Wee1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wee1
Entrez Gene ID	22390
Full Name	WEE 1 homolog 1 (S. pombe)
Synonyms	Wee1A
General protein information	Preferred Names WEE 1 homolog 1 (S. pombe) Names wee1-like protein kinase WEE1 tyrosine kinase wee1A kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_009516.3	NP_033542.2	wee1-like protein kinase isoform 1
XM_006507585.3	XP_006507648.1	wee1-like protein kinase isoform X1
NM_001355058.1	NP_001341987.1	wee1-like protein kinase isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wee1	NP_033542.2
Rattus norvegicus (Norway rat)	Wee1	NP_001012760.1
Danio rerio (zebrafish)	wee1	NP_001005770.1
Homo sapiens (human)	WEE1	NP_003381.1
	WEE1	XP_002808123.1
Drosophila melanogaster (fruit fly)	wee	NP_001260167.1
Pan troglodytes (chimpanzee)	WEE1	XP_521839.2
Canis lupus familiaris (dog)	WEE1	XP_534051.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007798	XP_317709.4
Gallus gallus (chicken)	WEE1	NP_001026352.2

Gene Ontology
Bibliography

Related articles in PubMed

Lacking of palladin leads to multiple cellular events changes which contribute to NTD.
Tan J, Chen XJ, Shen CL, Zhang HX, Tang LY, Lu SY, Wu WT, Kuang Y, Fei J, Wang ZG
Neural development12(1)4(2017 Mar)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

MicroRNA-219-5p Inhibits Morphine-Induced Apoptosis by Targeting Key Cell Cycle Regulator WEE1.
Lou W, Zhang X, Hu XY, Hu AR
Medical science monitor : international medical journal of experimental and clinical research221872-9(2016 Jun)

WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis.
Vassilopoulos A, Tominaga Y, Kim HS, Lahusen T, Li B, Yu H, Gius D, Deng CX
Oncogene34(23)3023-35(2015 Jun)

Involvement of Wee1 in the circadian rhythm-dependent intestinal damage induced by docetaxel.
Obi-Ioka Y, Ushijima K, Kusama M, Ishikawa-Kobayashi E, Fujimura A
The Journal of pharmacology and experimental therapeutics347(1)242-8(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Morphine significantly downregulated the expression of miRNA-219-5p, which targets WEE1 to suppress Tyr15 expressions and activate Cdc2, thus inhibiting the morphine-induced macrophage apoptosis.
Title: MicroRNA-219-5p Inhibits Morphine-Induced Apoptosis by Targeting Key Cell Cycle Regulator WEE1.

Results revealed that WEE1 is indispensable for maintaining genomic stability and it functions as a haploinsufficient tumor suppressor.
Title: WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis.

Wee1 is directly or indirectly involved in the mechanism of the circadian rhythm-dependent changes in docetaxel-induced intestinal damage.
Title: Involvement of Wee1 in the circadian rhythm-dependent intestinal damage induced by docetaxel.

In this study, we found that the chromosomal wee1 gene is also down-regulated by KLF3.
Title: Mouse Wee1 gene is repressed by Kr?ppel-like factor 3 (KLF3) via interaction with multiple upstream elements.

The induction of Wee1 under hypoxia was confirmed both at the mRNA and protein levels.
Title: Hypoxia induces Wee1 expression and attenuates hydrogen peroxide-induced endothelial damage in MS1 cells.

