSDKFSIHTSIINNISLVTRLTIFNFTQHDAGLYGCNVTLDIFEYGTVRKLDVTPIRILAKEERKVVCDNNPISLNCCSENIANWSRIEWKQEGKINIEGQTSKDEVHSTGRGRGDTSVNTGTPETDLESSCSTYTLKADGTQCPSGSSGTTVIYTCEFVSVYGAKGSKNIAVTFTSVANLTITPDPISVSEGQSFSITCLSDVSSFDEVYWNTSAGIKIHPRFYTMRRYRDGAESVLTVKTSTREWNGTYHCIFRYKNSYSIATKDVTVHPLPLESDIMMDPLEASGLCTSSHQFKCCIEENDGEEYIVTFHVDSSSFPAEREVIGKQACYTYSLPGKLPSRCPKDIDVFCHFTNAANSSVRSPSMKLTLVPGKNITCQDPIIGIGEPGKVIQKLCQFAGVSRSPGQTIGGTVTYKCVGSQWKEETRACISAPINGLLQLAKALIKSPSQDQKLPKYLRDLSVSTGKEEQDIRSSPGSLGAIISILDLLSTVPTQVNSEMMRDILATINVILDKSTLNSWEKLLQQQSNQSSQFLQSVERFSKALELGDSTPPFLFHPNVQMKSMVIKRGHAQMYQQKFVFTDSDLWGDVAIDECQLGSLQPDSSIVTVAFPTLKAILAQDGQRKTPSNSLVMTTTVSHNIVKPFRISMTFKNNHRSGGKPQCVFWNFSLANNTGGWDSSGCTVEDDGRDNRDRVFCKCNHLTSFSILMSPDSPDPGSLLKILLDIISYIGLGFSIVSLAACLVVEAMVWKSVTKNRTSYMRHICIVNIALCLLIADIWFIVAGAIHDGHYPLNETACVAATFFIHFFYLSVFFWMLTLGLMLFYRLIFILHDASKSTQKAIAFSLGYGCPLIISSITVGVTQPQEVYMRKNACWLNWEDTRALLAFAIPALIIVVVNVSITVVVITKILRPSVGDKPGKQEKSSLFQISKSIGVLTPLLGLTWGFGLATVIQGSNAVFHIIFTLLNAFQGLFILLFGCLWDQKVQEALLHKFSLSRWSSQHSKSTSLGSSTPVFSMSSPISRRFNNLFGKTGTYNVSTPETTSSSVENSSSAYSLLN

Target ORF information:

RefSeq Version	XM_008766835.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adhesion G protein-coupled receptor F5 (Adgrf5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766835.2

ORF Insert Sequence:

ATGAAATCGT CAAGGACTGT CACGCTCTAC TTTGTGCTTA TTGTGATTTG TTCCTCAGAA 
GCTACATGGA GCAGGCCAGC AGAGCCCATT GTCCATCCTT TGATTCTCCA AGAACATGAA 
CTAGCAGGGG AAGAGCTACT GAGGCCAAAA AGAGCAGTTG CAGTGGGTGG TCCTGTGGCA 
GAAGAATACA CCGTGGATGT TGAGATCAGT TTTGAAAATG TCTCCTTCCT GGAGTCCATC 
AGAGCTCACT TAAACAGCCT CCGTTTTCCA GTTCAGGGGA ACGGCACTGA CATTTTGAGT 
ATGGCAATGA CTACAGTCTG CACTCCTACT GGGAATGACC TCTTGTGCTT CTGCGAGAAA 
GGCTACCAGT GGCCTGAGGA AAGGTGTCTC TCCTCTCTCA CTTGTCAAGA GCATGACAGC 
GCCCTGCCAG GCCGATACTG CAATTGTCTG AAAGGACTTC CTCCCCAAGG ACCTTTCTGC 
CAGCTCCCAG AAACATACAT TACCTTAAAA ATCAAAGTCA GACTGAACAT AGGATTTCAA 
GAAGACCTAG AGAACACATC CTCTGCCCTC TATAGGTCCT ACAAGACTGA TCTGGAAAGA 
GCGTTCCGAG CGGGTTACAG AACTTTACCA GGATTCAGAT CGGTGACTGT GACACAGTTC 
ACCAAGGGCA GCGTGGTTGT GGACTACATA GTCGAGGTTG CATCGGCACC ACTGCCTGGA 
TCAATTCATA AAGCCAATGA GCAAGTCATA CAGAACCTCA ACCAGACTTA CAAAATGGAC 
TACAACTCCT TCCAAGGAAC ACCAAGCAAT GAAACAAAGT TCACTGTGAC ACCAGAGTTC 
ATCTTTGAAG GAGACAATGT AACTCTGGAA TGCGAAAGTG AATTCGTGTC CTCCAACACA 
TCTTGGTTCT ATGGAGAAAA ACGGTCTGAC ATCCAGAACA GTGACAAATT CTCCATTCAC 
ACCTCAATCA TCAACAATAT AAGCCTGGTC ACCCGCCTCA CTATTTTCAA CTTCACACAG 
CATGATGCAG GCTTATATGG TTGCAATGTG ACACTGGATA TTTTTGAATA TGGGACAGTC 
AGAAAATTAG ATGTCACTCC CATCCGGATT TTGGCCAAGG AAGAGAGAAA GGTGGTGTGT 
GACAATAACC CTATATCGTT GAACTGCTGC AGCGAGAATA TCGCGAACTG GAGCAGAATC 
GAGTGGAAAC AGGAGGGGAA AATAAACATT GAAGGCCAGA CCTCTAAGGA CGAAGTACAT 
TCAACAGGAA GAGGAAGGGG AGATACAAGT GTGAACACAG GGACCCCAGA AACAGACCTA 
GAGTCTAGCT GCAGCACTTA TACACTCAAG GCAGATGGAA CCCAGTGTCC CAGTGGTTCT 
TCTGGAACAA CAGTCATCTA CACATGTGAG TTCGTCAGCG TCTACGGAGC CAAAGGCAGT 
AAGAACATAG CCGTGACCTT CACCTCTGTA GCCAACCTAA CAATAACCCC GGACCCAATT 
TCTGTTTCTG AGGGACAAAG CTTTTCTATA ACTTGCCTCA GCGATGTGAG TAGCTTTGAT 
GAGGTGTACT GGAACACTTC CGCTGGTATT AAAATCCATC CAAGGTTTTA TACCATGAGG 
AGGTATCGGG ATGGAGCAGA GTCGGTGCTG ACGGTGAAGA CCTCCACCAG AGAGTGGAAC 
GGAACCTACC ACTGCATATT TAGATATAAG AATTCATACA GCATAGCCAC CAAAGATGTC 
ACTGTTCACC CTCTGCCTCT GGAGTCAGAC ATCATGATGG ATCCTTTGGA AGCCAGTGGT 
CTGTGCACCA GTTCCCATCA GTTCAAGTGC TGCATAGAAG AGAACGATGG GGAAGAGTAC 
ATTGTGACTT TCCACGTGGA TTCCTCATCC TTTCCTGCTG AAAGAGAAGT TATTGGCAAG 
CAGGCATGCT ACACATACAG TCTCCCAGGA AAGTTACCAT CACGGTGTCC CAAAGACATT 
GACGTGTTCT GCCACTTTAC CAATGCAGCC AACAGCTCAG TCCGGAGCCC ATCTATGAAG 
CTTACCCTTG TTCCAGGGAA GAATATCACA TGCCAGGATC CCATCATAGG TATTGGGGAA 
CCTGGGAAAG TCATCCAGAA GCTGTGCCAG TTTGCAGGTG TTTCTAGAAG CCCTGGACAG 
ACCATTGGTG GAACCGTCAC TTATAAATGT GTAGGCTCCC AGTGGAAGGA GGAGACAAGG 
GCCTGCATAT CAGCCCCAAT CAATGGTTTG CTCCAGTTGG CCAAGGCTTT GATCAAGAGC 
CCCTCCCAGG ATCAGAAGCT CCCTAAATAC CTGCGGGACC TTTCTGTTAG CACTGGGAAG 
GAAGAACAGG ATATACGCTC GTCGCCCGGG AGCCTGGGAG CCATCATCAG CATCCTTGAT 
TTGCTTTCAA CAGTTCCCAC TCAAGTGAAT TCAGAAATGA TGAGGGATAT ACTTGCTACC 
ATTAATGTCA TCTTAGACAA GTCCACCTTG AACTCCTGGG AGAAGTTACT ACAGCAACAG 
AGCAACCAGA GCTCTCAGTT CCTGCAGTCA GTGGAGAGGT TTTCCAAAGC GCTCGAGCTG 
GGAGACAGCA CCCCTCCCTT CCTCTTCCAC CCTAATGTTC AGATGAAGAG CATGGTGATA 
AAACGTGGCC ACGCCCAAAT GTACCAACAG AAATTTGTCT TCACAGACTC TGACCTATGG 
GGTGATGTAG CCATTGATGA GTGTCAGCTG GGAAGCTTGC AGCCTGATTC ATCGATCGTT 
ACTGTGGCTT TCCCAACTCT CAAAGCCATC CTGGCCCAGG ATGGACAGAG AAAGACACCC 
TCCAACAGCC TAGTAATGAC CACCACTGTC AGCCATAATA TCGTCAAGCC TTTTAGGATT 
TCTATGACAT TCAAGAACAA CCATCGTTCG GGTGGCAAGC CACAGTGTGT CTTCTGGAAC 
TTCAGCCTTG CCAATAATAC AGGGGGCTGG GATAGCAGTG GGTGCACTGT GGAAGATGAT 
GGTAGGGACA ATAGGGACAG AGTCTTCTGC AAGTGTAACC ACCTGACGTC ATTCTCCATT 
CTCATGTCCC CAGACTCCCC AGACCCAGGA TCTCTTTTAA AAATACTTCT GGATATCATT 
TCTTACATCG GGTTGGGCTT TTCCATAGTC AGCTTAGCTG CCTGTCTAGT TGTGGAAGCC 
ATGGTGTGGA AATCAGTGAC CAAGAACCGA ACTTCCTATA TGCGCCACAT CTGCATCGTC 
AACATTGCCC TTTGCCTTCT GATTGCTGAC ATCTGGTTCA TTGTGGCTGG TGCTATCCAT 
GATGGGCATT ACCCACTCAA CGAAACAGCC TGTGTGGCCG CCACATTCTT CATTCACTTC 
TTCTACCTCA GTGTCTTCTT CTGGATGCTA ACTCTGGGCC TCATGCTCTT CTACCGGCTG 
ATTTTCATTC TACATGACGC GAGCAAGTCC ACGCAGAAAG CCATTGCCTT TTCTCTAGGC 
TATGGCTGTC CACTCATTAT CTCATCCATC ACAGTGGGGG TTACACAGCC ACAGGAAGTT 
TACATGAGGA AGAATGCATG TTGGCTCAAC TGGGAGGACA CCAGAGCACT GCTGGCTTTT 
GCCATCCCAG CGTTGATTAT TGTGGTGGTG AACGTGAGCA TCACAGTTGT GGTCATCACC 
AAGATCCTAA GGCCCTCCGT CGGAGACAAG CCAGGCAAGC AGGAGAAGAG CAGCCTATTC 
CAGATCAGCA AGAGCATTGG AGTCCTCACG CCACTCTTGG GGCTCACTTG GGGTTTTGGT 
CTGGCCACAG TGATCCAGGG GAGCAATGCT GTGTTCCACA TCATATTTAC TCTCCTCAAT 
GCCTTTCAGG GGCTCTTCAT TTTGCTCTTT GGCTGCCTCT GGGATCAGAA GGTACAGGAA 
GCTTTGCTGC ATAAGTTTTC ATTGTCAAGG TGGTCTTCTC AACACTCAAA GTCAACATCC 
TTAGGTTCAT CAACACCTGT GTTTTCGATG AGTTCTCCGA TATCCCGAAG ATTTAATAAT 
CTTTTTGGTA AAACAGGAAC ATATAACGTT TCCACCCCAG AGACAACCAG CTCATCCGTG 
GAAAATTCCT CTAGTGCTTA CTCATTGCTC AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Developmental vascular remodeling defects and postnatal kidney failure in mice lacking Gpr116 (Adgrf5) and Eltd1 (Adgrl4).
PloS one12(8)e0183166(2017)
Lu S,Liu S,Wietelmann A,Kojonazarov B,Atzberger A,Tang C,Schermuly RT,Gr?ne HJ,Offermanns S