The following Wee1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wee1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20694	NM_009516.3 Latest version!	Mus musculus WEE 1 homolog 1 (S. pombe) (Wee1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu39454	NM_001355058.1 Latest version!	Mus musculus WEE 1 homolog 1 (S. pombe) (Wee1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu39454	XM_006507585.3 Latest version!	Mus musculus WEE 1 homolog 1 (S. pombe) (Wee1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wee1 ( NM_009516.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033542.2 Mus musculus WEE 1 homolog 1 (S. pombe) (Wee1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20694
Clone ID Related Accession (Same CDS sequence)	NM_009516.3
Accession Version	NM_009516.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	wee1-like protein kinase isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ130595.1, AK011212.1, AK039572.1 and BY631896.1. On Sep 15, 2006 this sequence version replaced NM_009516.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK011212.1, SRR1660813.71489.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCCACCCGCC GCGTAGGAGC CGCTTACAGT 
CTGCGGCAGA AGCTCATCTT CTCGCCGGGC AGCGACTGTG AGGAAGAGGA GGAGGAGGAG 
GAGGAAGGCA GCGGCCACAG TACGGGGGAG GACTCGGCCT TCCAGGAACC GGATTCGCCC 
CTGCCTTCTG CACGCAGCCC TGCCGAGGCC GAGGCCGAGC GCCGCCGTCG CTCGCCCGGC 
GCGGAGCCCA GCAGCCCGGG AGAGCTGGAG GACGACTTGC TGCTGCAGGG CGGCGGCGGA 
GGCGCGCAAG CGGCGGGTGG AGGCGCCGAG GGCGACTCTT GGGAGGAGGA GGGTTTCGGC 
TCCTCGTCGC CCGTCAAATC ACCTTCGACC GCCTACTTTC TGAGCAGCCC GTTCTCGCCA 
GTGCGCTGCG GCGGCCCAGG GGATGCCTCC CCGCAGGGTT GCGGGGCTCC GCGAGCCATG 
GACGACCCCT GCTCGCCGCA GCCCGACTAC CCGAGCACCC CTCCGCACAA GACCTTCCGC 
AAGCTGCGCC TGTTCGACAC GCCGCACACT CCCAAGAGTT TGCTTTCCAA AGCGCGAGTT 
ATTGATTCCG GCTCTGTTAA ACTCCGGGGT AGTTCTCTAT TCATGGACAC AGAAAAGTCA 
GGAAAAAGAG AATTCGATAC ACGGCAAACT CCCCAAGTGA ATATTAACCC CTTTACTCCG 
GATCCTGTAC TGCTCCATTC CTCAGGACGG TGTCGTGGGA GAAAGAGAGC GTATTTTAAT 
GATTCCTCTG AAGACATGGA AGCCAGTGAT TATGAGTTTG AAGATGAAAC AAGACCTGCC 
AAAAGAATTA CAATTACTGA AAGCAACATG AAGTCACGGT ATACAACTGA ATTTCATGAG 
CTGGAGAAAA TTGGTTCTGG AGAATTTGGT TCTGTGTTTA AATGTGTGAA GAGGTTAGAT 
GGATGCATTT ATGCCATTAA ACGATCAAAA AAACCATTGG CTGGCTCTGT TGATGAGCAG 
AATGCTTTGA GAGAAGTGTA TGCTCACGCT GTGCTTGGAC AGCACCCCCA CGTCGTTCGC 
TATTTCTCTG CCTGGGCAGA GGATGACCAC ATGCTTATAC AGAACGAATA CTGTAATGGT 
GGGAGTTTAG CTGATGCTAT AAGTGAGAAC TACAGAGTCA TGAGCTACTT GACTGAAGTA 
GAGCTGAAGG ATCTCCTTTT GCAAGTTGGC CGGGGCTTGA GATACATACA TTCAATGTCT 
TTGGTTCACA TGGATATAAA ACCTAGTAAT ATTTTTATAT CTCGAACCTC AATCCCAAAT 
GCTGTCTCTG AGGAAGGAGA TGAAGATGAC TGGATATCCA ATAAAGTTAT GTTTAAAATA 
GGTGATCTTG GGCATGTAAC AAGAATCTCT AGTCCTCAAG TTGAAGAAGG TGATAGTCGT 
TTTCTAGCAA ATGAAGTTTT ACAAGAGAAC TATAGCCATC TACCGAAAGC AGATATTTTT 
GCTCTTGCTC TCACAGTCGT ATGTGCTGCT GGTGCTGAAC CCCTTCCCAG AAATGGAGAG 
CAGTGGCACG AGATCCGGCA GGGTCGGTTA CCTCGGATCC CACAAGTGCT TTCCCAGGAG 
GTGACAGAGT TGCTGAGAGT CATGATTCAT CCTGACCCAG AGAGAAGGCC TTCAGCAATG 
GAGCTGGTGA AGCATTCAGT GTTGTTGTCT GCATCTAGAA AGAGCGCAGA GCAGTTACGA 
ATAGAATTGA ATGCTGAAAA ATTCAAAAAT TCTCTTTTGC AGAAAGAACT CAAGAAAGCC 
CAAATGGCAG CCAAAGTTGC AGCTGAGGAA CGGGCACTCT TCACAGATCG GATGGCCACT 
AGATCCACCA CCCAGAGTAA TAGAACTTCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
GTCAGCCTTA CTATATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033542.2
CDS	292..2232
Translation	MSFLSRQQPPPTRRVGAAYSLRQKLIFSPGSDCEEEEEEEEEGSGHSTGEDSAFQEPDSPLPSARSPAEAEAERRRRSPGAEPSSPGELEDDLLLQGGGGGAQAAGGGAEGDSWEEEGFGSSSPVKSPSTAYFLSSPFSPVRCGGPGDASPQGCGAPRAMDDPCSPQPDYPSTPPHKTFRKLRLFDTPHTPKSLLSKARVIDSGSVKLRGSSLFMDTEKSGKREFDTRQTPQVNINPFTPDPVLLHSSGRCRGRKRAYFNDSSEDMEASDYEFEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHPHVVRYFSAWAEDDHMLIQNEYCNGGSLADAISENYRVMSYLTEVELKDLLLQVGRGLRYIHSMSLVHMDIKPSNIFISRTSIPNAVSEEGDEDDWISNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYSHLPKADIFALALTVVCAAGAEPLPRNGEQWHEIRQGRLPRIPQVLSQEVTELLRVMIHPDPERRPSAMELVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAAKVAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