Targeted Disruption of Ig-Hepta/Gpr116 Causes Emphysema-like Symptoms That Are Associated with Alveolar Macrophage Activation.
The Journal of biological chemistry290(17)11032-40(2015 Apr)
Ariestanti DM,Ando H,Hirose S,Nakamura N

Orphan G protein-coupled receptor GPR116 regulates pulmonary surfactant pool size.
American journal of respiratory cell and molecular biology49(3)348-57(2013 Sep)
Bridges JP,Ludwig MG,Mueller M,Kinzel B,Sato A,Xu Y,Whitsett JA,Ikegami M

Essential regulation of lung surfactant homeostasis by the orphan G protein-coupled receptor GPR116.
Cell reports3(5)1457-64(2013 May)
Yang MY,Hilton MB,Seaman S,Haines DC,Nagashima K,Burks CM,Tessarollo L,Ivanova PT,Brown HA,Umstead TM,Floros J,Chroneos ZC,St Croix B

Adipose tissue deletion of Gpr116 impairs insulin sensitivity through modulation of adipose function.
FEBS letters586(20)3618-25(2012 Oct)
Nie T,Hui X,Gao X,Li K,Lin W,Xiang X,Ding M,Kuang Y,Xu A,Fei J,Wang Z,Wu D

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Adgrf5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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