Target ORF information:

RefSeq Version	NM_009516.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WEE 1 homolog 1 (S. pombe) (Wee1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009516.3

ORF Insert Sequence:

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCCACCCGCC GCGTAGGAGC CGCTTACAGT 
CTGCGGCAGA AGCTCATCTT CTCGCCGGGC AGCGACTGTG AGGAAGAGGA GGAGGAGGAG 
GAGGAAGGCA GCGGCCACAG TACGGGGGAG GACTCGGCCT TCCAGGAACC GGATTCGCCC 
CTGCCTTCTG CACGCAGCCC TGCCGAGGCC GAGGCCGAGC GCCGCCGTCG CTCGCCCGGC 
GCGGAGCCCA GCAGCCCGGG AGAGCTGGAG GACGACTTGC TGCTGCAGGG CGGCGGCGGA 
GGCGCGCAAG CGGCGGGTGG AGGCGCCGAG GGCGACTCTT GGGAGGAGGA GGGTTTCGGC 
TCCTCGTCGC CCGTCAAATC ACCTTCGACC GCCTACTTTC TGAGCAGCCC GTTCTCGCCA 
GTGCGCTGCG GCGGCCCAGG GGATGCCTCC CCGCAGGGTT GCGGGGCTCC GCGAGCCATG 
GACGACCCCT GCTCGCCGCA GCCCGACTAC CCGAGCACCC CTCCGCACAA GACCTTCCGC 
AAGCTGCGCC TGTTCGACAC GCCGCACACT CCCAAGAGTT TGCTTTCCAA AGCGCGAGTT 
ATTGATTCCG GCTCTGTTAA ACTCCGGGGT AGTTCTCTAT TCATGGACAC AGAAAAGTCA 
GGAAAAAGAG AATTCGATAC ACGGCAAACT CCCCAAGTGA ATATTAACCC CTTTACTCCG 
GATCCTGTAC TGCTCCATTC CTCAGGACGG TGTCGTGGGA GAAAGAGAGC GTATTTTAAT 
GATTCCTCTG AAGACATGGA AGCCAGTGAT TATGAGTTTG AAGATGAAAC AAGACCTGCC 
AAAAGAATTA CAATTACTGA AAGCAACATG AAGTCACGGT ATACAACTGA ATTTCATGAG 
CTGGAGAAAA TTGGTTCTGG AGAATTTGGT TCTGTGTTTA AATGTGTGAA GAGGTTAGAT 
GGATGCATTT ATGCCATTAA ACGATCAAAA AAACCATTGG CTGGCTCTGT TGATGAGCAG 
AATGCTTTGA GAGAAGTGTA TGCTCACGCT GTGCTTGGAC AGCACCCCCA CGTCGTTCGC 
TATTTCTCTG CCTGGGCAGA GGATGACCAC ATGCTTATAC AGAACGAATA CTGTAATGGT 
GGGAGTTTAG CTGATGCTAT AAGTGAGAAC TACAGAGTCA TGAGCTACTT GACTGAAGTA 
GAGCTGAAGG ATCTCCTTTT GCAAGTTGGC CGGGGCTTGA GATACATACA TTCAATGTCT 
TTGGTTCACA TGGATATAAA ACCTAGTAAT ATTTTTATAT CTCGAACCTC AATCCCAAAT 
GCTGTCTCTG AGGAAGGAGA TGAAGATGAC TGGATATCCA ATAAAGTTAT GTTTAAAATA 
GGTGATCTTG GGCATGTAAC AAGAATCTCT AGTCCTCAAG TTGAAGAAGG TGATAGTCGT 
TTTCTAGCAA ATGAAGTTTT ACAAGAGAAC TATAGCCATC TACCGAAAGC AGATATTTTT 
GCTCTTGCTC TCACAGTCGT ATGTGCTGCT GGTGCTGAAC CCCTTCCCAG AAATGGAGAG 
CAGTGGCACG AGATCCGGCA GGGTCGGTTA CCTCGGATCC CACAAGTGCT TTCCCAGGAG 
GTGACAGAGT TGCTGAGAGT CATGATTCAT CCTGACCCAG AGAGAAGGCC TTCAGCAATG 
GAGCTGGTGA AGCATTCAGT GTTGTTGTCT GCATCTAGAA AGAGCGCAGA GCAGTTACGA 
ATAGAATTGA ATGCTGAAAA ATTCAAAAAT TCTCTTTTGC AGAAAGAACT CAAGAAAGCC 
CAAATGGCAG CCAAAGTTGC AGCTGAGGAA CGGGCACTCT TCACAGATCG GATGGCCACT 
AGATCCACCA CCCAGAGTAA TAGAACTTCT CGACTTATTG GAAAGAAAAT GAACCGCTCT 
GTCAGCCTTA CTATATACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wee1 ( NM_001355058.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001341987.1 Mus musculus WEE 1 homolog 1 (S. pombe) (Wee1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu39454
Clone ID Related Accession (Same CDS sequence)	XM_006507585.3 , NM_001355058.1
Accession Version	NM_001355058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	wee1-like protein kinase isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC147244.2. On Sep 20, 2017 this sequence version replaced XM_006507585.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.55235.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCCACCCGCC GCGTAGGAGC CGCTTACAGT 
CTGCGGCAGA AGCTCATCTT CTCGCCGGGC AGCGACTGTG AGGAAGAGGA GGAGGAGGAG 
GAGGAAGGCA GCGGCCACAG TACGGGGGAG GACTCGGCCT TCCAGGAACC GGATTCGCCC 
CTGCCTTCTG CACGCAGCCC TGCCGAGGCC GAGGCCGAGC GCCGCCGTCG CTCGCCCGGC 
GCGGAGCCCA GCAGCCCGGG AGAGCTGGAG GACGACTTGC TGCTGCAGGG CGGCGGCGGA 
GGCGCGCAAG CGGCGGGTGG AGGCGCCGAG GGCGACTCTT GGGAGGAGGA GGGTTTCGGC 
TCCTCGTCGC CCGTCAAATC ACCTTCGACC GCCTACTTTC TGAGCAGCCC GTTCTCGCCA 
GTGCGCTGCG GCGGCCCAGG GGATGCCTCC CCGCAGGGTT GCGGGGCTCC GCGAGCCATG 
GACGACCCCT GCTCGCCGCA GCCCGACTAC CCGAGCACCC CTCCGCACAA GACCTTCCGC 
AAGCTGCGCC TGTTCGACAC GCCGCACACT CCCAAGAGTT TGCTTTCCAA AGCGCGAGTT 
ATTGATTCCG GCTCTGTTAA ACTCCGGGGT AGTTCTCTAT TCATGGACAC AGAAAAGTCA 
GGAAAAAGAG AATTCGATAC ACGGCAAACT CCCCAAGTGA ATATTAACCC CTTTACTCCG 
GATCCTGTAC TGCTCCATTC CTCAGGACGG TGTCGTGGGA GAAAGAGAGC GTATTTTAAT 
GATTCCTCTG AAGACATGGA AGCCAGTGAT TATGAGTTTG AAGATGAAAC AAGACCTGCC 
AAAAGAATTA CAATTACTGA AAGCAACATG AAGTCACGGT ATACAACTGA ATTTCATGAG 
CTGGAGAAAA TTGGTTCTGG AGAATTTGGT TCTGTGTTTA AATGTGTGAA GAGGTTAGAT 
GGATGCATTT ATGCCATTAA ACGATCAAAA AAACCATTGG CTGGCTCTGT TGATGAGCAG 
AATGCTTTGA GAGAAGTGTA TGCTCACGCT GTGCTTGGAC AGCACCCCCA CGTCGTTCGC 
TATTTCTCTG CCTGGGCAGA GGATGACCAC ATGCTTATAC AGAACGAATA CTGTAATGGT 
AATATTTTTA TATCTCGAAC CTCAATCCCA AATGCTGTCT CTGAGGAAGG AGATGAAGAT 
GACTGGATAT CCAATAAAGT TATGTTTAAA ATAGGTGATC TTGGGCATGT AACAAGAATC 
TCTAGTCCTC AAGTTGAAGA AGGTGATAGT CGTTTTCTAG CAAATGAAGT TTTACAAGAG 
AACTATAGCC ATCTACCGAA AGCAGATATT TTTGCTCTTG CTCTCACAGT CGTATGTGCT 
GCTGGTGCTG AACCCCTTCC CAGAAATGGA GAGCAGTGGC ACGAGATCCG GCAGGGTCGG 
TTACCTCGGA TCCCACAAGT GCTTTCCCAG GAGGTGACAG AGTTGCTGAG AGTCATGATT 
CATCCTGACC CAGAGAGAAG GCCTTCAGCA ATGGAGCTGG TGAAGCATTC AGTGTTGTTG 
TCTGCATCTA GAAAGAGCGC AGAGCAGTTA CGAATAGAAT TGAATGCTGA AAAATTCAAA 
AATTCTCTTT TGCAGAAAGA ACTCAAGAAA GCCCAAATGG CAGCCAAAGT TGCAGCTGAG 
GAACGGGCAC TCTTCACAGA TCGGATGGCC ACTAGATCCA CCACCCAGAG TAATAGAACT 
TCTCGACTTA TTGGAAAGAA AATGAACCGC TCTGTCAGCC TTACTATATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341987.1
CDS	292..2085
Translation	MSFLSRQQPPPTRRVGAAYSLRQKLIFSPGSDCEEEEEEEEEGSGHSTGEDSAFQEPDSPLPSARSPAEAEAERRRRSPGAEPSSPGELEDDLLLQGGGGGAQAAGGGAEGDSWEEEGFGSSSPVKSPSTAYFLSSPFSPVRCGGPGDASPQGCGAPRAMDDPCSPQPDYPSTPPHKTFRKLRLFDTPHTPKSLLSKARVIDSGSVKLRGSSLFMDTEKSGKREFDTRQTPQVNINPFTPDPVLLHSSGRCRGRKRAYFNDSSEDMEASDYEFEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHPHVVRYFSAWAEDDHMLIQNEYCNGNIFISRTSIPNAVSEEGDEDDWISNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYSHLPKADIFALALTVVCAAGAEPLPRNGEQWHEIRQGRLPRIPQVLSQEVTELLRVMIHPDPERRPSAMELVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAAKVAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

Target ORF information:

RefSeq Version	NM_001355058.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WEE 1 homolog 1 (S. pombe) (Wee1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355058.1

ORF Insert Sequence:

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCCACCCGCC GCGTAGGAGC CGCTTACAGT 
CTGCGGCAGA AGCTCATCTT CTCGCCGGGC AGCGACTGTG AGGAAGAGGA GGAGGAGGAG 
GAGGAAGGCA GCGGCCACAG TACGGGGGAG GACTCGGCCT TCCAGGAACC GGATTCGCCC 
CTGCCTTCTG CACGCAGCCC TGCCGAGGCC GAGGCCGAGC GCCGCCGTCG CTCGCCCGGC 
GCGGAGCCCA GCAGCCCGGG AGAGCTGGAG GACGACTTGC TGCTGCAGGG CGGCGGCGGA 
GGCGCGCAAG CGGCGGGTGG AGGCGCCGAG GGCGACTCTT GGGAGGAGGA GGGTTTCGGC 
TCCTCGTCGC CCGTCAAATC ACCTTCGACC GCCTACTTTC TGAGCAGCCC GTTCTCGCCA 
GTGCGCTGCG GCGGCCCAGG GGATGCCTCC CCGCAGGGTT GCGGGGCTCC GCGAGCCATG 
GACGACCCCT GCTCGCCGCA GCCCGACTAC CCGAGCACCC CTCCGCACAA GACCTTCCGC 
AAGCTGCGCC TGTTCGACAC GCCGCACACT CCCAAGAGTT TGCTTTCCAA AGCGCGAGTT 
ATTGATTCCG GCTCTGTTAA ACTCCGGGGT AGTTCTCTAT TCATGGACAC AGAAAAGTCA 
GGAAAAAGAG AATTCGATAC ACGGCAAACT CCCCAAGTGA ATATTAACCC CTTTACTCCG 
GATCCTGTAC TGCTCCATTC CTCAGGACGG TGTCGTGGGA GAAAGAGAGC GTATTTTAAT 
GATTCCTCTG AAGACATGGA AGCCAGTGAT TATGAGTTTG AAGATGAAAC AAGACCTGCC 
AAAAGAATTA CAATTACTGA AAGCAACATG AAGTCACGGT ATACAACTGA ATTTCATGAG 
CTGGAGAAAA TTGGTTCTGG AGAATTTGGT TCTGTGTTTA AATGTGTGAA GAGGTTAGAT 
GGATGCATTT ATGCCATTAA ACGATCAAAA AAACCATTGG CTGGCTCTGT TGATGAGCAG 
AATGCTTTGA GAGAAGTGTA TGCTCACGCT GTGCTTGGAC AGCACCCCCA CGTCGTTCGC 
TATTTCTCTG CCTGGGCAGA GGATGACCAC ATGCTTATAC AGAACGAATA CTGTAATGGT 
AATATTTTTA TATCTCGAAC CTCAATCCCA AATGCTGTCT CTGAGGAAGG AGATGAAGAT 
GACTGGATAT CCAATAAAGT TATGTTTAAA ATAGGTGATC TTGGGCATGT AACAAGAATC 
TCTAGTCCTC AAGTTGAAGA AGGTGATAGT CGTTTTCTAG CAAATGAAGT TTTACAAGAG 
AACTATAGCC ATCTACCGAA AGCAGATATT TTTGCTCTTG CTCTCACAGT CGTATGTGCT 
GCTGGTGCTG AACCCCTTCC CAGAAATGGA GAGCAGTGGC ACGAGATCCG GCAGGGTCGG 
TTACCTCGGA TCCCACAAGT GCTTTCCCAG GAGGTGACAG AGTTGCTGAG AGTCATGATT 
CATCCTGACC CAGAGAGAAG GCCTTCAGCA ATGGAGCTGG TGAAGCATTC AGTGTTGTTG 
TCTGCATCTA GAAAGAGCGC AGAGCAGTTA CGAATAGAAT TGAATGCTGA AAAATTCAAA 
AATTCTCTTT TGCAGAAAGA ACTCAAGAAA GCCCAAATGG CAGCCAAAGT TGCAGCTGAG 
GAACGGGCAC TCTTCACAGA TCGGATGGCC ACTAGATCCA CCACCCAGAG TAATAGAACT 
TCTCGACTTA TTGGAAAGAA AATGAACCGC TCTGTCAGCC TTACTATATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wee1 ( XM_006507585.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006507648.1 Mus musculus WEE 1 homolog 1 (S. pombe) (Wee1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39454
Clone ID Related Accession (Same CDS sequence)	XM_006507585.3 , NM_001355058.1
Accession Version	XM_006507585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	wee1-like protein kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCCACCCGCC GCGTAGGAGC CGCTTACAGT 
CTGCGGCAGA AGCTCATCTT CTCGCCGGGC AGCGACTGTG AGGAAGAGGA GGAGGAGGAG 
GAGGAAGGCA GCGGCCACAG TACGGGGGAG GACTCGGCCT TCCAGGAACC GGATTCGCCC 
CTGCCTTCTG CACGCAGCCC TGCCGAGGCC GAGGCCGAGC GCCGCCGTCG CTCGCCCGGC 
GCGGAGCCCA GCAGCCCGGG AGAGCTGGAG GACGACTTGC TGCTGCAGGG CGGCGGCGGA 
GGCGCGCAAG CGGCGGGTGG AGGCGCCGAG GGCGACTCTT GGGAGGAGGA GGGTTTCGGC 
TCCTCGTCGC CCGTCAAATC ACCTTCGACC GCCTACTTTC TGAGCAGCCC GTTCTCGCCA 
GTGCGCTGCG GCGGCCCAGG GGATGCCTCC CCGCAGGGTT GCGGGGCTCC GCGAGCCATG 
GACGACCCCT GCTCGCCGCA GCCCGACTAC CCGAGCACCC CTCCGCACAA GACCTTCCGC 
AAGCTGCGCC TGTTCGACAC GCCGCACACT CCCAAGAGTT TGCTTTCCAA AGCGCGAGTT 
ATTGATTCCG GCTCTGTTAA ACTCCGGGGT AGTTCTCTAT TCATGGACAC AGAAAAGTCA 
GGAAAAAGAG AATTCGATAC ACGGCAAACT CCCCAAGTGA ATATTAACCC CTTTACTCCG 
GATCCTGTAC TGCTCCATTC CTCAGGACGG TGTCGTGGGA GAAAGAGAGC GTATTTTAAT 
GATTCCTCTG AAGACATGGA AGCCAGTGAT TATGAGTTTG AAGATGAAAC AAGACCTGCC 
AAAAGAATTA CAATTACTGA AAGCAACATG AAGTCACGGT ATACAACTGA ATTTCATGAG 
CTGGAGAAAA TTGGTTCTGG AGAATTTGGT TCTGTGTTTA AATGTGTGAA GAGGTTAGAT 
GGATGCATTT ATGCCATTAA ACGATCAAAA AAACCATTGG CTGGCTCTGT TGATGAGCAG 
AATGCTTTGA GAGAAGTGTA TGCTCACGCT GTGCTTGGAC AGCACCCCCA CGTCGTTCGC 
TATTTCTCTG CCTGGGCAGA GGATGACCAC ATGCTTATAC AGAACGAATA CTGTAATGGT 
AATATTTTTA TATCTCGAAC CTCAATCCCA AATGCTGTCT CTGAGGAAGG AGATGAAGAT 
GACTGGATAT CCAATAAAGT TATGTTTAAA ATAGGTGATC TTGGGCATGT AACAAGAATC 
TCTAGTCCTC AAGTTGAAGA AGGTGATAGT CGTTTTCTAG CAAATGAAGT TTTACAAGAG 
AACTATAGCC ATCTACCGAA AGCAGATATT TTTGCTCTTG CTCTCACAGT CGTATGTGCT 
GCTGGTGCTG AACCCCTTCC CAGAAATGGA GAGCAGTGGC ACGAGATCCG GCAGGGTCGG 
TTACCTCGGA TCCCACAAGT GCTTTCCCAG GAGGTGACAG AGTTGCTGAG AGTCATGATT 
CATCCTGACC CAGAGAGAAG GCCTTCAGCA ATGGAGCTGG TGAAGCATTC AGTGTTGTTG 
TCTGCATCTA GAAAGAGCGC AGAGCAGTTA CGAATAGAAT TGAATGCTGA AAAATTCAAA 
AATTCTCTTT TGCAGAAAGA ACTCAAGAAA GCCCAAATGG CAGCCAAAGT TGCAGCTGAG 
GAACGGGCAC TCTTCACAGA TCGGATGGCC ACTAGATCCA CCACCCAGAG TAATAGAACT 
TCTCGACTTA TTGGAAAGAA AATGAACCGC TCTGTCAGCC TTACTATATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006507648.1
CDS	369..2162
Translation	MSFLSRQQPPPTRRVGAAYSLRQKLIFSPGSDCEEEEEEEEEGSGHSTGEDSAFQEPDSPLPSARSPAEAEAERRRRSPGAEPSSPGELEDDLLLQGGGGGAQAAGGGAEGDSWEEEGFGSSSPVKSPSTAYFLSSPFSPVRCGGPGDASPQGCGAPRAMDDPCSPQPDYPSTPPHKTFRKLRLFDTPHTPKSLLSKARVIDSGSVKLRGSSLFMDTEKSGKREFDTRQTPQVNINPFTPDPVLLHSSGRCRGRKRAYFNDSSEDMEASDYEFEDETRPAKRITITESNMKSRYTTEFHELEKIGSGEFGSVFKCVKRLDGCIYAIKRSKKPLAGSVDEQNALREVYAHAVLGQHPHVVRYFSAWAEDDHMLIQNEYCNGNIFISRTSIPNAVSEEGDEDDWISNKVMFKIGDLGHVTRISSPQVEEGDSRFLANEVLQENYSHLPKADIFALALTVVCAAGAEPLPRNGEQWHEIRQGRLPRIPQVLSQEVTELLRVMIHPDPERRPSAMELVKHSVLLSASRKSAEQLRIELNAEKFKNSLLQKELKKAQMAAKVAAEERALFTDRMATRSTTQSNRTSRLIGKKMNRSVSLTIY

Target ORF information:

RefSeq Version	XM_006507585.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus WEE 1 homolog 1 (S. pombe) (Wee1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006507585.3

ORF Insert Sequence:

ATGAGCTTCC TGAGCCGACA GCAGCCGCCG CCCACCCGCC GCGTAGGAGC CGCTTACAGT 
CTGCGGCAGA AGCTCATCTT CTCGCCGGGC AGCGACTGTG AGGAAGAGGA GGAGGAGGAG 
GAGGAAGGCA GCGGCCACAG TACGGGGGAG GACTCGGCCT TCCAGGAACC GGATTCGCCC 
CTGCCTTCTG CACGCAGCCC TGCCGAGGCC GAGGCCGAGC GCCGCCGTCG CTCGCCCGGC 
GCGGAGCCCA GCAGCCCGGG AGAGCTGGAG GACGACTTGC TGCTGCAGGG CGGCGGCGGA 
GGCGCGCAAG CGGCGGGTGG AGGCGCCGAG GGCGACTCTT GGGAGGAGGA GGGTTTCGGC 
TCCTCGTCGC CCGTCAAATC ACCTTCGACC GCCTACTTTC TGAGCAGCCC GTTCTCGCCA 
GTGCGCTGCG GCGGCCCAGG GGATGCCTCC CCGCAGGGTT GCGGGGCTCC GCGAGCCATG 
GACGACCCCT GCTCGCCGCA GCCCGACTAC CCGAGCACCC CTCCGCACAA GACCTTCCGC 
AAGCTGCGCC TGTTCGACAC GCCGCACACT CCCAAGAGTT TGCTTTCCAA AGCGCGAGTT 
ATTGATTCCG GCTCTGTTAA ACTCCGGGGT AGTTCTCTAT TCATGGACAC AGAAAAGTCA 
GGAAAAAGAG AATTCGATAC ACGGCAAACT CCCCAAGTGA ATATTAACCC CTTTACTCCG 
GATCCTGTAC TGCTCCATTC CTCAGGACGG TGTCGTGGGA GAAAGAGAGC GTATTTTAAT 
GATTCCTCTG AAGACATGGA AGCCAGTGAT TATGAGTTTG AAGATGAAAC AAGACCTGCC 
AAAAGAATTA CAATTACTGA AAGCAACATG AAGTCACGGT ATACAACTGA ATTTCATGAG 
CTGGAGAAAA TTGGTTCTGG AGAATTTGGT TCTGTGTTTA AATGTGTGAA GAGGTTAGAT 
GGATGCATTT ATGCCATTAA ACGATCAAAA AAACCATTGG CTGGCTCTGT TGATGAGCAG 
AATGCTTTGA GAGAAGTGTA TGCTCACGCT GTGCTTGGAC AGCACCCCCA CGTCGTTCGC 
TATTTCTCTG CCTGGGCAGA GGATGACCAC ATGCTTATAC AGAACGAATA CTGTAATGGT 
AATATTTTTA TATCTCGAAC CTCAATCCCA AATGCTGTCT CTGAGGAAGG AGATGAAGAT 
GACTGGATAT CCAATAAAGT TATGTTTAAA ATAGGTGATC TTGGGCATGT AACAAGAATC 
TCTAGTCCTC AAGTTGAAGA AGGTGATAGT CGTTTTCTAG CAAATGAAGT TTTACAAGAG 
AACTATAGCC ATCTACCGAA AGCAGATATT TTTGCTCTTG CTCTCACAGT CGTATGTGCT 
GCTGGTGCTG AACCCCTTCC CAGAAATGGA GAGCAGTGGC ACGAGATCCG GCAGGGTCGG 
TTACCTCGGA TCCCACAAGT GCTTTCCCAG GAGGTGACAG AGTTGCTGAG AGTCATGATT 
CATCCTGACC CAGAGAGAAG GCCTTCAGCA ATGGAGCTGG TGAAGCATTC AGTGTTGTTG 
TCTGCATCTA GAAAGAGCGC AGAGCAGTTA CGAATAGAAT TGAATGCTGA AAAATTCAAA 
AATTCTCTTT TGCAGAAAGA ACTCAAGAAA GCCCAAATGG CAGCCAAAGT TGCAGCTGAG 
GAACGGGCAC TCTTCACAGA TCGGATGGCC ACTAGATCCA CCACCCAGAG TAATAGAACT 
TCTCGACTTA TTGGAAAGAA AATGAACCGC TCTGTCAGCC TTACTATATA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lacking of palladin leads to multiple cellular events changes which contribute to NTD.
Neural development12(1)4(2017 Mar)
Tan J,Chen XJ,Shen CL,Zhang HX,Tang LY,Lu SY,Wu WT,Kuang Y,Fei J,Wang ZG

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

MicroRNA-219-5p Inhibits Morphine-Induced Apoptosis by Targeting Key Cell Cycle Regulator WEE1.
Medical science monitor : international medical journal of experimental and clinical research221872-9(2016 Jun)
Lou W,Zhang X,Hu XY,Hu AR

WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis.
Oncogene34(23)3023-35(2015 Jun)
Vassilopoulos A,Tominaga Y,Kim HS,Lahusen T,Li B,Yu H,Gius D,Deng CX

Involvement of Wee1 in the circadian rhythm-dependent intestinal damage induced by docetaxel.
The Journal of pharmacology and experimental therapeutics347(1)242-8(2013 Oct)
Obi-Ioka Y,Ushijima K,Kusama M,Ishikawa-Kobayashi E,Fujimura A

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Wee1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

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